LOC7458970 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7458970
Entrez Gene ID	7458970
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_825684
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024590572.1	XP_024446340.1	protein EMBRYONIC FLOWER 1 isoform X1
XM_024590573.1	XP_024446341.1	protein EMBRYONIC FLOWER 1 isoform X2
XM_024590574.1	XP_024446342.1	protein EMBRYONIC FLOWER 1 isoform X3
XM_002324772.2	XP_002324808.2	hypothetical protein
XM_024590570.1	XP_024446338.1	protein EMBRYONIC FLOWER 1 isoform X1
XM_024590571.1	XP_024446339.1	protein EMBRYONIC FLOWER 1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7458970 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7458970 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa35344	XM_002324772.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0018s00580g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa448042	XM_024590571.1 Latest version!	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa448042	XM_024590570.1 Latest version!	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa448042	XM_024590572.1 Latest version!	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa448043	XM_024590573.1 Latest version!	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa448044	XM_024590574.1 Latest version!	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7458970 ( XM_002324772.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002324808.2 Populus trichocarpa hypothetical protein (POPTR_0018s00580g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa35344
Clone ID Related Accession (Same CDS sequence)	XM_002324772.2
Accession Version	XM_002324772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008484). On Dec 16, 2013 this sequence version replaced XM_002324772.1.

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGGAAAC ATATGGGGTC AGAAGATAAT 
TTGAAGCAGG AAAATCATAG ACTGGCATGC GTAGTAACTG AAGTTGTTTC AAGCCCAATT 
CAGAAGGCAG ATCATTCAGA TGAAATAGGC TGTGGACATC ATGAAGTTGC TGATGCTGAG 
TTTTCTAAAA ACCTTAATTG CATGGTAAAT AATGCTACTG GACTTTGTGA AGCAGGAAAA 
GAAACTTCCA TAGATGATCA ACACAAAGAG CTAATAGCTT GTGGTATATC TGGAGAAGTT 
GGTAACATTG ATGATGGGGC ACTCAGTACT GCCAACAAGG ATCCTGTGAG CCGCCCTTCC 
CTGGAATTAG ATGAGTATGA TGATCCTTCA TCTGAAAGTG CTGAGATCAT GGTTGGTCAT 
AATACTCAGG ATGTTCTTCA TGAAAATTCA AGTGGTTTGC ATCGTAGAAA AACTCGAAAG 
GTACGCTTGT TGACGGAATT GTTGTGTGAA AATGGGGATA GAGACACTGA TAACAGAACT 
CGGTTTTCTC TGCCCCATGC CTTTCCTGAT GCATCTGCTG GAGTTGATAA GATTTCTGTT 
CTCCAAGGGG AGGTGGCTAT CCAAGGCAAA GTTAGAAGGG GCTTGGGTCA TAATAGTAAA 
AGAAAGTTGC CTCAGGATGA AGATTCAAGA TCTCTGGAGA TGAGGTCTCC CAATAAAGTT 
TGCAAAGAAG TTGGGATCTT AAAGAGAGAT GGGGAATCTG CTGAACCCAT TGTGGCTTCT 
GAATCAGAAG AAGATGCATC TGGTAGAATG GGTTTACAAA CTGGCATGAA GATTCAGTGG 
GCCAAAAACA AAGTTGATAG AAGTCCCATT GTAAGTAAGA AGAAAAACAA AAAGGTACCA 
AGTTTTGATG AATGTTTGTC TCCAGACCCA TCCCAAGAAA ATTTGCCAAA TGAAATTGGG 
GAGAAAAATG GAGATAATTC TAGGCCCATT GCTGTTGATG GTGTACTGGC CAAGTCAGTA 
CACAATGCAT TTATAGGCAG AGAGATGGAC CTTTTCCCTT TGCCTGATCC AAGAATGGAG 
AAAAATGTTA GTGAGTATAA GAAGAAAGGA AAGATGCCTC AGTTTGATGA TTATCAGGTT 
TCCCCTACTC CTTGGAATCA TGACATTCTC AGAGAAGGTC CAGTGATTAG AAAAGATGTG 
GGGACAATAA ATACTGGACC TGTAGTTTTT CCATTTCATT CAGCACAGGA AACATCCCTT 
GAAAAGGGGC TGGATCTTTC TCTTAACAGC TACAAGACTG CACAAAGCTA TGATGGGAAA 
CATATTCCAC TGGTAGAAAA TAGGCGAAGT TGTTTATTTA CTTGGCAAGA AGGAACTTCC 
CAAATTCAGG CCAAGAGGAA AGCTTCAAAA ATTGAGCACG CGGGGAATAT CAGTTTCACT 
TCTAAAATTG CCCAAGATGC ACCTTTTGAG AAAGGACTAC ATTGTGATCC CAATACCAAG 
AGACCATCTT TTGAAATGCC CTTCCGCAGA GAGAAGCAGA AATACACCTC CCAGGTTGAA 
ATTGGGGGTT GCTCTCTCAT GCAGAAAAAG GACTTTTGTC ACAATAAAGG CAATGAGGGA 
ACCATTGGAA TACTGGAGCA TTCAGCTTTT CCAAGGAAAG ATGTCAACCA AAGAGCAGAC 
AAGGTGTGTG AACAGGGAGC TTTAGATGAC ATTCCAATGG AAATTGTAGA ACTCATGGCA 
AAGAATCAGT ATGAGAGGTG TCTTCCTGAT GGTGAATACG AGAAATGCCA GTTAGAAACA 
ACTAGTAGTT CAAGGAGGAG TCAGACAATA AATTTCAGTC AATTATTGGG GCTTGGAGGA 
TTAAGCTTAT TTCATCAGGA AACTACTCGG AAACAGAACC CTCCAGCTAG AAGAAATGAC 
ATAATAAAGA TTGGTGAAAT GGTAGGACAA ACTAAACAAA AGGAAGTGGA TTTCTTTTCT 
CAAGCTGATC GAAACCCATT CAGCATGAGG CAACCGGAAA AAATTCGTTC CCCTGTAGGG 
TTTGGGGCAT TTCTACAACT TCAAGAGAAG CCATCAGGCA GATTCCAACA TCCTGCTTCC 
AGTTATAACA TACAAAACAC TCCTCAAATT TGCAAACAGA GAGGGGAAGT GGTGGGGAAT 
AGGTCTTGTC ATACCAGGTT CCAGACCCCA GGAGCATGTA ACACATGCCA GAGCATTCCG 
CAGCAAAGTA AGGAAGCAAA TCAACTTTGG TCATCAATGA TGCCAAATCA CATGCCCTTT 
GTATATAGCA TTCCTCCTAA GTGTGTGACT CCATCTACCA ATGTTGATGT GTTTCCTCAT 
TCTCCTGGCA CTGTGCTCAA GGAAAATATG AATGGGGATC GTGTTCTTAA GTTTCCAAAC 
AAGAATGCTG CCAACCTTGG AAAACAAAAC AGGAATCTTG GCTCAGAAAC GCTCCTCAGG 
GCACATGCAG AATACCCTTT TGCTGGAAAA CATAATGGGA TTGAGCTTAA TCATAAGCCA 
ATGGGGTCCT TAGAACTGTA TTCTAATGAA ACAATACCAG CTATGCACTT ACTCAGCCTC 
ATGGACGCAG GAGTGCAGTC CAGTGCACCG ATCAATATGG ATGTAAACCC AAAGTTCCTC 
AAGAGACCTG CCATTATTCA CAATGCTGAA CCAAAGGAGT TTTCTAGGCT GGACACTGGG 
GCATATAAAG TCATTTCTTC TGTGAAGCAC CCACCACGTA ATCATAATGG TAAAAATCAA 
CTTGCAGAGA GTTCCCGTGA CCTTATTCCT ATCATGCAAA CAACAGCTGG TGCATCTTCT 
TTGTCAATTC GACATGATAA ACGTATTAGG AAGCCTGTTG ATCTTCCCAG TCCAGTTATT 
CAATATAAAG AGAGGAGAAA AGGCTCTGAT TCACGCACAC AGAATAAAGC AAATAGGTCA 
CAGACATCAG CAAATGGTCG TTTTGGCACC AACTGTGGAT CCATTCCAGC TCATAGCATG 
CGGATAATGT CTTTTGGTGC TCCAGATCCT TCAGTGTTTT CATTGCCATT CCGTGCATTG 
GAAAACCCAA ACAAGGACAA GTTGAAGTCC CTTGATAACA ATAGAATTGT TCACCCCCAC 
AAAAGCAGCT CTGAGACTGA AGTCTGCAGT GTGAACAGAA ACCCTGCTGA TTTCACCATA 
CCGGAAGCTG GAAATATGTA TATGATTGCA GGTGAAGCCC TTCGATTTGA AAAGGATGTT 
CCTTTTGCAA ATGGATCTCA CTCGCTTAAA TTGGATGGGC GCAAACGACA GAGGAAGCTT 
CCTGCTATGA AAGACCATGG ACGACCTCCA ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002324808.2
CDS	43..3741
Translation	MESTVLVEKNHPCSHSKLVSKVESSIRIDSITIDLDNVDEKIEAEKCSHFSMRGYVSEIRKRDWKMCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVWKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_002324772.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0018s00580g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002324772.2

ORF Insert Sequence:

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGGAAAC ATATGGGGTC AGAAGATAAT 
TTGAAGCAGG AAAATCATAG ACTGGCATGC GTAGTAACTG AAGTTGTTTC AAGCCCAATT 
CAGAAGGCAG ATCATTCAGA TGAAATAGGC TGTGGACATC ATGAAGTTGC TGATGCTGAG 
TTTTCTAAAA ACCTTAATTG CATGGTAAAT AATGCTACTG GACTTTGTGA AGCAGGAAAA 
GAAACTTCCA TAGATGATCA ACACAAAGAG CTAATAGCTT GTGGTATATC TGGAGAAGTT 
GGTAACATTG ATGATGGGGC ACTCAGTACT GCCAACAAGG ATCCTGTGAG CCGCCCTTCC 
CTGGAATTAG ATGAGTATGA TGATCCTTCA TCTGAAAGTG CTGAGATCAT GGTTGGTCAT 
AATACTCAGG ATGTTCTTCA TGAAAATTCA AGTGGTTTGC ATCGTAGAAA AACTCGAAAG 
GTACGCTTGT TGACGGAATT GTTGTGTGAA AATGGGGATA GAGACACTGA TAACAGAACT 
CGGTTTTCTC TGCCCCATGC CTTTCCTGAT GCATCTGCTG GAGTTGATAA GATTTCTGTT 
CTCCAAGGGG AGGTGGCTAT CCAAGGCAAA GTTAGAAGGG GCTTGGGTCA TAATAGTAAA 
AGAAAGTTGC CTCAGGATGA AGATTCAAGA TCTCTGGAGA TGAGGTCTCC CAATAAAGTT 
TGCAAAGAAG TTGGGATCTT AAAGAGAGAT GGGGAATCTG CTGAACCCAT TGTGGCTTCT 
GAATCAGAAG AAGATGCATC TGGTAGAATG GGTTTACAAA CTGGCATGAA GATTCAGTGG 
GCCAAAAACA AAGTTGATAG AAGTCCCATT GTAAGTAAGA AGAAAAACAA AAAGGTACCA 
AGTTTTGATG AATGTTTGTC TCCAGACCCA TCCCAAGAAA ATTTGCCAAA TGAAATTGGG 
GAGAAAAATG GAGATAATTC TAGGCCCATT GCTGTTGATG GTGTACTGGC CAAGTCAGTA 
CACAATGCAT TTATAGGCAG AGAGATGGAC CTTTTCCCTT TGCCTGATCC AAGAATGGAG 
AAAAATGTTA GTGAGTATAA GAAGAAAGGA AAGATGCCTC AGTTTGATGA TTATCAGGTT 
TCCCCTACTC CTTGGAATCA TGACATTCTC AGAGAAGGTC CAGTGATTAG AAAAGATGTG 
GGGACAATAA ATACTGGACC TGTAGTTTTT CCATTTCATT CAGCACAGGA AACATCCCTT 
GAAAAGGGGC TGGATCTTTC TCTTAACAGC TACAAGACTG CACAAAGCTA TGATGGGAAA 
CATATTCCAC TGGTAGAAAA TAGGCGAAGT TGTTTATTTA CTTGGCAAGA AGGAACTTCC 
CAAATTCAGG CCAAGAGGAA AGCTTCAAAA ATTGAGCACG CGGGGAATAT CAGTTTCACT 
TCTAAAATTG CCCAAGATGC ACCTTTTGAG AAAGGACTAC ATTGTGATCC CAATACCAAG 
AGACCATCTT TTGAAATGCC CTTCCGCAGA GAGAAGCAGA AATACACCTC CCAGGTTGAA 
ATTGGGGGTT GCTCTCTCAT GCAGAAAAAG GACTTTTGTC ACAATAAAGG CAATGAGGGA 
ACCATTGGAA TACTGGAGCA TTCAGCTTTT CCAAGGAAAG ATGTCAACCA AAGAGCAGAC 
AAGGTGTGTG AACAGGGAGC TTTAGATGAC ATTCCAATGG AAATTGTAGA ACTCATGGCA 
AAGAATCAGT ATGAGAGGTG TCTTCCTGAT GGTGAATACG AGAAATGCCA GTTAGAAACA 
ACTAGTAGTT CAAGGAGGAG TCAGACAATA AATTTCAGTC AATTATTGGG GCTTGGAGGA 
TTAAGCTTAT TTCATCAGGA AACTACTCGG AAACAGAACC CTCCAGCTAG AAGAAATGAC 
ATAATAAAGA TTGGTGAAAT GGTAGGACAA ACTAAACAAA AGGAAGTGGA TTTCTTTTCT 
CAAGCTGATC GAAACCCATT CAGCATGAGG CAACCGGAAA AAATTCGTTC CCCTGTAGGG 
TTTGGGGCAT TTCTACAACT TCAAGAGAAG CCATCAGGCA GATTCCAACA TCCTGCTTCC 
AGTTATAACA TACAAAACAC TCCTCAAATT TGCAAACAGA GAGGGGAAGT GGTGGGGAAT 
AGGTCTTGTC ATACCAGGTT CCAGACCCCA GGAGCATGTA ACACATGCCA GAGCATTCCG 
CAGCAAAGTA AGGAAGCAAA TCAACTTTGG TCATCAATGA TGCCAAATCA CATGCCCTTT 
GTATATAGCA TTCCTCCTAA GTGTGTGACT CCATCTACCA ATGTTGATGT GTTTCCTCAT 
TCTCCTGGCA CTGTGCTCAA GGAAAATATG AATGGGGATC GTGTTCTTAA GTTTCCAAAC 
AAGAATGCTG CCAACCTTGG AAAACAAAAC AGGAATCTTG GCTCAGAAAC GCTCCTCAGG 
GCACATGCAG AATACCCTTT TGCTGGAAAA CATAATGGGA TTGAGCTTAA TCATAAGCCA 
ATGGGGTCCT TAGAACTGTA TTCTAATGAA ACAATACCAG CTATGCACTT ACTCAGCCTC 
ATGGACGCAG GAGTGCAGTC CAGTGCACCG ATCAATATGG ATGTAAACCC AAAGTTCCTC 
AAGAGACCTG CCATTATTCA CAATGCTGAA CCAAAGGAGT TTTCTAGGCT GGACACTGGG 
GCATATAAAG TCATTTCTTC TGTGAAGCAC CCACCACGTA ATCATAATGG TAAAAATCAA 
CTTGCAGAGA GTTCCCGTGA CCTTATTCCT ATCATGCAAA CAACAGCTGG TGCATCTTCT 
TTGTCAATTC GACATGATAA ACGTATTAGG AAGCCTGTTG ATCTTCCCAG TCCAGTTATT 
CAATATAAAG AGAGGAGAAA AGGCTCTGAT TCACGCACAC AGAATAAAGC AAATAGGTCA 
CAGACATCAG CAAATGGTCG TTTTGGCACC AACTGTGGAT CCATTCCAGC TCATAGCATG 
CGGATAATGT CTTTTGGTGC TCCAGATCCT TCAGTGTTTT CATTGCCATT CCGTGCATTG 
GAAAACCCAA ACAAGGACAA GTTGAAGTCC CTTGATAACA ATAGAATTGT TCACCCCCAC 
AAAAGCAGCT CTGAGACTGA AGTCTGCAGT GTGAACAGAA ACCCTGCTGA TTTCACCATA 
CCGGAAGCTG GAAATATGTA TATGATTGCA GGTGAAGCCC TTCGATTTGA AAAGGATGTT 
CCTTTTGCAA ATGGATCTCA CTCGCTTAAA TTGGATGGGC GCAAACGACA GAGGAAGCTT 
CCTGCTATGA AAGACCATGG ACGACCTCCA ATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458970 ( XM_024590572.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446340.1 Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa448042
Clone ID Related Accession (Same CDS sequence)	XM_024590572.1 , XM_024590570.1 , XM_024590571.1
Accession Version	XM_024590572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	protein EMBRYONIC FLOWER 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446340.1
CDS	308..4237
Translation	MESTVLVEKNHPCSHSKLVSKVESSIRIDSITIDLDNVDEKIEAEKCSHFSMRGYVSEIRKRDWKMCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVCSHGPNFDDAARLPSDVQEATNQEIPEGRQADVFASLNSTSKSHHSLSIDKNEKKTKDMNGSIIGKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIVAFKSIGIDLCESGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_024590572.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590572.1

ORF Insert Sequence:

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458970 ( XM_024590570.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446338.1 Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X1, mRNA.

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CloneID	OPa448042
Clone ID Related Accession (Same CDS sequence)	XM_024590572.1 , XM_024590570.1 , XM_024590571.1
Accession Version	XM_024590570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	protein EMBRYONIC FLOWER 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446338.1
CDS	307..4236
Translation	MESTVLVEKNHPCSHSKLVSKVESSIRIDSITIDLDNVDEKIEAEKCSHFSMRGYVSEIRKRDWKMCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVCSHGPNFDDAARLPSDVQEATNQEIPEGRQADVFASLNSTSKSHHSLSIDKNEKKTKDMNGSIIGKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIVAFKSIGIDLCESGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_024590570.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590570.1

ORF Insert Sequence:

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458970 ( XM_024590571.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446339.1 Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa448042
Clone ID Related Accession (Same CDS sequence)	XM_024590572.1 , XM_024590570.1 , XM_024590571.1
Accession Version	XM_024590571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	protein EMBRYONIC FLOWER 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446339.1
CDS	339..4268
Translation	MESTVLVEKNHPCSHSKLVSKVESSIRIDSITIDLDNVDEKIEAEKCSHFSMRGYVSEIRKRDWKMCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVCSHGPNFDDAARLPSDVQEATNQEIPEGRQADVFASLNSTSKSHHSLSIDKNEKKTKDMNGSIIGKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIVAFKSIGIDLCESGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_024590571.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590571.1

ORF Insert Sequence:

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG TAGCTTTCAA GTCAATAGGT 
ATTGACTTAT GTGAGTCAGG CTGTGGACAT CATGAAGTTG CTGATGCTGA GTTTTCTAAA 
AACCTTAATT GCATGGTAAA TAATGCTACT GGACTTTGTG AAGCAGGAAA AGAAACTTCC 
ATAGATGATC AACACAAAGA GCTAATAGCT TGTGGTATAT CTGGAGAAGT TGGTAACATT 
GATGATGGGG CACTCAGTAC TGCCAACAAG GATCCTGTGA GCCGCCCTTC CCTGGAATTA 
GATGAGTATG ATGATCCTTC ATCTGAAAGT GCTGAGATCA TGGTTGGTCA TAATACTCAG 
GATGTTCTTC ATGAAAATTC AAGTGGTTTG CATCGTAGAA AAACTCGAAA GGTACGCTTG 
TTGACGGAAT TGTTGTGTGA AAATGGGGAT AGAGACACTG ATAACAGAAC TCGGTTTTCT 
CTGCCCCATG CCTTTCCTGA TGCATCTGCT GGAGTTGATA AGATTTCTGT TCTCCAAGGG 
GAGGTGGCTA TCCAAGGCAA AGTTAGAAGG GGCTTGGGTC ATAATAGTAA AAGAAAGTTG 
CCTCAGGATG AAGATTCAAG ATCTCTGGAG ATGAGGTCTC CCAATAAAGT TTGCAAAGAA 
GTTGGGATCT TAAAGAGAGA TGGGGAATCT GCTGAACCCA TTGTGGCTTC TGAATCAGAA 
GAAGATGCAT CTGGTAGAAT GGGTTTACAA ACTGGCATGA AGATTCAGTG GGCCAAAAAC 
AAAGTTGATA GAAGTCCCAT TGTAAGTAAG AAGAAAAACA AAAAGGTACC AAGTTTTGAT 
GAATGTTTGT CTCCAGACCC ATCCCAAGAA AATTTGCCAA ATGAAATTGG GGAGAAAAAT 
GGAGATAATT CTAGGCCCAT TGCTGTTGAT GGTGTACTGG CCAAGTCAGT ACACAATGCA 
TTTATAGGCA GAGAGATGGA CCTTTTCCCT TTGCCTGATC CAAGAATGGA GAAAAATGTT 
AGTGAGTATA AGAAGAAAGG AAAGATGCCT CAGTTTGATG ATTATCAGGT TTCCCCTACT 
CCTTGGAATC ATGACATTCT CAGAGAAGGT CCAGTGATTA GAAAAGATGT GGGGACAATA 
AATACTGGAC CTGTAGTTTT TCCATTTCAT TCAGCACAGG AAACATCCCT TGAAAAGGGG 
CTGGATCTTT CTCTTAACAG CTACAAGACT GCACAAAGCT ATGATGGGAA ACATATTCCA 
CTGGTAGAAA ATAGGCGAAG TTGTTTATTT ACTTGGCAAG AAGGAACTTC CCAAATTCAG 
GCCAAGAGGA AAGCTTCAAA AATTGAGCAC GCGGGGAATA TCAGTTTCAC TTCTAAAATT 
GCCCAAGATG CACCTTTTGA GAAAGGACTA CATTGTGATC CCAATACCAA GAGACCATCT 
TTTGAAATGC CCTTCCGCAG AGAGAAGCAG AAATACACCT CCCAGGTTGA AATTGGGGGT 
TGCTCTCTCA TGCAGAAAAA GGACTTTTGT CACAATAAAG GCAATGAGGG AACCATTGGA 
ATACTGGAGC ATTCAGCTTT TCCAAGGAAA GATGTCAACC AAAGAGCAGA CAAGGTGTGT 
GAACAGGGAG CTTTAGATGA CATTCCAATG GAAATTGTAG AACTCATGGC AAAGAATCAG 
TATGAGAGGT GTCTTCCTGA TGGTGAATAC GAGAAATGCC AGTTAGAAAC AACTAGTAGT 
TCAAGGAGGA GTCAGACAAT AAATTTCAGT CAATTATTGG GGCTTGGAGG ATTAAGCTTA 
TTTCATCAGG AAACTACTCG GAAACAGAAC CCTCCAGCTA GAAGAAATGA CATAATAAAG 
ATTGGTGAAA TGGTAGGACA AACTAAACAA AAGGAAGTGG ATTTCTTTTC TCAAGCTGAT 
CGAAACCCAT TCAGCATGAG GCAACCGGAA AAAATTCGTT CCCCTGTAGG GTTTGGGGCA 
TTTCTACAAC TTCAAGAGAA GCCATCAGGC AGATTCCAAC ATCCTGCTTC CAGTTATAAC 
ATACAAAACA CTCCTCAAAT TTGCAAACAG AGAGGGGAAG TGGTGGGGAA TAGGTCTTGT 
CATACCAGGT TCCAGACCCC AGGAGCATGT AACACATGCC AGAGCATTCC GCAGCAAAGT 
AAGGAAGCAA ATCAACTTTG GTCATCAATG ATGCCAAATC ACATGCCCTT TGTATATAGC 
ATTCCTCCTA AGTGTGTGAC TCCATCTACC AATGTTGATG TGTTTCCTCA TTCTCCTGGC 
ACTGTGCTCA AGGAAAATAT GAATGGGGAT CGTGTTCTTA AGTTTCCAAA CAAGAATGCT 
GCCAACCTTG GAAAACAAAA CAGGAATCTT GGCTCAGAAA CGCTCCTCAG GGCACATGCA 
GAATACCCTT TTGCTGGAAA ACATAATGGG ATTGAGCTTA ATCATAAGCC AATGGGGTCC 
TTGGAACTGT ATTCTAATGA AACAATACCA GCTATGCACT TACTCAGCCT CATGGACGCA 
GGAGTGCAGT CCAGTGCACC GATCAATATG GATGTAAACC CAAAGTTCCT CAAGAGACCT 
GCCATTATTC ACAATGCTGA ACCAAAGGAG TTTTCTAGGC TGGACACTGG GGCATATAAA 
GTCATTTCTT CTGTGAAGCA CCCACCACGT AATCATAATG GTAAAAATCA ACTTGCAGAG 
AGTTCCCGTG ACCTTATTCC TATCATGCAA ACAACAGCTG GTGCATCTTC TTTGTCAATT 
CGACATGATA AACGTATTAG GAAGCCTGTT GATCTTCCCA GTCCAGTTAT TCAATATAAA 
GAGAGGAGAA AAGGCTCTGA TTCACGCACA CAGAATAAAG CAAATAGGTC ACAGACATCA 
GCAAATGGTC GTTTTGGCAC CAACTGTGGA TCCATTCCAG CTCATAGCAT GCGGATAATG 
TCTTTTGGTG CTCCAGATCC TTCAGTGTTT TCATTGCCAT TCCGTGCATT GGAAAACCCA 
AACAAGGACA AGTTGAAGTC CCTTGATAAC AATAGAATTG TTCACCCCCA CAAAAGCAGC 
TCTGAGACTG AAGTCTGCAG TGTGAACAGA AACCCTGCTG ATTTCACCAT ACCGGAAGCT 
GGAAATATGT ATATGATTGC AGGTGAAGCC CTTCGATTTG AAAAGGATGT TCCTTTTGCA 
AATGGATCTC ACTCGCTTAA ATTGGATGGG CGCAAACGAC AGAGGAAGCT TCCTGCTATG 
AAAGACCATG GACGACCTCC AATGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458970 ( XM_024590573.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446341.1 Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa448043
Clone ID Related Accession (Same CDS sequence)	XM_024590573.1
Accession Version	XM_024590573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	protein EMBRYONIC FLOWER 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG CTTTCAAGTC AATAGGTATT 
GACTTATGTG AGTCAGGCTG TGGACATCAT GAAGTTGCTG ATGCTGAGTT TTCTAAAAAC 
CTTAATTGCA TGGTAAATAA TGCTACTGGA CTTTGTGAAG CAGGAAAAGA AACTTCCATA 
GATGATCAAC ACAAAGAGCT AATAGCTTGT GGTATATCTG GAGAAGTTGG TAACATTGAT 
GATGGGGCAC TCAGTACTGC CAACAAGGAT CCTGTGAGCC GCCCTTCCCT GGAATTAGAT 
GAGTATGATG ATCCTTCATC TGAAAGTGCT GAGATCATGG TTGGTCATAA TACTCAGGAT 
GTTCTTCATG AAAATTCAAG TGGTTTGCAT CGTAGAAAAA CTCGAAAGGT ACGCTTGTTG 
ACGGAATTGT TGTGTGAAAA TGGGGATAGA GACACTGATA ACAGAACTCG GTTTTCTCTG 
CCCCATGCCT TTCCTGATGC ATCTGCTGGA GTTGATAAGA TTTCTGTTCT CCAAGGGGAG 
GTGGCTATCC AAGGCAAAGT TAGAAGGGGC TTGGGTCATA ATAGTAAAAG AAAGTTGCCT 
CAGGATGAAG ATTCAAGATC TCTGGAGATG AGGTCTCCCA ATAAAGTTTG CAAAGAAGTT 
GGGATCTTAA AGAGAGATGG GGAATCTGCT GAACCCATTG TGGCTTCTGA ATCAGAAGAA 
GATGCATCTG GTAGAATGGG TTTACAAACT GGCATGAAGA TTCAGTGGGC CAAAAACAAA 
GTTGATAGAA GTCCCATTGT AAGTAAGAAG AAAAACAAAA AGGTACCAAG TTTTGATGAA 
TGTTTGTCTC CAGACCCATC CCAAGAAAAT TTGCCAAATG AAATTGGGGA GAAAAATGGA 
GATAATTCTA GGCCCATTGC TGTTGATGGT GTACTGGCCA AGTCAGTACA CAATGCATTT 
ATAGGCAGAG AGATGGACCT TTTCCCTTTG CCTGATCCAA GAATGGAGAA AAATGTTAGT 
GAGTATAAGA AGAAAGGAAA GATGCCTCAG TTTGATGATT ATCAGGTTTC CCCTACTCCT 
TGGAATCATG ACATTCTCAG AGAAGGTCCA GTGATTAGAA AAGATGTGGG GACAATAAAT 
ACTGGACCTG TAGTTTTTCC ATTTCATTCA GCACAGGAAA CATCCCTTGA AAAGGGGCTG 
GATCTTTCTC TTAACAGCTA CAAGACTGCA CAAAGCTATG ATGGGAAACA TATTCCACTG 
GTAGAAAATA GGCGAAGTTG TTTATTTACT TGGCAAGAAG GAACTTCCCA AATTCAGGCC 
AAGAGGAAAG CTTCAAAAAT TGAGCACGCG GGGAATATCA GTTTCACTTC TAAAATTGCC 
CAAGATGCAC CTTTTGAGAA AGGACTACAT TGTGATCCCA ATACCAAGAG ACCATCTTTT 
GAAATGCCCT TCCGCAGAGA GAAGCAGAAA TACACCTCCC AGGTTGAAAT TGGGGGTTGC 
TCTCTCATGC AGAAAAAGGA CTTTTGTCAC AATAAAGGCA ATGAGGGAAC CATTGGAATA 
CTGGAGCATT CAGCTTTTCC AAGGAAAGAT GTCAACCAAA GAGCAGACAA GGTGTGTGAA 
CAGGGAGCTT TAGATGACAT TCCAATGGAA ATTGTAGAAC TCATGGCAAA GAATCAGTAT 
GAGAGGTGTC TTCCTGATGG TGAATACGAG AAATGCCAGT TAGAAACAAC TAGTAGTTCA 
AGGAGGAGTC AGACAATAAA TTTCAGTCAA TTATTGGGGC TTGGAGGATT AAGCTTATTT 
CATCAGGAAA CTACTCGGAA ACAGAACCCT CCAGCTAGAA GAAATGACAT AATAAAGATT 
GGTGAAATGG TAGGACAAAC TAAACAAAAG GAAGTGGATT TCTTTTCTCA AGCTGATCGA 
AACCCATTCA GCATGAGGCA ACCGGAAAAA ATTCGTTCCC CTGTAGGGTT TGGGGCATTT 
CTACAACTTC AAGAGAAGCC ATCAGGCAGA TTCCAACATC CTGCTTCCAG TTATAACATA 
CAAAACACTC CTCAAATTTG CAAACAGAGA GGGGAAGTGG TGGGGAATAG GTCTTGTCAT 
ACCAGGTTCC AGACCCCAGG AGCATGTAAC ACATGCCAGA GCATTCCGCA GCAAAGTAAG 
GAAGCAAATC AACTTTGGTC ATCAATGATG CCAAATCACA TGCCCTTTGT ATATAGCATT 
CCTCCTAAGT GTGTGACTCC ATCTACCAAT GTTGATGTGT TTCCTCATTC TCCTGGCACT 
GTGCTCAAGG AAAATATGAA TGGGGATCGT GTTCTTAAGT TTCCAAACAA GAATGCTGCC 
AACCTTGGAA AACAAAACAG GAATCTTGGC TCAGAAACGC TCCTCAGGGC ACATGCAGAA 
TACCCTTTTG CTGGAAAACA TAATGGGATT GAGCTTAATC ATAAGCCAAT GGGGTCCTTG 
GAACTGTATT CTAATGAAAC AATACCAGCT ATGCACTTAC TCAGCCTCAT GGACGCAGGA 
GTGCAGTCCA GTGCACCGAT CAATATGGAT GTAAACCCAA AGTTCCTCAA GAGACCTGCC 
ATTATTCACA ATGCTGAACC AAAGGAGTTT TCTAGGCTGG ACACTGGGGC ATATAAAGTC 
ATTTCTTCTG TGAAGCACCC ACCACGTAAT CATAATGGTA AAAATCAACT TGCAGAGAGT 
TCCCGTGACC TTATTCCTAT CATGCAAACA ACAGCTGGTG CATCTTCTTT GTCAATTCGA 
CATGATAAAC GTATTAGGAA GCCTGTTGAT CTTCCCAGTC CAGTTATTCA ATATAAAGAG 
AGGAGAAAAG GCTCTGATTC ACGCACACAG AATAAAGCAA ATAGGTCACA GACATCAGCA 
AATGGTCGTT TTGGCACCAA CTGTGGATCC ATTCCAGCTC ATAGCATGCG GATAATGTCT 
TTTGGTGCTC CAGATCCTTC AGTGTTTTCA TTGCCATTCC GTGCATTGGA AAACCCAAAC 
AAGGACAAGT TGAAGTCCCT TGATAACAAT AGAATTGTTC ACCCCCACAA AAGCAGCTCT 
GAGACTGAAG TCTGCAGTGT GAACAGAAAC CCTGCTGATT TCACCATACC GGAAGCTGGA 
AATATGTATA TGATTGCAGG TGAAGCCCTT CGATTTGAAA AGGATGTTCC TTTTGCAAAT 
GGATCTCACT CGCTTAAATT GGATGGGCGC AAACGACAGA GGAAGCTTCC TGCTATGAAA 
GACCATGGAC GACCTCCAAT GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446341.1
CDS	308..4234
Translation	MESTVLVEKNHPCSHSKLVSKVESSIRIDSITIDLDNVDEKIEAEKCSHFSMRGYVSEIRKRDWKMCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVCSHGPNFDDAARLPSDVQEATNQEIPEGRQADVFASLNSTSKSHHSLSIDKNEKKTKDMNGSIIGKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIAFKSIGIDLCESGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_024590573.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590573.1

ORF Insert Sequence:

ATGGAAAGTA CTGTCTTAGT GGAGAAGAAT CATCCTTGTA GCCATTCAAA ACTTGTATCC 
AAGGTGGAAT CATCTATTAG GATTGATTCC ATAACCATCG ACCTGGACAA TGTTGATGAG 
AAAATTGAGG CAGAAAAATG CTCACACTTT TCTATGCGTG GATATGTATC TGAGATTCGT 
AAACGAGACT GGAAGATGTG TTGGCCGTTT GTGTCAGATG GTGACAGTAA TAACTATGAG 
GAGCAAGCAT GTCTGCTTCC TCCTTTACAT GTTCCGAAAT TCCGATTTTG GCGTTGTCAG 
AATTGTGTGT GGGAAGTTGG TGCCAAAGGC ACTGCAAACT GTTATGGAAC TGCTCTAAAA 
TCATGCGGAG CTGAACTCAA ATCCACCAAC GTTTGTTCCC ATGGACCAAA CTTTGATGAT 
GCTGCAAGAC TACCATCTGA TGTTCAGGAA GCCACAAATC AAGAAATTCC TGAGGGAAGA 
CAAGCTGATG TTTTTGCTAG TTTGAACAGT ACCAGTAAAT CTCATCATTC ACTGTCCATT 
GATAAAAATG AAAAGAAGAC TAAGGATATG AATGGTTCCA TCATAGGGAA ACATATGGGG 
TCAGAAGATA ATTTGAAGCA GGAAAATCAT AGACTGGCAT GCGTAGTAAC TGAAGTTGTT 
TCAAGCCCAA TTCAGAAGGC AGATCATTCA GATGAAATAG CTTTCAAGTC AATAGGTATT 
GACTTATGTG AGTCAGGCTG TGGACATCAT GAAGTTGCTG ATGCTGAGTT TTCTAAAAAC 
CTTAATTGCA TGGTAAATAA TGCTACTGGA CTTTGTGAAG CAGGAAAAGA AACTTCCATA 
GATGATCAAC ACAAAGAGCT AATAGCTTGT GGTATATCTG GAGAAGTTGG TAACATTGAT 
GATGGGGCAC TCAGTACTGC CAACAAGGAT CCTGTGAGCC GCCCTTCCCT GGAATTAGAT 
GAGTATGATG ATCCTTCATC TGAAAGTGCT GAGATCATGG TTGGTCATAA TACTCAGGAT 
GTTCTTCATG AAAATTCAAG TGGTTTGCAT CGTAGAAAAA CTCGAAAGGT ACGCTTGTTG 
ACGGAATTGT TGTGTGAAAA TGGGGATAGA GACACTGATA ACAGAACTCG GTTTTCTCTG 
CCCCATGCCT TTCCTGATGC ATCTGCTGGA GTTGATAAGA TTTCTGTTCT CCAAGGGGAG 
GTGGCTATCC AAGGCAAAGT TAGAAGGGGC TTGGGTCATA ATAGTAAAAG AAAGTTGCCT 
CAGGATGAAG ATTCAAGATC TCTGGAGATG AGGTCTCCCA ATAAAGTTTG CAAAGAAGTT 
GGGATCTTAA AGAGAGATGG GGAATCTGCT GAACCCATTG TGGCTTCTGA ATCAGAAGAA 
GATGCATCTG GTAGAATGGG TTTACAAACT GGCATGAAGA TTCAGTGGGC CAAAAACAAA 
GTTGATAGAA GTCCCATTGT AAGTAAGAAG AAAAACAAAA AGGTACCAAG TTTTGATGAA 
TGTTTGTCTC CAGACCCATC CCAAGAAAAT TTGCCAAATG AAATTGGGGA GAAAAATGGA 
GATAATTCTA GGCCCATTGC TGTTGATGGT GTACTGGCCA AGTCAGTACA CAATGCATTT 
ATAGGCAGAG AGATGGACCT TTTCCCTTTG CCTGATCCAA GAATGGAGAA AAATGTTAGT 
GAGTATAAGA AGAAAGGAAA GATGCCTCAG TTTGATGATT ATCAGGTTTC CCCTACTCCT 
TGGAATCATG ACATTCTCAG AGAAGGTCCA GTGATTAGAA AAGATGTGGG GACAATAAAT 
ACTGGACCTG TAGTTTTTCC ATTTCATTCA GCACAGGAAA CATCCCTTGA AAAGGGGCTG 
GATCTTTCTC TTAACAGCTA CAAGACTGCA CAAAGCTATG ATGGGAAACA TATTCCACTG 
GTAGAAAATA GGCGAAGTTG TTTATTTACT TGGCAAGAAG GAACTTCCCA AATTCAGGCC 
AAGAGGAAAG CTTCAAAAAT TGAGCACGCG GGGAATATCA GTTTCACTTC TAAAATTGCC 
CAAGATGCAC CTTTTGAGAA AGGACTACAT TGTGATCCCA ATACCAAGAG ACCATCTTTT 
GAAATGCCCT TCCGCAGAGA GAAGCAGAAA TACACCTCCC AGGTTGAAAT TGGGGGTTGC 
TCTCTCATGC AGAAAAAGGA CTTTTGTCAC AATAAAGGCA ATGAGGGAAC CATTGGAATA 
CTGGAGCATT CAGCTTTTCC AAGGAAAGAT GTCAACCAAA GAGCAGACAA GGTGTGTGAA 
CAGGGAGCTT TAGATGACAT TCCAATGGAA ATTGTAGAAC TCATGGCAAA GAATCAGTAT 
GAGAGGTGTC TTCCTGATGG TGAATACGAG AAATGCCAGT TAGAAACAAC TAGTAGTTCA 
AGGAGGAGTC AGACAATAAA TTTCAGTCAA TTATTGGGGC TTGGAGGATT AAGCTTATTT 
CATCAGGAAA CTACTCGGAA ACAGAACCCT CCAGCTAGAA GAAATGACAT AATAAAGATT 
GGTGAAATGG TAGGACAAAC TAAACAAAAG GAAGTGGATT TCTTTTCTCA AGCTGATCGA 
AACCCATTCA GCATGAGGCA ACCGGAAAAA ATTCGTTCCC CTGTAGGGTT TGGGGCATTT 
CTACAACTTC AAGAGAAGCC ATCAGGCAGA TTCCAACATC CTGCTTCCAG TTATAACATA 
CAAAACACTC CTCAAATTTG CAAACAGAGA GGGGAAGTGG TGGGGAATAG GTCTTGTCAT 
ACCAGGTTCC AGACCCCAGG AGCATGTAAC ACATGCCAGA GCATTCCGCA GCAAAGTAAG 
GAAGCAAATC AACTTTGGTC ATCAATGATG CCAAATCACA TGCCCTTTGT ATATAGCATT 
CCTCCTAAGT GTGTGACTCC ATCTACCAAT GTTGATGTGT TTCCTCATTC TCCTGGCACT 
GTGCTCAAGG AAAATATGAA TGGGGATCGT GTTCTTAAGT TTCCAAACAA GAATGCTGCC 
AACCTTGGAA AACAAAACAG GAATCTTGGC TCAGAAACGC TCCTCAGGGC ACATGCAGAA 
TACCCTTTTG CTGGAAAACA TAATGGGATT GAGCTTAATC ATAAGCCAAT GGGGTCCTTG 
GAACTGTATT CTAATGAAAC AATACCAGCT ATGCACTTAC TCAGCCTCAT GGACGCAGGA 
GTGCAGTCCA GTGCACCGAT CAATATGGAT GTAAACCCAA AGTTCCTCAA GAGACCTGCC 
ATTATTCACA ATGCTGAACC AAAGGAGTTT TCTAGGCTGG ACACTGGGGC ATATAAAGTC 
ATTTCTTCTG TGAAGCACCC ACCACGTAAT CATAATGGTA AAAATCAACT TGCAGAGAGT 
TCCCGTGACC TTATTCCTAT CATGCAAACA ACAGCTGGTG CATCTTCTTT GTCAATTCGA 
CATGATAAAC GTATTAGGAA GCCTGTTGAT CTTCCCAGTC CAGTTATTCA ATATAAAGAG 
AGGAGAAAAG GCTCTGATTC ACGCACACAG AATAAAGCAA ATAGGTCACA GACATCAGCA 
AATGGTCGTT TTGGCACCAA CTGTGGATCC ATTCCAGCTC ATAGCATGCG GATAATGTCT 
TTTGGTGCTC CAGATCCTTC AGTGTTTTCA TTGCCATTCC GTGCATTGGA AAACCCAAAC 
AAGGACAAGT TGAAGTCCCT TGATAACAAT AGAATTGTTC ACCCCCACAA AAGCAGCTCT 
GAGACTGAAG TCTGCAGTGT GAACAGAAAC CCTGCTGATT TCACCATACC GGAAGCTGGA 
AATATGTATA TGATTGCAGG TGAAGCCCTT CGATTTGAAA AGGATGTTCC TTTTGCAAAT 
GGATCTCACT CGCTTAAATT GGATGGGCGC AAACGACAGA GGAAGCTTCC TGCTATGAAA 
GACCATGGAC GACCTCCAAT GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7458970 ( XM_024590574.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024446342.1 Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X5, mRNA.

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CloneID	OPa448044
Clone ID Related Accession (Same CDS sequence)	XM_024590574.1
Accession Version	XM_024590574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	protein EMBRYONIC FLOWER 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGC CGTTTGTGTC AGATGGTGAC AGTAATAACT ATGAGGAGCA AGCATGTCTG 
CTTCCTCCTT TACATGTTCC GAAATTCCGA TTTTGGCGTT GTCAGAATTG TGTGTGGGAA 
GTTGGTGCCA AAGGCACTGC AAACTGTTAT GGAACTGCTC TAAAATCATG CGGAGCTGAA 
CTCAAATCCA CCAACGTTTG TTCCCATGGA CCAAACTTTG ATGATGCTGC AAGACTACCA 
TCTGATGTTC AGGAAGCCAC AAATCAAGAA ATTCCTGAGG GAAGACAAGC TGATGTTTTT 
GCTAGTTTGA ACAGTACCAG TAAATCTCAT CATTCACTGT CCATTGATAA AAATGAAAAG 
AAGACTAAGG ATATGAATGG TTCCATCATA GGGAAACATA TGGGGTCAGA AGATAATTTG 
AAGCAGGAAA ATCATAGACT GGCATGCGTA GTAACTGAAG TTGTTTCAAG CCCAATTCAG 
AAGGCAGATC ATTCAGATGA AATAGTAGCT TTCAAGTCAA TAGGTATTGA CTTATGTGAG 
TCAGGCTGTG GACATCATGA AGTTGCTGAT GCTGAGTTTT CTAAAAACCT TAATTGCATG 
GTAAATAATG CTACTGGACT TTGTGAAGCA GGAAAAGAAA CTTCCATAGA TGATCAACAC 
AAAGAGCTAA TAGCTTGTGG TATATCTGGA GAAGTTGGTA ACATTGATGA TGGGGCACTC 
AGTACTGCCA ACAAGGATCC TGTGAGCCGC CCTTCCCTGG AATTAGATGA GTATGATGAT 
CCTTCATCTG AAAGTGCTGA GATCATGGTT GGTCATAATA CTCAGGATGT TCTTCATGAA 
AATTCAAGTG GTTTGCATCG TAGAAAAACT CGAAAGGTAC GCTTGTTGAC GGAATTGTTG 
TGTGAAAATG GGGATAGAGA CACTGATAAC AGAACTCGGT TTTCTCTGCC CCATGCCTTT 
CCTGATGCAT CTGCTGGAGT TGATAAGATT TCTGTTCTCC AAGGGGAGGT GGCTATCCAA 
GGCAAAGTTA GAAGGGGCTT GGGTCATAAT AGTAAAAGAA AGTTGCCTCA GGATGAAGAT 
TCAAGATCTC TGGAGATGAG GTCTCCCAAT AAAGTTTGCA AAGAAGTTGG GATCTTAAAG 
AGAGATGGGG AATCTGCTGA ACCCATTGTG GCTTCTGAAT CAGAAGAAGA TGCATCTGGT 
AGAATGGGTT TACAAACTGG CATGAAGATT CAGTGGGCCA AAAACAAAGT TGATAGAAGT 
CCCATTGTAA GTAAGAAGAA AAACAAAAAG GTACCAAGTT TTGATGAATG TTTGTCTCCA 
GACCCATCCC AAGAAAATTT GCCAAATGAA ATTGGGGAGA AAAATGGAGA TAATTCTAGG 
CCCATTGCTG TTGATGGTGT ACTGGCCAAG TCAGTACACA ATGCATTTAT AGGCAGAGAG 
ATGGACCTTT TCCCTTTGCC TGATCCAAGA ATGGAGAAAA ATGTTAGTGA GTATAAGAAG 
AAAGGAAAGA TGCCTCAGTT TGATGATTAT CAGGTTTCCC CTACTCCTTG GAATCATGAC 
ATTCTCAGAG AAGGTCCAGT GATTAGAAAA GATGTGGGGA CAATAAATAC TGGACCTGTA 
GTTTTTCCAT TTCATTCAGC ACAGGAAACA TCCCTTGAAA AGGGGCTGGA TCTTTCTCTT 
AACAGCTACA AGACTGCACA AAGCTATGAT GGGAAACATA TTCCACTGGT AGAAAATAGG 
CGAAGTTGTT TATTTACTTG GCAAGAAGGA ACTTCCCAAA TTCAGGCCAA GAGGAAAGCT 
TCAAAAATTG AGCACGCGGG GAATATCAGT TTCACTTCTA AAATTGCCCA AGATGCACCT 
TTTGAGAAAG GACTACATTG TGATCCCAAT ACCAAGAGAC CATCTTTTGA AATGCCCTTC 
CGCAGAGAGA AGCAGAAATA CACCTCCCAG GTTGAAATTG GGGGTTGCTC TCTCATGCAG 
AAAAAGGACT TTTGTCACAA TAAAGGCAAT GAGGGAACCA TTGGAATACT GGAGCATTCA 
GCTTTTCCAA GGAAAGATGT CAACCAAAGA GCAGACAAGG TGTGTGAACA GGGAGCTTTA 
GATGACATTC CAATGGAAAT TGTAGAACTC ATGGCAAAGA ATCAGTATGA GAGGTGTCTT 
CCTGATGGTG AATACGAGAA ATGCCAGTTA GAAACAACTA GTAGTTCAAG GAGGAGTCAG 
ACAATAAATT TCAGTCAATT ATTGGGGCTT GGAGGATTAA GCTTATTTCA TCAGGAAACT 
ACTCGGAAAC AGAACCCTCC AGCTAGAAGA AATGACATAA TAAAGATTGG TGAAATGGTA 
GGACAAACTA AACAAAAGGA AGTGGATTTC TTTTCTCAAG CTGATCGAAA CCCATTCAGC 
ATGAGGCAAC CGGAAAAAAT TCGTTCCCCT GTAGGGTTTG GGGCATTTCT ACAACTTCAA 
GAGAAGCCAT CAGGCAGATT CCAACATCCT GCTTCCAGTT ATAACATACA AAACACTCCT 
CAAATTTGCA AACAGAGAGG GGAAGTGGTG GGGAATAGGT CTTGTCATAC CAGGTTCCAG 
ACCCCAGGAG CATGTAACAC ATGCCAGAGC ATTCCGCAGC AAAGTAAGGA AGCAAATCAA 
CTTTGGTCAT CAATGATGCC AAATCACATG CCCTTTGTAT ATAGCATTCC TCCTAAGTGT 
GTGACTCCAT CTACCAATGT TGATGTGTTT CCTCATTCTC CTGGCACTGT GCTCAAGGAA 
AATATGAATG GGGATCGTGT TCTTAAGTTT CCAAACAAGA ATGCTGCCAA CCTTGGAAAA 
CAAAACAGGA ATCTTGGCTC AGAAACGCTC CTCAGGGCAC ATGCAGAATA CCCTTTTGCT 
GGAAAACATA ATGGGATTGA GCTTAATCAT AAGCCAATGG GGTCCTTGGA ACTGTATTCT 
AATGAAACAA TACCAGCTAT GCACTTACTC AGCCTCATGG ACGCAGGAGT GCAGTCCAGT 
GCACCGATCA ATATGGATGT AAACCCAAAG TTCCTCAAGA GACCTGCCAT TATTCACAAT 
GCTGAACCAA AGGAGTTTTC TAGGCTGGAC ACTGGGGCAT ATAAAGTCAT TTCTTCTGTG 
AAGCACCCAC CACGTAATCA TAATGGTAAA AATCAACTTG CAGAGAGTTC CCGTGACCTT 
ATTCCTATCA TGCAAACAAC AGCTGGTGCA TCTTCTTTGT CAATTCGACA TGATAAACGT 
ATTAGGAAGC CTGTTGATCT TCCCAGTCCA GTTATTCAAT ATAAAGAGAG GAGAAAAGGC 
TCTGATTCAC GCACACAGAA TAAAGCAAAT AGGTCACAGA CATCAGCAAA TGGTCGTTTT 
GGCACCAACT GTGGATCCAT TCCAGCTCAT AGCATGCGGA TAATGTCTTT TGGTGCTCCA 
GATCCTTCAG TGTTTTCATT GCCATTCCGT GCATTGGAAA ACCCAAACAA GGACAAGTTG 
AAGTCCCTTG ATAACAATAG AATTGTTCAC CCCCACAAAA GCAGCTCTGA GACTGAAGTC 
TGCAGTGTGA ACAGAAACCC TGCTGATTTC ACCATACCGG AAGCTGGAAA TATGTATATG 
ATTGCAGGTG AAGCCCTTCG ATTTGAAAAG GATGTTCCTT TTGCAAATGG ATCTCACTCG 
CTTAAATTGG ATGGGCGCAA ACGACAGAGG AAGCTTCCTG CTATGAAAGA CCATGGACGA 
CCTCCAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024446342.1
CDS	326..4060
Translation	MCWPFVSDGDSNNYEEQACLLPPLHVPKFRFWRCQNCVWEVGAKGTANCYGTALKSCGAELKSTNVCSHGPNFDDAARLPSDVQEATNQEIPEGRQADVFASLNSTSKSHHSLSIDKNEKKTKDMNGSIIGKHMGSEDNLKQENHRLACVVTEVVSSPIQKADHSDEIVAFKSIGIDLCESGCGHHEVADAEFSKNLNCMVNNATGLCEAGKETSIDDQHKELIACGISGEVGNIDDGALSTANKDPVSRPSLELDEYDDPSSESAEIMVGHNTQDVLHENSSGLHRRKTRKVRLLTELLCENGDRDTDNRTRFSLPHAFPDASAGVDKISVLQGEVAIQGKVRRGLGHNSKRKLPQDEDSRSLEMRSPNKVCKEVGILKRDGESAEPIVASESEEDASGRMGLQTGMKIQWAKNKVDRSPIVSKKKNKKVPSFDECLSPDPSQENLPNEIGEKNGDNSRPIAVDGVLAKSVHNAFIGREMDLFPLPDPRMEKNVSEYKKKGKMPQFDDYQVSPTPWNHDILREGPVIRKDVGTINTGPVVFPFHSAQETSLEKGLDLSLNSYKTAQSYDGKHIPLVENRRSCLFTWQEGTSQIQAKRKASKIEHAGNISFTSKIAQDAPFEKGLHCDPNTKRPSFEMPFRREKQKYTSQVEIGGCSLMQKKDFCHNKGNEGTIGILEHSAFPRKDVNQRADKVCEQGALDDIPMEIVELMAKNQYERCLPDGEYEKCQLETTSSSRRSQTINFSQLLGLGGLSLFHQETTRKQNPPARRNDIIKIGEMVGQTKQKEVDFFSQADRNPFSMRQPEKIRSPVGFGAFLQLQEKPSGRFQHPASSYNIQNTPQICKQRGEVVGNRSCHTRFQTPGACNTCQSIPQQSKEANQLWSSMMPNHMPFVYSIPPKCVTPSTNVDVFPHSPGTVLKENMNGDRVLKFPNKNAANLGKQNRNLGSETLLRAHAEYPFAGKHNGIELNHKPMGSLELYSNETIPAMHLLSLMDAGVQSSAPINMDVNPKFLKRPAIIHNAEPKEFSRLDTGAYKVISSVKHPPRNHNGKNQLAESSRDLIPIMQTTAGASSLSIRHDKRIRKPVDLPSPVIQYKERRKGSDSRTQNKANRSQTSANGRFGTNCGSIPAHSMRIMSFGAPDPSVFSLPFRALENPNKDKLKSLDNNRIVHPHKSSSETEVCSVNRNPADFTIPEAGNMYMIAGEALRFEKDVPFANGSHSLKLDGRKRQRKLPAMKDHGRPPMS

Target ORF information:

RefSeq Version	XM_024590574.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein EMBRYONIC FLOWER 1 (LOC7458970), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024590574.1

ORF Insert Sequence:

ATGTGTTGGC CGTTTGTGTC AGATGGTGAC AGTAATAACT ATGAGGAGCA AGCATGTCTG 
CTTCCTCCTT TACATGTTCC GAAATTCCGA TTTTGGCGTT GTCAGAATTG TGTGTGGGAA 
GTTGGTGCCA AAGGCACTGC AAACTGTTAT GGAACTGCTC TAAAATCATG CGGAGCTGAA 
CTCAAATCCA CCAACGTTTG TTCCCATGGA CCAAACTTTG ATGATGCTGC AAGACTACCA 
TCTGATGTTC AGGAAGCCAC AAATCAAGAA ATTCCTGAGG GAAGACAAGC TGATGTTTTT 
GCTAGTTTGA ACAGTACCAG TAAATCTCAT CATTCACTGT CCATTGATAA AAATGAAAAG 
AAGACTAAGG ATATGAATGG TTCCATCATA GGGAAACATA TGGGGTCAGA AGATAATTTG 
AAGCAGGAAA ATCATAGACT GGCATGCGTA GTAACTGAAG TTGTTTCAAG CCCAATTCAG 
AAGGCAGATC ATTCAGATGA AATAGTAGCT TTCAAGTCAA TAGGTATTGA CTTATGTGAG 
TCAGGCTGTG GACATCATGA AGTTGCTGAT GCTGAGTTTT CTAAAAACCT TAATTGCATG 
GTAAATAATG CTACTGGACT TTGTGAAGCA GGAAAAGAAA CTTCCATAGA TGATCAACAC 
AAAGAGCTAA TAGCTTGTGG TATATCTGGA GAAGTTGGTA ACATTGATGA TGGGGCACTC 
AGTACTGCCA ACAAGGATCC TGTGAGCCGC CCTTCCCTGG AATTAGATGA GTATGATGAT 
CCTTCATCTG AAAGTGCTGA GATCATGGTT GGTCATAATA CTCAGGATGT TCTTCATGAA 
AATTCAAGTG GTTTGCATCG TAGAAAAACT CGAAAGGTAC GCTTGTTGAC GGAATTGTTG 
TGTGAAAATG GGGATAGAGA CACTGATAAC AGAACTCGGT TTTCTCTGCC CCATGCCTTT 
CCTGATGCAT CTGCTGGAGT TGATAAGATT TCTGTTCTCC AAGGGGAGGT GGCTATCCAA 
GGCAAAGTTA GAAGGGGCTT GGGTCATAAT AGTAAAAGAA AGTTGCCTCA GGATGAAGAT 
TCAAGATCTC TGGAGATGAG GTCTCCCAAT AAAGTTTGCA AAGAAGTTGG GATCTTAAAG 
AGAGATGGGG AATCTGCTGA ACCCATTGTG GCTTCTGAAT CAGAAGAAGA TGCATCTGGT 
AGAATGGGTT TACAAACTGG CATGAAGATT CAGTGGGCCA AAAACAAAGT TGATAGAAGT 
CCCATTGTAA GTAAGAAGAA AAACAAAAAG GTACCAAGTT TTGATGAATG TTTGTCTCCA 
GACCCATCCC AAGAAAATTT GCCAAATGAA ATTGGGGAGA AAAATGGAGA TAATTCTAGG 
CCCATTGCTG TTGATGGTGT ACTGGCCAAG TCAGTACACA ATGCATTTAT AGGCAGAGAG 
ATGGACCTTT TCCCTTTGCC TGATCCAAGA ATGGAGAAAA ATGTTAGTGA GTATAAGAAG 
AAAGGAAAGA TGCCTCAGTT TGATGATTAT CAGGTTTCCC CTACTCCTTG GAATCATGAC 
ATTCTCAGAG AAGGTCCAGT GATTAGAAAA GATGTGGGGA CAATAAATAC TGGACCTGTA 
GTTTTTCCAT TTCATTCAGC ACAGGAAACA TCCCTTGAAA AGGGGCTGGA TCTTTCTCTT 
AACAGCTACA AGACTGCACA AAGCTATGAT GGGAAACATA TTCCACTGGT AGAAAATAGG 
CGAAGTTGTT TATTTACTTG GCAAGAAGGA ACTTCCCAAA TTCAGGCCAA GAGGAAAGCT 
TCAAAAATTG AGCACGCGGG GAATATCAGT TTCACTTCTA AAATTGCCCA AGATGCACCT 
TTTGAGAAAG GACTACATTG TGATCCCAAT ACCAAGAGAC CATCTTTTGA AATGCCCTTC 
CGCAGAGAGA AGCAGAAATA CACCTCCCAG GTTGAAATTG GGGGTTGCTC TCTCATGCAG 
AAAAAGGACT TTTGTCACAA TAAAGGCAAT GAGGGAACCA TTGGAATACT GGAGCATTCA 
GCTTTTCCAA GGAAAGATGT CAACCAAAGA GCAGACAAGG TGTGTGAACA GGGAGCTTTA 
GATGACATTC CAATGGAAAT TGTAGAACTC ATGGCAAAGA ATCAGTATGA GAGGTGTCTT 
CCTGATGGTG AATACGAGAA ATGCCAGTTA GAAACAACTA GTAGTTCAAG GAGGAGTCAG 
ACAATAAATT TCAGTCAATT ATTGGGGCTT GGAGGATTAA GCTTATTTCA TCAGGAAACT 
ACTCGGAAAC AGAACCCTCC AGCTAGAAGA AATGACATAA TAAAGATTGG TGAAATGGTA 
GGACAAACTA AACAAAAGGA AGTGGATTTC TTTTCTCAAG CTGATCGAAA CCCATTCAGC 
ATGAGGCAAC CGGAAAAAAT TCGTTCCCCT GTAGGGTTTG GGGCATTTCT ACAACTTCAA 
GAGAAGCCAT CAGGCAGATT CCAACATCCT GCTTCCAGTT ATAACATACA AAACACTCCT 
CAAATTTGCA AACAGAGAGG GGAAGTGGTG GGGAATAGGT CTTGTCATAC CAGGTTCCAG 
ACCCCAGGAG CATGTAACAC ATGCCAGAGC ATTCCGCAGC AAAGTAAGGA AGCAAATCAA 
CTTTGGTCAT CAATGATGCC AAATCACATG CCCTTTGTAT ATAGCATTCC TCCTAAGTGT 
GTGACTCCAT CTACCAATGT TGATGTGTTT CCTCATTCTC CTGGCACTGT GCTCAAGGAA 
AATATGAATG GGGATCGTGT TCTTAAGTTT CCAAACAAGA ATGCTGCCAA CCTTGGAAAA 
CAAAACAGGA ATCTTGGCTC AGAAACGCTC CTCAGGGCAC ATGCAGAATA CCCTTTTGCT 
GGAAAACATA ATGGGATTGA GCTTAATCAT AAGCCAATGG GGTCCTTGGA ACTGTATTCT 
AATGAAACAA TACCAGCTAT GCACTTACTC AGCCTCATGG ACGCAGGAGT GCAGTCCAGT 
GCACCGATCA ATATGGATGT AAACCCAAAG TTCCTCAAGA GACCTGCCAT TATTCACAAT 
GCTGAACCAA AGGAGTTTTC TAGGCTGGAC ACTGGGGCAT ATAAAGTCAT TTCTTCTGTG 
AAGCACCCAC CACGTAATCA TAATGGTAAA AATCAACTTG CAGAGAGTTC CCGTGACCTT 
ATTCCTATCA TGCAAACAAC AGCTGGTGCA TCTTCTTTGT CAATTCGACA TGATAAACGT 
ATTAGGAAGC CTGTTGATCT TCCCAGTCCA GTTATTCAAT ATAAAGAGAG GAGAAAAGGC 
TCTGATTCAC GCACACAGAA TAAAGCAAAT AGGTCACAGA CATCAGCAAA TGGTCGTTTT 
GGCACCAACT GTGGATCCAT TCCAGCTCAT AGCATGCGGA TAATGTCTTT TGGTGCTCCA 
GATCCTTCAG TGTTTTCATT GCCATTCCGT GCATTGGAAA ACCCAAACAA GGACAAGTTG 
AAGTCCCTTG ATAACAATAG AATTGTTCAC CCCCACAAAA GCAGCTCTGA GACTGAAGTC 
TGCAGTGTGA ACAGAAACCC TGCTGATTTC ACCATACCGG AAGCTGGAAA TATGTATATG 
ATTGCAGGTG AAGCCCTTCG ATTTGAAAAG GATGTTCCTT TTGCAAATGG ATCTCACTCG 
CTTAAATTGG ATGGGCGCAA ACGACAGAGG AAGCTTCCTG CTATGAAAGA CCATGGACGA 
CCTCCAATGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7458970 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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