LOC18096703 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18096703
Entrez Gene ID	18096703
Full Name	embryo defective 14 family protein
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006385243.1	XP_006385305.1	embryo defective 14 family protein
XM_024596412.1	XP_024452180.1	pre-mRNA-processing-splicing factor 8A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18096703 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18096703 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa08800	XM_006385243.1 Latest version!	Populus trichocarpa embryo defective 14 family protein (POPTR_0003s02640g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPa425555	XM_024596412.1 Latest version!	Populus trichocarpa pre-mRNA-processing-splicing factor 8A (LOC18096703), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18096703 ( XM_006385243.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006385305.1 Populus trichocarpa embryo defective 14 family protein (POPTR_0003s02640g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa08800
Clone ID Related Accession (Same CDS sequence)	XM_006385243.1
Accession Version	XM_006385243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	embryo defective 14 family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008469).

ATGTGGAACG ACACCGGAGA GCAGCATATA GCGCCTCCGG GGACGGCTGG GCCGTCGATC 
CCACCGCCTC CACCGTCGCA GCCTTCTTAC ACCGTACTGG CGCCGTCGCA AACGGTGTCG 
AATCCAGCGG ATGCGGAGGC GAAGCTGGAG GAGAAGGCCA GGAAATGGCA GCAACTTAAC 
ACGAAGAGGT ACAGTGATAA GCGGAAGTTT GGTTTCGTCG AGACGCAAAA GGAGGATATG 
CCGCCGGAAC ACGTGAGGAA GATTATAAGG GACCATGGTG ATATGTCCTC GAAGAAATAT 
CGCCATGATA AACGTGTGTA TCTTGGAGCC CTTAAATTCA TTCCACATGC AGTATACAAG 
CTTCTTGAGA ATATGCCAAT GCCCTGGGAG CAGGTTCGTG ATGTGAAGGT TCTATACCAT 
ATTACAGGAG CGATCACTTT TGTGAATGAA ATTCCATGGG TTGTTGAACC TATTTACTTA 
GCTCAGTGGG GGACAATGTG GATCATGATG CGAAGAGAAA AGAGGGATCG GCGGCATTTC 
AAGAGAATGA GGTTTCCGCC TTTTGATGAT GAGGAACCTC CTTTAGACTA TGCTGATAAT 
TTGTTAGATG TTGATCCTCT AGAGCCTATT CAATTAGAGT TGGACGAAGA AGAAGATTCT 
GCTGTCTATA CTTGGTTTTA TGACCATAAG CCTCTTGTTA AAACGAAGCT TATCAATGGT 
CCTAGTTACA GGAAGTGGCA TCTCTCTCTT CCAATAATGG CGACTCTTCA CCGTCTGGCA 
GGACAGCTTC TTTCTGATCT AATTGACCGC AACTATTTTT ACTTGTTCGA CATGGAGTCA 
TTCTTTACAG CCAAAGCATT GAACATGTGC ATACCTGGCG GGCCTAAGTT TGAACCTTTG 
TATCGTGACA TGGAGAAAGG TGATGAGGAC TGGAACGAAT TCAATGACAT CAACAAACTC 
ATTATTCGGT CACCTTTGCG GACAGAGTAT AGAATTGCAT TTCCTCATCT TTACAATAAT 
AGGCCCCGGA AAGTAAAACT TTGTGTGTAT CACACTCCCA TGATCATGTA CATTAAAGCT 
GAGGATCCTG ATTTACCTGC TTTCTATTAT GATCCTTTGA TACACCCAAT AACCTCCTCT 
AACAAGGAAC GCCGTGAAAA AAAGACTCAT GATGACGATG ATGATGATGA GGATTTTGTT 
ATGCCAGAAG GGGTGGAACC TTTTTTGGAG GATACACAGC TTTATACTGA CACCACAGCT 
GCTGGCATTT CCTTGTTATT TGCTAACCGA CCTTTTAACA TGAGATCTGG ACGGATGCGA 
CGGGCAGAAG ATATACCCCT TGTGTCGGAG TGGTATAAGG AGCACTGTCC TCCATCATAT 
CCTGTCAAAG TTCGAGTTAG CTATCAGAAA TTGTTGAAAT GTTTTGTTTT GAATGAACTG 
CATCATAGAC CGCCAAAAGC TCAGAAGAAG AAGCACTTGT TCCGGTCATT AGCAGCAACT 
AAATTCTTCC AAACCACAGA GCTAGATTGG GCTGAAGCAG GTCTCCAAGT TTGTAAGCAG 
GGGTACAACA TGCTGAATCT ATTGATACAC CGGAAAAATT TAAATTACCT CCACCTTGAT 
TATAATTTTA ACTTGAAGCC TGTGAAGACC CTTACGACAA AGGAACGCAA GAAGTCACGG 
TTTGGGAATG CTTTTCATCT TTGTCGTGAG ATCTTGCGTT TGACAAAGCT TGTGGTTGAT 
GCCAATATAC AATTCAGGTT AGGTAATGTG GATGCTTTCC AATTGGCTGA CGGTCTTCAG 
TATACATTTT CCCATGTTGG TCAGTTGACG GGAATGTACA GATACAAGTA TAGGCTGATG 
CGCCAGATAA GAATGTGCAA AGATCTTAAG CATTTGATAT ATTACCGCTT CAATACTGGC 
CCAGTGGGTA AGGGACCTGG TTGTGGATTT TGGGCACCAA TGTGGAGGGT ATGGCTATTT 
TTCCTACGTG GCATAGTGCC TCTTCTTGAA CGGTGGTTGG GTAATCTTCT TGCAAGACAG 
TTTGAAGGGC GCCATTCAAA AGGAACTGCC AAGACTGTTA CAAAGCAGCG TGTCGAGAGC 
CACTTTGACT TGGAACTCCG TGCCGCTGTT ATGCATGATG TTCTTGATGC CATGCCAGAG 
GGCATTAAGC AAAATAAGGC TAGAACGATA CTGCAGCACC TTAGTGAGGC TTGGCGCTGC 
TGGAAAGCAA ACATTCCATG GAAGGTTCCT GGTTTACCAG TTCCCATTGA AAACATGATA 
CTTCGGTATG TTAAGTCAAA AGCAGACTGG TGGACGAATG TTGCTCACTA TAATCGTGAG 
CGTATCAGAA GGGGTGCGAC TGTTGACAAG ACAGTATGCA GAAAGAATCT TGGAAGACTG 
ACTCGGTTGT GGTTAAAGGC AGAACAGGAA CGTCAACACA ACTATTTGAA GGATGGCCCA 
TATGTCACTC CAGAAGAAGC TGTTGCGATT TACACAACTA CTGTCCATTG GTTGGAGTCC 
AGAAAGTTTT CACCAATTCC ATTCCCTCCC TTGTCATACA AGCATGACAC TAAGCTGCTT 
ATCCTTGCTT TGGAAAGGTT GAAAGAGTCA TACAGTGTGG CTGTGAGATT AAACCAACTG 
CAGAGGGAAG AACTAGGTCT TATTGAACAA GCTTATGACA ACCCCCATGA GGCTTTATCA 
CGGATCAAGC GTCACCTGCT CACTCAGCGT GCTTTCAAAG AAGTGGGCAT AGAGTTCATG 
GATTTGTACA GTTCTCTGAT TCCGGTTTAT GAAATTGAGC CTCTTGAAAA AATAACAGAT 
GCTTATTTGG ACCAATATCT GTGGTATGAA GGTGATAAGC GCCACCTCTT TCCAAACTGG 
ATTAAACCTG CAGATTCCGA GCCACCACCA CTGCTTGTCT ATAAATGGTG TCAAGGCATA 
AACAATTTGC AAGGTATCTG GGACACAAGT GAGGGGCAGT GTGTGGTGAT GCTTCAGACC 
AAGTTTGAGA AGTTCTTTGA AAAGATCGAC TTGACAATGT TAAATAGGCT TCTTCGGTTG 
GTCCTTGACC ACAATATTGC TGATTATGTG ACTGCCAAGA ACAATGTTGT CCTTTCCTAC 
AAGGACATGA GTCACACGAA CTCGTATGGT TTGATACGTG GCCTTCAGTT TGCTTCTTTT 
GTGGTGCAGT ATTATGGACT TGTGCTGGAT CTCCTGCTTC TTGGATTGAC CCGGGCCAGT 
GAGATCGCTG GTCCGCCACA AATGCCAAAT GAATTTATCA CCTACTGGGA TACAAAGGTT 
GAGACACGAC ATCCAATTCG TCTTTATTCC AGGTACATAG ACAGGGTGCA TATATTGTTT 
CGGTTTACTC ATGAAGAGGC CCGAGATCTA ATACAACGAT ATCTCACTGA GCATCCTGAT 
CCCAACAATG AAAATATGGT TGGGTATCAG AACAAGAAAT GCTGGCCTAG AGATGCAAGG 
ATGAGGCTTA TGAAACATGA TGTCAATCTT GGGAGGAGTG TTTTCTGGGA CATGAAGAAT 
CGTCTCCCTC GAAGCATCAC AACTTTAGAG TGGGAGAACA GCTTTGTTTC TGTTTACAGC 
AAGGACAACC CAAACCTGCT TTTCAGCATG TGTGGGTTTG AAGTTCGGAT ACTTCCTAAA 
ATAAGAATGA CTCAAGAAGC ATTCAGCAAT ACAAGAGATG GTGTTTGGAA TTTGCAGAAT 
GAGCAGACCA AGGAACGGAC CGCAGTTGCT TTTCTACGGG TTGATGATGA GCACATGAAA 
GTGTTTGAGA ATCGTGTGAG GCAGATTCTG ATGTCCTCAG GGTCAACTAC TTTCACAAAG 
ATTGTTAACA AATGGAATAC AGCCTTGATT GGATTGATGA CATATTTCCG TGAAGCAACT 
GTGCATACTC AAGAGCTGTT AGATTTGCTT GTAAAATGTG AAAATAAAAT TCAAACCCGT 
ATCAAGATTG GGCTAAACTC AAAAATGCCA AGCAGATTTC CTCCTGTTAT CTTCTATACA 
CCAAAAGAAA TTGGAGGACT TGGCATGTTA TCCATGGGTC ACATATTGAT CCCTCAAAGT 
GATCTTCGAT ATAGTCAGCA AACAGATGTT GGTGTAACCC ATTTCAGGAG CGGAATGAGT 
CATGAGGAGG ACCAGCTGAT TCCAAATCTT TATCGGTACA TTCAGCCATG GGAGAGTGAG 
TTCATTGACT CGCAGCGGGT TTGGGCAGAG TATGCATTGA AGAGGCAGGA AGCGCAGGCG 
CAAAATAGGC GTCTAACACT TGAAGATTTG GAAGATTCAT GGGACAGGGG AATTCCACGC 
ATTAATACTC TTTTTCAGAA AGATCGTCAC ACTTTAGCAT ATGACAAAGG GTGGAGAGTG 
CGCACAGACT TCAAGCAATA CCAAGTTTTA AAACAAAATC CTTTCTGGTG GACGCACCAG 
AGACATGATG GAAAATTGTG GAACTTGAAC AATTATCGAA CTGATGTCAT TCAAGCTCTT 
GGTGGTGTTG AAGGAATTCT TGAGCATACC TTGTTCAAAG GAACATATTT CCCAACTTGG 
GAAGGTCTCT TTTGGGAAAA GGCATCTGGC TTTGAGGAGT CCATGAAGTA TAAGAAGCTT 
ACTAATGCAC AGAGATCTGG TCTGAACCAA ATTCCTAACC GTAGATTTAC ACTTTGGTGG 
TCCCCCACAA TAAACAGGGC AAATGTTTAT GTGGGTTTTC AAGTGCAGTT AGATCTAACC 
GGCATCTTCA TGCACGGGAA GATCCCGACC TTGAAGATTT CGCTGATTCA GATATTCCGT 
GCTCACTTGT GGCAGAAGAT TCATGAGAGT GTTGTTATGG ACCTCTGTCA GGTCTTGGAT 
CAGGAACTGG ATGCTTTGGA AATTGAGACT GTGCAGAAAG AAACAATCCA TCCAAGGAAA 
AGTTATAAAA TGAACAGCTC CTGTGCAGAC GTTCTCCTTT TTGCTGCCCA TAGATGGCCC 
ATGTCAAAGC CTAGTCTTGT GGCTGAGTCA AAAGATGTGT TTGACCAGAA AGCAAGCAAT 
AAATACTGGA TAGATGTACA GCTGAGATGG GGAGACTATG ATTCTCATGA TATTGAGCGC 
TATACTAGGG CCAAATTTAT GGATTACACA ACAGACAACA TGTCTATATA TCCATCTCCA 
ACTGGGGTTA TGATTGGGCT TGATCTAGCG TACAATTTGC ACTCTGCATT TGGCAACTGG 
TTTCCGGGGT CTAAACCACT GCTAGCTCAA GCTATGAACA AAATAATGAA GTCAAACCCA 
GCTTTGTATG TCTTGAGGGA GCGCATAAGA AAAGGTTTGC AGTTGTATTC TTCAGAGCCT 
ACAGAACCAT ATTTATCATC ACAGAATTAT GGGGAGATCT TTAGCAATCA GATAATATGG 
TTTGTTGATG ATACTAATGT CTACCGTGTC ACAATCCACA AGACATTTGA AGGGAATCTC 
ACAACAAAAC CCATAAATGG TGCAATCTTC ATATTCAATC CGAGAACTGG GCAGCTATTT 
TTGAAGGTTA TCCACACTAG TGTATGGGCA GGACAGAAAC GTCTGGGCCA GCTTGCCAAG 
TGGAAAACTG CTGAAGAAGT TGCAGCTCTT GTGCGGTCTC TGCCTGTGGA AGAACAACCA 
AAACAAATTA TTGTGACTCG CAAAGGGATG CTTGATCCAT TGGAGGTTCA CTTGCTTGAT 
TTCCCCAACA TAGTTATCAA GGGAAGTGAG TTGCAGCTCC CATTCCAGTC ATGCTTAAAG 
ATAGAGAAAT TTGGTGATTT GATTTTGAAG GCAACGGAAC CACAAATGGT TTTGTTCAAT 
ATTTATGATG ATTGGCTGAA GAGTATATCA TCTTACACTG CGTTCTCTCG GCTCATTCTG 
ATTCTCCGTG CACTTCATGT TAATAATGAG AAGGCAAAGA TGCTACTCAA GCCTGACAAG 
ACTATCGTTA CTGAGCCACA CCACATCTGG CCTTCACTTA CTGATGATCA GTGGATGAAA 
GTTGAGGTTG CTTTAAGGGA TCTCATACTC TCTGACTATG CCAAGAAGAA CAATGTGAAC 
ACCTCAGCAC TGACACAATC TGAGATACGT GATATTATTC TTGGAGCTGA GATCACTCCT 
CCTTCTCAAC AAAGGCAGCA GATAGCAGAG ATTGAGAAAC AGGCCAAAGA AGCGAGTCAA 
CTGACAGCGG TCACCACAAG GACCACAAAT GTGCATGGTG ATGAACTCAT TGTCACAACC 
ACTAGTCCAT ATGAGCAAGC TGCGTTTGGT TCTAAAACAG ATTGGCGTGT CAGAGCTATA 
TCTGCTACAA ATCTCTATCT TCGAGTCAAT CATATCTATG TGAATTCTGA GGATATCAAG 
GAAACTGGAT ACACATATAT CATGCCGAAG AATATCTTGA AGAAGTTTAT CTGTATAGCT 
GATCTGCGAA CTCAAATTTC TGGGTATTTG TATGGTATAA GCCCCCCGGA CAATCCTCAG 
GTCAAGGAAA TCCGTTGTAT TGCTATGCCC CCACAGTGGG GGACTCATCA ACAGGTTCAT 
CTTCCATCAG CTCTTCCTGA GCATGATTTC CTCAATGATC TGGAGCCTTT GGGATGGATG 
CACACACAAC CAAATGAGCT TCCTCAGTTA TCACCACAGG ATCTCACGGC TCATGCTCGG 
GTTTTGGAGA ACAACAAGCA ATGGGATGGA GAAAAGTGCA TCATCCTGAC ATGTAGTTTC 
ACTCCAGGAT CATGCTCGTT AACTGCATAT AAGCTTACTC CTAGTGGTTA TGAGTGGGGA 
CGTGTCAACA AGGACACGGG AAGCAATCCT CATGGCTACC TGCCAACTCA TTACGAGAAG 
GTTCAGATGC TCCTGAGCGA CCGCTTCCTT GGTTTCTACA TGATTCCTGA TAATGGGCCA 
TGGAATTACA ATTTTATGGG AGTGAAGCAT ACAGTGAGCA TGAAATATGG AATCAAGCTG 
GGGACACCTC GAGAGTATTA CCATGAGGAC CATAGGCCAA CACATTTCCT GGAGTTCAGC 
AACCTGGAGG AGGGCGAGAC CGCTGAAGGC GATAGAGAGG ATACATTCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006385305.1
CDS	65..7138
Translation	MWNDTGEQHIAPPGTAGPSIPPPPPSQPSYTVLAPSQTVSNPADAEAKLEEKARKWQQLNTKRYSDKRKFGFVETQKEDMPPEHVRKIIRDHGDMSSKKYRHDKRVYLGALKFIPHAVYKLLENMPMPWEQVRDVKVLYHITGAITFVNEIPWVVEPIYLAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNLLDVDPLEPIQLELDEEEDSAVYTWFYDHKPLVKTKLINGPSYRKWHLSLPIMATLHRLAGQLLSDLIDRNYFYLFDMESFFTAKALNMCIPGGPKFEPLYRDMEKGDEDWNEFNDINKLIIRSPLRTEYRIAFPHLYNNRPRKVKLCVYHTPMIMYIKAEDPDLPAFYYDPLIHPITSSNKERREKKTHDDDDDDEDFVMPEGVEPFLEDTQLYTDTTAAGISLLFANRPFNMRSGRMRRAEDIPLVSEWYKEHCPPSYPVKVRVSYQKLLKCFVLNELHHRPPKAQKKKHLFRSLAATKFFQTTELDWAEAGLQVCKQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDANIQFRLGNVDAFQLADGLQYTFSHVGQLTGMYRYKYRLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRHSKGTAKTVTKQRVESHFDLELRAAVMHDVLDAMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPVPIENMILRYVKSKADWWTNVAHYNRERIRRGATVDKTVCRKNLGRLTRLWLKAEQERQHNYLKDGPYVTPEEAVAIYTTTVHWLESRKFSPIPFPPLSYKHDTKLLILALERLKESYSVAVRLNQLQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSSLIPVYEIEPLEKITDAYLDQYLWYEGDKRHLFPNWIKPADSEPPPLLVYKWCQGINNLQGIWDTSEGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSYGLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLYSRYIDRVHILFRFTHEEARDLIQRYLTEHPDPNNENMVGYQNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGVWNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVIFYTPKEIGGLGMLSMGHILIPQSDLRYSQQTDVGVTHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAQAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDVIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVLDQELDALEIETVQKETIHPRKSYKMNSSCADVLLFAAHRWPMSKPSLVAESKDVFDQKASNKYWIDVQLRWGDYDSHDIERYTRAKFMDYTTDNMSIYPSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEVAALVRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQSCLKIEKFGDLILKATEPQMVLFNIYDDWLKSISSYTAFSRLILILRALHVNNEKAKMLLKPDKTIVTEPHHIWPSLTDDQWMKVEVALRDLILSDYAKKNNVNTSALTQSEIRDIILGAEITPPSQQRQQIAEIEKQAKEASQLTAVTTRTTNVHGDELIVTTTSPYEQAAFGSKTDWRVRAISATNLYLRVNHIYVNSEDIKETGYTYIMPKNILKKFICIADLRTQISGYLYGISPPDNPQVKEIRCIAMPPQWGTHQQVHLPSALPEHDFLNDLEPLGWMHTQPNELPQLSPQDLTAHARVLENNKQWDGEKCIILTCSFTPGSCSLTAYKLTPSGYEWGRVNKDTGSNPHGYLPTHYEKVQMLLSDRFLGFYMIPDNGPWNYNFMGVKHTVSMKYGIKLGTPREYYHEDHRPTHFLEFSNLEEGETAEGDREDTFT

Target ORF information:

RefSeq Version	XM_006385243.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa embryo defective 14 family protein (POPTR_0003s02640g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006385243.1

ORF Insert Sequence:

ATGTGGAACG ACACCGGAGA GCAGCATATA GCGCCTCCGG GGACGGCTGG GCCGTCGATC 
CCACCGCCTC CACCGTCGCA GCCTTCTTAC ACCGTACTGG CGCCGTCGCA AACGGTGTCG 
AATCCAGCGG ATGCGGAGGC GAAGCTGGAG GAGAAGGCCA GGAAATGGCA GCAACTTAAC 
ACGAAGAGGT ACAGTGATAA GCGGAAGTTT GGTTTCGTCG AGACGCAAAA GGAGGATATG 
CCGCCGGAAC ACGTGAGGAA GATTATAAGG GACCATGGTG ATATGTCCTC GAAGAAATAT 
CGCCATGATA AACGTGTGTA TCTTGGAGCC CTTAAATTCA TTCCACATGC AGTATACAAG 
CTTCTTGAGA ATATGCCAAT GCCCTGGGAG CAGGTTCGTG ATGTGAAGGT TCTATACCAT 
ATTACAGGAG CGATCACTTT TGTGAATGAA ATTCCATGGG TTGTTGAACC TATTTACTTA 
GCTCAGTGGG GGACAATGTG GATCATGATG CGAAGAGAAA AGAGGGATCG GCGGCATTTC 
AAGAGAATGA GGTTTCCGCC TTTTGATGAT GAGGAACCTC CTTTAGACTA TGCTGATAAT 
TTGTTAGATG TTGATCCTCT AGAGCCTATT CAATTAGAGT TGGACGAAGA AGAAGATTCT 
GCTGTCTATA CTTGGTTTTA TGACCATAAG CCTCTTGTTA AAACGAAGCT TATCAATGGT 
CCTAGTTACA GGAAGTGGCA TCTCTCTCTT CCAATAATGG CGACTCTTCA CCGTCTGGCA 
GGACAGCTTC TTTCTGATCT AATTGACCGC AACTATTTTT ACTTGTTCGA CATGGAGTCA 
TTCTTTACAG CCAAAGCATT GAACATGTGC ATACCTGGCG GGCCTAAGTT TGAACCTTTG 
TATCGTGACA TGGAGAAAGG TGATGAGGAC TGGAACGAAT TCAATGACAT CAACAAACTC 
ATTATTCGGT CACCTTTGCG GACAGAGTAT AGAATTGCAT TTCCTCATCT TTACAATAAT 
AGGCCCCGGA AAGTAAAACT TTGTGTGTAT CACACTCCCA TGATCATGTA CATTAAAGCT 
GAGGATCCTG ATTTACCTGC TTTCTATTAT GATCCTTTGA TACACCCAAT AACCTCCTCT 
AACAAGGAAC GCCGTGAAAA AAAGACTCAT GATGACGATG ATGATGATGA GGATTTTGTT 
ATGCCAGAAG GGGTGGAACC TTTTTTGGAG GATACACAGC TTTATACTGA CACCACAGCT 
GCTGGCATTT CCTTGTTATT TGCTAACCGA CCTTTTAACA TGAGATCTGG ACGGATGCGA 
CGGGCAGAAG ATATACCCCT TGTGTCGGAG TGGTATAAGG AGCACTGTCC TCCATCATAT 
CCTGTCAAAG TTCGAGTTAG CTATCAGAAA TTGTTGAAAT GTTTTGTTTT GAATGAACTG 
CATCATAGAC CGCCAAAAGC TCAGAAGAAG AAGCACTTGT TCCGGTCATT AGCAGCAACT 
AAATTCTTCC AAACCACAGA GCTAGATTGG GCTGAAGCAG GTCTCCAAGT TTGTAAGCAG 
GGGTACAACA TGCTGAATCT ATTGATACAC CGGAAAAATT TAAATTACCT CCACCTTGAT 
TATAATTTTA ACTTGAAGCC TGTGAAGACC CTTACGACAA AGGAACGCAA GAAGTCACGG 
TTTGGGAATG CTTTTCATCT TTGTCGTGAG ATCTTGCGTT TGACAAAGCT TGTGGTTGAT 
GCCAATATAC AATTCAGGTT AGGTAATGTG GATGCTTTCC AATTGGCTGA CGGTCTTCAG 
TATACATTTT CCCATGTTGG TCAGTTGACG GGAATGTACA GATACAAGTA TAGGCTGATG 
CGCCAGATAA GAATGTGCAA AGATCTTAAG CATTTGATAT ATTACCGCTT CAATACTGGC 
CCAGTGGGTA AGGGACCTGG TTGTGGATTT TGGGCACCAA TGTGGAGGGT ATGGCTATTT 
TTCCTACGTG GCATAGTGCC TCTTCTTGAA CGGTGGTTGG GTAATCTTCT TGCAAGACAG 
TTTGAAGGGC GCCATTCAAA AGGAACTGCC AAGACTGTTA CAAAGCAGCG TGTCGAGAGC 
CACTTTGACT TGGAACTCCG TGCCGCTGTT ATGCATGATG TTCTTGATGC CATGCCAGAG 
GGCATTAAGC AAAATAAGGC TAGAACGATA CTGCAGCACC TTAGTGAGGC TTGGCGCTGC 
TGGAAAGCAA ACATTCCATG GAAGGTTCCT GGTTTACCAG TTCCCATTGA AAACATGATA 
CTTCGGTATG TTAAGTCAAA AGCAGACTGG TGGACGAATG TTGCTCACTA TAATCGTGAG 
CGTATCAGAA GGGGTGCGAC TGTTGACAAG ACAGTATGCA GAAAGAATCT TGGAAGACTG 
ACTCGGTTGT GGTTAAAGGC AGAACAGGAA CGTCAACACA ACTATTTGAA GGATGGCCCA 
TATGTCACTC CAGAAGAAGC TGTTGCGATT TACACAACTA CTGTCCATTG GTTGGAGTCC 
AGAAAGTTTT CACCAATTCC ATTCCCTCCC TTGTCATACA AGCATGACAC TAAGCTGCTT 
ATCCTTGCTT TGGAAAGGTT GAAAGAGTCA TACAGTGTGG CTGTGAGATT AAACCAACTG 
CAGAGGGAAG AACTAGGTCT TATTGAACAA GCTTATGACA ACCCCCATGA GGCTTTATCA 
CGGATCAAGC GTCACCTGCT CACTCAGCGT GCTTTCAAAG AAGTGGGCAT AGAGTTCATG 
GATTTGTACA GTTCTCTGAT TCCGGTTTAT GAAATTGAGC CTCTTGAAAA AATAACAGAT 
GCTTATTTGG ACCAATATCT GTGGTATGAA GGTGATAAGC GCCACCTCTT TCCAAACTGG 
ATTAAACCTG CAGATTCCGA GCCACCACCA CTGCTTGTCT ATAAATGGTG TCAAGGCATA 
AACAATTTGC AAGGTATCTG GGACACAAGT GAGGGGCAGT GTGTGGTGAT GCTTCAGACC 
AAGTTTGAGA AGTTCTTTGA AAAGATCGAC TTGACAATGT TAAATAGGCT TCTTCGGTTG 
GTCCTTGACC ACAATATTGC TGATTATGTG ACTGCCAAGA ACAATGTTGT CCTTTCCTAC 
AAGGACATGA GTCACACGAA CTCGTATGGT TTGATACGTG GCCTTCAGTT TGCTTCTTTT 
GTGGTGCAGT ATTATGGACT TGTGCTGGAT CTCCTGCTTC TTGGATTGAC CCGGGCCAGT 
GAGATCGCTG GTCCGCCACA AATGCCAAAT GAATTTATCA CCTACTGGGA TACAAAGGTT 
GAGACACGAC ATCCAATTCG TCTTTATTCC AGGTACATAG ACAGGGTGCA TATATTGTTT 
CGGTTTACTC ATGAAGAGGC CCGAGATCTA ATACAACGAT ATCTCACTGA GCATCCTGAT 
CCCAACAATG AAAATATGGT TGGGTATCAG AACAAGAAAT GCTGGCCTAG AGATGCAAGG 
ATGAGGCTTA TGAAACATGA TGTCAATCTT GGGAGGAGTG TTTTCTGGGA CATGAAGAAT 
CGTCTCCCTC GAAGCATCAC AACTTTAGAG TGGGAGAACA GCTTTGTTTC TGTTTACAGC 
AAGGACAACC CAAACCTGCT TTTCAGCATG TGTGGGTTTG AAGTTCGGAT ACTTCCTAAA 
ATAAGAATGA CTCAAGAAGC ATTCAGCAAT ACAAGAGATG GTGTTTGGAA TTTGCAGAAT 
GAGCAGACCA AGGAACGGAC CGCAGTTGCT TTTCTACGGG TTGATGATGA GCACATGAAA 
GTGTTTGAGA ATCGTGTGAG GCAGATTCTG ATGTCCTCAG GGTCAACTAC TTTCACAAAG 
ATTGTTAACA AATGGAATAC AGCCTTGATT GGATTGATGA CATATTTCCG TGAAGCAACT 
GTGCATACTC AAGAGCTGTT AGATTTGCTT GTAAAATGTG AAAATAAAAT TCAAACCCGT 
ATCAAGATTG GGCTAAACTC AAAAATGCCA AGCAGATTTC CTCCTGTTAT CTTCTATACA 
CCAAAAGAAA TTGGAGGACT TGGCATGTTA TCCATGGGTC ACATATTGAT CCCTCAAAGT 
GATCTTCGAT ATAGTCAGCA AACAGATGTT GGTGTAACCC ATTTCAGGAG CGGAATGAGT 
CATGAGGAGG ACCAGCTGAT TCCAAATCTT TATCGGTACA TTCAGCCATG GGAGAGTGAG 
TTCATTGACT CGCAGCGGGT TTGGGCAGAG TATGCATTGA AGAGGCAGGA AGCGCAGGCG 
CAAAATAGGC GTCTAACACT TGAAGATTTG GAAGATTCAT GGGACAGGGG AATTCCACGC 
ATTAATACTC TTTTTCAGAA AGATCGTCAC ACTTTAGCAT ATGACAAAGG GTGGAGAGTG 
CGCACAGACT TCAAGCAATA CCAAGTTTTA AAACAAAATC CTTTCTGGTG GACGCACCAG 
AGACATGATG GAAAATTGTG GAACTTGAAC AATTATCGAA CTGATGTCAT TCAAGCTCTT 
GGTGGTGTTG AAGGAATTCT TGAGCATACC TTGTTCAAAG GAACATATTT CCCAACTTGG 
GAAGGTCTCT TTTGGGAAAA GGCATCTGGC TTTGAGGAGT CCATGAAGTA TAAGAAGCTT 
ACTAATGCAC AGAGATCTGG TCTGAACCAA ATTCCTAACC GTAGATTTAC ACTTTGGTGG 
TCCCCCACAA TAAACAGGGC AAATGTTTAT GTGGGTTTTC AAGTGCAGTT AGATCTAACC 
GGCATCTTCA TGCACGGGAA GATCCCGACC TTGAAGATTT CGCTGATTCA GATATTCCGT 
GCTCACTTGT GGCAGAAGAT TCATGAGAGT GTTGTTATGG ACCTCTGTCA GGTCTTGGAT 
CAGGAACTGG ATGCTTTGGA AATTGAGACT GTGCAGAAAG AAACAATCCA TCCAAGGAAA 
AGTTATAAAA TGAACAGCTC CTGTGCAGAC GTTCTCCTTT TTGCTGCCCA TAGATGGCCC 
ATGTCAAAGC CTAGTCTTGT GGCTGAGTCA AAAGATGTGT TTGACCAGAA AGCAAGCAAT 
AAATACTGGA TAGATGTACA GCTGAGATGG GGAGACTATG ATTCTCATGA TATTGAGCGC 
TATACTAGGG CCAAATTTAT GGATTACACA ACAGACAACA TGTCTATATA TCCATCTCCA 
ACTGGGGTTA TGATTGGGCT TGATCTAGCG TACAATTTGC ACTCTGCATT TGGCAACTGG 
TTTCCGGGGT CTAAACCACT GCTAGCTCAA GCTATGAACA AAATAATGAA GTCAAACCCA 
GCTTTGTATG TCTTGAGGGA GCGCATAAGA AAAGGTTTGC AGTTGTATTC TTCAGAGCCT 
ACAGAACCAT ATTTATCATC ACAGAATTAT GGGGAGATCT TTAGCAATCA GATAATATGG 
TTTGTTGATG ATACTAATGT CTACCGTGTC ACAATCCACA AGACATTTGA AGGGAATCTC 
ACAACAAAAC CCATAAATGG TGCAATCTTC ATATTCAATC CGAGAACTGG GCAGCTATTT 
TTGAAGGTTA TCCACACTAG TGTATGGGCA GGACAGAAAC GTCTGGGCCA GCTTGCCAAG 
TGGAAAACTG CTGAAGAAGT TGCAGCTCTT GTGCGGTCTC TGCCTGTGGA AGAACAACCA 
AAACAAATTA TTGTGACTCG CAAAGGGATG CTTGATCCAT TGGAGGTTCA CTTGCTTGAT 
TTCCCCAACA TAGTTATCAA GGGAAGTGAG TTGCAGCTCC CATTCCAGTC ATGCTTAAAG 
ATAGAGAAAT TTGGTGATTT GATTTTGAAG GCAACGGAAC CACAAATGGT TTTGTTCAAT 
ATTTATGATG ATTGGCTGAA GAGTATATCA TCTTACACTG CGTTCTCTCG GCTCATTCTG 
ATTCTCCGTG CACTTCATGT TAATAATGAG AAGGCAAAGA TGCTACTCAA GCCTGACAAG 
ACTATCGTTA CTGAGCCACA CCACATCTGG CCTTCACTTA CTGATGATCA GTGGATGAAA 
GTTGAGGTTG CTTTAAGGGA TCTCATACTC TCTGACTATG CCAAGAAGAA CAATGTGAAC 
ACCTCAGCAC TGACACAATC TGAGATACGT GATATTATTC TTGGAGCTGA GATCACTCCT 
CCTTCTCAAC AAAGGCAGCA GATAGCAGAG ATTGAGAAAC AGGCCAAAGA AGCGAGTCAA 
CTGACAGCGG TCACCACAAG GACCACAAAT GTGCATGGTG ATGAACTCAT TGTCACAACC 
ACTAGTCCAT ATGAGCAAGC TGCGTTTGGT TCTAAAACAG ATTGGCGTGT CAGAGCTATA 
TCTGCTACAA ATCTCTATCT TCGAGTCAAT CATATCTATG TGAATTCTGA GGATATCAAG 
GAAACTGGAT ACACATATAT CATGCCGAAG AATATCTTGA AGAAGTTTAT CTGTATAGCT 
GATCTGCGAA CTCAAATTTC TGGGTATTTG TATGGTATAA GCCCCCCGGA CAATCCTCAG 
GTCAAGGAAA TCCGTTGTAT TGCTATGCCC CCACAGTGGG GGACTCATCA ACAGGTTCAT 
CTTCCATCAG CTCTTCCTGA GCATGATTTC CTCAATGATC TGGAGCCTTT GGGATGGATG 
CACACACAAC CAAATGAGCT TCCTCAGTTA TCACCACAGG ATCTCACGGC TCATGCTCGG 
GTTTTGGAGA ACAACAAGCA ATGGGATGGA GAAAAGTGCA TCATCCTGAC ATGTAGTTTC 
ACTCCAGGAT CATGCTCGTT AACTGCATAT AAGCTTACTC CTAGTGGTTA TGAGTGGGGA 
CGTGTCAACA AGGACACGGG AAGCAATCCT CATGGCTACC TGCCAACTCA TTACGAGAAG 
GTTCAGATGC TCCTGAGCGA CCGCTTCCTT GGTTTCTACA TGATTCCTGA TAATGGGCCA 
TGGAATTACA ATTTTATGGG AGTGAAGCAT ACAGTGAGCA TGAAATATGG AATCAAGCTG 
GGGACACCTC GAGAGTATTA CCATGAGGAC CATAGGCCAA CACATTTCCT GGAGTTCAGC 
AACCTGGAGG AGGGCGAGAC CGCTGAAGGC GATAGAGAGG ATACATTCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18096703 ( XM_024596412.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024452180.1 Populus trichocarpa pre-mRNA-processing-splicing factor 8A (LOC18096703), mRNA.

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CloneID	OPa425555
Clone ID Related Accession (Same CDS sequence)	XM_024596412.1
Accession Version	XM_024596412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	pre-mRNA-processing-splicing factor 8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG ACACCGGAGA GCAGCATATA GCGCCTCCGG GGACGGCTGG GCCGTCGATC 
CCACCGCCTC CACCGTCGCA GCCTTCTTAC ACCGTACTGG CGCCGTCGCA AACGGTGTCG 
AATCCAGCGG ATGCGGAGGC GAAGCTGGAG GAGAAGGCCA GGAAATGGCA GCAACTTAAC 
ACGAAGAGGT ACAGTGATAA GCGGAAGTTT GGTTTCGTCG AGACGCAAAA GGAGGATATG 
CCGCCGGAAC ACGTGAGGAA GATTATAAGG GACCATGGTG ATATGTCCTC GAAGAAATAT 
CGCCATGATA AACGTGTGTA TCTTGGAGCC CTTAAATTCA TTCCACATGC AGTATACAAG 
CTTCTTGAGA ATATGCCAAT GCCCTGGGAG CAGGTTCGTG ATGTGAAGGT TCTATACCAT 
ATTACAGGAG CGATCACTTT TGTGAATGAA ATTCCATGGG TTGTTGAACC TATTTACTTA 
GCTCAGTGGG GGACAATGTG GATCATGATG CGAAGAGAAA AGAGGGATCG GCGGCATTTC 
AAGAGAATGA GGTTTCCGCC TTTTGATGAT GAGGAACCTC CTTTAGACTA TGCTGATAAT 
TTGTTAGATG TTGATCCTCT AGAGCCTATT CAATTAGAGT TGGACGAAGA AGAAGATTCT 
GCTGTCTATA CTTGGTTTTA TGACCATAAG CCTCTTGTTA AAACGAAGCT TATCAATGGT 
CCTAGTTACA GGAAGTGGCA TCTCTCTCTT CCAATAATGG CGACTCTTCA CCGTCTGGCA 
GGACAGCTTC TTTCTGATCT AATTGACCGC AACTATTTTT ACTTGTTCGA CATGGAGTCA 
TTCTTTACAG CCAAAGCATT GAACATGTGC ATACCTGGCG GGCCTAAGTT TGAACCTTTG 
TATCGTGACA TGGAGAAAGG TGATGAGGAC TGGAACGAAT TCAATGACAT CAACAAACTC 
ATTATTCGGT CACCTTTGCG GACAGAGTAT AGAATTGCAT TTCCTCATCT TTACAATAAT 
AGGCCCCGGA AAGTAAAACT TTGTGTGTAT CACACTCCCA TGATCATGTA CATTAAAGCT 
GAGGATCCTG ATTTACCTGC TTTCTATTAT GATCCTTTGA TACACCCAAT AACCTCCTCT 
AACAAGGAAC GCCGTGAAAA AAAGACTCAT GATGACGATG ATGATGATGA GGATTTTGTT 
ATGCCAGAAG GGGTGGAACC TTTTTTGGAG GATACACAGC TTTATACTGA CACCACAGCT 
GCTGGCATTT CCTTGTTATT TGCTAACCGA CCTTTTAACA TGAGATCTGG ACGGATGCGA 
CGGGCAGAAG ATATACCCCT TGTGTCGGAG TGGTATAAGG AGCACTGTCC TCCATCATAT 
CCTGTCAAAG TTCGAGTTAG CTATCAGAAA TTGTTGAAAT GTTTTGTTTT GAATGAACTG 
CATCATAGAC CGCCAAAAGC TCAGAAGAAG AAGCACTTGT TCCGGTCATT AGCAGCAACT 
AAATTCTTCC AAACCACAGA GCTAGATTGG GCTGAAGCAG GTCTCCAAGT TTGTAAGCAG 
GGGTACAACA TGCTGAATCT ATTGATACAC CGGAAAAATT TAAATTACCT CCACCTTGAT 
TATAATTTTA ACTTGAAGCC TGTGAAGACC CTTACGACAA AGGAACGCAA GAAGTCACGG 
TTTGGGAATG CTTTTCATCT TTGTCGTGAG ATCTTGCGTT TGACAAAGCT TGTGGTTGAT 
GCCAATATAC AATTCAGGTT AGGTAATGTG GATGCTTTCC AATTGGCTGA CGGTCTTCAG 
TATACATTTT CCCATGTTGG TCAGTTGACG GGAATGTACA GATACAAGTA TAGGCTGATG 
CGCCAGATAA GAATGTGCAA AGATCTTAAG CATTTGATAT ATTACCGCTT CAATACTGGC 
CCAGTGGGTA AGGGACCTGG TTGTGGATTT TGGGCACCAA TGTGGAGGGT ATGGCTATTT 
TTCCTACGTG GCATAGTGCC TCTTCTTGAA CGGTGGTTGG GTAATCTTCT TGCAAGACAG 
TTTGAAGGGC GCCATTCAAA AGGAACTGCC AAGACTGTTA CAAAGCAGCG TGTCGAGAGC 
CACTTTGACT TGGAACTCCG TGCCGCTGTT ATGCATGATG TTCTTGATGC CATGCCAGAG 
GGCATTAAGC AAAATAAGGC TAGAACGATA CTGCAGCACC TTAGTGAGGC TTGGCGCTGC 
TGGAAAGCAA ACATTCCATG GAAGGTTCCT GGTTTACCAG TTCCCATTGA AAACATGATA 
CTTCGGTATG TTAAGTCAAA AGCAGACTGG TGGACGAATG TTGCTCACTA TAATCGTGAG 
CGTATCAGAA GGGGTGCGAC TGTTGACAAG ACAGTATGCA GAAAGAATCT TGGAAGACTG 
ACTCGGTTGT GGTTAAAGGC AGAACAGGAA CGTCAACACA ACTATTTGAA GGATGGCCCA 
TATGTCACTC CAGAAGAAGC TGTTGCGATT TACACAACTA CTGTCCATTG GTTGGAGTCC 
AGAAAGTTTT CACCAATTCC ATTCCCTCCC TTGTCATACA AGCATGACAC TAAGCTGCTT 
ATCCTTGCTT TGGAAAGGTT GAAAGAGTCA TACAGTGTGG CTGTGAGATT AAACCAACTG 
CAGAGGGAAG AACTAGGTCT TATTGAACAA GCTTATGACA ACCCCCATGA GGCTTTATCA 
CGGATCAAGC GTCACCTGCT CACTCAGCGT GCTTTCAAAG AAGTGGGCAT AGAGTTCATG 
GATTTGTACA GTTCTCTGAT TCCGGTTTAT GAAATTGAGC CTCTTGAAAA AATAACAGAT 
GCTTATTTGG ACCAATATCT GTGGTATGAA GGTGATAAGC GCCACCTCTT TCCAAACTGG 
ATTAAACCTG CAGATTCCGA GCCACCACCA CTGCTTGTCT ATAAATGGTG TCAAGGCATA 
AACAATTTGC AAGGTATCTG GGACACAAGT GAGGGGCAGT GTGTGGTGAT GCTTCAGACC 
AAGTTTGAGA AGTTCTTTGA AAAGATCGAC TTGACAATGT TAAATAGGCT TCTTCGGTTG 
GTCCTTGACC ACAATATTGC TGATTATGTG ACTGCCAAGA ACAATGTTGT CCTTTCCTAC 
AAGGACATGA GTCACACGAA CTCGTATGGT TTGATACGTG GCCTTCAGTT TGCTTCTTTT 
GTGGTGCAGT ATTATGGACT TGTGCTGGAT CTCCTGCTTC TTGGATTGAC CCGGGCCAGT 
GAGATCGCTG GTCCGCCACA AATGCCAAAT GAATTTATCA CCTACTGGGA TACAAAGGTT 
GAGACACGAC ATCCAATTCG TCTTTATTCC AGGTACATAG ACAGGGTGCA TATATTGTTT 
CGGTTTACTC ATGAAGAGGC CCGAGATCTA ATACAACGAT ATCTCACTGA GCATCCTGAT 
CCCAACAATG AAAATATGGT TGGGTATCAG AACAAGAAAT GCTGGCCTAG AGATGCAAGG 
ATGAGGCTTA TGAAACATGA TGTCAATCTT GGGAGGAGTG TTTTCTGGGA CATGAAGAAT 
CGTCTCCCTC GAAGCATCAC AACTTTAGAG TGGGAGAACA GCTTTGTTTC TGTTTACAGC 
AAGGACAACC CAAACCTGCT TTTCAGCATG TGTGGGTTTG AAGTTCGGAT ACTTCCTAAA 
ATAAGAATGA CTCAAGAAGC ATTCAGCAAT ACAAGAGATG GTGTTTGGAA TTTGCAGAAT 
GAGCAGACCA AGGAACGGAC CGCAGTTGCT TTTCTACGGG TTGATGATGA GCACATGAAA 
GTGTTTGAGA ATCGTGTGAG GCAGATTCTG ATGTCCTCAG GGTCAACTAC TTTCACAAAG 
ATTGTTAACA AATGGAATAC AGCCTTGATT GGATTGATGA CATATTTCCG TGAAGCAACT 
GTGCATACTC AAGAGCTGTT AGATTTGCTT GTAAAATGTG AAAATAAAAT TCAAACCCGT 
ATCAAGATTG GGCTAAACTC AAAAATGCCA AGCAGATTTC CTCCTGTTAT CTTCTATACA 
CCAAAAGAAA TTGGAGGACT TGGCATGTTA TCCATGGGTC ACATATTGAT CCCTCAAAGT 
GATCTTCGAT ATAGTCAGCA AACAGATGTT GGTGTAACCC ATTTCAGGAG CGGAATGAGT 
CATGAGGAGG ACCAGCTGAT TCCAAATCTT TATCGGTACA TTCAGCCATG GGAGAGTGAG 
TTCATTGACT CGCAGCGGGT TTGGGCAGAG TATGCATTGA AGAGGCAGGA AGCGCAGGCG 
CAAAATAGGC GTCTAACACT TGAAGATTTG GAAGATTCAT GGGACAGGGG AATTCCACGC 
ATTAATACTC TTTTTCAGAA AGATCGTCAC ACTTTAGCAT ATGACAAAGG GTGGAGAGTG 
CGCACAGACT TCAAGCAATA CCAAGTTTTA AAACAAAATC CTTTCTGGTG GACGCACCAG 
AGACATGATG GAAAATTGTG GAACTTGAAC AATTATCGAA CTGATGTCAT TCAAGCTCTT 
GGTGGTGTTG AAGGAATTCT TGAGCATACC TTGTTCAAAG GAACATATTT CCCAACTTGG 
GAAGGTCTCT TTTGGGAAAA GGCATCTGGC TTTGAGGAGT CCATGAAGTA TAAGAAGCTT 
ACTAATGCAC AGAGATCTGG TCTGAACCAA ATTCCTAACC GTAGATTTAC ACTTTGGTGG 
TCCCCCACAA TAAACAGGGC AAATGTTTAT GTGGGTTTTC AAGTGCAGTT AGATCTAACC 
GGCATCTTCA TGCACGGGAA GATCCCGACC TTGAAGATTT CGCTGATTCA GATATTCCGT 
GCTCACTTGT GGCAGAAGAT TCATGAGAGT GTTGTTATGG ACCTCTGTCA GGTCTTGGAT 
CAGGAACTGG ATGCTTTGGA AATTGAGACT GTGCAGAAAG AAACAATCCA TCCAAGGAAA 
AGTTATAAAA TGAACAGCTC CTGTGCAGAC GTTCTCCTTT TTGCTGCCCA TAGATGGCCC 
ATGTCAAAGC CTAGTCTTGT GGCTGAGTCA AAAGATGTGT TTGACCAGAA AGCAAGCAAT 
AAATACTGGA TAGATGTACA GCTGAGATGG GGAGACTATG ATTCTCATGA TATTGAGCGC 
TATACTAGGG CCAAATTTAT GGATTACACA ACAGACAACA TGTCTATATA TCCATCTCCA 
ACTGGGGTTA TGATTGGGCT TGATCTAGCG TACAATTTGC ACTCTGCATT TGGCAACTGG 
TTTCCGGGGT CTAAACCACT GCTAGCTCAA GCTATGAACA AAATAATGAA GTCAAACCCA 
GCTTTGTATG TCTTGAGGGA GCGCATAAGA AAAGGTTTGC AGTTGTATTC TTCAGAGCCT 
ACAGAACCAT ATTTATCATC ACAGAATTAT GGGGAGATCT TTAGCAATCA GATAATATGG 
TTTGTTGATG ATACTAATGT CTACCGTGTC ACAATCCACA AGACATTTGA AGGGAATCTC 
ACAACAAAAC CCATAAATGG TGCAATCTTC ATATTCAATC CGAGAACTGG GCAGCTATTT 
TTGAAGGTTA TCCACACTAG TGTATGGGCA GGACAGAAAC GTCTGGGCCA GCTTGCCAAG 
TGGAAAACTG CTGAAGAAGT TGCAGCTCTT GTGCGGTCTC TGCCTGTGGA AGAACAACCA 
AAACAAATTA TTGTGACTCG CAAAGGGATG CTTGATCCAT TGGAGGTTCA CTTGCTTGAT 
TTCCCCAACA TAGTTATCAA GGGAAGTGAG TTGCAGCTCC CATTCCAGTC ATGCTTAAAG 
ATAGAGAAAT TTGGTGATTT GATTTTGAAG GCAACGGAAC CACAAATGGT TTTGTTCAAT 
ATTTATGATG ATTGGCTGAA GAGTATATCA TCTTACACTG CGTTCTCTCG GCTCATTCTG 
ATTCTCCGTG CACTTCATGT TAATAATGAG AAGGCAAAGA TGCTACTCAA GCCTGACAAG 
ACTATCGTTA CTGAGCCACA CCACATCTGG CCTTCACTTA CTGATGATCA GTGGATGAAA 
GTTGAGGTTG CTTTAAGGGA TCTCATACTC TCTGACTATG CCAAGAAGAA CAATGTGAAC 
ACCTCAGCAC TGACACAATC TGAGATACGT GATATTATTC TTGGAGCTGA GATCACTCCT 
CCTTCTCAAC AAAGGCAGCA GATAGCAGAG ATTGAGAAAC AGGCCAAAGA AGCGAGTCAA 
CTGACAGCGG TCACCACAAG GACCACAAAT GTGCATGGTG ATGAACTCAT TGTCACAACC 
ACTAGTCCAT ATGAGCAAGC TGCGTTTGGT TCTAAAACAG ATTGGCGTGT CAGAGCTATA 
TCTGCTACAA ATCTCTATCT TCGAGTCAAT CATATCTATG TGAATTCTGA GGATATCAAG 
GAAACTGGAT ACACATATAT CATGCCGAAG AATATCTTGA AGAAGTTTAT CTGTATAGCT 
GATCTGCGAA CTCAAATTTC TGGGTATTTG TATGGTATAA GCCCCCCGGA CAATCCTCAG 
GTCAAGGAAA TCCGTTGTAT TGCTATGCCC CCACAGTGGG GGACTCATCA ACAGGTTCAT 
CTTCCATCAG CTCTTCCTGA GCATGATTTC CTCAATGATC TGGAGCCTTT GGGATGGATG 
CACACACAAC CAAATGAGCT TCCTCAGTTA TCACCACAGG ATCTCACGGC TCATGCTCGG 
GTTTTGGAGA ACAACAAGCA ATGGGATGGA GAAAAGTGCA TCATCCTGAC ATGTAGTTTC 
ACTCCAGGAT CATGCTCGTT AACTGCATAT AAGCTTACTC CTAGTGGTTA TGAGTGGGGA 
CGTGTCAACA AGGACACGGG AAGCAATCCT CATGGCTACC TGCCAACTCA TTACGAGAAG 
GTTCAGATGC TCCTGAGCGA CCGCTTCCTT GGTTTCTACA TGATTCCTGA TAATGGGCCA 
TGGAATTACA ATTTTATGGG AGTGAAGCAT ACAGTGAGCA TGAAATATGG AATCAAGCTG 
GGGACACCTC GAGAGTATTA CCATGAGGAC CATAGGCCAA CACATTTCCT GGAGTTCAGC 
AACCTGGAGG AGGGTGAGAC CGCTGAAGGC GATAGAGAGG ATACATTCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024452180.1
CDS	92..7165
Translation	MWNDTGEQHIAPPGTAGPSIPPPPPSQPSYTVLAPSQTVSNPADAEAKLEEKARKWQQLNTKRYSDKRKFGFVETQKEDMPPEHVRKIIRDHGDMSSKKYRHDKRVYLGALKFIPHAVYKLLENMPMPWEQVRDVKVLYHITGAITFVNEIPWVVEPIYLAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNLLDVDPLEPIQLELDEEEDSAVYTWFYDHKPLVKTKLINGPSYRKWHLSLPIMATLHRLAGQLLSDLIDRNYFYLFDMESFFTAKALNMCIPGGPKFEPLYRDMEKGDEDWNEFNDINKLIIRSPLRTEYRIAFPHLYNNRPRKVKLCVYHTPMIMYIKAEDPDLPAFYYDPLIHPITSSNKERREKKTHDDDDDDEDFVMPEGVEPFLEDTQLYTDTTAAGISLLFANRPFNMRSGRMRRAEDIPLVSEWYKEHCPPSYPVKVRVSYQKLLKCFVLNELHHRPPKAQKKKHLFRSLAATKFFQTTELDWAEAGLQVCKQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDANIQFRLGNVDAFQLADGLQYTFSHVGQLTGMYRYKYRLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRHSKGTAKTVTKQRVESHFDLELRAAVMHDVLDAMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPVPIENMILRYVKSKADWWTNVAHYNRERIRRGATVDKTVCRKNLGRLTRLWLKAEQERQHNYLKDGPYVTPEEAVAIYTTTVHWLESRKFSPIPFPPLSYKHDTKLLILALERLKESYSVAVRLNQLQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSSLIPVYEIEPLEKITDAYLDQYLWYEGDKRHLFPNWIKPADSEPPPLLVYKWCQGINNLQGIWDTSEGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVTAKNNVVLSYKDMSHTNSYGLIRGLQFASFVVQYYGLVLDLLLLGLTRASEIAGPPQMPNEFITYWDTKVETRHPIRLYSRYIDRVHILFRFTHEEARDLIQRYLTEHPDPNNENMVGYQNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENSFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTRDGVWNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVIFYTPKEIGGLGMLSMGHILIPQSDLRYSQQTDVGVTHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAQAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDVIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVLDQELDALEIETVQKETIHPRKSYKMNSSCADVLLFAAHRWPMSKPSLVAESKDVFDQKASNKYWIDVQLRWGDYDSHDIERYTRAKFMDYTTDNMSIYPSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEVAALVRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQSCLKIEKFGDLILKATEPQMVLFNIYDDWLKSISSYTAFSRLILILRALHVNNEKAKMLLKPDKTIVTEPHHIWPSLTDDQWMKVEVALRDLILSDYAKKNNVNTSALTQSEIRDIILGAEITPPSQQRQQIAEIEKQAKEASQLTAVTTRTTNVHGDELIVTTTSPYEQAAFGSKTDWRVRAISATNLYLRVNHIYVNSEDIKETGYTYIMPKNILKKFICIADLRTQISGYLYGISPPDNPQVKEIRCIAMPPQWGTHQQVHLPSALPEHDFLNDLEPLGWMHTQPNELPQLSPQDLTAHARVLENNKQWDGEKCIILTCSFTPGSCSLTAYKLTPSGYEWGRVNKDTGSNPHGYLPTHYEKVQMLLSDRFLGFYMIPDNGPWNYNFMGVKHTVSMKYGIKLGTPREYYHEDHRPTHFLEFSNLEEGETAEGDREDTFT

Target ORF information:

RefSeq Version	XM_024596412.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa pre-mRNA-processing-splicing factor 8A (LOC18096703), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024596412.1

ORF Insert Sequence:

ATGTGGAACG ACACCGGAGA GCAGCATATA GCGCCTCCGG GGACGGCTGG GCCGTCGATC 
CCACCGCCTC CACCGTCGCA GCCTTCTTAC ACCGTACTGG CGCCGTCGCA AACGGTGTCG 
AATCCAGCGG ATGCGGAGGC GAAGCTGGAG GAGAAGGCCA GGAAATGGCA GCAACTTAAC 
ACGAAGAGGT ACAGTGATAA GCGGAAGTTT GGTTTCGTCG AGACGCAAAA GGAGGATATG 
CCGCCGGAAC ACGTGAGGAA GATTATAAGG GACCATGGTG ATATGTCCTC GAAGAAATAT 
CGCCATGATA AACGTGTGTA TCTTGGAGCC CTTAAATTCA TTCCACATGC AGTATACAAG 
CTTCTTGAGA ATATGCCAAT GCCCTGGGAG CAGGTTCGTG ATGTGAAGGT TCTATACCAT 
ATTACAGGAG CGATCACTTT TGTGAATGAA ATTCCATGGG TTGTTGAACC TATTTACTTA 
GCTCAGTGGG GGACAATGTG GATCATGATG CGAAGAGAAA AGAGGGATCG GCGGCATTTC 
AAGAGAATGA GGTTTCCGCC TTTTGATGAT GAGGAACCTC CTTTAGACTA TGCTGATAAT 
TTGTTAGATG TTGATCCTCT AGAGCCTATT CAATTAGAGT TGGACGAAGA AGAAGATTCT 
GCTGTCTATA CTTGGTTTTA TGACCATAAG CCTCTTGTTA AAACGAAGCT TATCAATGGT 
CCTAGTTACA GGAAGTGGCA TCTCTCTCTT CCAATAATGG CGACTCTTCA CCGTCTGGCA 
GGACAGCTTC TTTCTGATCT AATTGACCGC AACTATTTTT ACTTGTTCGA CATGGAGTCA 
TTCTTTACAG CCAAAGCATT GAACATGTGC ATACCTGGCG GGCCTAAGTT TGAACCTTTG 
TATCGTGACA TGGAGAAAGG TGATGAGGAC TGGAACGAAT TCAATGACAT CAACAAACTC 
ATTATTCGGT CACCTTTGCG GACAGAGTAT AGAATTGCAT TTCCTCATCT TTACAATAAT 
AGGCCCCGGA AAGTAAAACT TTGTGTGTAT CACACTCCCA TGATCATGTA CATTAAAGCT 
GAGGATCCTG ATTTACCTGC TTTCTATTAT GATCCTTTGA TACACCCAAT AACCTCCTCT 
AACAAGGAAC GCCGTGAAAA AAAGACTCAT GATGACGATG ATGATGATGA GGATTTTGTT 
ATGCCAGAAG GGGTGGAACC TTTTTTGGAG GATACACAGC TTTATACTGA CACCACAGCT 
GCTGGCATTT CCTTGTTATT TGCTAACCGA CCTTTTAACA TGAGATCTGG ACGGATGCGA 
CGGGCAGAAG ATATACCCCT TGTGTCGGAG TGGTATAAGG AGCACTGTCC TCCATCATAT 
CCTGTCAAAG TTCGAGTTAG CTATCAGAAA TTGTTGAAAT GTTTTGTTTT GAATGAACTG 
CATCATAGAC CGCCAAAAGC TCAGAAGAAG AAGCACTTGT TCCGGTCATT AGCAGCAACT 
AAATTCTTCC AAACCACAGA GCTAGATTGG GCTGAAGCAG GTCTCCAAGT TTGTAAGCAG 
GGGTACAACA TGCTGAATCT ATTGATACAC CGGAAAAATT TAAATTACCT CCACCTTGAT 
TATAATTTTA ACTTGAAGCC TGTGAAGACC CTTACGACAA AGGAACGCAA GAAGTCACGG 
TTTGGGAATG CTTTTCATCT TTGTCGTGAG ATCTTGCGTT TGACAAAGCT TGTGGTTGAT 
GCCAATATAC AATTCAGGTT AGGTAATGTG GATGCTTTCC AATTGGCTGA CGGTCTTCAG 
TATACATTTT CCCATGTTGG TCAGTTGACG GGAATGTACA GATACAAGTA TAGGCTGATG 
CGCCAGATAA GAATGTGCAA AGATCTTAAG CATTTGATAT ATTACCGCTT CAATACTGGC 
CCAGTGGGTA AGGGACCTGG TTGTGGATTT TGGGCACCAA TGTGGAGGGT ATGGCTATTT 
TTCCTACGTG GCATAGTGCC TCTTCTTGAA CGGTGGTTGG GTAATCTTCT TGCAAGACAG 
TTTGAAGGGC GCCATTCAAA AGGAACTGCC AAGACTGTTA CAAAGCAGCG TGTCGAGAGC 
CACTTTGACT TGGAACTCCG TGCCGCTGTT ATGCATGATG TTCTTGATGC CATGCCAGAG 
GGCATTAAGC AAAATAAGGC TAGAACGATA CTGCAGCACC TTAGTGAGGC TTGGCGCTGC 
TGGAAAGCAA ACATTCCATG GAAGGTTCCT GGTTTACCAG TTCCCATTGA AAACATGATA 
CTTCGGTATG TTAAGTCAAA AGCAGACTGG TGGACGAATG TTGCTCACTA TAATCGTGAG 
CGTATCAGAA GGGGTGCGAC TGTTGACAAG ACAGTATGCA GAAAGAATCT TGGAAGACTG 
ACTCGGTTGT GGTTAAAGGC AGAACAGGAA CGTCAACACA ACTATTTGAA GGATGGCCCA 
TATGTCACTC CAGAAGAAGC TGTTGCGATT TACACAACTA CTGTCCATTG GTTGGAGTCC 
AGAAAGTTTT CACCAATTCC ATTCCCTCCC TTGTCATACA AGCATGACAC TAAGCTGCTT 
ATCCTTGCTT TGGAAAGGTT GAAAGAGTCA TACAGTGTGG CTGTGAGATT AAACCAACTG 
CAGAGGGAAG AACTAGGTCT TATTGAACAA GCTTATGACA ACCCCCATGA GGCTTTATCA 
CGGATCAAGC GTCACCTGCT CACTCAGCGT GCTTTCAAAG AAGTGGGCAT AGAGTTCATG 
GATTTGTACA GTTCTCTGAT TCCGGTTTAT GAAATTGAGC CTCTTGAAAA AATAACAGAT 
GCTTATTTGG ACCAATATCT GTGGTATGAA GGTGATAAGC GCCACCTCTT TCCAAACTGG 
ATTAAACCTG CAGATTCCGA GCCACCACCA CTGCTTGTCT ATAAATGGTG TCAAGGCATA 
AACAATTTGC AAGGTATCTG GGACACAAGT GAGGGGCAGT GTGTGGTGAT GCTTCAGACC 
AAGTTTGAGA AGTTCTTTGA AAAGATCGAC TTGACAATGT TAAATAGGCT TCTTCGGTTG 
GTCCTTGACC ACAATATTGC TGATTATGTG ACTGCCAAGA ACAATGTTGT CCTTTCCTAC 
AAGGACATGA GTCACACGAA CTCGTATGGT TTGATACGTG GCCTTCAGTT TGCTTCTTTT 
GTGGTGCAGT ATTATGGACT TGTGCTGGAT CTCCTGCTTC TTGGATTGAC CCGGGCCAGT 
GAGATCGCTG GTCCGCCACA AATGCCAAAT GAATTTATCA CCTACTGGGA TACAAAGGTT 
GAGACACGAC ATCCAATTCG TCTTTATTCC AGGTACATAG ACAGGGTGCA TATATTGTTT 
CGGTTTACTC ATGAAGAGGC CCGAGATCTA ATACAACGAT ATCTCACTGA GCATCCTGAT 
CCCAACAATG AAAATATGGT TGGGTATCAG AACAAGAAAT GCTGGCCTAG AGATGCAAGG 
ATGAGGCTTA TGAAACATGA TGTCAATCTT GGGAGGAGTG TTTTCTGGGA CATGAAGAAT 
CGTCTCCCTC GAAGCATCAC AACTTTAGAG TGGGAGAACA GCTTTGTTTC TGTTTACAGC 
AAGGACAACC CAAACCTGCT TTTCAGCATG TGTGGGTTTG AAGTTCGGAT ACTTCCTAAA 
ATAAGAATGA CTCAAGAAGC ATTCAGCAAT ACAAGAGATG GTGTTTGGAA TTTGCAGAAT 
GAGCAGACCA AGGAACGGAC CGCAGTTGCT TTTCTACGGG TTGATGATGA GCACATGAAA 
GTGTTTGAGA ATCGTGTGAG GCAGATTCTG ATGTCCTCAG GGTCAACTAC TTTCACAAAG 
ATTGTTAACA AATGGAATAC AGCCTTGATT GGATTGATGA CATATTTCCG TGAAGCAACT 
GTGCATACTC AAGAGCTGTT AGATTTGCTT GTAAAATGTG AAAATAAAAT TCAAACCCGT 
ATCAAGATTG GGCTAAACTC AAAAATGCCA AGCAGATTTC CTCCTGTTAT CTTCTATACA 
CCAAAAGAAA TTGGAGGACT TGGCATGTTA TCCATGGGTC ACATATTGAT CCCTCAAAGT 
GATCTTCGAT ATAGTCAGCA AACAGATGTT GGTGTAACCC ATTTCAGGAG CGGAATGAGT 
CATGAGGAGG ACCAGCTGAT TCCAAATCTT TATCGGTACA TTCAGCCATG GGAGAGTGAG 
TTCATTGACT CGCAGCGGGT TTGGGCAGAG TATGCATTGA AGAGGCAGGA AGCGCAGGCG 
CAAAATAGGC GTCTAACACT TGAAGATTTG GAAGATTCAT GGGACAGGGG AATTCCACGC 
ATTAATACTC TTTTTCAGAA AGATCGTCAC ACTTTAGCAT ATGACAAAGG GTGGAGAGTG 
CGCACAGACT TCAAGCAATA CCAAGTTTTA AAACAAAATC CTTTCTGGTG GACGCACCAG 
AGACATGATG GAAAATTGTG GAACTTGAAC AATTATCGAA CTGATGTCAT TCAAGCTCTT 
GGTGGTGTTG AAGGAATTCT TGAGCATACC TTGTTCAAAG GAACATATTT CCCAACTTGG 
GAAGGTCTCT TTTGGGAAAA GGCATCTGGC TTTGAGGAGT CCATGAAGTA TAAGAAGCTT 
ACTAATGCAC AGAGATCTGG TCTGAACCAA ATTCCTAACC GTAGATTTAC ACTTTGGTGG 
TCCCCCACAA TAAACAGGGC AAATGTTTAT GTGGGTTTTC AAGTGCAGTT AGATCTAACC 
GGCATCTTCA TGCACGGGAA GATCCCGACC TTGAAGATTT CGCTGATTCA GATATTCCGT 
GCTCACTTGT GGCAGAAGAT TCATGAGAGT GTTGTTATGG ACCTCTGTCA GGTCTTGGAT 
CAGGAACTGG ATGCTTTGGA AATTGAGACT GTGCAGAAAG AAACAATCCA TCCAAGGAAA 
AGTTATAAAA TGAACAGCTC CTGTGCAGAC GTTCTCCTTT TTGCTGCCCA TAGATGGCCC 
ATGTCAAAGC CTAGTCTTGT GGCTGAGTCA AAAGATGTGT TTGACCAGAA AGCAAGCAAT 
AAATACTGGA TAGATGTACA GCTGAGATGG GGAGACTATG ATTCTCATGA TATTGAGCGC 
TATACTAGGG CCAAATTTAT GGATTACACA ACAGACAACA TGTCTATATA TCCATCTCCA 
ACTGGGGTTA TGATTGGGCT TGATCTAGCG TACAATTTGC ACTCTGCATT TGGCAACTGG 
TTTCCGGGGT CTAAACCACT GCTAGCTCAA GCTATGAACA AAATAATGAA GTCAAACCCA 
GCTTTGTATG TCTTGAGGGA GCGCATAAGA AAAGGTTTGC AGTTGTATTC TTCAGAGCCT 
ACAGAACCAT ATTTATCATC ACAGAATTAT GGGGAGATCT TTAGCAATCA GATAATATGG 
TTTGTTGATG ATACTAATGT CTACCGTGTC ACAATCCACA AGACATTTGA AGGGAATCTC 
ACAACAAAAC CCATAAATGG TGCAATCTTC ATATTCAATC CGAGAACTGG GCAGCTATTT 
TTGAAGGTTA TCCACACTAG TGTATGGGCA GGACAGAAAC GTCTGGGCCA GCTTGCCAAG 
TGGAAAACTG CTGAAGAAGT TGCAGCTCTT GTGCGGTCTC TGCCTGTGGA AGAACAACCA 
AAACAAATTA TTGTGACTCG CAAAGGGATG CTTGATCCAT TGGAGGTTCA CTTGCTTGAT 
TTCCCCAACA TAGTTATCAA GGGAAGTGAG TTGCAGCTCC CATTCCAGTC ATGCTTAAAG 
ATAGAGAAAT TTGGTGATTT GATTTTGAAG GCAACGGAAC CACAAATGGT TTTGTTCAAT 
ATTTATGATG ATTGGCTGAA GAGTATATCA TCTTACACTG CGTTCTCTCG GCTCATTCTG 
ATTCTCCGTG CACTTCATGT TAATAATGAG AAGGCAAAGA TGCTACTCAA GCCTGACAAG 
ACTATCGTTA CTGAGCCACA CCACATCTGG CCTTCACTTA CTGATGATCA GTGGATGAAA 
GTTGAGGTTG CTTTAAGGGA TCTCATACTC TCTGACTATG CCAAGAAGAA CAATGTGAAC 
ACCTCAGCAC TGACACAATC TGAGATACGT GATATTATTC TTGGAGCTGA GATCACTCCT 
CCTTCTCAAC AAAGGCAGCA GATAGCAGAG ATTGAGAAAC AGGCCAAAGA AGCGAGTCAA 
CTGACAGCGG TCACCACAAG GACCACAAAT GTGCATGGTG ATGAACTCAT TGTCACAACC 
ACTAGTCCAT ATGAGCAAGC TGCGTTTGGT TCTAAAACAG ATTGGCGTGT CAGAGCTATA 
TCTGCTACAA ATCTCTATCT TCGAGTCAAT CATATCTATG TGAATTCTGA GGATATCAAG 
GAAACTGGAT ACACATATAT CATGCCGAAG AATATCTTGA AGAAGTTTAT CTGTATAGCT 
GATCTGCGAA CTCAAATTTC TGGGTATTTG TATGGTATAA GCCCCCCGGA CAATCCTCAG 
GTCAAGGAAA TCCGTTGTAT TGCTATGCCC CCACAGTGGG GGACTCATCA ACAGGTTCAT 
CTTCCATCAG CTCTTCCTGA GCATGATTTC CTCAATGATC TGGAGCCTTT GGGATGGATG 
CACACACAAC CAAATGAGCT TCCTCAGTTA TCACCACAGG ATCTCACGGC TCATGCTCGG 
GTTTTGGAGA ACAACAAGCA ATGGGATGGA GAAAAGTGCA TCATCCTGAC ATGTAGTTTC 
ACTCCAGGAT CATGCTCGTT AACTGCATAT AAGCTTACTC CTAGTGGTTA TGAGTGGGGA 
CGTGTCAACA AGGACACGGG AAGCAATCCT CATGGCTACC TGCCAACTCA TTACGAGAAG 
GTTCAGATGC TCCTGAGCGA CCGCTTCCTT GGTTTCTACA TGATTCCTGA TAATGGGCCA 
TGGAATTACA ATTTTATGGG AGTGAAGCAT ACAGTGAGCA TGAAATATGG AATCAAGCTG 
GGGACACCTC GAGAGTATTA CCATGAGGAC CATAGGCCAA CACATTTCCT GGAGTTCAGC 
AACCTGGAGG AGGGTGAGAC CGCTGAAGGC GATAGAGAGG ATACATTCAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC18096703 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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