JAG1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	JAG1
Entrez Gene ID	102974158
Full Name	jagged 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007101500.1	XP_007101562.1	protein jagged-1
XM_028498654.1	XP_028354455.1	protein jagged-1 isoform X2
XM_024123389.1	XP_023979157.1	protein jagged-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following JAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh111789	XM_007101500.1 Latest version!	Physeter catodon jagged 1 (JAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPh292162	XM_024123389.1 Latest version!	Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPh321162	XM_028498654.1 Latest version!	Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JAG1 ( XM_007101500.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007101562.1 Physeter catodon jagged 1 (JAG1), mRNA.

Price & Availability↑

CloneID	OPh111789
Clone ID Related Accession (Same CDS sequence)	XM_007101500.1
Accession Version	XM_007101500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	protein jagged-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006712866.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAACG TGAACGGGGA GCTGCAAAAC GGGAACTGCT GTGGCGGCGC CCGGAACCCG 
GGGGACCGCA AGTGCTCCCG CGACGAGTGT GACACGTACT TCAAAGTGTG CCTCAAGGAG 
TACCAGTCGC GCGTCACGGC CGGGGGGCCC TGCAGCTTTG GCTCGGGGTC CACGCCTGTC 
ATCGGGGGCA ACACCTTCAA CCTCAAGGCC AGTCGCGGCA ACGAACGCAA CCGCATCGTG 
CTGCCTTTCA GTTTTGCCTG GCCGAGGTCC TACACTTTGC TTGTGGAGGC GTGGGATTCC 
AGCAATGACA CTGTCCAACC CGACAGCATT ATTGAAAAGG CTTCTCACTC AGGCATGATC 
AACCCCAGCC GGCAGTGGCA GACGCTGAAG CAGAACACGG GGGTTGCCCA CTTTGAGTAT 
CAGATCCGCG TGACCTGTGA TGACTATTAC TATGGCTTCG GCTGCAATAA GTTCTGCCGA 
CCCAGAGATG ACTTCTTTGG CCACTATGCC TGCGACCAGA ATGGCAACAA AACTTGCATG 
GAAGGCTGGA TGGGCCCTGA ATGTAACAAA GCTATTTGCC GGCAGGGCTG TAGCCCCAAG 
CATGGGTCTT GCAAACTCCC AGGTGACTGC AGGTGCCAGT ATGGCTGGCA GGGCCTGTAC 
TGTGACAAGT GCATCCCACA CCCAGGGTGC GTCCACGGCA CCTGTAATGA GCCCTGGCAG 
TGTCTCTGTG AGACCAACTG GGGTGGCCAG CTCTGCGACA AAGATCTCAA CTACTGCGGG 
ACCCACCAGC CATGTCTCAA TGGGGGAACT TGTAGCAACA CGGGGCCTGA CAAATATCAG 
TGTTCATGCC CCGAGGGGTA CTCAGGACCC AACTGTGAAA TTGCGGAACA CGCCTGCCTC 
TCTGATCCCT GCCACAACAG AGGCAGCTGC AAGGAGACCT CCCTGGGGTT TGAGTGTGAG 
TGTTCTCCGG GCTGGACCGG CCCCACGTGC TCCACAAACA TTGATGACTG TTCTCCAAAT 
AACTGTTCCC ACGGGGGCAC CTGCCAGGAC TTGGTTAACG GATTTAAGTG TGTGTGCCCG 
CCCCAGTGGA CGGGCAAAAC GTGCCAGTTA GATGCAAATG AATGCGAGAC CAAACCTTGT 
GTAAATGCCA AATCCTGTAA GAATCTGATT GCCAGCTACT ACTGTGATTG CCTCCCGGGC 
TGGATGGGTC AGAATTGTGA CATAAATATT AATGACTGCC TCGGCCAATG TCAGAATGAC 
GCCTCCTGTC GGGATTTGGT TAATGGTTAT CGCTGTATCT GTCCACCTGG CTATGCAGGC 
GATCACTGTG AGACAGACAT CGATGAGTGT GCCAGCAACC CCTGTCTCAA CGGGGGTCAC 
TGTCAGAACG AAATCAACAG ATTCCAGTGT CTGTGTCCCA CTGGTTTCTC TGGAAACCTC 
TGTCAGCTGG ACATCGATTA TTGCGAGCCC AATCCCTGCC AGAACGGTGC CCAGTGCTAC 
AACCGTGCCA GCGACTATTT CTGCAAGTGT CCCGAGGACT ATGAAGGCAA GAACTGCTCC 
CACCTTAAAG ACCACTGCCG CACCACCCCC TGTGAAGTGA TTGACAGCTG CACGGTGGCC 
ATGGCCTCCA ACGACACGCC TGAAGGGGTG CGCTATATTT CCTCCAATGT CTGCGGTCCC 
CACGGGAAGT GCAAGAGTCA GTCGGGAGGC AAATTCACCT GTGACTGTAA CAAAGGCTTC 
ACTGGAACGT ACTGCCATGA AAATATTAAC GACTGTGAGA GCAACCCCTG TAAAAATGGT 
GGCACTTGCA TCGATGGTGT CAACTCCTAC AAGTGTATCT GCAGTGGCGG CTGGGAAGGC 
GCCTACTGTG AGACCAATAT TAACGACTGC AGCCAGAACC CCTGCCACAA CGGGGGCTCG 
TGTCGCGACC TGGTCAACGA CTTTTACTGC GACTGTAAAA ACGGGTGGAA AGGCAAGACC 
TGCCACTCTC GTGACAGCCA GTGTGACGAG GCTACGTGCA ACAATGGTGG CACCTGCTAT 
GACGAGGGGG ACGCTTTCAA GTGCATGTGT CCCGGAGGCT GGGAAGGAAC GACCTGTAAC 
ATAGCCCGCA ACAGTAGCTG CCTGCCTAAC CCCTGCCACA ATGGGGGCAC CTGTGTGGTC 
AACGGCGAGT CCTTCACGTG TGTCTGCAAA GAAGGCTGGG AGGGGCCCAT CTGTACGCAG 
AATACCAATG ACTGCAGTCC TCATCCCTGT TACAACAGTG GCACGTGCGT GGATGGAGAG 
AACTGGTACC GCTGTGAGTG CGCCCCCGGC TTCGCTGGGC CCGACTGCAG GATAAACATC 
AACGAATGCC AGTCTTCACC TTGTGCCTTC GGGGCAACCT GTGTGGATGA GATCAATGGG 
TACCGGTGTG TCTGCCCCCC AGGGCACAGT GGTGCTAAAT GCCAGGAAGT TTCAGGGAGA 
TCTTGCATCA CCGTGGGGAG CGTGATACCT GATGGGGCCA AGTGGGACGA TGACTGCAAC 
ACCTGCCAGT GCCTGGATGG GAGGATCGCC TGCTCGAAGG TGTGGTGTGG CCCCCGCCCT 
TGCTCGCTGC ACAAAGGGCA CAGCGAGTGC CCCAGCGGGC AGAGCTGCAT CCCCATCCTG 
GACGCCCAGT GCTTCGTGCG GCCCTGCACT GGTGTGGGTG AGTGCCGGTC CTCCAGCCTC 
CAGCCCGTGA AGACCAAGTG TGCCTCCGAC CCCTACTACC AGGACAACTG TGCCAACATC 
ACGTTCACCT TCAACAAGGA GATGATGTCG CCGGGTCTCA CCACAGAGCA TATTTGCAGC 
GAACTGCGGA ATTTGAATAT TTTGAAGAAT GTTTCTGCTG AATATTCAAT CTACATAGCT 
TGCGAGCCTT CCCCTTCTGC AAACAATGAA ATACACGTGG CCATTTCTGC TGAAGACATC 
CGGGATGATG GAAACCCAAT CAAGGAAATC ACCGACAAAA TTATAGATCT TGTTAGTAAA 
CGTGAAGGGA ACAGCTCACT GATCGCCGCT GTTGCGGAAG TGAGAGTTCA GAGGCGTCCT 
CTGAAGAACA GAACAGATTT CCTGGTGCCC CTGCTGAGCT CGGTCCTGAC CGTGGCCTGG 
GTCTGCTGCC TGGTGACCGC CTTCTACTGG TGCGTGCGCA AGCGGCGGAA GCCCGGCAGC 
CACGCGCGCT CGGCCTCTGA GGAGAGCACC ACCAACAACG TGCGGGAACA GCTGAACCAG 
ATCAAGAACC CCATCGAGAA GCACGGGGCC AACACAGTCC CCATCAAGGA CTACGAGAAC 
AAGAACTCCA AAATGTCCAA AATAAGGACA CACAACTCGG AGGTGGAAGA GGACGACATG 
GACAAGCACC AGCAGAAAGC CCGCTTCGCC AGGCAGGCCA CGTACACGCT GGTCGACAGA 
GAGGAGAAGT CCCCCCACGG CACGCCGGCC AAACACCCGA ACTGGACAAA CAAACAGGAC 
AACAGAGACC TGGAGAGCGC GCAGAGCCTG AACCGCATGG AGTACATCGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007101562.1
CDS	57..3590
Translation	MQNVNGELQNGNCCGGARNPGDRKCSRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNERNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTCNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECETKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCETDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCKNGGTCIDGVNSYKCICSGGWEGAYCETNINDCSQNPCHNGGSCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICTQNTNDCSPHPCYNSGTCVDGENWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRSCITVGSVIPDGAKWDDDCNTCQCLDGRIACSKVWCGPRPCSLHKGHSECPSGQSCIPILDAQCFVRPCTGVGECRSSSLQPVKTKCASDPYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKREGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWVCCLVTAFYWCVRKRRKPGSHARSASEESTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFARQATYTLVDREEKSPHGTPAKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	XM_007101500.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon jagged 1 (JAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007101500.1

ORF Insert Sequence:

ATGCAGAACG TGAACGGGGA GCTGCAAAAC GGGAACTGCT GTGGCGGCGC CCGGAACCCG 
GGGGACCGCA AGTGCTCCCG CGACGAGTGT GACACGTACT TCAAAGTGTG CCTCAAGGAG 
TACCAGTCGC GCGTCACGGC CGGGGGGCCC TGCAGCTTTG GCTCGGGGTC CACGCCTGTC 
ATCGGGGGCA ACACCTTCAA CCTCAAGGCC AGTCGCGGCA ACGAACGCAA CCGCATCGTG 
CTGCCTTTCA GTTTTGCCTG GCCGAGGTCC TACACTTTGC TTGTGGAGGC GTGGGATTCC 
AGCAATGACA CTGTCCAACC CGACAGCATT ATTGAAAAGG CTTCTCACTC AGGCATGATC 
AACCCCAGCC GGCAGTGGCA GACGCTGAAG CAGAACACGG GGGTTGCCCA CTTTGAGTAT 
CAGATCCGCG TGACCTGTGA TGACTATTAC TATGGCTTCG GCTGCAATAA GTTCTGCCGA 
CCCAGAGATG ACTTCTTTGG CCACTATGCC TGCGACCAGA ATGGCAACAA AACTTGCATG 
GAAGGCTGGA TGGGCCCTGA ATGTAACAAA GCTATTTGCC GGCAGGGCTG TAGCCCCAAG 
CATGGGTCTT GCAAACTCCC AGGTGACTGC AGGTGCCAGT ATGGCTGGCA GGGCCTGTAC 
TGTGACAAGT GCATCCCACA CCCAGGGTGC GTCCACGGCA CCTGTAATGA GCCCTGGCAG 
TGTCTCTGTG AGACCAACTG GGGTGGCCAG CTCTGCGACA AAGATCTCAA CTACTGCGGG 
ACCCACCAGC CATGTCTCAA TGGGGGAACT TGTAGCAACA CGGGGCCTGA CAAATATCAG 
TGTTCATGCC CCGAGGGGTA CTCAGGACCC AACTGTGAAA TTGCGGAACA CGCCTGCCTC 
TCTGATCCCT GCCACAACAG AGGCAGCTGC AAGGAGACCT CCCTGGGGTT TGAGTGTGAG 
TGTTCTCCGG GCTGGACCGG CCCCACGTGC TCCACAAACA TTGATGACTG TTCTCCAAAT 
AACTGTTCCC ACGGGGGCAC CTGCCAGGAC TTGGTTAACG GATTTAAGTG TGTGTGCCCG 
CCCCAGTGGA CGGGCAAAAC GTGCCAGTTA GATGCAAATG AATGCGAGAC CAAACCTTGT 
GTAAATGCCA AATCCTGTAA GAATCTGATT GCCAGCTACT ACTGTGATTG CCTCCCGGGC 
TGGATGGGTC AGAATTGTGA CATAAATATT AATGACTGCC TCGGCCAATG TCAGAATGAC 
GCCTCCTGTC GGGATTTGGT TAATGGTTAT CGCTGTATCT GTCCACCTGG CTATGCAGGC 
GATCACTGTG AGACAGACAT CGATGAGTGT GCCAGCAACC CCTGTCTCAA CGGGGGTCAC 
TGTCAGAACG AAATCAACAG ATTCCAGTGT CTGTGTCCCA CTGGTTTCTC TGGAAACCTC 
TGTCAGCTGG ACATCGATTA TTGCGAGCCC AATCCCTGCC AGAACGGTGC CCAGTGCTAC 
AACCGTGCCA GCGACTATTT CTGCAAGTGT CCCGAGGACT ATGAAGGCAA GAACTGCTCC 
CACCTTAAAG ACCACTGCCG CACCACCCCC TGTGAAGTGA TTGACAGCTG CACGGTGGCC 
ATGGCCTCCA ACGACACGCC TGAAGGGGTG CGCTATATTT CCTCCAATGT CTGCGGTCCC 
CACGGGAAGT GCAAGAGTCA GTCGGGAGGC AAATTCACCT GTGACTGTAA CAAAGGCTTC 
ACTGGAACGT ACTGCCATGA AAATATTAAC GACTGTGAGA GCAACCCCTG TAAAAATGGT 
GGCACTTGCA TCGATGGTGT CAACTCCTAC AAGTGTATCT GCAGTGGCGG CTGGGAAGGC 
GCCTACTGTG AGACCAATAT TAACGACTGC AGCCAGAACC CCTGCCACAA CGGGGGCTCG 
TGTCGCGACC TGGTCAACGA CTTTTACTGC GACTGTAAAA ACGGGTGGAA AGGCAAGACC 
TGCCACTCTC GTGACAGCCA GTGTGACGAG GCTACGTGCA ACAATGGTGG CACCTGCTAT 
GACGAGGGGG ACGCTTTCAA GTGCATGTGT CCCGGAGGCT GGGAAGGAAC GACCTGTAAC 
ATAGCCCGCA ACAGTAGCTG CCTGCCTAAC CCCTGCCACA ATGGGGGCAC CTGTGTGGTC 
AACGGCGAGT CCTTCACGTG TGTCTGCAAA GAAGGCTGGG AGGGGCCCAT CTGTACGCAG 
AATACCAATG ACTGCAGTCC TCATCCCTGT TACAACAGTG GCACGTGCGT GGATGGAGAG 
AACTGGTACC GCTGTGAGTG CGCCCCCGGC TTCGCTGGGC CCGACTGCAG GATAAACATC 
AACGAATGCC AGTCTTCACC TTGTGCCTTC GGGGCAACCT GTGTGGATGA GATCAATGGG 
TACCGGTGTG TCTGCCCCCC AGGGCACAGT GGTGCTAAAT GCCAGGAAGT TTCAGGGAGA 
TCTTGCATCA CCGTGGGGAG CGTGATACCT GATGGGGCCA AGTGGGACGA TGACTGCAAC 
ACCTGCCAGT GCCTGGATGG GAGGATCGCC TGCTCGAAGG TGTGGTGTGG CCCCCGCCCT 
TGCTCGCTGC ACAAAGGGCA CAGCGAGTGC CCCAGCGGGC AGAGCTGCAT CCCCATCCTG 
GACGCCCAGT GCTTCGTGCG GCCCTGCACT GGTGTGGGTG AGTGCCGGTC CTCCAGCCTC 
CAGCCCGTGA AGACCAAGTG TGCCTCCGAC CCCTACTACC AGGACAACTG TGCCAACATC 
ACGTTCACCT TCAACAAGGA GATGATGTCG CCGGGTCTCA CCACAGAGCA TATTTGCAGC 
GAACTGCGGA ATTTGAATAT TTTGAAGAAT GTTTCTGCTG AATATTCAAT CTACATAGCT 
TGCGAGCCTT CCCCTTCTGC AAACAATGAA ATACACGTGG CCATTTCTGC TGAAGACATC 
CGGGATGATG GAAACCCAAT CAAGGAAATC ACCGACAAAA TTATAGATCT TGTTAGTAAA 
CGTGAAGGGA ACAGCTCACT GATCGCCGCT GTTGCGGAAG TGAGAGTTCA GAGGCGTCCT 
CTGAAGAACA GAACAGATTT CCTGGTGCCC CTGCTGAGCT CGGTCCTGAC CGTGGCCTGG 
GTCTGCTGCC TGGTGACCGC CTTCTACTGG TGCGTGCGCA AGCGGCGGAA GCCCGGCAGC 
CACGCGCGCT CGGCCTCTGA GGAGAGCACC ACCAACAACG TGCGGGAACA GCTGAACCAG 
ATCAAGAACC CCATCGAGAA GCACGGGGCC AACACAGTCC CCATCAAGGA CTACGAGAAC 
AAGAACTCCA AAATGTCCAA AATAAGGACA CACAACTCGG AGGTGGAAGA GGACGACATG 
GACAAGCACC AGCAGAAAGC CCGCTTCGCC AGGCAGGCCA CGTACACGCT GGTCGACAGA 
GAGGAGAAGT CCCCCCACGG CACGCCGGCC AAACACCCGA ACTGGACAAA CAAACAGGAC 
AACAGAGACC TGGAGAGCGC GCAGAGCCTG AACCGCATGG AGTACATCGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAG1 ( XM_024123389.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023979157.1 Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh292162
Clone ID Related Accession (Same CDS sequence)	XM_024123389.1
Accession Version	XM_024123389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	protein jagged-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041227.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTCCC CACGGACGCG CGGCCGGCCC GGGCGCCCCC TGAGCCTCCT GCTCGCCCTG 
CTCTGCGCCC TGCGAGCCAA GGTGTGCGGG GCCTCCGGCC AGTTCGAGTT GGAGATCCTT 
TCCATGCAGA ACGTGAACGG GGAGCTGCAA AACGGGAACT GCTGTGGCGG CGCCCGGAAC 
CCGGGGGACC GCAAGTGCTC CCGCGACGAG TGTGACACGT ACTTCAAAGT GTGCCTCAAG 
GAGTACCAGT CGCGCGTCAC GGCCGGGGGG CCCTGCAGCT TTGGCTCGGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGTCGCG GCAACGAACG CAACCGCATC 
GTGCTGCCTT TCAGTTTTGC CTGGCCGAGG TCCTACACTT TGCTTGTGGA GGCGTGGGAT 
TCCAGCAATG ACACTGTCCA ACCCGACAGC ATTATTGAAA AGGCTTCTCA CTCAGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGGGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAT TACTATGGCT TCGGCTGCAA TAAGTTCTGC 
CGACCCAGAG ATGACTTCTT TGGCCACTAT GCCTGCGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC TGAATGTAAC AAAGCTATTT GCCGGCAGGG CTGTAGCCCC 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTATGGCTG GCAGGGCCTG 
TACTGTGACA AGTGCATCCC ACACCCAGGG TGCGTCCACG GCACCTGTAA TGAGCCCTGG 
CAGTGTCTCT GTGAGACCAA CTGGGGTGGC CAGCTCTGCG ACAAAGATCT CAACTACTGC 
GGGACCCACC AGCCATGTCT CAATGGGGGA ACTTGTAGCA ACACGGGGCC TGACAAATAT 
CAGTGTTCAT GCCCCGAGGG GTACTCAGGA CCCAACTGTG AAATTGCGGA ACACGCCTGC 
CTCTCTGATC CCTGCCACAA CAGAGGCAGC TGCAAGGAGA CCTCCCTGGG GTTTGAGTGT 
GAGTGTTCTC CGGGCTGGAC CGGCCCCACG TGCTCCACAA ACATTGATGA CTGTTCTCCA 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACTTGGTTA ACGGATTTAA GTGTGTGTGC 
CCGCCCCAGT GGACGGGCAA AACGTGCCAG TTAGATGCAA ATGAATGCGA GGCCAAACCT 
TGTGTAAATG CCAAATCCTG TAAGAATCTG ATTGCCAGCT ACTACTGTGA TTGCCTCCCG 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTCGGCCA ATGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGACAGA CATCGATGAG TGTGCCAGCA ACCCCTGTCT CAACGGGGGT 
CACTGTCAGA ACGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGCGAG CCCAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGCGACTA TTTCTGCAAG TGTCCCGAGG ACTATGAAGG CAAGAACTGC 
TCCCACCTTA AAGACCACTG CCGCACCACC CCCTGTGAAG TGATTGACAG CTGCACGGTG 
GCCATGGCCT CCAACGACAC GCCTGAAGGG GTGCGCTATA TTTCCTCCAA TGTCTGCGGT 
CCCCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACTGGAA CGTACTGCCA TGAAAATATT AACGACTGTG AGAGCAACCC CTGTAAAAAT 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGTA TCTGCAGTGG CGGCTGGGAA 
GGCGCCTACT GTGAGACCAA TATTAACGAC TGCAGCCAGA ACCCCTGCCA CAACGGGGGC 
TCGTGTCGCG ACCTGGTCAA CGACTTTTAC TGCGACTGTA AAAACGGGTG GAAAGGCAAG 
ACCTGCCACT CTCGTGACAG CCAGTGTGAC GAGGCTACGT GCAACAATGG TGGCACCTGC 
TATGACGAGG GGGACGCTTT CAAGTGCATG TGTCCCGGAG GCTGGGAAGG AACGACCTGT 
AACATAGCCC GCAACAGTAG CTGCCTGCCT AACCCCTGCC ACAATGGGGG CACCTGTGTG 
GTCAACGGCG AGTCCTTCAC GTGTGTCTGC AAAGAAGGCT GGGAGGGGCC CATCTGTACG 
CAGAATACCA ATGACTGCAG TCCTCATCCC TGTTACAACA GTGGCACGTG CGTGGATGGA 
GAGAACTGGT ACCGCTGTGA GTGCGCCCCC GGCTTCGCTG GGCCCGACTG CAGGATAAAC 
ATCAACGAAT GCCAGTCTTC ACCTTGTGCC TTCGGGGCAA CCTGTGTGGA TGAGATCAAT 
GGGTACCGGT GTGTCTGCCC CCCAGGGCAC AGTGGTGCTA AATGCCAGGA AGTTTCAGGG 
AGATCTTGCA TCACCGTGGG GAGCGTGATA CCTGATGGGG CCAAGTGGGA CGATGACTGC 
AACACCTGCC AGTGCCTGGA TGGGAGGATC GCCTGCTCGA AGGTGTGGTG TGGCCCCCGC 
CCTTGCTCGC TGCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGCCC AGTGCTTCGT GCGGCCCTGC ACTGGTGTGG GTGAGTGCCG GTCCTCCAGC 
CTCCAGCCCG TGAAGACCAA GTGTGCCTCC GACCCCTACT ACCAGGACAA CTGTGCCAAC 
ATCACGTTCA CCTTCAACAA GGAGATGATG TCGCCGGGTC TCACCACAGA GCATATTTGC 
AGCGAACTGC GGAATTTGAA TATTTTGAAG AATGTTTCTG CTGAATATTC AATCTACATA 
GCTTGCGAGC CTTCCCCTTC TGCAAACAAT GAAATACACG TGGCCATTTC TGCTGAAGAC 
ATCCGGGATG ATGGAAACCC AATCAAGGAA ATCACCGACA AAATTATAGA TCTTGTTAGT 
AAACGTGAAG GGAACAGCTC ACTGATCGCC GCTGTTGCGG AAGTGAGAGT TCAGAGGCGT 
CCTCTGAAGA ACAGAACAGA TTTCCTGGTG CCCCTGCTGA GCTCGGTCCT GACCGTGGCC 
TGGGTCTGCT GCCTGGTGAC CGCCTTCTAC TGGTGCGTGC GCAAGCGGCG GAAGCCCGGC 
AGCCACGCGC GCTCGGCCTC TGAGGAGAGC ACCACCAACA ACGTGCGGGA ACAGCTGAAC 
CAGATCAAGA ACCCCATCGA GAAGCACGGG GCCAACACAG TCCCCATCAA GGACTACGAG 
AACAAGAACT CCAAAATGTC CAAAATAAGG ACACACAACT CGGAGGTGGA AGAGGACGAC 
ATGGACAAGC ACCAGCAGAA AGCCCGCTTC GCCAGGCAGG CCACGTACAC GCTGGTCGAC 
AGAGAGGAGA AGTCCCCCCA CGGCACGCCG GCCAAACACC CGAACTGGAC AAACAAACAG 
GACAACAGAG ACCTGGAGAG CGCGCAGAGC CTGAACCGCA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023979157.1
CDS	499..4155
Translation	MRSPRTRGRPGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCSRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNERNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTCNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCETDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCKNGGTCIDGVNSYKCICSGGWEGAYCETNINDCSQNPCHNGGSCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICTQNTNDCSPHPCYNSGTCVDGENWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRSCITVGSVIPDGAKWDDDCNTCQCLDGRIACSKVWCGPRPCSLHKGHSECPSGQSCIPILDAQCFVRPCTGVGECRSSSLQPVKTKCASDPYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKREGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWVCCLVTAFYWCVRKRRKPGSHARSASEESTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFARQATYTLVDREEKSPHGTPAKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	XM_024123389.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024123389.1

ORF Insert Sequence:

ATGCGGTCCC CACGGACGCG CGGCCGGCCC GGGCGCCCCC TGAGCCTCCT GCTCGCCCTG 
CTCTGCGCCC TGCGAGCCAA GGTGTGCGGG GCCTCCGGCC AGTTCGAGTT GGAGATCCTT 
TCCATGCAGA ACGTGAACGG GGAGCTGCAA AACGGGAACT GCTGTGGCGG CGCCCGGAAC 
CCGGGGGACC GCAAGTGCTC CCGCGACGAG TGTGACACGT ACTTCAAAGT GTGCCTCAAG 
GAGTACCAGT CGCGCGTCAC GGCCGGGGGG CCCTGCAGCT TTGGCTCGGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGTCGCG GCAACGAACG CAACCGCATC 
GTGCTGCCTT TCAGTTTTGC CTGGCCGAGG TCCTACACTT TGCTTGTGGA GGCGTGGGAT 
TCCAGCAATG ACACTGTCCA ACCCGACAGC ATTATTGAAA AGGCTTCTCA CTCAGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGGGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAT TACTATGGCT TCGGCTGCAA TAAGTTCTGC 
CGACCCAGAG ATGACTTCTT TGGCCACTAT GCCTGCGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC TGAATGTAAC AAAGCTATTT GCCGGCAGGG CTGTAGCCCC 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTATGGCTG GCAGGGCCTG 
TACTGTGACA AGTGCATCCC ACACCCAGGG TGCGTCCACG GCACCTGTAA TGAGCCCTGG 
CAGTGTCTCT GTGAGACCAA CTGGGGTGGC CAGCTCTGCG ACAAAGATCT CAACTACTGC 
GGGACCCACC AGCCATGTCT CAATGGGGGA ACTTGTAGCA ACACGGGGCC TGACAAATAT 
CAGTGTTCAT GCCCCGAGGG GTACTCAGGA CCCAACTGTG AAATTGCGGA ACACGCCTGC 
CTCTCTGATC CCTGCCACAA CAGAGGCAGC TGCAAGGAGA CCTCCCTGGG GTTTGAGTGT 
GAGTGTTCTC CGGGCTGGAC CGGCCCCACG TGCTCCACAA ACATTGATGA CTGTTCTCCA 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACTTGGTTA ACGGATTTAA GTGTGTGTGC 
CCGCCCCAGT GGACGGGCAA AACGTGCCAG TTAGATGCAA ATGAATGCGA GGCCAAACCT 
TGTGTAAATG CCAAATCCTG TAAGAATCTG ATTGCCAGCT ACTACTGTGA TTGCCTCCCG 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTCGGCCA ATGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGACAGA CATCGATGAG TGTGCCAGCA ACCCCTGTCT CAACGGGGGT 
CACTGTCAGA ACGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGCGAG CCCAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGCGACTA TTTCTGCAAG TGTCCCGAGG ACTATGAAGG CAAGAACTGC 
TCCCACCTTA AAGACCACTG CCGCACCACC CCCTGTGAAG TGATTGACAG CTGCACGGTG 
GCCATGGCCT CCAACGACAC GCCTGAAGGG GTGCGCTATA TTTCCTCCAA TGTCTGCGGT 
CCCCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACTGGAA CGTACTGCCA TGAAAATATT AACGACTGTG AGAGCAACCC CTGTAAAAAT 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGTA TCTGCAGTGG CGGCTGGGAA 
GGCGCCTACT GTGAGACCAA TATTAACGAC TGCAGCCAGA ACCCCTGCCA CAACGGGGGC 
TCGTGTCGCG ACCTGGTCAA CGACTTTTAC TGCGACTGTA AAAACGGGTG GAAAGGCAAG 
ACCTGCCACT CTCGTGACAG CCAGTGTGAC GAGGCTACGT GCAACAATGG TGGCACCTGC 
TATGACGAGG GGGACGCTTT CAAGTGCATG TGTCCCGGAG GCTGGGAAGG AACGACCTGT 
AACATAGCCC GCAACAGTAG CTGCCTGCCT AACCCCTGCC ACAATGGGGG CACCTGTGTG 
GTCAACGGCG AGTCCTTCAC GTGTGTCTGC AAAGAAGGCT GGGAGGGGCC CATCTGTACG 
CAGAATACCA ATGACTGCAG TCCTCATCCC TGTTACAACA GTGGCACGTG CGTGGATGGA 
GAGAACTGGT ACCGCTGTGA GTGCGCCCCC GGCTTCGCTG GGCCCGACTG CAGGATAAAC 
ATCAACGAAT GCCAGTCTTC ACCTTGTGCC TTCGGGGCAA CCTGTGTGGA TGAGATCAAT 
GGGTACCGGT GTGTCTGCCC CCCAGGGCAC AGTGGTGCTA AATGCCAGGA AGTTTCAGGG 
AGATCTTGCA TCACCGTGGG GAGCGTGATA CCTGATGGGG CCAAGTGGGA CGATGACTGC 
AACACCTGCC AGTGCCTGGA TGGGAGGATC GCCTGCTCGA AGGTGTGGTG TGGCCCCCGC 
CCTTGCTCGC TGCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGCCC AGTGCTTCGT GCGGCCCTGC ACTGGTGTGG GTGAGTGCCG GTCCTCCAGC 
CTCCAGCCCG TGAAGACCAA GTGTGCCTCC GACCCCTACT ACCAGGACAA CTGTGCCAAC 
ATCACGTTCA CCTTCAACAA GGAGATGATG TCGCCGGGTC TCACCACAGA GCATATTTGC 
AGCGAACTGC GGAATTTGAA TATTTTGAAG AATGTTTCTG CTGAATATTC AATCTACATA 
GCTTGCGAGC CTTCCCCTTC TGCAAACAAT GAAATACACG TGGCCATTTC TGCTGAAGAC 
ATCCGGGATG ATGGAAACCC AATCAAGGAA ATCACCGACA AAATTATAGA TCTTGTTAGT 
AAACGTGAAG GGAACAGCTC ACTGATCGCC GCTGTTGCGG AAGTGAGAGT TCAGAGGCGT 
CCTCTGAAGA ACAGAACAGA TTTCCTGGTG CCCCTGCTGA GCTCGGTCCT GACCGTGGCC 
TGGGTCTGCT GCCTGGTGAC CGCCTTCTAC TGGTGCGTGC GCAAGCGGCG GAAGCCCGGC 
AGCCACGCGC GCTCGGCCTC TGAGGAGAGC ACCACCAACA ACGTGCGGGA ACAGCTGAAC 
CAGATCAAGA ACCCCATCGA GAAGCACGGG GCCAACACAG TCCCCATCAA GGACTACGAG 
AACAAGAACT CCAAAATGTC CAAAATAAGG ACACACAACT CGGAGGTGGA AGAGGACGAC 
ATGGACAAGC ACCAGCAGAA AGCCCGCTTC GCCAGGCAGG CCACGTACAC GCTGGTCGAC 
AGAGAGGAGA AGTCCCCCCA CGGCACGCCG GCCAAACACC CGAACTGGAC AAACAAACAG 
GACAACAGAG ACCTGGAGAG CGCGCAGAGC CTGAACCGCA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAG1 ( XM_028498654.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028354455.1 Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X2, mRNA.

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CloneID	OPh321162
Clone ID Related Accession (Same CDS sequence)	XM_028498654.1
Accession Version	XM_028498654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	protein jagged-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTCCC CACGGACGCG CGGCCGGCCC GGGCGCCCCC TGAGCCTCCT GCTCGCCCTG 
CTCTGCGCCC TGCGAGCCAA GGTGTGCGGG GCCTCCGGCC AGTTCGAGTT GGAGATCCTT 
TCCATGCAGA ACGTGAACGG GGAGCTGCAA AACGGGAACT GCTGTGGCGG CGCCCGGAAC 
CCGGGGGACC GCAAGTGCTC CCGCGACGAG TGTGACACGT ACTTCAAAGT GTGCCTCAAG 
GAGTACCAGT CGCGCGTCAC GGCCGGGGGG CCCTGCAGCT TTGGCTCGGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGTCGCG GCAACGAACG CAACCGCATC 
GTGCTGCCTT TCAGTTTTGC CTGGCCGAGG TCCTACACTT TGCTTGTGGA GGCGTGGGAT 
TCCAGCAATG ACACTGTCCA ACCCGACAGC ATTATTGAAA AGGCTTCTCA CTCAGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGGGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAT TACTATGGCT TCGGCTGCAA TAAGTTCTGC 
CGACCCAGAG ATGACTTCTT TGGCCACTAT GCCTGCGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC TGAATGTAAC AAAGCTATTT GCCGGCAGGG CTGTAGCCCC 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTATGGCTG GCAGGGCCTG 
TACTGTGACA AGTGCATCCC ACACCCAGGG TGCGTCCACG GCACCTGTAA TGAGCCCTGG 
CAGTGTCTCT GTGAGACCAA CTGGGGTGGC CAGCTCTGCG ACAAAGATCT CAACTACTGC 
GGGACCCACC AGCCATGTCT CAATGGGGGA ACTTGTAGCA ACACGGGGCC TGACAAATAT 
CAGTGTTCAT GCCCCGAGGG GTACTCAGGA CCCAACTGTG AAATTGCGGA ACACGCCTGC 
CTCTCTGATC CCTGCCACAA CAGAGGCAGC TGCAAGGAGA CCTCCCTGGG GTTTGAGTGT 
GAGTGTTCTC CGGGCTGGAC CGGCCCCACG TGCTCCACAA ATGCAAATGA ATGCGAGGCC 
AAACCTTGTG TAAATGCCAA ATCCTGTAAG AATCTGATTG CCAGCTACTA CTGTGATTGC 
CTCCCGGGCT GGATGGGTCA GAATTGTGAC ATAAATATTA ATGACTGCCT CGGCCAATGT 
CAGAATGACG CCTCCTGTCG GGATTTGGTT AATGGTTATC GCTGTATCTG TCCACCTGGC 
TATGCAGGCG ATCACTGTGA GACAGACATC GATGAGTGTG CCAGCAACCC CTGTCTCAAC 
GGGGGTCACT GTCAGAACGA AATCAACAGA TTCCAGTGTC TGTGTCCCAC TGGTTTCTCT 
GGAAACCTCT GTCAGCTGGA CATCGATTAT TGCGAGCCCA ATCCCTGCCA GAACGGTGCC 
CAGTGCTACA ACCGTGCCAG CGACTATTTC TGCAAGTGTC CCGAGGACTA TGAAGGCAAG 
AACTGCTCCC ACCTTAAAGA CCACTGCCGC ACCACCCCCT GTGAAGTGAT TGACAGCTGC 
ACGGTGGCCA TGGCCTCCAA CGACACGCCT GAAGGGGTGC GCTATATTTC CTCCAATGTC 
TGCGGTCCCC ACGGGAAGTG CAAGAGTCAG TCGGGAGGCA AATTCACCTG TGACTGTAAC 
AAAGGCTTCA CTGGAACGTA CTGCCATGAA AATATTAACG ACTGTGAGAG CAACCCCTGT 
AAAAATGGTG GCACTTGCAT CGATGGTGTC AACTCCTACA AGTGTATCTG CAGTGGCGGC 
TGGGAAGGCG CCTACTGTGA GACCAATATT AACGACTGCA GCCAGAACCC CTGCCACAAC 
GGGGGCTCGT GTCGCGACCT GGTCAACGAC TTTTACTGCG ACTGTAAAAA CGGGTGGAAA 
GGCAAGACCT GCCACTCTCG TGACAGCCAG TGTGACGAGG CTACGTGCAA CAATGGTGGC 
ACCTGCTATG ACGAGGGGGA CGCTTTCAAG TGCATGTGTC CCGGAGGCTG GGAAGGAACG 
ACCTGTAACA TAGCCCGCAA CAGTAGCTGC CTGCCTAACC CCTGCCACAA TGGGGGCACC 
TGTGTGGTCA ACGGCGAGTC CTTCACGTGT GTCTGCAAAG AAGGCTGGGA GGGGCCCATC 
TGTACGCAGA ATACCAATGA CTGCAGTCCT CATCCCTGTT ACAACAGTGG CACGTGCGTG 
GATGGAGAGA ACTGGTACCG CTGTGAGTGC GCCCCCGGCT TCGCTGGGCC CGACTGCAGG 
ATAAACATCA ACGAATGCCA GTCTTCACCT TGTGCCTTCG GGGCAACCTG TGTGGATGAG 
ATCAATGGGT ACCGGTGTGT CTGCCCCCCA GGGCACAGTG GTGCTAAATG CCAGGAAGTT 
TCAGGGAGAT CTTGCATCAC CGTGGGGAGC GTGATACCTG ATGGGGCCAA GTGGGACGAT 
GACTGCAACA CCTGCCAGTG CCTGGATGGG AGGATCGCCT GCTCGAAGGT GTGGTGTGGC 
CCCCGCCCTT GCTCGCTGCA CAAAGGGCAC AGCGAGTGCC CCAGCGGGCA GAGCTGCATC 
CCCATCCTGG ACGCCCAGTG CTTCGTGCGG CCCTGCACTG GTGTGGGTGA GTGCCGGTCC 
TCCAGCCTCC AGCCCGTGAA GACCAAGTGT GCCTCCGACC CCTACTACCA GGACAACTGT 
GCCAACATCA CGTTCACCTT CAACAAGGAG ATGATGTCGC CGGGTCTCAC CACAGAGCAT 
ATTTGCAGCG AACTGCGGAA TTTGAATATT TTGAAGAATG TTTCTGCTGA ATATTCAATC 
TACATAGCTT GCGAGCCTTC CCCTTCTGCA AACAATGAAA TACACGTGGC CATTTCTGCT 
GAAGACATCC GGGATGATGG AAACCCAATC AAGGAAATCA CCGACAAAAT TATAGATCTT 
GTTAGTAAAC GTGAAGGGAA CAGCTCACTG ATCGCCGCTG TTGCGGAAGT GAGAGTTCAG 
AGGCGTCCTC TGAAGAACAG AACAGATTTC CTGGTGCCCC TGCTGAGCTC GGTCCTGACC 
GTGGCCTGGG TCTGCTGCCT GGTGACCGCC TTCTACTGGT GCGTGCGCAA GCGGCGGAAG 
CCCGGCAGCC ACGCGCGCTC GGCCTCTGAG GAGAGCACCA CCAACAACGT GCGGGAACAG 
CTGAACCAGA TCAAGAACCC CATCGAGAAG CACGGGGCCA ACACAGTCCC CATCAAGGAC 
TACGAGAACA AGAACTCCAA AATGTCCAAA ATAAGGACAC ACAACTCGGA GGTGGAAGAG 
GACGACATGG ACAAGCACCA GCAGAAAGCC CGCTTCGCCA GGCAGGCCAC GTACACGCTG 
GTCGACAGAG AGGAGAAGTC CCCCCACGGC ACGCCGGCCA AACACCCGAA CTGGACAAAC 
AAACAGGACA ACAGAGACCT GGAGAGCGCG CAGAGCCTGA ACCGCATGGA GTACATCGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028354455.1
CDS	22..3564
Translation	MRSPRTRGRPGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCSRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNERNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTCNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCETDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCKNGGTCIDGVNSYKCICSGGWEGAYCETNINDCSQNPCHNGGSCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICTQNTNDCSPHPCYNSGTCVDGENWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRSCITVGSVIPDGAKWDDDCNTCQCLDGRIACSKVWCGPRPCSLHKGHSECPSGQSCIPILDAQCFVRPCTGVGECRSSSLQPVKTKCASDPYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKREGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWVCCLVTAFYWCVRKRRKPGSHARSASEESTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFARQATYTLVDREEKSPHGTPAKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	XM_028498654.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon jagged canonical Notch ligand 1 (JAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028498654.1

ORF Insert Sequence:

ATGCGGTCCC CACGGACGCG CGGCCGGCCC GGGCGCCCCC TGAGCCTCCT GCTCGCCCTG 
CTCTGCGCCC TGCGAGCCAA GGTGTGCGGG GCCTCCGGCC AGTTCGAGTT GGAGATCCTT 
TCCATGCAGA ACGTGAACGG GGAGCTGCAA AACGGGAACT GCTGTGGCGG CGCCCGGAAC 
CCGGGGGACC GCAAGTGCTC CCGCGACGAG TGTGACACGT ACTTCAAAGT GTGCCTCAAG 
GAGTACCAGT CGCGCGTCAC GGCCGGGGGG CCCTGCAGCT TTGGCTCGGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGTCGCG GCAACGAACG CAACCGCATC 
GTGCTGCCTT TCAGTTTTGC CTGGCCGAGG TCCTACACTT TGCTTGTGGA GGCGTGGGAT 
TCCAGCAATG ACACTGTCCA ACCCGACAGC ATTATTGAAA AGGCTTCTCA CTCAGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGGGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAT TACTATGGCT TCGGCTGCAA TAAGTTCTGC 
CGACCCAGAG ATGACTTCTT TGGCCACTAT GCCTGCGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC TGAATGTAAC AAAGCTATTT GCCGGCAGGG CTGTAGCCCC 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTATGGCTG GCAGGGCCTG 
TACTGTGACA AGTGCATCCC ACACCCAGGG TGCGTCCACG GCACCTGTAA TGAGCCCTGG 
CAGTGTCTCT GTGAGACCAA CTGGGGTGGC CAGCTCTGCG ACAAAGATCT CAACTACTGC 
GGGACCCACC AGCCATGTCT CAATGGGGGA ACTTGTAGCA ACACGGGGCC TGACAAATAT 
CAGTGTTCAT GCCCCGAGGG GTACTCAGGA CCCAACTGTG AAATTGCGGA ACACGCCTGC 
CTCTCTGATC CCTGCCACAA CAGAGGCAGC TGCAAGGAGA CCTCCCTGGG GTTTGAGTGT 
GAGTGTTCTC CGGGCTGGAC CGGCCCCACG TGCTCCACAA ATGCAAATGA ATGCGAGGCC 
AAACCTTGTG TAAATGCCAA ATCCTGTAAG AATCTGATTG CCAGCTACTA CTGTGATTGC 
CTCCCGGGCT GGATGGGTCA GAATTGTGAC ATAAATATTA ATGACTGCCT CGGCCAATGT 
CAGAATGACG CCTCCTGTCG GGATTTGGTT AATGGTTATC GCTGTATCTG TCCACCTGGC 
TATGCAGGCG ATCACTGTGA GACAGACATC GATGAGTGTG CCAGCAACCC CTGTCTCAAC 
GGGGGTCACT GTCAGAACGA AATCAACAGA TTCCAGTGTC TGTGTCCCAC TGGTTTCTCT 
GGAAACCTCT GTCAGCTGGA CATCGATTAT TGCGAGCCCA ATCCCTGCCA GAACGGTGCC 
CAGTGCTACA ACCGTGCCAG CGACTATTTC TGCAAGTGTC CCGAGGACTA TGAAGGCAAG 
AACTGCTCCC ACCTTAAAGA CCACTGCCGC ACCACCCCCT GTGAAGTGAT TGACAGCTGC 
ACGGTGGCCA TGGCCTCCAA CGACACGCCT GAAGGGGTGC GCTATATTTC CTCCAATGTC 
TGCGGTCCCC ACGGGAAGTG CAAGAGTCAG TCGGGAGGCA AATTCACCTG TGACTGTAAC 
AAAGGCTTCA CTGGAACGTA CTGCCATGAA AATATTAACG ACTGTGAGAG CAACCCCTGT 
AAAAATGGTG GCACTTGCAT CGATGGTGTC AACTCCTACA AGTGTATCTG CAGTGGCGGC 
TGGGAAGGCG CCTACTGTGA GACCAATATT AACGACTGCA GCCAGAACCC CTGCCACAAC 
GGGGGCTCGT GTCGCGACCT GGTCAACGAC TTTTACTGCG ACTGTAAAAA CGGGTGGAAA 
GGCAAGACCT GCCACTCTCG TGACAGCCAG TGTGACGAGG CTACGTGCAA CAATGGTGGC 
ACCTGCTATG ACGAGGGGGA CGCTTTCAAG TGCATGTGTC CCGGAGGCTG GGAAGGAACG 
ACCTGTAACA TAGCCCGCAA CAGTAGCTGC CTGCCTAACC CCTGCCACAA TGGGGGCACC 
TGTGTGGTCA ACGGCGAGTC CTTCACGTGT GTCTGCAAAG AAGGCTGGGA GGGGCCCATC 
TGTACGCAGA ATACCAATGA CTGCAGTCCT CATCCCTGTT ACAACAGTGG CACGTGCGTG 
GATGGAGAGA ACTGGTACCG CTGTGAGTGC GCCCCCGGCT TCGCTGGGCC CGACTGCAGG 
ATAAACATCA ACGAATGCCA GTCTTCACCT TGTGCCTTCG GGGCAACCTG TGTGGATGAG 
ATCAATGGGT ACCGGTGTGT CTGCCCCCCA GGGCACAGTG GTGCTAAATG CCAGGAAGTT 
TCAGGGAGAT CTTGCATCAC CGTGGGGAGC GTGATACCTG ATGGGGCCAA GTGGGACGAT 
GACTGCAACA CCTGCCAGTG CCTGGATGGG AGGATCGCCT GCTCGAAGGT GTGGTGTGGC 
CCCCGCCCTT GCTCGCTGCA CAAAGGGCAC AGCGAGTGCC CCAGCGGGCA GAGCTGCATC 
CCCATCCTGG ACGCCCAGTG CTTCGTGCGG CCCTGCACTG GTGTGGGTGA GTGCCGGTCC 
TCCAGCCTCC AGCCCGTGAA GACCAAGTGT GCCTCCGACC CCTACTACCA GGACAACTGT 
GCCAACATCA CGTTCACCTT CAACAAGGAG ATGATGTCGC CGGGTCTCAC CACAGAGCAT 
ATTTGCAGCG AACTGCGGAA TTTGAATATT TTGAAGAATG TTTCTGCTGA ATATTCAATC 
TACATAGCTT GCGAGCCTTC CCCTTCTGCA AACAATGAAA TACACGTGGC CATTTCTGCT 
GAAGACATCC GGGATGATGG AAACCCAATC AAGGAAATCA CCGACAAAAT TATAGATCTT 
GTTAGTAAAC GTGAAGGGAA CAGCTCACTG ATCGCCGCTG TTGCGGAAGT GAGAGTTCAG 
AGGCGTCCTC TGAAGAACAG AACAGATTTC CTGGTGCCCC TGCTGAGCTC GGTCCTGACC 
GTGGCCTGGG TCTGCTGCCT GGTGACCGCC TTCTACTGGT GCGTGCGCAA GCGGCGGAAG 
CCCGGCAGCC ACGCGCGCTC GGCCTCTGAG GAGAGCACCA CCAACAACGT GCGGGAACAG 
CTGAACCAGA TCAAGAACCC CATCGAGAAG CACGGGGCCA ACACAGTCCC CATCAAGGAC 
TACGAGAACA AGAACTCCAA AATGTCCAAA ATAAGGACAC ACAACTCGGA GGTGGAAGAG 
GACGACATGG ACAAGCACCA GCAGAAAGCC CGCTTCGCCA GGCAGGCCAC GTACACGCTG 
GTCGACAGAG AGGAGAAGTC CCCCCACGGC ACGCCGGCCA AACACCCGAA CTGGACAAAC 
AAACAGGACA ACAGAGACCT GGAGAGCGCG CAGAGCCTGA ACCGCATGGA GTACATCGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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JAG1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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