ANO8 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANO8
Entrez Gene ID	100455974
Full Name	anoctamin 8
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009252952.1	XP_009251227.1	anoctamin-8
XM_024236772.1	XP_024092540.1	anoctamin-8

Pathways from BioSystems
Gene Ontology

The following ANO8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa33665	XM_009252952.1 Latest version!	Pongo abelii anoctamin 8 (ANO8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa173831	XM_024236772.1 Latest version!	Pongo abelii anoctamin 8 (ANO8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANO8 ( XM_009252952.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009251227.1 Pongo abelii anoctamin 8 (ANO8), mRNA.

Price & Availability↑

CloneID	OSa33665
Clone ID Related Accession (Same CDS sequence)	XM_009252952.1
Accession Version	XM_009252952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1411315200000
Organism	Pongo abelii(Sumatran orangutan)
Product	anoctamin-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002889825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCGCCTCCGG CGCCGGGGGC ACGTCCCTGG AGGGCGAGCG TGGCAAGAGG 
CCCCCGCCGG AGGGCGAGCC TGCAGCCCCG GCGTCCGGAG TTCTGGATAA GCTTTTCGGG 
AAGCGGCTCC TGCAGGCGGG TCGCTACCTG GTGTCCCACA AGGCGTGGAT GAAGACGGTG 
CCTACAGAGA ACTGCGACGT GCTGATGACC TTCCCAGACA CGACCGATGA CCACACGCTG 
CTATGGCTGC TGAACCACAT CCGCGTGGGC ATCCCCGAGC TCATCGTGCA AGTCCGCCAC 
CACCGCCACA CGCGTGCCTA CGCCTTCTTT GTCACCGCCA CGTATGAGAG CCTACTCCGA 
GGGGCCGACG AGCTGGGTCT GCGCAAGGCG GTGAAGGCCG AATTTGGCGG GGGCACCCGC 
GGCTTCTCCT GCGAGGAGGA CTTTATCTAT GAGAATGTGG AGAGCGAGCT ACGCTTCTTC 
ACCTCCCAGG AACGCCAGAG CATCATCCGC TTCTGGCTGC AGAATTTGCG TGCCAAGCAG 
GGAGAGGCAC TCCACAACGT GCGCTTCCTG GAGGACCAGC CAATCATCCC CGAGCTGGCA 
GCACGTGGGA TCATCCAGCA GGTGTTCCCT GTCCACGAGC AGCGTATTCT GAACCGCCTC 
ATGAAGTCAT GGGTGCAGGC CGTGTGTGAA AACCAGCCTC TAGATGACAT CTGTGATTAC 
TTTGGTGTGA AAATTGCCAT GTACTTCGCC TGGCTGGGCT TCTACACGTC GGCTATGGTA 
TACCCAGCTG TCTTTGGGTC TGTCCTGTAC ACATTCACAG AGGCTGATCA GACAAGCCGG 
GATGTTTCCT GCGTGGTCTT TGCCTTCTTC AACGTGATCT GGTCAACGCT GTTCCTAGAG 
GAATGGAAGC GGAGGGGGGC TGAGCTGGCA TACAAGTGGG GGACGCTGGA CTCACCTGGG 
GAAGCCGTGG AGGAGCCACG CCCCCAGTTC AGGGGTGTGC GACGTATCAG CCCCATCACG 
CGGGCCGAGG AGTTCTACTA CCCACCCTGG AAGCGGCTGC TCTTCCAGCT GCTTGTGAGC 
CTCCCCCTGT GCCTGGCGTG CCTCGTCTGT GTCTTCTTGC TCATGCTTGG CTGCTTCCAG 
CTGCAGGAGC TGGTGCTGAG CGTGAAGGGG TTGCCCCGTC TCGCCCGATT CCTACCTAAG 
GTCATGCTGG CCCTGCTTGT CAGTGTGAGT GCCGAGGGCT ACAAGAAGCT AGCCATCTGG 
CTCAATGACA TGGAAAATTA CCGGCTGGAG AGCGCCTATG AGAAGCACCT CATCATCAAA 
GTTGTCCTGT TCCAGTTTGT CAACTCGTAC CTGAGCCTCT TCTACATCGG TTTCTACCTC 
AAGGACATGG AGCGCTTGAA AGAGATGCTG GCCACGCTGC TGATCACCCG CCAGTTCCTC 
CAGAACGTGC GCGAGGTCCT GCAGCCGCAC CTGTACCGGC GCCTGGGCCG CGGCGAGCTG 
GGCCTGCGGG CCGTCTGGGA GCTGGCCCGA GCCCTGCTTG GCCTCCTGAG CCTCCGGCGC 
CCTGCGCCCC GCCGCCTCGA ACCCCAGGCG GATGAGGGCG GGGGCGGGGG CGGCGGCAGC 
GGGGCCGGGG GCCGCAGGTG CCTCAGCGGG GGCTGCGGGG CGCCGGAGGA GGAGGAGGAG 
GCGGTGCTGG TGGAGCGGCG GCGGGCGGGG GAAGGCGGGG AGGAGGAGGA CGGGCCTCCA 
GGGGGCAAGG AGGAGGACGA GGACGACGAG GAGGAGGAGG ACGAGGAGGA AGAGGAGGAC 
GAGGAGGAGG GCGAGGAAGG GGGCCTCCTG GACTGCGGGC TCCGGCTGAA GAAGGTCAGC 
TTCGCTGAGC GCGGCGCGGG GCGGCGTCGG CCCGGCCCAA GCCCGGAGGC CCTCCTGGAG 
GAGGGGAGCC CCACTATGGT GGAGAAGGGG CTGGAGCCGG GAGTGTTCAC CCTGGCCGAG 
GAGGACGACG AGGCGGAGGG GGCTCCCGGC AGCCCCGAAC GGGAGCCCCC GGCCATCTTG 
CTCCGCCGGG CCGGGGGCGA GGGCCGAGAC CAGGGGCCCG ACGGGGGCCC GGACCCGGAA 
CCCGGCTCCA ACAGCGATTC GACCCGTAGG CAGAGACGGC AGAACCGGTC GTCTTGGATT 
GACCCGCCGG AGGAGGAACA CTCGCCCCAA CTCACCCAGG CGGAGCTGGA GAGCTGTATG 
AAGAAGTACG AGGACACGTT CCAGGACTAC CAGGAGATGT TCGTGCAGTT CGGCTACGTC 
GTGCTCTTCT CGTCCGCCTT CCCCCTGGCG GCGCTGTGCG CCCTGGTCAA CAACCTCATT 
GAGATCCGCA GCGACGCCTT CAAGCTGTGC ACCGGGCTGC AGCGGCCCTT CGGCCAGCGC 
GTGGAAAGCA TCGGCCAGTG GCAGAAGGTG ATGGAGGCCA TGGGTGTCCT AGCGATTGTG 
GTCAACTGCT ACTTAATCGG CCAGTGCGGG CAGCTGCAGC GCCTCTTCCC CTGGCTGAGC 
CCGGAGGCAG CCATCGTGTC CGTGGTAGTG CTCGAGCACT TCGCTCTGCT CCTCAAGTAC 
CTCATCCACG TGGCCATCCC CGATATCCCG GGCTGGGTGG CCGAGGAAAT GGCCAAGCTG 
GAGTACCAGC GCCGCGAGGC CTTTAAGAGA CACGAGCGCC AGGCCCAGCA TCGCTACCAG 
CAGCAGCAGC GCAGGCGGCG GGAGGAGGAG GAGCGACAGC GCCATGCGGA GAACCTTACG 
CCCTTAACCG CTGCCGAGTG CCCACCCTGT GCCATGGCCG GGCCCCCACC CGCCCCCCAG 
CCCCTGCCGG GGGACGCCAG CTTTTACAGC CTCCCGCCCC CACTGCTGCC GCCCACCTCG 
GATCCCCTCG AGACCCCAGC GCCCTCCCCT AGCCCCAGCC CCAGCCCCCA GGCCGTGTGC 
TGGCCCAGCG GCTGGCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009251227.1
CDS	196..3156
Translation	MAEAASGAGGTSLEGERGKRPPPEGEPAAPASGVLDKLFGKRLLQAGRYLVSHKAWMKTVPTENCDVLMTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFAFFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVFLLMLGCFQLQELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVREVLQPHLYRRLGRGELGLRAVWELARALLGLLSLRRPAPRRLEPQADEGGGGGGGSGAGGRRCLSGGCGAPEEEEEAVLVERRRAGEGGEEEDGPPGGKEEDEDDEEEEDEEEEEDEEEGEEGGLLDCGLRLKKVSFAERGAGRRRPGPSPEALLEEGSPTMVEKGLEPGVFTLAEEDDEAEGAPGSPEREPPAILLRRAGGEGRDQGPDGGPDPEPGSNSDSTRRQRRQNRSSWIDPPEEEHSPQLTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHERQAQHRYQQQQRRRREEEERQRHAENLTPLTAAECPPCAMAGPPPAPQPLPGDASFYSLPPPLLPPTSDPLETPAPSPSPSPSPQAVCWPSGWH

Target ORF information:

RefSeq Version	XM_009252952.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii anoctamin 8 (ANO8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009252952.1

ORF Insert Sequence:

ATGGCCGAGG CCGCCTCCGG CGCCGGGGGC ACGTCCCTGG AGGGCGAGCG TGGCAAGAGG 
CCCCCGCCGG AGGGCGAGCC TGCAGCCCCG GCGTCCGGAG TTCTGGATAA GCTTTTCGGG 
AAGCGGCTCC TGCAGGCGGG TCGCTACCTG GTGTCCCACA AGGCGTGGAT GAAGACGGTG 
CCTACAGAGA ACTGCGACGT GCTGATGACC TTCCCAGACA CGACCGATGA CCACACGCTG 
CTATGGCTGC TGAACCACAT CCGCGTGGGC ATCCCCGAGC TCATCGTGCA AGTCCGCCAC 
CACCGCCACA CGCGTGCCTA CGCCTTCTTT GTCACCGCCA CGTATGAGAG CCTACTCCGA 
GGGGCCGACG AGCTGGGTCT GCGCAAGGCG GTGAAGGCCG AATTTGGCGG GGGCACCCGC 
GGCTTCTCCT GCGAGGAGGA CTTTATCTAT GAGAATGTGG AGAGCGAGCT ACGCTTCTTC 
ACCTCCCAGG AACGCCAGAG CATCATCCGC TTCTGGCTGC AGAATTTGCG TGCCAAGCAG 
GGAGAGGCAC TCCACAACGT GCGCTTCCTG GAGGACCAGC CAATCATCCC CGAGCTGGCA 
GCACGTGGGA TCATCCAGCA GGTGTTCCCT GTCCACGAGC AGCGTATTCT GAACCGCCTC 
ATGAAGTCAT GGGTGCAGGC CGTGTGTGAA AACCAGCCTC TAGATGACAT CTGTGATTAC 
TTTGGTGTGA AAATTGCCAT GTACTTCGCC TGGCTGGGCT TCTACACGTC GGCTATGGTA 
TACCCAGCTG TCTTTGGGTC TGTCCTGTAC ACATTCACAG AGGCTGATCA GACAAGCCGG 
GATGTTTCCT GCGTGGTCTT TGCCTTCTTC AACGTGATCT GGTCAACGCT GTTCCTAGAG 
GAATGGAAGC GGAGGGGGGC TGAGCTGGCA TACAAGTGGG GGACGCTGGA CTCACCTGGG 
GAAGCCGTGG AGGAGCCACG CCCCCAGTTC AGGGGTGTGC GACGTATCAG CCCCATCACG 
CGGGCCGAGG AGTTCTACTA CCCACCCTGG AAGCGGCTGC TCTTCCAGCT GCTTGTGAGC 
CTCCCCCTGT GCCTGGCGTG CCTCGTCTGT GTCTTCTTGC TCATGCTTGG CTGCTTCCAG 
CTGCAGGAGC TGGTGCTGAG CGTGAAGGGG TTGCCCCGTC TCGCCCGATT CCTACCTAAG 
GTCATGCTGG CCCTGCTTGT CAGTGTGAGT GCCGAGGGCT ACAAGAAGCT AGCCATCTGG 
CTCAATGACA TGGAAAATTA CCGGCTGGAG AGCGCCTATG AGAAGCACCT CATCATCAAA 
GTTGTCCTGT TCCAGTTTGT CAACTCGTAC CTGAGCCTCT TCTACATCGG TTTCTACCTC 
AAGGACATGG AGCGCTTGAA AGAGATGCTG GCCACGCTGC TGATCACCCG CCAGTTCCTC 
CAGAACGTGC GCGAGGTCCT GCAGCCGCAC CTGTACCGGC GCCTGGGCCG CGGCGAGCTG 
GGCCTGCGGG CCGTCTGGGA GCTGGCCCGA GCCCTGCTTG GCCTCCTGAG CCTCCGGCGC 
CCTGCGCCCC GCCGCCTCGA ACCCCAGGCG GATGAGGGCG GGGGCGGGGG CGGCGGCAGC 
GGGGCCGGGG GCCGCAGGTG CCTCAGCGGG GGCTGCGGGG CGCCGGAGGA GGAGGAGGAG 
GCGGTGCTGG TGGAGCGGCG GCGGGCGGGG GAAGGCGGGG AGGAGGAGGA CGGGCCTCCA 
GGGGGCAAGG AGGAGGACGA GGACGACGAG GAGGAGGAGG ACGAGGAGGA AGAGGAGGAC 
GAGGAGGAGG GCGAGGAAGG GGGCCTCCTG GACTGCGGGC TCCGGCTGAA GAAGGTCAGC 
TTCGCTGAGC GCGGCGCGGG GCGGCGTCGG CCCGGCCCAA GCCCGGAGGC CCTCCTGGAG 
GAGGGGAGCC CCACTATGGT GGAGAAGGGG CTGGAGCCGG GAGTGTTCAC CCTGGCCGAG 
GAGGACGACG AGGCGGAGGG GGCTCCCGGC AGCCCCGAAC GGGAGCCCCC GGCCATCTTG 
CTCCGCCGGG CCGGGGGCGA GGGCCGAGAC CAGGGGCCCG ACGGGGGCCC GGACCCGGAA 
CCCGGCTCCA ACAGCGATTC GACCCGTAGG CAGAGACGGC AGAACCGGTC GTCTTGGATT 
GACCCGCCGG AGGAGGAACA CTCGCCCCAA CTCACCCAGG CGGAGCTGGA GAGCTGTATG 
AAGAAGTACG AGGACACGTT CCAGGACTAC CAGGAGATGT TCGTGCAGTT CGGCTACGTC 
GTGCTCTTCT CGTCCGCCTT CCCCCTGGCG GCGCTGTGCG CCCTGGTCAA CAACCTCATT 
GAGATCCGCA GCGACGCCTT CAAGCTGTGC ACCGGGCTGC AGCGGCCCTT CGGCCAGCGC 
GTGGAAAGCA TCGGCCAGTG GCAGAAGGTG ATGGAGGCCA TGGGTGTCCT AGCGATTGTG 
GTCAACTGCT ACTTAATCGG CCAGTGCGGG CAGCTGCAGC GCCTCTTCCC CTGGCTGAGC 
CCGGAGGCAG CCATCGTGTC CGTGGTAGTG CTCGAGCACT TCGCTCTGCT CCTCAAGTAC 
CTCATCCACG TGGCCATCCC CGATATCCCG GGCTGGGTGG CCGAGGAAAT GGCCAAGCTG 
GAGTACCAGC GCCGCGAGGC CTTTAAGAGA CACGAGCGCC AGGCCCAGCA TCGCTACCAG 
CAGCAGCAGC GCAGGCGGCG GGAGGAGGAG GAGCGACAGC GCCATGCGGA GAACCTTACG 
CCCTTAACCG CTGCCGAGTG CCCACCCTGT GCCATGGCCG GGCCCCCACC CGCCCCCCAG 
CCCCTGCCGG GGGACGCCAG CTTTTACAGC CTCCCGCCCC CACTGCTGCC GCCCACCTCG 
GATCCCCTCG AGACCCCAGC GCCCTCCCCT AGCCCCAGCC CCAGCCCCCA GGCCGTGTGC 
TGGCCCAGCG GCTGGCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANO8 ( XM_024236772.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024092540.1 Pongo abelii anoctamin 8 (ANO8), mRNA.

Price & Availability↑

CloneID	OSa173831
Clone ID Related Accession (Same CDS sequence)	XM_024236772.1
Accession Version	XM_024236772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519660800000
Organism	Pongo abelii(Sumatran orangutan)
Product	anoctamin-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036922.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCGCCTCCGG CGCCGGGGGC ACGTCCCTGG AGGGCGAGCG TGGCAAGAGG 
CCCCCGCCGG AGGGCGAGCC TGCAGCCCCG GCGTCCGGAG TTCTGGATAA GCTTTTCGGG 
AAGCGGCTCC TGCAGGCGGG TCGCTACCTG GTGTCCCACA AGGCGTGGAT GAAGACGGTG 
CCTACAGAGA ACTGCGACGT GCTGATGACC TTCCCAGACA CGACCGATGA CCACACGCTG 
CTATGGCTGC TGAACCACAT CCGCGTGGGC ATCCCCGAGC TCATCGTGCA AGTCCGCCAC 
CACCGCCACA CGCGTGCCTA CGCCTTCTTT GTCACCGCCA CGTATGAGAG CCTACTCCGA 
GGGGCCGACG AGCTGGGTCT GCGCAAGGCG GTGAAGGCCG AATTTGGCGG GGGCACCCGC 
GGCTTCTCCT GCGAGGAGGA CTTTATCTAT GAGAATGTGG AGAGCGAGCT ACGCTTCTTC 
ACCTCCCAGG AACGCCAGAG CATCATCCGC TTCTGGCTGC AGAATTTGCG TGCCAAGCAG 
GGAGAGGCAC TCCACAACGT GCGCTTCCTG GAGGACCAGC CAATCATCCC CGAGCTGGCA 
GCACGTGGGA TCATCCAGCA GGTGTTCCCT GTCCACGAGC AGCGTATTCT GAACCGCCTC 
ATGAAGTCAT GGGTGCAGGC CGTGTGTGAA AACCAGCCTC TAGATGACAT CTGTGATTAC 
TTTGGTGTGA AAATTGCCAT GTACTTCGCC TGGCTGGGCT TCTACACGTC GGCTATGGTA 
TACCCAGCTG TCTTTGGGTC TGTCCTGTAC ACATTCACAG AGGCTGATCA GACAAGCCGG 
GATGTTTCCT GCGTGGTCTT TGCCTTCTTC AACGTGATCT GGTCAACGCT GTTCCTAGAG 
GAATGGAAGC GGAGGGGGGC TGAGCTGGCA TACAAGTGGG GGACGCTGGA CTCACCTGGG 
GAAGCCGTGG AGGAGCCACG CCCCCAGTTC AGGGGTGTGC GACGTATCAG CCCCATCACG 
CGGGCCGAGG AGTTCTACTA CCCACCCTGG AAGCGGCTGC TCTTCCAGCT GCTTGTGAGC 
CTCCCCCTGT GCCTGGCGTG CCTCGTCTGT GTCTTCTTGC TCATGCTTGG CTGCTTCCAG 
CTGCAGGAGC TGGTGCTGAG CGTGAAGGGG TTGCCCCGTC TCGCCCGATT CCTACCTAAG 
GTCATGCTGG CCCTGCTTGT CAGTGTGAGT GCCGAGGGCT ACAAGAAGCT AGCCATCTGG 
CTCAATGACA TGGAAAATTA CCGGCTGGAG AGCGCCTATG AGAAGCACCT CATCATCAAA 
GTTGTCCTGT TCCAGTTTGT CAACTCGTAC CTGAGCCTCT TCTACATCGG TTTCTACCTC 
AAGGACATGG AGCGCTTGAA AGAGATGCTG GCCACGCTGC TGATCACCCG CCAGTTCCTC 
CAGAACGTGC GCGAGGTCCT GCAGCCGCAC CTGTACCGGC GCCTGGGCCG CGGCGAGCTG 
GGCCTGCGGG CCGTCTGGGA GCTGGCCCGA GCCCTGCTTG GCCTCCTGAG CCTCCGGCGC 
CCTGCGCCCC GCCGCCTCGA ACCCCAGGCG GATGAGGGCG GGGGCGGGGG CGGCGGCAGC 
GGGGCCGGGG GCCGCAGGTG CCTCAGCGGG GGCTGCGGGG CGCCGGAGGA GGAGGAGGAG 
GCGGTGCTGG TGGAGCGGCG GCGGGCGGGG GAAGGCGGGG AGGAGGAGGA CGGGCCTCCA 
GGGGGCAAGG AGGAGGACGA GGACGACGAG GAGGAGGAGG ACGAGGAGGA AGAGGAGGAC 
GAGGAGGAGG GCGAGGAAGG GGGCCTCCTG GACTGCGGGC TCCGGCTGAA GAAGGTCAGC 
TTCGCTGAGC GCGGCGCGGG GCGGCGTCGG CCCGGCCCAA GCCCGGAGGC CCTCCTGGAG 
GAGGGGAGCC CCACTATGGT GGAGAAGGGG CTGGAGCCGG GAGTGTTCAC CCTGGCCGAG 
GAGGACGACG AGGCGGAGGG GGCTCCCGGC AGCCCCGAAC GGGAGCCCCC GGCCATCTTG 
CTCCGCCGGG CCGGGGGCGA GGGCCGAGAC CAGGGGCCCG ACGGGGGCCC GGACCCGGAA 
CCCGGCTCCA ACAGCGATTC GACCCGTAGG CAGAGACGGC AGAACCGGTC GTCTTGGATT 
GACCCGCCGG AGGAGGAACA CTCGCCCCAA CTCACCCAGG CGGAGCTGGA GAGCTGTATG 
AAGAAGTACG AGGACACGTT CCAGGACTAC CAGGAGATGT TCGTGCAGTT CGGCTACGTC 
GTGCTCTTCT CGTCCGCCTT CCCCCTGGCG GCGCTGTGCG CCCTGGTCAA CAACCTCATT 
GAGATCCGCA GCGACGCCTT CAAGCTGTGC ACCGGGCTGC AGCGGCCCTT CGGCCAGCGC 
GTGGAAAGCA TCGGCCAGTG GCAGAAGGTG ATGGAGGCCA TGGGTGTCCT AGCGATTGTG 
GTCAACTGCT ACTTAATCGG CCAGTGCGGG CAGCTGCAGC GCCTCTTCCC CTGGCTGAGC 
CCGGAGGCAG CCATCGTGTC CGTGGTAGTG CTCGAGCACT TCGCTCTGCT CCTCAAGTAC 
CTCATCCACG TGGCCATCCC CGATATCCCG GGCTGGGTGG CCGAGGAAAT GGCCAAGCTG 
GAGTACCAGC GCCGCGAGGC CTTTAAGAGA CACGAGCGCC AGGCCCAGCA TCGCTACCAG 
CAGCAGCAGC GCAGGCGGCG GGAGGAGGAG GAGCGACAGC GCCATGCGGA GCACCATGCC 
CGGCGGGAGC ATGATTCTGG TGGCCGGGAG GAGGCGAGGG CCGAGGGCTC TGGGCTGGAC 
CCTGCCACCT CCTCCGAGAA GGCCTCTGCC AAGGCCAAGG GCAGCACTGC GGGTGGCCAT 
GGGCCTGAAC GGCCCAAGCG CCCAGGGTCC CTGCTGGCAC CCAACAACGT CATGAAGTTG 
AAGCAGATCA TCCCACTGCA GGGCAAATTC CTCTCGTCGG GGGCAACATC CTCACTGGCT 
GCTGCAGGGG CTGGAGCCAC CACCCGGCCT CCCCCTGCCC AGTCACCCAC GGGCAGCGAC 
ACCCGCCTGC CTGCCTTCCT CAGCTTCAAG TTCCTCAAGT CACCAGAGAC CCGGCGGGAC 
TCTGAGCGCA GCCACTCACC GCCCAAAGCC TTCCACGCTG GCAAGCTCTT CCCCTTTGGT 
GGCACCCGGG CTGAGCCTGG GTCCAACGGG GCGGGCGGGC AGGCCCGGCC AGACGGGACC 
CCCAGCAGTG GCAGCAGCCG TGTTCAGAGG AGTGGGCCGG TGGACGAGGC CCTGGCTGAG 
GAGCTGGAAG CCCCCCGGCC CGAAGAGGAA GGCTCAGGGA CAGCGCTGGC CCCCGTGGGC 
GCCCCTGCCC TCCGCACCCG CCGCAGCCGG AGCCCCGCGC CGCCGCCGCC AATGCCGCTG 
CCCCGGCCCC CGACACCGCC CGCCGGCTGC TGGCAGTGGG ACGGGCCCTG GGGCTGCGGG 
GGCGAGGGTG CCGCCCCCCG CCAGGCCCTG GCCGCTGCCG AGTGCCCACC CTGTGCCATG 
GCCGGGCCCC CACCCGCCCC CCAGCCCCTG CCGGGGGACG CCAGCTTTTA CAGCCTCCCG 
CCCCCACTGC TGCCGCCCAC CTCGGATCCC CTCGAGACCC CAGCGCCCTC CCCTAGCCCC 
AGCCCCAGCC CCCAGGCCGT GTGCTGGCCC AGCGGCTGGC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024092540.1
CDS	196..3900
Translation	MAEAASGAGGTSLEGERGKRPPPEGEPAAPASGVLDKLFGKRLLQAGRYLVSHKAWMKTVPTENCDVLMTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFAFFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVFLLMLGCFQLQELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLQNVREVLQPHLYRRLGRGELGLRAVWELARALLGLLSLRRPAPRRLEPQADEGGGGGGGSGAGGRRCLSGGCGAPEEEEEAVLVERRRAGEGGEEEDGPPGGKEEDEDDEEEEDEEEEEDEEEGEEGGLLDCGLRLKKVSFAERGAGRRRPGPSPEALLEEGSPTMVEKGLEPGVFTLAEEDDEAEGAPGSPEREPPAILLRRAGGEGRDQGPDGGPDPEPGSNSDSTRRQRRQNRSSWIDPPEEEHSPQLTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHERQAQHRYQQQQRRRREEEERQRHAEHHARREHDSGGREEARAEGSGLDPATSSEKASAKAKGSTAGGHGPERPKRPGSLLAPNNVMKLKQIIPLQGKFLSSGATSSLAAAGAGATTRPPPAQSPTGSDTRLPAFLSFKFLKSPETRRDSERSHSPPKAFHAGKLFPFGGTRAEPGSNGAGGQARPDGTPSSGSSRVQRSGPVDEALAEELEAPRPEEEGSGTALAPVGAPALRTRRSRSPAPPPPMPLPRPPTPPAGCWQWDGPWGCGGEGAAPRQALAAAECPPCAMAGPPPAPQPLPGDASFYSLPPPLLPPTSDPLETPAPSPSPSPSPQAVCWPSGWH

Target ORF information:

RefSeq Version	XM_024236772.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii anoctamin 8 (ANO8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024236772.1

ORF Insert Sequence:

ATGGCCGAGG CCGCCTCCGG CGCCGGGGGC ACGTCCCTGG AGGGCGAGCG TGGCAAGAGG 
CCCCCGCCGG AGGGCGAGCC TGCAGCCCCG GCGTCCGGAG TTCTGGATAA GCTTTTCGGG 
AAGCGGCTCC TGCAGGCGGG TCGCTACCTG GTGTCCCACA AGGCGTGGAT GAAGACGGTG 
CCTACAGAGA ACTGCGACGT GCTGATGACC TTCCCAGACA CGACCGATGA CCACACGCTG 
CTATGGCTGC TGAACCACAT CCGCGTGGGC ATCCCCGAGC TCATCGTGCA AGTCCGCCAC 
CACCGCCACA CGCGTGCCTA CGCCTTCTTT GTCACCGCCA CGTATGAGAG CCTACTCCGA 
GGGGCCGACG AGCTGGGTCT GCGCAAGGCG GTGAAGGCCG AATTTGGCGG GGGCACCCGC 
GGCTTCTCCT GCGAGGAGGA CTTTATCTAT GAGAATGTGG AGAGCGAGCT ACGCTTCTTC 
ACCTCCCAGG AACGCCAGAG CATCATCCGC TTCTGGCTGC AGAATTTGCG TGCCAAGCAG 
GGAGAGGCAC TCCACAACGT GCGCTTCCTG GAGGACCAGC CAATCATCCC CGAGCTGGCA 
GCACGTGGGA TCATCCAGCA GGTGTTCCCT GTCCACGAGC AGCGTATTCT GAACCGCCTC 
ATGAAGTCAT GGGTGCAGGC CGTGTGTGAA AACCAGCCTC TAGATGACAT CTGTGATTAC 
TTTGGTGTGA AAATTGCCAT GTACTTCGCC TGGCTGGGCT TCTACACGTC GGCTATGGTA 
TACCCAGCTG TCTTTGGGTC TGTCCTGTAC ACATTCACAG AGGCTGATCA GACAAGCCGG 
GATGTTTCCT GCGTGGTCTT TGCCTTCTTC AACGTGATCT GGTCAACGCT GTTCCTAGAG 
GAATGGAAGC GGAGGGGGGC TGAGCTGGCA TACAAGTGGG GGACGCTGGA CTCACCTGGG 
GAAGCCGTGG AGGAGCCACG CCCCCAGTTC AGGGGTGTGC GACGTATCAG CCCCATCACG 
CGGGCCGAGG AGTTCTACTA CCCACCCTGG AAGCGGCTGC TCTTCCAGCT GCTTGTGAGC 
CTCCCCCTGT GCCTGGCGTG CCTCGTCTGT GTCTTCTTGC TCATGCTTGG CTGCTTCCAG 
CTGCAGGAGC TGGTGCTGAG CGTGAAGGGG TTGCCCCGTC TCGCCCGATT CCTACCTAAG 
GTCATGCTGG CCCTGCTTGT CAGTGTGAGT GCCGAGGGCT ACAAGAAGCT AGCCATCTGG 
CTCAATGACA TGGAAAATTA CCGGCTGGAG AGCGCCTATG AGAAGCACCT CATCATCAAA 
GTTGTCCTGT TCCAGTTTGT CAACTCGTAC CTGAGCCTCT TCTACATCGG TTTCTACCTC 
AAGGACATGG AGCGCTTGAA AGAGATGCTG GCCACGCTGC TGATCACCCG CCAGTTCCTC 
CAGAACGTGC GCGAGGTCCT GCAGCCGCAC CTGTACCGGC GCCTGGGCCG CGGCGAGCTG 
GGCCTGCGGG CCGTCTGGGA GCTGGCCCGA GCCCTGCTTG GCCTCCTGAG CCTCCGGCGC 
CCTGCGCCCC GCCGCCTCGA ACCCCAGGCG GATGAGGGCG GGGGCGGGGG CGGCGGCAGC 
GGGGCCGGGG GCCGCAGGTG CCTCAGCGGG GGCTGCGGGG CGCCGGAGGA GGAGGAGGAG 
GCGGTGCTGG TGGAGCGGCG GCGGGCGGGG GAAGGCGGGG AGGAGGAGGA CGGGCCTCCA 
GGGGGCAAGG AGGAGGACGA GGACGACGAG GAGGAGGAGG ACGAGGAGGA AGAGGAGGAC 
GAGGAGGAGG GCGAGGAAGG GGGCCTCCTG GACTGCGGGC TCCGGCTGAA GAAGGTCAGC 
TTCGCTGAGC GCGGCGCGGG GCGGCGTCGG CCCGGCCCAA GCCCGGAGGC CCTCCTGGAG 
GAGGGGAGCC CCACTATGGT GGAGAAGGGG CTGGAGCCGG GAGTGTTCAC CCTGGCCGAG 
GAGGACGACG AGGCGGAGGG GGCTCCCGGC AGCCCCGAAC GGGAGCCCCC GGCCATCTTG 
CTCCGCCGGG CCGGGGGCGA GGGCCGAGAC CAGGGGCCCG ACGGGGGCCC GGACCCGGAA 
CCCGGCTCCA ACAGCGATTC GACCCGTAGG CAGAGACGGC AGAACCGGTC GTCTTGGATT 
GACCCGCCGG AGGAGGAACA CTCGCCCCAA CTCACCCAGG CGGAGCTGGA GAGCTGTATG 
AAGAAGTACG AGGACACGTT CCAGGACTAC CAGGAGATGT TCGTGCAGTT CGGCTACGTC 
GTGCTCTTCT CGTCCGCCTT CCCCCTGGCG GCGCTGTGCG CCCTGGTCAA CAACCTCATT 
GAGATCCGCA GCGACGCCTT CAAGCTGTGC ACCGGGCTGC AGCGGCCCTT CGGCCAGCGC 
GTGGAAAGCA TCGGCCAGTG GCAGAAGGTG ATGGAGGCCA TGGGTGTCCT AGCGATTGTG 
GTCAACTGCT ACTTAATCGG CCAGTGCGGG CAGCTGCAGC GCCTCTTCCC CTGGCTGAGC 
CCGGAGGCAG CCATCGTGTC CGTGGTAGTG CTCGAGCACT TCGCTCTGCT CCTCAAGTAC 
CTCATCCACG TGGCCATCCC CGATATCCCG GGCTGGGTGG CCGAGGAAAT GGCCAAGCTG 
GAGTACCAGC GCCGCGAGGC CTTTAAGAGA CACGAGCGCC AGGCCCAGCA TCGCTACCAG 
CAGCAGCAGC GCAGGCGGCG GGAGGAGGAG GAGCGACAGC GCCATGCGGA GCACCATGCC 
CGGCGGGAGC ATGATTCTGG TGGCCGGGAG GAGGCGAGGG CCGAGGGCTC TGGGCTGGAC 
CCTGCCACCT CCTCCGAGAA GGCCTCTGCC AAGGCCAAGG GCAGCACTGC GGGTGGCCAT 
GGGCCTGAAC GGCCCAAGCG CCCAGGGTCC CTGCTGGCAC CCAACAACGT CATGAAGTTG 
AAGCAGATCA TCCCACTGCA GGGCAAATTC CTCTCGTCGG GGGCAACATC CTCACTGGCT 
GCTGCAGGGG CTGGAGCCAC CACCCGGCCT CCCCCTGCCC AGTCACCCAC GGGCAGCGAC 
ACCCGCCTGC CTGCCTTCCT CAGCTTCAAG TTCCTCAAGT CACCAGAGAC CCGGCGGGAC 
TCTGAGCGCA GCCACTCACC GCCCAAAGCC TTCCACGCTG GCAAGCTCTT CCCCTTTGGT 
GGCACCCGGG CTGAGCCTGG GTCCAACGGG GCGGGCGGGC AGGCCCGGCC AGACGGGACC 
CCCAGCAGTG GCAGCAGCCG TGTTCAGAGG AGTGGGCCGG TGGACGAGGC CCTGGCTGAG 
GAGCTGGAAG CCCCCCGGCC CGAAGAGGAA GGCTCAGGGA CAGCGCTGGC CCCCGTGGGC 
GCCCCTGCCC TCCGCACCCG CCGCAGCCGG AGCCCCGCGC CGCCGCCGCC AATGCCGCTG 
CCCCGGCCCC CGACACCGCC CGCCGGCTGC TGGCAGTGGG ACGGGCCCTG GGGCTGCGGG 
GGCGAGGGTG CCGCCCCCCG CCAGGCCCTG GCCGCTGCCG AGTGCCCACC CTGTGCCATG 
GCCGGGCCCC CACCCGCCCC CCAGCCCCTG CCGGGGGACG CCAGCTTTTA CAGCCTCCCG 
CCCCCACTGC TGCCGCCCAC CTCGGATCCC CTCGAGACCC CAGCGCCCTC CCCTAGCCCC 
AGCCCCAGCC CCCAGGCCGT GTGCTGGCCC AGCGGCTGGC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ANO8 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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