POPTR_0006s13410g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0006s13410g
Entrez Gene ID	18100234
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006381434.2	XP_006381496.1	E3 ubiquitin-protein ligase UPL4 isoform X1
XM_024604760.1	XP_024460528.1	E3 ubiquitin-protein ligase UPL4 isoform X1
XM_024604761.1	XP_024460529.1	E3 ubiquitin-protein ligase UPL4 isoform X2
XM_006381434.1	XP_006381496.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0006s13410g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0006s13410g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa16362	XM_006381434.2 Latest version!	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPa16362	XM_024604760.1 Latest version!	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPa432786	XM_024604761.1 Latest version!	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OPa16362	XM_006381434.1	Populus trichocarpa hypothetical protein (POPTR_0006s13410g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0006s13410g ( XM_006381434.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_006381496.1 Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa16362
Clone ID Related Accession (Same CDS sequence)	XM_006381434.2 , XM_024604760.1 , XM_006381434.1
Accession Version	XM_006381434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	E3 ubiquitin-protein ligase UPL4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_006381434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006381496.1
CDS	281..5005
Translation	MGNRGQKRAEMVNELPSDKRACSSLEFRPSSSNSSIQTQINSENHNAETHDADMDTSSSGSASSHSDEEEPERDSAYGSCDSEGPRHSSLREYQRQRSSGDHSRLRDCLSNLTEGTEPSGQLAALTELCEVLSFCTEDSLSSTMADSLSPVLVMLSRLESNPDIMLLAIRALTYLCDVFPRASVFLVKHNAIPAICQRLMAIEYLDVAEQCLQALEKISRDQPLPCLQAGAIMAVLSFVDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPRLCNLLQYEDRQLVENVAICLIKIAERVSQLSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLCSGSVVAFKTLYELNISSILKDILTTYDLSHGMSSPHVVDGQGNQVHEVLKLLNVLLPIIARDQDVQQHVLDKETFLANHPKLLQKFGLDIIPSLIQVVNSGANLYVCYGCLCVINKLVYLSKSDMLLELLKNTNIPSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDALLVSEKCSQLMFPVCSGIQLPIDASLKSSSKVVMRCLCYAFDTGQSLSTTETGTCKLEKDSVQNLAKHIRTSCFASELCNSEKGLTDILQKLRALSAELSDLMNMPGNIGSCTQDEEKCYCVLRQIIEKLDGREPVSTFEFIESGIVKILVNYLSSGKYLREKVEPQGTLDDCDVIEKRFEVFARLLLSSPDLSVEFPLSVLIQKLQGALSSLENFPVILSHASKQRSSFAIIPTGHCTSYPCLRVRFVRGKGETCLCDYSEDVVTVDPLSSVDAIEGYLSPKVRIKGTEQIESAAQAIEGALSAENAQFKSPSTANSSQGESSGLMEPDSIATDLPVMQEDEANLSQSPPEPDVNLLQRNPDETTSSNNTHNVSVEKIVQSPSCADVTTKGHCLMSCSNGDALPKLVFYLEGQRLDQTLTLYQAILQQKVKADREINSTAKLWTQVHTLTYGMVVDPKDDSPPDHSSTAQNSSMLDQVGAYMQHPAFFSSLFNGELTSDLDKYSPTNDVLFLLKSLEGLNRFIFHLMSRERIHAFAEGLIDNLGYLKVAVRPVSQNEFVSCKLTEKLEQQMRDSLAVSIGGMPVWCNQLMDSCSFLFSFEARCKYFRLSAFGRQQVQPQPSSHNNSGVSRDGPPSAGSLSRKKFLVLRDRVLESAAQMMDSYAHVKAPIEVEYNEEVGTGLGPTLEFYTLVSREFQKSGLGMWRQDHISFTTSETLQAEYSGIVNSSFGLFPRPWPSSVDASDAAQFSEVIKKFFLLGQIVAKALQDGRVLDLPFSKAFYKLILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGSAFGENSSSALDACFWNTKIEDLYLDFTLPGYPDYVLSFDEDHKIVNMVNLDAYVSRIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGEREFWAFNELLDHIKFDHGYTASSPPVVNLLEIIKEFEYEQLRSFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNCADADLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS

Target ORF information:

RefSeq Version	XM_006381434.2
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006381434.2

ORF Insert Sequence:

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s13410g ( XM_024604760.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024460528.1 Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa16362
Clone ID Related Accession (Same CDS sequence)	XM_006381434.2 , XM_024604760.1 , XM_006381434.1
Accession Version	XM_024604760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	E3 ubiquitin-protein ligase UPL4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024460528.1
CDS	281..5005
Translation	MGNRGQKRAEMVNELPSDKRACSSLEFRPSSSNSSIQTQINSENHNAETHDADMDTSSSGSASSHSDEEEPERDSAYGSCDSEGPRHSSLREYQRQRSSGDHSRLRDCLSNLTEGTEPSGQLAALTELCEVLSFCTEDSLSSTMADSLSPVLVMLSRLESNPDIMLLAIRALTYLCDVFPRASVFLVKHNAIPAICQRLMAIEYLDVAEQCLQALEKISRDQPLPCLQAGAIMAVLSFVDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPRLCNLLQYEDRQLVENVAICLIKIAERVSQLSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLCSGSVVAFKTLYELNISSILKDILTTYDLSHGMSSPHVVDGQGNQVHEVLKLLNVLLPIIARDQDVQQHVLDKETFLANHPKLLQKFGLDIIPSLIQVVNSGANLYVCYGCLCVINKLVYLSKSDMLLELLKNTNIPSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDALLVSEKCSQLMFPVCSGIQLPIDASLKSSSKVVMRCLCYAFDTGQSLSTTETGTCKLEKDSVQNLAKHIRTSCFASELCNSEKGLTDILQKLRALSAELSDLMNMPGNIGSCTQDEEKCYCVLRQIIEKLDGREPVSTFEFIESGIVKILVNYLSSGKYLREKVEPQGTLDDCDVIEKRFEVFARLLLSSPDLSVEFPLSVLIQKLQGALSSLENFPVILSHASKQRSSFAIIPTGHCTSYPCLRVRFVRGKGETCLCDYSEDVVTVDPLSSVDAIEGYLSPKVRIKGTEQIESAAQAIEGALSAENAQFKSPSTANSSQGESSGLMEPDSIATDLPVMQEDEANLSQSPPEPDVNLLQRNPDETTSSNNTHNVSVEKIVQSPSCADVTTKGHCLMSCSNGDALPKLVFYLEGQRLDQTLTLYQAILQQKVKADREINSTAKLWTQVHTLTYGMVVDPKDDSPPDHSSTAQNSSMLDQVGAYMQHPAFFSSLFNGELTSDLDKYSPTNDVLFLLKSLEGLNRFIFHLMSRERIHAFAEGLIDNLGYLKVAVRPVSQNEFVSCKLTEKLEQQMRDSLAVSIGGMPVWCNQLMDSCSFLFSFEARCKYFRLSAFGRQQVQPQPSSHNNSGVSRDGPPSAGSLSRKKFLVLRDRVLESAAQMMDSYAHVKAPIEVEYNEEVGTGLGPTLEFYTLVSREFQKSGLGMWRQDHISFTTSETLQAEYSGIVNSSFGLFPRPWPSSVDASDAAQFSEVIKKFFLLGQIVAKALQDGRVLDLPFSKAFYKLILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGSAFGENSSSALDACFWNTKIEDLYLDFTLPGYPDYVLSFDEDHKIVNMVNLDAYVSRIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGEREFWAFNELLDHIKFDHGYTASSPPVVNLLEIIKEFEYEQLRSFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNCADADLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS

Target ORF information:

RefSeq Version	XM_024604760.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024604760.1

ORF Insert Sequence:

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s13410g ( XM_006381434.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_006381496.1 Populus trichocarpa hypothetical protein (POPTR_0006s13410g) mRNA, complete cds.

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CloneID	OPa16362
Clone ID Related Accession (Same CDS sequence)	XM_006381434.2 , XM_024604760.1 , XM_006381434.1
Accession Version	XM_006381434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008472). COMPLETENESS: incomplete on the 5' end.

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006381496.1
CDS	1..4725
Translation	MGNRGQKRAEMVNELPSDKRACSSLEFRPSSSNSSIQTQINSENHNAETHDADMDTSSSGSASSHSDEEEPERDSAYGSCDSEGPRHSSLREYQRQRSSGDHSRLRDCLSNLTEGTEPSGQLAALTELCEVLSFCTEDSLSSTMADSLSPVLVMLSRLESNPDIMLLAIRALTYLCDVFPRASVFLVKHNAIPAICQRLMAIEYLDVAEQCLQALEKISRDQPLPCLQAGAIMAVLSFVDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPRLCNLLQYEDRQLVENVAICLIKIAERVSQLSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLCSGSVVAFKTLYELNISSILKDILTTYDLSHGMSSPHVVDGQGNQVHEVLKLLNVLLPIIARDQDVQQHVLDKETFLANHPKLLQKFGLDIIPSLIQVVNSGANLYVCYGCLCVINKLVYLSKSDMLLELLKNTNIPSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDALLVSEKCSQLMFPVCSGIQLPIDASLKSSSKVVMRCLCYAFDTGQSLSTTETGTCKLEKDSVQNLAKHIRTSCFASELCNSEKGLTDILQKLRALSAELSDLMNMPGNIGSCTQDEEKCYCVLRQIIEKLDGREPVSTFEFIESGIVKILVNYLSSGKYLREKVEPQGTLDDCDVIEKRFEVFARLLLSSPDLSVEFPLSVLIQKLQGALSSLENFPVILSHASKQRSSFAIIPTGHCTSYPCLRVRFVRGKGETCLCDYSEDVVTVDPLSSVDAIEGYLSPKVRIKGTEQIESAAQAIEGALSAENAQFKSPSTANSSQGESSGLMEPDSIATDLPVMQEDEANLSQSPPEPDVNLLQRNPDETTSSNNTHNVSVEKIVQSPSCADVTTKGHCLMSCSNGDALPKLVFYLEGQRLDQTLTLYQAILQQKVKADREINSTAKLWTQVHTLTYGMVVDPKDDSPPDHSSTAQNSSMLDQVGAYMQHPAFFSSLFNGELTSDLDKYSPTNDVLFLLKSLEGLNRFIFHLMSRERIHAFAEGLIDNLGYLKVAVRPVSQNEFVSCKLTEKLEQQMRDSLAVSIGGMPVWCNQLMDSCSFLFSFEARCKYFRLSAFGRQQVQPQPSSHNNSGVSRDGPPSAGSLSRKKFLVLRDRVLESAAQMMDSYAHVKAPIEVEYNEEVGTGLGPTLEFYTLVSREFQKSGLGMWRQDHISFTTSETLQAEYSGIVNSSFGLFPRPWPSSVDASDAAQFSEVIKKFFLLGQIVAKALQDGRVLDLPFSKAFYKLILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGSAFGENSSSALDACFWNTKIEDLYLDFTLPGYPDYVLSFDEDHKIVNMVNLDAYVSRIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGEREFWAFNELLDHIKFDHGYTASSPPVVNLLEIIKEFEYEQLRSFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNCADADLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS

Target ORF information:

RefSeq Version	XM_006381434.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0006s13410g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006381434.1

ORF Insert Sequence:

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GTAATGCAGG AAGATGAAGC TAACCTATCT 
CAGTCTCCAC CAGAACCAGA TGTAAATTTG TTGCAGAGAA ATCCTGATGA AACAACATCC 
TCAAATAATA CTCATAATGT CTCAGTGGAG AAAATAGTGC AGTCTCCTTC ATGTGCTGAT 
GTCACTACAA AAGGTCATTG TCTTATGTCC TGTAGCAATG GAGATGCTCT GCCGAAACTG 
GTATTTTACC TAGAAGGGCA GCGGCTGGAC CAAACTTTGA CTCTTTACCA GGCAATTCTC 
CAGCAAAAAG TTAAAGCAGA CCGTGAAATT AACAGCACTG CAAAGCTGTG GACTCAAGTA 
CACACCTTGA CTTACGGAAT GGTTGTGGAC CCTAAAGATG ATAGTCCTCC AGATCATTCT 
AGTACAGCTC AAAATTCCTC TATGTTAGAT CAAGTTGGGG CTTATATGCA GCATCCTGCA 
TTTTTCTCAA GCTTGTTTAA TGGCGAACTC ACTTCTGACC TGGATAAGTA TAGTCCTACT 
AATGATGTAT TGTTTCTGCT TAAAAGTTTA GAAGGTTTAA ACAGGTTTAT ATTTCATCTG 
ATGTCTCGTG AAAGAATCCA TGCATTTGCT GAAGGGCTGA TTGATAATTT GGGTTATTTA 
AAGGTAGCAG TTCGCCCAGT ATCACAAAAT GAATTTGTGA GCTGTAAGTT GACCGAGAAA 
TTGGAGCAAC AGATGCGGGA TTCTCTGGCT GTGTCCATAG GTGGCATGCC TGTGTGGTGC 
AATCAGCTCA TGGATTCATG CTCCTTTTTG TTTAGCTTTG AGGCTAGATG TAAATATTTC 
CGCTTGTCAG CATTCGGCCG TCAGCAAGTT CAACCACAGC CATCATCACA TAATAATTCA 
GGTGTCTCAA GGGACGGACC ACCAAGTGCT GGCAGTTTGT CCCGTAAGAA ATTCCTAGTT 
TTGCGTGACC GAGTTCTGGA GTCTGCTGCC CAGATGATGG ATAGTTATGC TCATGTTAAA 
GCACCTATTG AAGTGGAATA TAATGAAGAA GTTGGTACTG GTCTCGGTCC AACCTTGGAA 
TTTTATACTC TGGTTAGTCG TGAGTTTCAG AAGTCTGGCC TTGGTATGTG GAGGCAGGAT 
CATATTTCAT TTACCACCAG CGAGACCCTG CAGGCTGAAT ACTCTGGAAT TGTGAACTCT 
TCATTTGGAC TTTTTCCTCG CCCATGGCCG TCTTCAGTGG ATGCATCTGA TGCGGCACAG 
TTTTCTGAAG TTATTAAAAA GTTTTTCCTT CTGGGACAAA TTGTAGCCAA GGCTCTTCAA 
GATGGGAGGG TGCTGGATCT ACCATTCTCT AAAGCCTTCT ATAAGCTTAT TCTTCAGCAG 
GAACTTAATT TGTATGACAT CCAGTCCTTT GATCCCGAGC TTGGTAGGAC ATTGCTAGAG 
TTCCAGGCTC TTGTCAACAG GAAAAAAAAT ATGGGATCAG CCTTTGGAGA AAACTCATCT 
TCTGCTCTAG ATGCATGCTT TTGGAACACC AAAATCGAGG ATCTTTATCT TGACTTTACT 
CTTCCTGGGT ATCCTGATTA CGTTCTTAGT TTTGATGAAG ATCACAAAAT TGTGAATATG 
GTTAACTTGG ATGCTTATGT TTCTCGCATT GTGGATGCTA CTATACACAC AGGAATTTCC 
AGACAAGTTG AAGCATTCAA GTCTGGATTT AACCAGGTTT TTCCTATTAA ACATCTTATG 
ATTTTCACCG AGGAGGAACT TGAGCGTTTG CTATGTGGAG AACGTGAATT TTGGGCTTTC 
AATGAGCTTT TGGACCATAT CAAGTTTGAT CATGGATACA CTGCTAGCAG CCCTCCTGTT 
GTTAATTTGC TGGAAATCAT AAAGGAATTC GAATACGAAC AACTACGATC ATTTCTACAG 
TTTGTAACCG GGGCACCTCG GCTACCTACA GGAGGCTTGG CATCTCTGAA TCCAAAATTG 
ACCATTGTCC GGAAGCATTG TAGCAACTGT GCGGATGCGG ACCTTCCCAG TGTGATGACT 
TGTGCCAATT ATCTGAAGTT GCCACCTTAC TCGTCAAAAG ACAAGATGAA AGAGAAACTT 
CTGTATGCCA TAACAGAGGG TCAAGGATCA TTCCACCTCT CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0006s13410g ( XM_024604761.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024460529.1 Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X3, mRNA.

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CloneID	OPa432786
Clone ID Related Accession (Same CDS sequence)	XM_024604761.1
Accession Version	XM_024604761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	E3 ubiquitin-protein ligase UPL4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037290.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GAAGATGAAG CTAACCTATC TCAGTCTCCA 
CCAGAACCAG ATGTAAATTT GTTGCAGAGA AATCCTGATG AAACAACATC CTCAAATAAT 
ACTCATAATG TCTCAGTGGA GAAAATAGTG CAGTCTCCTT CATGTGCTGA TGTCACTACA 
AAAGGTCATT GTCTTATGTC CTGTAGCAAT GGAGATGCTC TGCCGAAACT GGTATTTTAC 
CTAGAAGGGC AGCGGCTGGA CCAAACTTTG ACTCTTTACC AGGCAATTCT CCAGCAAAAA 
GTTAAAGCAG ACCGTGAAAT TAACAGCACT GCAAAGCTGT GGACTCAAGT ACACACCTTG 
ACTTACGGAA TGGTTGTGGA CCCTAAAGAT GATAGTCCTC CAGATCATTC TAGTACAGCT 
CAAAATTCCT CTATGTTAGA TCAAGTTGGG GCTTATATGC AGCATCCTGC ATTTTTCTCA 
AGCTTGTTTA ATGGCGAACT CACTTCTGAC CTGGATAAGT ATAGTCCTAC TAATGATGTA 
TTGTTTCTGC TTAAAAGTTT AGAAGGTTTA AACAGGTTTA TATTTCATCT GATGTCTCGT 
GAAAGAATCC ATGCATTTGC TGAAGGGCTG ATTGATAATT TGGGTTATTT AAAGGTAGCA 
GTTCGCCCAG TATCACAAAA TGAATTTGTG AGCTGTAAGT TGACCGAGAA ATTGGAGCAA 
CAGATGCGGG ATTCTCTGGC TGTGTCCATA GGTGGCATGC CTGTGTGGTG CAATCAGCTC 
ATGGATTCAT GCTCCTTTTT GTTTAGCTTT GAGGCTAGAT GTAAATATTT CCGCTTGTCA 
GCATTCGGCC GTCAGCAAGT TCAACCACAG CCATCATCAC ATAATAATTC AGGTGTCTCA 
AGGGACGGAC CACCAAGTGC TGGCAGTTTG TCCCGTAAGA AATTCCTAGT TTTGCGTGAC 
CGAGTTCTGG AGTCTGCTGC CCAGATGATG GATAGTTATG CTCATGTTAA AGCACCTATT 
GAAGTGGAAT ATAATGAAGA AGTTGGTACT GGTCTCGGTC CAACCTTGGA ATTTTATACT 
CTGGTTAGTC GTGAGTTTCA GAAGTCTGGC CTTGGTATGT GGAGGCAGGA TCATATTTCA 
TTTACCACCA GCGAGACCCT GCAGGCTGAA TACTCTGGAA TTGTGAACTC TTCATTTGGA 
CTTTTTCCTC GCCCATGGCC GTCTTCAGTG GATGCATCTG ATGCGGCACA GTTTTCTGAA 
GTTATTAAAA AGTTTTTCCT TCTGGGACAA ATTGTAGCCA AGGCTCTTCA AGATGGGAGG 
GTGCTGGATC TACCATTCTC TAAAGCCTTC TATAAGCTTA TTCTTCAGCA GGAACTTAAT 
TTGTATGACA TCCAGTCCTT TGATCCCGAG CTTGGTAGGA CATTGCTAGA GTTCCAGGCT 
CTTGTCAACA GGAAAAAAAA TATGGGATCA GCCTTTGGAG AAAACTCATC TTCTGCTCTA 
GATGCATGCT TTTGGAACAC CAAAATCGAG GATCTTTATC TTGACTTTAC TCTTCCTGGG 
TATCCTGATT ACGTTCTTAG TTTTGATGAA GATCACAAAA TTGTGAATAT GGTTAACTTG 
GATGCTTATG TTTCTCGCAT TGTGGATGCT ACTATACACA CAGGAATTTC CAGACAAGTT 
GAAGCATTCA AGTCTGGATT TAACCAGGTT TTTCCTATTA AACATCTTAT GATTTTCACC 
GAGGAGGAAC TTGAGCGTTT GCTATGTGGA GAACGTGAAT TTTGGGCTTT CAATGAGCTT 
TTGGACCATA TCAAGTTTGA TCATGGATAC ACTGCTAGCA GCCCTCCTGT TGTTAATTTG 
CTGGAAATCA TAAAGGAATT CGAATACGAA CAACTACGAT CATTTCTACA GTTTGTAACC 
GGGGCACCTC GGCTACCTAC AGGAGGCTTG GCATCTCTGA ATCCAAAATT GACCATTGTC 
CGGAAGCATT GTAGCAACTG TGCGGATGCG GACCTTCCCA GTGTGATGAC TTGTGCCAAT 
TATCTGAAGT TGCCACCTTA CTCGTCAAAA GACAAGATGA AAGAGAAACT TCTGTATGCC 
ATAACAGAGG GTCAAGGATC ATTCCACCTC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024460529.1
CDS	281..4996
Translation	MGNRGQKRAEMVNELPSDKRACSSLEFRPSSSNSSIQTQINSENHNAETHDADMDTSSSGSASSHSDEEEPERDSAYGSCDSEGPRHSSLREYQRQRSSGDHSRLRDCLSNLTEGTEPSGQLAALTELCEVLSFCTEDSLSSTMADSLSPVLVMLSRLESNPDIMLLAIRALTYLCDVFPRASVFLVKHNAIPAICQRLMAIEYLDVAEQCLQALEKISRDQPLPCLQAGAIMAVLSFVDFFSTSVQRVALSTVVNICKKLPSENFSPFMEAVPRLCNLLQYEDRQLVENVAICLIKIAERVSQLSEMLDELCKHGLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLCSGSVVAFKTLYELNISSILKDILTTYDLSHGMSSPHVVDGQGNQVHEVLKLLNVLLPIIARDQDVQQHVLDKETFLANHPKLLQKFGLDIIPSLIQVVNSGANLYVCYGCLCVINKLVYLSKSDMLLELLKNTNIPSFLAGVLTRKDHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDALLVSEKCSQLMFPVCSGIQLPIDASLKSSSKVVMRCLCYAFDTGQSLSTTETGTCKLEKDSVQNLAKHIRTSCFASELCNSEKGLTDILQKLRALSAELSDLMNMPGNIGSCTQDEEKCYCVLRQIIEKLDGREPVSTFEFIESGIVKILVNYLSSGKYLREKVEPQGTLDDCDVIEKRFEVFARLLLSSPDLSVEFPLSVLIQKLQGALSSLENFPVILSHASKQRSSFAIIPTGHCTSYPCLRVRFVRGKGETCLCDYSEDVVTVDPLSSVDAIEGYLSPKVRIKGTEQIESAAQAIEGALSAENAQFKSPSTANSSQGESSGLMEPDSIATDLPEDEANLSQSPPEPDVNLLQRNPDETTSSNNTHNVSVEKIVQSPSCADVTTKGHCLMSCSNGDALPKLVFYLEGQRLDQTLTLYQAILQQKVKADREINSTAKLWTQVHTLTYGMVVDPKDDSPPDHSSTAQNSSMLDQVGAYMQHPAFFSSLFNGELTSDLDKYSPTNDVLFLLKSLEGLNRFIFHLMSRERIHAFAEGLIDNLGYLKVAVRPVSQNEFVSCKLTEKLEQQMRDSLAVSIGGMPVWCNQLMDSCSFLFSFEARCKYFRLSAFGRQQVQPQPSSHNNSGVSRDGPPSAGSLSRKKFLVLRDRVLESAAQMMDSYAHVKAPIEVEYNEEVGTGLGPTLEFYTLVSREFQKSGLGMWRQDHISFTTSETLQAEYSGIVNSSFGLFPRPWPSSVDASDAAQFSEVIKKFFLLGQIVAKALQDGRVLDLPFSKAFYKLILQQELNLYDIQSFDPELGRTLLEFQALVNRKKNMGSAFGENSSSALDACFWNTKIEDLYLDFTLPGYPDYVLSFDEDHKIVNMVNLDAYVSRIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGEREFWAFNELLDHIKFDHGYTASSPPVVNLLEIIKEFEYEQLRSFLQFVTGAPRLPTGGLASLNPKLTIVRKHCSNCADADLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS

Target ORF information:

RefSeq Version	XM_024604761.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa E3 ubiquitin-protein ligase UPL4 (LOC18100234), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024604761.1

ORF Insert Sequence:

ATGGGAAATC GTGGACAAAA ACGAGCTGAG ATGGTTAATG AATTGCCCTC TGATAAGAGA 
GCTTGTAGTT CATTGGAGTT TAGACCAAGT TCATCGAACT CATCAATTCA AACTCAAATA 
AATTCTGAGA ATCATAATGC TGAAACCCAT GATGCTGACA TGGATACTTC ATCATCTGGG 
TCGGCATCAA GCCATTCAGA CGAAGAAGAA CCTGAGAGGG ATTCAGCATA TGGCTCTTGT 
GATTCTGAGG GTCCAAGACA TAGCAGTTTG CGTGAGTATC AGAGGCAGAG ATCCTCTGGT 
GATCATAGCA GATTGAGGGA TTGTCTATCT AATTTAACTG AAGGAACTGA GCCTTCTGGG 
CAGTTAGCTG CTCTTACAGA GTTATGTGAA GTGCTTTCAT TTTGTACAGA GGATTCACTT 
TCAAGTACGA TGGCAGACTC TTTGTCACCG GTGCTTGTTA TGCTTTCAAG GCTTGAGAGT 
AACCCAGATA TAATGCTATT GGCTATAAGG GCTTTAACTT ATCTGTGTGA TGTGTTCCCC 
CGAGCATCCG TTTTTCTTGT TAAGCATAAT GCAATTCCTG CTATTTGTCA AAGATTAATG 
GCCATTGAAT ACTTGGATGT AGCTGAACAG TGCTTGCAAG CATTGGAGAA AATATCTCGC 
GATCAACCAC TTCCATGCTT ACAAGCTGGG GCAATAATGG CAGTTCTAAG TTTTGTAGAC 
TTCTTCTCTA CAAGTGTACA AAGAGTTGCA CTTTCAACTG TGGTGAACAT ATGCAAGAAA 
CTGCCATCAG AAAATTTTTC ACCATTCATG GAGGCTGTTC CGAGATTATG CAATCTTCTA 
CAATATGAGG ATAGGCAGCT TGTCGAAAAT GTAGCAATTT GCTTGATCAA AATAGCAGAA 
CGGGTCAGTC AGTTGTCAGA GATGCTGGAT GAACTATGCA AGCATGGACT AATTAATCAA 
GCTACTCATC TAGTGCAATT GAACAGCCGA ACAACACTAT CTCAGCCAGT ATACAATGGT 
TTGATTGGGC TTCTTGTCAA ACTTTGTTCT GGTTCAGTTG TCGCCTTCAA GACCCTGTAT 
GAGCTCAATA TAAGCAGCAT TTTGAAGGAC ATATTAACTA CTTATGACCT CTCGCATGGA 
ATGTCTTCTC CACACGTGGT TGATGGGCAG GGTAACCAGG TACATGAAGT GCTAAAGTTG 
CTAAATGTGC TTCTCCCTAT TATAGCAAGA GATCAAGACG TTCAACAGCA TGTGTTGGAT 
AAAGAGACAT TTTTGGCTAA TCACCCTAAG CTCTTGCAGA AGTTTGGATT GGATATAATT 
CCCTCATTGA TTCAGGTGGT TAATTCTGGT GCAAATCTAT ATGTTTGCTA TGGCTGCTTA 
TGTGTTATCA ACAAGTTGGT TTATCTCAGC AAATCTGACA TGCTTCTTGA GTTGCTTAAG 
AATACAAACA TTCCAAGTTT TTTGGCTGGA GTTCTTACCC GTAAGGATCA CCATGTGCTG 
ATGTTAGCCC TGCAAATTAC TGAGACAATT CTCCAAAAGC TTCCAGATGT TTTTGTGAAC 
TCTTTCATCA AGGAAGGTGT CTTTTTTGCC ATTGATGCCC TTCTCGTGTC AGAAAAATGT 
TCTCAATTGA TGTTCCCGGT GTGCAGTGGC ATTCAGCTTC CAATCGATGC TAGTCTAAAA 
TCTTCCTCAA AAGTAGTCAT GAGATGTTTA TGTTATGCTT TTGATACTGG TCAGTCTCTT 
TCAACCACAG AAACAGGAAC ATGCAAGCTT GAAAAGGACA GTGTTCAAAA TCTTGCAAAG 
CACATCAGAA CTAGTTGCTT TGCTTCAGAA TTATGCAACT CTGAGAAAGG ATTGACAGAT 
ATCCTTCAGA AGCTTAGGGC TCTTTCAGCT GAATTGAGTG ATTTGATGAA TATGCCTGGC 
AATATTGGCT CTTGCACTCA GGATGAGGAG AAATGTTATT GTGTGTTGCG TCAAATCATT 
GAAAAGCTTG ATGGAAGAGA ACCTGTGTCT ACTTTTGAAT TCATTGAAAG TGGGATTGTG 
AAGATATTAG TGAATTACTT GTCCAGTGGC AAATATCTGA GAGAAAAGGT GGAGCCTCAG 
GGTACATTGG ATGATTGCGA CGTTATAGAG AAAAGATTTG AGGTGTTTGC AAGGCTACTT 
TTATCTTCCC CAGACCTATC TGTAGAGTTT CCTTTATCAG TTTTAATACA GAAATTGCAA 
GGTGCATTAT CTTCTTTGGA GAATTTCCCT GTTATTTTGA GCCATGCATC CAAGCAAAGA 
TCCTCGTTTG CAATCATTCC TACTGGACAT TGCACATCTT ATCCGTGCCT AAGAGTACGT 
TTTGTCAGGG GAAAGGGTGA GACATGCCTC TGCGACTACT CTGAAGATGT TGTGACAGTA 
GACCCTCTTT CTTCTGTGGA TGCAATTGAA GGATATTTAT CGCCTAAAGT TAGAATAAAA 
GGAACAGAGC AAATAGAATC AGCTGCTCAA GCTATAGAAG GCGCGTTGTC AGCAGAAAAT 
GCACAGTTCA AATCACCATC AACTGCAAAC TCCAGTCAAG GGGAAAGTTC AGGACTCATG 
GAGCCTGATA GTATTGCTAC TGATTTGCCT GAAGATGAAG CTAACCTATC TCAGTCTCCA 
CCAGAACCAG ATGTAAATTT GTTGCAGAGA AATCCTGATG AAACAACATC CTCAAATAAT 
ACTCATAATG TCTCAGTGGA GAAAATAGTG CAGTCTCCTT CATGTGCTGA TGTCACTACA 
AAAGGTCATT GTCTTATGTC CTGTAGCAAT GGAGATGCTC TGCCGAAACT GGTATTTTAC 
CTAGAAGGGC AGCGGCTGGA CCAAACTTTG ACTCTTTACC AGGCAATTCT CCAGCAAAAA 
GTTAAAGCAG ACCGTGAAAT TAACAGCACT GCAAAGCTGT GGACTCAAGT ACACACCTTG 
ACTTACGGAA TGGTTGTGGA CCCTAAAGAT GATAGTCCTC CAGATCATTC TAGTACAGCT 
CAAAATTCCT CTATGTTAGA TCAAGTTGGG GCTTATATGC AGCATCCTGC ATTTTTCTCA 
AGCTTGTTTA ATGGCGAACT CACTTCTGAC CTGGATAAGT ATAGTCCTAC TAATGATGTA 
TTGTTTCTGC TTAAAAGTTT AGAAGGTTTA AACAGGTTTA TATTTCATCT GATGTCTCGT 
GAAAGAATCC ATGCATTTGC TGAAGGGCTG ATTGATAATT TGGGTTATTT AAAGGTAGCA 
GTTCGCCCAG TATCACAAAA TGAATTTGTG AGCTGTAAGT TGACCGAGAA ATTGGAGCAA 
CAGATGCGGG ATTCTCTGGC TGTGTCCATA GGTGGCATGC CTGTGTGGTG CAATCAGCTC 
ATGGATTCAT GCTCCTTTTT GTTTAGCTTT GAGGCTAGAT GTAAATATTT CCGCTTGTCA 
GCATTCGGCC GTCAGCAAGT TCAACCACAG CCATCATCAC ATAATAATTC AGGTGTCTCA 
AGGGACGGAC CACCAAGTGC TGGCAGTTTG TCCCGTAAGA AATTCCTAGT TTTGCGTGAC 
CGAGTTCTGG AGTCTGCTGC CCAGATGATG GATAGTTATG CTCATGTTAA AGCACCTATT 
GAAGTGGAAT ATAATGAAGA AGTTGGTACT GGTCTCGGTC CAACCTTGGA ATTTTATACT 
CTGGTTAGTC GTGAGTTTCA GAAGTCTGGC CTTGGTATGT GGAGGCAGGA TCATATTTCA 
TTTACCACCA GCGAGACCCT GCAGGCTGAA TACTCTGGAA TTGTGAACTC TTCATTTGGA 
CTTTTTCCTC GCCCATGGCC GTCTTCAGTG GATGCATCTG ATGCGGCACA GTTTTCTGAA 
GTTATTAAAA AGTTTTTCCT TCTGGGACAA ATTGTAGCCA AGGCTCTTCA AGATGGGAGG 
GTGCTGGATC TACCATTCTC TAAAGCCTTC TATAAGCTTA TTCTTCAGCA GGAACTTAAT 
TTGTATGACA TCCAGTCCTT TGATCCCGAG CTTGGTAGGA CATTGCTAGA GTTCCAGGCT 
CTTGTCAACA GGAAAAAAAA TATGGGATCA GCCTTTGGAG AAAACTCATC TTCTGCTCTA 
GATGCATGCT TTTGGAACAC CAAAATCGAG GATCTTTATC TTGACTTTAC TCTTCCTGGG 
TATCCTGATT ACGTTCTTAG TTTTGATGAA GATCACAAAA TTGTGAATAT GGTTAACTTG 
GATGCTTATG TTTCTCGCAT TGTGGATGCT ACTATACACA CAGGAATTTC CAGACAAGTT 
GAAGCATTCA AGTCTGGATT TAACCAGGTT TTTCCTATTA AACATCTTAT GATTTTCACC 
GAGGAGGAAC TTGAGCGTTT GCTATGTGGA GAACGTGAAT TTTGGGCTTT CAATGAGCTT 
TTGGACCATA TCAAGTTTGA TCATGGATAC ACTGCTAGCA GCCCTCCTGT TGTTAATTTG 
CTGGAAATCA TAAAGGAATT CGAATACGAA CAACTACGAT CATTTCTACA GTTTGTAACC 
GGGGCACCTC GGCTACCTAC AGGAGGCTTG GCATCTCTGA ATCCAAAATT GACCATTGTC 
CGGAAGCATT GTAGCAACTG TGCGGATGCG GACCTTCCCA GTGTGATGAC TTGTGCCAAT 
TATCTGAAGT TGCCACCTTA CTCGTCAAAA GACAAGATGA AAGAGAAACT TCTGTATGCC 
ATAACAGAGG GTCAAGGATC ATTCCACCTC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0006s13410g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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