WRN cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	WRN
Entrez Gene ID	472732
Full Name	Werner syndrome RecQ like helicase
General protein information	Preferred Names Werner syndrome RecQ like helicase Names Werner syndrome ATP-dependent helicase Werner syndrome protein Werner syndrome, RecQ helicase-like
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_528104.7	XP_528104.3	Werner syndrome ATP-dependent helicase isoform X1
XM_528104.6	XP_528104.3	Werner syndrome ATP-dependent helicase isoform X1
XM_009455155.2	XP_009453430.2	Werner syndrome ATP-dependent helicase isoform X2
XM_016959317.1	XP_016814806.1	Werner syndrome ATP-dependent helicase isoform X3
XM_016959317.2	XP_016814806.1	Werner syndrome ATP-dependent helicase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
Macaca mulatta (Rhesus monkey)	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Oryza sativa (rice)	Os07g0681600	NP_001060655.1
Danio rerio (zebrafish)	wrn	XP_697980.5

Gene Ontology

The following WRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc108645	XM_528104.7 Latest version!	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc87748	XM_528104.6 Latest version!	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc87749	XM_009455155.2 Latest version!	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc87750	XM_016959317.2 Latest version!	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OPc87750	XM_016959317.1	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WRN ( XM_528104.7 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_528104.3 Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc108645
Clone ID Related Accession (Same CDS sequence)	XM_528104.7
Accession Version	XM_528104.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_528104.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATAATGA TGAAAACGAT 
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA 
AACCTCAACA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT 
CTGGGTCTTC TTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA 
GAAGATGATG ATAAGGACTT TTTGTTGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC 
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA 
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC 
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT 
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA 
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC 
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT 
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT 
AGGAATTCAT TCAGGAAGTT GGGCTCCCTG AAGACAGCAC TGCCAATGGT TCCAATCGTT 
GCACTTACTG CTACTGCAAG TTCCTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG 
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG 
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC 
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT 
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT 
AACACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT 
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT 
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT 
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT 
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGACGGC AAAGATGGAA 
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA 
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG 
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT 
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG 
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG 
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT 
CAGCTGATCA CTGAGGGATT CTTGGAAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT 
TGCATCCTTA CGAAAGAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC 
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA 
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAACTACA 
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT 
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC 
TCAGAGCCTA TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG 
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACC 
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG 
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA 
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA 
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC 
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG 
ATTCTGCCTC TTATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC 
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT 
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC 
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG 
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT 
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA 
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_528104.3
CDS	789..5087
Translation	MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVWKSVFEDDLPFLEFTGSIVYSYDASDCSLLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHISSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFGDSTTGGVQQKQIREHEVLIHVADETWDPTLDHLAKHDGEGVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQAQEEYLSDTAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNNDENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLLTKEEEEDDENEANEGEEDDDKDFLLPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRNSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFNTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMTAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLEEVSRYNKFMKICILTKEGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQVPVELTTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSEPIISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPSSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

Target ORF information:

RefSeq Version	XM_528104.7
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_528104.7

ORF Insert Sequence:

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATAATGA TGAAAACGAT 
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA 
AACCTCAACA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT 
CTGGGTCTTC TTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA 
GAAGATGATG ATAAGGACTT TTTGTTGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC 
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA 
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC 
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT 
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA 
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC 
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT 
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT 
AGGAATTCAT TCAGGAAGTT GGGCTCCCTG AAGACAGCAC TGCCAATGGT TCCAATCGTT 
GCACTTACTG CTACTGCAAG TTCCTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG 
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG 
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC 
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT 
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT 
AACACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT 
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT 
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT 
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT 
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGACGGC AAAGATGGAA 
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA 
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG 
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT 
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG 
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG 
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT 
CAGCTGATCA CTGAGGGATT CTTGGAAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT 
TGCATCCTTA CGAAAGAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC 
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA 
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAACTACA 
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT 
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC 
TCAGAGCCTA TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG 
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACC 
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG 
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA 
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA 
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC 
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG 
ATTCTGCCTC TTATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC 
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT 
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC 
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG 
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT 
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA 
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_528104.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_528104.3 Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc87748
Clone ID Related Accession (Same CDS sequence)	XM_528104.6
Accession Version	XM_528104.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006475.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_528104.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA TGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATAATGA TGAAAACGAT 
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA 
AACCTCAACA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT 
CTGGGTCTTC TTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA 
GAAGATGATG ATAAGGACTT TTTGTTGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC 
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA 
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC 
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT 
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA 
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC 
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT 
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT 
AGGAATTCAT TCAGGAAGTT GGGCTCCCTG AAGACAGCAC TGCCAATGGT TCCAATCGTT 
GCACTTACTG CTACTGCAAG TTCCTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG 
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG 
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC 
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT 
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT 
AACACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT 
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT 
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT 
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT 
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGACGGC AAAGATGGAA 
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA 
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG 
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT 
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG 
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG 
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT 
CAGCTGATCA CTGAGGGATT CTTGGAAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT 
TGCATCCTTA CGAAAGAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC 
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA 
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAACTACA 
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT 
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC 
TCAGAGCCTA TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG 
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACC 
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG 
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA 
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA 
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC 
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG 
ATTCTGCCTC TTATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC 
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT 
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC 
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG 
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT 
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA 
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_528104.3
CDS	784..5082
Translation	MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVWKSVFEDDLPFLEFTGSIVYSYDASDCSLLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHISSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFGDSTTGGVQQKQIREHEVLIHVADETWDPTLDHLAKHDGEGVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQAQEEYLSDTAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNNDENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLLTKEEEEDDENEANEGEEDDDKDFLLPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRNSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFNTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMTAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLEEVSRYNKFMKICILTKEGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQVPVELTTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSEPIISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPSSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

Target ORF information:

RefSeq Version	XM_528104.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_528104.6

ORF Insert Sequence:

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA TGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATAATGA TGAAAACGAT 
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA 
AACCTCAACA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT 
CTGGGTCTTC TTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA 
GAAGATGATG ATAAGGACTT TTTGTTGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC 
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA 
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC 
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT 
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA 
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC 
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT 
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT 
AGGAATTCAT TCAGGAAGTT GGGCTCCCTG AAGACAGCAC TGCCAATGGT TCCAATCGTT 
GCACTTACTG CTACTGCAAG TTCCTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG 
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG 
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC 
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT 
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT 
AACACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT 
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT 
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT 
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT 
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGACGGC AAAGATGGAA 
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA 
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG 
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT 
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG 
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG 
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT 
CAGCTGATCA CTGAGGGATT CTTGGAAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT 
TGCATCCTTA CGAAAGAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC 
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA 
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAACTACA 
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT 
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC 
TCAGAGCCTA TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG 
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACC 
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG 
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA 
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA 
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC 
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG 
ATTCTGCCTC TTATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC 
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT 
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC 
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG 
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT 
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA 
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_009455155.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009453430.2 Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

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CloneID	OPc87749
Clone ID Related Accession (Same CDS sequence)	XM_009455155.2
Accession Version	XM_009455155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006475.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009455155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA TGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTCAACAG TGGCACGGTA 
GAACCAACTC ATTCTAAATG CTTAAAAATG GAAAGAAATC TGGGTCTTCT TACTAAAGAA 
GAAGAAGAAG ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT 
TTGTTGCCAG CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC 
AGTTTTAAAC CAGTTCAGTG GAAAGTGATT CATTCAGTAT TAGAAGAAAG AAGAGATAAT 
GTTGCTGTCA TGGCAACTGG ATATGGAAAG AGTTTGTGCT TCCAGTATCC ACCTGTTTAT 
GTAGGCAAGA TTGGCCTTGT TATCTCTCCC CTTATTTCTC TGATGGAAGA CCAAGTGCTA 
CAGCTTAAAA TGTCCAACAT CCCAGCTTGC TTCCTTGGAT CAGCACAGTC AGAAAATGTT 
CTAACAGATA TTAAATTAGG TAAATACCGG ATTGTATACG TAACTCCAGA ATACTGTTCA 
GGTAACATGG GCCTGCTCCA GCAACTTGAG GCTGATATTG GTATCACGCT CATTGCTGTG 
GATGAGGCTC ACTGTATTTC TGAGTGGGGG CATGATTTTA GGAATTCATT CAGGAAGTTG 
GGCTCCCTGA AGACAGCACT GCCAATGGTT CCAATCGTTG CACTTACTGC TACTGCAAGT 
TCCTCAATCC GGGAAGACAT TGTACGTTGC TTAAATCTGA GAAATCCTCA GATCACCTGT 
ACTGGTTTTG ATCGACCAAA CCTGTATTTA GAAGTTAGGC GAAAAACAGG GAATATCCTT 
CAGGATCTGC AGCCATTTCT TGTCAAAACA AGTTCCCACT GGGAATTTGA AGGTCCAACA 
ATCATCTACT GTCCTTCTAG AAAAATGACA CAACAAGTTA CAGGTGAACT TAGGAAACTG 
AATCTATCCT GTGGAACATA CCATGCGGGC ATGAGTTTTA ACACAAGGAA AGACATTCAT 
CATAGGTTTG TAAGAGATGA AATTCAGTGT GTCATAGCTA CCATAGCTTT TGGAATGGGC 
ATTAATAAAG CTGACATTCG CCAAGTCATT CATTACGGTG CTCCTAAGGA CATGGAATCA 
TATTATCAGG AGATTGGTAG AGCTGGTCGT GATGGACTTC AAAGTTCTTG TCACGTCCTC 
TGGGCTCCTG CAGACATTAA CTTAAATAGG CACCTTCTTA CTGAGATACG TAATGAGAAG 
TTTCGATTAT ACAAATTAAA GATGACGGCA AAGATGGAAA AATATCTTCA TTCTAGCAGA 
TGTAGGAGAC AAATCATCTT GTCTCATTTT GAGGACAAAC AAGTACAAAA AGCCTCCTTG 
GGAATTATGG GAACTGAAAA ATGCTGTGAT AATTGCAGGT CCAGATTGGA TCATTGCTAT 
TCCATGGATG ACTCAGAGGA TACATCCTGG GACTTTGGTC CACAAGCATT TAAGCTTTTG 
TCTGCTGTGG ACATCTTAGG CGAAAAATTT GGAATTGGGC TTCCAATTTT ATTTCTCCGA 
GGATCTAATT CTCAGCGTCT TGCCGATCAA TATCGCAGGC ACAGTTTATT TGGCACTGGC 
AAGGATCAAA CAGAGAGTTG GTGGAAGGCT TTTTCCCGTC AGCTGATCAC TGAGGGATTC 
TTGGAAGAAG TTTCTCGGTA TAACAAATTT ATGAAGATTT GCATCCTTAC GAAAGAGGGT 
AGAAATTGGC TTCATAAAGC TAATACAGAA TCTCAGAGCC TCATCCTTCA AGCTAATGAA 
GAATTGTGTC CAAAGAAGTT TCTTCTGCCT AGTTCAAAAA CTGTATCTTC GGGCACCAAA 
GAGCATTGTT ATAATCAAGT ACCAGTTGAA TTAACTACAG AGAAGAAGTC TAACTTGGAG 
AAGTTATATT CTTATAAACC ATGTGATAAG ATTTCTTCTG GGAGTAACAT TTCTAAAAAA 
AGTATCATGG TACAGTCACC AGAAAAAGCT TACAGTTCCT CAGAGCCTAT TATTTCGGCA 
CAAGAGCAGG AGACTCAGAT TGTGTTATAT GGCAAATTGG TAGAAGCTAG GCAGAAACAT 
GCCAATAAAA TGGATGTTCC CCCAGCTATT CTGGCAACCA ACAAGATACT GGTGGATATG 
GCCAAAATGA GACCAACTAC GGTTGAAAAC GTAAAAAGGA TTGATGGTGT TTCTGAAGGC 
AAAGCTGCCA TGTTGGCCCC TCTGTTGGAA GTCATCAAAC ATTTCTGCCA AACAAATAGT 
GTTCAGACAG ACCTCTTTTC AAGTACAAAA CCTCAAGAAG AACAGAAGAC GAGTCTGGTA 
GCAAAAAATA AAATATGCAC ACTTTCACAG TCTATGGCCA TCACATACTC TTTATTCCAA 
GAAAAGAAGA TGCCTTTGAA GAGCATAGCT GAGAGCAGGA TTCTGCCTCT TATGACAATT 
GGCATGCACT TATCCCAAGC GGTGAAAGCT GGCTGCCCCC TTGATTTGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTGCT GATGTTATCC GAAACCCTCC CGTCAACTCA 
GATATGAGTA AAATTAGCCT AATCAGAATG TTAGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG CAATTGAGAT CCTTAAACAT GGTCCTGACA GCGGACTTCA ACCTTCATGT 
GATGTCAACA AAAGGAGATG TTTTCCCAGT TCTGAAGAGA TCTGTTCAAG TTCTAAGAGA 
AGCAAGGAAG AAGTAGGCAT CAATACTGAG ACTTCATCTG CAGAGAGAAA GAGACGATTA 
CCTGTGTGGT TTGCCAAAGG AAGTGATACC AGCAAGAAAT TAATGGACAA AACGAAAAGG 
GGAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009453430.2
CDS	259..4476
Translation	MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVWKSVFEDDLPFLEFTGSIVYSYDASDCSLLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHISSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFGDSTTGGVQQKQIREHEVLIHVADETWDPTLDHLAKHDGEGVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQAQEEYLSDTAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLLTKEEEEDDENEANEGEEDDDKDFLLPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRNSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFNTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMTAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLEEVSRYNKFMKICILTKEGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQVPVELTTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSEPIISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPSSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

Target ORF information:

RefSeq Version	XM_009455155.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009455155.2

ORF Insert Sequence:

ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTTGGAA GAGTGTTTTT 
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA TGATGCTAGT 
GATTGCTCTT TGCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA 
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT 
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACA TTTCTTCCAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG 
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGTACAGAGA CCTGGAGCCT CAACAGTCTG 
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT 
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA 
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AACTGGAAAA CCCACGGAGG 
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT 
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT 
GGAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT 
CACGTTGCAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC 
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAGAATATC TTAGTGATAC TGCTTATAAA 
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTCAACAG TGGCACGGTA 
GAACCAACTC ATTCTAAATG CTTAAAAATG GAAAGAAATC TGGGTCTTCT TACTAAAGAA 
GAAGAAGAAG ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT 
TTGTTGCCAG CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC 
AGTTTTAAAC CAGTTCAGTG GAAAGTGATT CATTCAGTAT TAGAAGAAAG AAGAGATAAT 
GTTGCTGTCA TGGCAACTGG ATATGGAAAG AGTTTGTGCT TCCAGTATCC ACCTGTTTAT 
GTAGGCAAGA TTGGCCTTGT TATCTCTCCC CTTATTTCTC TGATGGAAGA CCAAGTGCTA 
CAGCTTAAAA TGTCCAACAT CCCAGCTTGC TTCCTTGGAT CAGCACAGTC AGAAAATGTT 
CTAACAGATA TTAAATTAGG TAAATACCGG ATTGTATACG TAACTCCAGA ATACTGTTCA 
GGTAACATGG GCCTGCTCCA GCAACTTGAG GCTGATATTG GTATCACGCT CATTGCTGTG 
GATGAGGCTC ACTGTATTTC TGAGTGGGGG CATGATTTTA GGAATTCATT CAGGAAGTTG 
GGCTCCCTGA AGACAGCACT GCCAATGGTT CCAATCGTTG CACTTACTGC TACTGCAAGT 
TCCTCAATCC GGGAAGACAT TGTACGTTGC TTAAATCTGA GAAATCCTCA GATCACCTGT 
ACTGGTTTTG ATCGACCAAA CCTGTATTTA GAAGTTAGGC GAAAAACAGG GAATATCCTT 
CAGGATCTGC AGCCATTTCT TGTCAAAACA AGTTCCCACT GGGAATTTGA AGGTCCAACA 
ATCATCTACT GTCCTTCTAG AAAAATGACA CAACAAGTTA CAGGTGAACT TAGGAAACTG 
AATCTATCCT GTGGAACATA CCATGCGGGC ATGAGTTTTA ACACAAGGAA AGACATTCAT 
CATAGGTTTG TAAGAGATGA AATTCAGTGT GTCATAGCTA CCATAGCTTT TGGAATGGGC 
ATTAATAAAG CTGACATTCG CCAAGTCATT CATTACGGTG CTCCTAAGGA CATGGAATCA 
TATTATCAGG AGATTGGTAG AGCTGGTCGT GATGGACTTC AAAGTTCTTG TCACGTCCTC 
TGGGCTCCTG CAGACATTAA CTTAAATAGG CACCTTCTTA CTGAGATACG TAATGAGAAG 
TTTCGATTAT ACAAATTAAA GATGACGGCA AAGATGGAAA AATATCTTCA TTCTAGCAGA 
TGTAGGAGAC AAATCATCTT GTCTCATTTT GAGGACAAAC AAGTACAAAA AGCCTCCTTG 
GGAATTATGG GAACTGAAAA ATGCTGTGAT AATTGCAGGT CCAGATTGGA TCATTGCTAT 
TCCATGGATG ACTCAGAGGA TACATCCTGG GACTTTGGTC CACAAGCATT TAAGCTTTTG 
TCTGCTGTGG ACATCTTAGG CGAAAAATTT GGAATTGGGC TTCCAATTTT ATTTCTCCGA 
GGATCTAATT CTCAGCGTCT TGCCGATCAA TATCGCAGGC ACAGTTTATT TGGCACTGGC 
AAGGATCAAA CAGAGAGTTG GTGGAAGGCT TTTTCCCGTC AGCTGATCAC TGAGGGATTC 
TTGGAAGAAG TTTCTCGGTA TAACAAATTT ATGAAGATTT GCATCCTTAC GAAAGAGGGT 
AGAAATTGGC TTCATAAAGC TAATACAGAA TCTCAGAGCC TCATCCTTCA AGCTAATGAA 
GAATTGTGTC CAAAGAAGTT TCTTCTGCCT AGTTCAAAAA CTGTATCTTC GGGCACCAAA 
GAGCATTGTT ATAATCAAGT ACCAGTTGAA TTAACTACAG AGAAGAAGTC TAACTTGGAG 
AAGTTATATT CTTATAAACC ATGTGATAAG ATTTCTTCTG GGAGTAACAT TTCTAAAAAA 
AGTATCATGG TACAGTCACC AGAAAAAGCT TACAGTTCCT CAGAGCCTAT TATTTCGGCA 
CAAGAGCAGG AGACTCAGAT TGTGTTATAT GGCAAATTGG TAGAAGCTAG GCAGAAACAT 
GCCAATAAAA TGGATGTTCC CCCAGCTATT CTGGCAACCA ACAAGATACT GGTGGATATG 
GCCAAAATGA GACCAACTAC GGTTGAAAAC GTAAAAAGGA TTGATGGTGT TTCTGAAGGC 
AAAGCTGCCA TGTTGGCCCC TCTGTTGGAA GTCATCAAAC ATTTCTGCCA AACAAATAGT 
GTTCAGACAG ACCTCTTTTC AAGTACAAAA CCTCAAGAAG AACAGAAGAC GAGTCTGGTA 
GCAAAAAATA AAATATGCAC ACTTTCACAG TCTATGGCCA TCACATACTC TTTATTCCAA 
GAAAAGAAGA TGCCTTTGAA GAGCATAGCT GAGAGCAGGA TTCTGCCTCT TATGACAATT 
GGCATGCACT TATCCCAAGC GGTGAAAGCT GGCTGCCCCC TTGATTTGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTGCT GATGTTATCC GAAACCCTCC CGTCAACTCA 
GATATGAGTA AAATTAGCCT AATCAGAATG TTAGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG CAATTGAGAT CCTTAAACAT GGTCCTGACA GCGGACTTCA ACCTTCATGT 
GATGTCAACA AAAGGAGATG TTTTCCCAGT TCTGAAGAGA TCTGTTCAAG TTCTAAGAGA 
AGCAAGGAAG AAGTAGGCAT CAATACTGAG ACTTCATCTG CAGAGAGAAA GAGACGATTA 
CCTGTGTGGT TTGCCAAAGG AAGTGATACC AGCAAGAAAT TAATGGACAA AACGAAAAGG 
GGAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_016959317.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016814806.1 Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

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CloneID	OPc87750
Clone ID Related Accession (Same CDS sequence)	XM_016959317.1 , XM_016959317.2
Accession Version	XM_016959317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006475.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTAT CAGATGGGGA TGTGGTGGGA TTTGACATGG AGTGGCCACC ATTATACAAT 
AGAGGGAAAC TTGGCAAAGT TGCACTAATT CAGTTGTGTG TTTCTGAGAG CAAATGTTAC 
TTGTTCCACA TTTCTTCCAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
AAAGCAGTTA AAAAGGCAGG TGTAGGAATT GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGATATCA AATTGAAGAA TTTTGTGGAG TTGACAGATG TTGCCAATAA AAAGCTGAAA 
TGTACAGAGA CCTGGAGCCT CAACAGTCTG GTTAAACACC TCTTAGGTAA ACAGCTCCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTAAATTTC CTCTCACTGA GGACCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT TTTATTATTT ACCGAAATTT AGAGATTTTG 
GATGATACTG TGCAAAGGTT TGCTATAAAT AAAGAGGAAG AAATCCTACT TAGCGACATG 
AACAAACAGT TGACTTCAAT CTCTGAGGAA GTGATGGATC TGGCTAAGCA TCTTCCTCAT 
GCTTTCAGTA AACTGGAAAA CCCACGGAGG GTTTCTATCT TACTAAAGGA TATTTCAGAA 
AATCTATATT CACTGAGGAG GATGATAATT GGGTCTACTA ACATTGAGAC TGAACTGAGG 
CCCAGCAATA ATTTAAACTT ATTATCCTTT GGAGATTCAA CTACTGGGGG AGTACAACAG 
AAACAAATTA GAGAACATGA AGTTTTAATT CACGTTGCAG ATGAAACATG GGACCCAACA 
CTTGATCATT TAGCTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAGT GGAACGAAAA 
GAAGATGGAT TTGAAGATGG AGTAGAAGAC AACAAATTGA AAGAGAATAT GGAAAGAGCT 
TGTTTGATGT CGTTAGATAT TACAGAACAT GAACTCCAAA TTTTGGAACA GCAGGCTCAG 
GAAGAATATC TTAGTGATAC TGCTTATAAA TCTACTGAGC ATTTATCTCC CAATGATAAT 
GAAAACGATA CGTCCTATGT AATTGAGAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
CATTTATCTC CCAATAATGA TGAAAACGAT ACGTCCTATG TAATTGAGAG TGATGAAGAT 
TTAGAAATGG AGATGCTTAA GTCTTTAGAA AACCTCAACA GTGGCACGGT AGAACCAACT 
CATTCTAAAT GCTTAAAAAT GGAAAGAAAT CTGGGTCTTC TTACTAAAGA AGAAGAAGAA 
GATGATGAAA ATGAAGCTAA TGAAGGGGAA GAAGATGATG ATAAGGACTT TTTGTTGCCA 
GCACCCAATG AAGAGCAAGT TACTTGCCTC AAGATGTACT TTGGCCATTC CAGTTTTAAA 
CCAGTTCAGT GGAAAGTGAT TCATTCAGTA TTAGAAGAAA GAAGAGATAA TGTTGCTGTC 
ATGGCAACTG GATATGGAAA GAGTTTGTGC TTCCAGTATC CACCTGTTTA TGTAGGCAAG 
ATTGGCCTTG TTATCTCTCC CCTTATTTCT CTGATGGAAG ACCAAGTGCT ACAGCTTAAA 
ATGTCCAACA TCCCAGCTTG CTTCCTTGGA TCAGCACAGT CAGAAAATGT TCTAACAGAT 
ATTAAATTAG GTAAATACCG GATTGTATAC GTAACTCCAG AATACTGTTC AGGTAACATG 
GGCCTGCTCC AGCAACTTGA GGCTGATATT GGTATCACGC TCATTGCTGT GGATGAGGCT 
CACTGTATTT CTGAGTGGGG GCATGATTTT AGGAATTCAT TCAGGAAGTT GGGCTCCCTG 
AAGACAGCAC TGCCAATGGT TCCAATCGTT GCACTTACTG CTACTGCAAG TTCCTCAATC 
CGGGAAGACA TTGTACGTTG CTTAAATCTG AGAAATCCTC AGATCACCTG TACTGGTTTT 
GATCGACCAA ACCTGTATTT AGAAGTTAGG CGAAAAACAG GGAATATCCT TCAGGATCTG 
CAGCCATTTC TTGTCAAAAC AAGTTCCCAC TGGGAATTTG AAGGTCCAAC AATCATCTAC 
TGTCCTTCTA GAAAAATGAC ACAACAAGTT ACAGGTGAAC TTAGGAAACT GAATCTATCC 
TGTGGAACAT ACCATGCGGG CATGAGTTTT AACACAAGGA AAGACATTCA TCATAGGTTT 
GTAAGAGATG AAATTCAGTG TGTCATAGCT ACCATAGCTT TTGGAATGGG CATTAATAAA 
GCTGACATTC GCCAAGTCAT TCATTACGGT GCTCCTAAGG ACATGGAATC ATATTATCAG 
GAGATTGGTA GAGCTGGTCG TGATGGACTT CAAAGTTCTT GTCACGTCCT CTGGGCTCCT 
GCAGACATTA ACTTAAATAG GCACCTTCTT ACTGAGATAC GTAATGAGAA GTTTCGATTA 
TACAAATTAA AGATGACGGC AAAGATGGAA AAATATCTTC ATTCTAGCAG ATGTAGGAGA 
CAAATCATCT TGTCTCATTT TGAGGACAAA CAAGTACAAA AAGCCTCCTT GGGAATTATG 
GGAACTGAAA AATGCTGTGA TAATTGCAGG TCCAGATTGG ATCATTGCTA TTCCATGGAT 
GACTCAGAGG ATACATCCTG GGACTTTGGT CCACAAGCAT TTAAGCTTTT GTCTGCTGTG 
GACATCTTAG GCGAAAAATT TGGAATTGGG CTTCCAATTT TATTTCTCCG AGGATCTAAT 
TCTCAGCGTC TTGCCGATCA ATATCGCAGG CACAGTTTAT TTGGCACTGG CAAGGATCAA 
ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT CAGCTGATCA CTGAGGGATT CTTGGAAGAA 
GTTTCTCGGT ATAACAAATT TATGAAGATT TGCATCCTTA CGAAAGAGGG TAGAAATTGG 
CTTCATAAAG CTAATACAGA ATCTCAGAGC CTCATCCTTC AAGCTAATGA AGAATTGTGT 
CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA ACTGTATCTT CGGGCACCAA AGAGCATTGT 
TATAATCAAG TACCAGTTGA ATTAACTACA GAGAAGAAGT CTAACTTGGA GAAGTTATAT 
TCTTATAAAC CATGTGATAA GATTTCTTCT GGGAGTAACA TTTCTAAAAA AAGTATCATG 
GTACAGTCAC CAGAAAAAGC TTACAGTTCC TCAGAGCCTA TTATTTCGGC ACAAGAGCAG 
GAGACTCAGA TTGTGTTATA TGGCAAATTG GTAGAAGCTA GGCAGAAACA TGCCAATAAA 
ATGGATGTTC CCCCAGCTAT TCTGGCAACC AACAAGATAC TGGTGGATAT GGCCAAAATG 
AGACCAACTA CGGTTGAAAA CGTAAAAAGG ATTGATGGTG TTTCTGAAGG CAAAGCTGCC 
ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCTGCC AAACAAATAG TGTTCAGACA 
GACCTCTTTT CAAGTACAAA ACCTCAAGAA GAACAGAAGA CGAGTCTGGT AGCAAAAAAT 
AAAATATGCA CACTTTCACA GTCTATGGCC ATCACATACT CTTTATTCCA AGAAAAGAAG 
ATGCCTTTGA AGAGCATAGC TGAGAGCAGG ATTCTGCCTC TTATGACAAT TGGCATGCAC 
TTATCCCAAG CGGTGAAAGC TGGCTGCCCC CTTGATTTGG AGCGAGCAGG CCTGACTCCA 
GAGGTTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCGTCAACTC AGATATGAGT 
AAAATTAGCC TAATCAGAAT GTTAGTTCCT GAAAACATTG ACACGTACCT TATCCACATG 
GCAATTGAGA TCCTTAAACA TGGTCCTGAC AGCGGACTTC AACCTTCATG TGATGTCAAC 
AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA 
GAAGTAGGCA TCAATACTGA GACTTCATCT GCAGAGAGAA AGAGACGATT ACCTGTGTGG 
TTTGCCAAAG GAAGTGATAC CAGCAAGAAA TTAATGGACA AAACGAAAAG GGGAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016814806.1
CDS	263..4351
Translation	MSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHISSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFGDSTTGGVQQKQIREHEVLIHVADETWDPTLDHLAKHDGEGVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQAQEEYLSDTAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNNDENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLLTKEEEEDDENEANEGEEDDDKDFLLPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRNSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFNTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMTAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLEEVSRYNKFMKICILTKEGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQVPVELTTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSEPIISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPSSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

Target ORF information:

RefSeq Version	XM_016959317.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016959317.1

ORF Insert Sequence:

ATGAGTCTAT CAGATGGGGA TGTGGTGGGA TTTGACATGG AGTGGCCACC ATTATACAAT 
AGAGGGAAAC TTGGCAAAGT TGCACTAATT CAGTTGTGTG TTTCTGAGAG CAAATGTTAC 
TTGTTCCACA TTTCTTCCAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
AAAGCAGTTA AAAAGGCAGG TGTAGGAATT GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGATATCA AATTGAAGAA TTTTGTGGAG TTGACAGATG TTGCCAATAA AAAGCTGAAA 
TGTACAGAGA CCTGGAGCCT CAACAGTCTG GTTAAACACC TCTTAGGTAA ACAGCTCCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTAAATTTC CTCTCACTGA GGACCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT TTTATTATTT ACCGAAATTT AGAGATTTTG 
GATGATACTG TGCAAAGGTT TGCTATAAAT AAAGAGGAAG AAATCCTACT TAGCGACATG 
AACAAACAGT TGACTTCAAT CTCTGAGGAA GTGATGGATC TGGCTAAGCA TCTTCCTCAT 
GCTTTCAGTA AACTGGAAAA CCCACGGAGG GTTTCTATCT TACTAAAGGA TATTTCAGAA 
AATCTATATT CACTGAGGAG GATGATAATT GGGTCTACTA ACATTGAGAC TGAACTGAGG 
CCCAGCAATA ATTTAAACTT ATTATCCTTT GGAGATTCAA CTACTGGGGG AGTACAACAG 
AAACAAATTA GAGAACATGA AGTTTTAATT CACGTTGCAG ATGAAACATG GGACCCAACA 
CTTGATCATT TAGCTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAGT GGAACGAAAA 
GAAGATGGAT TTGAAGATGG AGTAGAAGAC AACAAATTGA AAGAGAATAT GGAAAGAGCT 
TGTTTGATGT CGTTAGATAT TACAGAACAT GAACTCCAAA TTTTGGAACA GCAGGCTCAG 
GAAGAATATC TTAGTGATAC TGCTTATAAA TCTACTGAGC ATTTATCTCC CAATGATAAT 
GAAAACGATA CGTCCTATGT AATTGAGAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
CATTTATCTC CCAATAATGA TGAAAACGAT ACGTCCTATG TAATTGAGAG TGATGAAGAT 
TTAGAAATGG AGATGCTTAA GTCTTTAGAA AACCTCAACA GTGGCACGGT AGAACCAACT 
CATTCTAAAT GCTTAAAAAT GGAAAGAAAT CTGGGTCTTC TTACTAAAGA AGAAGAAGAA 
GATGATGAAA ATGAAGCTAA TGAAGGGGAA GAAGATGATG ATAAGGACTT TTTGTTGCCA 
GCACCCAATG AAGAGCAAGT TACTTGCCTC AAGATGTACT TTGGCCATTC CAGTTTTAAA 
CCAGTTCAGT GGAAAGTGAT TCATTCAGTA TTAGAAGAAA GAAGAGATAA TGTTGCTGTC 
ATGGCAACTG GATATGGAAA GAGTTTGTGC TTCCAGTATC CACCTGTTTA TGTAGGCAAG 
ATTGGCCTTG TTATCTCTCC CCTTATTTCT CTGATGGAAG ACCAAGTGCT ACAGCTTAAA 
ATGTCCAACA TCCCAGCTTG CTTCCTTGGA TCAGCACAGT CAGAAAATGT TCTAACAGAT 
ATTAAATTAG GTAAATACCG GATTGTATAC GTAACTCCAG AATACTGTTC AGGTAACATG 
GGCCTGCTCC AGCAACTTGA GGCTGATATT GGTATCACGC TCATTGCTGT GGATGAGGCT 
CACTGTATTT CTGAGTGGGG GCATGATTTT AGGAATTCAT TCAGGAAGTT GGGCTCCCTG 
AAGACAGCAC TGCCAATGGT TCCAATCGTT GCACTTACTG CTACTGCAAG TTCCTCAATC 
CGGGAAGACA TTGTACGTTG CTTAAATCTG AGAAATCCTC AGATCACCTG TACTGGTTTT 
GATCGACCAA ACCTGTATTT AGAAGTTAGG CGAAAAACAG GGAATATCCT TCAGGATCTG 
CAGCCATTTC TTGTCAAAAC AAGTTCCCAC TGGGAATTTG AAGGTCCAAC AATCATCTAC 
TGTCCTTCTA GAAAAATGAC ACAACAAGTT ACAGGTGAAC TTAGGAAACT GAATCTATCC 
TGTGGAACAT ACCATGCGGG CATGAGTTTT AACACAAGGA AAGACATTCA TCATAGGTTT 
GTAAGAGATG AAATTCAGTG TGTCATAGCT ACCATAGCTT TTGGAATGGG CATTAATAAA 
GCTGACATTC GCCAAGTCAT TCATTACGGT GCTCCTAAGG ACATGGAATC ATATTATCAG 
GAGATTGGTA GAGCTGGTCG TGATGGACTT CAAAGTTCTT GTCACGTCCT CTGGGCTCCT 
GCAGACATTA ACTTAAATAG GCACCTTCTT ACTGAGATAC GTAATGAGAA GTTTCGATTA 
TACAAATTAA AGATGACGGC AAAGATGGAA AAATATCTTC ATTCTAGCAG ATGTAGGAGA 
CAAATCATCT TGTCTCATTT TGAGGACAAA CAAGTACAAA AAGCCTCCTT GGGAATTATG 
GGAACTGAAA AATGCTGTGA TAATTGCAGG TCCAGATTGG ATCATTGCTA TTCCATGGAT 
GACTCAGAGG ATACATCCTG GGACTTTGGT CCACAAGCAT TTAAGCTTTT GTCTGCTGTG 
GACATCTTAG GCGAAAAATT TGGAATTGGG CTTCCAATTT TATTTCTCCG AGGATCTAAT 
TCTCAGCGTC TTGCCGATCA ATATCGCAGG CACAGTTTAT TTGGCACTGG CAAGGATCAA 
ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT CAGCTGATCA CTGAGGGATT CTTGGAAGAA 
GTTTCTCGGT ATAACAAATT TATGAAGATT TGCATCCTTA CGAAAGAGGG TAGAAATTGG 
CTTCATAAAG CTAATACAGA ATCTCAGAGC CTCATCCTTC AAGCTAATGA AGAATTGTGT 
CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA ACTGTATCTT CGGGCACCAA AGAGCATTGT 
TATAATCAAG TACCAGTTGA ATTAACTACA GAGAAGAAGT CTAACTTGGA GAAGTTATAT 
TCTTATAAAC CATGTGATAA GATTTCTTCT GGGAGTAACA TTTCTAAAAA AAGTATCATG 
GTACAGTCAC CAGAAAAAGC TTACAGTTCC TCAGAGCCTA TTATTTCGGC ACAAGAGCAG 
GAGACTCAGA TTGTGTTATA TGGCAAATTG GTAGAAGCTA GGCAGAAACA TGCCAATAAA 
ATGGATGTTC CCCCAGCTAT TCTGGCAACC AACAAGATAC TGGTGGATAT GGCCAAAATG 
AGACCAACTA CGGTTGAAAA CGTAAAAAGG ATTGATGGTG TTTCTGAAGG CAAAGCTGCC 
ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCTGCC AAACAAATAG TGTTCAGACA 
GACCTCTTTT CAAGTACAAA ACCTCAAGAA GAACAGAAGA CGAGTCTGGT AGCAAAAAAT 
AAAATATGCA CACTTTCACA GTCTATGGCC ATCACATACT CTTTATTCCA AGAAAAGAAG 
ATGCCTTTGA AGAGCATAGC TGAGAGCAGG ATTCTGCCTC TTATGACAAT TGGCATGCAC 
TTATCCCAAG CGGTGAAAGC TGGCTGCCCC CTTGATTTGG AGCGAGCAGG CCTGACTCCA 
GAGGTTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCGTCAACTC AGATATGAGT 
AAAATTAGCC TAATCAGAAT GTTAGTTCCT GAAAACATTG ACACGTACCT TATCCACATG 
GCAATTGAGA TCCTTAAACA TGGTCCTGAC AGCGGACTTC AACCTTCATG TGATGTCAAC 
AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA 
GAAGTAGGCA TCAATACTGA GACTTCATCT GCAGAGAGAA AGAGACGATT ACCTGTGTGG 
TTTGCCAAAG GAAGTGATAC CAGCAAGAAA TTAATGGACA AAACGAAAAG GGGAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_016959317.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016814806.1 Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

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CloneID	OPc87750
Clone ID Related Accession (Same CDS sequence)	XM_016959317.1 , XM_016959317.2
Accession Version	XM_016959317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016959317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTAT CAGATGGGGA TGTGGTGGGA TTTGACATGG AGTGGCCACC ATTATACAAT 
AGAGGGAAAC TTGGCAAAGT TGCACTAATT CAGTTGTGTG TTTCTGAGAG CAAATGTTAC 
TTGTTCCACA TTTCTTCCAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
AAAGCAGTTA AAAAGGCAGG TGTAGGAATT GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGATATCA AATTGAAGAA TTTTGTGGAG TTGACAGATG TTGCCAATAA AAAGCTGAAA 
TGTACAGAGA CCTGGAGCCT CAACAGTCTG GTTAAACACC TCTTAGGTAA ACAGCTCCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTAAATTTC CTCTCACTGA GGACCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT TTTATTATTT ACCGAAATTT AGAGATTTTG 
GATGATACTG TGCAAAGGTT TGCTATAAAT AAAGAGGAAG AAATCCTACT TAGCGACATG 
AACAAACAGT TGACTTCAAT CTCTGAGGAA GTGATGGATC TGGCTAAGCA TCTTCCTCAT 
GCTTTCAGTA AACTGGAAAA CCCACGGAGG GTTTCTATCT TACTAAAGGA TATTTCAGAA 
AATCTATATT CACTGAGGAG GATGATAATT GGGTCTACTA ACATTGAGAC TGAACTGAGG 
CCCAGCAATA ATTTAAACTT ATTATCCTTT GGAGATTCAA CTACTGGGGG AGTACAACAG 
AAACAAATTA GAGAACATGA AGTTTTAATT CACGTTGCAG ATGAAACATG GGACCCAACA 
CTTGATCATT TAGCTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAGT GGAACGAAAA 
GAAGATGGAT TTGAAGATGG AGTAGAAGAC AACAAATTGA AAGAGAATAT GGAAAGAGCT 
TGTTTGATGT CGTTAGATAT TACAGAACAT GAACTCCAAA TTTTGGAACA GCAGGCTCAG 
GAAGAATATC TTAGTGATAC TGCTTATAAA TCTACTGAGC ATTTATCTCC CAATGATAAT 
GAAAACGATA CGTCCTATGT AATTGAGAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
CATTTATCTC CCAATAATGA TGAAAACGAT ACGTCCTATG TAATTGAGAG TGATGAAGAT 
TTAGAAATGG AGATGCTTAA GTCTTTAGAA AACCTCAACA GTGGCACGGT AGAACCAACT 
CATTCTAAAT GCTTAAAAAT GGAAAGAAAT CTGGGTCTTC TTACTAAAGA AGAAGAAGAA 
GATGATGAAA ATGAAGCTAA TGAAGGGGAA GAAGATGATG ATAAGGACTT TTTGTTGCCA 
GCACCCAATG AAGAGCAAGT TACTTGCCTC AAGATGTACT TTGGCCATTC CAGTTTTAAA 
CCAGTTCAGT GGAAAGTGAT TCATTCAGTA TTAGAAGAAA GAAGAGATAA TGTTGCTGTC 
ATGGCAACTG GATATGGAAA GAGTTTGTGC TTCCAGTATC CACCTGTTTA TGTAGGCAAG 
ATTGGCCTTG TTATCTCTCC CCTTATTTCT CTGATGGAAG ACCAAGTGCT ACAGCTTAAA 
ATGTCCAACA TCCCAGCTTG CTTCCTTGGA TCAGCACAGT CAGAAAATGT TCTAACAGAT 
ATTAAATTAG GTAAATACCG GATTGTATAC GTAACTCCAG AATACTGTTC AGGTAACATG 
GGCCTGCTCC AGCAACTTGA GGCTGATATT GGTATCACGC TCATTGCTGT GGATGAGGCT 
CACTGTATTT CTGAGTGGGG GCATGATTTT AGGAATTCAT TCAGGAAGTT GGGCTCCCTG 
AAGACAGCAC TGCCAATGGT TCCAATCGTT GCACTTACTG CTACTGCAAG TTCCTCAATC 
CGGGAAGACA TTGTACGTTG CTTAAATCTG AGAAATCCTC AGATCACCTG TACTGGTTTT 
GATCGACCAA ACCTGTATTT AGAAGTTAGG CGAAAAACAG GGAATATCCT TCAGGATCTG 
CAGCCATTTC TTGTCAAAAC AAGTTCCCAC TGGGAATTTG AAGGTCCAAC AATCATCTAC 
TGTCCTTCTA GAAAAATGAC ACAACAAGTT ACAGGTGAAC TTAGGAAACT GAATCTATCC 
TGTGGAACAT ACCATGCGGG CATGAGTTTT AACACAAGGA AAGACATTCA TCATAGGTTT 
GTAAGAGATG AAATTCAGTG TGTCATAGCT ACCATAGCTT TTGGAATGGG CATTAATAAA 
GCTGACATTC GCCAAGTCAT TCATTACGGT GCTCCTAAGG ACATGGAATC ATATTATCAG 
GAGATTGGTA GAGCTGGTCG TGATGGACTT CAAAGTTCTT GTCACGTCCT CTGGGCTCCT 
GCAGACATTA ACTTAAATAG GCACCTTCTT ACTGAGATAC GTAATGAGAA GTTTCGATTA 
TACAAATTAA AGATGACGGC AAAGATGGAA AAATATCTTC ATTCTAGCAG ATGTAGGAGA 
CAAATCATCT TGTCTCATTT TGAGGACAAA CAAGTACAAA AAGCCTCCTT GGGAATTATG 
GGAACTGAAA AATGCTGTGA TAATTGCAGG TCCAGATTGG ATCATTGCTA TTCCATGGAT 
GACTCAGAGG ATACATCCTG GGACTTTGGT CCACAAGCAT TTAAGCTTTT GTCTGCTGTG 
GACATCTTAG GCGAAAAATT TGGAATTGGG CTTCCAATTT TATTTCTCCG AGGATCTAAT 
TCTCAGCGTC TTGCCGATCA ATATCGCAGG CACAGTTTAT TTGGCACTGG CAAGGATCAA 
ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT CAGCTGATCA CTGAGGGATT CTTGGAAGAA 
GTTTCTCGGT ATAACAAATT TATGAAGATT TGCATCCTTA CGAAAGAGGG TAGAAATTGG 
CTTCATAAAG CTAATACAGA ATCTCAGAGC CTCATCCTTC AAGCTAATGA AGAATTGTGT 
CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA ACTGTATCTT CGGGCACCAA AGAGCATTGT 
TATAATCAAG TACCAGTTGA ATTAACTACA GAGAAGAAGT CTAACTTGGA GAAGTTATAT 
TCTTATAAAC CATGTGATAA GATTTCTTCT GGGAGTAACA TTTCTAAAAA AAGTATCATG 
GTACAGTCAC CAGAAAAAGC TTACAGTTCC TCAGAGCCTA TTATTTCGGC ACAAGAGCAG 
GAGACTCAGA TTGTGTTATA TGGCAAATTG GTAGAAGCTA GGCAGAAACA TGCCAATAAA 
ATGGATGTTC CCCCAGCTAT TCTGGCAACC AACAAGATAC TGGTGGATAT GGCCAAAATG 
AGACCAACTA CGGTTGAAAA CGTAAAAAGG ATTGATGGTG TTTCTGAAGG CAAAGCTGCC 
ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCTGCC AAACAAATAG TGTTCAGACA 
GACCTCTTTT CAAGTACAAA ACCTCAAGAA GAACAGAAGA CGAGTCTGGT AGCAAAAAAT 
AAAATATGCA CACTTTCACA GTCTATGGCC ATCACATACT CTTTATTCCA AGAAAAGAAG 
ATGCCTTTGA AGAGCATAGC TGAGAGCAGG ATTCTGCCTC TTATGACAAT TGGCATGCAC 
TTATCCCAAG CGGTGAAAGC TGGCTGCCCC CTTGATTTGG AGCGAGCAGG CCTGACTCCA 
GAGGTTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCGTCAACTC AGATATGAGT 
AAAATTAGCC TAATCAGAAT GTTAGTTCCT GAAAACATTG ACACGTACCT TATCCACATG 
GCAATTGAGA TCCTTAAACA TGGTCCTGAC AGCGGACTTC AACCTTCATG TGATGTCAAC 
AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA 
GAAGTAGGCA TCAATACTGA GACTTCATCT GCAGAGAGAA AGAGACGATT ACCTGTGTGG 
TTTGCCAAAG GAAGTGATAC CAGCAAGAAA TTAATGGACA AAACGAAAAG GGGAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016814806.1
CDS	263..4351
Translation	MSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHISSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFGDSTTGGVQQKQIREHEVLIHVADETWDPTLDHLAKHDGEGVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQAQEEYLSDTAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNNDENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLLTKEEEEDDENEANEGEEDDDKDFLLPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRNSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFNTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMTAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLEEVSRYNKFMKICILTKEGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQVPVELTTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSEPIISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPSSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

Target ORF information:

RefSeq Version	XM_016959317.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016959317.2

ORF Insert Sequence:

ATGAGTCTAT CAGATGGGGA TGTGGTGGGA TTTGACATGG AGTGGCCACC ATTATACAAT 
AGAGGGAAAC TTGGCAAAGT TGCACTAATT CAGTTGTGTG TTTCTGAGAG CAAATGTTAC 
TTGTTCCACA TTTCTTCCAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
AAAGCAGTTA AAAAGGCAGG TGTAGGAATT GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGATATCA AATTGAAGAA TTTTGTGGAG TTGACAGATG TTGCCAATAA AAAGCTGAAA 
TGTACAGAGA CCTGGAGCCT CAACAGTCTG GTTAAACACC TCTTAGGTAA ACAGCTCCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTAAATTTC CTCTCACTGA GGACCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT TTTATTATTT ACCGAAATTT AGAGATTTTG 
GATGATACTG TGCAAAGGTT TGCTATAAAT AAAGAGGAAG AAATCCTACT TAGCGACATG 
AACAAACAGT TGACTTCAAT CTCTGAGGAA GTGATGGATC TGGCTAAGCA TCTTCCTCAT 
GCTTTCAGTA AACTGGAAAA CCCACGGAGG GTTTCTATCT TACTAAAGGA TATTTCAGAA 
AATCTATATT CACTGAGGAG GATGATAATT GGGTCTACTA ACATTGAGAC TGAACTGAGG 
CCCAGCAATA ATTTAAACTT ATTATCCTTT GGAGATTCAA CTACTGGGGG AGTACAACAG 
AAACAAATTA GAGAACATGA AGTTTTAATT CACGTTGCAG ATGAAACATG GGACCCAACA 
CTTGATCATT TAGCTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAGT GGAACGAAAA 
GAAGATGGAT TTGAAGATGG AGTAGAAGAC AACAAATTGA AAGAGAATAT GGAAAGAGCT 
TGTTTGATGT CGTTAGATAT TACAGAACAT GAACTCCAAA TTTTGGAACA GCAGGCTCAG 
GAAGAATATC TTAGTGATAC TGCTTATAAA TCTACTGAGC ATTTATCTCC CAATGATAAT 
GAAAACGATA CGTCCTATGT AATTGAGAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
CATTTATCTC CCAATAATGA TGAAAACGAT ACGTCCTATG TAATTGAGAG TGATGAAGAT 
TTAGAAATGG AGATGCTTAA GTCTTTAGAA AACCTCAACA GTGGCACGGT AGAACCAACT 
CATTCTAAAT GCTTAAAAAT GGAAAGAAAT CTGGGTCTTC TTACTAAAGA AGAAGAAGAA 
GATGATGAAA ATGAAGCTAA TGAAGGGGAA GAAGATGATG ATAAGGACTT TTTGTTGCCA 
GCACCCAATG AAGAGCAAGT TACTTGCCTC AAGATGTACT TTGGCCATTC CAGTTTTAAA 
CCAGTTCAGT GGAAAGTGAT TCATTCAGTA TTAGAAGAAA GAAGAGATAA TGTTGCTGTC 
ATGGCAACTG GATATGGAAA GAGTTTGTGC TTCCAGTATC CACCTGTTTA TGTAGGCAAG 
ATTGGCCTTG TTATCTCTCC CCTTATTTCT CTGATGGAAG ACCAAGTGCT ACAGCTTAAA 
ATGTCCAACA TCCCAGCTTG CTTCCTTGGA TCAGCACAGT CAGAAAATGT TCTAACAGAT 
ATTAAATTAG GTAAATACCG GATTGTATAC GTAACTCCAG AATACTGTTC AGGTAACATG 
GGCCTGCTCC AGCAACTTGA GGCTGATATT GGTATCACGC TCATTGCTGT GGATGAGGCT 
CACTGTATTT CTGAGTGGGG GCATGATTTT AGGAATTCAT TCAGGAAGTT GGGCTCCCTG 
AAGACAGCAC TGCCAATGGT TCCAATCGTT GCACTTACTG CTACTGCAAG TTCCTCAATC 
CGGGAAGACA TTGTACGTTG CTTAAATCTG AGAAATCCTC AGATCACCTG TACTGGTTTT 
GATCGACCAA ACCTGTATTT AGAAGTTAGG CGAAAAACAG GGAATATCCT TCAGGATCTG 
CAGCCATTTC TTGTCAAAAC AAGTTCCCAC TGGGAATTTG AAGGTCCAAC AATCATCTAC 
TGTCCTTCTA GAAAAATGAC ACAACAAGTT ACAGGTGAAC TTAGGAAACT GAATCTATCC 
TGTGGAACAT ACCATGCGGG CATGAGTTTT AACACAAGGA AAGACATTCA TCATAGGTTT 
GTAAGAGATG AAATTCAGTG TGTCATAGCT ACCATAGCTT TTGGAATGGG CATTAATAAA 
GCTGACATTC GCCAAGTCAT TCATTACGGT GCTCCTAAGG ACATGGAATC ATATTATCAG 
GAGATTGGTA GAGCTGGTCG TGATGGACTT CAAAGTTCTT GTCACGTCCT CTGGGCTCCT 
GCAGACATTA ACTTAAATAG GCACCTTCTT ACTGAGATAC GTAATGAGAA GTTTCGATTA 
TACAAATTAA AGATGACGGC AAAGATGGAA AAATATCTTC ATTCTAGCAG ATGTAGGAGA 
CAAATCATCT TGTCTCATTT TGAGGACAAA CAAGTACAAA AAGCCTCCTT GGGAATTATG 
GGAACTGAAA AATGCTGTGA TAATTGCAGG TCCAGATTGG ATCATTGCTA TTCCATGGAT 
GACTCAGAGG ATACATCCTG GGACTTTGGT CCACAAGCAT TTAAGCTTTT GTCTGCTGTG 
GACATCTTAG GCGAAAAATT TGGAATTGGG CTTCCAATTT TATTTCTCCG AGGATCTAAT 
TCTCAGCGTC TTGCCGATCA ATATCGCAGG CACAGTTTAT TTGGCACTGG CAAGGATCAA 
ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT CAGCTGATCA CTGAGGGATT CTTGGAAGAA 
GTTTCTCGGT ATAACAAATT TATGAAGATT TGCATCCTTA CGAAAGAGGG TAGAAATTGG 
CTTCATAAAG CTAATACAGA ATCTCAGAGC CTCATCCTTC AAGCTAATGA AGAATTGTGT 
CCAAAGAAGT TTCTTCTGCC TAGTTCAAAA ACTGTATCTT CGGGCACCAA AGAGCATTGT 
TATAATCAAG TACCAGTTGA ATTAACTACA GAGAAGAAGT CTAACTTGGA GAAGTTATAT 
TCTTATAAAC CATGTGATAA GATTTCTTCT GGGAGTAACA TTTCTAAAAA AAGTATCATG 
GTACAGTCAC CAGAAAAAGC TTACAGTTCC TCAGAGCCTA TTATTTCGGC ACAAGAGCAG 
GAGACTCAGA TTGTGTTATA TGGCAAATTG GTAGAAGCTA GGCAGAAACA TGCCAATAAA 
ATGGATGTTC CCCCAGCTAT TCTGGCAACC AACAAGATAC TGGTGGATAT GGCCAAAATG 
AGACCAACTA CGGTTGAAAA CGTAAAAAGG ATTGATGGTG TTTCTGAAGG CAAAGCTGCC 
ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCTGCC AAACAAATAG TGTTCAGACA 
GACCTCTTTT CAAGTACAAA ACCTCAAGAA GAACAGAAGA CGAGTCTGGT AGCAAAAAAT 
AAAATATGCA CACTTTCACA GTCTATGGCC ATCACATACT CTTTATTCCA AGAAAAGAAG 
ATGCCTTTGA AGAGCATAGC TGAGAGCAGG ATTCTGCCTC TTATGACAAT TGGCATGCAC 
TTATCCCAAG CGGTGAAAGC TGGCTGCCCC CTTGATTTGG AGCGAGCAGG CCTGACTCCA 
GAGGTTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCGTCAACTC AGATATGAGT 
AAAATTAGCC TAATCAGAAT GTTAGTTCCT GAAAACATTG ACACGTACCT TATCCACATG 
GCAATTGAGA TCCTTAAACA TGGTCCTGAC AGCGGACTTC AACCTTCATG TGATGTCAAC 
AAAAGGAGAT GTTTTCCCAG TTCTGAAGAG ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA 
GAAGTAGGCA TCAATACTGA GACTTCATCT GCAGAGAGAA AGAGACGATT ACCTGTGTGG 
TTTGCCAAAG GAAGTGATAC CAGCAAGAAA TTAATGGACA AAACGAAAAG GGGAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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WRN cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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