PF3D7_1300100 cDNA ORF clone, Plasmodium falciparum 3D7

Gene
Clones
gRNAs

Summary

Gene Symbol	PF3D7_1300100
Entrez Gene ID	813647
Full Name	erythrocyte membrane protein 1, PfEMP1
Gene Type	protein-coding
Organism	Plasmodium falciparum 3D7

mRNA and Protein(s)

mRNA	Protein	Name
XM_001349702.1	XP_001349738.1	erythrocyte membrane protein 1, PfEMP1

Pathways from BioSystems
Gene Ontology

The following PF3D7_1300100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PF3D7_1300100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr03318	XM_001349702.1 Latest version!	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_1300100), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PF3D7_1300100 ( XM_001349702.1 ) cDNA ORF clone, Plasmodium falciparum 3D7 -> XP_001349738.1 Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_1300100), partial mRNA.

Price & Availability↑

CloneID	OPr03318
Clone ID Related Accession (Same CDS sequence)	XM_001349702.1
Accession Version	XM_001349702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522080000000
Organism	Plasmodium falciparum 3D7
Product	erythrocyte membrane protein 1, PfEMP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004331). COMPLETENESS: incomplete on both ends.

ATGGGGCCGC CAGGTATTAC TGGTACGCAG GGTGAAACTG CCAAACATAT GTTTGATAGG 
ATAGGGAAAC AAGTGTACGA AACAGTGAAA AATGAAGCTG AAAATTATAT TAGTGAATTG 
GAAGGAAAGT TGTCACAAGC AACACTTTTG GGGGAAAGAG TTAGCAGCCT TAAAACATGC 
CAACTTGTAG AGGATTATCG TAGTAAGGCT AATGGTGATG TTAAAAGGTA TCCGTGCGCT 
AATAGATCAC CAGTTCGTTT TTCCGATGAA AGTCGAAGTC AATGTACATA TAATAGAATA 
AAAGATAATG AAACCGATGA TAATGCATGT GGCGCCTGCG CGCCGTATCG ACGTTTACAT 
TTATGTGATT ATAATTTGGA AAAAATGGGC AAAACGTCGA CAACGAAGCA TGATTTGTTG 
GCAGAAGTAT GTATGGCAGC AAAATACGAA GGGGACTCAA TAAAAACACA TTATACAATA 
CATAAACACA CAAATAATGA TTCTGCTGCT GAATTATGTA CTGAGTTGGC ACGAAGTTTT 
GCAGATATTG GAGACATTAT ACGAGGAAAA GATCTGTATC TCGGTGATAT AAAAAAAAAG 
CAAAATGGAA AAAAAACAGA AAGAGAAAAA TTAGAAGAGA ATTTAAAAAG AATTTTCGGG 
AAAATACATG AGGACGTGAC GAATGGGAAG AAGGAGGTGC TAAAAACACG CTACAAAGAT 
ATTAATGATC CAGAATTTTT TAAATTACGA GAAGATTGGT GGACTGCGAA TCGCGCCACA 
GTATGGAAAG CCATCACATG TCATGCAGGA GAAAGTGATA AATATTTTCG AAACACATGC 
AATGATAGTG AACATAGTGG AACTTTTTCT CAACCTAATA AATACTGCCG GTGTAACGGC 
GACAAACCAG GTGAAGACAA GGCAAATGTC GATCCCCCAA CCTATTTTGA TTATGTGCCG 
CAGTATCTTC GCTGGTTCGA GGAATGGGCA GAAGATTTTT GTAGAATAAG GAAACATAAA 
TTAAAAAATG CTAAAGAACA ATGTCGTGAA AAATATAAAA GTGGTACGGA TCGATATTGT 
AGCCGTAATG GCTACGATTG CACACAAACT ATTAGAGGAA GAAACATTCT TGTCTCCGAT 
TCGGAATGTA CTAACTGCTC TGTTGTATGC ACTCCTTTTG TAAAATGGAT AGAGAACAAA 
AAACTGGAAT TTGAAAAACA AAAAGGAAAA TATACAAAAG AAATAGAAAA AGCAAACGGA 
ACATCAAACG GAACAACAAT AAGAACTCAA TATGGAACAA TTAATAATAT GTATAGAAAA 
GATTTTTATC AACAACTACA AAGTGGCTAT GGAGATGTCA ATGCTTTTTT AGAATTGTTA 
AATAAAGAAA CAACATGCAA AGATCACCCT AAAGTGGAAG AAAAATCTGA TATTGATTTC 
AATGAAGGTA CTGAAAAAAC ATTTTCTCAT ACGGAATATT GCGAAACCTG TCCGTGGTGT 
GCTACCAAAA AAAAAGGAAT TGATGGAAAC TGGGAAGAAC AAAAGTATGA AGAAGGATGC 
GAAAACTATT TAATGAAACC CATTGATGAA AGTAAAAGTA CTGATATTGA TTTGCTTGTC 
AAGGATACGT CAGGAACAAC TATGGTGGAG AAACTAGGAG GTTTGTGTAA CGATTCTTCT 
AAAAGAACTG TTCAAATGGA AAATTGGCAA TGTCATTATG AAAAAAAAAG TCAATATGAA 
GATGGTTTTG ATAAGGATTA TTGTGTACTA AAAGATGACA AAAAAAAAAA ACCAGAACAT 
AGGACAATTA AATCCTATTA TACCTTATTT CCAAATTGGA TAAATGAAAT GTTAAAAGAT 
TCGATAGATT GGAGTAAAGA ACTTAAAACC TGTATAAATA ATGAAAAACC AACTAATTGT 
ATACGTGAGT GTAAAAGTAA ATGCGATTGT TTTAAAAAAT GGGTTGTACA AAAAGAACAA 
GAATGGAAAC AATTAGAAGA ACACTATGAA AAAGAAAATT TTAGTGGAGA TTTTGGTCCA 
AGAATTAGTC CTTATGTGAC TCTTGAAGGG AATTTACAAT ACAGTTATTT GGAAATGATT 
CGAAAGTATT ATGCACAAGA AAAACCCGTA CAAGAAATTG AACAAATAAT TGAAAAAAAT 
AAGAACAATT TCGAAGTTAA AGAAGATGAT AATTCAATAA CCAAGTTCTT ACAGCAAGAA 
AAAGGAATTG CCACCAAATG CATAGAAAAA CAGGAAGAAT GCAAGCAACA AAAAAAACAA 
CAACGACAAA AACAACCCGC CGATAAAGTT GTCTCCCGCT CCGGCGCCTC ACCCGACACC 
GCCTCACCCG ACACTAAGGC CACTGAGGAA AAGGAAGAAG AAGAAGAGGA AGAAGAAGAA 
GAGGACCTCG GCGAGGAGTC GGAGGAGCCG GAGGACCAAG CGGTGGTAGA CCAGGATGGG 
CAGGGGGAGA CCACAGAAAA GAAGGTACCG GCGACCACAG AAGAGGGATC ACCAAAAGAA 
ACAACAACAC CAGAGAAGTC AGTAGACGTT TGCAAAACGG TGGCCGAACT ATTTAGTAAC 
GTGGACAATC TGAAAGAAGC CTGCACACAA AAATATGGCG GAAATAACTC ACGTTTAGGT 
TGGAAGTGCA TACCAACTAG TGGTGGTGAA AAAGCCACAG GTGGTAGTGG TGAATCCACT 
GGTAGTGATG CCACCACCGG TGGTAGTATT TGTGTGCCAC CGCGAAGACG ACGATTATAT 
GTCACACCAC TAACGAAGTG GGCAACTAAC ATGGAGGCGA CAGAAGCACA AGCGGGTGGT 
GACGAGGCCA CGGAGGCGTC AGTACCACTA GGTGACGGCG TGTCGAAAAA CCCCAAAGAG 
GCACTACTCA AAGCATTTGT TGAGTCTGCA GCGGTAGAAA CTTTTTTCTT GTGGCATAGA 
TATAAAAAAA TAAAAGACAA AGAGAAAAAA GAAAAAGAAG AACGAGAACG GGAAAATGCA 
GGACTTGACC CCTTCGGCGG TAGCGTCGAC GATGAGGCAT CTACACCTGA CCCTCAAACA 
CAATTACAAA GTGGTACCAT ACCCCCCGAT TTTTTGCGAC AGATGTTTTA TACATTAGGT 
GATTATAGAG ATATATGCAT AGGTGGTGAC CGCGATATCG TGGGCGACAC TATTGTTAGT 
AACAAAGAGG GTGGTACCCC TACAAAAATA TCGGATAAAA TAAAAGAAAT TTTAGAAAAA 
GTTGACAAAA AACAACCTGA TAAACAAAAT AGTGGCACAA CCCCTAAAAC CTGGTGGGAA 
GAAAATGGTC CTCATATCTG GAATGCTATG GTATGTGCTT TAACATATAA AGATAATGGC 
GACAAAGGCA AACCACAAGT GGATGACACT GTGCACTCAC AACTTTGGGA CACCACCAAG 
AACAAACCCA AAAACCGCCA ATACGAATAC GACCAAGTCA AACTCGACGA AAATAGTGGT 
ACCGGCCCCA AAAGCAACGA CCCCATCAAC AACCCCACGT TAAAAGAGTT TATTTCTCGC 
CCCCCCTACT TCCGATACCT TGAAGAATGG GGAGAAACAT TTTGTCGACA AAGGGCGCGG 
ATGTTGAAAC AAGTGGAAAA AAATTGCACA CAACATGGTA AAAAACAATA TAGTGGGGAT 
GGGGAATATT GTGACAAGAT TCATAAGGAT CCTACTACAC TTCCGGATTT AGGTTACAGT 
TGTCCCAAAT CTTGTAGTTC TTATAGAAAA TGGATAGAAA GAAAAAAAAC ACAATATGAG 
AAACAAGAAA AGGCATATAA CAATCAAAAA GCGAATGTTC AAAATAATAA TGATAATGGA 
TTTTGTGGAA CACTAGAAAG GGAGGCTGCA GCATTTTTAA ATAGCTTAAA AAACGGACCA 
TGTAAAAAGG AGAATGGAAA GGATAATGAT GAGTATGAAA TAAAATTTGA TGATAAAGAG 
AAAACATTTA AACACGCAAA ATATTGTGAT CCATGTCCTA AATTTAAAGT TGATTGTAAA 
AATGGTAAAT GCGATAATGA TAAAGGAGGG GATTGCAATG GTATAAAGAC TATTGATGCA 
AAAAATTTTG AAACAATGGT AAAACCTACT GAAGAAATTG TTATGCGTGT GAGTGATAAT 
AGTGGAAATA AATTTAATGA TTTAAGCGAT TGTCAAAATG CAGGTATATT TGAAGGCATT 
AAAGAAAATA AATATAAATG TGGTAATGTA TGTGGTTATG TTGTATGTAA ATCGGAAAAT 
GGCAATGGGG AAAAAGTCAA TGGGATAGAA AATCAAAACA AAATTATAAC AATTAGAGGT 
TTGGTTGCAC ATTGGGTACA ATATTTTTTA GAAGATTATA ATAAAATTAA ACATAAAATT 
TCACATTGTA TAAATAATGG TGAGGTATCC AAATGTATAA AAGATTGTGT GAAAAAATGG 
GTAGAAGAAA AAGAGAAAGA ATGGAAAAAA CTAAAAGAAC ATTACCAAAA ACAATATGGT 
TATAATAATT CAGGGGAATC TTACCCAGTG AGAAGTATTT TGGAGCAATT CCAATCTGGA 
ACTGAATTTA AAAACGCTAT AAAACCTTGT GGAACATTAC AACAGTTCGA GAGTTTTTGT 
GGTCTTAATG GCGATGAGAG CTCAAAAAAG GAAAATGGTA ATGAGGATGC GGTACAATGT 
TTGCTTAAAA ATCTTGGCAA TGAAGCTAAA AAGTGTGAAG AGAAACAACA AACTAGTGGC 
ATCCCCGAGG CACCGTGTGT TAACCACACC CCCCTTGAAG ATGACGATGA GGAACCCTAT 
GAGGACTTAC TCCTTCAAGA AACAGAAGAA AAACCAGAGG AGGCAAAAAA GAACATGATG 
CCGAAAATTT GTAAAGATGT GGTACAAGAA GCAGAAACAG TGGTAGAAAG CGGTTGTGTC 
CCAGCAAAAA CACCGGAAGC ACCAGCGGCA TCACCAGCAC CAAAAGAACC ACCAGCACCT 
AGTGAAGGCA CAAAGGAACG TCCATCTCCA CCAGAAAAAA AAGCACCGTT ACCAAAAGAA 
GAGAAAACCA AACCAAAACG ATCACTAACC CCAACAGACG ACCCATGGGA ACCCCTGAAA 
AATGCCATGT TATCTTCGAC GATCATGTGG AGTATTGGCA TTGGTTTTGC TACATTCACT 
TATTTTTATC TAAAGAAAAA AACTAAACGC CTTGTGGACC TTTTCAGTGT TATTAATATC 
CCCAAAAGTG ATTATGATAT ACCGACAAAA CTTTCACCCA ATAGATATAT ACCTTATACT 
AGTGGTAAAT ACAGAGGCAA ACGGTACATT TACCTTGAAG GAGATAGTGG AACAGATAGT 
GGTTACACCG ATCATTATAG TGATATAACT TCCTCTTCCG AAAGTGAATA TGAAGAATTG 
GATATTAATG ATATATATGT ACCAGGTAGT CCTAAATATA AAACGTTGAT AGAAGTGGTA 
CTAGAACCTA GTGGTAACAA CACAACAGCT AGTGGTAAAA ACACACCTAG TGATACACAA 
AATGATATAC AAAGTGATGA TATACCTAGT AGTAAAATTA CAGATAATGA GTGGAATACA 
TTGAAAGATG ATTTTATATA TAATATGTTA CAAAATGAAC CAAATGATAT ACCAAATGAT 
TATACAAGTG GAGATATTCC TTTAAACACA CAACCGAATA CTTTATATAT TGATAAACCT 
GATGAGAAAC CTTTTATTAC TTCTATTCAT GATAGAAATT TATATACTGG AGAAGAATAT 
AGTTATAATG TTAATATGGT TAATAGTATG GATGATATTC CAATTAATCG TGATAATAAT 
GTTTATAGTG GTATAGATTT AATTAATGAC ACGTTAAGTG GTAACCATAA TGTTGATATT 
TATGATGAAG TTTTGAAAAG AAAAGAAAAT GAATTATTTG GAATAAATCA TAAAAAACAT 
ACAACAATTA ACCGTTTCGC CAAACCTGCA CGTGACGACC CCCTACACAA CCAACTAAAT 
TTGTTTCATA CATGGTTAGA TAGACATAGA GATATGTGTG AACAATGGAA TAATAAAGAG 
GAAGTATTAG ATAAATTAAA AGAAGAGTGG GAAAATGAGA CACATAGTGG TAACACTCAC 
CCTAGTGATA GTAACAAAAC GTTGAATACT GATGTTTCTA TACAGATAGA TATGAATAAT 
CCCAAAACTA CAAATGAGTT TACATATGTG GATAGTAACC CCAACCAAGT GGATGACACC 
TACGTGGATA GTAACCCTGA CAATTCTTCC ATGGATACTA TCTTGGAGGA TCTGGACAAA 
CCATTTAATG AACCCTACTA TTATGATATG TATGACGATG ATATTTATTA TGATGTAAAT 
GATGATAATG ATATATCAAC TGTGGATACT AATGCTATGG ATGTTCCTAG TAAAGTACAA 
ATTGAAATGG ATGTAAATAC CAAATTAGTG AAAGAGAAAT ATCCTATATC GGATGTGTGG 
GATATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001349738.1
CDS	1..6489
Translation	MGPPGITGTQGETAKHMFDRIGKQVYETVKNEAENYISELEGKLSQATLLGERVSSLKTCQLVEDYRSKANGDVKRYPCANRSPVRFSDESRSQCTYNRIKDNETDDNACGACAPYRRLHLCDYNLEKMGKTSTTKHDLLAEVCMAAKYEGDSIKTHYTIHKHTNNDSAAELCTELARSFADIGDIIRGKDLYLGDIKKKQNGKKTEREKLEENLKRIFGKIHEDVTNGKKEVLKTRYKDINDPEFFKLREDWWTANRATVWKAITCHAGESDKYFRNTCNDSEHSGTFSQPNKYCRCNGDKPGEDKANVDPPTYFDYVPQYLRWFEEWAEDFCRIRKHKLKNAKEQCREKYKSGTDRYCSRNGYDCTQTIRGRNILVSDSECTNCSVVCTPFVKWIENKKLEFEKQKGKYTKEIEKANGTSNGTTIRTQYGTINNMYRKDFYQQLQSGYGDVNAFLELLNKETTCKDHPKVEEKSDIDFNEGTEKTFSHTEYCETCPWCATKKKGIDGNWEEQKYEEGCENYLMKPIDESKSTDIDLLVKDTSGTTMVEKLGGLCNDSSKRTVQMENWQCHYEKKSQYEDGFDKDYCVLKDDKKKKPEHRTIKSYYTLFPNWINEMLKDSIDWSKELKTCINNEKPTNCIRECKSKCDCFKKWVVQKEQEWKQLEEHYEKENFSGDFGPRISPYVTLEGNLQYSYLEMIRKYYAQEKPVQEIEQIIEKNKNNFEVKEDDNSITKFLQQEKGIATKCIEKQEECKQQKKQQRQKQPADKVVSRSGASPDTASPDTKATEEKEEEEEEEEEEDLGEESEEPEDQAVVDQDGQGETTEKKVPATTEEGSPKETTTPEKSVDVCKTVAELFSNVDNLKEACTQKYGGNNSRLGWKCIPTSGGEKATGGSGESTGSDATTGGSICVPPRRRRLYVTPLTKWATNMEATEAQAGGDEATEASVPLGDGVSKNPKEALLKAFVESAAVETFFLWHRYKKIKDKEKKEKEERERENAGLDPFGGSVDDEASTPDPQTQLQSGTIPPDFLRQMFYTLGDYRDICIGGDRDIVGDTIVSNKEGGTPTKISDKIKEILEKVDKKQPDKQNSGTTPKTWWEENGPHIWNAMVCALTYKDNGDKGKPQVDDTVHSQLWDTTKNKPKNRQYEYDQVKLDENSGTGPKSNDPINNPTLKEFISRPPYFRYLEEWGETFCRQRARMLKQVEKNCTQHGKKQYSGDGEYCDKIHKDPTTLPDLGYSCPKSCSSYRKWIERKKTQYEKQEKAYNNQKANVQNNNDNGFCGTLEREAAAFLNSLKNGPCKKENGKDNDEYEIKFDDKEKTFKHAKYCDPCPKFKVDCKNGKCDNDKGGDCNGIKTIDAKNFETMVKPTEEIVMRVSDNSGNKFNDLSDCQNAGIFEGIKENKYKCGNVCGYVVCKSENGNGEKVNGIENQNKIITIRGLVAHWVQYFLEDYNKIKHKISHCINNGEVSKCIKDCVKKWVEEKEKEWKKLKEHYQKQYGYNNSGESYPVRSILEQFQSGTEFKNAIKPCGTLQQFESFCGLNGDESSKKENGNEDAVQCLLKNLGNEAKKCEEKQQTSGIPEAPCVNHTPLEDDDEEPYEDLLLQETEEKPEEAKKNMMPKICKDVVQEAETVVESGCVPAKTPEAPAASPAPKEPPAPSEGTKERPSPPEKKAPLPKEEKTKPKRSLTPTDDPWEPLKNAMLSSTIMWSIGIGFATFTYFYLKKKTKRLVDLFSVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDSGYTDHYSDITSSSESEYEELDINDIYVPGSPKYKTLIEVVLEPSGNNTTASGKNTPSDTQNDIQSDDIPSSKITDNEWNTLKDDFIYNMLQNEPNDIPNDYTSGDIPLNTQPNTLYIDKPDEKPFITSIHDRNLYTGEEYSYNVNMVNSMDDIPINRDNNVYSGIDLINDTLSGNHNVDIYDEVLKRKENELFGINHKKHTTINRFAKPARDDPLHNQLNLFHTWLDRHRDMCEQWNNKEEVLDKLKEEWENETHSGNTHPSDSNKTLNTDVSIQIDMNNPKTTNEFTYVDSNPNQVDDTYVDSNPDNSSMDTILEDLDKPFNEPYYYDMYDDDIYYDVNDDNDISTVDTNAMDVPSKVQIEMDVNTKLVKEKYPISDVWDI

Target ORF information:

RefSeq Version	XM_001349702.1
Organism	Plasmodium falciparum 3D7
Definition	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_1300100), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001349702.1

ORF Insert Sequence:

ATGGGGCCGC CAGGTATTAC TGGTACGCAG GGTGAAACTG CCAAACATAT GTTTGATAGG 
ATAGGGAAAC AAGTGTACGA AACAGTGAAA AATGAAGCTG AAAATTATAT TAGTGAATTG 
GAAGGAAAGT TGTCACAAGC AACACTTTTG GGGGAAAGAG TTAGCAGCCT TAAAACATGC 
CAACTTGTAG AGGATTATCG TAGTAAGGCT AATGGTGATG TTAAAAGGTA TCCGTGCGCT 
AATAGATCAC CAGTTCGTTT TTCCGATGAA AGTCGAAGTC AATGTACATA TAATAGAATA 
AAAGATAATG AAACCGATGA TAATGCATGT GGCGCCTGCG CGCCGTATCG ACGTTTACAT 
TTATGTGATT ATAATTTGGA AAAAATGGGC AAAACGTCGA CAACGAAGCA TGATTTGTTG 
GCAGAAGTAT GTATGGCAGC AAAATACGAA GGGGACTCAA TAAAAACACA TTATACAATA 
CATAAACACA CAAATAATGA TTCTGCTGCT GAATTATGTA CTGAGTTGGC ACGAAGTTTT 
GCAGATATTG GAGACATTAT ACGAGGAAAA GATCTGTATC TCGGTGATAT AAAAAAAAAG 
CAAAATGGAA AAAAAACAGA AAGAGAAAAA TTAGAAGAGA ATTTAAAAAG AATTTTCGGG 
AAAATACATG AGGACGTGAC GAATGGGAAG AAGGAGGTGC TAAAAACACG CTACAAAGAT 
ATTAATGATC CAGAATTTTT TAAATTACGA GAAGATTGGT GGACTGCGAA TCGCGCCACA 
GTATGGAAAG CCATCACATG TCATGCAGGA GAAAGTGATA AATATTTTCG AAACACATGC 
AATGATAGTG AACATAGTGG AACTTTTTCT CAACCTAATA AATACTGCCG GTGTAACGGC 
GACAAACCAG GTGAAGACAA GGCAAATGTC GATCCCCCAA CCTATTTTGA TTATGTGCCG 
CAGTATCTTC GCTGGTTCGA GGAATGGGCA GAAGATTTTT GTAGAATAAG GAAACATAAA 
TTAAAAAATG CTAAAGAACA ATGTCGTGAA AAATATAAAA GTGGTACGGA TCGATATTGT 
AGCCGTAATG GCTACGATTG CACACAAACT ATTAGAGGAA GAAACATTCT TGTCTCCGAT 
TCGGAATGTA CTAACTGCTC TGTTGTATGC ACTCCTTTTG TAAAATGGAT AGAGAACAAA 
AAACTGGAAT TTGAAAAACA AAAAGGAAAA TATACAAAAG AAATAGAAAA AGCAAACGGA 
ACATCAAACG GAACAACAAT AAGAACTCAA TATGGAACAA TTAATAATAT GTATAGAAAA 
GATTTTTATC AACAACTACA AAGTGGCTAT GGAGATGTCA ATGCTTTTTT AGAATTGTTA 
AATAAAGAAA CAACATGCAA AGATCACCCT AAAGTGGAAG AAAAATCTGA TATTGATTTC 
AATGAAGGTA CTGAAAAAAC ATTTTCTCAT ACGGAATATT GCGAAACCTG TCCGTGGTGT 
GCTACCAAAA AAAAAGGAAT TGATGGAAAC TGGGAAGAAC AAAAGTATGA AGAAGGATGC 
GAAAACTATT TAATGAAACC CATTGATGAA AGTAAAAGTA CTGATATTGA TTTGCTTGTC 
AAGGATACGT CAGGAACAAC TATGGTGGAG AAACTAGGAG GTTTGTGTAA CGATTCTTCT 
AAAAGAACTG TTCAAATGGA AAATTGGCAA TGTCATTATG AAAAAAAAAG TCAATATGAA 
GATGGTTTTG ATAAGGATTA TTGTGTACTA AAAGATGACA AAAAAAAAAA ACCAGAACAT 
AGGACAATTA AATCCTATTA TACCTTATTT CCAAATTGGA TAAATGAAAT GTTAAAAGAT 
TCGATAGATT GGAGTAAAGA ACTTAAAACC TGTATAAATA ATGAAAAACC AACTAATTGT 
ATACGTGAGT GTAAAAGTAA ATGCGATTGT TTTAAAAAAT GGGTTGTACA AAAAGAACAA 
GAATGGAAAC AATTAGAAGA ACACTATGAA AAAGAAAATT TTAGTGGAGA TTTTGGTCCA 
AGAATTAGTC CTTATGTGAC TCTTGAAGGG AATTTACAAT ACAGTTATTT GGAAATGATT 
CGAAAGTATT ATGCACAAGA AAAACCCGTA CAAGAAATTG AACAAATAAT TGAAAAAAAT 
AAGAACAATT TCGAAGTTAA AGAAGATGAT AATTCAATAA CCAAGTTCTT ACAGCAAGAA 
AAAGGAATTG CCACCAAATG CATAGAAAAA CAGGAAGAAT GCAAGCAACA AAAAAAACAA 
CAACGACAAA AACAACCCGC CGATAAAGTT GTCTCCCGCT CCGGCGCCTC ACCCGACACC 
GCCTCACCCG ACACTAAGGC CACTGAGGAA AAGGAAGAAG AAGAAGAGGA AGAAGAAGAA 
GAGGACCTCG GCGAGGAGTC GGAGGAGCCG GAGGACCAAG CGGTGGTAGA CCAGGATGGG 
CAGGGGGAGA CCACAGAAAA GAAGGTACCG GCGACCACAG AAGAGGGATC ACCAAAAGAA 
ACAACAACAC CAGAGAAGTC AGTAGACGTT TGCAAAACGG TGGCCGAACT ATTTAGTAAC 
GTGGACAATC TGAAAGAAGC CTGCACACAA AAATATGGCG GAAATAACTC ACGTTTAGGT 
TGGAAGTGCA TACCAACTAG TGGTGGTGAA AAAGCCACAG GTGGTAGTGG TGAATCCACT 
GGTAGTGATG CCACCACCGG TGGTAGTATT TGTGTGCCAC CGCGAAGACG ACGATTATAT 
GTCACACCAC TAACGAAGTG GGCAACTAAC ATGGAGGCGA CAGAAGCACA AGCGGGTGGT 
GACGAGGCCA CGGAGGCGTC AGTACCACTA GGTGACGGCG TGTCGAAAAA CCCCAAAGAG 
GCACTACTCA AAGCATTTGT TGAGTCTGCA GCGGTAGAAA CTTTTTTCTT GTGGCATAGA 
TATAAAAAAA TAAAAGACAA AGAGAAAAAA GAAAAAGAAG AACGAGAACG GGAAAATGCA 
GGACTTGACC CCTTCGGCGG TAGCGTCGAC GATGAGGCAT CTACACCTGA CCCTCAAACA 
CAATTACAAA GTGGTACCAT ACCCCCCGAT TTTTTGCGAC AGATGTTTTA TACATTAGGT 
GATTATAGAG ATATATGCAT AGGTGGTGAC CGCGATATCG TGGGCGACAC TATTGTTAGT 
AACAAAGAGG GTGGTACCCC TACAAAAATA TCGGATAAAA TAAAAGAAAT TTTAGAAAAA 
GTTGACAAAA AACAACCTGA TAAACAAAAT AGTGGCACAA CCCCTAAAAC CTGGTGGGAA 
GAAAATGGTC CTCATATCTG GAATGCTATG GTATGTGCTT TAACATATAA AGATAATGGC 
GACAAAGGCA AACCACAAGT GGATGACACT GTGCACTCAC AACTTTGGGA CACCACCAAG 
AACAAACCCA AAAACCGCCA ATACGAATAC GACCAAGTCA AACTCGACGA AAATAGTGGT 
ACCGGCCCCA AAAGCAACGA CCCCATCAAC AACCCCACGT TAAAAGAGTT TATTTCTCGC 
CCCCCCTACT TCCGATACCT TGAAGAATGG GGAGAAACAT TTTGTCGACA AAGGGCGCGG 
ATGTTGAAAC AAGTGGAAAA AAATTGCACA CAACATGGTA AAAAACAATA TAGTGGGGAT 
GGGGAATATT GTGACAAGAT TCATAAGGAT CCTACTACAC TTCCGGATTT AGGTTACAGT 
TGTCCCAAAT CTTGTAGTTC TTATAGAAAA TGGATAGAAA GAAAAAAAAC ACAATATGAG 
AAACAAGAAA AGGCATATAA CAATCAAAAA GCGAATGTTC AAAATAATAA TGATAATGGA 
TTTTGTGGAA CACTAGAAAG GGAGGCTGCA GCATTTTTAA ATAGCTTAAA AAACGGACCA 
TGTAAAAAGG AGAATGGAAA GGATAATGAT GAGTATGAAA TAAAATTTGA TGATAAAGAG 
AAAACATTTA AACACGCAAA ATATTGTGAT CCATGTCCTA AATTTAAAGT TGATTGTAAA 
AATGGTAAAT GCGATAATGA TAAAGGAGGG GATTGCAATG GTATAAAGAC TATTGATGCA 
AAAAATTTTG AAACAATGGT AAAACCTACT GAAGAAATTG TTATGCGTGT GAGTGATAAT 
AGTGGAAATA AATTTAATGA TTTAAGCGAT TGTCAAAATG CAGGTATATT TGAAGGCATT 
AAAGAAAATA AATATAAATG TGGTAATGTA TGTGGTTATG TTGTATGTAA ATCGGAAAAT 
GGCAATGGGG AAAAAGTCAA TGGGATAGAA AATCAAAACA AAATTATAAC AATTAGAGGT 
TTGGTTGCAC ATTGGGTACA ATATTTTTTA GAAGATTATA ATAAAATTAA ACATAAAATT 
TCACATTGTA TAAATAATGG TGAGGTATCC AAATGTATAA AAGATTGTGT GAAAAAATGG 
GTAGAAGAAA AAGAGAAAGA ATGGAAAAAA CTAAAAGAAC ATTACCAAAA ACAATATGGT 
TATAATAATT CAGGGGAATC TTACCCAGTG AGAAGTATTT TGGAGCAATT CCAATCTGGA 
ACTGAATTTA AAAACGCTAT AAAACCTTGT GGAACATTAC AACAGTTCGA GAGTTTTTGT 
GGTCTTAATG GCGATGAGAG CTCAAAAAAG GAAAATGGTA ATGAGGATGC GGTACAATGT 
TTGCTTAAAA ATCTTGGCAA TGAAGCTAAA AAGTGTGAAG AGAAACAACA AACTAGTGGC 
ATCCCCGAGG CACCGTGTGT TAACCACACC CCCCTTGAAG ATGACGATGA GGAACCCTAT 
GAGGACTTAC TCCTTCAAGA AACAGAAGAA AAACCAGAGG AGGCAAAAAA GAACATGATG 
CCGAAAATTT GTAAAGATGT GGTACAAGAA GCAGAAACAG TGGTAGAAAG CGGTTGTGTC 
CCAGCAAAAA CACCGGAAGC ACCAGCGGCA TCACCAGCAC CAAAAGAACC ACCAGCACCT 
AGTGAAGGCA CAAAGGAACG TCCATCTCCA CCAGAAAAAA AAGCACCGTT ACCAAAAGAA 
GAGAAAACCA AACCAAAACG ATCACTAACC CCAACAGACG ACCCATGGGA ACCCCTGAAA 
AATGCCATGT TATCTTCGAC GATCATGTGG AGTATTGGCA TTGGTTTTGC TACATTCACT 
TATTTTTATC TAAAGAAAAA AACTAAACGC CTTGTGGACC TTTTCAGTGT TATTAATATC 
CCCAAAAGTG ATTATGATAT ACCGACAAAA CTTTCACCCA ATAGATATAT ACCTTATACT 
AGTGGTAAAT ACAGAGGCAA ACGGTACATT TACCTTGAAG GAGATAGTGG AACAGATAGT 
GGTTACACCG ATCATTATAG TGATATAACT TCCTCTTCCG AAAGTGAATA TGAAGAATTG 
GATATTAATG ATATATATGT ACCAGGTAGT CCTAAATATA AAACGTTGAT AGAAGTGGTA 
CTAGAACCTA GTGGTAACAA CACAACAGCT AGTGGTAAAA ACACACCTAG TGATACACAA 
AATGATATAC AAAGTGATGA TATACCTAGT AGTAAAATTA CAGATAATGA GTGGAATACA 
TTGAAAGATG ATTTTATATA TAATATGTTA CAAAATGAAC CAAATGATAT ACCAAATGAT 
TATACAAGTG GAGATATTCC TTTAAACACA CAACCGAATA CTTTATATAT TGATAAACCT 
GATGAGAAAC CTTTTATTAC TTCTATTCAT GATAGAAATT TATATACTGG AGAAGAATAT 
AGTTATAATG TTAATATGGT TAATAGTATG GATGATATTC CAATTAATCG TGATAATAAT 
GTTTATAGTG GTATAGATTT AATTAATGAC ACGTTAAGTG GTAACCATAA TGTTGATATT 
TATGATGAAG TTTTGAAAAG AAAAGAAAAT GAATTATTTG GAATAAATCA TAAAAAACAT 
ACAACAATTA ACCGTTTCGC CAAACCTGCA CGTGACGACC CCCTACACAA CCAACTAAAT 
TTGTTTCATA CATGGTTAGA TAGACATAGA GATATGTGTG AACAATGGAA TAATAAAGAG 
GAAGTATTAG ATAAATTAAA AGAAGAGTGG GAAAATGAGA CACATAGTGG TAACACTCAC 
CCTAGTGATA GTAACAAAAC GTTGAATACT GATGTTTCTA TACAGATAGA TATGAATAAT 
CCCAAAACTA CAAATGAGTT TACATATGTG GATAGTAACC CCAACCAAGT GGATGACACC 
TACGTGGATA GTAACCCTGA CAATTCTTCC ATGGATACTA TCTTGGAGGA TCTGGACAAA 
CCATTTAATG AACCCTACTA TTATGATATG TATGACGATG ATATTTATTA TGATGTAAAT 
GATGATAATG ATATATCAAC TGTGGATACT AATGCTATGG ATGTTCCTAG TAAAGTACAA 
ATTGAAATGG ATGTAAATAC CAAATTAGTG AAAGAGAAAT ATCCTATATC GGATGTGTGG 
GATATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PF3D7_1300100 cDNA ORF clone, Plasmodium falciparum 3D7

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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