LOC7468676 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7468676
Entrez Gene ID	7468676
Full Name	GYMNOS family protein
Synonyms	POPTRDRAFT_415868
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002309629.3	XP_002309665.2	CHD3-type chromatin-remodeling factor PICKLE isoform X4
XM_024603305.1	XP_024459073.1	CHD3-type chromatin-remodeling factor PICKLE isoform X1
XM_024603307.1	XP_024459075.1	CHD3-type chromatin-remodeling factor PICKLE isoform X3
XM_002309629.2	XP_002309665.2	GYMNOS family protein
XM_024603306.1	XP_024459074.1	CHD3-type chromatin-remodeling factor PICKLE isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7468676 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7468676 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa14654	XM_002309629.3 Latest version!	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa431574	XM_024603305.1 Latest version!	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa431575	XM_024603306.1 Latest version!	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa431576	XM_024603307.1 Latest version!	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OPa14654	XM_002309629.2	Populus trichocarpa GYMNOS family protein (POPTR_0006s27810g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7468676 ( XM_002309629.3 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002309665.2 Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa14654
Clone ID Related Accession (Same CDS sequence)	XM_002309629.3 , XM_002309629.2
Accession Version	XM_002309629.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	CHD3-type chromatin-remodeling factor PICKLE isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002309629.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCT TAGTGGAGAG GCTACGTGTT AGATCAGAAA GGAGGCCAGT TTATAACTTG 
GATGAATCAG ACGATGATGA TTATGTTTCC GGGAAAGCTA AAAATCCCCA GGAGAAAATT 
GAAAGATTTG TCAGGGATGA CGCGAAGGAG GATTCCTGTC AAGCTTGTGG AGAAAGTGAG 
AATCTTCTGA ATTGTGAAAC CTGCACATAT GCTTACCATT CCAAGTGCCT GCTTCCACCA 
CTAAAAGCTC CTTTTCCTAG CAATTGGAGG TGCCCTGAAT GTGTTAGCCC TCTGAATGAT 
ATCGATAAGT TATTGGACTG TGAAATGCGC CCTACTGTTG CTGATGATAG CGATGCCTCT 
AAACTGGGCT CAAAGCAAAT TTTTGTAAAA CAGTATCTTG TGAAATGGAA AGGCTTATCT 
TATCTACATT GCACTTGGGT ACCTGAGAAA GAGTTTTTAA AAGCTTTCAA GTCAAATCCC 
CGTCTGAAGA CTAAAGTAAA CAACTTCAAT CGTCAGATGG CATCAAACAA CAACTCCGAG 
GATGAATTTG TTGCTATCCG ACCTGAATGG ACAACTGTTG ATCGGATTCT TGCTTGCAGG 
GGAGATGAGG ATGAGAAGGA ATATCTTGTG AAGTACAAGG AACTCCCTTA CGATGAATGT 
TATTGGGAAT TTGAATCAGA TGTATCTGCA TTTCAGCCTG AAATAGAAAA ATTTAATAAA 
ATTCAATCTA GGTCTCACAA ACCGAGCAAG CAAAAGAGCA GCCTTCAAGA TGCTACTGAT 
TCAAAGAAGA AATCAAAAGA ATTTCAACAG TGTGATCACA GTCCTGAATT TCTTTCTGGA 
GGCTCATTGC ATCCTTATCA GTTAGAAGGG CTGAACTTCT TACGTTTTTC TTGGTCCAAA 
CAAACACATG TAATACTTGC TGATGAGATG GGCCTTGGGA AAACTATACA GAGTATTGCA 
TTTTTAGCAT CTCTTCGCGA AGAGGGCATC TCTCCGTATC TGGTAGTAGC TCCACTTTCA 
ACATTGCGAA ACTGGGAACG TGAATTTGCA ACATGGGCAC CTCAAATGAA TGTTGTTATG 
TATGTTGGCT CCGCACAAGC TCGTGCTGTC ATAAGGGAAT ATGAGTTTTA CTATCCCAAA 
AATCACAAGA AGATCAAGAA AAAGAAATCT GGTCAAGTTG TTACTGAAAG CAAGCAAGAT 
AGAATAAAAT TTGATGTGCT TCTAACGTCT TATGAGATGA TTAACTTGGA CTCAACATCG 
CTGAAACCAA TAAAGTGGGA ATGCATGATT GTTGATGAAG GTCATCGTCT AAAAAACAAG 
GACTCTAAAT TGTTTCTCTC GCTGAAGCAG TACTGTAGTA ACCATCGTGT GCTTTTGACT 
GGAACCCCTC TTCAGAACAA TCTGGATGAA CTTTTCATGC TCATGCATTT CCTTGATGCT 
GGGAAGTTTG CAAGTTTAGA GGAATTCCAA GAGGAGTTTA AGGATATAAA TCAAGAGGAG 
CAGATTTTGA GACTTCATAA AATGTTGGCT CCCCATCTCC TCAGAAGGGT GAAAAAGGAT 
GTCATGAAAG AGCTACCTCC TAAGAAGGAA CTCATTCTAC GTATTGAACT AAGTAGCAAG 
CAGAAAGAAT ATTACAAGGC CATTCTGACT CGCAACTATC AAATACTAAC TCGACGTGGT 
GGTGCACAGA TTTCTCTTAT CAATGTGGTT ATGGAATTGC GCAAACTCTG TTGTCATCCT 
TATATGTTAG AAGGAGTGGA GCCGGATATT GAAGATACCA ATGAATCTTT CAGGCAGCTC 
CTTGAAACTT CTGGGAAATT GCAACTGTTG GACAAACTGA TGGTGAGACT TAAAGAACAA 
GGGCATAGAG TTCTCATTTA CTCACAATTT CAGCACATGC TGGACTTGCT AGAAGATTAC 
TGCACTCATA AGAAATGGAT GTATGAACGG ATAGATGGAA AGGTTGGTGG AGCTGAGAGA 
CAAGTGCGCA TAGATCGATT TAATGCCAAA AATTCTTCAA GATTTTGCTT TCTTCTCTCA 
ACTAGAGCCG GAGGACTGGG AATAAACCTT GCAACTGCTG ATACTGTTAT TATATATGAT 
AGTGATTGGA ATCCCCATGC TGATCTTCAA GCAATGGCTA GAGCACATCG ACTTGGGCAA 
ACCAACAAGG TATTGATTTA TAGGCTCATA ACCCGAGGAA CTATTGAGGA ACGGATGATG 
CAACTGACTA AGAAGAAAAT GGTTTTAGAG CACCTTGTTG TTGGGAGGCT GAAGGCACAG 
AATATTAATC AGGAAGAGTT AGATGACATC ATAAGATATG GTTCAAAGGA GCTTTTTGCT 
GATGAGAATG ATGAAGCAGG GAAATCACGT CAAATTCATT ATGATGATGC TGCTATTGAT 
CGATTACTTG ACCGTGAACA AGTTGGAGAT GAAGAAACAT CATTGGATGA TGAAGAGGAA 
GATGGATTTT TGAAGGCGTT TAAGGTTGCA AATTTTGAGT ATATTGACGA AGCAGAGGCT 
GCCGCAGAGG AGGAAGCTCA AAAGGCAGCC ATGGAAACGA GATCCACCAT AAACAATTCT 
GAAAGAACTG AAAAAACAAA CTTTTGGGAA GAGTTGCTAA AAGATAGTTA TGAAGTACAC 
AAAGTTGAGG AGTTTAATGC CTTGGGCAAA GGAAAGAGAA GCCGTAAGCA GATGGTGTCT 
GTTGAAGATG ATGACCTTGC TGGTCTAGAA GATGTAAGCT CTGATGGCGA GGATGACAAT 
TATGAAGCTG AGTTGACTGA TGGTGAAACA ACATCATCAG GAGTAGTTCA GACAGTAAGA 
AGGCCTTATA AGAAGAAAGC TCGAGTGGAT AATACAGAAC CAATTCCTCT GATGGAAGGT 
GAAGGAAGAT CATTCAGAGT ATTGGGTTTT AAACAAAATC AGAGGGCTGC CTTTGTACAA 
ATCTTGATGA GGTTTGGGGT TGGGGACTAT GACTGGAAGG AGTTTGCCTC TCGATTGAAA 
CAGAAGACAT ATGAAGAAGT AGAAAATTAT GGGAGATTAT TCTTGACTCA CATAGCGGAA 
GATCTAACTG ACTCACCAAA TTTTTCAGAC GGTGTTCCTA AAGAGGGTCT TAGAATACAA 
GACGTACTTG TTAGGATTGC TGTCCTGCTT TTGATAAGGG ATAAGGCGAG GTTTGCATCA 
GAGAACCCAG GTAGCGCCCT TTTCACAGAT GACATTATAC TGCGCTACCC AGGCCTGAAG 
AGTGGAAAAT TTTGGAAACA AGAGCACGAT TCATTGTTAC TTCATGCAGT ATTGAAGCAT 
GGGTATGGAA GATGGCAAGC TATTGTTGAT GACAAGGACT TGAAGGTTCA AGAGATAATC 
TGTAAAGAGT TGAATCTTCC TTGTATAAGA CTACCTGTCC TTGGGCAAGG AGTTGCTCAA 
GCACAAAATG GTTCTACTTC TAACATTGCT AATGCAGAAG CTCCTAGCAC CCAGGCACAG 
GCAAATGTTG CTGGAAATGA TGTAGCAGCT GATGTTGCTC AGGGGACAAT TGATGCTGCA 
AACCCAGCAC TGTCATATCG AGATTCATCT ATATTATTTC ATTTTAGAGA TATGCAGAGA 
CGACAGGTAG AGTTCATTAA GAAAAGAGTG CTGCTTTTAG AGAGAGGACT CAATGCGGAG 
TACCAGAAAA TTTACTTTGG TGGTGATATA AAACCAAATG AGATTACAAG TGAAGAGGCT 
GATGGTGAAA CAAAGGCTGC TGACAGCTCA AGTTTAGGCT CCATAGAGAT CAATGCTCAA 
ATGATTGACC AGTTGCCTCA GATGGAACCA ATTGGATCAG AAGAAATTTC AGCAGCTGCT 
TGTGATGACA ATCCTGATAG ATTGGCATTG GCTGAGCATT ATAACAAGAT GTGCACGGTG 
TTGGAGCAAA ATGTTCATGA AACAATTCAG ATATCCTTAA CCAACCACCC TGCAAGTCTC 
AAGTTGAGAC AGGGCCTTCA GCCATTAGAG ATGATTTTTG AACAGATGAA TCAAATTCTC 
TCTCCTTTAC AACAAAAATC TACTTCGGAA CAAGGTACTC TGGGACCGAA CAAACATGTG 
CAAGCTGAAT CACAGAGCAA TCAGGCTAAA CTTCATTCAC CATCTGACCA ACAGAAGGAG 
AATAACGACA ATGCTGCTGC TGTGGAAGAT GTTGAAATGA AAGAGGCAAC GACAGAGCCA 
AAATTGGAAG AAACCATTGC ATCTTCGGAT GAAGAAGCTC CACATTCTGC AGACCCAGTT 
ACGCCTCCAA AAGAACCTAT GTGTTCACCA GGGACATCTG AAAAGGATGT GCAAATGGTC 
GATACAAGTA ATGGTACTGA CACCAACACT GATGCTGTAT CAAATGAAAA TGAAACCACA 
GAGAAATCAA ATTCGGGGGT GATCGTTTTA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002309665.2
CDS	162..4577
Translation	MSSLVERLRVRSERRPVYNLDESDDDDYVSGKAKNPQEKIERFVRDDAKEDSCQACGESENLLNCETCTYAYHSKCLLPPLKAPFPSNWRCPECVSPLNDIDKLLDCEMRPTVADDSDASKLGSKQIFVKQYLVKWKGLSYLHCTWVPEKEFLKAFKSNPRLKTKVNNFNRQMASNNNSEDEFVAIRPEWTTVDRILACRGDEDEKEYLVKYKELPYDECYWEFESDVSAFQPEIEKFNKIQSRSHKPSKQKSSLQDATDSKKKSKEFQQCDHSPEFLSGGSLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLREEGISPYLVVAPLSTLRNWEREFATWAPQMNVVMYVGSAQARAVIREYEFYYPKNHKKIKKKKSGQVVTESKQDRIKFDVLLTSYEMINLDSTSLKPIKWECMIVDEGHRLKNKDSKLFLSLKQYCSNHRVLLTGTPLQNNLDELFMLMHFLDAGKFASLEEFQEEFKDINQEEQILRLHKMLAPHLLRRVKKDVMKELPPKKELILRIELSSKQKEYYKAILTRNYQILTRRGGAQISLINVVMELRKLCCHPYMLEGVEPDIEDTNESFRQLLETSGKLQLLDKLMVRLKEQGHRVLIYSQFQHMLDLLEDYCTHKKWMYERIDGKVGGAERQVRIDRFNAKNSSRFCFLLSTRAGGLGINLATADTVIIYDSDWNPHADLQAMARAHRLGQTNKVLIYRLITRGTIEERMMQLTKKKMVLEHLVVGRLKAQNINQEELDDIIRYGSKELFADENDEAGKSRQIHYDDAAIDRLLDREQVGDEETSLDDEEEDGFLKAFKVANFEYIDEAEAAAEEEAQKAAMETRSTINNSERTEKTNFWEELLKDSYEVHKVEEFNALGKGKRSRKQMVSVEDDDLAGLEDVSSDGEDDNYEAELTDGETTSSGVVQTVRRPYKKKARVDNTEPIPLMEGEGRSFRVLGFKQNQRAAFVQILMRFGVGDYDWKEFASRLKQKTYEEVENYGRLFLTHIAEDLTDSPNFSDGVPKEGLRIQDVLVRIAVLLLIRDKARFASENPGSALFTDDIILRYPGLKSGKFWKQEHDSLLLHAVLKHGYGRWQAIVDDKDLKVQEIICKELNLPCIRLPVLGQGVAQAQNGSTSNIANAEAPSTQAQANVAGNDVAADVAQGTIDAANPALSYRDSSILFHFRDMQRRQVEFIKKRVLLLERGLNAEYQKIYFGGDIKPNEITSEEADGETKAADSSSLGSIEINAQMIDQLPQMEPIGSEEISAAACDDNPDRLALAEHYNKMCTVLEQNVHETIQISLTNHPASLKLRQGLQPLEMIFEQMNQILSPLQQKSTSEQGTLGPNKHVQAESQSNQAKLHSPSDQQKENNDNAAAVEDVEMKEATTEPKLEETIASSDEEAPHSADPVTPPKEPMCSPGTSEKDVQMVDTSNGTDTNTDAVSNENETTEKSNSGVIVLD

Target ORF information:

RefSeq Version	XM_002309629.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002309629.3

ORF Insert Sequence:

ATGAGTAGCT TAGTGGAGAG GCTACGTGTT AGATCAGAAA GGAGGCCAGT TTATAACTTG 
GATGAATCAG ACGATGATGA TTATGTTTCC GGGAAAGCTA AAAATCCCCA GGAGAAAATT 
GAAAGATTTG TCAGGGATGA CGCGAAGGAG GATTCCTGTC AAGCTTGTGG AGAAAGTGAG 
AATCTTCTGA ATTGTGAAAC CTGCACATAT GCTTACCATT CCAAGTGCCT GCTTCCACCA 
CTAAAAGCTC CTTTTCCTAG CAATTGGAGG TGCCCTGAAT GTGTTAGCCC TCTGAATGAT 
ATCGATAAGT TATTGGACTG TGAAATGCGC CCTACTGTTG CTGATGATAG CGATGCCTCT 
AAACTGGGCT CAAAGCAAAT TTTTGTAAAA CAGTATCTTG TGAAATGGAA AGGCTTATCT 
TATCTACATT GCACTTGGGT ACCTGAGAAA GAGTTTTTAA AAGCTTTCAA GTCAAATCCC 
CGTCTGAAGA CTAAAGTAAA CAACTTCAAT CGTCAGATGG CATCAAACAA CAACTCCGAG 
GATGAATTTG TTGCTATCCG ACCTGAATGG ACAACTGTTG ATCGGATTCT TGCTTGCAGG 
GGAGATGAGG ATGAGAAGGA ATATCTTGTG AAGTACAAGG AACTCCCTTA CGATGAATGT 
TATTGGGAAT TTGAATCAGA TGTATCTGCA TTTCAGCCTG AAATAGAAAA ATTTAATAAA 
ATTCAATCTA GGTCTCACAA ACCGAGCAAG CAAAAGAGCA GCCTTCAAGA TGCTACTGAT 
TCAAAGAAGA AATCAAAAGA ATTTCAACAG TGTGATCACA GTCCTGAATT TCTTTCTGGA 
GGCTCATTGC ATCCTTATCA GTTAGAAGGG CTGAACTTCT TACGTTTTTC TTGGTCCAAA 
CAAACACATG TAATACTTGC TGATGAGATG GGCCTTGGGA AAACTATACA GAGTATTGCA 
TTTTTAGCAT CTCTTCGCGA AGAGGGCATC TCTCCGTATC TGGTAGTAGC TCCACTTTCA 
ACATTGCGAA ACTGGGAACG TGAATTTGCA ACATGGGCAC CTCAAATGAA TGTTGTTATG 
TATGTTGGCT CCGCACAAGC TCGTGCTGTC ATAAGGGAAT ATGAGTTTTA CTATCCCAAA 
AATCACAAGA AGATCAAGAA AAAGAAATCT GGTCAAGTTG TTACTGAAAG CAAGCAAGAT 
AGAATAAAAT TTGATGTGCT TCTAACGTCT TATGAGATGA TTAACTTGGA CTCAACATCG 
CTGAAACCAA TAAAGTGGGA ATGCATGATT GTTGATGAAG GTCATCGTCT AAAAAACAAG 
GACTCTAAAT TGTTTCTCTC GCTGAAGCAG TACTGTAGTA ACCATCGTGT GCTTTTGACT 
GGAACCCCTC TTCAGAACAA TCTGGATGAA CTTTTCATGC TCATGCATTT CCTTGATGCT 
GGGAAGTTTG CAAGTTTAGA GGAATTCCAA GAGGAGTTTA AGGATATAAA TCAAGAGGAG 
CAGATTTTGA GACTTCATAA AATGTTGGCT CCCCATCTCC TCAGAAGGGT GAAAAAGGAT 
GTCATGAAAG AGCTACCTCC TAAGAAGGAA CTCATTCTAC GTATTGAACT AAGTAGCAAG 
CAGAAAGAAT ATTACAAGGC CATTCTGACT CGCAACTATC AAATACTAAC TCGACGTGGT 
GGTGCACAGA TTTCTCTTAT CAATGTGGTT ATGGAATTGC GCAAACTCTG TTGTCATCCT 
TATATGTTAG AAGGAGTGGA GCCGGATATT GAAGATACCA ATGAATCTTT CAGGCAGCTC 
CTTGAAACTT CTGGGAAATT GCAACTGTTG GACAAACTGA TGGTGAGACT TAAAGAACAA 
GGGCATAGAG TTCTCATTTA CTCACAATTT CAGCACATGC TGGACTTGCT AGAAGATTAC 
TGCACTCATA AGAAATGGAT GTATGAACGG ATAGATGGAA AGGTTGGTGG AGCTGAGAGA 
CAAGTGCGCA TAGATCGATT TAATGCCAAA AATTCTTCAA GATTTTGCTT TCTTCTCTCA 
ACTAGAGCCG GAGGACTGGG AATAAACCTT GCAACTGCTG ATACTGTTAT TATATATGAT 
AGTGATTGGA ATCCCCATGC TGATCTTCAA GCAATGGCTA GAGCACATCG ACTTGGGCAA 
ACCAACAAGG TATTGATTTA TAGGCTCATA ACCCGAGGAA CTATTGAGGA ACGGATGATG 
CAACTGACTA AGAAGAAAAT GGTTTTAGAG CACCTTGTTG TTGGGAGGCT GAAGGCACAG 
AATATTAATC AGGAAGAGTT AGATGACATC ATAAGATATG GTTCAAAGGA GCTTTTTGCT 
GATGAGAATG ATGAAGCAGG GAAATCACGT CAAATTCATT ATGATGATGC TGCTATTGAT 
CGATTACTTG ACCGTGAACA AGTTGGAGAT GAAGAAACAT CATTGGATGA TGAAGAGGAA 
GATGGATTTT TGAAGGCGTT TAAGGTTGCA AATTTTGAGT ATATTGACGA AGCAGAGGCT 
GCCGCAGAGG AGGAAGCTCA AAAGGCAGCC ATGGAAACGA GATCCACCAT AAACAATTCT 
GAAAGAACTG AAAAAACAAA CTTTTGGGAA GAGTTGCTAA AAGATAGTTA TGAAGTACAC 
AAAGTTGAGG AGTTTAATGC CTTGGGCAAA GGAAAGAGAA GCCGTAAGCA GATGGTGTCT 
GTTGAAGATG ATGACCTTGC TGGTCTAGAA GATGTAAGCT CTGATGGCGA GGATGACAAT 
TATGAAGCTG AGTTGACTGA TGGTGAAACA ACATCATCAG GAGTAGTTCA GACAGTAAGA 
AGGCCTTATA AGAAGAAAGC TCGAGTGGAT AATACAGAAC CAATTCCTCT GATGGAAGGT 
GAAGGAAGAT CATTCAGAGT ATTGGGTTTT AAACAAAATC AGAGGGCTGC CTTTGTACAA 
ATCTTGATGA GGTTTGGGGT TGGGGACTAT GACTGGAAGG AGTTTGCCTC TCGATTGAAA 
CAGAAGACAT ATGAAGAAGT AGAAAATTAT GGGAGATTAT TCTTGACTCA CATAGCGGAA 
GATCTAACTG ACTCACCAAA TTTTTCAGAC GGTGTTCCTA AAGAGGGTCT TAGAATACAA 
GACGTACTTG TTAGGATTGC TGTCCTGCTT TTGATAAGGG ATAAGGCGAG GTTTGCATCA 
GAGAACCCAG GTAGCGCCCT TTTCACAGAT GACATTATAC TGCGCTACCC AGGCCTGAAG 
AGTGGAAAAT TTTGGAAACA AGAGCACGAT TCATTGTTAC TTCATGCAGT ATTGAAGCAT 
GGGTATGGAA GATGGCAAGC TATTGTTGAT GACAAGGACT TGAAGGTTCA AGAGATAATC 
TGTAAAGAGT TGAATCTTCC TTGTATAAGA CTACCTGTCC TTGGGCAAGG AGTTGCTCAA 
GCACAAAATG GTTCTACTTC TAACATTGCT AATGCAGAAG CTCCTAGCAC CCAGGCACAG 
GCAAATGTTG CTGGAAATGA TGTAGCAGCT GATGTTGCTC AGGGGACAAT TGATGCTGCA 
AACCCAGCAC TGTCATATCG AGATTCATCT ATATTATTTC ATTTTAGAGA TATGCAGAGA 
CGACAGGTAG AGTTCATTAA GAAAAGAGTG CTGCTTTTAG AGAGAGGACT CAATGCGGAG 
TACCAGAAAA TTTACTTTGG TGGTGATATA AAACCAAATG AGATTACAAG TGAAGAGGCT 
GATGGTGAAA CAAAGGCTGC TGACAGCTCA AGTTTAGGCT CCATAGAGAT CAATGCTCAA 
ATGATTGACC AGTTGCCTCA GATGGAACCA ATTGGATCAG AAGAAATTTC AGCAGCTGCT 
TGTGATGACA ATCCTGATAG ATTGGCATTG GCTGAGCATT ATAACAAGAT GTGCACGGTG 
TTGGAGCAAA ATGTTCATGA AACAATTCAG ATATCCTTAA CCAACCACCC TGCAAGTCTC 
AAGTTGAGAC AGGGCCTTCA GCCATTAGAG ATGATTTTTG AACAGATGAA TCAAATTCTC 
TCTCCTTTAC AACAAAAATC TACTTCGGAA CAAGGTACTC TGGGACCGAA CAAACATGTG 
CAAGCTGAAT CACAGAGCAA TCAGGCTAAA CTTCATTCAC CATCTGACCA ACAGAAGGAG 
AATAACGACA ATGCTGCTGC TGTGGAAGAT GTTGAAATGA AAGAGGCAAC GACAGAGCCA 
AAATTGGAAG AAACCATTGC ATCTTCGGAT GAAGAAGCTC CACATTCTGC AGACCCAGTT 
ACGCCTCCAA AAGAACCTAT GTGTTCACCA GGGACATCTG AAAAGGATGT GCAAATGGTC 
GATACAAGTA ATGGTACTGA CACCAACACT GATGCTGTAT CAAATGAAAA TGAAACCACA 
GAGAAATCAA ATTCGGGGGT GATCGTTTTA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7468676 ( XM_002309629.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002309665.2 Populus trichocarpa GYMNOS family protein (POPTR_0006s27810g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa14654
Clone ID Related Accession (Same CDS sequence)	XM_002309629.3 , XM_002309629.2
Accession Version	XM_002309629.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	GYMNOS family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008472). On Dec 16, 2013 this sequence version replaced XM_002309629.1.

ATGAGTAGCT TAGTGGAGAG GCTACGTGTT AGATCAGAAA GGAGGCCAGT TTATAACTTG 
GATGAATCAG ACGATGATGA TTATGTTTCC GGGAAAGCTA AAAATCCCCA GGAGAAAATT 
GAAAGATTTG TCAGGGATGA CGCGAAGGAG GATTCCTGTC AAGCTTGTGG AGAAAGTGAG 
AATCTTCTGA ATTGTGAAAC CTGCACATAT GCTTACCATT CCAAGTGCCT GCTTCCACCA 
CTAAAAGCTC CTTTTCCTAG CAATTGGAGG TGCCCTGAAT GTGTTAGCCC TCTGAATGAT 
ATCGATAAGT TATTGGACTG TGAAATGCGC CCTACTGTTG CTGATGATAG CGATGCCTCT 
AAACTGGGCT CAAAGCAAAT TTTTGTAAAA CAGTATCTTG TGAAATGGAA AGGCTTATCT 
TATCTACATT GCACTTGGGT ACCTGAGAAA GAGTTTTTAA AAGCTTTCAA GTCAAATCCC 
CGTCTGAAGA CTAAAGTAAA CAACTTCAAT CGTCAGATGG CATCAAACAA CAACTCCGAG 
GATGAATTTG TTGCTATCCG ACCTGAATGG ACAACTGTTG ATCGGATTCT TGCTTGCAGG 
GGAGATGAGG ATGAGAAGGA ATATCTTGTG AAGTACAAGG AACTCCCTTA CGATGAATGT 
TATTGGGAAT TTGAATCAGA TGTATCTGCA TTTCAGCCTG AAATAGAAAA ATTTAATAAA 
ATTCAATCTA GGTCTCACAA ACCGAGCAAG CAAAAGAGCA GCCTTCAAGA TGCTACTGAT 
TCAAAGAAGA AATCAAAAGA ATTTCAACAG TGTGATCACA GTCCTGAATT TCTTTCTGGA 
GGCTCATTGC ATCCTTATCA GTTAGAAGGG CTGAACTTCT TACGTTTTTC TTGGTCCAAA 
CAAACACATG TAATACTTGC TGATGAGATG GGCCTTGGGA AAACTATACA GAGTATTGCA 
TTTTTAGCAT CTCTTCGCGA AGAGGGCATC TCTCCGTATC TGGTAGTAGC TCCACTTTCA 
ACATTGCGAA ACTGGGAACG TGAATTTGCA ACATGGGCAC CTCAAATGAA TGTTGTTATG 
TATGTTGGCT CCGCACAAGC TCGTGCTGTC ATAAGGGAAT ATGAGTTTTA CTATCCCAAA 
AATCACAAGA AGATCAAGAA AAAGAAATCT GGTCAAGTTG TTACTGAAAG CAAGCAAGAT 
AGAATAAAAT TTGATGTGCT TCTAACGTCT TATGAGATGA TTAACTTGGA CTCAACATCG 
CTGAAACCAA TAAAGTGGGA ATGCATGATT GTTGATGAAG GTCATCGTCT AAAAAACAAG 
GACTCTAAAT TGTTTCTCTC GCTGAAGCAG TACTGTAGTA ACCATCGTGT GCTTTTGACT 
GGAACCCCTC TTCAGAACAA TCTGGATGAA CTTTTCATGC TCATGCATTT CCTTGATGCT 
GGGAAGTTTG CAAGTTTAGA GGAATTCCAA GAGGAGTTTA AGGATATAAA TCAAGAGGAG 
CAGATTTTGA GACTTCATAA AATGTTGGCT CCCCATCTCC TCAGAAGGGT GAAAAAGGAT 
GTCATGAAAG AGCTACCTCC TAAGAAGGAA CTCATTCTAC GTATTGAACT AAGTAGCAAG 
CAGAAAGAAT ATTACAAGGC CATTCTGACT CGCAACTATC AAATACTAAC TCGACGTGGT 
GGTGCACAGA TTTCTCTTAT CAATGTGGTT ATGGAATTGC GCAAACTCTG TTGTCATCCT 
TATATGTTAG AAGGAGTGGA GCCGGATATT GAAGATACCA ATGAATCTTT CAGGCAGCTC 
CTTGAAACTT CTGGGAAATT GCAACTGTTG GACAAACTGA TGGTGAGACT TAAAGAACAA 
GGGCATAGAG TTCTCATTTA CTCACAATTT CAGCACATGC TGGACTTGCT AGAAGATTAC 
TGCACTCATA AGAAATGGAT GTATGAACGG ATAGATGGAA AGGTTGGTGG AGCTGAGAGA 
CAAGTGCGCA TAGATCGATT TAATGCCAAA AATTCTTCAA GATTTTGCTT TCTTCTCTCA 
ACTAGAGCCG GAGGACTGGG AATAAACCTT GCAACTGCTG ATACTGTTAT TATATATGAT 
AGTGATTGGA ATCCCCATGC TGATCTTCAA GCAATGGCTA GAGCACATCG ACTTGGGCAA 
ACCAACAAGG TATTGATTTA TAGGCTCATA ACCCGAGGAA CTATTGAGGA ACGGATGATG 
CAACTGACTA AGAAGAAAAT GGTTTTAGAG CACCTTGTTG TTGGGAGGCT GAAGGCACAG 
AATATTAATC AGGAAGAGTT AGATGACATC ATAAGATATG GTTCAAAGGA GCTTTTTGCT 
GATGAGAATG ATGAAGCAGG GAAATCACGT CAAATTCATT ATGATGATGC TGCTATTGAT 
CGATTACTTG ACCGTGAACA AGTTGGAGAT GAAGAAACAT CATTGGATGA TGAAGAGGAA 
GATGGATTTT TGAAGGCGTT TAAGGTTGCA AATTTTGAGT ATATTGACGA AGCAGAGGCT 
GCCGCAGAGG AGGAAGCTCA AAAGGCAGCC ATGGAAACGA GATCCACCAT AAACAATTCT 
GAAAGAACTG AAAAAACAAA CTTTTGGGAA GAGTTGCTAA AAGATAGTTA TGAAGTACAC 
AAAGTTGAGG AGTTTAATGC CTTGGGCAAA GGAAAGAGAA GCCGTAAGCA GATGGTGTCT 
GTTGAAGATG ATGACCTTGC TGGTCTAGAA GATGTAAGCT CTGATGGCGA GGATGACAAT 
TATGAAGCTG AGTTGACTGA TGGTGAAACA ACATCATCAG GAGTAGTTCA GACAGTAAGA 
AGGCCTTATA AGAAGAAAGC TCGAGTGGAT AATACAGAAC CAATTCCTCT GATGGAAGGT 
GAAGGAAGAT CATTCAGAGT ATTGGGTTTT AAACAAAATC AGAGGGCTGC CTTTGTACAA 
ATCTTGATGA GGTTTGGGGT TGGGGACTAT GACTGGAAGG AGTTTGCCTC TCGATTGAAA 
CAGAAGACAT ATGAAGAAGT AGAAAATTAT GGGAGATTAT TCTTGACTCA CATAGCGGAA 
GATCTAACTG ACTCACCAAA TTTTTCAGAC GGTGTTCCTA AAGAGGGTCT TAGAATACAA 
GACGTACTTG TTAGGATTGC TGTCCTGCTT TTGATAAGGG ATAAGGCGAG GTTTGCATCA 
GAGAACCCAG GTAGCGCCCT TTTCACAGAT GACATTATAC TGCGCTACCC AGGCCTGAAG 
AGTGGAAAAT TTTGGAAACA AGAGCACGAT TCATTGTTAC TTCATGCAGT ATTGAAGCAT 
GGGTATGGAA GATGGCAAGC TATTGTTGAT GACAAGGACT TGAAGGTTCA AGAGATAATC 
TGTAAAGAGT TGAATCTTCC TTGTATAAGA CTACCTGTCC TTGGGCAAGG AGTTGCTCAA 
GCACAAAATG GTTCTACTTC TAACATTGCT AATGCAGAAG CTCCTAGCAC CCAGGCACAG 
GCAAATGTTG CTGGAAATGA TGTAGCAGCT GATGTTGCTC AGGGGACAAT TGATGCTGCA 
AACCCAGCAC TGTCATATCG AGATTCATCT ATATTATTTC ATTTTAGAGA TATGCAGAGA 
CGACAGGTAG AGTTCATTAA GAAAAGAGTG CTGCTTTTAG AGAGAGGACT CAATGCGGAG 
TACCAGAAAA TTTACTTTGG TGGTGATATA AAACCAAATG AGATTACAAG TGAAGAGGCT 
GATGGTGAAA CAAAGGCTGC TGACAGCTCA AGTTTAGGCT CCATAGAGAT CAATGCTCAA 
ATGATTGACC AGTTGCCTCA GATGGAACCA ATTGGATCAG AAGAAATTTC AGCAGCTGCT 
TGTGATGACA ATCCTGATAG ATTGGCATTG GCTGAGCATT ATAACAAGAT GTGCACGGTG 
TTGGAGCAAA ATGTTCATGA AACAATTCAG ATATCCTTAA CCAACCACCC TGCAAGTCTC 
AAGTTGAGAC AGGGCCTTCA GCCATTAGAG ATGATTTTTG AACAGATGAA TCAAATTCTC 
TCTCCTTTAC AACAAAAATC TACTTCGGAA CAAGGTACTC TGGGACCGAA CAAACATGTG 
CAAGCTGAAT CACAGAGCAA TCAGGCTAAA CTTCATTCAC CATCTGACCA ACAGAAGGAG 
AATAACGACA ATGCTGCTGC TGTGGAAGAT GTTGAAATGA AAGAGGCAAC GACAGAGCCA 
AAATTGGAAG AAACCATTGC ATCTTCGGAT GAAGAAGCTC CACATTCTGC AGACCCAGTT 
ACGCCTCCAA AAGAACCTAT GTGTTCACCA GGGACATCTG AAAAGGATGT GCAAATGGTC 
GATACAAGTA ATGGTACTGA CACCAACACT GATGCTGTAT CAAATGAAAA TGAAACCACA 
GAGAAATCAA ATTCGGGGGT GATCGTTTTA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002309665.2
CDS	131..4546
Translation	MSSLVERLRVRSERRPVYNLDESDDDDYVSGKAKNPQEKIERFVRDDAKEDSCQACGESENLLNCETCTYAYHSKCLLPPLKAPFPSNWRCPECVSPLNDIDKLLDCEMRPTVADDSDASKLGSKQIFVKQYLVKWKGLSYLHCTWVPEKEFLKAFKSNPRLKTKVNNFNRQMASNNNSEDEFVAIRPEWTTVDRILACRGDEDEKEYLVKYKELPYDECYWEFESDVSAFQPEIEKFNKIQSRSHKPSKQKSSLQDATDSKKKSKEFQQCDHSPEFLSGGSLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLREEGISPYLVVAPLSTLRNWEREFATWAPQMNVVMYVGSAQARAVIREYEFYYPKNHKKIKKKKSGQVVTESKQDRIKFDVLLTSYEMINLDSTSLKPIKWECMIVDEGHRLKNKDSKLFLSLKQYCSNHRVLLTGTPLQNNLDELFMLMHFLDAGKFASLEEFQEEFKDINQEEQILRLHKMLAPHLLRRVKKDVMKELPPKKELILRIELSSKQKEYYKAILTRNYQILTRRGGAQISLINVVMELRKLCCHPYMLEGVEPDIEDTNESFRQLLETSGKLQLLDKLMVRLKEQGHRVLIYSQFQHMLDLLEDYCTHKKWMYERIDGKVGGAERQVRIDRFNAKNSSRFCFLLSTRAGGLGINLATADTVIIYDSDWNPHADLQAMARAHRLGQTNKVLIYRLITRGTIEERMMQLTKKKMVLEHLVVGRLKAQNINQEELDDIIRYGSKELFADENDEAGKSRQIHYDDAAIDRLLDREQVGDEETSLDDEEEDGFLKAFKVANFEYIDEAEAAAEEEAQKAAMETRSTINNSERTEKTNFWEELLKDSYEVHKVEEFNALGKGKRSRKQMVSVEDDDLAGLEDVSSDGEDDNYEAELTDGETTSSGVVQTVRRPYKKKARVDNTEPIPLMEGEGRSFRVLGFKQNQRAAFVQILMRFGVGDYDWKEFASRLKQKTYEEVENYGRLFLTHIAEDLTDSPNFSDGVPKEGLRIQDVLVRIAVLLLIRDKARFASENPGSALFTDDIILRYPGLKSGKFWKQEHDSLLLHAVLKHGYGRWQAIVDDKDLKVQEIICKELNLPCIRLPVLGQGVAQAQNGSTSNIANAEAPSTQAQANVAGNDVAADVAQGTIDAANPALSYRDSSILFHFRDMQRRQVEFIKKRVLLLERGLNAEYQKIYFGGDIKPNEITSEEADGETKAADSSSLGSIEINAQMIDQLPQMEPIGSEEISAAACDDNPDRLALAEHYNKMCTVLEQNVHETIQISLTNHPASLKLRQGLQPLEMIFEQMNQILSPLQQKSTSEQGTLGPNKHVQAESQSNQAKLHSPSDQQKENNDNAAAVEDVEMKEATTEPKLEETIASSDEEAPHSADPVTPPKEPMCSPGTSEKDVQMVDTSNGTDTNTDAVSNENETTEKSNSGVIVLD

Target ORF information:

RefSeq Version	XM_002309629.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa GYMNOS family protein (POPTR_0006s27810g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002309629.2

ORF Insert Sequence:

ATGAGTAGCT TAGTGGAGAG GCTACGTGTT AGATCAGAAA GGAGGCCAGT TTATAACTTG 
GATGAATCAG ACGATGATGA TTATGTTTCC GGGAAAGCTA AAAATCCCCA GGAGAAAATT 
GAAAGATTTG TCAGGGATGA CGCGAAGGAG GATTCCTGTC AAGCTTGTGG AGAAAGTGAG 
AATCTTCTGA ATTGTGAAAC CTGCACATAT GCTTACCATT CCAAGTGCCT GCTTCCACCA 
CTAAAAGCTC CTTTTCCTAG CAATTGGAGG TGCCCTGAAT GTGTTAGCCC TCTGAATGAT 
ATCGATAAGT TATTGGACTG TGAAATGCGC CCTACTGTTG CTGATGATAG CGATGCCTCT 
AAACTGGGCT CAAAGCAAAT TTTTGTAAAA CAGTATCTTG TGAAATGGAA AGGCTTATCT 
TATCTACATT GCACTTGGGT ACCTGAGAAA GAGTTTTTAA AAGCTTTCAA GTCAAATCCC 
CGTCTGAAGA CTAAAGTAAA CAACTTCAAT CGTCAGATGG CATCAAACAA CAACTCCGAG 
GATGAATTTG TTGCTATCCG ACCTGAATGG ACAACTGTTG ATCGGATTCT TGCTTGCAGG 
GGAGATGAGG ATGAGAAGGA ATATCTTGTG AAGTACAAGG AACTCCCTTA CGATGAATGT 
TATTGGGAAT TTGAATCAGA TGTATCTGCA TTTCAGCCTG AAATAGAAAA ATTTAATAAA 
ATTCAATCTA GGTCTCACAA ACCGAGCAAG CAAAAGAGCA GCCTTCAAGA TGCTACTGAT 
TCAAAGAAGA AATCAAAAGA ATTTCAACAG TGTGATCACA GTCCTGAATT TCTTTCTGGA 
GGCTCATTGC ATCCTTATCA GTTAGAAGGG CTGAACTTCT TACGTTTTTC TTGGTCCAAA 
CAAACACATG TAATACTTGC TGATGAGATG GGCCTTGGGA AAACTATACA GAGTATTGCA 
TTTTTAGCAT CTCTTCGCGA AGAGGGCATC TCTCCGTATC TGGTAGTAGC TCCACTTTCA 
ACATTGCGAA ACTGGGAACG TGAATTTGCA ACATGGGCAC CTCAAATGAA TGTTGTTATG 
TATGTTGGCT CCGCACAAGC TCGTGCTGTC ATAAGGGAAT ATGAGTTTTA CTATCCCAAA 
AATCACAAGA AGATCAAGAA AAAGAAATCT GGTCAAGTTG TTACTGAAAG CAAGCAAGAT 
AGAATAAAAT TTGATGTGCT TCTAACGTCT TATGAGATGA TTAACTTGGA CTCAACATCG 
CTGAAACCAA TAAAGTGGGA ATGCATGATT GTTGATGAAG GTCATCGTCT AAAAAACAAG 
GACTCTAAAT TGTTTCTCTC GCTGAAGCAG TACTGTAGTA ACCATCGTGT GCTTTTGACT 
GGAACCCCTC TTCAGAACAA TCTGGATGAA CTTTTCATGC TCATGCATTT CCTTGATGCT 
GGGAAGTTTG CAAGTTTAGA GGAATTCCAA GAGGAGTTTA AGGATATAAA TCAAGAGGAG 
CAGATTTTGA GACTTCATAA AATGTTGGCT CCCCATCTCC TCAGAAGGGT GAAAAAGGAT 
GTCATGAAAG AGCTACCTCC TAAGAAGGAA CTCATTCTAC GTATTGAACT AAGTAGCAAG 
CAGAAAGAAT ATTACAAGGC CATTCTGACT CGCAACTATC AAATACTAAC TCGACGTGGT 
GGTGCACAGA TTTCTCTTAT CAATGTGGTT ATGGAATTGC GCAAACTCTG TTGTCATCCT 
TATATGTTAG AAGGAGTGGA GCCGGATATT GAAGATACCA ATGAATCTTT CAGGCAGCTC 
CTTGAAACTT CTGGGAAATT GCAACTGTTG GACAAACTGA TGGTGAGACT TAAAGAACAA 
GGGCATAGAG TTCTCATTTA CTCACAATTT CAGCACATGC TGGACTTGCT AGAAGATTAC 
TGCACTCATA AGAAATGGAT GTATGAACGG ATAGATGGAA AGGTTGGTGG AGCTGAGAGA 
CAAGTGCGCA TAGATCGATT TAATGCCAAA AATTCTTCAA GATTTTGCTT TCTTCTCTCA 
ACTAGAGCCG GAGGACTGGG AATAAACCTT GCAACTGCTG ATACTGTTAT TATATATGAT 
AGTGATTGGA ATCCCCATGC TGATCTTCAA GCAATGGCTA GAGCACATCG ACTTGGGCAA 
ACCAACAAGG TATTGATTTA TAGGCTCATA ACCCGAGGAA CTATTGAGGA ACGGATGATG 
CAACTGACTA AGAAGAAAAT GGTTTTAGAG CACCTTGTTG TTGGGAGGCT GAAGGCACAG 
AATATTAATC AGGAAGAGTT AGATGACATC ATAAGATATG GTTCAAAGGA GCTTTTTGCT 
GATGAGAATG ATGAAGCAGG GAAATCACGT CAAATTCATT ATGATGATGC TGCTATTGAT 
CGATTACTTG ACCGTGAACA AGTTGGAGAT GAAGAAACAT CATTGGATGA TGAAGAGGAA 
GATGGATTTT TGAAGGCGTT TAAGGTTGCA AATTTTGAGT ATATTGACGA AGCAGAGGCT 
GCCGCAGAGG AGGAAGCTCA AAAGGCAGCC ATGGAAACGA GATCCACCAT AAACAATTCT 
GAAAGAACTG AAAAAACAAA CTTTTGGGAA GAGTTGCTAA AAGATAGTTA TGAAGTACAC 
AAAGTTGAGG AGTTTAATGC CTTGGGCAAA GGAAAGAGAA GCCGTAAGCA GATGGTGTCT 
GTTGAAGATG ATGACCTTGC TGGTCTAGAA GATGTAAGCT CTGATGGCGA GGATGACAAT 
TATGAAGCTG AGTTGACTGA TGGTGAAACA ACATCATCAG GAGTAGTTCA GACAGTAAGA 
AGGCCTTATA AGAAGAAAGC TCGAGTGGAT AATACAGAAC CAATTCCTCT GATGGAAGGT 
GAAGGAAGAT CATTCAGAGT ATTGGGTTTT AAACAAAATC AGAGGGCTGC CTTTGTACAA 
ATCTTGATGA GGTTTGGGGT TGGGGACTAT GACTGGAAGG AGTTTGCCTC TCGATTGAAA 
CAGAAGACAT ATGAAGAAGT AGAAAATTAT GGGAGATTAT TCTTGACTCA CATAGCGGAA 
GATCTAACTG ACTCACCAAA TTTTTCAGAC GGTGTTCCTA AAGAGGGTCT TAGAATACAA 
GACGTACTTG TTAGGATTGC TGTCCTGCTT TTGATAAGGG ATAAGGCGAG GTTTGCATCA 
GAGAACCCAG GTAGCGCCCT TTTCACAGAT GACATTATAC TGCGCTACCC AGGCCTGAAG 
AGTGGAAAAT TTTGGAAACA AGAGCACGAT TCATTGTTAC TTCATGCAGT ATTGAAGCAT 
GGGTATGGAA GATGGCAAGC TATTGTTGAT GACAAGGACT TGAAGGTTCA AGAGATAATC 
TGTAAAGAGT TGAATCTTCC TTGTATAAGA CTACCTGTCC TTGGGCAAGG AGTTGCTCAA 
GCACAAAATG GTTCTACTTC TAACATTGCT AATGCAGAAG CTCCTAGCAC CCAGGCACAG 
GCAAATGTTG CTGGAAATGA TGTAGCAGCT GATGTTGCTC AGGGGACAAT TGATGCTGCA 
AACCCAGCAC TGTCATATCG AGATTCATCT ATATTATTTC ATTTTAGAGA TATGCAGAGA 
CGACAGGTAG AGTTCATTAA GAAAAGAGTG CTGCTTTTAG AGAGAGGACT CAATGCGGAG 
TACCAGAAAA TTTACTTTGG TGGTGATATA AAACCAAATG AGATTACAAG TGAAGAGGCT 
GATGGTGAAA CAAAGGCTGC TGACAGCTCA AGTTTAGGCT CCATAGAGAT CAATGCTCAA 
ATGATTGACC AGTTGCCTCA GATGGAACCA ATTGGATCAG AAGAAATTTC AGCAGCTGCT 
TGTGATGACA ATCCTGATAG ATTGGCATTG GCTGAGCATT ATAACAAGAT GTGCACGGTG 
TTGGAGCAAA ATGTTCATGA AACAATTCAG ATATCCTTAA CCAACCACCC TGCAAGTCTC 
AAGTTGAGAC AGGGCCTTCA GCCATTAGAG ATGATTTTTG AACAGATGAA TCAAATTCTC 
TCTCCTTTAC AACAAAAATC TACTTCGGAA CAAGGTACTC TGGGACCGAA CAAACATGTG 
CAAGCTGAAT CACAGAGCAA TCAGGCTAAA CTTCATTCAC CATCTGACCA ACAGAAGGAG 
AATAACGACA ATGCTGCTGC TGTGGAAGAT GTTGAAATGA AAGAGGCAAC GACAGAGCCA 
AAATTGGAAG AAACCATTGC ATCTTCGGAT GAAGAAGCTC CACATTCTGC AGACCCAGTT 
ACGCCTCCAA AAGAACCTAT GTGTTCACCA GGGACATCTG AAAAGGATGT GCAAATGGTC 
GATACAAGTA ATGGTACTGA CACCAACACT GATGCTGTAT CAAATGAAAA TGAAACCACA 
GAGAAATCAA ATTCGGGGGT GATCGTTTTA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7468676 ( XM_024603305.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024459073.1 Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X1, mRNA.

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CloneID	OPa431574
Clone ID Related Accession (Same CDS sequence)	XM_024603305.1
Accession Version	XM_024603305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	CHD3-type chromatin-remodeling factor PICKLE isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGGT GCACGTGCCA TAAAATGAGT AGCTTAGTGG AGAGGCTACG TGTTAGATCA 
GAAAGGAGGC CAGTTTATAA CTTGGATGAA TCAGACGATG ATGATTATGT TTCCGGGAAA 
GCTAAAAATC CCCAGGAGAA AATTGAAAGA TTTGTCAGGG ATGACGCGAA GGAGGATTCC 
TGTCAAGCTT GTGGAGAAAG TGAGAATCTT CTGAATTGTG AAACCTGCAC ATATGCTTAC 
CATTCCAAGT GCCTGCTTCC ACCACTAAAA GCTCCTTTTC CTAGCAATTG GAGGTGCCCT 
GAATGTGTTA GCCCTCTGAA TGATATCGAT AAGTTATTGG ACTGTGAAAT GCGCCCTACT 
GTTGCTGATG ATAGCGATGC CTCTAAACTG GGCTCAAAGC AAATTTTTGT AAAACAGTAT 
CTTGTGAAAT GGAAAGGCTT ATCTTATCTA CATTGCACTT GGGTACCTGA GAAAGAGTTT 
TTAAAAGCTT TCAAGTCAAA TCCCCGTCTG AAGACTAAAG TAAACAACTT CAATCGTCAG 
ATGGCATCAA ACAACAACTC CGAGGATGAA TTTGTTGCTA TCCGACCTGA ATGGACAACT 
GTTGATCGGA TTCTTGCTTG CAGGGGAGAT GAGGATGAGA AGGAATATCT TGTGAAGTAC 
AAGGAACTCC CTTACGATGA ATGTTATTGG GAATTTGAAT CAGATGTATC TGCATTTCAG 
CCTGAAATAG AAAAATTTAA TAAAATTCAA TCTAGGTCTC ACAAACCGAG CAAGCAAAAG 
AGCAGCCTTC AAGATGCTAC TGATTCAAAG AAGAAATCAA AAGAATTTCA ACAGTGTGAT 
CACAGTCCTG AATTTCTTTC TGGAGGCTCA TTGCATCCTT ATCAGTTAGA AGGGCTGAAC 
TTCTTACGTT TTTCTTGGTC CAAACAAACA CATGTAATAC TTGCTGATGA GATGGGCCTT 
GGGAAAACTA TACAGAGTAT TGCATTTTTA GCATCTCTTC GCGAAGAGGG CATCTCTCCG 
TATCTGGTAG TAGCTCCACT TTCAACATTG CGAAACTGGG AACGTGAATT TGCAACATGG 
GCACCTCAAA TGAATGTTGT TATGTATGTT GGCTCCGCAC AAGCTCGTGC TGTCATAAGG 
GAATATGAGT TTTACTATCC CAAAAATCAC AAGAAGATCA AGAAAAAGAA ATCTGGTCAA 
GTTGTTACTG AAAGCAAGCA AGATAGAATA AAATTTGATG TGCTTCTAAC GTCTTATGAG 
ATGATTAACT TGGACTCAAC ATCGCTGAAA CCAATAAAGT GGGAATGCAT GATTGTTGAT 
GAAGGTCATC GTCTAAAAAA CAAGGACTCT AAATTGTTTC TCTCGCTGAA GCAGTACTGT 
AGTAACCATC GTGTGCTTTT GACTGGAACC CCTCTTCAGA ACAATCTGGA TGAACTTTTC 
ATGCTCATGC ATTTCCTTGA TGCTGGGAAG TTTGCAAGTT TAGAGGAATT CCAAGAGGAG 
TTTAAGGATA TAAATCAAGA GGAGCAGATT TTGAGACTTC ATAAAATGTT GGCTCCCCAT 
CTCCTCAGAA GGGTGAAAAA GGATGTCATG AAAGAGCTAC CTCCTAAGAA GGAACTCATT 
CTACGTATTG AACTAAGTAG CAAGCAGAAA GAATATTACA AGGCCATTCT GACTCGCAAC 
TATCAAATAC TAACTCGACG TGGTGGTGCA CAGATTTCTC TTATCAATGT GGTTATGGAA 
TTGCGCAAAC TCTGTTGTCA TCCTTATATG TTAGAAGGAG TGGAGCCGGA TATTGAAGAT 
ACCAATGAAT CTTTCAGGCA GCTCCTTGAA ACTTCTGGGA AATTGCAACT GTTGGACAAA 
CTGATGGTGA GACTTAAAGA ACAAGGGCAT AGAGTTCTCA TTTACTCACA ATTTCAGCAC 
ATGCTGGACT TGCTAGAAGA TTACTGCACT CATAAGAAAT GGATGTATGA ACGGATAGAT 
GGAAAGGTTG GTGGAGCTGA GAGACAAGTG CGCATAGATC GATTTAATGC CAAAAATTCT 
TCAAGATTTT GCTTTCTTCT CTCAACTAGA GCCGGAGGAC TGGGAATAAA CCTTGCAACT 
GCTGATACTG TTATTATATA TGATAGTGAT TGGAATCCCC ATGCTGATCT TCAAGCAATG 
GCTAGAGCAC ATCGACTTGG GCAAACCAAC AAGGTATTGA TTTATAGGCT CATAACCCGA 
GGAACTATTG AGGAACGGAT GATGCAACTG ACTAAGAAGA AAATGGTTTT AGAGCACCTT 
GTTGTTGGGA GGCTGAAGGC ACAGAATATT AATCAGGAAG AGTTAGATGA CATCATAAGA 
TATGGTTCAA AGGAGCTTTT TGCTGATGAG AATGATGAAG CAGGGAAATC ACGTCAAATT 
CATTATGATG ATGCTGCTAT TGATCGATTA CTTGACCGTG AACAAGTTGG AGATGAAGAA 
ACATCATTGG ATGATGAAGA GGAAGATGGA TTTTTGAAGG CGTTTAAGGT TGCAAATTTT 
GAGTATATTG ACGAAGCAGA GGCTGCCGCA GAGGAGGAAG CTCAAAAGGC AGCCATGGAA 
ACGAGATCCA CCATAAACAA TTCTGAAAGA ACTGAAAAAA CAAACTTTTG GGAAGAGTTG 
CTAAAAGATA GTTATGAAGT ACACAAAGTT GAGGAGTTTA ATGCCTTGGG CAAAGGAAAG 
AGAAGCCGTA AGCAGATGGT GTCTGTTGAA GATGATGACC TTGCTGGTCT AGAAGATGTA 
AGCTCTGATG GCGAGGATGA CAATTATGAA GCTGAGTTGA CTGATGGTGA AACAACATCA 
TCAGGAGTAG TTCAGACAGT AAGAAGGCCT TATAAGAAGA AAGCTCGAGT GGATAATACA 
GAACCAATTC CTCTGATGGA AGGTGAAGGA AGATCATTCA GAGTATTGGG TTTTAAACAA 
AATCAGAGGG CTGCCTTTGT ACAAATCTTG ATGAGGTTTG GGGTTGGGGA CTATGACTGG 
AAGGAGTTTG CCTCTCGATT GAAACAGAAG ACATATGAAG AAGTAGAAAA TTATGGGAGA 
TTATTCTTGA CTCACATAGC GGAAGATCTA ACTGACTCAC CAAATTTTTC AGACGGTGTT 
CCTAAAGAGG GTCTTAGAAT ACAAGACGTA CTTGTTAGGA TTGCTGTCCT GCTTTTGATA 
AGGGATAAGG CGAGGTTTGC ATCAGAGAAC CCAGGTAGCG CCCTTTTCAC AGATGACATT 
ATACTGCGCT ACCCAGGCCT GAAGAGTGGA AAATTTTGGA AACAAGAGCA CGATTCATTG 
TTACTTCATG CAGTATTGAA GCATGGGTAT GGAAGATGGC AAGCTATTGT TGATGACAAG 
GACTTGAAGG TTCAAGAGAT AATCTGTAAA GAGTTGAATC TTCCTTGTAT AAGACTACCT 
GTCCTTGGGC AAGGAGTTGC TCAAGCACAA AATGGTTCTA CTTCTAACAT TGCTAATGCA 
GAAGCTCCTA GCACCCAGGC ACAGGCAAAT GTTGCTGGAA ATGATGTAGC AGCTGATGTT 
GCTCAGGGGA CAATTGATGC TGCAAACCCA GCACTGTCAT ATCGAGATTC ATCTATATTA 
TTTCATTTTA GAGATATGCA GAGACGACAG GTAGAGTTCA TTAAGAAAAG AGTGCTGCTT 
TTAGAGAGAG GACTCAATGC GGAGTACCAG AAAATTTACT TTGGTGGTGA TATAAAACCA 
AATGAGATTA CAAGTGAAGA GGCTGATGGT GAAACAAAGG CTGCTGACAG CTCAAGTTTA 
GGCTCCATAG AGATCAATGC TCAAATGATT GACCAGTTGC CTCAGATGGA ACCAATTGGA 
TCAGAAGAAA TTTCAGCAGC TGCTTGTGAT GACAATCCTG ATAGATTGGC ATTGGCTGAG 
CATTATAACA AGATGTGCAC GGTGTTGGAG CAAAATGTTC ATGAAACAAT TCAGATATCC 
TTAACCAACC ACCCTGCAAG TCTCAAGTTG AGACAGGGCC TTCAGCCATT AGAGATGATT 
TTTGAACAGA TGAATCAAAT TCTCTCTCCT TTACAACAAA AATCTACTTC GGAACAAGGT 
ACTCTGGGAC CGAACAAACA TGTGCAAGCT GAATCACAGA GCAATCAGGC TAAACTTCAT 
TCACCATCTG ACCAACAGAA GGAGAATAAC GACAATGCTG CTGCTGTGGA AGATGTTGAA 
ATGAAAGAGG CAACGACAGA GCCAAAATTG GAAGAAACCA TTGCATCTTC GGATGAAGAA 
GCTCCACATT CTGCAGACCC AGTTACGCCT CCAAAAGAAC CTATGTGTTC ACCAGGGACA 
TCTGAAAAGG ATGTGCAAAT GGTCGATACA AGTAATGGTA CTGACACCAA CACTGATGCT 
GTATCAAATG AAAATGAAAC CACAGAGAAA TCAAATTCGG GGGTGATCGT TTTAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459073.1
CDS	197..4636
Translation	MNWCTCHKMSSLVERLRVRSERRPVYNLDESDDDDYVSGKAKNPQEKIERFVRDDAKEDSCQACGESENLLNCETCTYAYHSKCLLPPLKAPFPSNWRCPECVSPLNDIDKLLDCEMRPTVADDSDASKLGSKQIFVKQYLVKWKGLSYLHCTWVPEKEFLKAFKSNPRLKTKVNNFNRQMASNNNSEDEFVAIRPEWTTVDRILACRGDEDEKEYLVKYKELPYDECYWEFESDVSAFQPEIEKFNKIQSRSHKPSKQKSSLQDATDSKKKSKEFQQCDHSPEFLSGGSLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLREEGISPYLVVAPLSTLRNWEREFATWAPQMNVVMYVGSAQARAVIREYEFYYPKNHKKIKKKKSGQVVTESKQDRIKFDVLLTSYEMINLDSTSLKPIKWECMIVDEGHRLKNKDSKLFLSLKQYCSNHRVLLTGTPLQNNLDELFMLMHFLDAGKFASLEEFQEEFKDINQEEQILRLHKMLAPHLLRRVKKDVMKELPPKKELILRIELSSKQKEYYKAILTRNYQILTRRGGAQISLINVVMELRKLCCHPYMLEGVEPDIEDTNESFRQLLETSGKLQLLDKLMVRLKEQGHRVLIYSQFQHMLDLLEDYCTHKKWMYERIDGKVGGAERQVRIDRFNAKNSSRFCFLLSTRAGGLGINLATADTVIIYDSDWNPHADLQAMARAHRLGQTNKVLIYRLITRGTIEERMMQLTKKKMVLEHLVVGRLKAQNINQEELDDIIRYGSKELFADENDEAGKSRQIHYDDAAIDRLLDREQVGDEETSLDDEEEDGFLKAFKVANFEYIDEAEAAAEEEAQKAAMETRSTINNSERTEKTNFWEELLKDSYEVHKVEEFNALGKGKRSRKQMVSVEDDDLAGLEDVSSDGEDDNYEAELTDGETTSSGVVQTVRRPYKKKARVDNTEPIPLMEGEGRSFRVLGFKQNQRAAFVQILMRFGVGDYDWKEFASRLKQKTYEEVENYGRLFLTHIAEDLTDSPNFSDGVPKEGLRIQDVLVRIAVLLLIRDKARFASENPGSALFTDDIILRYPGLKSGKFWKQEHDSLLLHAVLKHGYGRWQAIVDDKDLKVQEIICKELNLPCIRLPVLGQGVAQAQNGSTSNIANAEAPSTQAQANVAGNDVAADVAQGTIDAANPALSYRDSSILFHFRDMQRRQVEFIKKRVLLLERGLNAEYQKIYFGGDIKPNEITSEEADGETKAADSSSLGSIEINAQMIDQLPQMEPIGSEEISAAACDDNPDRLALAEHYNKMCTVLEQNVHETIQISLTNHPASLKLRQGLQPLEMIFEQMNQILSPLQQKSTSEQGTLGPNKHVQAESQSNQAKLHSPSDQQKENNDNAAAVEDVEMKEATTEPKLEETIASSDEEAPHSADPVTPPKEPMCSPGTSEKDVQMVDTSNGTDTNTDAVSNENETTEKSNSGVIVLD

Target ORF information:

RefSeq Version	XM_024603305.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603305.1

ORF Insert Sequence:

ATGAATTGGT GCACGTGCCA TAAAATGAGT AGCTTAGTGG AGAGGCTACG TGTTAGATCA 
GAAAGGAGGC CAGTTTATAA CTTGGATGAA TCAGACGATG ATGATTATGT TTCCGGGAAA 
GCTAAAAATC CCCAGGAGAA AATTGAAAGA TTTGTCAGGG ATGACGCGAA GGAGGATTCC 
TGTCAAGCTT GTGGAGAAAG TGAGAATCTT CTGAATTGTG AAACCTGCAC ATATGCTTAC 
CATTCCAAGT GCCTGCTTCC ACCACTAAAA GCTCCTTTTC CTAGCAATTG GAGGTGCCCT 
GAATGTGTTA GCCCTCTGAA TGATATCGAT AAGTTATTGG ACTGTGAAAT GCGCCCTACT 
GTTGCTGATG ATAGCGATGC CTCTAAACTG GGCTCAAAGC AAATTTTTGT AAAACAGTAT 
CTTGTGAAAT GGAAAGGCTT ATCTTATCTA CATTGCACTT GGGTACCTGA GAAAGAGTTT 
TTAAAAGCTT TCAAGTCAAA TCCCCGTCTG AAGACTAAAG TAAACAACTT CAATCGTCAG 
ATGGCATCAA ACAACAACTC CGAGGATGAA TTTGTTGCTA TCCGACCTGA ATGGACAACT 
GTTGATCGGA TTCTTGCTTG CAGGGGAGAT GAGGATGAGA AGGAATATCT TGTGAAGTAC 
AAGGAACTCC CTTACGATGA ATGTTATTGG GAATTTGAAT CAGATGTATC TGCATTTCAG 
CCTGAAATAG AAAAATTTAA TAAAATTCAA TCTAGGTCTC ACAAACCGAG CAAGCAAAAG 
AGCAGCCTTC AAGATGCTAC TGATTCAAAG AAGAAATCAA AAGAATTTCA ACAGTGTGAT 
CACAGTCCTG AATTTCTTTC TGGAGGCTCA TTGCATCCTT ATCAGTTAGA AGGGCTGAAC 
TTCTTACGTT TTTCTTGGTC CAAACAAACA CATGTAATAC TTGCTGATGA GATGGGCCTT 
GGGAAAACTA TACAGAGTAT TGCATTTTTA GCATCTCTTC GCGAAGAGGG CATCTCTCCG 
TATCTGGTAG TAGCTCCACT TTCAACATTG CGAAACTGGG AACGTGAATT TGCAACATGG 
GCACCTCAAA TGAATGTTGT TATGTATGTT GGCTCCGCAC AAGCTCGTGC TGTCATAAGG 
GAATATGAGT TTTACTATCC CAAAAATCAC AAGAAGATCA AGAAAAAGAA ATCTGGTCAA 
GTTGTTACTG AAAGCAAGCA AGATAGAATA AAATTTGATG TGCTTCTAAC GTCTTATGAG 
ATGATTAACT TGGACTCAAC ATCGCTGAAA CCAATAAAGT GGGAATGCAT GATTGTTGAT 
GAAGGTCATC GTCTAAAAAA CAAGGACTCT AAATTGTTTC TCTCGCTGAA GCAGTACTGT 
AGTAACCATC GTGTGCTTTT GACTGGAACC CCTCTTCAGA ACAATCTGGA TGAACTTTTC 
ATGCTCATGC ATTTCCTTGA TGCTGGGAAG TTTGCAAGTT TAGAGGAATT CCAAGAGGAG 
TTTAAGGATA TAAATCAAGA GGAGCAGATT TTGAGACTTC ATAAAATGTT GGCTCCCCAT 
CTCCTCAGAA GGGTGAAAAA GGATGTCATG AAAGAGCTAC CTCCTAAGAA GGAACTCATT 
CTACGTATTG AACTAAGTAG CAAGCAGAAA GAATATTACA AGGCCATTCT GACTCGCAAC 
TATCAAATAC TAACTCGACG TGGTGGTGCA CAGATTTCTC TTATCAATGT GGTTATGGAA 
TTGCGCAAAC TCTGTTGTCA TCCTTATATG TTAGAAGGAG TGGAGCCGGA TATTGAAGAT 
ACCAATGAAT CTTTCAGGCA GCTCCTTGAA ACTTCTGGGA AATTGCAACT GTTGGACAAA 
CTGATGGTGA GACTTAAAGA ACAAGGGCAT AGAGTTCTCA TTTACTCACA ATTTCAGCAC 
ATGCTGGACT TGCTAGAAGA TTACTGCACT CATAAGAAAT GGATGTATGA ACGGATAGAT 
GGAAAGGTTG GTGGAGCTGA GAGACAAGTG CGCATAGATC GATTTAATGC CAAAAATTCT 
TCAAGATTTT GCTTTCTTCT CTCAACTAGA GCCGGAGGAC TGGGAATAAA CCTTGCAACT 
GCTGATACTG TTATTATATA TGATAGTGAT TGGAATCCCC ATGCTGATCT TCAAGCAATG 
GCTAGAGCAC ATCGACTTGG GCAAACCAAC AAGGTATTGA TTTATAGGCT CATAACCCGA 
GGAACTATTG AGGAACGGAT GATGCAACTG ACTAAGAAGA AAATGGTTTT AGAGCACCTT 
GTTGTTGGGA GGCTGAAGGC ACAGAATATT AATCAGGAAG AGTTAGATGA CATCATAAGA 
TATGGTTCAA AGGAGCTTTT TGCTGATGAG AATGATGAAG CAGGGAAATC ACGTCAAATT 
CATTATGATG ATGCTGCTAT TGATCGATTA CTTGACCGTG AACAAGTTGG AGATGAAGAA 
ACATCATTGG ATGATGAAGA GGAAGATGGA TTTTTGAAGG CGTTTAAGGT TGCAAATTTT 
GAGTATATTG ACGAAGCAGA GGCTGCCGCA GAGGAGGAAG CTCAAAAGGC AGCCATGGAA 
ACGAGATCCA CCATAAACAA TTCTGAAAGA ACTGAAAAAA CAAACTTTTG GGAAGAGTTG 
CTAAAAGATA GTTATGAAGT ACACAAAGTT GAGGAGTTTA ATGCCTTGGG CAAAGGAAAG 
AGAAGCCGTA AGCAGATGGT GTCTGTTGAA GATGATGACC TTGCTGGTCT AGAAGATGTA 
AGCTCTGATG GCGAGGATGA CAATTATGAA GCTGAGTTGA CTGATGGTGA AACAACATCA 
TCAGGAGTAG TTCAGACAGT AAGAAGGCCT TATAAGAAGA AAGCTCGAGT GGATAATACA 
GAACCAATTC CTCTGATGGA AGGTGAAGGA AGATCATTCA GAGTATTGGG TTTTAAACAA 
AATCAGAGGG CTGCCTTTGT ACAAATCTTG ATGAGGTTTG GGGTTGGGGA CTATGACTGG 
AAGGAGTTTG CCTCTCGATT GAAACAGAAG ACATATGAAG AAGTAGAAAA TTATGGGAGA 
TTATTCTTGA CTCACATAGC GGAAGATCTA ACTGACTCAC CAAATTTTTC AGACGGTGTT 
CCTAAAGAGG GTCTTAGAAT ACAAGACGTA CTTGTTAGGA TTGCTGTCCT GCTTTTGATA 
AGGGATAAGG CGAGGTTTGC ATCAGAGAAC CCAGGTAGCG CCCTTTTCAC AGATGACATT 
ATACTGCGCT ACCCAGGCCT GAAGAGTGGA AAATTTTGGA AACAAGAGCA CGATTCATTG 
TTACTTCATG CAGTATTGAA GCATGGGTAT GGAAGATGGC AAGCTATTGT TGATGACAAG 
GACTTGAAGG TTCAAGAGAT AATCTGTAAA GAGTTGAATC TTCCTTGTAT AAGACTACCT 
GTCCTTGGGC AAGGAGTTGC TCAAGCACAA AATGGTTCTA CTTCTAACAT TGCTAATGCA 
GAAGCTCCTA GCACCCAGGC ACAGGCAAAT GTTGCTGGAA ATGATGTAGC AGCTGATGTT 
GCTCAGGGGA CAATTGATGC TGCAAACCCA GCACTGTCAT ATCGAGATTC ATCTATATTA 
TTTCATTTTA GAGATATGCA GAGACGACAG GTAGAGTTCA TTAAGAAAAG AGTGCTGCTT 
TTAGAGAGAG GACTCAATGC GGAGTACCAG AAAATTTACT TTGGTGGTGA TATAAAACCA 
AATGAGATTA CAAGTGAAGA GGCTGATGGT GAAACAAAGG CTGCTGACAG CTCAAGTTTA 
GGCTCCATAG AGATCAATGC TCAAATGATT GACCAGTTGC CTCAGATGGA ACCAATTGGA 
TCAGAAGAAA TTTCAGCAGC TGCTTGTGAT GACAATCCTG ATAGATTGGC ATTGGCTGAG 
CATTATAACA AGATGTGCAC GGTGTTGGAG CAAAATGTTC ATGAAACAAT TCAGATATCC 
TTAACCAACC ACCCTGCAAG TCTCAAGTTG AGACAGGGCC TTCAGCCATT AGAGATGATT 
TTTGAACAGA TGAATCAAAT TCTCTCTCCT TTACAACAAA AATCTACTTC GGAACAAGGT 
ACTCTGGGAC CGAACAAACA TGTGCAAGCT GAATCACAGA GCAATCAGGC TAAACTTCAT 
TCACCATCTG ACCAACAGAA GGAGAATAAC GACAATGCTG CTGCTGTGGA AGATGTTGAA 
ATGAAAGAGG CAACGACAGA GCCAAAATTG GAAGAAACCA TTGCATCTTC GGATGAAGAA 
GCTCCACATT CTGCAGACCC AGTTACGCCT CCAAAAGAAC CTATGTGTTC ACCAGGGACA 
TCTGAAAAGG ATGTGCAAAT GGTCGATACA AGTAATGGTA CTGACACCAA CACTGATGCT 
GTATCAAATG AAAATGAAAC CACAGAGAAA TCAAATTCGG GGGTGATCGT TTTAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7468676 ( XM_024603306.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024459074.1 Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X2, mRNA.

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CloneID	OPa431575
Clone ID Related Accession (Same CDS sequence)	XM_024603306.1
Accession Version	XM_024603306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	CHD3-type chromatin-remodeling factor PICKLE isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGGT GCACGTGCCA TAAAATGAGT AGCTTAGTGG AGAGGCTACG TGTTAGATCA 
GAAAGGAGGC CAGTTTATAA CTTGGATGAA TCAGACGATG ATGATTATGT TTCCGGGAAA 
GCTAAAAATC CCCAGGAGAA AATTGAAAGA TTTGTCAGGG ATGACGCGAA GGAGGATTCC 
TGTCAAGCTT GTGGAGAAAG TGAGAATCTT CTGAATTGTG AAACCTGCAC ATATGCTTAC 
CATTCCAAGT GCCTGCTTCC ACCACTAAAA GCTCCTTTTC CTAGCAATTG GAGGTGCCCT 
GAATGTGTTA GCCCTCTGAA TGATATCGAT AAGTTATTGG ACTGTGAAAT GCGCCCTACT 
GTTGCTGATG ATAGCGATGC CTCTAAACTG GGCTCAAAGC AAATTTTTGT AAAACAGTAT 
CTTGTGAAAT GGAAAGGCTT ATCTTATCTA CATTGCACTT GGGTACCTGA GAAAGAGTTT 
TTAAAAGCTT TCAAGTCAAA TCCCCGTCTG AAGACTAAAG TAAACAACTT CAATCGTCAG 
ATGGCATCAA ACAACAACTC CGAGGATGAA TTTGTTGCTA TCCGACCTGA ATGGACAACT 
GTTGATCGGA TTCTTGCTTG CAGGGGAGAT GAGGATGAGA AGGAATATCT TGTGAAGTAC 
AAGGAACTCC CTTACGATGA ATGTTATTGG GAATTTGAAT CAGATGTATC TGCATTTCAG 
CCTGAAATAG AAAAATTTAA TAAAATTCAA TCTAGGTCTC ACAAACCGAG CAAGCAAAAG 
AGCAGCCTTC AAGATGCTAC TGATTCAAAG AAGAAATCAA AAGAATTTCA ACAGTGTGAT 
CACAGTCCTG AATTTCTTTC TGGAGGCTCA TTGCATCCTT ATCAGTTAGA AGGGCTGAAC 
TTCTTACGTT TTTCTTGGTC CAAACAAACA CATGTAATAC TTGCTGATGA GATGGGCCTT 
GGGAAAACTA TACAGAGTAT TGCATTTTTA GCATCTCTTC GCGAAGAGGG CATCTCTCCG 
TATCTGGTAG TAGCTCCACT TTCAACATTG CGAAACTGGG AACGTGAATT TGCAACATGG 
GCACCTCAAA TGAATGTTGT TATGTATGTT GGCTCCGCAC AAGCTCGTGC TGTCATAAGG 
GAATATGAGT TTTACTATCC CAAAAATCAC AAGAAGATCA AGAAAAAGAA ATCTGGTCAA 
GTTGTTACTG AAAGCAAGCA AGATAGAATA AAATTTGATG TGCTTCTAAC GTCTTATGAG 
ATGATTAACT TGGACTCAAC ATCGCTGAAA CCAATAAAGT GGGAATGCAT GATTGTTGAT 
GAAGGTCATC GTCTAAAAAA CAAGGACTCT AAATTGTTTC TCTCGCTGAA GCAGTACTGT 
AGTAACCATC GTGTGCTTTT GACTGGAACC CCTCTTCAGA ACAATCTGGA TGAACTTTTC 
ATGCTCATGC ATTTCCTTGA TGCTGGGAAG TTTGCAAGTT TAGAGGAATT CCAAGAGGAG 
TTTAAGGATA TAAATCAAGA GGAGCAGATT TTGAGACTTC ATAAAATGTT GGCTCCCCAT 
CTCCTCAGAA GGGTGAAAAA GGATGTCATG AAAGAGCTAC CTCCTAAGAA GGAACTCATT 
CTACGTATTG AACTAAGTAG CAAGCAGAAA GAATATTACA AGGCCATTCT GACTCGCAAC 
TATCAAATAC TAACTCGACG TGGTGGTGCA CAGATTTCTC TTATCAATGT GGTTATGGAA 
TTGCGCAAAC TCTGTTGTCA TCCTTATATG TTAGAAGGAG TGGAGCCGGA TATTGAAGAT 
ACCAATGAAT CTTTCAGGCA GCTCCTTGAA ACTTCTGGGA AATTGCAACT GTTGGACAAA 
CTGATGGTGA GACTTAAAGA ACAAGGGCAT AGAGTTCTCA TTTACTCACA ATTTCAGCAC 
ATGCTGGACT TGCTAGAAGA TTACTGCACT CATAAGAAAT GGATGTATGA ACGGATAGAT 
GGAAAGGTTG GTGGAGCTGA GAGACAAGTG CGCATAGATC GATTTAATGC CAAAAATTCT 
TCAAGATTTT GCTTTCTTCT CTCAACTAGA GCCGGAGGAC TGGGAATAAA CCTTGCAACT 
GCTGATACTG TTATTATATA TGATAGTGAT TGGAATCCCC ATGCTGATCT TCAAGCAATG 
GCTAGAGCAC ATCGACTTGG GCAAACCAAC AAGGTATTGA TTTATAGGCT CATAACCCGA 
GGAACTATTG AGGAACGGAT GATGCAACTG ACTAAGAAGA AAATGGTTTT AGAGCACCTT 
GTTGTTGGGA GGCTGAAGGC ACAGAATATT AATCAGGAAG AGTTAGATGA CATCATAAGA 
TATGGTTCAA AGGAGCTTTT TGCTGATGAG AATGATGAAG CAGGGAAATC ACGTCAAATT 
CATTATGATG ATGCTGCTAT TGATCGATTA CTTGACCGTG AACAAGTTGG AGATGAAGAA 
ACATCATTGG ATGATGAAGA GGAAGATGGA TTTTTGAAGG CGTTTAAGGT TGCAAATTTT 
GAGTATATTG ACGAAGCAGA GGCTGCCGCA GAGGAGGAAG CTCAAAAGGC AGCCATGGAA 
ACGAGATCCA CCATAAACAA TTCTGAAAGA ACTGAAAAAA CAAACTTTTG GGAAGAGTTG 
CTAAAAGATA GTTATGAAGT ACACAAAGTT GAGGAGTTTA ATGCCTTGGG CAAAGGAAAG 
AGAAGCCGTA AGCAGATGGT GTCTGTTGAA GATGATGACC TTGCTGGTCT AGAAGATGTA 
AGCTCTGATG GCGAGGATGA CAATTATGAA GCTGAGTTGA CTGATGGTGA AACAACATCA 
TCAGGAGTAG TTCAGACAGT AAGAAGGCCT TATAAGAAGA AAGCTCGAGA ACCAATTCCT 
CTGATGGAAG GTGAAGGAAG ATCATTCAGA GTATTGGGTT TTAAACAAAA TCAGAGGGCT 
GCCTTTGTAC AAATCTTGAT GAGGTTTGGG GTTGGGGACT ATGACTGGAA GGAGTTTGCC 
TCTCGATTGA AACAGAAGAC ATATGAAGAA GTAGAAAATT ATGGGAGATT ATTCTTGACT 
CACATAGCGG AAGATCTAAC TGACTCACCA AATTTTTCAG ACGGTGTTCC TAAAGAGGGT 
CTTAGAATAC AAGACGTACT TGTTAGGATT GCTGTCCTGC TTTTGATAAG GGATAAGGCG 
AGGTTTGCAT CAGAGAACCC AGGTAGCGCC CTTTTCACAG ATGACATTAT ACTGCGCTAC 
CCAGGCCTGA AGAGTGGAAA ATTTTGGAAA CAAGAGCACG ATTCATTGTT ACTTCATGCA 
GTATTGAAGC ATGGGTATGG AAGATGGCAA GCTATTGTTG ATGACAAGGA CTTGAAGGTT 
CAAGAGATAA TCTGTAAAGA GTTGAATCTT CCTTGTATAA GACTACCTGT CCTTGGGCAA 
GGAGTTGCTC AAGCACAAAA TGGTTCTACT TCTAACATTG CTAATGCAGA AGCTCCTAGC 
ACCCAGGCAC AGGCAAATGT TGCTGGAAAT GATGTAGCAG CTGATGTTGC TCAGGGGACA 
ATTGATGCTG CAAACCCAGC ACTGTCATAT CGAGATTCAT CTATATTATT TCATTTTAGA 
GATATGCAGA GACGACAGGT AGAGTTCATT AAGAAAAGAG TGCTGCTTTT AGAGAGAGGA 
CTCAATGCGG AGTACCAGAA AATTTACTTT GGTGGTGATA TAAAACCAAA TGAGATTACA 
AGTGAAGAGG CTGATGGTGA AACAAAGGCT GCTGACAGCT CAAGTTTAGG CTCCATAGAG 
ATCAATGCTC AAATGATTGA CCAGTTGCCT CAGATGGAAC CAATTGGATC AGAAGAAATT 
TCAGCAGCTG CTTGTGATGA CAATCCTGAT AGATTGGCAT TGGCTGAGCA TTATAACAAG 
ATGTGCACGG TGTTGGAGCA AAATGTTCAT GAAACAATTC AGATATCCTT AACCAACCAC 
CCTGCAAGTC TCAAGTTGAG ACAGGGCCTT CAGCCATTAG AGATGATTTT TGAACAGATG 
AATCAAATTC TCTCTCCTTT ACAACAAAAA TCTACTTCGG AACAAGGTAC TCTGGGACCG 
AACAAACATG TGCAAGCTGA ATCACAGAGC AATCAGGCTA AACTTCATTC ACCATCTGAC 
CAACAGAAGG AGAATAACGA CAATGCTGCT GCTGTGGAAG ATGTTGAAAT GAAAGAGGCA 
ACGACAGAGC CAAAATTGGA AGAAACCATT GCATCTTCGG ATGAAGAAGC TCCACATTCT 
GCAGACCCAG TTACGCCTCC AAAAGAACCT ATGTGTTCAC CAGGGACATC TGAAAAGGAT 
GTGCAAATGG TCGATACAAG TAATGGTACT GACACCAACA CTGATGCTGT ATCAAATGAA 
AATGAAACCA CAGAGAAATC AAATTCGGGG GTGATCGTTT TAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459074.1
CDS	198..4625
Translation	MNWCTCHKMSSLVERLRVRSERRPVYNLDESDDDDYVSGKAKNPQEKIERFVRDDAKEDSCQACGESENLLNCETCTYAYHSKCLLPPLKAPFPSNWRCPECVSPLNDIDKLLDCEMRPTVADDSDASKLGSKQIFVKQYLVKWKGLSYLHCTWVPEKEFLKAFKSNPRLKTKVNNFNRQMASNNNSEDEFVAIRPEWTTVDRILACRGDEDEKEYLVKYKELPYDECYWEFESDVSAFQPEIEKFNKIQSRSHKPSKQKSSLQDATDSKKKSKEFQQCDHSPEFLSGGSLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLREEGISPYLVVAPLSTLRNWEREFATWAPQMNVVMYVGSAQARAVIREYEFYYPKNHKKIKKKKSGQVVTESKQDRIKFDVLLTSYEMINLDSTSLKPIKWECMIVDEGHRLKNKDSKLFLSLKQYCSNHRVLLTGTPLQNNLDELFMLMHFLDAGKFASLEEFQEEFKDINQEEQILRLHKMLAPHLLRRVKKDVMKELPPKKELILRIELSSKQKEYYKAILTRNYQILTRRGGAQISLINVVMELRKLCCHPYMLEGVEPDIEDTNESFRQLLETSGKLQLLDKLMVRLKEQGHRVLIYSQFQHMLDLLEDYCTHKKWMYERIDGKVGGAERQVRIDRFNAKNSSRFCFLLSTRAGGLGINLATADTVIIYDSDWNPHADLQAMARAHRLGQTNKVLIYRLITRGTIEERMMQLTKKKMVLEHLVVGRLKAQNINQEELDDIIRYGSKELFADENDEAGKSRQIHYDDAAIDRLLDREQVGDEETSLDDEEEDGFLKAFKVANFEYIDEAEAAAEEEAQKAAMETRSTINNSERTEKTNFWEELLKDSYEVHKVEEFNALGKGKRSRKQMVSVEDDDLAGLEDVSSDGEDDNYEAELTDGETTSSGVVQTVRRPYKKKAREPIPLMEGEGRSFRVLGFKQNQRAAFVQILMRFGVGDYDWKEFASRLKQKTYEEVENYGRLFLTHIAEDLTDSPNFSDGVPKEGLRIQDVLVRIAVLLLIRDKARFASENPGSALFTDDIILRYPGLKSGKFWKQEHDSLLLHAVLKHGYGRWQAIVDDKDLKVQEIICKELNLPCIRLPVLGQGVAQAQNGSTSNIANAEAPSTQAQANVAGNDVAADVAQGTIDAANPALSYRDSSILFHFRDMQRRQVEFIKKRVLLLERGLNAEYQKIYFGGDIKPNEITSEEADGETKAADSSSLGSIEINAQMIDQLPQMEPIGSEEISAAACDDNPDRLALAEHYNKMCTVLEQNVHETIQISLTNHPASLKLRQGLQPLEMIFEQMNQILSPLQQKSTSEQGTLGPNKHVQAESQSNQAKLHSPSDQQKENNDNAAAVEDVEMKEATTEPKLEETIASSDEEAPHSADPVTPPKEPMCSPGTSEKDVQMVDTSNGTDTNTDAVSNENETTEKSNSGVIVLD

Target ORF information:

RefSeq Version	XM_024603306.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603306.1

ORF Insert Sequence:

ATGAATTGGT GCACGTGCCA TAAAATGAGT AGCTTAGTGG AGAGGCTACG TGTTAGATCA 
GAAAGGAGGC CAGTTTATAA CTTGGATGAA TCAGACGATG ATGATTATGT TTCCGGGAAA 
GCTAAAAATC CCCAGGAGAA AATTGAAAGA TTTGTCAGGG ATGACGCGAA GGAGGATTCC 
TGTCAAGCTT GTGGAGAAAG TGAGAATCTT CTGAATTGTG AAACCTGCAC ATATGCTTAC 
CATTCCAAGT GCCTGCTTCC ACCACTAAAA GCTCCTTTTC CTAGCAATTG GAGGTGCCCT 
GAATGTGTTA GCCCTCTGAA TGATATCGAT AAGTTATTGG ACTGTGAAAT GCGCCCTACT 
GTTGCTGATG ATAGCGATGC CTCTAAACTG GGCTCAAAGC AAATTTTTGT AAAACAGTAT 
CTTGTGAAAT GGAAAGGCTT ATCTTATCTA CATTGCACTT GGGTACCTGA GAAAGAGTTT 
TTAAAAGCTT TCAAGTCAAA TCCCCGTCTG AAGACTAAAG TAAACAACTT CAATCGTCAG 
ATGGCATCAA ACAACAACTC CGAGGATGAA TTTGTTGCTA TCCGACCTGA ATGGACAACT 
GTTGATCGGA TTCTTGCTTG CAGGGGAGAT GAGGATGAGA AGGAATATCT TGTGAAGTAC 
AAGGAACTCC CTTACGATGA ATGTTATTGG GAATTTGAAT CAGATGTATC TGCATTTCAG 
CCTGAAATAG AAAAATTTAA TAAAATTCAA TCTAGGTCTC ACAAACCGAG CAAGCAAAAG 
AGCAGCCTTC AAGATGCTAC TGATTCAAAG AAGAAATCAA AAGAATTTCA ACAGTGTGAT 
CACAGTCCTG AATTTCTTTC TGGAGGCTCA TTGCATCCTT ATCAGTTAGA AGGGCTGAAC 
TTCTTACGTT TTTCTTGGTC CAAACAAACA CATGTAATAC TTGCTGATGA GATGGGCCTT 
GGGAAAACTA TACAGAGTAT TGCATTTTTA GCATCTCTTC GCGAAGAGGG CATCTCTCCG 
TATCTGGTAG TAGCTCCACT TTCAACATTG CGAAACTGGG AACGTGAATT TGCAACATGG 
GCACCTCAAA TGAATGTTGT TATGTATGTT GGCTCCGCAC AAGCTCGTGC TGTCATAAGG 
GAATATGAGT TTTACTATCC CAAAAATCAC AAGAAGATCA AGAAAAAGAA ATCTGGTCAA 
GTTGTTACTG AAAGCAAGCA AGATAGAATA AAATTTGATG TGCTTCTAAC GTCTTATGAG 
ATGATTAACT TGGACTCAAC ATCGCTGAAA CCAATAAAGT GGGAATGCAT GATTGTTGAT 
GAAGGTCATC GTCTAAAAAA CAAGGACTCT AAATTGTTTC TCTCGCTGAA GCAGTACTGT 
AGTAACCATC GTGTGCTTTT GACTGGAACC CCTCTTCAGA ACAATCTGGA TGAACTTTTC 
ATGCTCATGC ATTTCCTTGA TGCTGGGAAG TTTGCAAGTT TAGAGGAATT CCAAGAGGAG 
TTTAAGGATA TAAATCAAGA GGAGCAGATT TTGAGACTTC ATAAAATGTT GGCTCCCCAT 
CTCCTCAGAA GGGTGAAAAA GGATGTCATG AAAGAGCTAC CTCCTAAGAA GGAACTCATT 
CTACGTATTG AACTAAGTAG CAAGCAGAAA GAATATTACA AGGCCATTCT GACTCGCAAC 
TATCAAATAC TAACTCGACG TGGTGGTGCA CAGATTTCTC TTATCAATGT GGTTATGGAA 
TTGCGCAAAC TCTGTTGTCA TCCTTATATG TTAGAAGGAG TGGAGCCGGA TATTGAAGAT 
ACCAATGAAT CTTTCAGGCA GCTCCTTGAA ACTTCTGGGA AATTGCAACT GTTGGACAAA 
CTGATGGTGA GACTTAAAGA ACAAGGGCAT AGAGTTCTCA TTTACTCACA ATTTCAGCAC 
ATGCTGGACT TGCTAGAAGA TTACTGCACT CATAAGAAAT GGATGTATGA ACGGATAGAT 
GGAAAGGTTG GTGGAGCTGA GAGACAAGTG CGCATAGATC GATTTAATGC CAAAAATTCT 
TCAAGATTTT GCTTTCTTCT CTCAACTAGA GCCGGAGGAC TGGGAATAAA CCTTGCAACT 
GCTGATACTG TTATTATATA TGATAGTGAT TGGAATCCCC ATGCTGATCT TCAAGCAATG 
GCTAGAGCAC ATCGACTTGG GCAAACCAAC AAGGTATTGA TTTATAGGCT CATAACCCGA 
GGAACTATTG AGGAACGGAT GATGCAACTG ACTAAGAAGA AAATGGTTTT AGAGCACCTT 
GTTGTTGGGA GGCTGAAGGC ACAGAATATT AATCAGGAAG AGTTAGATGA CATCATAAGA 
TATGGTTCAA AGGAGCTTTT TGCTGATGAG AATGATGAAG CAGGGAAATC ACGTCAAATT 
CATTATGATG ATGCTGCTAT TGATCGATTA CTTGACCGTG AACAAGTTGG AGATGAAGAA 
ACATCATTGG ATGATGAAGA GGAAGATGGA TTTTTGAAGG CGTTTAAGGT TGCAAATTTT 
GAGTATATTG ACGAAGCAGA GGCTGCCGCA GAGGAGGAAG CTCAAAAGGC AGCCATGGAA 
ACGAGATCCA CCATAAACAA TTCTGAAAGA ACTGAAAAAA CAAACTTTTG GGAAGAGTTG 
CTAAAAGATA GTTATGAAGT ACACAAAGTT GAGGAGTTTA ATGCCTTGGG CAAAGGAAAG 
AGAAGCCGTA AGCAGATGGT GTCTGTTGAA GATGATGACC TTGCTGGTCT AGAAGATGTA 
AGCTCTGATG GCGAGGATGA CAATTATGAA GCTGAGTTGA CTGATGGTGA AACAACATCA 
TCAGGAGTAG TTCAGACAGT AAGAAGGCCT TATAAGAAGA AAGCTCGAGA ACCAATTCCT 
CTGATGGAAG GTGAAGGAAG ATCATTCAGA GTATTGGGTT TTAAACAAAA TCAGAGGGCT 
GCCTTTGTAC AAATCTTGAT GAGGTTTGGG GTTGGGGACT ATGACTGGAA GGAGTTTGCC 
TCTCGATTGA AACAGAAGAC ATATGAAGAA GTAGAAAATT ATGGGAGATT ATTCTTGACT 
CACATAGCGG AAGATCTAAC TGACTCACCA AATTTTTCAG ACGGTGTTCC TAAAGAGGGT 
CTTAGAATAC AAGACGTACT TGTTAGGATT GCTGTCCTGC TTTTGATAAG GGATAAGGCG 
AGGTTTGCAT CAGAGAACCC AGGTAGCGCC CTTTTCACAG ATGACATTAT ACTGCGCTAC 
CCAGGCCTGA AGAGTGGAAA ATTTTGGAAA CAAGAGCACG ATTCATTGTT ACTTCATGCA 
GTATTGAAGC ATGGGTATGG AAGATGGCAA GCTATTGTTG ATGACAAGGA CTTGAAGGTT 
CAAGAGATAA TCTGTAAAGA GTTGAATCTT CCTTGTATAA GACTACCTGT CCTTGGGCAA 
GGAGTTGCTC AAGCACAAAA TGGTTCTACT TCTAACATTG CTAATGCAGA AGCTCCTAGC 
ACCCAGGCAC AGGCAAATGT TGCTGGAAAT GATGTAGCAG CTGATGTTGC TCAGGGGACA 
ATTGATGCTG CAAACCCAGC ACTGTCATAT CGAGATTCAT CTATATTATT TCATTTTAGA 
GATATGCAGA GACGACAGGT AGAGTTCATT AAGAAAAGAG TGCTGCTTTT AGAGAGAGGA 
CTCAATGCGG AGTACCAGAA AATTTACTTT GGTGGTGATA TAAAACCAAA TGAGATTACA 
AGTGAAGAGG CTGATGGTGA AACAAAGGCT GCTGACAGCT CAAGTTTAGG CTCCATAGAG 
ATCAATGCTC AAATGATTGA CCAGTTGCCT CAGATGGAAC CAATTGGATC AGAAGAAATT 
TCAGCAGCTG CTTGTGATGA CAATCCTGAT AGATTGGCAT TGGCTGAGCA TTATAACAAG 
ATGTGCACGG TGTTGGAGCA AAATGTTCAT GAAACAATTC AGATATCCTT AACCAACCAC 
CCTGCAAGTC TCAAGTTGAG ACAGGGCCTT CAGCCATTAG AGATGATTTT TGAACAGATG 
AATCAAATTC TCTCTCCTTT ACAACAAAAA TCTACTTCGG AACAAGGTAC TCTGGGACCG 
AACAAACATG TGCAAGCTGA ATCACAGAGC AATCAGGCTA AACTTCATTC ACCATCTGAC 
CAACAGAAGG AGAATAACGA CAATGCTGCT GCTGTGGAAG ATGTTGAAAT GAAAGAGGCA 
ACGACAGAGC CAAAATTGGA AGAAACCATT GCATCTTCGG ATGAAGAAGC TCCACATTCT 
GCAGACCCAG TTACGCCTCC AAAAGAACCT ATGTGTTCAC CAGGGACATC TGAAAAGGAT 
GTGCAAATGG TCGATACAAG TAATGGTACT GACACCAACA CTGATGCTGT ATCAAATGAA 
AATGAAACCA CAGAGAAATC AAATTCGGGG GTGATCGTTT TAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7468676 ( XM_024603307.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024459075.1 Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X3, mRNA.

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CloneID	OPa431576
Clone ID Related Accession (Same CDS sequence)	XM_024603307.1
Accession Version	XM_024603307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	CHD3-type chromatin-remodeling factor PICKLE isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA GTAGCTTAGT GGAGAGGCTA CGTGTTAGAT CAGAAAGGAG GCCAGTTTAT 
AACTTGGATG AATCAGACGA TGATGATTAT GTTTCCGGGA AAGCTAAAAA TCCCCAGGAG 
AAAATTGAAA GATTTGTCAG GGATGACGCG AAGGAGGATT CCTGTCAAGC TTGTGGAGAA 
AGTGAGAATC TTCTGAATTG TGAAACCTGC ACATATGCTT ACCATTCCAA GTGCCTGCTT 
CCACCACTAA AAGCTCCTTT TCCTAGCAAT TGGAGGTGCC CTGAATGTGT TAGCCCTCTG 
AATGATATCG ATAAGTTATT GGACTGTGAA ATGCGCCCTA CTGTTGCTGA TGATAGCGAT 
GCCTCTAAAC TGGGCTCAAA GCAAATTTTT GTAAAACAGT ATCTTGTGAA ATGGAAAGGC 
TTATCTTATC TACATTGCAC TTGGGTACCT GAGAAAGAGT TTTTAAAAGC TTTCAAGTCA 
AATCCCCGTC TGAAGACTAA AGTAAACAAC TTCAATCGTC AGATGGCATC AAACAACAAC 
TCCGAGGATG AATTTGTTGC TATCCGACCT GAATGGACAA CTGTTGATCG GATTCTTGCT 
TGCAGGGGAG ATGAGGATGA GAAGGAATAT CTTGTGAAGT ACAAGGAACT CCCTTACGAT 
GAATGTTATT GGGAATTTGA ATCAGATGTA TCTGCATTTC AGCCTGAAAT AGAAAAATTT 
AATAAAATTC AATCTAGGTC TCACAAACCG AGCAAGCAAA AGAGCAGCCT TCAAGATGCT 
ACTGATTCAA AGAAGAAATC AAAAGAATTT CAACAGTGTG ATCACAGTCC TGAATTTCTT 
TCTGGAGGCT CATTGCATCC TTATCAGTTA GAAGGGCTGA ACTTCTTACG TTTTTCTTGG 
TCCAAACAAA CACATGTAAT ACTTGCTGAT GAGATGGGCC TTGGGAAAAC TATACAGAGT 
ATTGCATTTT TAGCATCTCT TCGCGAAGAG GGCATCTCTC CGTATCTGGT AGTAGCTCCA 
CTTTCAACAT TGCGAAACTG GGAACGTGAA TTTGCAACAT GGGCACCTCA AATGAATGTT 
GTTATGTATG TTGGCTCCGC ACAAGCTCGT GCTGTCATAA GGGAATATGA GTTTTACTAT 
CCCAAAAATC ACAAGAAGAT CAAGAAAAAG AAATCTGGTC AAGTTGTTAC TGAAAGCAAG 
CAAGATAGAA TAAAATTTGA TGTGCTTCTA ACGTCTTATG AGATGATTAA CTTGGACTCA 
ACATCGCTGA AACCAATAAA GTGGGAATGC ATGATTGTTG ATGAAGGTCA TCGTCTAAAA 
AACAAGGACT CTAAATTGTT TCTCTCGCTG AAGCAGTACT GTAGTAACCA TCGTGTGCTT 
TTGACTGGAA CCCCTCTTCA GAACAATCTG GATGAACTTT TCATGCTCAT GCATTTCCTT 
GATGCTGGGA AGTTTGCAAG TTTAGAGGAA TTCCAAGAGG AGTTTAAGGA TATAAATCAA 
GAGGAGCAGA TTTTGAGACT TCATAAAATG TTGGCTCCCC ATCTCCTCAG AAGGGTGAAA 
AAGGATGTCA TGAAAGAGCT ACCTCCTAAG AAGGAACTCA TTCTACGTAT TGAACTAAGT 
AGCAAGCAGA AAGAATATTA CAAGGCCATT CTGACTCGCA ACTATCAAAT ACTAACTCGA 
CGTGGTGGTG CACAGATTTC TCTTATCAAT GTGGTTATGG AATTGCGCAA ACTCTGTTGT 
CATCCTTATA TGTTAGAAGG AGTGGAGCCG GATATTGAAG ATACCAATGA ATCTTTCAGG 
CAGCTCCTTG AAACTTCTGG GAAATTGCAA CTGTTGGACA AACTGATGGT GAGACTTAAA 
GAACAAGGGC ATAGAGTTCT CATTTACTCA CAATTTCAGC ACATGCTGGA CTTGCTAGAA 
GATTACTGCA CTCATAAGAA ATGGATGTAT GAACGGATAG ATGGAAAGGT TGGTGGAGCT 
GAGAGACAAG TGCGCATAGA TCGATTTAAT GCCAAAAATT CTTCAAGATT TTGCTTTCTT 
CTCTCAACTA GAGCCGGAGG ACTGGGAATA AACCTTGCAA CTGCTGATAC TGTTATTATA 
TATGATAGTG ATTGGAATCC CCATGCTGAT CTTCAAGCAA TGGCTAGAGC ACATCGACTT 
GGGCAAACCA ACAAGGTATT GATTTATAGG CTCATAACCC GAGGAACTAT TGAGGAACGG 
ATGATGCAAC TGACTAAGAA GAAAATGGTT TTAGAGCACC TTGTTGTTGG GAGGCTGAAG 
GCACAGAATA TTAATCAGGA AGAGTTAGAT GACATCATAA GATATGGTTC AAAGGAGCTT 
TTTGCTGATG AGAATGATGA AGCAGGGAAA TCACGTCAAA TTCATTATGA TGATGCTGCT 
ATTGATCGAT TACTTGACCG TGAACAAGTT GGAGATGAAG AAACATCATT GGATGATGAA 
GAGGAAGATG GATTTTTGAA GGCGTTTAAG GTTGCAAATT TTGAGTATAT TGACGAAGCA 
GAGGCTGCCG CAGAGGAGGA AGCTCAAAAG GCAGCCATGG AAACGAGATC CACCATAAAC 
AATTCTGAAA GAACTGAAAA AACAAACTTT TGGGAAGAGT TGCTAAAAGA TAGTTATGAA 
GTACACAAAG TTGAGGAGTT TAATGCCTTG GGCAAAGGAA AGAGAAGCCG TAAGCAGATG 
GTGTCTGTTG AAGATGATGA CCTTGCTGGT CTAGAAGATG TAAGCTCTGA TGGCGAGGAT 
GACAATTATG AAGCTGAGTT GACTGATGGT GAAACAACAT CATCAGGAGT AGTTCAGACA 
GTAAGAAGGC CTTATAAGAA GAAAGCTCGA GTGGATAATA CAGAACCAAT TCCTCTGATG 
GAAGGTGAAG GAAGATCATT CAGAGTATTG GGTTTTAAAC AAAATCAGAG GGCTGCCTTT 
GTACAAATCT TGATGAGGTT TGGGGTTGGG GACTATGACT GGAAGGAGTT TGCCTCTCGA 
TTGAAACAGA AGACATATGA AGAAGTAGAA AATTATGGGA GATTATTCTT GACTCACATA 
GCGGAAGATC TAACTGACTC ACCAAATTTT TCAGACGGTG TTCCTAAAGA GGGTCTTAGA 
ATACAAGACG TACTTGTTAG GATTGCTGTC CTGCTTTTGA TAAGGGATAA GGCGAGGTTT 
GCATCAGAGA ACCCAGGTAG CGCCCTTTTC ACAGATGACA TTATACTGCG CTACCCAGGC 
CTGAAGAGTG GAAAATTTTG GAAACAAGAG CACGATTCAT TGTTACTTCA TGCAGTATTG 
AAGCATGGGT ATGGAAGATG GCAAGCTATT GTTGATGACA AGGACTTGAA GGTTCAAGAG 
ATAATCTGTA AAGAGTTGAA TCTTCCTTGT ATAAGACTAC CTGTCCTTGG GCAAGGAGTT 
GCTCAAGCAC AAAATGGTTC TACTTCTAAC ATTGCTAATG CAGAAGCTCC TAGCACCCAG 
GCACAGGCAA ATGTTGCTGG AAATGATGTA GCAGCTGATG TTGCTCAGGG GACAATTGAT 
GCTGCAAACC CAGCACTGTC ATATCGAGAT TCATCTATAT TATTTCATTT TAGAGATATG 
CAGAGACGAC AGGTAGAGTT CATTAAGAAA AGAGTGCTGC TTTTAGAGAG AGGACTCAAT 
GCGGAGTACC AGAAAATTTA CTTTGGTGGT GATATAAAAC CAAATGAGAT TACAAGTGAA 
GAGGCTGATG GTGAAACAAA GGCTGCTGAC AGCTCAAGTT TAGGCTCCAT AGAGATCAAT 
GCTCAAATGA TTGACCAGTT GCCTCAGATG GAACCAATTG GATCAGAAGA AATTTCAGCA 
GCTGCTTGTG ATGACAATCC TGATAGATTG GCATTGGCTG AGCATTATAA CAAGATGTGC 
ACGGTGTTGG AGCAAAATGT TCATGAAACA ATTCAGATAT CCTTAACCAA CCACCCTGCA 
AGTCTCAAGT TGAGACAGGG CCTTCAGCCA TTAGAGATGA TTTTTGAACA GATGAATCAA 
ATTCTCTCTC CTTTACAACA AAAATCTACT TCGGAACAAG GTACTCTGGG ACCGAACAAA 
CATGTGCAAG CTGAATCACA GAGCAATCAG GCTAAACTTC ATTCACCATC TGACCAACAG 
AAGGAGAATA ACGACAATGC TGCTGCTGTG GAAGATGTTG AAATGAAAGA GGCAACGACA 
GAGCCAAAAT TGGAAGAAAC CATTGCATCT TCGGATGAAG AAGCTCCACA TTCTGCAGAC 
CCAGTTACGC CTCCAAAAGA ACCTATGTGT TCACCAGGGA CATCTGAAAA GGATGTGCAA 
ATGGTCGATA CAAGTAATGG TACTGACACC AACACTGATG CTGTATCAAA TGAAAATGAA 
ACCACAGAGA AATCAAATTC GGGGGTGATC GTTTTAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024459075.1
CDS	149..4570
Translation	MKMSSLVERLRVRSERRPVYNLDESDDDDYVSGKAKNPQEKIERFVRDDAKEDSCQACGESENLLNCETCTYAYHSKCLLPPLKAPFPSNWRCPECVSPLNDIDKLLDCEMRPTVADDSDASKLGSKQIFVKQYLVKWKGLSYLHCTWVPEKEFLKAFKSNPRLKTKVNNFNRQMASNNNSEDEFVAIRPEWTTVDRILACRGDEDEKEYLVKYKELPYDECYWEFESDVSAFQPEIEKFNKIQSRSHKPSKQKSSLQDATDSKKKSKEFQQCDHSPEFLSGGSLHPYQLEGLNFLRFSWSKQTHVILADEMGLGKTIQSIAFLASLREEGISPYLVVAPLSTLRNWEREFATWAPQMNVVMYVGSAQARAVIREYEFYYPKNHKKIKKKKSGQVVTESKQDRIKFDVLLTSYEMINLDSTSLKPIKWECMIVDEGHRLKNKDSKLFLSLKQYCSNHRVLLTGTPLQNNLDELFMLMHFLDAGKFASLEEFQEEFKDINQEEQILRLHKMLAPHLLRRVKKDVMKELPPKKELILRIELSSKQKEYYKAILTRNYQILTRRGGAQISLINVVMELRKLCCHPYMLEGVEPDIEDTNESFRQLLETSGKLQLLDKLMVRLKEQGHRVLIYSQFQHMLDLLEDYCTHKKWMYERIDGKVGGAERQVRIDRFNAKNSSRFCFLLSTRAGGLGINLATADTVIIYDSDWNPHADLQAMARAHRLGQTNKVLIYRLITRGTIEERMMQLTKKKMVLEHLVVGRLKAQNINQEELDDIIRYGSKELFADENDEAGKSRQIHYDDAAIDRLLDREQVGDEETSLDDEEEDGFLKAFKVANFEYIDEAEAAAEEEAQKAAMETRSTINNSERTEKTNFWEELLKDSYEVHKVEEFNALGKGKRSRKQMVSVEDDDLAGLEDVSSDGEDDNYEAELTDGETTSSGVVQTVRRPYKKKARVDNTEPIPLMEGEGRSFRVLGFKQNQRAAFVQILMRFGVGDYDWKEFASRLKQKTYEEVENYGRLFLTHIAEDLTDSPNFSDGVPKEGLRIQDVLVRIAVLLLIRDKARFASENPGSALFTDDIILRYPGLKSGKFWKQEHDSLLLHAVLKHGYGRWQAIVDDKDLKVQEIICKELNLPCIRLPVLGQGVAQAQNGSTSNIANAEAPSTQAQANVAGNDVAADVAQGTIDAANPALSYRDSSILFHFRDMQRRQVEFIKKRVLLLERGLNAEYQKIYFGGDIKPNEITSEEADGETKAADSSSLGSIEINAQMIDQLPQMEPIGSEEISAAACDDNPDRLALAEHYNKMCTVLEQNVHETIQISLTNHPASLKLRQGLQPLEMIFEQMNQILSPLQQKSTSEQGTLGPNKHVQAESQSNQAKLHSPSDQQKENNDNAAAVEDVEMKEATTEPKLEETIASSDEEAPHSADPVTPPKEPMCSPGTSEKDVQMVDTSNGTDTNTDAVSNENETTEKSNSGVIVLD

Target ORF information:

RefSeq Version	XM_024603307.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa CHD3-type chromatin-remodeling factor PICKLE (LOC7468676), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024603307.1

ORF Insert Sequence:

ATGAAAATGA GTAGCTTAGT GGAGAGGCTA CGTGTTAGAT CAGAAAGGAG GCCAGTTTAT 
AACTTGGATG AATCAGACGA TGATGATTAT GTTTCCGGGA AAGCTAAAAA TCCCCAGGAG 
AAAATTGAAA GATTTGTCAG GGATGACGCG AAGGAGGATT CCTGTCAAGC TTGTGGAGAA 
AGTGAGAATC TTCTGAATTG TGAAACCTGC ACATATGCTT ACCATTCCAA GTGCCTGCTT 
CCACCACTAA AAGCTCCTTT TCCTAGCAAT TGGAGGTGCC CTGAATGTGT TAGCCCTCTG 
AATGATATCG ATAAGTTATT GGACTGTGAA ATGCGCCCTA CTGTTGCTGA TGATAGCGAT 
GCCTCTAAAC TGGGCTCAAA GCAAATTTTT GTAAAACAGT ATCTTGTGAA ATGGAAAGGC 
TTATCTTATC TACATTGCAC TTGGGTACCT GAGAAAGAGT TTTTAAAAGC TTTCAAGTCA 
AATCCCCGTC TGAAGACTAA AGTAAACAAC TTCAATCGTC AGATGGCATC AAACAACAAC 
TCCGAGGATG AATTTGTTGC TATCCGACCT GAATGGACAA CTGTTGATCG GATTCTTGCT 
TGCAGGGGAG ATGAGGATGA GAAGGAATAT CTTGTGAAGT ACAAGGAACT CCCTTACGAT 
GAATGTTATT GGGAATTTGA ATCAGATGTA TCTGCATTTC AGCCTGAAAT AGAAAAATTT 
AATAAAATTC AATCTAGGTC TCACAAACCG AGCAAGCAAA AGAGCAGCCT TCAAGATGCT 
ACTGATTCAA AGAAGAAATC AAAAGAATTT CAACAGTGTG ATCACAGTCC TGAATTTCTT 
TCTGGAGGCT CATTGCATCC TTATCAGTTA GAAGGGCTGA ACTTCTTACG TTTTTCTTGG 
TCCAAACAAA CACATGTAAT ACTTGCTGAT GAGATGGGCC TTGGGAAAAC TATACAGAGT 
ATTGCATTTT TAGCATCTCT TCGCGAAGAG GGCATCTCTC CGTATCTGGT AGTAGCTCCA 
CTTTCAACAT TGCGAAACTG GGAACGTGAA TTTGCAACAT GGGCACCTCA AATGAATGTT 
GTTATGTATG TTGGCTCCGC ACAAGCTCGT GCTGTCATAA GGGAATATGA GTTTTACTAT 
CCCAAAAATC ACAAGAAGAT CAAGAAAAAG AAATCTGGTC AAGTTGTTAC TGAAAGCAAG 
CAAGATAGAA TAAAATTTGA TGTGCTTCTA ACGTCTTATG AGATGATTAA CTTGGACTCA 
ACATCGCTGA AACCAATAAA GTGGGAATGC ATGATTGTTG ATGAAGGTCA TCGTCTAAAA 
AACAAGGACT CTAAATTGTT TCTCTCGCTG AAGCAGTACT GTAGTAACCA TCGTGTGCTT 
TTGACTGGAA CCCCTCTTCA GAACAATCTG GATGAACTTT TCATGCTCAT GCATTTCCTT 
GATGCTGGGA AGTTTGCAAG TTTAGAGGAA TTCCAAGAGG AGTTTAAGGA TATAAATCAA 
GAGGAGCAGA TTTTGAGACT TCATAAAATG TTGGCTCCCC ATCTCCTCAG AAGGGTGAAA 
AAGGATGTCA TGAAAGAGCT ACCTCCTAAG AAGGAACTCA TTCTACGTAT TGAACTAAGT 
AGCAAGCAGA AAGAATATTA CAAGGCCATT CTGACTCGCA ACTATCAAAT ACTAACTCGA 
CGTGGTGGTG CACAGATTTC TCTTATCAAT GTGGTTATGG AATTGCGCAA ACTCTGTTGT 
CATCCTTATA TGTTAGAAGG AGTGGAGCCG GATATTGAAG ATACCAATGA ATCTTTCAGG 
CAGCTCCTTG AAACTTCTGG GAAATTGCAA CTGTTGGACA AACTGATGGT GAGACTTAAA 
GAACAAGGGC ATAGAGTTCT CATTTACTCA CAATTTCAGC ACATGCTGGA CTTGCTAGAA 
GATTACTGCA CTCATAAGAA ATGGATGTAT GAACGGATAG ATGGAAAGGT TGGTGGAGCT 
GAGAGACAAG TGCGCATAGA TCGATTTAAT GCCAAAAATT CTTCAAGATT TTGCTTTCTT 
CTCTCAACTA GAGCCGGAGG ACTGGGAATA AACCTTGCAA CTGCTGATAC TGTTATTATA 
TATGATAGTG ATTGGAATCC CCATGCTGAT CTTCAAGCAA TGGCTAGAGC ACATCGACTT 
GGGCAAACCA ACAAGGTATT GATTTATAGG CTCATAACCC GAGGAACTAT TGAGGAACGG 
ATGATGCAAC TGACTAAGAA GAAAATGGTT TTAGAGCACC TTGTTGTTGG GAGGCTGAAG 
GCACAGAATA TTAATCAGGA AGAGTTAGAT GACATCATAA GATATGGTTC AAAGGAGCTT 
TTTGCTGATG AGAATGATGA AGCAGGGAAA TCACGTCAAA TTCATTATGA TGATGCTGCT 
ATTGATCGAT TACTTGACCG TGAACAAGTT GGAGATGAAG AAACATCATT GGATGATGAA 
GAGGAAGATG GATTTTTGAA GGCGTTTAAG GTTGCAAATT TTGAGTATAT TGACGAAGCA 
GAGGCTGCCG CAGAGGAGGA AGCTCAAAAG GCAGCCATGG AAACGAGATC CACCATAAAC 
AATTCTGAAA GAACTGAAAA AACAAACTTT TGGGAAGAGT TGCTAAAAGA TAGTTATGAA 
GTACACAAAG TTGAGGAGTT TAATGCCTTG GGCAAAGGAA AGAGAAGCCG TAAGCAGATG 
GTGTCTGTTG AAGATGATGA CCTTGCTGGT CTAGAAGATG TAAGCTCTGA TGGCGAGGAT 
GACAATTATG AAGCTGAGTT GACTGATGGT GAAACAACAT CATCAGGAGT AGTTCAGACA 
GTAAGAAGGC CTTATAAGAA GAAAGCTCGA GTGGATAATA CAGAACCAAT TCCTCTGATG 
GAAGGTGAAG GAAGATCATT CAGAGTATTG GGTTTTAAAC AAAATCAGAG GGCTGCCTTT 
GTACAAATCT TGATGAGGTT TGGGGTTGGG GACTATGACT GGAAGGAGTT TGCCTCTCGA 
TTGAAACAGA AGACATATGA AGAAGTAGAA AATTATGGGA GATTATTCTT GACTCACATA 
GCGGAAGATC TAACTGACTC ACCAAATTTT TCAGACGGTG TTCCTAAAGA GGGTCTTAGA 
ATACAAGACG TACTTGTTAG GATTGCTGTC CTGCTTTTGA TAAGGGATAA GGCGAGGTTT 
GCATCAGAGA ACCCAGGTAG CGCCCTTTTC ACAGATGACA TTATACTGCG CTACCCAGGC 
CTGAAGAGTG GAAAATTTTG GAAACAAGAG CACGATTCAT TGTTACTTCA TGCAGTATTG 
AAGCATGGGT ATGGAAGATG GCAAGCTATT GTTGATGACA AGGACTTGAA GGTTCAAGAG 
ATAATCTGTA AAGAGTTGAA TCTTCCTTGT ATAAGACTAC CTGTCCTTGG GCAAGGAGTT 
GCTCAAGCAC AAAATGGTTC TACTTCTAAC ATTGCTAATG CAGAAGCTCC TAGCACCCAG 
GCACAGGCAA ATGTTGCTGG AAATGATGTA GCAGCTGATG TTGCTCAGGG GACAATTGAT 
GCTGCAAACC CAGCACTGTC ATATCGAGAT TCATCTATAT TATTTCATTT TAGAGATATG 
CAGAGACGAC AGGTAGAGTT CATTAAGAAA AGAGTGCTGC TTTTAGAGAG AGGACTCAAT 
GCGGAGTACC AGAAAATTTA CTTTGGTGGT GATATAAAAC CAAATGAGAT TACAAGTGAA 
GAGGCTGATG GTGAAACAAA GGCTGCTGAC AGCTCAAGTT TAGGCTCCAT AGAGATCAAT 
GCTCAAATGA TTGACCAGTT GCCTCAGATG GAACCAATTG GATCAGAAGA AATTTCAGCA 
GCTGCTTGTG ATGACAATCC TGATAGATTG GCATTGGCTG AGCATTATAA CAAGATGTGC 
ACGGTGTTGG AGCAAAATGT TCATGAAACA ATTCAGATAT CCTTAACCAA CCACCCTGCA 
AGTCTCAAGT TGAGACAGGG CCTTCAGCCA TTAGAGATGA TTTTTGAACA GATGAATCAA 
ATTCTCTCTC CTTTACAACA AAAATCTACT TCGGAACAAG GTACTCTGGG ACCGAACAAA 
CATGTGCAAG CTGAATCACA GAGCAATCAG GCTAAACTTC ATTCACCATC TGACCAACAG 
AAGGAGAATA ACGACAATGC TGCTGCTGTG GAAGATGTTG AAATGAAAGA GGCAACGACA 
GAGCCAAAAT TGGAAGAAAC CATTGCATCT TCGGATGAAG AAGCTCCACA TTCTGCAGAC 
CCAGTTACGC CTCCAAAAGA ACCTATGTGT TCACCAGGGA CATCTGAAAA GGATGTGCAA 
ATGGTCGATA CAAGTAATGG TACTGACACC AACACTGATG CTGTATCAAA TGAAAATGAA 
ACCACAGAGA AATCAAATTC GGGGGTGATC GTTTTAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7468676 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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ORF Insert Sequence:

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