EMILIN2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMILIN2
Entrez Gene ID	100453283
Full Name	elastin microfibril interfacer 2
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024235891.1	XP_024091659.1	EMILIN-2
XM_009252273.1	XP_009250548.1	LOW QUALITY PROTEIN: EMILIN-2

Pathways from BioSystems
Gene Ontology

The following EMILIN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMILIN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa173112	XM_024235891.1 Latest version!	Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa32741	XM_009252273.1 Latest version!	Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMILIN2 ( XM_024235891.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024091659.1 Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Price & Availability↑

CloneID	OSa173112
Clone ID Related Accession (Same CDS sequence)	XM_024235891.1
Accession Version	XM_024235891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519660800000
Organism	Pongo abelii(Sumatran orangutan)
Product	EMILIN-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGC CGAGACGACC CTGGCCCCGC GCGCCCTGGC GCTGGGCACT GGCGCTGCTG 
GCCCTGGGCG GCGCGGGACT GTGCCACGCC GGCTCGCAGC CCGGGTATCC CGCGCGGCCC 
AGCGCCAGGA ACAAGAACTG GTGCGCCTAC ATCGTGAACA AGAATGTGAG CTGCTCTGTG 
CTGGAGGGAA GTGAGAGTTT TATTCAGGCT CAGTACAACT GTGCCTGGAA CCAGATGCCC 
TGTCCGTCCG CGCTGGTGTA TCGAGTGAAC TTCAGACCTA GATATGTCAC TAGGTATAAG 
ACAGTGACAC AGTTGGAATG GAGGTGCTGT CCTGGCTTTA GAGGGGGAGA TTGCCAAGAA 
GGTCCCAAAG ACCCCGTGAA GACCCTCCAC CCCACGCCGG CTCGGCCTCG AAACAGCTTG 
AAGAAAGCCA CAGATAATGA ACCCAGCCAA TTCTCAGAGC CCAAGAAGAC TTTGTCCCCA 
ACTGGTACAG CACAACCAAG CTGGGGGGTA GATCCAAAAG AGGGGCCTCA GGAACTTCAG 
GAAAAGAAGA TACAGGTGCT AGAGGAGAAG GTTCTTCGAC TCACAAGGAC AGTTCTTGAC 
CTCCAGTCTT CCCTTGCTGG AGTGAGTGAA AATCTCAAAC ATGCCACTCA GGATGATGCC 
AGTAAAACAC GGGCACCAGG GCTCAGCAGC CAGCACCCCA TGCCTGACAC CACTGTTAGT 
GGAGACACAG AAATGGGCCG GAGTCCTGGT GTCTTCAACA CTAAGGAATC TGGCGTGAAG 
GACATCAAGT CTGAATTGGC TGAAGTCAAA GATACTCTAA AGAACAAAAG TGACAAGCTG 
GAAGAGCTGG ATGGAAAAGT GAAGGGCTAC GAAGGGCAGC TCAGACAGCT CCAGGAAGCA 
GCCCAGGGCC CGACGGTGAC CATGACAACC AACGAACTCT ACCAAGCCTA TGTGGACAGT 
AAGATCGACG CCCTGAGAGA GGAGCTCATG GAGGGCATGG ACAGAAAGCT GGCTGACCTG 
AAAAACTCAT GTGAGTACAA GCTCACTGGC CTCCCGCAGC AGTGTGATGA CTATGGGAGC 
AGCTACCTGG GAGTGATAGA GCTCATAGGG GAGAAGGAAA CAAGCCTGAG AAAAGAAATA 
AATAACCTCC GAGCCCGGCT ACAGGAGCCT TCAGCCCAGG CAAATTGCTG CGACAGTGAA 
AAGAATGGTG ACATTGGTCA ACAGATCAAA ACATTGGACC AGAAAATTGA GAGAGTTGCT 
GAAGCCACCA GAATGCTGAA TGGAAGACTG GACAATGAGT TTGACCGCCT TATAGTTCCA 
GAGCCAGATG TGGATTTTGA TGCAAAATGG AATGAACTCG ATGCAAGGAT CAACGTGACG 
GAGAAGAACG CTGAAGAACA TTGCTTTTAC ATTGAGGAAA CCCTTCGGGG GACCATTAAT 
GGAGAGGTGG GTGACTTGAA GCAGCTTGTT GATCAGAAAA TACAGTCTCT GGAAGACCGT 
CTGGGGAGCG TTCTCCTACA GATGGCCAAC AACACTGGTG CAGAGCTCAG TCCCCCAGGG 
GCAGCAGCCC TGCCAGGAGT GTCAGGGTCA GGAGGTGAAC GGGTCATGAT GGAATTAAAC 
CACCTGAAGG ACAAAGTTCA AGTTGTTGAA GACATTTGCC TGCAGAACAT CCAGGGAAAG 
CCTCATGGGA TGGAAGGTGC CTTGCCAAAC AGGGAAGACC GCACCGTACG CGACAGCCTG 
CACCTTTTGA AATCTCTCAA CGACACGATG CACAGGAAGT TTCGAGAAAC TGAACGAACC 
ATCCAGAAAC TTCAGCAGGA TTTTAGTTTT CTTTATTCTC AATTAAACCA CACAGAAAAT 
GATGTGACTC GTCTTCAGAA GGAAATGAGC AATTGTAGAG CAGGTGAAAA TGCTGGCATG 
GGTGGGTTCA CTAAGGTGGA TGAGCAAGAA AAGACAGTGG ACACCCTGCC ATCCCCCCAG 
CACCCCACGG CTCATTGCTG CAGTCAGCTG GAGGAGAGGT GGCAGAGGTT GCAGAGCCAG 
GTCATCTCGG AGCTGGATGC TTGTAAGGAA AGCACGCAGG GGGTCCAGAG GGAGGTCTCC 
ATGGTGGAGG GCAGGGTGTC TCATATGGAG AAAACTTGCA GCAAGCTGGA CTCTATCTCA 
GGAAATCTTC AGAGGATCAA GGAGGGGCTC AACAAGCATG TCAGCAGCCT GTGGAACTGT 
GTCAGGCAGA TGAACGGAAC GCTCAGGTCG CATTCCAGAG ACATTTCTGG CCTGAAGAAT 
TCAGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTA CTGATTTGCA AGATCTGGTC 
AAATTTCAAC CATCAGCAAA GGAGCCCTCG GAGGCGCCCT CGCCCCCGCC GCCCGCAGAG 
GCTCCCAAAG AGCCGCCGCA GCCCGAGCCC ACCCCGCCGA GGCCCAGCGG CCCCGCAACC 
CCAGACGACC CTGGGCGACG GCCGTCCGCG GGCCATCGGC CCGTCCTGCC TCATTGGCCT 
GTCCTGCCCC AGCGGCCCCT GCTGCCGCGG CCCCCCGAGG AGAGGCCGCC CCCGCCCGCC 
TGGCCCGGCC GGAATGCGCT GCCCCAGCTG CCAGGCTCCA CTGGGGTCAT CATGGAGACG 
GGCCAGGCCG GGCCTCCCGC AGGCGCAGGC GTGTCTGGGC GGGGTCTGCC GCGGGGCGTG 
GATGGCCAGA CCGGGAGCGG GACCGTCCCC AGCGCAGAAG GCTTTGCGGG CGCACCAGGA 
TACCCGAAGT CACCTCCTGT AGCTTCCCCA GGAGCTCCGG TGCCTTCTCT GGTGTCTTTT 
TCTGCGGGGC TCACCCAGAA GCCTTTCCCC AGCGATGGGG GAGTTGTCCT CTTTAACAAA 
GTGCTGGTGA ACGACGGGGA CGTTTACAAT CCCAGCACGG GGGTCTTCAC GGCTCCTTAT 
GATGGGCGTT ACCTGATCAC GGCGACCCTC ACCCCCGAGA GAGACGCCTA CGTGGAAGCA 
GTGCTGTCAG TCTCCAACGC CAGCGTGGCC CAGCTGCATA CCGCTGGGTA CAGGAGAGAG 
TTCCTGGAAT ACCACCGCCC TCCAGGGGCT TTGCACACCT GCGGGGGCCC AGGGGCATTC 
CACCTCATCG TGCACCTGAA GGCGGGAGAT GCAGTCAACA TCGTGGTGAC TGGGGGCAAG 
CTGGCTCACA CAGACTTTGA CGAAATGTAC TCCACATTTA GTGGGGTTTT CTTATATCCT 
TTCCTTTCCC ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024091659.1
CDS	150..3407
Translation	MWQPRRPWPRAPWRWALALLALGGAGLCHAGSQPGYPARPSARNKNWCAYIVNKNVSCSVLEGSESFIQAQYNCAWNQMPCPSALVYRVNFRPRYVTRYKTVTQLEWRCCPGFRGGDCQEGPKDPVKTLHPTPARPRNSLKKATDNEPSQFSEPKKTLSPTGTAQPSWGVDPKEGPQELQEKKIQVLEEKVLRLTRTVLDLQSSLAGVSENLKHATQDDASKTRAPGLSSQHPMPDTTVSGDTEMGRSPGVFNTKESGVKDIKSELAEVKDTLKNKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLTGLPQQCDDYGSSYLGVIELIGEKETSLRKEINNLRARLQEPSAQANCCDSEKNGDIGQQIKTLDQKIERVAEATRMLNGRLDNEFDRLIVPEPDVDFDAKWNELDARINVTEKNAEEHCFYIEETLRGTINGEVGDLKQLVDQKIQSLEDRLGSVLLQMANNTGAELSPPGAAALPGVSGSGGERVMMELNHLKDKVQVVEDICLQNIQGKPHGMEGALPNREDRTVRDSLHLLKSLNDTMHRKFRETERTIQKLQQDFSFLYSQLNHTENDVTRLQKEMSNCRAGENAGMGGFTKVDEQEKTVDTLPSPQHPTAHCCSQLEERWQRLQSQVISELDACKESTQGVQREVSMVEGRVSHMEKTCSKLDSISGNLQRIKEGLNKHVSSLWNCVRQMNGTLRSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSAKEPSEAPSPPPPAEAPKEPPQPEPTPPRPSGPATPDDPGRRPSAGHRPVLPHWPVLPQRPLLPRPPEERPPPPAWPGRNALPQLPGSTGVIMETGQAGPPAGAGVSGRGLPRGVDGQTGSGTVPSAEGFAGAPGYPKSPPVASPGAPVPSLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGALHTCGGPGAFHLIVHLKAGDAVNIVVTGGKLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_024235891.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024235891.1

ORF Insert Sequence:

ATGTGGCAGC CGAGACGACC CTGGCCCCGC GCGCCCTGGC GCTGGGCACT GGCGCTGCTG 
GCCCTGGGCG GCGCGGGACT GTGCCACGCC GGCTCGCAGC CCGGGTATCC CGCGCGGCCC 
AGCGCCAGGA ACAAGAACTG GTGCGCCTAC ATCGTGAACA AGAATGTGAG CTGCTCTGTG 
CTGGAGGGAA GTGAGAGTTT TATTCAGGCT CAGTACAACT GTGCCTGGAA CCAGATGCCC 
TGTCCGTCCG CGCTGGTGTA TCGAGTGAAC TTCAGACCTA GATATGTCAC TAGGTATAAG 
ACAGTGACAC AGTTGGAATG GAGGTGCTGT CCTGGCTTTA GAGGGGGAGA TTGCCAAGAA 
GGTCCCAAAG ACCCCGTGAA GACCCTCCAC CCCACGCCGG CTCGGCCTCG AAACAGCTTG 
AAGAAAGCCA CAGATAATGA ACCCAGCCAA TTCTCAGAGC CCAAGAAGAC TTTGTCCCCA 
ACTGGTACAG CACAACCAAG CTGGGGGGTA GATCCAAAAG AGGGGCCTCA GGAACTTCAG 
GAAAAGAAGA TACAGGTGCT AGAGGAGAAG GTTCTTCGAC TCACAAGGAC AGTTCTTGAC 
CTCCAGTCTT CCCTTGCTGG AGTGAGTGAA AATCTCAAAC ATGCCACTCA GGATGATGCC 
AGTAAAACAC GGGCACCAGG GCTCAGCAGC CAGCACCCCA TGCCTGACAC CACTGTTAGT 
GGAGACACAG AAATGGGCCG GAGTCCTGGT GTCTTCAACA CTAAGGAATC TGGCGTGAAG 
GACATCAAGT CTGAATTGGC TGAAGTCAAA GATACTCTAA AGAACAAAAG TGACAAGCTG 
GAAGAGCTGG ATGGAAAAGT GAAGGGCTAC GAAGGGCAGC TCAGACAGCT CCAGGAAGCA 
GCCCAGGGCC CGACGGTGAC CATGACAACC AACGAACTCT ACCAAGCCTA TGTGGACAGT 
AAGATCGACG CCCTGAGAGA GGAGCTCATG GAGGGCATGG ACAGAAAGCT GGCTGACCTG 
AAAAACTCAT GTGAGTACAA GCTCACTGGC CTCCCGCAGC AGTGTGATGA CTATGGGAGC 
AGCTACCTGG GAGTGATAGA GCTCATAGGG GAGAAGGAAA CAAGCCTGAG AAAAGAAATA 
AATAACCTCC GAGCCCGGCT ACAGGAGCCT TCAGCCCAGG CAAATTGCTG CGACAGTGAA 
AAGAATGGTG ACATTGGTCA ACAGATCAAA ACATTGGACC AGAAAATTGA GAGAGTTGCT 
GAAGCCACCA GAATGCTGAA TGGAAGACTG GACAATGAGT TTGACCGCCT TATAGTTCCA 
GAGCCAGATG TGGATTTTGA TGCAAAATGG AATGAACTCG ATGCAAGGAT CAACGTGACG 
GAGAAGAACG CTGAAGAACA TTGCTTTTAC ATTGAGGAAA CCCTTCGGGG GACCATTAAT 
GGAGAGGTGG GTGACTTGAA GCAGCTTGTT GATCAGAAAA TACAGTCTCT GGAAGACCGT 
CTGGGGAGCG TTCTCCTACA GATGGCCAAC AACACTGGTG CAGAGCTCAG TCCCCCAGGG 
GCAGCAGCCC TGCCAGGAGT GTCAGGGTCA GGAGGTGAAC GGGTCATGAT GGAATTAAAC 
CACCTGAAGG ACAAAGTTCA AGTTGTTGAA GACATTTGCC TGCAGAACAT CCAGGGAAAG 
CCTCATGGGA TGGAAGGTGC CTTGCCAAAC AGGGAAGACC GCACCGTACG CGACAGCCTG 
CACCTTTTGA AATCTCTCAA CGACACGATG CACAGGAAGT TTCGAGAAAC TGAACGAACC 
ATCCAGAAAC TTCAGCAGGA TTTTAGTTTT CTTTATTCTC AATTAAACCA CACAGAAAAT 
GATGTGACTC GTCTTCAGAA GGAAATGAGC AATTGTAGAG CAGGTGAAAA TGCTGGCATG 
GGTGGGTTCA CTAAGGTGGA TGAGCAAGAA AAGACAGTGG ACACCCTGCC ATCCCCCCAG 
CACCCCACGG CTCATTGCTG CAGTCAGCTG GAGGAGAGGT GGCAGAGGTT GCAGAGCCAG 
GTCATCTCGG AGCTGGATGC TTGTAAGGAA AGCACGCAGG GGGTCCAGAG GGAGGTCTCC 
ATGGTGGAGG GCAGGGTGTC TCATATGGAG AAAACTTGCA GCAAGCTGGA CTCTATCTCA 
GGAAATCTTC AGAGGATCAA GGAGGGGCTC AACAAGCATG TCAGCAGCCT GTGGAACTGT 
GTCAGGCAGA TGAACGGAAC GCTCAGGTCG CATTCCAGAG ACATTTCTGG CCTGAAGAAT 
TCAGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTA CTGATTTGCA AGATCTGGTC 
AAATTTCAAC CATCAGCAAA GGAGCCCTCG GAGGCGCCCT CGCCCCCGCC GCCCGCAGAG 
GCTCCCAAAG AGCCGCCGCA GCCCGAGCCC ACCCCGCCGA GGCCCAGCGG CCCCGCAACC 
CCAGACGACC CTGGGCGACG GCCGTCCGCG GGCCATCGGC CCGTCCTGCC TCATTGGCCT 
GTCCTGCCCC AGCGGCCCCT GCTGCCGCGG CCCCCCGAGG AGAGGCCGCC CCCGCCCGCC 
TGGCCCGGCC GGAATGCGCT GCCCCAGCTG CCAGGCTCCA CTGGGGTCAT CATGGAGACG 
GGCCAGGCCG GGCCTCCCGC AGGCGCAGGC GTGTCTGGGC GGGGTCTGCC GCGGGGCGTG 
GATGGCCAGA CCGGGAGCGG GACCGTCCCC AGCGCAGAAG GCTTTGCGGG CGCACCAGGA 
TACCCGAAGT CACCTCCTGT AGCTTCCCCA GGAGCTCCGG TGCCTTCTCT GGTGTCTTTT 
TCTGCGGGGC TCACCCAGAA GCCTTTCCCC AGCGATGGGG GAGTTGTCCT CTTTAACAAA 
GTGCTGGTGA ACGACGGGGA CGTTTACAAT CCCAGCACGG GGGTCTTCAC GGCTCCTTAT 
GATGGGCGTT ACCTGATCAC GGCGACCCTC ACCCCCGAGA GAGACGCCTA CGTGGAAGCA 
GTGCTGTCAG TCTCCAACGC CAGCGTGGCC CAGCTGCATA CCGCTGGGTA CAGGAGAGAG 
TTCCTGGAAT ACCACCGCCC TCCAGGGGCT TTGCACACCT GCGGGGGCCC AGGGGCATTC 
CACCTCATCG TGCACCTGAA GGCGGGAGAT GCAGTCAACA TCGTGGTGAC TGGGGGCAAG 
CTGGCTCACA CAGACTTTGA CGAAATGTAC TCCACATTTA GTGGGGTTTT CTTATATCCT 
TTCCTTTCCC ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_009252273.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009250548.1 Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Price & Availability↑

CloneID	OSa32741
Clone ID Related Accession (Same CDS sequence)	XM_009252273.1
Accession Version	XM_009252273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1411315200000
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: EMILIN-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002889108.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 16% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGGCAGC CGAGACGACC CTGGCCCCGC GCGCCCTGGC GCTGGGCACT GGCGCTGCTG 
GCCCTGGGCG GCGCGGGACT GTGCCACGCC GGCTCGCAGC CCGGGTATCC CGCGCGGCCC 
AGCGCCAGGA ACAAGAACTG GTGCGCCTAC ATCGTGAACA AGAATGTGAG CTGCTCTGTG 
CTGGAGGGAA GTGAGAGTTT TATTCAGGCT CAGTACAACT GTGCCTGGAA CCAGATGCCC 
TGTCCGTCCG CGCTGGTGTA TCGAGTGAAC TTCAGACCTA GATATGTCAC TAGGTATAAG 
ACAGTGACAC AGTTGGAATG GAGGTGCTGT CCTGGCTTTA GAGGGGGAGA TTGCCAAGAA 
GGTCCCAAAG ACCCCGTGAA GACCCTCCAC CCCACGCCGG CTCGGCCTCG AAACAGCTTG 
AAGAAAGCCA CAGATAATGA ACCCAGCCAA TTCTCAGAGC CCAAGAAGAC TTTGTCCCCA 
ACTGGTACAG CACAACCAAG CTGGGGGGTA GATCCAAAAG AGGGGCCTCA GGAACTTCAG 
GAAAAGAAGA TACAGGTGCT AGAGGAGAAG GTTCTTCGAC TCACAAGGAC AGTTCTTGAC 
CTCCAGTCTT CCCTTGCTGG AGTGAGTGAA AATCTCAAAC ATGCCACTCA GGATGATGCC 
AGTAAAACAC GGGCACCAGG GCTCAGCAGC CAGCACCCCA TGCCTGACAC CACTGTTAGT 
GGAGACACAG AAACGGGCCG GAGTCCTGGT GTCTTCAACA CTAAGGAATC TGGCGTGAAG 
GACATCAAGT CTGAATTGGC TGAAGTCAAA GATACTCTAA AGAACAAAAG TGACAAGCTG 
GAAGAGCTGG ATGGAAAAGT GAAGGGCTAC GAAGGGCAGC TCAGACAGCT CCAGGAAGCA 
GCCCAGGGCC CGACGGTGAC CATGACAACC AACGAACTCT ACCAAGCCTA TGTGGACAGT 
AAGATCGACG CCCTGAGAGA GGAGCTCATG GAGGGCATGG ACAGAAAGCT GGCTGACCTG 
AAAAACTCAT GTGAGTACAA GCTCACTGGC CTCCCGCAGC AGTGTGATGA CTATGGGAGC 
AGCTACCTGG GAGTGATAGA GCTCATAGGG GAGAAGGAAA CAAGCCTGAG AAAAGAAATA 
AATAACCTCC GAGCCCGGCT ACAGGAGCCT TCAGCCCAGG CAAATTGCTG CGACAGTGAA 
AAGAATGGTG ACATTGGTCA ACAGATCAAA ACATTGGACC AGAAAATTGA GAGAGTTGCT 
GAAGCCACCA GAATGCTGAA TGGAAGACTG GACAATGAGT TTGACCGCCT TATAGTTCCA 
GAGCCAGATG TGGATTTTGA TGCAAAATGG AATGAACTCG ATGCAAGGAT CAACGTGACG 
GAGAAGAACG CTGAAGAACA TTGCTTTTAC ATTGAGGAAA CCCTTCGGGG GACCATTAAT 
GGAGAGGTGG GTGACTTGAA GCAGCTTGTT GATCAGAAAA TACAGTCTCT GGAAGACCGT 
CTGGGGAGCG TTCTCCTACA GATGGCCAAC AACACTGGTG CAGAGCTCAG TCCCCCAGGG 
GCAGCAGCCC TGCCAGGAGT GTCAGGGTCA GGAGGTGAAC GGGTCATGAT GGAATTAAAC 
CACCTGAAGG ACAAAGTTCA AGTTGTTGAA GACATTTGCC TGCAGAACAT CCAGGGAAAG 
CCTCATGGGA TGGAAGGTGC CTTGCCAAAC AGGGAAGACC GCACCGTACG CGACAGCCTG 
CACCTTTTGA AATCTCTCAA CGACACGATG CACAGGAAGT TTCGAGAAAC TGAACGAACC 
ATCCAGAAAC TTCAGCAGGA TTTTAGTTTT CTTTATTCTC AATTAAACCA CACAGAAAAT 
GATGTGACTC GTCTTCAGAA GGAAATGAGC AATTGTAGAG CAGGTGAAAA TGCTGGCATG 
GGTGGGTTCA CTAAGGTGGA TGAGCAAGAA AGGACAGTGG ACACCCTGCC ATCCCCCCAG 
CACCCCACGG CTCATTGCTG CAGTCAGCTG GAGGAGAGGT GGCAGAGGTT GCAGAGCCAG 
GTCATCTCGG AGCTGGATGC TTGTAAGGAA AGCACGCAGG GGGTCCAGAG GGAGGTCTCC 
ATGGTGGAGG GCAGGGTGTC TCATATGGAG AAAACTTGCA GCAAGCTGGA CTCTATCTCA 
GGAAATCTTC AGAGGATCAA GGAGGGGCTC AACAAGCATG TCAGCAGCCT GTGGAACTGT 
GTCAGGCAGA TGAACGGAAC GCTCAGGTCG CATTCCAGAG ACATTTCTGG CCTGAAGAAT 
TCAGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTA CTGATTTGCA AGATCTGGTC 
AAATTTCAAC CATCAGCAAC CCGAGGGATA CTTTTACATG CCTACTTGTC AGCCACCTTG 
GTGGCCTGCC CTCCTGTCCC GCGGGGGAGA CCAACAACTG TTTCTTCGAA AAAGCCCCTT 
TATCTAAGCT CACCCCGGGC CCATTCAGCA CCACAAGCGA CAGTTTCTCT GAATTTTCTG 
ATGAGTCCAG CATTTCTCAT GCTTCAGTCC GTGATGACTG ATGGGGGCGG GGGGGATGGC 
GTTTCTTTGC GAGTGATTCC TCAGCCAGAC CCGGGTATTT TTAGGCGTCC TGTGGACGGT 
CGGGAGGGGC TTCCTGGAGG GCTAGTTCCA GGAACGAATT CAAAGGAGGA AAGGGCGGCA 
GCTTCCGGCT GCTTCCCAAG AGGGCCGGGA AATGTAAATC AGGAGGTTCC TCCGTCGGAA 
GTCCCTTCGG CTGGCGACGT GAGCGCTTTG GCTGTGGCCC CCGTGGAGCC TGAGCCCTCC 
GCACGGCCTC AGCGCAGCCT TGGGGCCTCC CGGCAGGGTC CCTCGGCAGC CCGCGGGAGA 
GGCCGTGGGG CCCTGGGAGG AGCGTGGTTC AGAGTCTGTG AGGGGGCAGG GACTCAAGCA 
GCAGCAGGAG CGTCTAGGCG GGGTNAGGAG CGGGGCGTGG ATGGCCAGAC GAAGAAATAT 
AAAATCCCCA GCGCAGAAGG CTTTGCGGGC GCACCAGGAT ACCCGAAGTC ACCTCCTGTA 
GCTTCCCCAG GAGCTCCGGT GCCTTCTCTG GTGTCTTTTT CTGCGGGGCT CACCCAGAAG 
CCTTTCCCCA GCGATGGGGG AGTTGTCCTC TTTAACAAAG TGCTGGTGAA CGACGGGGAC 
GTTTACAATC CCAGCACGGG GGTCTTCACG GCTCCTTATG ATGGGCGTTA CCTGATCACG 
GCGACCCTCA CCCCCGAGAG AGACGCCTAC GTGGAAGCAG TGCTGTCAGT CTCCAACGCC 
AGCGTGGCCC AGCTGCATAC CGCTGGGTAC AGGAGAGAGT TCCTGGAATA CCACCGCCCT 
CCAGGGGCTT TGCACACCTG CGGGGGCCCA GGGGCATTCC ACCTCATCGT GCACCTGAAG 
GCGGGAGATG CAGTCAACAT CGTGGTGACT GGGGGCAAGC TGGCTCACAC AGACTTTGAC 
GAAATGTACT CCACATTTAG TGGGGTTTTC TTATATCCTT TCCTTTCCCA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009250548.1
CDS	17..3553
Translation	MWQPRRPWPRAPWRWALALLALGGAGLCHAGSQPGYPARPSARNKNWCAYIVNKNVSCSVLEGSESFIQAQYNCAWNQMPCPSALVYRVNFRPRYVTRYKTVTQLEWRCCPGFRGGDCQEGPKDPVKTLHPTPARPRNSLKKATDNEPSQFSEPKKTLSPTGTAQPSWGVDPKEGPQELQEKKIQVLEEKVLRLTRTVLDLQSSLAGVSENLKHATQDDASKTRAPGLSSQHPMPDTTVSGDTETGRSPGVFNTKESGVKDIKSELAEVKDTLKNKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLTGLPQQCDDYGSSYLGVIELIGEKETSLRKEINNLRARLQEPSAQANCCDSEKNGDIGQQIKTLDQKIERVAEATRMLNGRLDNEFDRLIVPEPDVDFDAKWNELDARINVTEKNAEEHCFYIEETLRGTINGEVGDLKQLVDQKIQSLEDRLGSVLLQMANNTGAELSPPGAAALPGVSGSGGERVMMELNHLKDKVQVVEDICLQNIQGKPHGMEGALPNREDRTVRDSLHLLKSLNDTMHRKFRETERTIQKLQQDFSFLYSQLNHTENDVTRLQKEMSNCRAGENAGMGGFTKVDEQERTVDTLPSPQHPTAHCCSQLEERWQRLQSQVISELDACKESTQGVQREVSMVEGRVSHMEKTCSKLDSISGNLQRIKEGLNKHVSSLWNCVRQMNGTLRSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATRGILLHAYLSATLVACPPVPRGRPTTVSSKKPLYLSSPRAHSAPQATVSLNFLMSPAFLMLQSVMTDGGGGDGVSLRVIPQPDPGIFRRPVDGREGLPGGLVPGTNSKEERAAASGCFPRGPGNVNQEVPPSEVPSAGDVSALAVAPVEPEPSARPQRSLGASRQGPSAARGRGRGALGGAWFRVCEGAGTQAAAGASRRGnullERGVDGQTKKYKIPSAEGFAGAPGYPKSPPVASPGAPVPSLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGALHTCGGPGAFHLIVHLKAGDAVNIVVTGGKLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_009252273.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009252273.1

ORF Insert Sequence:

ATGTGGCAGC CGAGACGACC CTGGCCCCGC GCGCCCTGGC GCTGGGCACT GGCGCTGCTG 
GCCCTGGGCG GCGCGGGACT GTGCCACGCC GGCTCGCAGC CCGGGTATCC CGCGCGGCCC 
AGCGCCAGGA ACAAGAACTG GTGCGCCTAC ATCGTGAACA AGAATGTGAG CTGCTCTGTG 
CTGGAGGGAA GTGAGAGTTT TATTCAGGCT CAGTACAACT GTGCCTGGAA CCAGATGCCC 
TGTCCGTCCG CGCTGGTGTA TCGAGTGAAC TTCAGACCTA GATATGTCAC TAGGTATAAG 
ACAGTGACAC AGTTGGAATG GAGGTGCTGT CCTGGCTTTA GAGGGGGAGA TTGCCAAGAA 
GGTCCCAAAG ACCCCGTGAA GACCCTCCAC CCCACGCCGG CTCGGCCTCG AAACAGCTTG 
AAGAAAGCCA CAGATAATGA ACCCAGCCAA TTCTCAGAGC CCAAGAAGAC TTTGTCCCCA 
ACTGGTACAG CACAACCAAG CTGGGGGGTA GATCCAAAAG AGGGGCCTCA GGAACTTCAG 
GAAAAGAAGA TACAGGTGCT AGAGGAGAAG GTTCTTCGAC TCACAAGGAC AGTTCTTGAC 
CTCCAGTCTT CCCTTGCTGG AGTGAGTGAA AATCTCAAAC ATGCCACTCA GGATGATGCC 
AGTAAAACAC GGGCACCAGG GCTCAGCAGC CAGCACCCCA TGCCTGACAC CACTGTTAGT 
GGAGACACAG AAACGGGCCG GAGTCCTGGT GTCTTCAACA CTAAGGAATC TGGCGTGAAG 
GACATCAAGT CTGAATTGGC TGAAGTCAAA GATACTCTAA AGAACAAAAG TGACAAGCTG 
GAAGAGCTGG ATGGAAAAGT GAAGGGCTAC GAAGGGCAGC TCAGACAGCT CCAGGAAGCA 
GCCCAGGGCC CGACGGTGAC CATGACAACC AACGAACTCT ACCAAGCCTA TGTGGACAGT 
AAGATCGACG CCCTGAGAGA GGAGCTCATG GAGGGCATGG ACAGAAAGCT GGCTGACCTG 
AAAAACTCAT GTGAGTACAA GCTCACTGGC CTCCCGCAGC AGTGTGATGA CTATGGGAGC 
AGCTACCTGG GAGTGATAGA GCTCATAGGG GAGAAGGAAA CAAGCCTGAG AAAAGAAATA 
AATAACCTCC GAGCCCGGCT ACAGGAGCCT TCAGCCCAGG CAAATTGCTG CGACAGTGAA 
AAGAATGGTG ACATTGGTCA ACAGATCAAA ACATTGGACC AGAAAATTGA GAGAGTTGCT 
GAAGCCACCA GAATGCTGAA TGGAAGACTG GACAATGAGT TTGACCGCCT TATAGTTCCA 
GAGCCAGATG TGGATTTTGA TGCAAAATGG AATGAACTCG ATGCAAGGAT CAACGTGACG 
GAGAAGAACG CTGAAGAACA TTGCTTTTAC ATTGAGGAAA CCCTTCGGGG GACCATTAAT 
GGAGAGGTGG GTGACTTGAA GCAGCTTGTT GATCAGAAAA TACAGTCTCT GGAAGACCGT 
CTGGGGAGCG TTCTCCTACA GATGGCCAAC AACACTGGTG CAGAGCTCAG TCCCCCAGGG 
GCAGCAGCCC TGCCAGGAGT GTCAGGGTCA GGAGGTGAAC GGGTCATGAT GGAATTAAAC 
CACCTGAAGG ACAAAGTTCA AGTTGTTGAA GACATTTGCC TGCAGAACAT CCAGGGAAAG 
CCTCATGGGA TGGAAGGTGC CTTGCCAAAC AGGGAAGACC GCACCGTACG CGACAGCCTG 
CACCTTTTGA AATCTCTCAA CGACACGATG CACAGGAAGT TTCGAGAAAC TGAACGAACC 
ATCCAGAAAC TTCAGCAGGA TTTTAGTTTT CTTTATTCTC AATTAAACCA CACAGAAAAT 
GATGTGACTC GTCTTCAGAA GGAAATGAGC AATTGTAGAG CAGGTGAAAA TGCTGGCATG 
GGTGGGTTCA CTAAGGTGGA TGAGCAAGAA AGGACAGTGG ACACCCTGCC ATCCCCCCAG 
CACCCCACGG CTCATTGCTG CAGTCAGCTG GAGGAGAGGT GGCAGAGGTT GCAGAGCCAG 
GTCATCTCGG AGCTGGATGC TTGTAAGGAA AGCACGCAGG GGGTCCAGAG GGAGGTCTCC 
ATGGTGGAGG GCAGGGTGTC TCATATGGAG AAAACTTGCA GCAAGCTGGA CTCTATCTCA 
GGAAATCTTC AGAGGATCAA GGAGGGGCTC AACAAGCATG TCAGCAGCCT GTGGAACTGT 
GTCAGGCAGA TGAACGGAAC GCTCAGGTCG CATTCCAGAG ACATTTCTGG CCTGAAGAAT 
TCAGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTA CTGATTTGCA AGATCTGGTC 
AAATTTCAAC CATCAGCAAC CCGAGGGATA CTTTTACATG CCTACTTGTC AGCCACCTTG 
GTGGCCTGCC CTCCTGTCCC GCGGGGGAGA CCAACAACTG TTTCTTCGAA AAAGCCCCTT 
TATCTAAGCT CACCCCGGGC CCATTCAGCA CCACAAGCGA CAGTTTCTCT GAATTTTCTG 
ATGAGTCCAG CATTTCTCAT GCTTCAGTCC GTGATGACTG ATGGGGGCGG GGGGGATGGC 
GTTTCTTTGC GAGTGATTCC TCAGCCAGAC CCGGGTATTT TTAGGCGTCC TGTGGACGGT 
CGGGAGGGGC TTCCTGGAGG GCTAGTTCCA GGAACGAATT CAAAGGAGGA AAGGGCGGCA 
GCTTCCGGCT GCTTCCCAAG AGGGCCGGGA AATGTAAATC AGGAGGTTCC TCCGTCGGAA 
GTCCCTTCGG CTGGCGACGT GAGCGCTTTG GCTGTGGCCC CCGTGGAGCC TGAGCCCTCC 
GCACGGCCTC AGCGCAGCCT TGGGGCCTCC CGGCAGGGTC CCTCGGCAGC CCGCGGGAGA 
GGCCGTGGGG CCCTGGGAGG AGCGTGGTTC AGAGTCTGTG AGGGGGCAGG GACTCAAGCA 
GCAGCAGGAG CGTCTAGGCG GGGTNAGGAG CGGGGCGTGG ATGGCCAGAC GAAGAAATAT 
AAAATCCCCA GCGCAGAAGG CTTTGCGGGC GCACCAGGAT ACCCGAAGTC ACCTCCTGTA 
GCTTCCCCAG GAGCTCCGGT GCCTTCTCTG GTGTCTTTTT CTGCGGGGCT CACCCAGAAG 
CCTTTCCCCA GCGATGGGGG AGTTGTCCTC TTTAACAAAG TGCTGGTGAA CGACGGGGAC 
GTTTACAATC CCAGCACGGG GGTCTTCACG GCTCCTTATG ATGGGCGTTA CCTGATCACG 
GCGACCCTCA CCCCCGAGAG AGACGCCTAC GTGGAAGCAG TGCTGTCAGT CTCCAACGCC 
AGCGTGGCCC AGCTGCATAC CGCTGGGTAC AGGAGAGAGT TCCTGGAATA CCACCGCCCT 
CCAGGGGCTT TGCACACCTG CGGGGGCCCA GGGGCATTCC ACCTCATCGT GCACCTGAAG 
GCGGGAGATG CAGTCAACAT CGTGGTGACT GGGGGCAAGC TGGCTCACAC AGACTTTGAC 
GAAATGTACT CCACATTTAG TGGGGTTTTC TTATATCCTT TCCTTTCCCA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EMILIN2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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