USP25 cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP25
Entrez Gene ID	100964520
Full Name	ubiquitin specific peptidase 25
Gene Type	protein-coding
Organism	Otolemur garnettii(small-eared galago)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012801589.2	XP_012657043.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_023509677.1	XP_023365445.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_012801589.1	XP_012657043.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_012801587.1	XP_012657041.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_012801587.2	XP_012657041.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_012801588.1	XP_012657042.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2

Pathways from BioSystems
Gene Ontology

The following USP25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOt03540	XM_012801587.2 Latest version!	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOt03541	XM_012801588.1 Latest version!	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOt03542	XM_012801589.2 Latest version!	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOt26430	XM_023509677.1 Latest version!	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OOt03542	XM_012801589.1	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOt03540	XM_012801587.1	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP25 ( XM_012801587.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012657041.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOt03540
Clone ID Related Accession (Same CDS sequence)	XM_012801587.1 , XM_012801587.2
Accession Version	XM_012801587.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433952000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Otolemur garnettii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GAAACCTGAA 
AATCCTGCAA CTCAGCCACC TTCTAACCCG CGAGTTGTGG AGGTGGAGAT TCCTCACGTA 
GGGAAATTTA TGATTGAATC AAAGGAGGGG GGGTATGATG ATGAGATCAT GATGACACCG 
AACATGCAAG GTATTATCAT GGCGATAGGT CAATCCAGGA ATGTATATGA CAGGTGTGGT 
CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGGTTGGT TAAGCTGGCC 
CAAGAAGATA CCCCTCCAGA AACAGATTAC CGTTTACATC ATGTAGTTGT CTACTTTATC 
CAGAACCAGG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGCAGATAGA 
AATTTGAGTT TTGATGAAAG GTGTCACAAC ATAATGAAAG TTGCTCAAGC CAAACTGGAA 
ATGATCAAAC CTGAAGAAGT GAACTTGGAG GAGTATAAGG AGTGGCATCA GGATTATAGG 
AAATTCAGGG AAACGACTAT GTATCTCATA ATCGGGCTAG AAAACTTCCA GAGAGAAAGT 
TATATAGATT CTTTGCTGTT CCTTATCTGT GCGTATCAGA ATAATAAAGA ACTCTTGTCC 
AAAGGCTTAT ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA 
CTAAAATTAA ACGAGCAGGC TGCAGAACTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC 
AATGGTCTGA TTATCATGAA TGAGTTTATT GTCCCATTTT TGCCCTTATT ACTGGTGGAT 
GAGATGGAAG AGAAAGATAT ACTAGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAC 
CTTGGTCAAG AAATGGAGTC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCCAAACTG 
CTTGATTGTT CTATGGAGAT CAAGAGTTTC CACGAGCCAC CGAAGTTACC TCCCTACTCC 
ACGCATGAAC TCTGTGAGCG ATTTGCCCGA ATCATGTTGT CCCTCAGTCG GACTCCTGCT 
GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012657041.1
CDS	368..3739
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSKPENPATQPPSNPRVVEVEIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGQSRNVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_012801587.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012801587.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GAAACCTGAA 
AATCCTGCAA CTCAGCCACC TTCTAACCCG CGAGTTGTGG AGGTGGAGAT TCCTCACGTA 
GGGAAATTTA TGATTGAATC AAAGGAGGGG GGGTATGATG ATGAGATCAT GATGACACCG 
AACATGCAAG GTATTATCAT GGCGATAGGT CAATCCAGGA ATGTATATGA CAGGTGTGGT 
CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGGTTGGT TAAGCTGGCC 
CAAGAAGATA CCCCTCCAGA AACAGATTAC CGTTTACATC ATGTAGTTGT CTACTTTATC 
CAGAACCAGG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGCAGATAGA 
AATTTGAGTT TTGATGAAAG GTGTCACAAC ATAATGAAAG TTGCTCAAGC CAAACTGGAA 
ATGATCAAAC CTGAAGAAGT GAACTTGGAG GAGTATAAGG AGTGGCATCA GGATTATAGG 
AAATTCAGGG AAACGACTAT GTATCTCATA ATCGGGCTAG AAAACTTCCA GAGAGAAAGT 
TATATAGATT CTTTGCTGTT CCTTATCTGT GCGTATCAGA ATAATAAAGA ACTCTTGTCC 
AAAGGCTTAT ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA 
CTAAAATTAA ACGAGCAGGC TGCAGAACTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC 
AATGGTCTGA TTATCATGAA TGAGTTTATT GTCCCATTTT TGCCCTTATT ACTGGTGGAT 
GAGATGGAAG AGAAAGATAT ACTAGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAC 
CTTGGTCAAG AAATGGAGTC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCCAAACTG 
CTTGATTGTT CTATGGAGAT CAAGAGTTTC CACGAGCCAC CGAAGTTACC TCCCTACTCC 
ACGCATGAAC TCTGTGAGCG ATTTGCCCGA ATCATGTTGT CCCTCAGTCG GACTCCTGCT 
GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_012801587.2 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012657041.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOt03540
Clone ID Related Accession (Same CDS sequence)	XM_012801587.1 , XM_012801587.2
Accession Version	XM_012801587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515600000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 12, 2018 this sequence version replaced XM_012801587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Otolemur garnettii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GAAACCTGAA 
AATCCTGCAA CTCAGCCACC TTCTAACCCG CGAGTTGTGG AGGTGGAGAT TCCTCACGTA 
GGGAAATTTA TGATTGAATC AAAGGAGGGG GGGTATGATG ATGAGATCAT GATGACACCG 
AACATGCAAG GTATTATCAT GGCGATAGGT CAATCCAGGA ATGTATATGA CAGGTGTGGT 
CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGGTTGGT TAAGCTGGCC 
CAAGAAGATA CCCCTCCAGA AACAGATTAC CGTTTACATC ATGTAGTTGT CTACTTTATC 
CAGAACCAGG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGCAGATAGA 
AATTTGAGTT TTGATGAAAG GTGTCACAAC ATAATGAAAG TTGCTCAAGC CAAACTGGAA 
ATGATCAAAC CTGAAGAAGT GAACTTGGAG GAGTATAAGG AGTGGCATCA GGATTATAGG 
AAATTCAGGG AAACGACTAT GTATCTCATA ATCGGGCTAG AAAACTTCCA GAGAGAAAGT 
TATATAGATT CTTTGCTGTT CCTTATCTGT GCGTATCAGA ATAATAAAGA ACTCTTGTCC 
AAAGGCTTAT ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA 
CTAAAATTAA ACGAGCAGGC TGCAGAACTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC 
AATGGTCTGA TTATCATGAA TGAGTTTATT GTCCCATTTT TGCCCTTATT ACTGGTGGAT 
GAGATGGAAG AGAAAGATAT ACTAGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAC 
CTTGGTCAAG AAATGGAGTC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCCAAACTG 
CTTGATTGTT CTATGGAGAT CAAGAGTTTC CACGAGCCAC CGAAGTTACC TCCCTACTCC 
ACGCATGAAC TCTGTGAGCG ATTTGCCCGA ATCATGTTGT CCCTCAGTCG GACTCCTGCT 
GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012657041.1
CDS	373..3744
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSKPENPATQPPSNPRVVEVEIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGQSRNVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_012801587.2
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012801587.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GAAACCTGAA 
AATCCTGCAA CTCAGCCACC TTCTAACCCG CGAGTTGTGG AGGTGGAGAT TCCTCACGTA 
GGGAAATTTA TGATTGAATC AAAGGAGGGG GGGTATGATG ATGAGATCAT GATGACACCG 
AACATGCAAG GTATTATCAT GGCGATAGGT CAATCCAGGA ATGTATATGA CAGGTGTGGT 
CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGGTTGGT TAAGCTGGCC 
CAAGAAGATA CCCCTCCAGA AACAGATTAC CGTTTACATC ATGTAGTTGT CTACTTTATC 
CAGAACCAGG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGCAGATAGA 
AATTTGAGTT TTGATGAAAG GTGTCACAAC ATAATGAAAG TTGCTCAAGC CAAACTGGAA 
ATGATCAAAC CTGAAGAAGT GAACTTGGAG GAGTATAAGG AGTGGCATCA GGATTATAGG 
AAATTCAGGG AAACGACTAT GTATCTCATA ATCGGGCTAG AAAACTTCCA GAGAGAAAGT 
TATATAGATT CTTTGCTGTT CCTTATCTGT GCGTATCAGA ATAATAAAGA ACTCTTGTCC 
AAAGGCTTAT ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA 
CTAAAATTAA ACGAGCAGGC TGCAGAACTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC 
AATGGTCTGA TTATCATGAA TGAGTTTATT GTCCCATTTT TGCCCTTATT ACTGGTGGAT 
GAGATGGAAG AGAAAGATAT ACTAGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAC 
CTTGGTCAAG AAATGGAGTC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCCAAACTG 
CTTGATTGTT CTATGGAGAT CAAGAGTTTC CACGAGCCAC CGAAGTTACC TCCCTACTCC 
ACGCATGAAC TCTGTGAGCG ATTTGCCCGA ATCATGTTGT CCCTCAGTCG GACTCCTGCT 
GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_012801588.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012657042.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOt03541
Clone ID Related Accession (Same CDS sequence)	XM_012801588.1
Accession Version	XM_012801588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433952000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Otolemur garnettii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTCAAT CCAGGAATGT ATATGACAGG 
TGTGGTCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AGTATTCAAG GTTGGTTAAG 
CTGGCCCAAG AAGATACCCC TCCAGAAACA GATTACCGTT TACATCATGT AGTTGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTGCTAGA ACAATTTGCA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAGGAGT ATAAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC GACTATGTAT CTCATAATCG GGCTAGAAAA CTTCCAGAGA 
GAAAGTTATA TAGATTCTTT GCTGTTCCTT ATCTGTGCGT ATCAGAATAA TAAAGAACTC 
TTGTCCAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTACTAA AATTAAACGA GCAGGCTGCA GAACTGTTTG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTCTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC CTTATTACTG 
GTGGATGAGA TGGAAGAGAA AGATATACTA GCTGTGGAAG ACATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAGTCAAAC CTCCAAGAGA AGCTGACAGA TTTTCTGCCC 
AAACTGCTTG ATTGTTCTAT GGAGATCAAG AGTTTCCACG AGCCACCGAA GTTACCTCCC 
TACTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGGACT 
CCTGCTGATG GAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012657042.1
CDS	368..3625
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGQSRNVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_012801588.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012801588.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTCAAT CCAGGAATGT ATATGACAGG 
TGTGGTCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AGTATTCAAG GTTGGTTAAG 
CTGGCCCAAG AAGATACCCC TCCAGAAACA GATTACCGTT TACATCATGT AGTTGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATC ATTGAGAAAA CATTGCTAGA ACAATTTGCA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TCAAACCTGA AGAAGTGAAC TTGGAGGAGT ATAAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC GACTATGTAT CTCATAATCG GGCTAGAAAA CTTCCAGAGA 
GAAAGTTATA TAGATTCTTT GCTGTTCCTT ATCTGTGCGT ATCAGAATAA TAAAGAACTC 
TTGTCCAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTACTAA AATTAAACGA GCAGGCTGCA GAACTGTTTG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTCTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC CTTATTACTG 
GTGGATGAGA TGGAAGAGAA AGATATACTA GCTGTGGAAG ACATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAGTCAAAC CTCCAAGAGA AGCTGACAGA TTTTCTGCCC 
AAACTGCTTG ATTGTTCTAT GGAGATCAAG AGTTTCCACG AGCCACCGAA GTTACCTCCC 
TACTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGGACT 
CCTGCTGATG GAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_012801589.2 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012657043.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOt03542
Clone ID Related Accession (Same CDS sequence)	XM_012801589.2 , XM_012801589.1
Accession Version	XM_012801589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515600000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 12, 2018 this sequence version replaced XM_012801589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Otolemur garnettii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GGCAATTAAG 
TTGGAGTATT CAAGGTTGGT TAAGCTGGCC CAAGAAGATA CCCCTCCAGA AACAGATTAC 
CGTTTACATC ATGTAGTTGT CTACTTTATC CAGAACCAGG CACCAAAGAA AATCATTGAG 
AAAACATTGC TAGAACAATT TGCAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAC 
ATAATGAAAG TTGCTCAAGC CAAACTGGAA ATGATCAAAC CTGAAGAAGT GAACTTGGAG 
GAGTATAAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACGACTAT GTATCTCATA 
ATCGGGCTAG AAAACTTCCA GAGAGAAAGT TATATAGATT CTTTGCTGTT CCTTATCTGT 
GCGTATCAGA ATAATAAAGA ACTCTTGTCC AAAGGCTTAT ACAGAGGACA TGATGAAGAA 
TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ACGAGCAGGC TGCAGAACTG 
TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA TGAGTTTATT 
GTCCCATTTT TGCCCTTATT ACTGGTGGAT GAGATGGAAG AGAAAGATAT ACTAGCTGTG 
GAAGACATGA GAAATCGATG GTGTTCCTAC CTTGGTCAAG AAATGGAGTC AAACCTCCAA 
GAGAAGCTGA CAGATTTTCT GCCCAAACTG CTTGATTGTT CTATGGAGAT CAAGAGTTTC 
CACGAGCCAC CGAAGTTACC TCCCTACTCC ACGCATGAAC TCTGTGAGCG ATTTGCCCGA 
ATCATGTTGT CCCTCAGTCG GACTCCTGCT GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012657043.1
CDS	464..3625
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_012801589.2
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012801589.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GGCAATTAAG 
TTGGAGTATT CAAGGTTGGT TAAGCTGGCC CAAGAAGATA CCCCTCCAGA AACAGATTAC 
CGTTTACATC ATGTAGTTGT CTACTTTATC CAGAACCAGG CACCAAAGAA AATCATTGAG 
AAAACATTGC TAGAACAATT TGCAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAC 
ATAATGAAAG TTGCTCAAGC CAAACTGGAA ATGATCAAAC CTGAAGAAGT GAACTTGGAG 
GAGTATAAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACGACTAT GTATCTCATA 
ATCGGGCTAG AAAACTTCCA GAGAGAAAGT TATATAGATT CTTTGCTGTT CCTTATCTGT 
GCGTATCAGA ATAATAAAGA ACTCTTGTCC AAAGGCTTAT ACAGAGGACA TGATGAAGAA 
TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ACGAGCAGGC TGCAGAACTG 
TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA TGAGTTTATT 
GTCCCATTTT TGCCCTTATT ACTGGTGGAT GAGATGGAAG AGAAAGATAT ACTAGCTGTG 
GAAGACATGA GAAATCGATG GTGTTCCTAC CTTGGTCAAG AAATGGAGTC AAACCTCCAA 
GAGAAGCTGA CAGATTTTCT GCCCAAACTG CTTGATTGTT CTATGGAGAT CAAGAGTTTC 
CACGAGCCAC CGAAGTTACC TCCCTACTCC ACGCATGAAC TCTGTGAGCG ATTTGCCCGA 
ATCATGTTGT CCCTCAGTCG GACTCCTGCT GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_012801589.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_012657043.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

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CloneID	OOt03542
Clone ID Related Accession (Same CDS sequence)	XM_012801589.2 , XM_012801589.1
Accession Version	XM_012801589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433952000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Otolemur garnettii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GGCAATTAAG 
TTGGAGTATT CAAGGTTGGT TAAGCTGGCC CAAGAAGATA CCCCTCCAGA AACAGATTAC 
CGTTTACATC ATGTAGTTGT CTACTTTATC CAGAACCAGG CACCAAAGAA AATCATTGAG 
AAAACATTGC TAGAACAATT TGCAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAC 
ATAATGAAAG TTGCTCAAGC CAAACTGGAA ATGATCAAAC CTGAAGAAGT GAACTTGGAG 
GAGTATAAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACGACTAT GTATCTCATA 
ATCGGGCTAG AAAACTTCCA GAGAGAAAGT TATATAGATT CTTTGCTGTT CCTTATCTGT 
GCGTATCAGA ATAATAAAGA ACTCTTGTCC AAAGGCTTAT ACAGAGGACA TGATGAAGAA 
TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ACGAGCAGGC TGCAGAACTG 
TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA TGAGTTTATT 
GTCCCATTTT TGCCCTTATT ACTGGTGGAT GAGATGGAAG AGAAAGATAT ACTAGCTGTG 
GAAGACATGA GAAATCGATG GTGTTCCTAC CTTGGTCAAG AAATGGAGTC AAACCTCCAA 
GAGAAGCTGA CAGATTTTCT GCCCAAACTG CTTGATTGTT CTATGGAGAT CAAGAGTTTC 
CACGAGCCAC CGAAGTTACC TCCCTACTCC ACGCATGAAC TCTGTGAGCG ATTTGCCCGA 
ATCATGTTGT CCCTCAGTCG GACTCCTGCT GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012657043.1
CDS	368..3529
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_012801589.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012801589.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA GCAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT 
AAAGCGTGTT TGAAGAGGAC CCCTACGGAA GTTTGGAGGG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTCGGG CTAAAGAATG TTGGCAATAC CTGCTGGTTT 
AGTGCTGTGA TTCAGTCATT GTTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTGTTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCGGTTGA AATTCTTAAG GATGCTTTCA AGTCAAATGA CTCACAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA CTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TCGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT CGAAGCTGCA 
ATGATCGAAG GAGAAATTGA GTCTTTACAT TCAGAGAACT CGGGGAAATC AGGCCAGGAG 
CACTGGTTCA CTGAATTACC ACCTGTGTTA ACGTTTGAAT TGTCAAGGTT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTGG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG GAACAGAGAA ATTACAAGAA TTAAAAGGGA AGAGATCAAA 
AGGCTTAAAG ATTACCTCAC AGTGTTACAA CAAAGACTAG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTCCC CTTGGTCGAT GTTCTGCAGT ATGCCTTGGA GTTCGCCTCC 
AGCAAACCCG TTTGCACCTC CCCTGTGGAC GATATCGGTG CCAGTTCCAC TGCTAGTAGC 
TCTGGACCAT CACAGACGTT ACCAAGCACA ACAGAACAGC AGGGAGCTCC TTCCGAACTG 
CCCAGCACAT CACCGGCATC AGTCACTGCC ATTTCATCGA GATCGGTGAT ACACAAACCC 
TTCACTCAGT CCCGAATACC TCCTGATCTG CCCATGCATC CAGCACCGAG GCACATAACA 
GAAGAAGAAC TTTCTGTGCT GGAGAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAC 
TCTGACAAAT CTATGATCCA AGTCCCTTAT CGCTTACATG CTGTTTTGGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCGTAC ATTTTTGATC ATCACGAAAG CAGGTGGATG 
AAGTACAATG ATATTGCTGT GACGAAATCA TCCTGGGAGG AACTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCGTACTGT CTAATGTACA TAAATGATAA GGCACAATTC 
TTAATACAAG AGGAGTTTAA TAAAGAAACT GGACAGGCTC TTGTTGGAAT AGAAACCTTA 
CCACCTGATT TGAGAGATTT TGTTGAGGAA GACAACAAAC GATTTGAAAA AGAACTAGAA 
GAGTGGGACG CACAACTCGC CCAGAAGGCC TTGCAGGAAA AGCTTTTAGC AGCTCAGAAA 
TTGAGGGAGT CAGAGTCTTC TGGGATAACA GCACAAGCAG GAGAACCAGA ATACCTAGAG 
CAGCCATCAA GAAGTGATTT CTCAAAGCAC TTGAAGGAAG ACACTATTCG AGTAATTACC 
AAGGCTTCAC ATGAGCATGA AGATAAAAGT CCTGAAACAG TTTTACAGTC GGCAATTAAG 
TTGGAGTATT CAAGGTTGGT TAAGCTGGCC CAAGAAGATA CCCCTCCAGA AACAGATTAC 
CGTTTACATC ATGTAGTTGT CTACTTTATC CAGAACCAGG CACCAAAGAA AATCATTGAG 
AAAACATTGC TAGAACAATT TGCAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAC 
ATAATGAAAG TTGCTCAAGC CAAACTGGAA ATGATCAAAC CTGAAGAAGT GAACTTGGAG 
GAGTATAAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACGACTAT GTATCTCATA 
ATCGGGCTAG AAAACTTCCA GAGAGAAAGT TATATAGATT CTTTGCTGTT CCTTATCTGT 
GCGTATCAGA ATAATAAAGA ACTCTTGTCC AAAGGCTTAT ACAGAGGACA TGATGAAGAA 
TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ACGAGCAGGC TGCAGAACTG 
TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA TGAGTTTATT 
GTCCCATTTT TGCCCTTATT ACTGGTGGAT GAGATGGAAG AGAAAGATAT ACTAGCTGTG 
GAAGACATGA GAAATCGATG GTGTTCCTAC CTTGGTCAAG AAATGGAGTC AAACCTCCAA 
GAGAAGCTGA CAGATTTTCT GCCCAAACTG CTTGATTGTT CTATGGAGAT CAAGAGTTTC 
CACGAGCCAC CGAAGTTACC TCCCTACTCC ACGCATGAAC TCTGTGAGCG ATTTGCCCGA 
ATCATGTTGT CCCTCAGTCG GACTCCTGCT GATGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_023509677.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_023365445.1 Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

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CloneID	OOt26430
Clone ID Related Accession (Same CDS sequence)	XM_023509677.1
Accession Version	XM_023509677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515600000000
Organism	Otolemur garnettii(small-eared galago)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852404.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Otolemur garnettii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAGA CAAACACAGT ACATGCCTAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAATATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAGCAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AACAAGCCAT TAGCAGAGTT 
CTTGAAGCCA GCATAGCAGA AAATAAAGCG TGTTTGAAGA GGACCCCTAC GGAAGTTTGG 
AGGGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT CGGGCTAAAG 
AATGTTGGCA ATACCTGCTG GTTTAGTGCT GTGATTCAGT CATTGTTCAA TCTTTTGGAA 
TTTAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG CTCAAGATTT ACCCCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGCTGA GGTATCTGTT TGCACTTCTT 
GTTGGTACCA AAAGGAAGTA TGTTGATCCA TCAAGAGCGG TTGAAATTCT TAAGGATGCT 
TTCAAGTCAA ATGACTCACA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTACTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAGA CGGATGAAGA GAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTCGCTG TGGGAGTACT TGAAGGTAAA 
AAATTTGAAA ACACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG GTTCAAAGAT 
CTGCATGAGT GCCTCGAAGC TGCAATGATC GAAGGAGAAA TTGAGTCTTT ACATTCAGAG 
AACTCGGGGA AATCAGGCCA GGAGCACTGG TTCACTGAAT TACCACCTGT GTTAACGTTT 
GAATTGTCAA GGTTTGAATT TAATCAGGCA TTGGGAAGAC CAGAAAAAAT TCACAACAAA 
TTGGAATTTC CCCAAGTTTT ATATTTGGAC AGATACATGC ACAGGAACAG AGAAATTACA 
AGAATTAAAA GGGAAGAGAT CAAAAGGCTT AAAGATTACC TCACAGTGTT ACAACAAAGA 
CTAGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TCCCCTTGGT CGATGTTCTG 
CAGTATGCCT TGGAGTTCGC CTCCAGCAAA CCCGTTTGCA CCTCCCCTGT GGACGATATC 
GGTGCCAGTT CCACTGCTAG TAGCTCTGGA CCATCACAGA CGTTACCAAG CACAACAGAA 
CAGCAGGGAG CTCCTTCCGA ACTGCCCAGC ACATCACCGG CATCAGTCAC TGCCATTTCA 
TCGAGATCGG TGATACACAA ACCCTTCACT CAGTCCCGAA TACCTCCTGA TCTGCCCATG 
CATCCAGCAC CGAGGCACAT AACAGAAGAA GAACTTTCTG TGCTGGAGAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTGC AGGAAAGCAT ATCCAGAATC 
CATCGAACAA TAGAACTAAT GTACTCTGAC AAATCTATGA TCCAAGTCCC TTATCGCTTA 
CATGCTGTTT TGGTTCACGA AGGCCAAGCT AATGCTGGGC ACTACTGGGC GTACATTTTT 
GATCATCACG AAAGCAGGTG GATGAAGTAC AATGATATTG CTGTGACGAA ATCATCCTGG 
GAGGAACTAG TGAGGGACTC TTTTGGTGGT TATAGAAATG CCAGTGCGTA CTGTCTAATG 
TACATAAATG ATAAGGCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGACAG 
GCTCTTGTTG GAATAGAAAC CTTACCACCT GATTTGAGAG ATTTTGTTGA GGAAGACAAC 
AAACGATTTG AAAAAGAACT AGAAGAGTGG GACGCACAAC TCGCCCAGAA GGCCTTGCAG 
GAAAAGCTTT TAGCAGCTCA GAAATTGAGG GAGTCAGAGT CTTCTGGGAT AACAGCACAA 
GCAGGAGAAC CAGAATACCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAG 
GAAGACACTA TTCGAGTAAT TACCAAGGCT TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTAC AGTCGGCAAT TAAGTTGGAG TATTCAAGGT TGGTTAAGCT GGCCCAAGAA 
GATACCCCTC CAGAAACAGA TTACCGTTTA CATCATGTAG TTGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATCAT TGAGAAAACA TTGCTAGAAC AATTTGCAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATC 
AAACCTGAAG AAGTGAACTT GGAGGAGTAT AAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACGA CTATGTATCT CATAATCGGG CTAGAAAACT TCCAGAGAGA AAGTTATATA 
GATTCTTTGC TGTTCCTTAT CTGTGCGTAT CAGAATAATA AAGAACTCTT GTCCAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTACTAAAA 
TTAAACGAGC AGGCTGCAGA ACTGTTTGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
CTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCCT TATTACTGGT GGATGAGATG 
GAAGAGAAAG ATATACTAGC TGTGGAAGAC ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AGTCAAACCT CCAAGAGAAG CTGACAGATT TTCTGCCCAA ACTGCTTGAT 
TGTTCTATGG AGATCAAGAG TTTCCACGAG CCACCGAAGT TACCTCCCTA CTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGGACTCC TGCTGATGGA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023365445.1
CDS	373..3618
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRQTQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESSRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIGASSTASSSGPSQTLPSTTEQQGAPSELPSTSPASVTAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNKRFEKELEEWDAQLAQKALQEKLLAAQKLRESESSGITAQAGEPEYLEQPSRSDFSKHLKEDTIRVITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYKEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSMEIKSFHEPPKLPPYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_023509677.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023509677.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAATGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAGC TGAGAGAAAT CACCGGCATT AATGACACCC AGATACTGCA GCAAGCCCTG 
AAGGATAGTA ATGGAAACCT GGAGTTAGCA GTGGCTTTTC TTACTGCGAA GAATGCCAAG 
ACCCCTCAGC AAGAGGAGAC AACATACTAC CAAACAGCAC TTCCCGGCAA CGACAGATAC 
ATCAGCGTGG GGAGCCAAGC AGACACAAGA CAAACACAGT ACATGCCTAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAATATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTCACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAGCAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AACAAGCCAT TAGCAGAGTT 
CTTGAAGCCA GCATAGCAGA AAATAAAGCG TGTTTGAAGA GGACCCCTAC GGAAGTTTGG 
AGGGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT CGGGCTAAAG 
AATGTTGGCA ATACCTGCTG GTTTAGTGCT GTGATTCAGT CATTGTTCAA TCTTTTGGAA 
TTTAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG CTCAAGATTT ACCCCGAAAC 
CAAAAGGAAC ATCGGAATTT GCCTTTTATG CGTGAGCTGA GGTATCTGTT TGCACTTCTT 
GTTGGTACCA AAAGGAAGTA TGTTGATCCA TCAAGAGCGG TTGAAATTCT TAAGGATGCT 
TTCAAGTCAA ATGACTCACA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTACTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAGGAGA CGGATGAAGA GAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTCGCTG TGGGAGTACT TGAAGGTAAA 
AAATTTGAAA ACACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG GTTCAAAGAT 
CTGCATGAGT GCCTCGAAGC TGCAATGATC GAAGGAGAAA TTGAGTCTTT ACATTCAGAG 
AACTCGGGGA AATCAGGCCA GGAGCACTGG TTCACTGAAT TACCACCTGT GTTAACGTTT 
GAATTGTCAA GGTTTGAATT TAATCAGGCA TTGGGAAGAC CAGAAAAAAT TCACAACAAA 
TTGGAATTTC CCCAAGTTTT ATATTTGGAC AGATACATGC ACAGGAACAG AGAAATTACA 
AGAATTAAAA GGGAAGAGAT CAAAAGGCTT AAAGATTACC TCACAGTGTT ACAACAAAGA 
CTAGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TCCCCTTGGT CGATGTTCTG 
CAGTATGCCT TGGAGTTCGC CTCCAGCAAA CCCGTTTGCA CCTCCCCTGT GGACGATATC 
GGTGCCAGTT CCACTGCTAG TAGCTCTGGA CCATCACAGA CGTTACCAAG CACAACAGAA 
CAGCAGGGAG CTCCTTCCGA ACTGCCCAGC ACATCACCGG CATCAGTCAC TGCCATTTCA 
TCGAGATCGG TGATACACAA ACCCTTCACT CAGTCCCGAA TACCTCCTGA TCTGCCCATG 
CATCCAGCAC CGAGGCACAT AACAGAAGAA GAACTTTCTG TGCTGGAGAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTGC AGGAAAGCAT ATCCAGAATC 
CATCGAACAA TAGAACTAAT GTACTCTGAC AAATCTATGA TCCAAGTCCC TTATCGCTTA 
CATGCTGTTT TGGTTCACGA AGGCCAAGCT AATGCTGGGC ACTACTGGGC GTACATTTTT 
GATCATCACG AAAGCAGGTG GATGAAGTAC AATGATATTG CTGTGACGAA ATCATCCTGG 
GAGGAACTAG TGAGGGACTC TTTTGGTGGT TATAGAAATG CCAGTGCGTA CTGTCTAATG 
TACATAAATG ATAAGGCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGACAG 
GCTCTTGTTG GAATAGAAAC CTTACCACCT GATTTGAGAG ATTTTGTTGA GGAAGACAAC 
AAACGATTTG AAAAAGAACT AGAAGAGTGG GACGCACAAC TCGCCCAGAA GGCCTTGCAG 
GAAAAGCTTT TAGCAGCTCA GAAATTGAGG GAGTCAGAGT CTTCTGGGAT AACAGCACAA 
GCAGGAGAAC CAGAATACCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAG 
GAAGACACTA TTCGAGTAAT TACCAAGGCT TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTAC AGTCGGCAAT TAAGTTGGAG TATTCAAGGT TGGTTAAGCT GGCCCAAGAA 
GATACCCCTC CAGAAACAGA TTACCGTTTA CATCATGTAG TTGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATCAT TGAGAAAACA TTGCTAGAAC AATTTGCAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATC 
AAACCTGAAG AAGTGAACTT GGAGGAGTAT AAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACGA CTATGTATCT CATAATCGGG CTAGAAAACT TCCAGAGAGA AAGTTATATA 
GATTCTTTGC TGTTCCTTAT CTGTGCGTAT CAGAATAATA AAGAACTCTT GTCCAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTACTAAAA 
TTAAACGAGC AGGCTGCAGA ACTGTTTGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
CTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCCT TATTACTGGT GGATGAGATG 
GAAGAGAAAG ATATACTAGC TGTGGAAGAC ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AGTCAAACCT CCAAGAGAAG CTGACAGATT TTCTGCCCAA ACTGCTTGAT 
TGTTCTATGG AGATCAAGAG TTTCCACGAG CCACCGAAGT TACCTCCCTA CTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGGACTCC TGCTGATGGA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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USP25 cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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