nup214 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup214
Entrez Gene ID	101170858
Full Name	nucleoporin 214
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011481722.3	XP_011480024.1	nuclear pore complex protein Nup214 isoform X1
XM_011481723.2	XP_011480025.1	nuclear pore complex protein Nup214 isoform X3
XM_011481722.2	XP_011480024.1	nuclear pore complex protein Nup214 isoform X1
XM_011481723.3	XP_011480025.1	nuclear pore complex protein Nup214 isoform X3
XM_011481725.2	XP_011480027.1	nuclear pore complex protein Nup214 isoform X4
XM_011481725.3	XP_011480027.1	nuclear pore complex protein Nup214 isoform X4
XM_011481724.2	XP_011480026.1	nuclear pore complex protein Nup214 isoform X2
XM_011481724.3	XP_011480026.1	nuclear pore complex protein Nup214 isoform X2

Pathways from BioSystems
Gene Ontology

The following nup214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa107474	XM_011481722.3 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa107475	XM_011481724.3 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa107476	XM_011481723.3 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa107477	XM_011481725.3 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa33580	XM_011481722.2 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa33581	XM_011481723.2 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa33582	XM_011481724.2 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa33583	XM_011481725.2 Latest version!	Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup214 ( XM_011481722.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480024.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa107474
Clone ID Related Accession (Same CDS sequence)	XM_011481722.3
Accession Version	XM_011481722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011481722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCGGGTG CTCCGTCGTT TGGCAGCTGG AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480024.1
CDS	121..6099
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481722.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481722.3

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCGGGTG CTCCGTCGTT TGGCAGCTGG AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481724.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480026.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.

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CloneID	OJa107475
Clone ID Related Accession (Same CDS sequence)	XM_011481724.3
Accession Version	XM_011481724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011481724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGT CAAGGTTTCT 
CCTCTTAAAC AGTCCCAACT GCGCAACTTC CTGTCAAAAG GGAAAATGCC TCCTGTGCGT 
TCAACTGCAC CAGCCAACTT GTCGCGCTCG GCCTTCCTGG CCCCTAAAAA CCTTGAGGAC 
TTGGATGATG TGAGCTCAAC GTCCTCCTTA TCGCAGTCTA TAGAGCTTCT CCCATCTCAC 
TTGGAGTTGG AGGAAGAGGA AGAACTACAG CCCCTGCAGC CTTCACCTAT GAATTCCATG 
ACCCAATCAC TTCCCACCCC ACGTCACCCC ACTGTTGTTC GAACGCCCTC CATTCAGCCA 
GGCTTTGGAG CTATACAGTC CACCCCTCTA ACAAAGATGC ACCCTGTTCC TGGTATGGGC 
TTTGTGCTAA GCCCCATTAT CAACCCTGTT CCTACAAATA GGATTAACCT CAGCGGGGCT 
GAAAGTACTG CGCTTGCCAC AAAGACGGTA AAGCATGGAG CACCACCTGC AGAGCGGAGC 
ATCCCTGTGA CCATCTCTGC CCAGCAGGCT GCTGCCACCG CGGCTCTGCG GAGACAAATG 
GCCAATCAGA AGACGGCTGT TGTCAACACT TCTTTGACAG AGTCCACCTT AAAAACTGTC 
CCTCAAGTAG TGAATGTTCA GGAGCTTAAG GACAAAGGGG CACCTATGCC AGTGTCCTCT 
ATGGTCAGCT CCTCGGCGCC AGATCCCTCA GTCCAGGGCT TCACAGCAGT TTCTAGCAGC 
CAAGTCAAAC GAAACACACC GGGTCTGCAA AAGATACCCA CAGAGAATGC ATCTATTCCA 
CAGACAGGCT TTGTCTTTGG CCAACCATCC AAAACAGACC TTTCCGGGGG TCCACCTAGT 
TCACTTGAGC AAAACACCAG CAAGGCCTTC TCATTCACCT CAGGATCGAC GGGCTTCAGT 
TTTTCATCTG TAACACAAGG TGCTGGATTG TCTCAAGGGA AGGATGTGAA CAAGTTCTCC 
ATTGGCGGAA ATGGCAAAGG GTTGTTTGGC CAAACTGGAG AGGAGCCATT CTCCCTTCCC 
ACTAAGTCAA ACTCTCCTGG TCTGAGCAAT GGGTCTTCAA GCTCATCGGC CAACAGTTTA 
GGGGAAGTAG CAAAACAGGG TTCCACTGTT ACTGGTTCTA AGACAGAGCC GCAGCCTCTA 
AAGGCAGCTG GAGGGGAGAC ATTAGGCAGT TTTTCAGGAC TACGAGTGGG ACACGGAGAA 
GAAGCAAACG AATCCTCCAC AAAGACCACT GCTCCTGCAT TTTCCTTCGG GAATGCTGGA 
CTTGGCCATA GCAAGTCAGC TCCTCAGTTT AACTTTGGTT CAACTCCCCT TAAGTCTGCC 
GAAGACGGGG CTGCAGCAGA CATGCCTAAG GTGTCAGGAT CAGGCAGCTT GTTTAAACCT 
CCTGATTCAA ACTCCATGCC AACCTTTTCT ATAACCCCTT CAACTACAGC TGCTTCAGCG 
CTGCCTACAT CTTTAAGTAG TCTTCTTGCA GCTCCTACTG AACAGCCTAA AGCTACTCAG 
CAACCTTCAG AAAGCACATC GGCTGCTTTA CCAGAACTGA GTACCAAGGA ACTTGCTGTA 
GACCTGGCTA GTGTTCCGGC AGAACCTGAG CCTCTAGTGA CTGCAGTTGC CACAACAACC 
ACGCCAACAT CTGCAGCACC AACACTTCCT ATGGTCACTC CTGTCCCTAC TGCAGACTCC 
TCTTCTACAG TTCCTGCAGA GACGGAAACA ACTCTGCCTC CAGCACCACC AACTGCTTCT 
TCCACAGAAG AAGCCACCCC AGACCCCTCT TCTACACCTG CCGTCTCCAC ATCTACCACC 
GCTGTCCCAC TTACCATCGA GGTAACCTCA GCAGCTTTAC CAGCTTCCTC CTCAGAAAAA 
CCAGGCTCCA TTTTTACCTC TGCTACTCCT GCAACCACCA CTAGCAATTC TGTTGGGGCC 
ATACCAGCCA TTAACACAGC TGCTCCCACA ACCACTACCG CTGCAGTTAT CACCAGTGCA 
GCAACGACAG CTGCTAATGT GTTTGGGCAG CCTGCCGCTC CTCCTGCCTC TTCTGCCCCT 
GCAACTACCG GATTCGGCTC ATCTGGATTT GGCGCACCAA CAGAAACCGG TTTCGGCAAA 
TCTGTATTTG GCGTGGGTGG GTTTGGACAG CCTGCTAGCA TAGGAGGAAC TTCGAGTGGC 
TTTTCATTCA CCCAGTCAGC ATTCGGAGCA AGTTCAAGCA GTGCGACCAC TGGAGGAGGT 
GGTCTTTTCG GTGCTCCCAC TGTTACCAAT GCAAGCTCCT TCTCCTTCGG CACCAGCAGT 
TCCAGCACAC CCAGCAGCAC CGGGCTGTTT AGCCAAAGCA CCCCGTCAGT ATTCGGCCAG 
AGCTCTGGAT TTGGGCAAGG ATCTATGTTC AGAAATAACA CCACCACATC ATCTGCGTCA 
GGCTTCAGCT TTGGACAGCC CTCAGGTTTT GGCTGTTCGG CGGCCACACC TGTGTTTGGC 
CAACAATCCA CCACTGGGAG CATATTTGGA CAGCAACAGC AACCTTCAAC TGGTGGAGGT 
TTGTTCGGTC AAAGTTCGAC CGGCGCTGCA ACCTCGTCCT CTGGCGGATT CTTCAGCGGG 
TTGGGAGGCA AACCCAGTGA AGACGCTGCC AACAAGAACC CATTTGGTAC CACCGCCTCC 
ACTGGCGTGT TTGGCCAGAG CACGCAGTCT GGGCCCAACA GCCTCTTTGG GAGCAGTAGC 
GCCAAGACCT TTGGATTTGG TCAGCCCTCC TTCGGGGAGC AGAAGCCCAG TGGGACCTTC 
AGTACTGGTG GAGGCAGTGT TGCATGTCAG GGCTTTGGTT CCTTCTCCAC ACCATCTAAA 
TCAACGAGCG GCTTTGGCAG CGCCCCCGTG TTCGGAAGCC CCCCTTCTTT TGGCGGTTCT 
CCTGCATTCG GTGGCGGCGC TTCATTCAGT TCAAGTCCGT CTTTCAGCAA CCCTTTAGCT 
TCCCAGTCCA GTAAAGTGTT TGGCGAGGGA ACAGCAGCCG CCAGCATGGG AGGATTTGGT 
TTCTCCTCAC CCCCCAGTGC CCCCTCCTTT GGGACTCTCG CCACTCAGAC TGCGCCGTCA 
TTCGGGAGCT TGGCCCAGCA GGGCCCTGGA TTTGGAAGCC AGCCCAGCAG CTTCTCAGGC 
TTTGGCCAGC AGCCACAGAC GGGAGGATTT TCAGGAAACG CATTTGGATC TCCAAATCCA 
CCGGGTGCTC CGTCGTTTGG CAGCTGGAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480026.1
CDS	121..6096
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481724.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481724.3

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGT CAAGGTTTCT 
CCTCTTAAAC AGTCCCAACT GCGCAACTTC CTGTCAAAAG GGAAAATGCC TCCTGTGCGT 
TCAACTGCAC CAGCCAACTT GTCGCGCTCG GCCTTCCTGG CCCCTAAAAA CCTTGAGGAC 
TTGGATGATG TGAGCTCAAC GTCCTCCTTA TCGCAGTCTA TAGAGCTTCT CCCATCTCAC 
TTGGAGTTGG AGGAAGAGGA AGAACTACAG CCCCTGCAGC CTTCACCTAT GAATTCCATG 
ACCCAATCAC TTCCCACCCC ACGTCACCCC ACTGTTGTTC GAACGCCCTC CATTCAGCCA 
GGCTTTGGAG CTATACAGTC CACCCCTCTA ACAAAGATGC ACCCTGTTCC TGGTATGGGC 
TTTGTGCTAA GCCCCATTAT CAACCCTGTT CCTACAAATA GGATTAACCT CAGCGGGGCT 
GAAAGTACTG CGCTTGCCAC AAAGACGGTA AAGCATGGAG CACCACCTGC AGAGCGGAGC 
ATCCCTGTGA CCATCTCTGC CCAGCAGGCT GCTGCCACCG CGGCTCTGCG GAGACAAATG 
GCCAATCAGA AGACGGCTGT TGTCAACACT TCTTTGACAG AGTCCACCTT AAAAACTGTC 
CCTCAAGTAG TGAATGTTCA GGAGCTTAAG GACAAAGGGG CACCTATGCC AGTGTCCTCT 
ATGGTCAGCT CCTCGGCGCC AGATCCCTCA GTCCAGGGCT TCACAGCAGT TTCTAGCAGC 
CAAGTCAAAC GAAACACACC GGGTCTGCAA AAGATACCCA CAGAGAATGC ATCTATTCCA 
CAGACAGGCT TTGTCTTTGG CCAACCATCC AAAACAGACC TTTCCGGGGG TCCACCTAGT 
TCACTTGAGC AAAACACCAG CAAGGCCTTC TCATTCACCT CAGGATCGAC GGGCTTCAGT 
TTTTCATCTG TAACACAAGG TGCTGGATTG TCTCAAGGGA AGGATGTGAA CAAGTTCTCC 
ATTGGCGGAA ATGGCAAAGG GTTGTTTGGC CAAACTGGAG AGGAGCCATT CTCCCTTCCC 
ACTAAGTCAA ACTCTCCTGG TCTGAGCAAT GGGTCTTCAA GCTCATCGGC CAACAGTTTA 
GGGGAAGTAG CAAAACAGGG TTCCACTGTT ACTGGTTCTA AGACAGAGCC GCAGCCTCTA 
AAGGCAGCTG GAGGGGAGAC ATTAGGCAGT TTTTCAGGAC TACGAGTGGG ACACGGAGAA 
GAAGCAAACG AATCCTCCAC AAAGACCACT GCTCCTGCAT TTTCCTTCGG GAATGCTGGA 
CTTGGCCATA GCAAGTCAGC TCCTCAGTTT AACTTTGGTT CAACTCCCCT TAAGTCTGCC 
GAAGACGGGG CTGCAGCAGA CATGCCTAAG GTGTCAGGAT CAGGCAGCTT GTTTAAACCT 
CCTGATTCAA ACTCCATGCC AACCTTTTCT ATAACCCCTT CAACTACAGC TGCTTCAGCG 
CTGCCTACAT CTTTAAGTAG TCTTCTTGCA GCTCCTACTG AACAGCCTAA AGCTACTCAG 
CAACCTTCAG AAAGCACATC GGCTGCTTTA CCAGAACTGA GTACCAAGGA ACTTGCTGTA 
GACCTGGCTA GTGTTCCGGC AGAACCTGAG CCTCTAGTGA CTGCAGTTGC CACAACAACC 
ACGCCAACAT CTGCAGCACC AACACTTCCT ATGGTCACTC CTGTCCCTAC TGCAGACTCC 
TCTTCTACAG TTCCTGCAGA GACGGAAACA ACTCTGCCTC CAGCACCACC AACTGCTTCT 
TCCACAGAAG AAGCCACCCC AGACCCCTCT TCTACACCTG CCGTCTCCAC ATCTACCACC 
GCTGTCCCAC TTACCATCGA GGTAACCTCA GCAGCTTTAC CAGCTTCCTC CTCAGAAAAA 
CCAGGCTCCA TTTTTACCTC TGCTACTCCT GCAACCACCA CTAGCAATTC TGTTGGGGCC 
ATACCAGCCA TTAACACAGC TGCTCCCACA ACCACTACCG CTGCAGTTAT CACCAGTGCA 
GCAACGACAG CTGCTAATGT GTTTGGGCAG CCTGCCGCTC CTCCTGCCTC TTCTGCCCCT 
GCAACTACCG GATTCGGCTC ATCTGGATTT GGCGCACCAA CAGAAACCGG TTTCGGCAAA 
TCTGTATTTG GCGTGGGTGG GTTTGGACAG CCTGCTAGCA TAGGAGGAAC TTCGAGTGGC 
TTTTCATTCA CCCAGTCAGC ATTCGGAGCA AGTTCAAGCA GTGCGACCAC TGGAGGAGGT 
GGTCTTTTCG GTGCTCCCAC TGTTACCAAT GCAAGCTCCT TCTCCTTCGG CACCAGCAGT 
TCCAGCACAC CCAGCAGCAC CGGGCTGTTT AGCCAAAGCA CCCCGTCAGT ATTCGGCCAG 
AGCTCTGGAT TTGGGCAAGG ATCTATGTTC AGAAATAACA CCACCACATC ATCTGCGTCA 
GGCTTCAGCT TTGGACAGCC CTCAGGTTTT GGCTGTTCGG CGGCCACACC TGTGTTTGGC 
CAACAATCCA CCACTGGGAG CATATTTGGA CAGCAACAGC AACCTTCAAC TGGTGGAGGT 
TTGTTCGGTC AAAGTTCGAC CGGCGCTGCA ACCTCGTCCT CTGGCGGATT CTTCAGCGGG 
TTGGGAGGCA AACCCAGTGA AGACGCTGCC AACAAGAACC CATTTGGTAC CACCGCCTCC 
ACTGGCGTGT TTGGCCAGAG CACGCAGTCT GGGCCCAACA GCCTCTTTGG GAGCAGTAGC 
GCCAAGACCT TTGGATTTGG TCAGCCCTCC TTCGGGGAGC AGAAGCCCAG TGGGACCTTC 
AGTACTGGTG GAGGCAGTGT TGCATGTCAG GGCTTTGGTT CCTTCTCCAC ACCATCTAAA 
TCAACGAGCG GCTTTGGCAG CGCCCCCGTG TTCGGAAGCC CCCCTTCTTT TGGCGGTTCT 
CCTGCATTCG GTGGCGGCGC TTCATTCAGT TCAAGTCCGT CTTTCAGCAA CCCTTTAGCT 
TCCCAGTCCA GTAAAGTGTT TGGCGAGGGA ACAGCAGCCG CCAGCATGGG AGGATTTGGT 
TTCTCCTCAC CCCCCAGTGC CCCCTCCTTT GGGACTCTCG CCACTCAGAC TGCGCCGTCA 
TTCGGGAGCT TGGCCCAGCA GGGCCCTGGA TTTGGAAGCC AGCCCAGCAG CTTCTCAGGC 
TTTGGCCAGC AGCCACAGAC GGGAGGATTT TCAGGAAACG CATTTGGATC TCCAAATCCA 
CCGGGTGCTC CGTCGTTTGG CAGCTGGAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481723.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480025.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.

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CloneID	OJa107476
Clone ID Related Accession (Same CDS sequence)	XM_011481723.3
Accession Version	XM_011481723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011481723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCATTAC AACAAAGTTG GATTAACAAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480025.1
CDS	122..6097
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPLQQSWINNL

Target ORF information:

RefSeq Version	XM_011481723.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481723.3

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCATTAC AACAAAGTTG GATTAACAAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481725.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480027.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.

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CloneID	OJa107477
Clone ID Related Accession (Same CDS sequence)	XM_011481725.3
Accession Version	XM_011481725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011481725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TTCCGTGGCA ACAGCACCAG CCAAGATTCC TGCTGCAGTT 
CCTTCTGCTC CACCTGCCTT CCCGAGCATC AGCCTCGCTT CTTCAGCAGC TGCGCCCAGT 
CCTCCAGTCT CTTCTGCTCC GTCCTCCATT ATTCCTTTTA GCATGGCTCC AACGGCTCCT 
GTGACATCAG CCTTCTCCGG CTTTAGTTTC TCAGTCCCAT CAACCTTCTC CACCACCTCT 
GCCCCAGTTG CAGCTTTCGC CTTGGGAGGA TCTGTGCCTT TTGGCTCAGG GTCCTCTGGC 
TTCTCATTTG CCTCCAAACC TCCGTCTGAG ACCCCGTCCG CACTCTCGGC GTTTTCGTTT 
AATTCCCCTT CCGGCAAACC ACCAGCAGTG GCTGCCCCGT CTGCTGCTGT GGCACCTCAG 
AGCCTTTCAA CAGCCTCACC GCCCACAGTC AAACTTAGTC TGAATGAAAG ATTTTCAGGG 
GTGGAGGCTT CAGCTCCGGC TTTCTCTTTG CCTAAAGCTG TTGTTTCCAG CTCCAGTAGT 
TTCAATGTGC CTCCAATCTC AGCCTCAAAG CCGCCAGCTG TATGCGCTCC AGTGCACCCG 
GTGCAGACTA GTACACCTCC AGCCAGTGTC CAGAAACCTG CTCCTGCTGT CCAAGGACGT 
GTCTCTACTA TGCAGCAAGC CGTTAGCGTG AAAGCCCTGG AGAAACAGCT GCAGCAAAAG 
AAAGACTCTG ATCCCATTAT GGCTGGAATT TTGGAAGAGA TTGCACATTT TCAGAAAGAG 
TTAGATGACC TGAAGGCACG CAGTTCAAAA GCTGACGTCA GGGTCGGCAC AAACGATGAC 
ATGAAGGATC TGAGGAAAGA GTCTGAGGAC CTCCACATCT TCACTCTGGA GATTAAAGAA 
ACGACAGAGT CTCTCCATGG TGACATTGGC ACGCTGAAGA CCACATTACT GGAGGGCTTT 
GCTGGGGCGG AAGAAGCCAA GACGCAGAAC GAGCTGGGAA AAGATAAAAA CTACAGACAG 
CTGTTGTACA AAAAACCTCT GGATCCCAGA AGTGAGGAGC AACTCAAGGA GATTCGGAGG 
CTGTACCAGT ACGTTAAATT TGCTGTGGAG GATGTTAACG ACGTGTTGGA TGTGGAATGG 
GAGAAACACC TGGAGAAAAA GAAAAAGCAA AAACACATGG TCGTGCCTGG ACGTGAGGGT 
CTTTTCAATA CATTGTCCAA CAACCTGTGC ATCATCAACC AGCAGAAGAA CCGACTGGAT 
CAGCTGATTC AGGAGCTCAG TGCTCTGCGG CTCTACAGCA AAACCACTGC TCCTACCATT 
AACCAGAACA CTGCAGCAAC ATCAGATGAC TACAAAGGTG AGCTGGAGAA TTTGGAGGAG 
GCACTTCTGA AAGCCCGGCT TGAAACGACT CCTCCTGAGA CAAAAGTGAA ATCAGCAGCA 
GTCAAGGTTT CTCCTCTTAA ACAGTCCCAA CTGCGCAACT TCCTGTCAAA AGGGAAAATG 
CCTCCTGTGC GTTCAACTGC ACCAGCCAAC TTGTCGCGCT CGGCCTTCCT GGCCCCTAAA 
AACCTTGAGG ACTTGGATGA TGTGAGCTCA ACGTCCTCCT TATCGCAGTC TATAGAGCTT 
CTCCCATCTC ACTTGGAGTT GGAGGAAGAG GAAGAACTAC AGCCCCTGCA GCCTTCACCT 
ATGAATTCCA TGACCCAATC ACTTCCCACC CCACGTCACC CCACTGTTGT TCGAACGCCC 
TCCATTCAGC CAGGCTTTGG AGCTATACAG TCCACCCCTC TAACAAAGAT GCACCCTGTT 
CCTGGTATGG GCTTTGTGCT AAGCCCCATT ATCAACCCTG TTCCTACAAA TAGGATTAAC 
CTCAGCGGGG CTGAAAGTAC TGCGCTTGCC ACAAAGACGG TAAAGCATGG AGCACCACCT 
GCAGAGCGGA GCATCCCTGT GACCATCTCT GCCCAGCAGG CTGCTGCCAC CGCGGCTCTG 
CGGAGACAAA TGGCCAATCA GAAGACGGCT GTTGTCAACA CTTCTTTGAC AGAGTCCACC 
TTAAAAACTG TCCCTCAAGT AGTGAATGTT CAGGAGCTTA AGGACAAAGG GGCACCTATG 
CCAGTGTCCT CTATGGTCAG CTCCTCGGCG CCAGATCCCT CAGTCCAGGG CTTCACAGCA 
GTTTCTAGCA GCCAAGTCAA ACGAAACACA CCGGGTCTGC AAAAGATACC CACAGAGAAT 
GCATCTATTC CACAGACAGG CTTTGTCTTT GGCCAACCAT CCAAAACAGA CCTTTCCGGG 
GGTCCACCTA GTTCACTTGA GCAAAACACC AGCAAGGCCT TCTCATTCAC CTCAGGATCG 
ACGGGCTTCA GTTTTTCATC TGTAACACAA GGTGCTGGAT TGTCTCAAGG GAAGGATGTG 
AACAAGTTCT CCATTGGCGG AAATGGCAAA GGGTTGTTTG GCCAAACTGG AGAGGAGCCA 
TTCTCCCTTC CCACTAAGTC AAACTCTCCT GGTCTGAGCA ATGGGTCTTC AAGCTCATCG 
GCCAACAGTT TAGGGGAAGT AGCAAAACAG GGTTCCACTG TTACTGGTTC TAAGACAGAG 
CCGCAGCCTC TAAAGGCAGC TGGAGGGGAG ACATTAGGCA GTTTTTCAGG ACTACGAGTG 
GGACACGGAG AAGAAGCAAA CGAATCCTCC ACAAAGACCA CTGCTCCTGC ATTTTCCTTC 
GGGAATGCTG GACTTGGCCA TAGCAAGTCA GCTCCTCAGT TTAACTTTGG TTCAACTCCC 
CTTAAGTCTG CCGAAGACGG GGCTGCAGCA GACATGCCTA AGGTGTCAGG ATCAGGCAGC 
TTGTTTAAAC CTCCTGATTC AAACTCCATG CCAACCTTTT CTATAACCCC TTCAACTACA 
GCTGCTTCAG CGCTGCCTAC ATCTTTAAGT AGTCTTCTTG CAGCTCCTAC TGAACAGCCT 
AAAGCTACTC AGCAACCTTC AGAAAGCACA TCGGCTGCTT TACCAGAACT GAGTACCAAG 
GAACTTGCTG TAGACCTGGC TAGTGTTCCG GCAGAACCTG AGCCTCTAGT GACTGCAGTT 
GCCACAACAA CCACGCCAAC ATCTGCAGCA CCAACACTTC CTATGGTCAC TCCTGTCCCT 
ACTGCAGACT CCTCTTCTAC AGTTCCTGCA GAGACGGAAA CAACTCTGCC TCCAGCACCA 
CCAACTGCTT CTTCCACAGA AGAAGCCACC CCAGACCCCT CTTCTACACC TGCCGTCTCC 
ACATCTACCA CCGCTGTCCC ACTTACCATC GAGGTAACCT CAGCAGCTTT ACCAGCTTCC 
TCCTCAGAAA AACCAGGCTC CATTTTTACC TCTGCTACTC CTGCAACCAC CACTAGCAAT 
TCTGTTGGGG CCATACCAGC CATTAACACA GCTGCTCCCA CAACCACTAC CGCTGCAGTT 
ATCACCAGTG CAGCAACGAC AGCTGCTAAT GTGTTTGGGC AGCCTGCCGC TCCTCCTGCC 
TCTTCTGCCC CTGCAACTAC CGGATTCGGC TCATCTGGAT TTGGCGCACC AACAGAAACC 
GGTTTCGGCA AATCTGTATT TGGCGTGGGT GGGTTTGGAC AGCCTGCTAG CATAGGAGGA 
ACTTCGAGTG GCTTTTCATT CACCCAGTCA GCATTCGGAG CAAGTTCAAG CAGTGCGACC 
ACTGGAGGAG GTGGTCTTTT CGGTGCTCCC ACTGTTACCA ATGCAAGCTC CTTCTCCTTC 
GGCACCAGCA GTTCCAGCAC ACCCAGCAGC ACCGGGCTGT TTAGCCAAAG CACCCCGTCA 
GTATTCGGCC AGAGCTCTGG ATTTGGGCAA GGATCTATGT TCAGAAATAA CACCACCACA 
TCATCTGCGT CAGGCTTCAG CTTTGGACAG CCCTCAGGTT TTGGCTGTTC GGCGGCCACA 
CCTGTGTTTG GCCAACAATC CACCACTGGG AGCATATTTG GACAGCAACA GCAACCTTCA 
ACTGGTGGAG GTTTGTTCGG TCAAAGTTCG ACCGGCGCTG CAACCTCGTC CTCTGGCGGA 
TTCTTCAGCG GGTTGGGAGG CAAACCCAGT GAAGACGCTG CCAACAAGAA CCCATTTGGT 
ACCACCGCCT CCACTGGCGT GTTTGGCCAG AGCACGCAGT CTGGGCCCAA CAGCCTCTTT 
GGGAGCAGTA GCGCCAAGAC CTTTGGATTT GGTCAGCCCT CCTTCGGGGA GCAGAAGCCC 
AGTGGGACCT TCAGTACTGG TGGAGGCAGT GTTGCATGTC AGGGCTTTGG TTCCTTCTCC 
ACACCATCTA AATCAACGAG CGGCTTTGGC AGCGCCCCCG TGTTCGGAAG CCCCCCTTCT 
TTTGGCGGTT CTCCTGCATT CGGTGGCGGC GCTTCATTCA GTTCAAGTCC GTCTTTCAGC 
AACCCTTTAG CTTCCCAGTC CAGTAAAGTG TTTGGCGAGG GAACAGCAGC CGCCAGCATG 
GGAGGATTTG GTTTCTCCTC ACCCCCCAGT GCCCCCTCCT TTGGGACTCT CGCCACTCAG 
ACTGCGCCGT CATTCGGGAG CTTGGCCCAG CAGGGCCCTG GATTTGGAAG CCAGCCCAGC 
AGCTTCTCAG GCTTTGGCCA GCAGCCACAG ACGGGAGGAT TTTCAGGAAA CGCATTTGGA 
TCTCCAAATC CACCGGGTGC TCCGTCGTTT GGCAGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480027.1
CDS	121..6048
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481725.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481725.3

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TCTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TTCCGTGGCA ACAGCACCAG CCAAGATTCC TGCTGCAGTT 
CCTTCTGCTC CACCTGCCTT CCCGAGCATC AGCCTCGCTT CTTCAGCAGC TGCGCCCAGT 
CCTCCAGTCT CTTCTGCTCC GTCCTCCATT ATTCCTTTTA GCATGGCTCC AACGGCTCCT 
GTGACATCAG CCTTCTCCGG CTTTAGTTTC TCAGTCCCAT CAACCTTCTC CACCACCTCT 
GCCCCAGTTG CAGCTTTCGC CTTGGGAGGA TCTGTGCCTT TTGGCTCAGG GTCCTCTGGC 
TTCTCATTTG CCTCCAAACC TCCGTCTGAG ACCCCGTCCG CACTCTCGGC GTTTTCGTTT 
AATTCCCCTT CCGGCAAACC ACCAGCAGTG GCTGCCCCGT CTGCTGCTGT GGCACCTCAG 
AGCCTTTCAA CAGCCTCACC GCCCACAGTC AAACTTAGTC TGAATGAAAG ATTTTCAGGG 
GTGGAGGCTT CAGCTCCGGC TTTCTCTTTG CCTAAAGCTG TTGTTTCCAG CTCCAGTAGT 
TTCAATGTGC CTCCAATCTC AGCCTCAAAG CCGCCAGCTG TATGCGCTCC AGTGCACCCG 
GTGCAGACTA GTACACCTCC AGCCAGTGTC CAGAAACCTG CTCCTGCTGT CCAAGGACGT 
GTCTCTACTA TGCAGCAAGC CGTTAGCGTG AAAGCCCTGG AGAAACAGCT GCAGCAAAAG 
AAAGACTCTG ATCCCATTAT GGCTGGAATT TTGGAAGAGA TTGCACATTT TCAGAAAGAG 
TTAGATGACC TGAAGGCACG CAGTTCAAAA GCTGACGTCA GGGTCGGCAC AAACGATGAC 
ATGAAGGATC TGAGGAAAGA GTCTGAGGAC CTCCACATCT TCACTCTGGA GATTAAAGAA 
ACGACAGAGT CTCTCCATGG TGACATTGGC ACGCTGAAGA CCACATTACT GGAGGGCTTT 
GCTGGGGCGG AAGAAGCCAA GACGCAGAAC GAGCTGGGAA AAGATAAAAA CTACAGACAG 
CTGTTGTACA AAAAACCTCT GGATCCCAGA AGTGAGGAGC AACTCAAGGA GATTCGGAGG 
CTGTACCAGT ACGTTAAATT TGCTGTGGAG GATGTTAACG ACGTGTTGGA TGTGGAATGG 
GAGAAACACC TGGAGAAAAA GAAAAAGCAA AAACACATGG TCGTGCCTGG ACGTGAGGGT 
CTTTTCAATA CATTGTCCAA CAACCTGTGC ATCATCAACC AGCAGAAGAA CCGACTGGAT 
CAGCTGATTC AGGAGCTCAG TGCTCTGCGG CTCTACAGCA AAACCACTGC TCCTACCATT 
AACCAGAACA CTGCAGCAAC ATCAGATGAC TACAAAGGTG AGCTGGAGAA TTTGGAGGAG 
GCACTTCTGA AAGCCCGGCT TGAAACGACT CCTCCTGAGA CAAAAGTGAA ATCAGCAGCA 
GTCAAGGTTT CTCCTCTTAA ACAGTCCCAA CTGCGCAACT TCCTGTCAAA AGGGAAAATG 
CCTCCTGTGC GTTCAACTGC ACCAGCCAAC TTGTCGCGCT CGGCCTTCCT GGCCCCTAAA 
AACCTTGAGG ACTTGGATGA TGTGAGCTCA ACGTCCTCCT TATCGCAGTC TATAGAGCTT 
CTCCCATCTC ACTTGGAGTT GGAGGAAGAG GAAGAACTAC AGCCCCTGCA GCCTTCACCT 
ATGAATTCCA TGACCCAATC ACTTCCCACC CCACGTCACC CCACTGTTGT TCGAACGCCC 
TCCATTCAGC CAGGCTTTGG AGCTATACAG TCCACCCCTC TAACAAAGAT GCACCCTGTT 
CCTGGTATGG GCTTTGTGCT AAGCCCCATT ATCAACCCTG TTCCTACAAA TAGGATTAAC 
CTCAGCGGGG CTGAAAGTAC TGCGCTTGCC ACAAAGACGG TAAAGCATGG AGCACCACCT 
GCAGAGCGGA GCATCCCTGT GACCATCTCT GCCCAGCAGG CTGCTGCCAC CGCGGCTCTG 
CGGAGACAAA TGGCCAATCA GAAGACGGCT GTTGTCAACA CTTCTTTGAC AGAGTCCACC 
TTAAAAACTG TCCCTCAAGT AGTGAATGTT CAGGAGCTTA AGGACAAAGG GGCACCTATG 
CCAGTGTCCT CTATGGTCAG CTCCTCGGCG CCAGATCCCT CAGTCCAGGG CTTCACAGCA 
GTTTCTAGCA GCCAAGTCAA ACGAAACACA CCGGGTCTGC AAAAGATACC CACAGAGAAT 
GCATCTATTC CACAGACAGG CTTTGTCTTT GGCCAACCAT CCAAAACAGA CCTTTCCGGG 
GGTCCACCTA GTTCACTTGA GCAAAACACC AGCAAGGCCT TCTCATTCAC CTCAGGATCG 
ACGGGCTTCA GTTTTTCATC TGTAACACAA GGTGCTGGAT TGTCTCAAGG GAAGGATGTG 
AACAAGTTCT CCATTGGCGG AAATGGCAAA GGGTTGTTTG GCCAAACTGG AGAGGAGCCA 
TTCTCCCTTC CCACTAAGTC AAACTCTCCT GGTCTGAGCA ATGGGTCTTC AAGCTCATCG 
GCCAACAGTT TAGGGGAAGT AGCAAAACAG GGTTCCACTG TTACTGGTTC TAAGACAGAG 
CCGCAGCCTC TAAAGGCAGC TGGAGGGGAG ACATTAGGCA GTTTTTCAGG ACTACGAGTG 
GGACACGGAG AAGAAGCAAA CGAATCCTCC ACAAAGACCA CTGCTCCTGC ATTTTCCTTC 
GGGAATGCTG GACTTGGCCA TAGCAAGTCA GCTCCTCAGT TTAACTTTGG TTCAACTCCC 
CTTAAGTCTG CCGAAGACGG GGCTGCAGCA GACATGCCTA AGGTGTCAGG ATCAGGCAGC 
TTGTTTAAAC CTCCTGATTC AAACTCCATG CCAACCTTTT CTATAACCCC TTCAACTACA 
GCTGCTTCAG CGCTGCCTAC ATCTTTAAGT AGTCTTCTTG CAGCTCCTAC TGAACAGCCT 
AAAGCTACTC AGCAACCTTC AGAAAGCACA TCGGCTGCTT TACCAGAACT GAGTACCAAG 
GAACTTGCTG TAGACCTGGC TAGTGTTCCG GCAGAACCTG AGCCTCTAGT GACTGCAGTT 
GCCACAACAA CCACGCCAAC ATCTGCAGCA CCAACACTTC CTATGGTCAC TCCTGTCCCT 
ACTGCAGACT CCTCTTCTAC AGTTCCTGCA GAGACGGAAA CAACTCTGCC TCCAGCACCA 
CCAACTGCTT CTTCCACAGA AGAAGCCACC CCAGACCCCT CTTCTACACC TGCCGTCTCC 
ACATCTACCA CCGCTGTCCC ACTTACCATC GAGGTAACCT CAGCAGCTTT ACCAGCTTCC 
TCCTCAGAAA AACCAGGCTC CATTTTTACC TCTGCTACTC CTGCAACCAC CACTAGCAAT 
TCTGTTGGGG CCATACCAGC CATTAACACA GCTGCTCCCA CAACCACTAC CGCTGCAGTT 
ATCACCAGTG CAGCAACGAC AGCTGCTAAT GTGTTTGGGC AGCCTGCCGC TCCTCCTGCC 
TCTTCTGCCC CTGCAACTAC CGGATTCGGC TCATCTGGAT TTGGCGCACC AACAGAAACC 
GGTTTCGGCA AATCTGTATT TGGCGTGGGT GGGTTTGGAC AGCCTGCTAG CATAGGAGGA 
ACTTCGAGTG GCTTTTCATT CACCCAGTCA GCATTCGGAG CAAGTTCAAG CAGTGCGACC 
ACTGGAGGAG GTGGTCTTTT CGGTGCTCCC ACTGTTACCA ATGCAAGCTC CTTCTCCTTC 
GGCACCAGCA GTTCCAGCAC ACCCAGCAGC ACCGGGCTGT TTAGCCAAAG CACCCCGTCA 
GTATTCGGCC AGAGCTCTGG ATTTGGGCAA GGATCTATGT TCAGAAATAA CACCACCACA 
TCATCTGCGT CAGGCTTCAG CTTTGGACAG CCCTCAGGTT TTGGCTGTTC GGCGGCCACA 
CCTGTGTTTG GCCAACAATC CACCACTGGG AGCATATTTG GACAGCAACA GCAACCTTCA 
ACTGGTGGAG GTTTGTTCGG TCAAAGTTCG ACCGGCGCTG CAACCTCGTC CTCTGGCGGA 
TTCTTCAGCG GGTTGGGAGG CAAACCCAGT GAAGACGCTG CCAACAAGAA CCCATTTGGT 
ACCACCGCCT CCACTGGCGT GTTTGGCCAG AGCACGCAGT CTGGGCCCAA CAGCCTCTTT 
GGGAGCAGTA GCGCCAAGAC CTTTGGATTT GGTCAGCCCT CCTTCGGGGA GCAGAAGCCC 
AGTGGGACCT TCAGTACTGG TGGAGGCAGT GTTGCATGTC AGGGCTTTGG TTCCTTCTCC 
ACACCATCTA AATCAACGAG CGGCTTTGGC AGCGCCCCCG TGTTCGGAAG CCCCCCTTCT 
TTTGGCGGTT CTCCTGCATT CGGTGGCGGC GCTTCATTCA GTTCAAGTCC GTCTTTCAGC 
AACCCTTTAG CTTCCCAGTC CAGTAAAGTG TTTGGCGAGG GAACAGCAGC CGCCAGCATG 
GGAGGATTTG GTTTCTCCTC ACCCCCCAGT GCCCCCTCCT TTGGGACTCT CGCCACTCAG 
ACTGCGCCGT CATTCGGGAG CTTGGCCCAG CAGGGCCCTG GATTTGGAAG CCAGCCCAGC 
AGCTTCTCAG GCTTTGGCCA GCAGCCACAG ACGGGAGGAT TTTCAGGAAA CGCATTTGGA 
TCTCCAAATC CACCGGGTGC TCCGTCGTTT GGCAGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481722.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480024.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.

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CloneID	OJa33580
Clone ID Related Accession (Same CDS sequence)	XM_011481722.2
Accession Version	XM_011481722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011481722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCGGGTG CTCCGTCGTT TGGCAGCTGG AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480024.1
CDS	148..6126
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481722.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481722.2

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCGGGTG CTCCGTCGTT TGGCAGCTGG AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481723.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480025.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.

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CloneID	OJa33581
Clone ID Related Accession (Same CDS sequence)	XM_011481723.2
Accession Version	XM_011481723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011481723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCATTAC AACAAAGTTG GATTAACAAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480025.1
CDS	148..6123
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPLQQSWINNL

Target ORF information:

RefSeq Version	XM_011481723.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481723.2

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGC AGTCAAGGTT 
TCTCCTCTTA AACAGTCCCA ACTGCGCAAC TTCCTGTCAA AAGGGAAAAT GCCTCCTGTG 
CGTTCAACTG CACCAGCCAA CTTGTCGCGC TCGGCCTTCC TGGCCCCTAA AAACCTTGAG 
GACTTGGATG ATGTGAGCTC AACGTCCTCC TTATCGCAGT CTATAGAGCT TCTCCCATCT 
CACTTGGAGT TGGAGGAAGA GGAAGAACTA CAGCCCCTGC AGCCTTCACC TATGAATTCC 
ATGACCCAAT CACTTCCCAC CCCACGTCAC CCCACTGTTG TTCGAACGCC CTCCATTCAG 
CCAGGCTTTG GAGCTATACA GTCCACCCCT CTAACAAAGA TGCACCCTGT TCCTGGTATG 
GGCTTTGTGC TAAGCCCCAT TATCAACCCT GTTCCTACAA ATAGGATTAA CCTCAGCGGG 
GCTGAAAGTA CTGCGCTTGC CACAAAGACG GTAAAGCATG GAGCACCACC TGCAGAGCGG 
AGCATCCCTG TGACCATCTC TGCCCAGCAG GCTGCTGCCA CCGCGGCTCT GCGGAGACAA 
ATGGCCAATC AGAAGACGGC TGTTGTCAAC ACTTCTTTGA CAGAGTCCAC CTTAAAAACT 
GTCCCTCAAG TAGTGAATGT TCAGGAGCTT AAGGACAAAG GGGCACCTAT GCCAGTGTCC 
TCTATGGTCA GCTCCTCGGC GCCAGATCCC TCAGTCCAGG GCTTCACAGC AGTTTCTAGC 
AGCCAAGTCA AACGAAACAC ACCGGGTCTG CAAAAGATAC CCACAGAGAA TGCATCTATT 
CCACAGACAG GCTTTGTCTT TGGCCAACCA TCCAAAACAG ACCTTTCCGG GGGTCCACCT 
AGTTCACTTG AGCAAAACAC CAGCAAGGCC TTCTCATTCA CCTCAGGATC GACGGGCTTC 
AGTTTTTCAT CTGTAACACA AGGTGCTGGA TTGTCTCAAG GGAAGGATGT GAACAAGTTC 
TCCATTGGCG GAAATGGCAA AGGGTTGTTT GGCCAAACTG GAGAGGAGCC ATTCTCCCTT 
CCCACTAAGT CAAACTCTCC TGGTCTGAGC AATGGGTCTT CAAGCTCATC GGCCAACAGT 
TTAGGGGAAG TAGCAAAACA GGGTTCCACT GTTACTGGTT CTAAGACAGA GCCGCAGCCT 
CTAAAGGCAG CTGGAGGGGA GACATTAGGC AGTTTTTCAG GACTACGAGT GGGACACGGA 
GAAGAAGCAA ACGAATCCTC CACAAAGACC ACTGCTCCTG CATTTTCCTT CGGGAATGCT 
GGACTTGGCC ATAGCAAGTC AGCTCCTCAG TTTAACTTTG GTTCAACTCC CCTTAAGTCT 
GCCGAAGACG GGGCTGCAGC AGACATGCCT AAGGTGTCAG GATCAGGCAG CTTGTTTAAA 
CCTCCTGATT CAAACTCCAT GCCAACCTTT TCTATAACCC CTTCAACTAC AGCTGCTTCA 
GCGCTGCCTA CATCTTTAAG TAGTCTTCTT GCAGCTCCTA CTGAACAGCC TAAAGCTACT 
CAGCAACCTT CAGAAAGCAC ATCGGCTGCT TTACCAGAAC TGAGTACCAA GGAACTTGCT 
GTAGACCTGG CTAGTGTTCC GGCAGAACCT GAGCCTCTAG TGACTGCAGT TGCCACAACA 
ACCACGCCAA CATCTGCAGC ACCAACACTT CCTATGGTCA CTCCTGTCCC TACTGCAGAC 
TCCTCTTCTA CAGTTCCTGC AGAGACGGAA ACAACTCTGC CTCCAGCACC ACCAACTGCT 
TCTTCCACAG AAGAAGCCAC CCCAGACCCC TCTTCTACAC CTGCCGTCTC CACATCTACC 
ACCGCTGTCC CACTTACCAT CGAGGTAACC TCAGCAGCTT TACCAGCTTC CTCCTCAGAA 
AAACCAGGCT CCATTTTTAC CTCTGCTACT CCTGCAACCA CCACTAGCAA TTCTGTTGGG 
GCCATACCAG CCATTAACAC AGCTGCTCCC ACAACCACTA CCGCTGCAGT TATCACCAGT 
GCAGCAACGA CAGCTGCTAA TGTGTTTGGG CAGCCTGCCG CTCCTCCTGC CTCTTCTGCC 
CCTGCAACTA CCGGATTCGG CTCATCTGGA TTTGGCGCAC CAACAGAAAC CGGTTTCGGC 
AAATCTGTAT TTGGCGTGGG TGGGTTTGGA CAGCCTGCTA GCATAGGAGG AACTTCGAGT 
GGCTTTTCAT TCACCCAGTC AGCATTCGGA GCAAGTTCAA GCAGTGCGAC CACTGGAGGA 
GGTGGTCTTT TCGGTGCTCC CACTGTTACC AATGCAAGCT CCTTCTCCTT CGGCACCAGC 
AGTTCCAGCA CACCCAGCAG CACCGGGCTG TTTAGCCAAA GCACCCCGTC AGTATTCGGC 
CAGAGCTCTG GATTTGGGCA AGGATCTATG TTCAGAAATA ACACCACCAC ATCATCTGCG 
TCAGGCTTCA GCTTTGGACA GCCCTCAGGT TTTGGCTGTT CGGCGGCCAC ACCTGTGTTT 
GGCCAACAAT CCACCACTGG GAGCATATTT GGACAGCAAC AGCAACCTTC AACTGGTGGA 
GGTTTGTTCG GTCAAAGTTC GACCGGCGCT GCAACCTCGT CCTCTGGCGG ATTCTTCAGC 
GGGTTGGGAG GCAAACCCAG TGAAGACGCT GCCAACAAGA ACCCATTTGG TACCACCGCC 
TCCACTGGCG TGTTTGGCCA GAGCACGCAG TCTGGGCCCA ACAGCCTCTT TGGGAGCAGT 
AGCGCCAAGA CCTTTGGATT TGGTCAGCCC TCCTTCGGGG AGCAGAAGCC CAGTGGGACC 
TTCAGTACTG GTGGAGGCAG TGTTGCATGT CAGGGCTTTG GTTCCTTCTC CACACCATCT 
AAATCAACGA GCGGCTTTGG CAGCGCCCCC GTGTTCGGAA GCCCCCCTTC TTTTGGCGGT 
TCTCCTGCAT TCGGTGGCGG CGCTTCATTC AGTTCAAGTC CGTCTTTCAG CAACCCTTTA 
GCTTCCCAGT CCAGTAAAGT GTTTGGCGAG GGAACAGCAG CCGCCAGCAT GGGAGGATTT 
GGTTTCTCCT CACCCCCCAG TGCCCCCTCC TTTGGGACTC TCGCCACTCA GACTGCGCCG 
TCATTCGGGA GCTTGGCCCA GCAGGGCCCT GGATTTGGAA GCCAGCCCAG CAGCTTCTCA 
GGCTTTGGCC AGCAGCCACA GACGGGAGGA TTTTCAGGAA ACGCATTTGG ATCTCCAAAT 
CCACCATTAC AACAAAGTTG GATTAACAAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481724.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480026.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.

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CloneID	OJa33582
Clone ID Related Accession (Same CDS sequence)	XM_011481724.2
Accession Version	XM_011481724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011481724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGT CAAGGTTTCT 
CCTCTTAAAC AGTCCCAACT GCGCAACTTC CTGTCAAAAG GGAAAATGCC TCCTGTGCGT 
TCAACTGCAC CAGCCAACTT GTCGCGCTCG GCCTTCCTGG CCCCTAAAAA CCTTGAGGAC 
TTGGATGATG TGAGCTCAAC GTCCTCCTTA TCGCAGTCTA TAGAGCTTCT CCCATCTCAC 
TTGGAGTTGG AGGAAGAGGA AGAACTACAG CCCCTGCAGC CTTCACCTAT GAATTCCATG 
ACCCAATCAC TTCCCACCCC ACGTCACCCC ACTGTTGTTC GAACGCCCTC CATTCAGCCA 
GGCTTTGGAG CTATACAGTC CACCCCTCTA ACAAAGATGC ACCCTGTTCC TGGTATGGGC 
TTTGTGCTAA GCCCCATTAT CAACCCTGTT CCTACAAATA GGATTAACCT CAGCGGGGCT 
GAAAGTACTG CGCTTGCCAC AAAGACGGTA AAGCATGGAG CACCACCTGC AGAGCGGAGC 
ATCCCTGTGA CCATCTCTGC CCAGCAGGCT GCTGCCACCG CGGCTCTGCG GAGACAAATG 
GCCAATCAGA AGACGGCTGT TGTCAACACT TCTTTGACAG AGTCCACCTT AAAAACTGTC 
CCTCAAGTAG TGAATGTTCA GGAGCTTAAG GACAAAGGGG CACCTATGCC AGTGTCCTCT 
ATGGTCAGCT CCTCGGCGCC AGATCCCTCA GTCCAGGGCT TCACAGCAGT TTCTAGCAGC 
CAAGTCAAAC GAAACACACC GGGTCTGCAA AAGATACCCA CAGAGAATGC ATCTATTCCA 
CAGACAGGCT TTGTCTTTGG CCAACCATCC AAAACAGACC TTTCCGGGGG TCCACCTAGT 
TCACTTGAGC AAAACACCAG CAAGGCCTTC TCATTCACCT CAGGATCGAC GGGCTTCAGT 
TTTTCATCTG TAACACAAGG TGCTGGATTG TCTCAAGGGA AGGATGTGAA CAAGTTCTCC 
ATTGGCGGAA ATGGCAAAGG GTTGTTTGGC CAAACTGGAG AGGAGCCATT CTCCCTTCCC 
ACTAAGTCAA ACTCTCCTGG TCTGAGCAAT GGGTCTTCAA GCTCATCGGC CAACAGTTTA 
GGGGAAGTAG CAAAACAGGG TTCCACTGTT ACTGGTTCTA AGACAGAGCC GCAGCCTCTA 
AAGGCAGCTG GAGGGGAGAC ATTAGGCAGT TTTTCAGGAC TACGAGTGGG ACACGGAGAA 
GAAGCAAACG AATCCTCCAC AAAGACCACT GCTCCTGCAT TTTCCTTCGG GAATGCTGGA 
CTTGGCCATA GCAAGTCAGC TCCTCAGTTT AACTTTGGTT CAACTCCCCT TAAGTCTGCC 
GAAGACGGGG CTGCAGCAGA CATGCCTAAG GTGTCAGGAT CAGGCAGCTT GTTTAAACCT 
CCTGATTCAA ACTCCATGCC AACCTTTTCT ATAACCCCTT CAACTACAGC TGCTTCAGCG 
CTGCCTACAT CTTTAAGTAG TCTTCTTGCA GCTCCTACTG AACAGCCTAA AGCTACTCAG 
CAACCTTCAG AAAGCACATC GGCTGCTTTA CCAGAACTGA GTACCAAGGA ACTTGCTGTA 
GACCTGGCTA GTGTTCCGGC AGAACCTGAG CCTCTAGTGA CTGCAGTTGC CACAACAACC 
ACGCCAACAT CTGCAGCACC AACACTTCCT ATGGTCACTC CTGTCCCTAC TGCAGACTCC 
TCTTCTACAG TTCCTGCAGA GACGGAAACA ACTCTGCCTC CAGCACCACC AACTGCTTCT 
TCCACAGAAG AAGCCACCCC AGACCCCTCT TCTACACCTG CCGTCTCCAC ATCTACCACC 
GCTGTCCCAC TTACCATCGA GGTAACCTCA GCAGCTTTAC CAGCTTCCTC CTCAGAAAAA 
CCAGGCTCCA TTTTTACCTC TGCTACTCCT GCAACCACCA CTAGCAATTC TGTTGGGGCC 
ATACCAGCCA TTAACACAGC TGCTCCCACA ACCACTACCG CTGCAGTTAT CACCAGTGCA 
GCAACGACAG CTGCTAATGT GTTTGGGCAG CCTGCCGCTC CTCCTGCCTC TTCTGCCCCT 
GCAACTACCG GATTCGGCTC ATCTGGATTT GGCGCACCAA CAGAAACCGG TTTCGGCAAA 
TCTGTATTTG GCGTGGGTGG GTTTGGACAG CCTGCTAGCA TAGGAGGAAC TTCGAGTGGC 
TTTTCATTCA CCCAGTCAGC ATTCGGAGCA AGTTCAAGCA GTGCGACCAC TGGAGGAGGT 
GGTCTTTTCG GTGCTCCCAC TGTTACCAAT GCAAGCTCCT TCTCCTTCGG CACCAGCAGT 
TCCAGCACAC CCAGCAGCAC CGGGCTGTTT AGCCAAAGCA CCCCGTCAGT ATTCGGCCAG 
AGCTCTGGAT TTGGGCAAGG ATCTATGTTC AGAAATAACA CCACCACATC ATCTGCGTCA 
GGCTTCAGCT TTGGACAGCC CTCAGGTTTT GGCTGTTCGG CGGCCACACC TGTGTTTGGC 
CAACAATCCA CCACTGGGAG CATATTTGGA CAGCAACAGC AACCTTCAAC TGGTGGAGGT 
TTGTTCGGTC AAAGTTCGAC CGGCGCTGCA ACCTCGTCCT CTGGCGGATT CTTCAGCGGG 
TTGGGAGGCA AACCCAGTGA AGACGCTGCC AACAAGAACC CATTTGGTAC CACCGCCTCC 
ACTGGCGTGT TTGGCCAGAG CACGCAGTCT GGGCCCAACA GCCTCTTTGG GAGCAGTAGC 
GCCAAGACCT TTGGATTTGG TCAGCCCTCC TTCGGGGAGC AGAAGCCCAG TGGGACCTTC 
AGTACTGGTG GAGGCAGTGT TGCATGTCAG GGCTTTGGTT CCTTCTCCAC ACCATCTAAA 
TCAACGAGCG GCTTTGGCAG CGCCCCCGTG TTCGGAAGCC CCCCTTCTTT TGGCGGTTCT 
CCTGCATTCG GTGGCGGCGC TTCATTCAGT TCAAGTCCGT CTTTCAGCAA CCCTTTAGCT 
TCCCAGTCCA GTAAAGTGTT TGGCGAGGGA ACAGCAGCCG CCAGCATGGG AGGATTTGGT 
TTCTCCTCAC CCCCCAGTGC CCCCTCCTTT GGGACTCTCG CCACTCAGAC TGCGCCGTCA 
TTCGGGAGCT TGGCCCAGCA GGGCCCTGGA TTTGGAAGCC AGCCCAGCAG CTTCTCAGGC 
TTTGGCCAGC AGCCACAGAC GGGAGGATTT TCAGGAAACG CATTTGGATC TCCAAATCCA 
CCGGGTGCTC CGTCGTTTGG CAGCTGGAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480026.1
CDS	148..6123
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGEKAVMERVAGVSFGHPRSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481724.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481724.2

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TGAGAAGGCT GTTATGGAGA GGGTTGCAGG TGTGAGTTTT 
GGTCATCCTC GTTCCGTGGC AACAGCACCA GCCAAGATTC CTGCTGCAGT TCCTTCTGCT 
CCACCTGCCT TCCCGAGCAT CAGCCTCGCT TCTTCAGCAG CTGCGCCCAG TCCTCCAGTC 
TCTTCTGCTC CGTCCTCCAT TATTCCTTTT AGCATGGCTC CAACGGCTCC TGTGACATCA 
GCCTTCTCCG GCTTTAGTTT CTCAGTCCCA TCAACCTTCT CCACCACCTC TGCCCCAGTT 
GCAGCTTTCG CCTTGGGAGG ATCTGTGCCT TTTGGCTCAG GGTCCTCTGG CTTCTCATTT 
GCCTCCAAAC CTCCGTCTGA GACCCCGTCC GCACTCTCGG CGTTTTCGTT TAATTCCCCT 
TCCGGCAAAC CACCAGCAGT GGCTGCCCCG TCTGCTGCTG TGGCACCTCA GAGCCTTTCA 
ACAGCCTCAC CGCCCACAGT CAAACTTAGT CTGAATGAAA GATTTTCAGG GGTGGAGGCT 
TCAGCTCCGG CTTTCTCTTT GCCTAAAGCT GTTGTTTCCA GCTCCAGTAG TTTCAATGTG 
CCTCCAATCT CAGCCTCAAA GCCGCCAGCT GTATGCGCTC CAGTGCACCC GGTGCAGACT 
AGTACACCTC CAGCCAGTGT CCAGAAACCT GCTCCTGCTG TCCAAGGACG TGTCTCTACT 
ATGCAGCAAG CCGTTAGCGT GAAAGCCCTG GAGAAACAGC TGCAGCAAAA GAAAGACTCT 
GATCCCATTA TGGCTGGAAT TTTGGAAGAG ATTGCACATT TTCAGAAAGA GTTAGATGAC 
CTGAAGGCAC GCAGTTCAAA AGCTGACGTC AGGGTCGGCA CAAACGATGA CATGAAGGAT 
CTGAGGAAAG AGTCTGAGGA CCTCCACATC TTCACTCTGG AGATTAAAGA AACGACAGAG 
TCTCTCCATG GTGACATTGG CACGCTGAAG ACCACATTAC TGGAGGGCTT TGCTGGGGCG 
GAAGAAGCCA AGACGCAGAA CGAGCTGGGA AAAGATAAAA ACTACAGACA GCTGTTGTAC 
AAAAAACCTC TGGATCCCAG AAGTGAGGAG CAACTCAAGG AGATTCGGAG GCTGTACCAG 
TACGTTAAAT TTGCTGTGGA GGATGTTAAC GACGTGTTGG ATGTGGAATG GGAGAAACAC 
CTGGAGAAAA AGAAAAAGCA AAAACACATG GTCGTGCCTG GACGTGAGGG TCTTTTCAAT 
ACATTGTCCA ACAACCTGTG CATCATCAAC CAGCAGAAGA ACCGACTGGA TCAGCTGATT 
CAGGAGCTCA GTGCTCTGCG GCTCTACAGC AAAACCACTG CTCCTACCAT TAACCAGAAC 
ACTGCAGCAA CATCAGATGA CTACAAAGGT GAGCTGGAGA ATTTGGAGGA GGCACTTCTG 
AAAGCCCGGC TTGAAACGAC TCCTCCTGAG ACAAAAGTGA AATCAGCAGT CAAGGTTTCT 
CCTCTTAAAC AGTCCCAACT GCGCAACTTC CTGTCAAAAG GGAAAATGCC TCCTGTGCGT 
TCAACTGCAC CAGCCAACTT GTCGCGCTCG GCCTTCCTGG CCCCTAAAAA CCTTGAGGAC 
TTGGATGATG TGAGCTCAAC GTCCTCCTTA TCGCAGTCTA TAGAGCTTCT CCCATCTCAC 
TTGGAGTTGG AGGAAGAGGA AGAACTACAG CCCCTGCAGC CTTCACCTAT GAATTCCATG 
ACCCAATCAC TTCCCACCCC ACGTCACCCC ACTGTTGTTC GAACGCCCTC CATTCAGCCA 
GGCTTTGGAG CTATACAGTC CACCCCTCTA ACAAAGATGC ACCCTGTTCC TGGTATGGGC 
TTTGTGCTAA GCCCCATTAT CAACCCTGTT CCTACAAATA GGATTAACCT CAGCGGGGCT 
GAAAGTACTG CGCTTGCCAC AAAGACGGTA AAGCATGGAG CACCACCTGC AGAGCGGAGC 
ATCCCTGTGA CCATCTCTGC CCAGCAGGCT GCTGCCACCG CGGCTCTGCG GAGACAAATG 
GCCAATCAGA AGACGGCTGT TGTCAACACT TCTTTGACAG AGTCCACCTT AAAAACTGTC 
CCTCAAGTAG TGAATGTTCA GGAGCTTAAG GACAAAGGGG CACCTATGCC AGTGTCCTCT 
ATGGTCAGCT CCTCGGCGCC AGATCCCTCA GTCCAGGGCT TCACAGCAGT TTCTAGCAGC 
CAAGTCAAAC GAAACACACC GGGTCTGCAA AAGATACCCA CAGAGAATGC ATCTATTCCA 
CAGACAGGCT TTGTCTTTGG CCAACCATCC AAAACAGACC TTTCCGGGGG TCCACCTAGT 
TCACTTGAGC AAAACACCAG CAAGGCCTTC TCATTCACCT CAGGATCGAC GGGCTTCAGT 
TTTTCATCTG TAACACAAGG TGCTGGATTG TCTCAAGGGA AGGATGTGAA CAAGTTCTCC 
ATTGGCGGAA ATGGCAAAGG GTTGTTTGGC CAAACTGGAG AGGAGCCATT CTCCCTTCCC 
ACTAAGTCAA ACTCTCCTGG TCTGAGCAAT GGGTCTTCAA GCTCATCGGC CAACAGTTTA 
GGGGAAGTAG CAAAACAGGG TTCCACTGTT ACTGGTTCTA AGACAGAGCC GCAGCCTCTA 
AAGGCAGCTG GAGGGGAGAC ATTAGGCAGT TTTTCAGGAC TACGAGTGGG ACACGGAGAA 
GAAGCAAACG AATCCTCCAC AAAGACCACT GCTCCTGCAT TTTCCTTCGG GAATGCTGGA 
CTTGGCCATA GCAAGTCAGC TCCTCAGTTT AACTTTGGTT CAACTCCCCT TAAGTCTGCC 
GAAGACGGGG CTGCAGCAGA CATGCCTAAG GTGTCAGGAT CAGGCAGCTT GTTTAAACCT 
CCTGATTCAA ACTCCATGCC AACCTTTTCT ATAACCCCTT CAACTACAGC TGCTTCAGCG 
CTGCCTACAT CTTTAAGTAG TCTTCTTGCA GCTCCTACTG AACAGCCTAA AGCTACTCAG 
CAACCTTCAG AAAGCACATC GGCTGCTTTA CCAGAACTGA GTACCAAGGA ACTTGCTGTA 
GACCTGGCTA GTGTTCCGGC AGAACCTGAG CCTCTAGTGA CTGCAGTTGC CACAACAACC 
ACGCCAACAT CTGCAGCACC AACACTTCCT ATGGTCACTC CTGTCCCTAC TGCAGACTCC 
TCTTCTACAG TTCCTGCAGA GACGGAAACA ACTCTGCCTC CAGCACCACC AACTGCTTCT 
TCCACAGAAG AAGCCACCCC AGACCCCTCT TCTACACCTG CCGTCTCCAC ATCTACCACC 
GCTGTCCCAC TTACCATCGA GGTAACCTCA GCAGCTTTAC CAGCTTCCTC CTCAGAAAAA 
CCAGGCTCCA TTTTTACCTC TGCTACTCCT GCAACCACCA CTAGCAATTC TGTTGGGGCC 
ATACCAGCCA TTAACACAGC TGCTCCCACA ACCACTACCG CTGCAGTTAT CACCAGTGCA 
GCAACGACAG CTGCTAATGT GTTTGGGCAG CCTGCCGCTC CTCCTGCCTC TTCTGCCCCT 
GCAACTACCG GATTCGGCTC ATCTGGATTT GGCGCACCAA CAGAAACCGG TTTCGGCAAA 
TCTGTATTTG GCGTGGGTGG GTTTGGACAG CCTGCTAGCA TAGGAGGAAC TTCGAGTGGC 
TTTTCATTCA CCCAGTCAGC ATTCGGAGCA AGTTCAAGCA GTGCGACCAC TGGAGGAGGT 
GGTCTTTTCG GTGCTCCCAC TGTTACCAAT GCAAGCTCCT TCTCCTTCGG CACCAGCAGT 
TCCAGCACAC CCAGCAGCAC CGGGCTGTTT AGCCAAAGCA CCCCGTCAGT ATTCGGCCAG 
AGCTCTGGAT TTGGGCAAGG ATCTATGTTC AGAAATAACA CCACCACATC ATCTGCGTCA 
GGCTTCAGCT TTGGACAGCC CTCAGGTTTT GGCTGTTCGG CGGCCACACC TGTGTTTGGC 
CAACAATCCA CCACTGGGAG CATATTTGGA CAGCAACAGC AACCTTCAAC TGGTGGAGGT 
TTGTTCGGTC AAAGTTCGAC CGGCGCTGCA ACCTCGTCCT CTGGCGGATT CTTCAGCGGG 
TTGGGAGGCA AACCCAGTGA AGACGCTGCC AACAAGAACC CATTTGGTAC CACCGCCTCC 
ACTGGCGTGT TTGGCCAGAG CACGCAGTCT GGGCCCAACA GCCTCTTTGG GAGCAGTAGC 
GCCAAGACCT TTGGATTTGG TCAGCCCTCC TTCGGGGAGC AGAAGCCCAG TGGGACCTTC 
AGTACTGGTG GAGGCAGTGT TGCATGTCAG GGCTTTGGTT CCTTCTCCAC ACCATCTAAA 
TCAACGAGCG GCTTTGGCAG CGCCCCCGTG TTCGGAAGCC CCCCTTCTTT TGGCGGTTCT 
CCTGCATTCG GTGGCGGCGC TTCATTCAGT TCAAGTCCGT CTTTCAGCAA CCCTTTAGCT 
TCCCAGTCCA GTAAAGTGTT TGGCGAGGGA ACAGCAGCCG CCAGCATGGG AGGATTTGGT 
TTCTCCTCAC CCCCCAGTGC CCCCTCCTTT GGGACTCTCG CCACTCAGAC TGCGCCGTCA 
TTCGGGAGCT TGGCCCAGCA GGGCCCTGGA TTTGGAAGCC AGCCCAGCAG CTTCTCAGGC 
TTTGGCCAGC AGCCACAGAC GGGAGGATTT TCAGGAAACG CATTTGGATC TCCAAATCCA 
CCGGGTGCTC CGTCGTTTGG CAGCTGGAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup214 ( XM_011481725.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011480027.1 Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.

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CloneID	OJa33583
Clone ID Related Accession (Same CDS sequence)	XM_011481725.2
Accession Version	XM_011481725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	nuclear pore complex protein Nup214 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019870.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011481725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TTCCGTGGCA ACAGCACCAG CCAAGATTCC TGCTGCAGTT 
CCTTCTGCTC CACCTGCCTT CCCGAGCATC AGCCTCGCTT CTTCAGCAGC TGCGCCCAGT 
CCTCCAGTCT CTTCTGCTCC GTCCTCCATT ATTCCTTTTA GCATGGCTCC AACGGCTCCT 
GTGACATCAG CCTTCTCCGG CTTTAGTTTC TCAGTCCCAT CAACCTTCTC CACCACCTCT 
GCCCCAGTTG CAGCTTTCGC CTTGGGAGGA TCTGTGCCTT TTGGCTCAGG GTCCTCTGGC 
TTCTCATTTG CCTCCAAACC TCCGTCTGAG ACCCCGTCCG CACTCTCGGC GTTTTCGTTT 
AATTCCCCTT CCGGCAAACC ACCAGCAGTG GCTGCCCCGT CTGCTGCTGT GGCACCTCAG 
AGCCTTTCAA CAGCCTCACC GCCCACAGTC AAACTTAGTC TGAATGAAAG ATTTTCAGGG 
GTGGAGGCTT CAGCTCCGGC TTTCTCTTTG CCTAAAGCTG TTGTTTCCAG CTCCAGTAGT 
TTCAATGTGC CTCCAATCTC AGCCTCAAAG CCGCCAGCTG TATGCGCTCC AGTGCACCCG 
GTGCAGACTA GTACACCTCC AGCCAGTGTC CAGAAACCTG CTCCTGCTGT CCAAGGACGT 
GTCTCTACTA TGCAGCAAGC CGTTAGCGTG AAAGCCCTGG AGAAACAGCT GCAGCAAAAG 
AAAGACTCTG ATCCCATTAT GGCTGGAATT TTGGAAGAGA TTGCACATTT TCAGAAAGAG 
TTAGATGACC TGAAGGCACG CAGTTCAAAA GCTGACGTCA GGGTCGGCAC AAACGATGAC 
ATGAAGGATC TGAGGAAAGA GTCTGAGGAC CTCCACATCT TCACTCTGGA GATTAAAGAA 
ACGACAGAGT CTCTCCATGG TGACATTGGC ACGCTGAAGA CCACATTACT GGAGGGCTTT 
GCTGGGGCGG AAGAAGCCAA GACGCAGAAC GAGCTGGGAA AAGATAAAAA CTACAGACAG 
CTGTTGTACA AAAAACCTCT GGATCCCAGA AGTGAGGAGC AACTCAAGGA GATTCGGAGG 
CTGTACCAGT ACGTTAAATT TGCTGTGGAG GATGTTAACG ACGTGTTGGA TGTGGAATGG 
GAGAAACACC TGGAGAAAAA GAAAAAGCAA AAACACATGG TCGTGCCTGG ACGTGAGGGT 
CTTTTCAATA CATTGTCCAA CAACCTGTGC ATCATCAACC AGCAGAAGAA CCGACTGGAT 
CAGCTGATTC AGGAGCTCAG TGCTCTGCGG CTCTACAGCA AAACCACTGC TCCTACCATT 
AACCAGAACA CTGCAGCAAC ATCAGATGAC TACAAAGGTG AGCTGGAGAA TTTGGAGGAG 
GCACTTCTGA AAGCCCGGCT TGAAACGACT CCTCCTGAGA CAAAAGTGAA ATCAGCAGCA 
GTCAAGGTTT CTCCTCTTAA ACAGTCCCAA CTGCGCAACT TCCTGTCAAA AGGGAAAATG 
CCTCCTGTGC GTTCAACTGC ACCAGCCAAC TTGTCGCGCT CGGCCTTCCT GGCCCCTAAA 
AACCTTGAGG ACTTGGATGA TGTGAGCTCA ACGTCCTCCT TATCGCAGTC TATAGAGCTT 
CTCCCATCTC ACTTGGAGTT GGAGGAAGAG GAAGAACTAC AGCCCCTGCA GCCTTCACCT 
ATGAATTCCA TGACCCAATC ACTTCCCACC CCACGTCACC CCACTGTTGT TCGAACGCCC 
TCCATTCAGC CAGGCTTTGG AGCTATACAG TCCACCCCTC TAACAAAGAT GCACCCTGTT 
CCTGGTATGG GCTTTGTGCT AAGCCCCATT ATCAACCCTG TTCCTACAAA TAGGATTAAC 
CTCAGCGGGG CTGAAAGTAC TGCGCTTGCC ACAAAGACGG TAAAGCATGG AGCACCACCT 
GCAGAGCGGA GCATCCCTGT GACCATCTCT GCCCAGCAGG CTGCTGCCAC CGCGGCTCTG 
CGGAGACAAA TGGCCAATCA GAAGACGGCT GTTGTCAACA CTTCTTTGAC AGAGTCCACC 
TTAAAAACTG TCCCTCAAGT AGTGAATGTT CAGGAGCTTA AGGACAAAGG GGCACCTATG 
CCAGTGTCCT CTATGGTCAG CTCCTCGGCG CCAGATCCCT CAGTCCAGGG CTTCACAGCA 
GTTTCTAGCA GCCAAGTCAA ACGAAACACA CCGGGTCTGC AAAAGATACC CACAGAGAAT 
GCATCTATTC CACAGACAGG CTTTGTCTTT GGCCAACCAT CCAAAACAGA CCTTTCCGGG 
GGTCCACCTA GTTCACTTGA GCAAAACACC AGCAAGGCCT TCTCATTCAC CTCAGGATCG 
ACGGGCTTCA GTTTTTCATC TGTAACACAA GGTGCTGGAT TGTCTCAAGG GAAGGATGTG 
AACAAGTTCT CCATTGGCGG AAATGGCAAA GGGTTGTTTG GCCAAACTGG AGAGGAGCCA 
TTCTCCCTTC CCACTAAGTC AAACTCTCCT GGTCTGAGCA ATGGGTCTTC AAGCTCATCG 
GCCAACAGTT TAGGGGAAGT AGCAAAACAG GGTTCCACTG TTACTGGTTC TAAGACAGAG 
CCGCAGCCTC TAAAGGCAGC TGGAGGGGAG ACATTAGGCA GTTTTTCAGG ACTACGAGTG 
GGACACGGAG AAGAAGCAAA CGAATCCTCC ACAAAGACCA CTGCTCCTGC ATTTTCCTTC 
GGGAATGCTG GACTTGGCCA TAGCAAGTCA GCTCCTCAGT TTAACTTTGG TTCAACTCCC 
CTTAAGTCTG CCGAAGACGG GGCTGCAGCA GACATGCCTA AGGTGTCAGG ATCAGGCAGC 
TTGTTTAAAC CTCCTGATTC AAACTCCATG CCAACCTTTT CTATAACCCC TTCAACTACA 
GCTGCTTCAG CGCTGCCTAC ATCTTTAAGT AGTCTTCTTG CAGCTCCTAC TGAACAGCCT 
AAAGCTACTC AGCAACCTTC AGAAAGCACA TCGGCTGCTT TACCAGAACT GAGTACCAAG 
GAACTTGCTG TAGACCTGGC TAGTGTTCCG GCAGAACCTG AGCCTCTAGT GACTGCAGTT 
GCCACAACAA CCACGCCAAC ATCTGCAGCA CCAACACTTC CTATGGTCAC TCCTGTCCCT 
ACTGCAGACT CCTCTTCTAC AGTTCCTGCA GAGACGGAAA CAACTCTGCC TCCAGCACCA 
CCAACTGCTT CTTCCACAGA AGAAGCCACC CCAGACCCCT CTTCTACACC TGCCGTCTCC 
ACATCTACCA CCGCTGTCCC ACTTACCATC GAGGTAACCT CAGCAGCTTT ACCAGCTTCC 
TCCTCAGAAA AACCAGGCTC CATTTTTACC TCTGCTACTC CTGCAACCAC CACTAGCAAT 
TCTGTTGGGG CCATACCAGC CATTAACACA GCTGCTCCCA CAACCACTAC CGCTGCAGTT 
ATCACCAGTG CAGCAACGAC AGCTGCTAAT GTGTTTGGGC AGCCTGCCGC TCCTCCTGCC 
TCTTCTGCCC CTGCAACTAC CGGATTCGGC TCATCTGGAT TTGGCGCACC AACAGAAACC 
GGTTTCGGCA AATCTGTATT TGGCGTGGGT GGGTTTGGAC AGCCTGCTAG CATAGGAGGA 
ACTTCGAGTG GCTTTTCATT CACCCAGTCA GCATTCGGAG CAAGTTCAAG CAGTGCGACC 
ACTGGAGGAG GTGGTCTTTT CGGTGCTCCC ACTGTTACCA ATGCAAGCTC CTTCTCCTTC 
GGCACCAGCA GTTCCAGCAC ACCCAGCAGC ACCGGGCTGT TTAGCCAAAG CACCCCGTCA 
GTATTCGGCC AGAGCTCTGG ATTTGGGCAA GGATCTATGT TCAGAAATAA CACCACCACA 
TCATCTGCGT CAGGCTTCAG CTTTGGACAG CCCTCAGGTT TTGGCTGTTC GGCGGCCACA 
CCTGTGTTTG GCCAACAATC CACCACTGGG AGCATATTTG GACAGCAACA GCAACCTTCA 
ACTGGTGGAG GTTTGTTCGG TCAAAGTTCG ACCGGCGCTG CAACCTCGTC CTCTGGCGGA 
TTCTTCAGCG GGTTGGGAGG CAAACCCAGT GAAGACGCTG CCAACAAGAA CCCATTTGGT 
ACCACCGCCT CCACTGGCGT GTTTGGCCAG AGCACGCAGT CTGGGCCCAA CAGCCTCTTT 
GGGAGCAGTA GCGCCAAGAC CTTTGGATTT GGTCAGCCCT CCTTCGGGGA GCAGAAGCCC 
AGTGGGACCT TCAGTACTGG TGGAGGCAGT GTTGCATGTC AGGGCTTTGG TTCCTTCTCC 
ACACCATCTA AATCAACGAG CGGCTTTGGC AGCGCCCCCG TGTTCGGAAG CCCCCCTTCT 
TTTGGCGGTT CTCCTGCATT CGGTGGCGGC GCTTCATTCA GTTCAAGTCC GTCTTTCAGC 
AACCCTTTAG CTTCCCAGTC CAGTAAAGTG TTTGGCGAGG GAACAGCAGC CGCCAGCATG 
GGAGGATTTG GTTTCTCCTC ACCCCCCAGT GCCCCCTCCT TTGGGACTCT CGCCACTCAG 
ACTGCGCCGT CATTCGGGAG CTTGGCCCAG CAGGGCCCTG GATTTGGAAG CCAGCCCAGC 
AGCTTCTCAG GCTTTGGCCA GCAGCCACAG ACGGGAGGAT TTTCAGGAAA CGCATTTGGA 
TCTCCAAATC CACCGGGTGC TCCGTCGTTT GGCAGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011480027.1
CDS	148..6075
Translation	MSDDADSPPERLMKDFQFRQLKKVEVFEPPEHLPKDRSSLLTVSNKYGLTFVGLERTFKVYQTQDILSADKADPGFIVKGTPPLATVTVELALHHLALSCDELTLSVCGVEEENLFLTFYDVRTFINGKSTQKLPFATFRPDVPPGTLVQDLKWNPANVSMLAACLSDGSMMILDVTDLVKMQAKLPSTSGITCIGWSPKGKQVAAGKMNGTVSQYTPALEEKKVIPCPHFYTSEDPVKALDVLWLRTFAFAVTYAAADGSLETPPELVLVSLPKKDEKVDTKYLNFGDIVYGTCTERQHHYFLSHIEDWDLVFAASAASIEVSVIARQDDKMWELWILEDAGRAELRVTESNEDTLPLGLAIDYTSQQEIYITDEKTLPPAPTLMMLSTEGLLCSFALVNQNPGAKQLVSPPTMLTPEGERLPKPGSVATAPAKIPAAVPSAPPAFPSISLASSAAAPSPPVSSAPSSIIPFSMAPTAPVTSAFSGFSFSVPSTFSTTSAPVAAFALGGSVPFGSGSSGFSFASKPPSETPSALSAFSFNSPSGKPPAVAAPSAAVAPQSLSTASPPTVKLSLNERFSGVEASAPAFSLPKAVVSSSSSFNVPPISASKPPAVCAPVHPVQTSTPPASVQKPAPAVQGRVSTMQQAVSVKALEKQLQQKKDSDPIMAGILEEIAHFQKELDDLKARSSKADVRVGTNDDMKDLRKESEDLHIFTLEIKETTESLHGDIGTLKTTLLEGFAGAEEAKTQNELGKDKNYRQLLYKKPLDPRSEEQLKEIRRLYQYVKFAVEDVNDVLDVEWEKHLEKKKKQKHMVVPGREGLFNTLSNNLCIINQQKNRLDQLIQELSALRLYSKTTAPTINQNTAATSDDYKGELENLEEALLKARLETTPPETKVKSAAVKVSPLKQSQLRNFLSKGKMPPVRSTAPANLSRSAFLAPKNLEDLDDVSSTSSLSQSIELLPSHLELEEEEELQPLQPSPMNSMTQSLPTPRHPTVVRTPSIQPGFGAIQSTPLTKMHPVPGMGFVLSPIINPVPTNRINLSGAESTALATKTVKHGAPPAERSIPVTISAQQAAATAALRRQMANQKTAVVNTSLTESTLKTVPQVVNVQELKDKGAPMPVSSMVSSSAPDPSVQGFTAVSSSQVKRNTPGLQKIPTENASIPQTGFVFGQPSKTDLSGGPPSSLEQNTSKAFSFTSGSTGFSFSSVTQGAGLSQGKDVNKFSIGGNGKGLFGQTGEEPFSLPTKSNSPGLSNGSSSSSANSLGEVAKQGSTVTGSKTEPQPLKAAGGETLGSFSGLRVGHGEEANESSTKTTAPAFSFGNAGLGHSKSAPQFNFGSTPLKSAEDGAAADMPKVSGSGSLFKPPDSNSMPTFSITPSTTAASALPTSLSSLLAAPTEQPKATQQPSESTSAALPELSTKELAVDLASVPAEPEPLVTAVATTTTPTSAAPTLPMVTPVPTADSSSTVPAETETTLPPAPPTASSTEEATPDPSSTPAVSTSTTAVPLTIEVTSAALPASSSEKPGSIFTSATPATTTSNSVGAIPAINTAAPTTTTAAVITSAATTAANVFGQPAAPPASSAPATTGFGSSGFGAPTETGFGKSVFGVGGFGQPASIGGTSSGFSFTQSAFGASSSSATTGGGGLFGAPTVTNASSFSFGTSSSSTPSSTGLFSQSTPSVFGQSSGFGQGSMFRNNTTTSSASGFSFGQPSGFGCSAATPVFGQQSTTGSIFGQQQQPSTGGGLFGQSSTGAATSSSGGFFSGLGGKPSEDAANKNPFGTTASTGVFGQSTQSGPNSLFGSSSAKTFGFGQPSFGEQKPSGTFSTGGGSVACQGFGSFSTPSKSTSGFGSAPVFGSPPSFGGSPAFGGGASFSSSPSFSNPLASQSSKVFGEGTAAASMGGFGFSSPPSAPSFGTLATQTAPSFGSLAQQGPGFGSQPSSFSGFGQQPQTGGFSGNAFGSPNPPGAPSFGSWRS

Target ORF information:

RefSeq Version	XM_011481725.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes nucleoporin 214 (nup214), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481725.2

ORF Insert Sequence:

ATGAGTGACG ATGCAGACTC TCCTCCTGAG AGGTTAATGA AGGACTTTCA GTTTCGTCAA 
TTGAAGAAGG TTGAAGTTTT TGAGCCGCCG GAACATTTGC CTAAAGACAG ATCTAGTCTT 
CTTACTGTAT CTAACAAATA CGGATTAACA TTCGTGGGAC TCGAGAGAAC ATTTAAAGTC 
TACCAAACTC AAGATATTTT GAGTGCTGAT AAAGCGGATC CTGGTTTCAT AGTCAAGGGC 
ACACCACCAT TAGCTACAGT GACTGTGGAA CTGGCTCTGC ATCATTTGGC TCTCAGCTGT 
GATGAACTTA CATTGTCAGT TTGCGGTGTG GAAGAGGAGA ATTTGTTTCT GACATTTTAT 
GATGTTCGCA CCTTCATCAA TGGGAAATCA ACACAGAAGT TGCCTTTTGC AACCTTTCGG 
CCAGATGTGC CACCTGGCAC TTTGGTGCAG GACCTGAAGT GGAATCCAGC GAATGTCTCT 
ATGCTGGCTG CTTGTCTGTC TGATGGCAGC ATGATGATTC TGGATGTTAC AGATCTTGTG 
AAAATGCAGG CTAAGCTGCC CTCCACAAGT GGCATCACAT GCATCGGTTG GAGTCCAAAA 
GGAAAGCAGG TTGCTGCAGG AAAGATGAAT GGCACTGTTA GTCAATATAC ACCGGCACTG 
GAAGAAAAAA AAGTCATCCC GTGTCCACAC TTCTACACCT CTGAGGACCC TGTGAAGGCT 
CTGGATGTTC TCTGGCTGAG AACATTTGCA TTCGCAGTGA CATATGCTGC TGCAGATGGG 
TCTTTAGAGA CCCCTCCTGA GCTTGTGTTG GTCTCCCTGC CTAAAAAGGA TGAAAAGGTG 
GATACCAAGT ATTTGAATTT TGGTGACATA GTGTACGGCA CCTGCACCGA ACGACAACAT 
CACTACTTCC TAAGTCATAT AGAGGACTGG GACCTTGTTT TTGCAGCGTC AGCCGCTTCT 
ATTGAGGTCA GCGTCATTGC AAGACAAGAT GACAAGATGT GGGAACTGTG GATCCTTGAA 
GATGCGGGTA GGGCTGAACT ACGAGTGACC GAGTCAAATG AAGACACTCT GCCTCTTGGT 
TTGGCAATAG ACTACACCAG CCAGCAGGAG ATCTACATTA CCGATGAGAA GACCTTACCT 
CCAGCACCCA CTCTGATGAT GCTGTCCACA GAGGGATTGC TTTGCTCATT TGCTTTAGTC 
AACCAAAACC CTGGGGCCAA ACAGCTTGTC TCACCCCCTA CAATGCTGAC CCCTGAAGGG 
GAGAGGCTAC CAAAGCCAGG TTCCGTGGCA ACAGCACCAG CCAAGATTCC TGCTGCAGTT 
CCTTCTGCTC CACCTGCCTT CCCGAGCATC AGCCTCGCTT CTTCAGCAGC TGCGCCCAGT 
CCTCCAGTCT CTTCTGCTCC GTCCTCCATT ATTCCTTTTA GCATGGCTCC AACGGCTCCT 
GTGACATCAG CCTTCTCCGG CTTTAGTTTC TCAGTCCCAT CAACCTTCTC CACCACCTCT 
GCCCCAGTTG CAGCTTTCGC CTTGGGAGGA TCTGTGCCTT TTGGCTCAGG GTCCTCTGGC 
TTCTCATTTG CCTCCAAACC TCCGTCTGAG ACCCCGTCCG CACTCTCGGC GTTTTCGTTT 
AATTCCCCTT CCGGCAAACC ACCAGCAGTG GCTGCCCCGT CTGCTGCTGT GGCACCTCAG 
AGCCTTTCAA CAGCCTCACC GCCCACAGTC AAACTTAGTC TGAATGAAAG ATTTTCAGGG 
GTGGAGGCTT CAGCTCCGGC TTTCTCTTTG CCTAAAGCTG TTGTTTCCAG CTCCAGTAGT 
TTCAATGTGC CTCCAATCTC AGCCTCAAAG CCGCCAGCTG TATGCGCTCC AGTGCACCCG 
GTGCAGACTA GTACACCTCC AGCCAGTGTC CAGAAACCTG CTCCTGCTGT CCAAGGACGT 
GTCTCTACTA TGCAGCAAGC CGTTAGCGTG AAAGCCCTGG AGAAACAGCT GCAGCAAAAG 
AAAGACTCTG ATCCCATTAT GGCTGGAATT TTGGAAGAGA TTGCACATTT TCAGAAAGAG 
TTAGATGACC TGAAGGCACG CAGTTCAAAA GCTGACGTCA GGGTCGGCAC AAACGATGAC 
ATGAAGGATC TGAGGAAAGA GTCTGAGGAC CTCCACATCT TCACTCTGGA GATTAAAGAA 
ACGACAGAGT CTCTCCATGG TGACATTGGC ACGCTGAAGA CCACATTACT GGAGGGCTTT 
GCTGGGGCGG AAGAAGCCAA GACGCAGAAC GAGCTGGGAA AAGATAAAAA CTACAGACAG 
CTGTTGTACA AAAAACCTCT GGATCCCAGA AGTGAGGAGC AACTCAAGGA GATTCGGAGG 
CTGTACCAGT ACGTTAAATT TGCTGTGGAG GATGTTAACG ACGTGTTGGA TGTGGAATGG 
GAGAAACACC TGGAGAAAAA GAAAAAGCAA AAACACATGG TCGTGCCTGG ACGTGAGGGT 
CTTTTCAATA CATTGTCCAA CAACCTGTGC ATCATCAACC AGCAGAAGAA CCGACTGGAT 
CAGCTGATTC AGGAGCTCAG TGCTCTGCGG CTCTACAGCA AAACCACTGC TCCTACCATT 
AACCAGAACA CTGCAGCAAC ATCAGATGAC TACAAAGGTG AGCTGGAGAA TTTGGAGGAG 
GCACTTCTGA AAGCCCGGCT TGAAACGACT CCTCCTGAGA CAAAAGTGAA ATCAGCAGCA 
GTCAAGGTTT CTCCTCTTAA ACAGTCCCAA CTGCGCAACT TCCTGTCAAA AGGGAAAATG 
CCTCCTGTGC GTTCAACTGC ACCAGCCAAC TTGTCGCGCT CGGCCTTCCT GGCCCCTAAA 
AACCTTGAGG ACTTGGATGA TGTGAGCTCA ACGTCCTCCT TATCGCAGTC TATAGAGCTT 
CTCCCATCTC ACTTGGAGTT GGAGGAAGAG GAAGAACTAC AGCCCCTGCA GCCTTCACCT 
ATGAATTCCA TGACCCAATC ACTTCCCACC CCACGTCACC CCACTGTTGT TCGAACGCCC 
TCCATTCAGC CAGGCTTTGG AGCTATACAG TCCACCCCTC TAACAAAGAT GCACCCTGTT 
CCTGGTATGG GCTTTGTGCT AAGCCCCATT ATCAACCCTG TTCCTACAAA TAGGATTAAC 
CTCAGCGGGG CTGAAAGTAC TGCGCTTGCC ACAAAGACGG TAAAGCATGG AGCACCACCT 
GCAGAGCGGA GCATCCCTGT GACCATCTCT GCCCAGCAGG CTGCTGCCAC CGCGGCTCTG 
CGGAGACAAA TGGCCAATCA GAAGACGGCT GTTGTCAACA CTTCTTTGAC AGAGTCCACC 
TTAAAAACTG TCCCTCAAGT AGTGAATGTT CAGGAGCTTA AGGACAAAGG GGCACCTATG 
CCAGTGTCCT CTATGGTCAG CTCCTCGGCG CCAGATCCCT CAGTCCAGGG CTTCACAGCA 
GTTTCTAGCA GCCAAGTCAA ACGAAACACA CCGGGTCTGC AAAAGATACC CACAGAGAAT 
GCATCTATTC CACAGACAGG CTTTGTCTTT GGCCAACCAT CCAAAACAGA CCTTTCCGGG 
GGTCCACCTA GTTCACTTGA GCAAAACACC AGCAAGGCCT TCTCATTCAC CTCAGGATCG 
ACGGGCTTCA GTTTTTCATC TGTAACACAA GGTGCTGGAT TGTCTCAAGG GAAGGATGTG 
AACAAGTTCT CCATTGGCGG AAATGGCAAA GGGTTGTTTG GCCAAACTGG AGAGGAGCCA 
TTCTCCCTTC CCACTAAGTC AAACTCTCCT GGTCTGAGCA ATGGGTCTTC AAGCTCATCG 
GCCAACAGTT TAGGGGAAGT AGCAAAACAG GGTTCCACTG TTACTGGTTC TAAGACAGAG 
CCGCAGCCTC TAAAGGCAGC TGGAGGGGAG ACATTAGGCA GTTTTTCAGG ACTACGAGTG 
GGACACGGAG AAGAAGCAAA CGAATCCTCC ACAAAGACCA CTGCTCCTGC ATTTTCCTTC 
GGGAATGCTG GACTTGGCCA TAGCAAGTCA GCTCCTCAGT TTAACTTTGG TTCAACTCCC 
CTTAAGTCTG CCGAAGACGG GGCTGCAGCA GACATGCCTA AGGTGTCAGG ATCAGGCAGC 
TTGTTTAAAC CTCCTGATTC AAACTCCATG CCAACCTTTT CTATAACCCC TTCAACTACA 
GCTGCTTCAG CGCTGCCTAC ATCTTTAAGT AGTCTTCTTG CAGCTCCTAC TGAACAGCCT 
AAAGCTACTC AGCAACCTTC AGAAAGCACA TCGGCTGCTT TACCAGAACT GAGTACCAAG 
GAACTTGCTG TAGACCTGGC TAGTGTTCCG GCAGAACCTG AGCCTCTAGT GACTGCAGTT 
GCCACAACAA CCACGCCAAC ATCTGCAGCA CCAACACTTC CTATGGTCAC TCCTGTCCCT 
ACTGCAGACT CCTCTTCTAC AGTTCCTGCA GAGACGGAAA CAACTCTGCC TCCAGCACCA 
CCAACTGCTT CTTCCACAGA AGAAGCCACC CCAGACCCCT CTTCTACACC TGCCGTCTCC 
ACATCTACCA CCGCTGTCCC ACTTACCATC GAGGTAACCT CAGCAGCTTT ACCAGCTTCC 
TCCTCAGAAA AACCAGGCTC CATTTTTACC TCTGCTACTC CTGCAACCAC CACTAGCAAT 
TCTGTTGGGG CCATACCAGC CATTAACACA GCTGCTCCCA CAACCACTAC CGCTGCAGTT 
ATCACCAGTG CAGCAACGAC AGCTGCTAAT GTGTTTGGGC AGCCTGCCGC TCCTCCTGCC 
TCTTCTGCCC CTGCAACTAC CGGATTCGGC TCATCTGGAT TTGGCGCACC AACAGAAACC 
GGTTTCGGCA AATCTGTATT TGGCGTGGGT GGGTTTGGAC AGCCTGCTAG CATAGGAGGA 
ACTTCGAGTG GCTTTTCATT CACCCAGTCA GCATTCGGAG CAAGTTCAAG CAGTGCGACC 
ACTGGAGGAG GTGGTCTTTT CGGTGCTCCC ACTGTTACCA ATGCAAGCTC CTTCTCCTTC 
GGCACCAGCA GTTCCAGCAC ACCCAGCAGC ACCGGGCTGT TTAGCCAAAG CACCCCGTCA 
GTATTCGGCC AGAGCTCTGG ATTTGGGCAA GGATCTATGT TCAGAAATAA CACCACCACA 
TCATCTGCGT CAGGCTTCAG CTTTGGACAG CCCTCAGGTT TTGGCTGTTC GGCGGCCACA 
CCTGTGTTTG GCCAACAATC CACCACTGGG AGCATATTTG GACAGCAACA GCAACCTTCA 
ACTGGTGGAG GTTTGTTCGG TCAAAGTTCG ACCGGCGCTG CAACCTCGTC CTCTGGCGGA 
TTCTTCAGCG GGTTGGGAGG CAAACCCAGT GAAGACGCTG CCAACAAGAA CCCATTTGGT 
ACCACCGCCT CCACTGGCGT GTTTGGCCAG AGCACGCAGT CTGGGCCCAA CAGCCTCTTT 
GGGAGCAGTA GCGCCAAGAC CTTTGGATTT GGTCAGCCCT CCTTCGGGGA GCAGAAGCCC 
AGTGGGACCT TCAGTACTGG TGGAGGCAGT GTTGCATGTC AGGGCTTTGG TTCCTTCTCC 
ACACCATCTA AATCAACGAG CGGCTTTGGC AGCGCCCCCG TGTTCGGAAG CCCCCCTTCT 
TTTGGCGGTT CTCCTGCATT CGGTGGCGGC GCTTCATTCA GTTCAAGTCC GTCTTTCAGC 
AACCCTTTAG CTTCCCAGTC CAGTAAAGTG TTTGGCGAGG GAACAGCAGC CGCCAGCATG 
GGAGGATTTG GTTTCTCCTC ACCCCCCAGT GCCCCCTCCT TTGGGACTCT CGCCACTCAG 
ACTGCGCCGT CATTCGGGAG CTTGGCCCAG CAGGGCCCTG GATTTGGAAG CCAGCCCAGC 
AGCTTCTCAG GCTTTGGCCA GCAGCCACAG ACGGGAGGAT TTTCAGGAAA CGCATTTGGA 
TCTCCAAATC CACCGGGTGC TCCGTCGTTT GGCAGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nup214 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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