PLCE1 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCE1
Entrez Gene ID	100992707
Full Name	phospholipase C epsilon 1
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003825376.2	XP_003825424.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Pathways from BioSystems
Gene Ontology

The following PLCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa58728	XM_003825376.2 Latest version!	Pan paniscus phospholipase C epsilon 1 (PLCE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCE1 ( XM_003825376.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003825424.1 Pan paniscus phospholipase C epsilon 1 (PLCE1), mRNA.

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CloneID	OPa58728
Clone ID Related Accession (Same CDS sequence)	XM_003825376.2
Accession Version	XM_003825376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027878.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 30, 2015 this sequence version replaced XM_003825376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCATGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGTTCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCA GCCTCATGAA GCTTTGCCCA 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCGTGGTT CAGAGGATTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGTTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGTGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTATGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCGTT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCTGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTTTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGCGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCC ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GACAACATTC TGGAAGACAG ACCTGAAAAT 
AAATCATGTA ATGACAAGCT TCAGTTTGAA TATAATGAAG AAATCCCAAA GAGGATAAAG 
AAAGCAGATA ACTCTGCTTG CAACAAAGGA AAGGTTTATG ATATGGAACT GGGAGAAGAA 
TTTTATCTTG ATCAGAATAA AAAGGAAAGC AGACAGATTG CACCAGAGCT TTCTGACCTT 
GTAATCTATT GTCAAGCAGT AAAATTTCCA GGACTGTCAA CTCTAAATGC ATCTGGCTCT 
AGCAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGAATG 
AGCCCAGGGG AGACAGCATC ATTTAACAAA ACATCTGGAA AAAGTTCCTG TGAAGGCATT 
CGACAGACCT GGGAGGAATC TTCTTCCCCT CTCAACCCAG CCACGTCCCT CAGTGCTATC 
ATTAGAACTC CCAAATGTTA TCATATCTCG TCGCTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGATCCAG CACACCGCCT GTCAGCTGCT GAGAACTTAC 
CCTGCTGCCA CCCGCATCGA CTCTTCCAAC CCGAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTCG TGGCACTCAA CTACCAGACC GATGATCTCC CTTTACATTT AAATGCTGCA 
ATGTTTGAGG CAAATGGTGG TTGTGGTTAT GTTTTGAAAC CTCCAGTTCT GTGGGACAAG 
AACTGCCCCA TGTATCAGAA GTTTTCTCCA CTAGAAAGAG ATCTGGACAG CATGGATCCT 
GCAGTCTATT CTTTAACTAT TGTCTCTGGT CAGAATGTGT GCCCCAGTAA TAGCATGGGA 
AGCCCGTGCA TTGAAGTCGA CGTCCTGGGC ATGCCTCTGG ACAGCTGCCA TTTCCGCACA 
AAGCCCATCC ATCGAAACAC CCTGAACCCC ATGTGGAACG AGCAGTTTCT GTTCCGCGTT 
CACTTCGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AAAACAATAG TTCAGCGGTA 
ACTGCTCAGA GAATCATTCC ACTGAAAGCT TTAAAACGAG GATATCGACA TCTTCAGCTG 
CGAAACCTTC ACAATGAAGT CTTGGAGATT TCTAGTTTAT TCATTAACAG CAGAAGGATG 
GAAGAAAATT CCTCTGGCAA TACCATGTCA GCCTCTTCGA TGTTTAATAC AGAAGAAAGA 
AAATGTTTGC AGACTCACAG AGTCACGGTG CATGGGGTCC CAGGGCCAGA GCCCTTTACC 
GTTTTCACTA TTAATGGAGG CACCAAGGCA AAGCAGCTTC TGCAGCAAAT TCTGACAAAT 
GAACAAGACA TCAAACCTGT TACCACAGAC TATTTTTTGA TGGAAGAAAA ATATTTTATA 
TCTAAAGAAA AGAATGAATG TAGGAAACAA CCATTCCAGA GAGCCATTGG TCCAGAAGAG 
GAGATCATGC AAATTTTAAG CAGCTGGTTT CCAGAAGAGG GATACGTGGG CAGGATTGTC 
TTAAAAACCC AGCAGGAAAA CCTAGAAGAG AAAAACATTG TTCAAGATGA CAAAGAGGTG 
ATCTTGAGCT CAGAGGAGGA GAGTTTCTTT GTCCAAGTGC ATGATGTTTC TCCAGAGCAA 
CCTCGAACAG TCATCAAAGC ACCCCGCGTC AGCACTGCAC AGGATGTCAT TCAGCAGACC 
TTATGCAAAG CCAAATATTC CTACAGCATC CTGAGCAACC CCAATCCAAG CGACTATGTG 
CTTTTGGAAG AGGTGGTGAA AGACACTACC AACAAGAAGA CCACCACACC AAAGTCCTCT 
CAGCGGGTCC TTCTGGATCA GGAGTGTGTG TTTCAAGCCC AAAGCAAGTG GAAAGGTGCA 
GGAAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CTCGAGAAGA TAAAAAGAAA 
GGCATTTCTT TCGCAAGTGA ACTCAAGAAG CTCACCAAGT CAACTAAACA GCCCCGAGGA 
CTTACATCAC CTTCTCAGCT CTTGACCTCA GAAAGTATCC AAACCAAGGA GGAGAAACCT 
GTGGGTGGCT TGTCCTCCAG TGACACAATG GATTACCGAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003825424.1
CDS	1..5985
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASSLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSRGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAYNGGNANPPPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPATSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYVGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_003825376.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus phospholipase C epsilon 1 (PLCE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003825376.2

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCATGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGTTCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCA GCCTCATGAA GCTTTGCCCA 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCGTGGTT CAGAGGATTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGTTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGTGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTATGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCGTT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCTGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTTTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGCGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCC ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GACAACATTC TGGAAGACAG ACCTGAAAAT 
AAATCATGTA ATGACAAGCT TCAGTTTGAA TATAATGAAG AAATCCCAAA GAGGATAAAG 
AAAGCAGATA ACTCTGCTTG CAACAAAGGA AAGGTTTATG ATATGGAACT GGGAGAAGAA 
TTTTATCTTG ATCAGAATAA AAAGGAAAGC AGACAGATTG CACCAGAGCT TTCTGACCTT 
GTAATCTATT GTCAAGCAGT AAAATTTCCA GGACTGTCAA CTCTAAATGC ATCTGGCTCT 
AGCAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGAATG 
AGCCCAGGGG AGACAGCATC ATTTAACAAA ACATCTGGAA AAAGTTCCTG TGAAGGCATT 
CGACAGACCT GGGAGGAATC TTCTTCCCCT CTCAACCCAG CCACGTCCCT CAGTGCTATC 
ATTAGAACTC CCAAATGTTA TCATATCTCG TCGCTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGATCCAG CACACCGCCT GTCAGCTGCT GAGAACTTAC 
CCTGCTGCCA CCCGCATCGA CTCTTCCAAC CCGAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTCG TGGCACTCAA CTACCAGACC GATGATCTCC CTTTACATTT AAATGCTGCA 
ATGTTTGAGG CAAATGGTGG TTGTGGTTAT GTTTTGAAAC CTCCAGTTCT GTGGGACAAG 
AACTGCCCCA TGTATCAGAA GTTTTCTCCA CTAGAAAGAG ATCTGGACAG CATGGATCCT 
GCAGTCTATT CTTTAACTAT TGTCTCTGGT CAGAATGTGT GCCCCAGTAA TAGCATGGGA 
AGCCCGTGCA TTGAAGTCGA CGTCCTGGGC ATGCCTCTGG ACAGCTGCCA TTTCCGCACA 
AAGCCCATCC ATCGAAACAC CCTGAACCCC ATGTGGAACG AGCAGTTTCT GTTCCGCGTT 
CACTTCGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AAAACAATAG TTCAGCGGTA 
ACTGCTCAGA GAATCATTCC ACTGAAAGCT TTAAAACGAG GATATCGACA TCTTCAGCTG 
CGAAACCTTC ACAATGAAGT CTTGGAGATT TCTAGTTTAT TCATTAACAG CAGAAGGATG 
GAAGAAAATT CCTCTGGCAA TACCATGTCA GCCTCTTCGA TGTTTAATAC AGAAGAAAGA 
AAATGTTTGC AGACTCACAG AGTCACGGTG CATGGGGTCC CAGGGCCAGA GCCCTTTACC 
GTTTTCACTA TTAATGGAGG CACCAAGGCA AAGCAGCTTC TGCAGCAAAT TCTGACAAAT 
GAACAAGACA TCAAACCTGT TACCACAGAC TATTTTTTGA TGGAAGAAAA ATATTTTATA 
TCTAAAGAAA AGAATGAATG TAGGAAACAA CCATTCCAGA GAGCCATTGG TCCAGAAGAG 
GAGATCATGC AAATTTTAAG CAGCTGGTTT CCAGAAGAGG GATACGTGGG CAGGATTGTC 
TTAAAAACCC AGCAGGAAAA CCTAGAAGAG AAAAACATTG TTCAAGATGA CAAAGAGGTG 
ATCTTGAGCT CAGAGGAGGA GAGTTTCTTT GTCCAAGTGC ATGATGTTTC TCCAGAGCAA 
CCTCGAACAG TCATCAAAGC ACCCCGCGTC AGCACTGCAC AGGATGTCAT TCAGCAGACC 
TTATGCAAAG CCAAATATTC CTACAGCATC CTGAGCAACC CCAATCCAAG CGACTATGTG 
CTTTTGGAAG AGGTGGTGAA AGACACTACC AACAAGAAGA CCACCACACC AAAGTCCTCT 
CAGCGGGTCC TTCTGGATCA GGAGTGTGTG TTTCAAGCCC AAAGCAAGTG GAAAGGTGCA 
GGAAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CTCGAGAAGA TAAAAAGAAA 
GGCATTTCTT TCGCAAGTGA ACTCAAGAAG CTCACCAAGT CAACTAAACA GCCCCGAGGA 
CTTACATCAC CTTCTCAGCT CTTGACCTCA GAAAGTATCC AAACCAAGGA GGAGAAACCT 
GTGGGTGGCT TGTCCTCCAG TGACACAATG GATTACCGAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLCE1 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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