ROCK2 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK2
Entrez Gene ID	100969111
Full Name	Rho associated coiled-coil containing protein kinase 2
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003826972.2	XP_003827020.1	rho-associated protein kinase 2 isoform X1
XM_003826972.3	XP_003827020.1	rho-associated protein kinase 2 isoform X1
XM_008952314.2	XP_008950562.1	rho-associated protein kinase 2 isoform X2
XM_008952314.1	XP_008950562.1	rho-associated protein kinase 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ROCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa43673	XM_003826972.3 Latest version!	Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa43674	XM_008952314.2 Latest version!	Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OPa43673	XM_003826972.2	Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa43674	XM_008952314.1	Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK2 ( XM_003826972.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003827020.1 Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa43673
Clone ID Related Accession (Same CDS sequence)	XM_003826972.2 , XM_003826972.3
Accession Version	XM_003826972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1443456000000
Organism	Pan paniscus(pygmy chimpanzee)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014013809.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 2, 2014 this sequence version replaced XM_003826972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan paniscus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ACGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA AATGATTCAA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAGACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTATTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACTGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003827020.1
CDS	191..4357
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRENDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_003826972.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003826972.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ACGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA AATGATTCAA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAGACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTATTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACTGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_003826972.3 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003827020.1 Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa43673
Clone ID Related Accession (Same CDS sequence)	XM_003826972.2 , XM_003826972.3
Accession Version	XM_003826972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 1, 2018 this sequence version replaced XM_003826972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ACGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA AATGATTCAA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAGACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTATTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACTGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003827020.1
CDS	191..4357
Translation	MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRENDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_003826972.3
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003826972.3

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCAGGCG CGAGCCGCCA GAGGAAGCTG GAGGCGCTGA TCCGAGACCC TCGCTCCCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT AGATTTTCCT 
GCTTTGAGGA AAAACAAGAA CATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTCTACAGAT GAAGGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTGCA GTTGGTTCGT CACAAGGCAT CGCAGAAGGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCCTTTTT TTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAATAGCCCC TGGGTGGTTC AGCTTTTTTA TGCCTTTCAA 
GATGATAGGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAATT ACGATGTGCC TGAAAAATGG GCCAAATTTT ACACTGCTGA AGTTGTTCTT 
GCTCTGGATG CAATACACTC CATGGGTTTA ATACACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGACA TCTAAAATTA GCAGATTTTG GCACGTGTAT GAAGATGGAT 
GAAACAGGCA TGGTACATTG TGATACAGCA GTTGGAACAC CGGATTATAT ATCACCTGAG 
GTTCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTATGAGAT GCTAGTGGGG GATACTCCAT TTTATGCGGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAG AATTCACTGT GTTTCCCTGA AGATGCAGAA 
ATTTCCAAAC ATGCAAAGAA TCTCATCTGT GCTTTCTTAA CAGATAGGGA GGTACGACTT 
GGGAGAAATG GGGTGGAAGA AATCAGACAG CATCCTTTCT TTAAGAATGA TCAGTGGCAT 
TGGGATAACA TAAGAGAAAC GGCAGCTCCT GTAGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TCGATGACAT TGAAGATGAC AAAGGAGATG TAGAAACCTT CCCAATTCCT 
AAAGCTTTTG TTGGAAATCA GCTGCCTTTC ATCGGATTTA CCTACTATAG AGAAAATTTA 
TTATTAAGTG ACTCTCCATC TTGTAGAGAA AATGATTCAA TACAATCAAG GAAAAATGAA 
GAAAGTCAAG AGATTCAGAA AAAACTGTAT ACATTAGAAG AACATCTTAG CAATGAGATG 
CAAGCCAAAG AGGAACTGGA ACAGAAGTGC AAATCTGTTA ATACTCGCCT AGAAAAGACA 
GCAAAGGAGC TAGAAGAGGA GATTACCTTA CGGAAAAGTG TGGAATCAGC ATTAAGACAG 
TTAGAAAGAG AAAAGGCGCT TCTTCAGCAC AAAAATGCAG AATATCAGAG GAAAGCTGAT 
CATGAAGCAG ACAAAAAACG AAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATTTGA AAAAAAGAAA TCAAAACTCT CAAATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTA CTGCGAACAG AGTCTGATAC TGCAGCCCGG 
TTAAGGAAAA CCCAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGGAGGGATC GAACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATGTGGC CTAGAAGAAG ATTTAAAGAA CGGCAAAATC 
TTATTAGCGA AAGTAGAACT GGAGAAGAGA CAACTTCAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATACCAAC TAAAAGTTAT ACAGCAGAGC 
CTAGAACAAG AAGAAGCTGA ACATAAGGCC ACAAAGGCAC GACTAGCAGA TAAAAATAAG 
ATCTATGAGT CCATCGAAGA AGCCAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTC 
TTGGAGGAAA GAACTTTAAA ACAGAAAGTG GAGAACCTAT TGCTAGAAGC TGAGAAAAGA 
TGTTCTCTAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA GCTCCTTAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACCTGACAT TAAAAATAGA GCAAGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACCTG AAGATGCAAA CACAACAGGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAA AATAACCATC TCATGGAAAT GAAAATGAAC 
TTGGAAAAAC AAAATGCTGA ACTTCGAAAA GAACGTCAGG ATGCAGATGG GCAAATGAAA 
GAGCTCCAGG ATCAGCTCGA AGCAGAACAG TATTTCTCAA CCCTTTATAA AACACAAGTT 
AGGGAGCTTA AAGAAGAATG TGAAGAAAAG ACCAAACTTG GTAAAGAATT GCAGCAGAAG 
AAACAGGAAT TACAGGATGA ACGGGACTCT TTGGCTGCCC AACTGGAGAT CACCTTGACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAAG AACAATATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAGGAA 
CTTACTGAAA AAGATGCTAC AATTGCTTCT CTTGAGGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAATTGAA AGATGTTCAA 
GAGCAACTGT CAAGATTGAA AGATGAAGAA ATAAGCGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TATTAACAGA AAGAACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAG 
AAGGAGAATA GAAAGCTACA TATGGAGCTT AAATCTGAAC GTGAGAAATT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCAC AAATAGCTGA AGAGAGCCAG 
ATTCGAATTG AACTGCAGAT GACATTGGAC AGTAAAGACA GTGACATTGA GCAGCTGCGG 
TCACAACTCC AAGCCTTGCA TATTGGTCTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGG CAGATGATGG GTTTCCAGAA TCAAGATTAG AAGGATGGCT TTCATTGCCT 
GTACGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATTCTTT TCTATGACAG TGAACAAGAT AAAGAACAAT CCAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTGTA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTTGGA GAAAAATCTA ATTATATTTG CCACAAGGGA 
CATGAGTTTA TTCCTACTCT TTATCATTTC CCAACCAACT GTGAGGCTTG TATGAAGCCC 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT TTGGAGTGCC GCCGTTGCCA TATTAAGTGT 
CATAAAGATC ATATGGACAA AAAGGAGGAG ATTATAGCAC CTTGCAAAGT ATATTATGAT 
ATTTCAACGG CAAAGAATCT GTTATTACTA GCAAATTCTA CAGAAGAGCA GCAGAAGTGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCCCCAG CTCCAGACCC TTTTGCCCGA 
TCATCTCCTA GAACTTCAAT GAAGATACAG CAAAACCAGT CTATTAGACG GCCAAGTCGA 
CAGCTTGCCC CAAACAAACC TAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_008952314.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_008950562.1 Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OPa43674
Clone ID Related Accession (Same CDS sequence)	XM_008952314.2 , XM_008952314.1
Accession Version	XM_008952314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525017600000
Organism	Pan paniscus(pygmy chimpanzee)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 1, 2018 this sequence version replaced XM_008952314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCCTT TTTTTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAATAGC CCCTGGGTGG TTCAGCTTTT TTATGCCTTT 
CAAGATGATA GGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA CCTTGTAAAC 
CTTATGAGTA ATTACGATGT GCCTGAAAAA TGGGCCAAAT TTTACACTGC TGAAGTTGTT 
CTTGCTCTGG ATGCAATACA CTCCATGGGT TTAATACACA GAGATGTGAA GCCTGACAAC 
ATGCTCTTGG ATAAACATGG ACATCTAAAA TTAGCAGATT TTGGCACGTG TATGAAGATG 
GATGAAACAG GCATGGTACA TTGTGATACA GCAGTTGGAA CACCGGATTA TATATCACCT 
GAGGTTCTGA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTATGA GATGCTAGTG GGGGATACTC CATTTTATGC GGATTCACTT 
GTAGGAACAT ATAGCAAAAT TATGGATCAT AAGAATTCAC TGTGTTTCCC TGAAGATGCA 
GAAATTTCCA AACATGCAAA GAATCTCATC TGTGCTTTCT TAACAGATAG GGAGGTACGA 
CTTGGGAGAA ATGGGGTGGA AGAAATCAGA CAGCATCCTT TCTTTAAGAA TGATCAGTGG 
CATTGGGATA ACATAAGAGA AACGGCAGCT CCTGTAGTAC CTGAACTCAG CAGTGACATA 
GACAGCAGCA ATTTCGATGA CATTGAAGAT GACAAAGGAG ATGTAGAAAC CTTCCCAATT 
CCTAAAGCTT TTGTTGGAAA TCAGCTGCCT TTCATCGGAT TTACCTACTA TAGAGAAAAT 
TTATTATTAA GTGACTCTCC ATCTTGTAGA GAAAATGATT CAATACAATC AAGGAAAAAT 
GAAGAAAGTC AAGAGATTCA GAAAAAACTG TATACATTAG AAGAACATCT TAGCAATGAG 
ATGCAAGCCA AAGAGGAACT GGAACAGAAG TGCAAATCTG TTAATACTCG CCTAGAAAAG 
ACAGCAAAGG AGCTAGAAGA GGAGATTACC TTACGGAAAA GTGTGGAATC AGCATTAAGA 
CAGTTAGAAA GAGAAAAGGC GCTTCTTCAG CACAAAAATG CAGAATATCA GAGGAAAGCT 
GATCATGAAG CAGACAAAAA ACGAAATTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATT TGAAAAAAAG AAATCAAAAC TCTCAAATAT CCACTGAGAA AGTGAATCAA 
CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGCGAA CAGAGTCTGA TACTGCAGCC 
CGGTTAAGGA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTG GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TCTAGAATCT GAAAGGAGGG ATCGAACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATGT GGCCTAGAAG AAGATTTAAA GAACGGCAAA 
ATCTTATTAG CGAAAGTAGA ACTGGAGAAG AGACAACTTC AGGAGAGATT TACTGATTTG 
GAAAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATACC AACTAAAAGT TATACAGCAG 
AGCCTAGAAC AAGAAGAAGC TGAACATAAG GCCACAAAGG CACGACTAGC AGATAAAAAT 
AAGATCTATG AGTCCATCGA AGAAGCCAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTCTTGGAGG AAAGAACTTT AAAACAGAAA GTGGAGAACC TATTGCTAGA AGCTGAGAAA 
AGATGTTCTC TATTAGACTG TGACCTCAAA CAGTCACAGC AGAAAATAAA TGAGCTCCTT 
AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACCTGA CATTAAAAAT AGAGCAAGAA 
ACTCAGAAGC GCTGCCTTAC ACAAAATGAC CTGAAGATGC AAACACAACA GGTTAACACA 
CTAAAAATGT CAGAAAAGCA GTTAAAGCAA GAAAATAACC ATCTCATGGA AATGAAAATG 
AACTTGGAAA AACAAAATGC TGAACTTCGA AAAGAACGTC AGGATGCAGA TGGGCAAATG 
AAAGAGCTCC AGGATCAGCT CGAAGCAGAA CAGTATTTCT CAACCCTTTA TAAAACACAA 
GTTAGGGAGC TTAAAGAAGA ATGTGAAGAA AAGACCAAAC TTGGTAAAGA ATTGCAGCAG 
AAGAAACAGG AATTACAGGA TGAACGGGAC TCTTTGGCTG CCCAACTGGA GATCACCTTG 
ACCAAAGCAG ATTCTGAGCA ACTGGCTCGT TCAATTGCTG AAGAACAATA TTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG 
GAACTTACTG AAAAAGATGC TACAATTGCT TCTCTTGAGG AAACTAATAG GACACTAACT 
AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGTT 
CAAGAGCAAC TGTCAAGATT GAAAGATGAA GAAATAAGCG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTATTAAC AGAAAGAACA CTCAAAACTC AAGCTGTGAA TAAGTTGGCT 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAATG ACACAGATGT GCGGAGAAAA 
GAGAAGGAGA ATAGAAAGCT ACATATGGAG CTTAAATCTG AACGTGAGAA ATTGACCCAG 
CAGATGATCA AGTATCAGAA AGAACTGAAT GAAATGCAGG CACAAATAGC TGAAGAGAGC 
CAGATTCGAA TTGAACTGCA GATGACATTG GACAGTAAAG ACAGTGACAT TGAGCAGCTG 
CGGTCACAAC TCCAAGCCTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCA 
GGGGATGCTG AGGCAGATGA TGGGTTTCCA GAATCAAGAT TAGAAGGATG GCTTTCATTG 
CCTGTACGAA ACAACACTAA GAAATTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGT 
AAGAAGATTC TTTTCTATGA CAGTGAACAA GATAAAGAAC AATCCAATCC TTACATGGTT 
TTAGATATAG ACAAGTTATT TCATGTCCGA CCAGTTACAC AGACAGATGT GTATAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTCCAG ATTCTGTATG CCAATGAAGG AGAAAGTAAG 
AAGGAACAAG AATTTCCAGT GGAGCCAGTT GGAGAAAAAT CTAATTATAT TTGCCACAAG 
GGACATGAGT TTATTCCTAC TCTTTATCAT TTCCCAACCA ACTGTGAGGC TTGTATGAAG 
CCCCTGTGGC ACATGTTTAA GCCTCCTCCT GCTTTGGAGT GCCGCCGTTG CCATATTAAG 
TGTCATAAAG ATCATATGGA CAAAAAGGAG GAGATTATAG CACCTTGCAA AGTATATTAT 
GATATTTCAA CGGCAAAGAA TCTGTTATTA CTAGCAAATT CTACAGAAGA GCAGCAGAAG 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCCC CAGCTCCAGA CCCTTTTGCC 
CGATCATCTC CTAGAACTTC AATGAAGATA CAGCAAAACC AGTCTATTAG ACGGCCAAGT 
CGACAGCTTG CCCCAAACAA ACCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008950562.1
CDS	43..3852
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRENDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_008952314.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008952314.2

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCCTT TTTTTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAATAGC CCCTGGGTGG TTCAGCTTTT TTATGCCTTT 
CAAGATGATA GGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA CCTTGTAAAC 
CTTATGAGTA ATTACGATGT GCCTGAAAAA TGGGCCAAAT TTTACACTGC TGAAGTTGTT 
CTTGCTCTGG ATGCAATACA CTCCATGGGT TTAATACACA GAGATGTGAA GCCTGACAAC 
ATGCTCTTGG ATAAACATGG ACATCTAAAA TTAGCAGATT TTGGCACGTG TATGAAGATG 
GATGAAACAG GCATGGTACA TTGTGATACA GCAGTTGGAA CACCGGATTA TATATCACCT 
GAGGTTCTGA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTATGA GATGCTAGTG GGGGATACTC CATTTTATGC GGATTCACTT 
GTAGGAACAT ATAGCAAAAT TATGGATCAT AAGAATTCAC TGTGTTTCCC TGAAGATGCA 
GAAATTTCCA AACATGCAAA GAATCTCATC TGTGCTTTCT TAACAGATAG GGAGGTACGA 
CTTGGGAGAA ATGGGGTGGA AGAAATCAGA CAGCATCCTT TCTTTAAGAA TGATCAGTGG 
CATTGGGATA ACATAAGAGA AACGGCAGCT CCTGTAGTAC CTGAACTCAG CAGTGACATA 
GACAGCAGCA ATTTCGATGA CATTGAAGAT GACAAAGGAG ATGTAGAAAC CTTCCCAATT 
CCTAAAGCTT TTGTTGGAAA TCAGCTGCCT TTCATCGGAT TTACCTACTA TAGAGAAAAT 
TTATTATTAA GTGACTCTCC ATCTTGTAGA GAAAATGATT CAATACAATC AAGGAAAAAT 
GAAGAAAGTC AAGAGATTCA GAAAAAACTG TATACATTAG AAGAACATCT TAGCAATGAG 
ATGCAAGCCA AAGAGGAACT GGAACAGAAG TGCAAATCTG TTAATACTCG CCTAGAAAAG 
ACAGCAAAGG AGCTAGAAGA GGAGATTACC TTACGGAAAA GTGTGGAATC AGCATTAAGA 
CAGTTAGAAA GAGAAAAGGC GCTTCTTCAG CACAAAAATG CAGAATATCA GAGGAAAGCT 
GATCATGAAG CAGACAAAAA ACGAAATTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATT TGAAAAAAAG AAATCAAAAC TCTCAAATAT CCACTGAGAA AGTGAATCAA 
CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGCGAA CAGAGTCTGA TACTGCAGCC 
CGGTTAAGGA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTG GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TCTAGAATCT GAAAGGAGGG ATCGAACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATGT GGCCTAGAAG AAGATTTAAA GAACGGCAAA 
ATCTTATTAG CGAAAGTAGA ACTGGAGAAG AGACAACTTC AGGAGAGATT TACTGATTTG 
GAAAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATACC AACTAAAAGT TATACAGCAG 
AGCCTAGAAC AAGAAGAAGC TGAACATAAG GCCACAAAGG CACGACTAGC AGATAAAAAT 
AAGATCTATG AGTCCATCGA AGAAGCCAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTCTTGGAGG AAAGAACTTT AAAACAGAAA GTGGAGAACC TATTGCTAGA AGCTGAGAAA 
AGATGTTCTC TATTAGACTG TGACCTCAAA CAGTCACAGC AGAAAATAAA TGAGCTCCTT 
AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACCTGA CATTAAAAAT AGAGCAAGAA 
ACTCAGAAGC GCTGCCTTAC ACAAAATGAC CTGAAGATGC AAACACAACA GGTTAACACA 
CTAAAAATGT CAGAAAAGCA GTTAAAGCAA GAAAATAACC ATCTCATGGA AATGAAAATG 
AACTTGGAAA AACAAAATGC TGAACTTCGA AAAGAACGTC AGGATGCAGA TGGGCAAATG 
AAAGAGCTCC AGGATCAGCT CGAAGCAGAA CAGTATTTCT CAACCCTTTA TAAAACACAA 
GTTAGGGAGC TTAAAGAAGA ATGTGAAGAA AAGACCAAAC TTGGTAAAGA ATTGCAGCAG 
AAGAAACAGG AATTACAGGA TGAACGGGAC TCTTTGGCTG CCCAACTGGA GATCACCTTG 
ACCAAAGCAG ATTCTGAGCA ACTGGCTCGT TCAATTGCTG AAGAACAATA TTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG 
GAACTTACTG AAAAAGATGC TACAATTGCT TCTCTTGAGG AAACTAATAG GACACTAACT 
AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGTT 
CAAGAGCAAC TGTCAAGATT GAAAGATGAA GAAATAAGCG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTATTAAC AGAAAGAACA CTCAAAACTC AAGCTGTGAA TAAGTTGGCT 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAATG ACACAGATGT GCGGAGAAAA 
GAGAAGGAGA ATAGAAAGCT ACATATGGAG CTTAAATCTG AACGTGAGAA ATTGACCCAG 
CAGATGATCA AGTATCAGAA AGAACTGAAT GAAATGCAGG CACAAATAGC TGAAGAGAGC 
CAGATTCGAA TTGAACTGCA GATGACATTG GACAGTAAAG ACAGTGACAT TGAGCAGCTG 
CGGTCACAAC TCCAAGCCTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCA 
GGGGATGCTG AGGCAGATGA TGGGTTTCCA GAATCAAGAT TAGAAGGATG GCTTTCATTG 
CCTGTACGAA ACAACACTAA GAAATTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGT 
AAGAAGATTC TTTTCTATGA CAGTGAACAA GATAAAGAAC AATCCAATCC TTACATGGTT 
TTAGATATAG ACAAGTTATT TCATGTCCGA CCAGTTACAC AGACAGATGT GTATAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTCCAG ATTCTGTATG CCAATGAAGG AGAAAGTAAG 
AAGGAACAAG AATTTCCAGT GGAGCCAGTT GGAGAAAAAT CTAATTATAT TTGCCACAAG 
GGACATGAGT TTATTCCTAC TCTTTATCAT TTCCCAACCA ACTGTGAGGC TTGTATGAAG 
CCCCTGTGGC ACATGTTTAA GCCTCCTCCT GCTTTGGAGT GCCGCCGTTG CCATATTAAG 
TGTCATAAAG ATCATATGGA CAAAAAGGAG GAGATTATAG CACCTTGCAA AGTATATTAT 
GATATTTCAA CGGCAAAGAA TCTGTTATTA CTAGCAAATT CTACAGAAGA GCAGCAGAAG 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCCC CAGCTCCAGA CCCTTTTGCC 
CGATCATCTC CTAGAACTTC AATGAAGATA CAGCAAAACC AGTCTATTAG ACGGCCAAGT 
CGACAGCTTG CCCCAAACAA ACCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_008952314.1 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_008950562.1 Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OPa43674
Clone ID Related Accession (Same CDS sequence)	XM_008952314.2 , XM_008952314.1
Accession Version	XM_008952314.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1443456000000
Organism	Pan paniscus(pygmy chimpanzee)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014013809.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan paniscus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCCTT TTTTTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAATAGC CCCTGGGTGG TTCAGCTTTT TTATGCCTTT 
CAAGATGATA GGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA CCTTGTAAAC 
CTTATGAGTA ATTACGATGT GCCTGAAAAA TGGGCCAAAT TTTACACTGC TGAAGTTGTT 
CTTGCTCTGG ATGCAATACA CTCCATGGGT TTAATACACA GAGATGTGAA GCCTGACAAC 
ATGCTCTTGG ATAAACATGG ACATCTAAAA TTAGCAGATT TTGGCACGTG TATGAAGATG 
GATGAAACAG GCATGGTACA TTGTGATACA GCAGTTGGAA CACCGGATTA TATATCACCT 
GAGGTTCTGA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTATGA GATGCTAGTG GGGGATACTC CATTTTATGC GGATTCACTT 
GTAGGAACAT ATAGCAAAAT TATGGATCAT AAGAATTCAC TGTGTTTCCC TGAAGATGCA 
GAAATTTCCA AACATGCAAA GAATCTCATC TGTGCTTTCT TAACAGATAG GGAGGTACGA 
CTTGGGAGAA ATGGGGTGGA AGAAATCAGA CAGCATCCTT TCTTTAAGAA TGATCAGTGG 
CATTGGGATA ACATAAGAGA AACGGCAGCT CCTGTAGTAC CTGAACTCAG CAGTGACATA 
GACAGCAGCA ATTTCGATGA CATTGAAGAT GACAAAGGAG ATGTAGAAAC CTTCCCAATT 
CCTAAAGCTT TTGTTGGAAA TCAGCTGCCT TTCATCGGAT TTACCTACTA TAGAGAAAAT 
TTATTATTAA GTGACTCTCC ATCTTGTAGA GAAAATGATT CAATACAATC AAGGAAAAAT 
GAAGAAAGTC AAGAGATTCA GAAAAAACTG TATACATTAG AAGAACATCT TAGCAATGAG 
ATGCAAGCCA AAGAGGAACT GGAACAGAAG TGCAAATCTG TTAATACTCG CCTAGAAAAG 
ACAGCAAAGG AGCTAGAAGA GGAGATTACC TTACGGAAAA GTGTGGAATC AGCATTAAGA 
CAGTTAGAAA GAGAAAAGGC GCTTCTTCAG CACAAAAATG CAGAATATCA GAGGAAAGCT 
GATCATGAAG CAGACAAAAA ACGAAATTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATT TGAAAAAAAG AAATCAAAAC TCTCAAATAT CCACTGAGAA AGTGAATCAA 
CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGCGAA CAGAGTCTGA TACTGCAGCC 
CGGTTAAGGA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTG GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TCTAGAATCT GAAAGGAGGG ATCGAACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATGT GGCCTAGAAG AAGATTTAAA GAACGGCAAA 
ATCTTATTAG CGAAAGTAGA ACTGGAGAAG AGACAACTTC AGGAGAGATT TACTGATTTG 
GAAAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATACC AACTAAAAGT TATACAGCAG 
AGCCTAGAAC AAGAAGAAGC TGAACATAAG GCCACAAAGG CACGACTAGC AGATAAAAAT 
AAGATCTATG AGTCCATCGA AGAAGCCAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTCTTGGAGG AAAGAACTTT AAAACAGAAA GTGGAGAACC TATTGCTAGA AGCTGAGAAA 
AGATGTTCTC TATTAGACTG TGACCTCAAA CAGTCACAGC AGAAAATAAA TGAGCTCCTT 
AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACCTGA CATTAAAAAT AGAGCAAGAA 
ACTCAGAAGC GCTGCCTTAC ACAAAATGAC CTGAAGATGC AAACACAACA GGTTAACACA 
CTAAAAATGT CAGAAAAGCA GTTAAAGCAA GAAAATAACC ATCTCATGGA AATGAAAATG 
AACTTGGAAA AACAAAATGC TGAACTTCGA AAAGAACGTC AGGATGCAGA TGGGCAAATG 
AAAGAGCTCC AGGATCAGCT CGAAGCAGAA CAGTATTTCT CAACCCTTTA TAAAACACAA 
GTTAGGGAGC TTAAAGAAGA ATGTGAAGAA AAGACCAAAC TTGGTAAAGA ATTGCAGCAG 
AAGAAACAGG AATTACAGGA TGAACGGGAC TCTTTGGCTG CCCAACTGGA GATCACCTTG 
ACCAAAGCAG ATTCTGAGCA ACTGGCTCGT TCAATTGCTG AAGAACAATA TTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG 
GAACTTACTG AAAAAGATGC TACAATTGCT TCTCTTGAGG AAACTAATAG GACACTAACT 
AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGTT 
CAAGAGCAAC TGTCAAGATT GAAAGATGAA GAAATAAGCG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTATTAAC AGAAAGAACA CTCAAAACTC AAGCTGTGAA TAAGTTGGCT 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAATG ACACAGATGT GCGGAGAAAA 
GAGAAGGAGA ATAGAAAGCT ACATATGGAG CTTAAATCTG AACGTGAGAA ATTGACCCAG 
CAGATGATCA AGTATCAGAA AGAACTGAAT GAAATGCAGG CACAAATAGC TGAAGAGAGC 
CAGATTCGAA TTGAACTGCA GATGACATTG GACAGTAAAG ACAGTGACAT TGAGCAGCTG 
CGGTCACAAC TCCAAGCCTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCA 
GGGGATGCTG AGGCAGATGA TGGGTTTCCA GAATCAAGAT TAGAAGGATG GCTTTCATTG 
CCTGTACGAA ACAACACTAA GAAATTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGT 
AAGAAGATTC TTTTCTATGA CAGTGAACAA GATAAAGAAC AATCCAATCC TTACATGGTT 
TTAGATATAG ACAAGTTATT TCATGTCCGA CCAGTTACAC AGACAGATGT GTATAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTCCAG ATTCTGTATG CCAATGAAGG AGAAAGTAAG 
AAGGAACAAG AATTTCCAGT GGAGCCAGTT GGAGAAAAAT CTAATTATAT TTGCCACAAG 
GGACATGAGT TTATTCCTAC TCTTTATCAT TTCCCAACCA ACTGTGAGGC TTGTATGAAG 
CCCCTGTGGC ACATGTTTAA GCCTCCTCCT GCTTTGGAGT GCCGCCGTTG CCATATTAAG 
TGTCATAAAG ATCATATGGA CAAAAAGGAG GAGATTATAG CACCTTGCAA AGTATATTAT 
GATATTTCAA CGGCAAAGAA TCTGTTATTA CTAGCAAATT CTACAGAAGA GCAGCAGAAG 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCCC CAGCTCCAGA CCCTTTTGCC 
CGATCATCTC CTAGAACTTC AATGAAGATA CAGCAAAACC AGTCTATTAG ACGGCCAAGT 
CGACAGCTTG CCCCAAACAA ACCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008950562.1
CDS	43..3852
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRENDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_008952314.1
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008952314.1

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCCTT TTTTTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAATAGC CCCTGGGTGG TTCAGCTTTT TTATGCCTTT 
CAAGATGATA GGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA CCTTGTAAAC 
CTTATGAGTA ATTACGATGT GCCTGAAAAA TGGGCCAAAT TTTACACTGC TGAAGTTGTT 
CTTGCTCTGG ATGCAATACA CTCCATGGGT TTAATACACA GAGATGTGAA GCCTGACAAC 
ATGCTCTTGG ATAAACATGG ACATCTAAAA TTAGCAGATT TTGGCACGTG TATGAAGATG 
GATGAAACAG GCATGGTACA TTGTGATACA GCAGTTGGAA CACCGGATTA TATATCACCT 
GAGGTTCTGA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTATGA GATGCTAGTG GGGGATACTC CATTTTATGC GGATTCACTT 
GTAGGAACAT ATAGCAAAAT TATGGATCAT AAGAATTCAC TGTGTTTCCC TGAAGATGCA 
GAAATTTCCA AACATGCAAA GAATCTCATC TGTGCTTTCT TAACAGATAG GGAGGTACGA 
CTTGGGAGAA ATGGGGTGGA AGAAATCAGA CAGCATCCTT TCTTTAAGAA TGATCAGTGG 
CATTGGGATA ACATAAGAGA AACGGCAGCT CCTGTAGTAC CTGAACTCAG CAGTGACATA 
GACAGCAGCA ATTTCGATGA CATTGAAGAT GACAAAGGAG ATGTAGAAAC CTTCCCAATT 
CCTAAAGCTT TTGTTGGAAA TCAGCTGCCT TTCATCGGAT TTACCTACTA TAGAGAAAAT 
TTATTATTAA GTGACTCTCC ATCTTGTAGA GAAAATGATT CAATACAATC AAGGAAAAAT 
GAAGAAAGTC AAGAGATTCA GAAAAAACTG TATACATTAG AAGAACATCT TAGCAATGAG 
ATGCAAGCCA AAGAGGAACT GGAACAGAAG TGCAAATCTG TTAATACTCG CCTAGAAAAG 
ACAGCAAAGG AGCTAGAAGA GGAGATTACC TTACGGAAAA GTGTGGAATC AGCATTAAGA 
CAGTTAGAAA GAGAAAAGGC GCTTCTTCAG CACAAAAATG CAGAATATCA GAGGAAAGCT 
GATCATGAAG CAGACAAAAA ACGAAATTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATT TGAAAAAAAG AAATCAAAAC TCTCAAATAT CCACTGAGAA AGTGAATCAA 
CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGCGAA CAGAGTCTGA TACTGCAGCC 
CGGTTAAGGA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTG GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TCTAGAATCT GAAAGGAGGG ATCGAACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATGT GGCCTAGAAG AAGATTTAAA GAACGGCAAA 
ATCTTATTAG CGAAAGTAGA ACTGGAGAAG AGACAACTTC AGGAGAGATT TACTGATTTG 
GAAAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATACC AACTAAAAGT TATACAGCAG 
AGCCTAGAAC AAGAAGAAGC TGAACATAAG GCCACAAAGG CACGACTAGC AGATAAAAAT 
AAGATCTATG AGTCCATCGA AGAAGCCAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTCTTGGAGG AAAGAACTTT AAAACAGAAA GTGGAGAACC TATTGCTAGA AGCTGAGAAA 
AGATGTTCTC TATTAGACTG TGACCTCAAA CAGTCACAGC AGAAAATAAA TGAGCTCCTT 
AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACCTGA CATTAAAAAT AGAGCAAGAA 
ACTCAGAAGC GCTGCCTTAC ACAAAATGAC CTGAAGATGC AAACACAACA GGTTAACACA 
CTAAAAATGT CAGAAAAGCA GTTAAAGCAA GAAAATAACC ATCTCATGGA AATGAAAATG 
AACTTGGAAA AACAAAATGC TGAACTTCGA AAAGAACGTC AGGATGCAGA TGGGCAAATG 
AAAGAGCTCC AGGATCAGCT CGAAGCAGAA CAGTATTTCT CAACCCTTTA TAAAACACAA 
GTTAGGGAGC TTAAAGAAGA ATGTGAAGAA AAGACCAAAC TTGGTAAAGA ATTGCAGCAG 
AAGAAACAGG AATTACAGGA TGAACGGGAC TCTTTGGCTG CCCAACTGGA GATCACCTTG 
ACCAAAGCAG ATTCTGAGCA ACTGGCTCGT TCAATTGCTG AAGAACAATA TTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG 
GAACTTACTG AAAAAGATGC TACAATTGCT TCTCTTGAGG AAACTAATAG GACACTAACT 
AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGTT 
CAAGAGCAAC TGTCAAGATT GAAAGATGAA GAAATAAGCG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTATTAAC AGAAAGAACA CTCAAAACTC AAGCTGTGAA TAAGTTGGCT 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAATG ACACAGATGT GCGGAGAAAA 
GAGAAGGAGA ATAGAAAGCT ACATATGGAG CTTAAATCTG AACGTGAGAA ATTGACCCAG 
CAGATGATCA AGTATCAGAA AGAACTGAAT GAAATGCAGG CACAAATAGC TGAAGAGAGC 
CAGATTCGAA TTGAACTGCA GATGACATTG GACAGTAAAG ACAGTGACAT TGAGCAGCTG 
CGGTCACAAC TCCAAGCCTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCA 
GGGGATGCTG AGGCAGATGA TGGGTTTCCA GAATCAAGAT TAGAAGGATG GCTTTCATTG 
CCTGTACGAA ACAACACTAA GAAATTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGT 
AAGAAGATTC TTTTCTATGA CAGTGAACAA GATAAAGAAC AATCCAATCC TTACATGGTT 
TTAGATATAG ACAAGTTATT TCATGTCCGA CCAGTTACAC AGACAGATGT GTATAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTCCAG ATTCTGTATG CCAATGAAGG AGAAAGTAAG 
AAGGAACAAG AATTTCCAGT GGAGCCAGTT GGAGAAAAAT CTAATTATAT TTGCCACAAG 
GGACATGAGT TTATTCCTAC TCTTTATCAT TTCCCAACCA ACTGTGAGGC TTGTATGAAG 
CCCCTGTGGC ACATGTTTAA GCCTCCTCCT GCTTTGGAGT GCCGCCGTTG CCATATTAAG 
TGTCATAAAG ATCATATGGA CAAAAAGGAG GAGATTATAG CACCTTGCAA AGTATATTAT 
GATATTTCAA CGGCAAAGAA TCTGTTATTA CTAGCAAATT CTACAGAAGA GCAGCAGAAG 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCCC CAGCTCCAGA CCCTTTTGCC 
CGATCATCTC CTAGAACTTC AATGAAGATA CAGCAAAACC AGTCTATTAG ACGGCCAAGT 
CGACAGCTTG CCCCAAACAA ACCTAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ROCK2 cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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