RYR1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	RYR1
Entrez Gene ID	100009540
Full Name	ryanodine receptor 1
Synonyms	RYR-1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101718.1	NP_001095188.1	ryanodine receptor 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Ion-pulling simulations provide insights into the mechanisms of channel opening of the skeletal muscle ryanodine receptor.
Mowrey DD, Xu L, Mei Y, Pasek DA, Meissner G, Dokholyan NV
The Journal of biological chemistry292(31)12947-12958(2017 08)

Reduced threshold for store overload-induced Ca
Chen W, Koop A, Liu Y, Guo W, Wei J, Wang R, MacLennan DH, Dirksen RT, Chen SRW
The Biochemical journal474(16)2749-2761(2017 08)

The Central domain of RyR1 is the transducer for long-range allosteric gating of channel opening.
Bai XC, Yan Z, Wu J, Li Z, Yan N
Cell research26(9)995-1006(2016 09)

Structural insights into Ca(2+)-activated long-range allosteric channel gating of RyR1.
Wei R, Wang X, Zhang Y, Mukherjee S, Zhang L, Chen Q, Huang X, Jing S, Liu C, Li S, Wang G, Xu Y, Zhu S, Williams AJ, Sun F, Yin CC
Cell research26(9)977-94(2016 09)

Channel Gating Dependence on Pore Lining Helix Glycine Residues in Skeletal Muscle Ryanodine Receptor.
Mei Y, Xu L, Mowrey DD, Mendez Giraldez R, Wang Y, Pasek DA, Dokholyan NV, Meissner G
The Journal of biological chemistry290(28)17535-45(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RYR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RYR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00344	NM_001101718.1 Latest version!	Oryctolagus cuniculus ryanodine receptor 1 (RYR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RYR1 ( NM_001101718.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001095188.1 Oryctolagus cuniculus ryanodine receptor 1 (RYR1), mRNA.

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CloneID	OOb00344
Clone ID Related Accession (Same CDS sequence)	NM_001101718.1
Accession Version	NM_001101718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Oryctolagus cuniculus(rabbit)
Product	ryanodine receptor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X15209.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876825, SAMEA1876880 [ECO:0000350] ##Evidence-Data-END##

ATGGGTGACG GAGGAGAGGG CGAGGACGAA GTCCAGTTCC TGCGGACGGA CGACGAGGTG 
GTCCTGCAGT GCAGCGCCAC CGTCCTCAAG GAGCAGCTGA AGCTGTGCCT GGCCGCCGAG 
GGCTTCGGCA ACCGCCTCTG CTTCCTGGAG CCCACCAGCA ACGCCCAGAA TGTGCCCCCC 
GACCTGGCCA TCTGCTGCTT CACCCTGGAG CAGTCCCTGT CGGTCCGCGC CCTGCAGGAG 
ATGCTGGCCA ACACCGTGGA GGCTGGCGTG GAGTCATCCC AGGGCGGGGG ACACAGGACA 
CTCCTGTATG GCCATGCCAT CCTGCTCCGG CATGCACACA GCCGCATGTA CCTGAGCTGC 
CTCACTACCT CGCGCTCCAT GACTGACAAG CTGGCTTTTG ACGTGGGACT GCAGGAGGAT 
GCAACAGGAG AGGCCTGTTG GTGGACCATG CACCCAGCCT CCAAGCAGAG ATCGGAAGGA 
GAGAAGGTCC GCGTCGGGGA CGACCTCATC CTTGTCAGCG TCTCCTCCGA GCGCTACCTG 
CACCTGTCGA CGGCCAGTGG GGAGCTCCAG GTTGATGCCT CCTTCATGCA GACCCTGTGG 
AACATGAACC CCATCTGCTC CTGCTGTGAA GAGGGCTACG TGACTGGGGG CCACGTCCTC 
CGCCTCTTCC ACGGACACAT GGACGAGTGC TTGACTATTT CTGCTGCTGA CAGCGACGAC 
CAGCGCAGGC TTGTCTACTA CGAGGGGGGA GCTGTGTGCA CGCACGCCCG CTCCCTCTGG 
AGGCTGGAAC CCCTGAGAAT CAGCTGGAGT GGAAGCCACC TGCGCTGGGG CCAGCCACTG 
CGGATCCGGC ATGTCACCAC CGGACGGTAC CTGGCACTCA CCGAGGACCA GGGCCTGGTG 
GTGGTCGATG CCTGCAAGGC CCACACCAAG GCCACCTCCT TCTGTTTCCG TGTCTCCAAG 
GAGAAGCTGG ACACGGCCCC CAAGCGAGAC GTGGAGGGCA TGGGCCCGCC TGAGATCAAG 
TACGGGGAGT CGCTGTGCTT TGTGCAGCAC GTGGCCTCGG GCCTGTGGCT CACCTACGCC 
GCCCCTGACC CCAAGGCCCT GCGGCTGGGC GTGCTGAAGA AGAAGGCCAT TCTGCACCAG 
GAGGGCCACA TGGACGATGC ACTGTTCCTG ACCCGCTGCC AGCAGGAGGA GTCCCAGGCT 
GCCCGCATGA TCCACAGCAC TGCCGGCCTG TACAACCAGT TCATCAAGGG CCTGGACAGC 
TTCAGCGGGA AGCCCCGGGG CTCAGGGCCG CCGGCTGGCC CTGCGCTGCC CATCGAGGCC 
GTCATCCTGA GCCTGCAGGA CCTCATTGGC TACTTCGAGC CGCCCTCGGA GGAGCTGCAG 
CACGAGGAAA AGCAGAGCAA GCTGCGCAGC CTGCGCAACC GCCAGAGCCT CTTCCAGGAG 
GAGGGGATGC TCTCCCTGGT CCTGAACTGC ATCGACCGCC TGAACGTCTA CACCACCGCT 
GCCCACTTTG CCGAGTATGC AGGGGAAGAG GCAGCTGAGT CCTGGAAAGA GATTGTTAAC 
CTACTCTACG AACTCCTGGC CTCTCTGATC CGGGGCAATC GTGCCAACTG TGCCCTCTTC 
TCCACAAACT TGGACTGGGT GGTCAGCAAA CTGGACCGGC TGGAGGCCTC GTCTGGCATC 
CTGGAGGTGC TGTACTGTGT CCTGATTGAG AGTCCCGAGG TGCTGAACAT CATCCAGGAG 
AACCACATCA AGTCCATCAT CTCCCTCCTG GACAAGCATG GGAGGAACCA CAAGGTTCTG 
GATGTGCTGT GCTCCCTGTG TGTGTGCAAT GGCGTGGCTG TGCGCTCCAA CCAAGATCTC 
ATCACCGAGA ATCTGCTCCC TGGCCGTGAG CTGCTGCTGC AGACAAACCT TATCAACTAC 
GTCACCAGCA TCCGCCCCAA CATCTTCGTG GGCCGAGCCG AGGGCTCCAC GCAGTATGGC 
AAATGGTACT TTGAGGTGAT GGTGGACGAG GTGGTTCCAT TTCTGACAGC TCAGGCCACC 
CATCTGCGGG TGGGCTGGGC CCTCACCGAG GGCTACAGCC CCTACCCTGG CGGCGGCGAG 
GGCTGGGGCG GCAATGGGGT CGGCGATGAC CTCTACTCCT ATGGCTTTGA CGGGCTGCAC 
CTCTGGACAG GACACGTGGC ACGCCCGGTG ACCTCCCCCG GGCAGCACCT CCTGGCCCCT 
GAGGACGTGG TCAGCTGCTG CCTGGACCTC AGCGTGCCGT CCATCTCCTT CCGCATCAAT 
GGCTGCCCCG TGCAGGGTGT CTTTGAGGCC TTCAACCTGG ATGGGCTCTT CTTCCCTGTG 
GTCAGCTTCT CGGCTGGTGT CAAGGTGCGG TTTCTCCTTG GCGGCCGCCA TGGTGAATTC 
AAGTTCCTGC CCCCACCCGG CTACGCCCCA TGCCATGAGG CAGTGCTCCC GCGAGAGCGA 
CTCCGTCTTG AGCCCATCAA GGAGTACAGA CGGGAAGGGC CGCGGGGGCC CCACCTGGTG 
GGCCCCAGCC GCTGCCTCTC GCACACTGAC TTTGTGCCCT GCCCGGTGGA CACCGTCCAG 
ATCGTCCTGC CTCCCCACCT GGAGCGCATC CGTGAGAAGC TGGCAGAGAA CATCCATGAA 
CTCTGGGCGC TGACGCGCAT TGAGCAAGGC TGGACCTATG GCCCGGTTCG GGATGACAAC 
AAGAGGCTGC ACCCGTGTCT TGTGAACTTC CACAGCCTTC CGGAGCCCGA GAGGAATTAC 
AACCTGCAGA TGTCGGGCGA GACTCTCAAG ACTCTGCTGG CGCTGGGCTG CCACGTGGGC 
ATGGCCGACG AGAAGGCGGA GGACAACCTG AAGAAGACAA AGCTGCCCAA GACGTATATG 
ATGAGCAATG GGTACAAGCC AGCCCCGCTG GACTTGAGCC ATGTGCGACT GACACCCGCA 
CAGACGACAC TGGTGGACCG GCTGGCAGAG AATGGGCACA ACGTGTGGGC ACGAGACCGA 
GTGGCCCAGG GCTGGAGCTA CAGCGCCGTG CAGGACATCC CGGCTCGCCG GAACCCTCGC 
CTTGTGCCCT ACCGCCTGCT GGACGAGGCC ACCAAGCGCA GCAACCGGGA CAGCCTCTGC 
CAGGCCGTGC GCACCCTGCT GGGCTATGGC TACAACATCG AGCCACCTGA CCAGGAGCCC 
AGTCAGGTGG AGAACCAGTC TCGCTGGGAC CGGGTGCGCA TCTTCCGGGC AGAGAAATCC 
TACACAGTGC AGAGTGGCCG CTGGTACTTC GAGTTTGAGG CCGTCACCAC AGGCGAGATG 
CGAGTGGGCT GGGCGAGGCC CGAGCTGCGG CCTGACGTCG AGCTGGGAGC GGATGAGCTG 
GCCTACGTCT TCAACGGGCA CCGTGGTCAG CGCTGGCACT TGGGCAGTGA GCCGTTCGGG 
CGGCCCTGGC AGTCAGGCGA TGTCGTCGGC TGCATGATCG ACCTCACCGA GAACACCATC 
ATCTTCACCC TCAACGGCGA GGTCCTCATG TCTGACTCGG GCTCCGAAAC AGCCTTCCGG 
GAGATCGAGA TTGGGGACGG CTTCCTGCCT GTCTGCAGCT TGGGACCTGG CCAGGTGGGT 
CACCTGAACC TGGGCCAGGA TGTGAGCTCC CTGCGGTTCT TTGCCATCTG CGGCCTCCAG 
GAAGGCTTTG AGCCATTCGC CATCAACATG CAGCGCCCTG TCACCACGTG GTTCAGCAAA 
AGCCTTCCCC AGTTTGAGCC TGTGCCCCCT GAACACCCGC ACTATGAGGT GGCGCGCATG 
GATGGCACTG TGGACACGCC CCCCTGTCTG CGCCTGGCTC ACCGCACCTG GGGCTCCCAG 
AACAGCCTGG TGGAGATGCT GTTCCTCCGG CTGAGCCTTC CGGTCCAGTT CCATCAGCAC 
TTCCGTTGCA CCGCAGGGGC CACGCCCTTG GCACCTCCCG GCCTGCAACC CCCGGCGGAG 
GACGAGGCCC GGGCAGCAGA ACCCGACCCT GACTACGAAA ACCTGCGCCG CTCAGCTGGG 
GGCTGGGGCG AGGCTGAAGG GGGCAAAGAG GGAACTGCCA AGGAGGGGAC ACCCGGGGGC 
ACCCCCCAGC CTGGGGTGGA GGCCCAGCCC GTCAGGGCGG AGAACGAGAA GGATGCCACC 
ACGGAGAAGA ACAAGAAGAG AGGGTTCTTA TTCAAGGCCA AGAAGGCCGC CATGATGACT 
CAGCCACCAG CCACCCCTGC TCTGCCCCGG CTCCCTCACG ACGTGGTGCC TGCTGACAAC 
CGCGATGACC CCGAGATCAT CCTCAACACC ACCACGTACT ATTACTCCGT GAGGGTCTTC 
GCGGGTCAGG AGCCCAGCTG CGTATGGGTG GGCTGGGTCA CCCCCGACTA CCATCAGCAC 
GACATGAACT TTGACCTCAG CAAGGTCCGG GCAGTGACGG TGACCATGGG GGATGAACAA 
GGCAACGTCC ACAGCAGCCT CAAGTGCAGC AACTGCTACA TGGTGTGGGG TGGGGACTTC 
GTGAGCCCTG GGCAGCAGGG CCGGATCAGC CACACGGACC TTGTCATTGG CTGCCTGGTG 
GACCTGGCCA CTGGCTTAAT GACCTTCACG GCCAATGGCA AAGAGAGCAA CACCTTTTTC 
CAGGTGGAAC CCAACACAAA GCTCTTTCCT GCCGTGTTTG TCCTGCCCAC GCACCAGAAC 
GTCATCCAAT TTGAGCTGGG GAAGCAGAAG AACATCATGC CGCTGTCCGC CGCCATGTTC 
CTGAGCGAGC GCAAGAACCC AGCGCCACAG TGTCCACCAC GGCTGGAGGT GCAGATGCTG 
ATGCCCGTGT CCTGGAGCCG CATGCCCAAC CACTTCCTGC AGGTGGAGAC GCGGCGCGCT 
GGCGAGCGCC TTGGCTGGGC CGTGCAGTGC CAGGATCCCC TGACCATGAT GGCGCTGCAC 
ATTCCGGAGG AGAACCGGTG CATGGACATC CTGGAGCTGT CCGAGCGCCT GGACCTGCAG 
CGCTTCCACT CGCACACGCT GCGCCTCTAC CGCGCCGTGT GCGCCCTGGG CAACAACCGT 
GTGGCGCATG CTCTGTGCAG CCACGTGGAC CAGGCGCAGC TGCTGCACGC GCTGGAGGAC 
GCGCACCTGC CCGGCCCCCT GCGCGCGGGC TACTACGACC TCCTCATCAG CATCCACCTG 
GAAAGTGCCT GCCGCAGCCG GCGCTCCATG CTCTCCGAGT ACATCGTGCC GCTCACGCCC 
GAGACCCGCG CCATCACGCT CTTCCCGCCG GGAAGGAAGG GGGGAAACGC CCGCCGCCAC 
GGCCTGCCCG GCGTCGGCGT CACCACCTCG CTGCGGCCTC CGCACCATTT CTCCCCTCCG 
TGCTTCGTGG CCGCCCTGCC CGCCGCCGGG GTAGCGGAAG CCCCGGCCCG CCTCAGCCCT 
GCCATTCCTC TGGAGGCCCT GCGCGACAAG GCGCTGAGGA TGCTGGGAGA GGCCGTGCGC 
GATGGCGGGC AGCACGCCCG CGACCCCGTC GGGGGCTCCG TGGAGTTCCA GTTCGTGCCG 
GTGCTCAAGC TCGTGTCCAC CCTGCTGGTG ATGGGCATCT TTGGCGATGA GGATGTGAAG 
CAGATCTTAA AGATGATAGA ACCTGAAGTT TTCACCGAGG AAGAGGAGGA GGAAGAAGAG 
GAGGAGGAGG AAGAAGAGGA GGAAGAGGAG GACGAAGAGG AAAAGGAAGA GGACGAGGAG 
GAAGAGGAGA AGGAAGATGC TGAAAAAGAG GAAGAGGAGG CCCCGGAAGG GGAAAAAGAA 
GATTTGGAGG AGGGGCTGCT CCAGATGAAG TTGCCGGAGT CCGTGAAGCT ACAGATGTGC 
AACCTCCTGG AGTATTTCTG TGACCAAGAG CTACAGCATC GAGTGGAGTC CCTGGCGGCC 
TTCGCAGAGC GCTACGTGGA CAAGCTCCAG GCCAACCAGC GCAGCCGCTA CGCCCTCCTC 
ATGAGAGCCT TCACCATGAG TGCAGCTGAG ACCGCAAGGC GCACCCGCGA GTTCCGCTCC 
CCGCCCCAGG AGCAGATCAA CATGCTCTTG CACTTCAAAG ATGAGGCAGA TGAGGAAGAC 
TGCCCTCTCC CCGAGGACAT TCGACAGGAC TTGCAGGACT TTCATCAAGA CCTGCTGGCG 
CACTGTGGAA TCCAGCTGGA GGGGGAGGAG GAGGAGCCGG AGGAGGAGAC CAGCCTGAGC 
AGCCGCCTCC GGAGCCTGTT GGAGACGGTG CGGCTGGTGA AGAAGAAGGA GGAAAAACCC 
GAGGAGGAGC TGCCAGCCGA GGAGAAAAAA CCCCAGTCCC TGCAGGAGCT GGTGTCCCAC 
ATGGTGGTGC GCTGGGCCCA GGAGGACTAC GTGCAGAGCC CTGAGCTGGT GCGGGCCATG 
TTCAGCCTCC TGCACCGGCA GTACGATGGG CTCGGCGAGC TGCTGCGCGC CCTGCCCCGG 
GCCTACACCA TCTCGCCGTC CTCTGTGGAG GACACCATGA GCCTGCTCGA GTGCCTCGGC 
CAGATCCGCT CGCTGCTCAT CGTGCAGATG GGCCCCCAGG AGGAGAACCT CATGATCCAG 
AGCATTGGGA ACATCATGAA TAACAAAGTC TTCTACCAAC ACCCGAACCT GATGAGGGCC 
CTGGGCATGC ATGAGACCGT CATGGAGGTC ATGGTGAACG TCCTGGGGGG CGGCGAAACC 
AAGGAGATCC GCTTCCCCAA GATGGTGACA AGCTGCTGCC GCTTCCTCTG CTACTTCTGC 
CGCATCAGCC GGCAGAACCA GCGCTCCATG TTTGACCACC TGAGCTACCT GCTGGAGAAC 
AGCGGCATCG GCCTGGGTAT GCAAGGCTCC ACGCCCCTGG ATGTGGCTGC CGCCTCCGTT 
ATCGACAACA ACGAGCTGGC CTTGGCGTTG CAGGAGCAGG ACCTGGAAAA GGTCGTGTCC 
TACCTGGCAG GCTGTGGCCT CCAAAGCTGC CCCATGCTCC TGGCCAAGGG CTACCCGGAC 
ATCGGCTGGA ACCCCTGCGG TGGGGAGCGC TACCTGGACT TCCTGCGCTT CGCTGTCTTT 
GTTAACGGCG AGAGCGTGGA GGAGAACGCC AACGTGGTGG TGCGGCTGCT CATCCGCAAA 
CCTGAGTGCT TCGGGCCTGC GCTGCGGGGT GAGGGCGGCT CGGGGCTGCT GGCCGCCATC 
GAGGAGGCCA TCCGCATCTC TGAGGACCCC GCGCGGGACG GCCCAGGCGT CCGCAGGGAC 
CGGCGGCGCG AGCACTTTGG GGAGGAGCCC CCTGAAGAAA ACCGCGTGCA CCTGGGCCAC 
GCCATCATGT CCTTCTATGC TGCCTTGATC GACCTGCTGG GCCGCTGTGC TCCGGAGATG 
CATCTGATCC AAGCCGGCAA GGGTGAGGCC CTGCGAATCC GCGCCATCCT TCGCTCCCTC 
GTGCCCCTGG ACGACCTCGT GGGCATCATC AGCCTCCCGC TGCAGATCCC CACCCTGGGC 
AAAGACGGGG CCCTGGTGCA GCCGAAGATG TCGGCCTCCT TCGTGCCGGA CCACAAGGCA 
TCCATGGTGC TCTTCCTGGA CCGCGTGTAC GGCATTGAGA ACCAGGACTT CCTGCTGCAC 
GTGCTGGACG TGGGGTTCCT TCCCGACATG AGAGCTGCCG CCTCGCTGGA CACGGCCACT 
TTCAGCACCA CGGAGATGGC GCTGGCGCTG AACCGCTACC TGTGCCTGGC TGTGCTGCCG 
CTCATCACCA AGTGTGCGCC GCTCTTTGCG GGCACGGAGC ACCGTGCCAT CATGGTGGAC 
TCGATGCTGC ACACCGTGTA CCGCCTGTCG CGCGGCCGCT CGCTCACCAA GGCGCAGCGC 
GACGTCATCG AGGACTGTCT CATGGCGCTG TGCAGGTACA TCCGCCCGTC CATGCTGCAG 
CACCTGCTGC GGCGCCTGGT GTTCGACGTG CCCATCCTGA ACGAGTTCGC CAAGATGCCG 
CTCAAGCTCC TCACCAACCA CTACGAGCGC TGCTGGAAGT ACTACTGCCT CCCCACGGGC 
TGGGCCAACT TCGGGGTCAC CTCGGAGGAG GAGCTGCACC TCACACGCAA ACTCTTCTGG 
GGCATCTTCG ACTCTCTGGC CCACAAGAAG TACGACCAGG AGCTGTACCG CATGGCCATG 
CCTTGTCTGT GCGCCATCGC GGGGGCCCTG CCTCCCGACT ACGTGGACGC TTCGTACTCT 
TCCAAGGCGG AGAAAAAGGC CACAGTGGAT GCTGAAGGCA ACTTTGATCC CCGGCCTGTG 
GAGACCCTCA ATGTGATCAT CCCGGAGAAG CTCGACTCCT TCATCAACAA GTTTGCCGAA 
TACACGCACG AGAAGTGGGC CTTCGACAAG ATCCAGAACA ACTGGTCGTA CGGAGAGAAC 
GTGGATGAGG AACTGAAGAC CCACCCCATG CTGAGGCCCT ACAAGACCTT TTCGGAGAAG 
GACAAAGAGA TCTACCGCTG GCCCATCAAG GAGTCCCTGA AGGCCATGAT TGCCTGGGAG 
TGGACGATCG AGAAGGCCAG GGAAGGCGAG GAGGAGAGGA CGGAGAAGAA AAAGACACGG 
AAGATTTCCC AGACTGCCCA GACCTACGAT CCCCGAGAAG GCTACAACCC GCAGCCCCCC 
GACCTCAGCG GAGTCACCCT GTCTCGGGAG TTGCAGGCCA TGGCCGAACA ACTGGCGGAA 
AACTACCATA ACACCTGGGG GAGAAAGAAG AAGCAGGAGC TGGAAGCCAA GGGCGGCGGG 
ACCCACCCCC TGCTGGTCCC CTACGACACG CTCACGGCCA AGGAGAAAGC GCGAGACCGA 
GAGAAGGCCC AGGAGCTGCT GAAGTTCCTG CAGATGAACG GCTACGCGGT CACCAGAGGC 
CTGAAGGACA TGGAGCTGGA CACGTCTTCC ATTGAGAAGC GGTTCGCCTT TGGCTTCCTG 
CAGCAGTTGC TGCGCTGGAT GGACATTTCG CAGGAGTTCA TTGCCCACCT GGAGGCTGTG 
GTCAGCAGTG GACGAGTGGA GAAGTCCCCA CACGAACAGG AGATTAAATT CTTTGCCAAG 
ATCCTGCTCC CTTTGATTAA CCAGTACTTC ACCAACCACT GCCTCTACTT CCTGTCCACA 
CCCGCCAAAG TGCTGGGCAG TGGTGGCCAT GCCTCCAACA AGGAGAAGGA GATGATCACC 
AGCCTCTTCT GCAAACTCGC TGCCCTTGTC CGCCACCGAG TCTCTCTCTT CGGCACAGAC 
GCCCCGGCTG TGGTCAACTG CCTTCACATC CTGGCACGCT CCCTGGATGC CAGGACGGTG 
ATGAAGTCGG GCCCCGAGAT CGTCAAGGCC GGCCTCCGCT CCTTCTTTGA GAGCGCCTCG 
GAGGACATCG AGAAGATGGT GGAGAACTTG CGGCTGGGCA AGGTGTCCCA GGCGCGCACG 
CAGGTGAAGG GCGTGGGCCA GAACCTCACC TACACCACCG TGGCCCTGCT GCCCGTCCTC 
ACCACGCTCT TCCAGCACAT CGCCCAGCAC CAGTTCGGCG ACGACGTCAT CCTGGATGAC 
GTGCAGGTGT CTTGCTACCG AACATTGTGC AGCATCTACT CTCTGGGGAC CACCAAGAAC 
ACTTATGTGG AAAAGCTACG GCCAGCCCTT GGAGAGTGCC TGGCCCGCCT GGCAGCGGCC 
ATGCCTGTGG CCTTCCTGGA GCCTCAGCTG AACGAGTACA ACGCTTGCTC CGTGTACACC 
ACCAAGTCTC CCCGGGAGCG AGCCATCCTG GGGCTCCCCA ACAGCGTGGA GGAGATGTGT 
CCTGACATCC CGGTGCTGGA TCGGCTCATG GCAGACATCG GGGGGCTGGC CGAGTCCGGG 
GCCCGCTACA CGGAGATGCC GCACGTCATC GAGATCACGC TGCCCATGCT CTGCAGCTAC 
CTGCCCCGCT GGTGGGAGCG CGGGCCCGAG GCGCCCCCTC CCGCCCTGCC TGCCGGCGCC 
CCCCCGCCCT GCACGGCTGT CACCTCCGAC CACCTCAACT CACTCTTGGG GAACATCTTG 
CGCATCATTG TCAACAACCT GGGCATTGAC GAGGCCACCT GGATGAAGCG GCTTGCTGTG 
TTCGCCCAGC CCATCGTGAG CCGGGCGCGG CCTGAGCTCC TGCATTCCCA CTTCATCCCC 
ACCATCGGGC GGCTGCGCAA GCGGGCAGGC AAGGTGGTGG CCGAGGAGGA GCAGCTGCGG 
CTGGAGGCCA AGGCCGAGGC CGAGGAGGGC GAGCTCCTGG TGCGGGACGA GTTCTCTGTG 
CTCTGCCGGG ACCTGTACGC CCTCTACCCG CTGCTCATCC GCTACGTGGA CAACAACAGG 
GCGCACTGGC TGACGGAGCC CAACGCCAAC GCCGAGGAGC TGTTCAGGAT GGTGGGCGAG 
ATCTTCATCT ACTGGTCCAA GTCCCACAAC TTCAAGCGTG AGGAGCAGAA CTTTGTGGTC 
CAGAACGAGA TCAACAACAT GTCCTTCCTG ACGGCTGACA GCAAGAGCAA GATGGCCAAG 
GCGGGAGACG CACAGTCGGG CGGCTCGGAC CAGGAACGCA CCAAGAAGAA GCGCCGGGGG 
GACAGGTACT CCGTGCAGAC GTCACTGATC GTGGCCACGC TGAAGAAGAT GCTGCCCATT 
GGCCTGAACA TGTGTGCGCC CACCGACCAG GACCTCATCA TGCTGGCCAA GACCCGCTAC 
GCCCTGAAAG ACACAGACGA GGAGGTCCGG GAATTTCTGC AGAACAACCT CCACCTTCAG 
GGAAAGGTCG AAGGCTCCCC GTCCCTGCGC TGGCAGATGG CGCTGTACCG GGGCCTCCCC 
GGCCGCGAGG AGGACGCCGA CGACCCGGAG AAAATCGTGC GCAGGGTCCA AGAAGTGTCA 
GCGGTGCTCT ATCACCTGGA ACAGACCGAG CACCCCTACA AGTCCAAGAA GGCCGTGTGG 
CACAAGCTGT TGTCCAAGCA GCGCCGGCGA GCCGTGGTGG CCTGCTTCCG TATGACGCCC 
CTGTACAACC TGCCCACGCA CCGGGCCTGT AACATGTTCT TGGAGAGCTA CAAAGCCGCC 
TGGATCCTGA CCGAAGATCA CAGTTTCGAG GACCGCATGA TAGATGATCT TTCAAAAGCT 
GGGGAGCAGG AGGAGGAGGA GGAGGAAGTG GAGGAGAAGA AGCCAGACCC CCTGCACCAG 
CTGGTCCTGC ACTTCAGCCG CACGGCCCTG ACTGAAAAGA GCAAACTGGA CGAGGACTAC 
CTGTACATGG CATATGCCGA CATCATGGCC AAGAGCTGCC ACCTGGAGGA GGGAGGGGAG 
AACGGTGAAG CTGAGGAAGA GGAGGTTGAG GTCTCCTTTG AGGAGAAAGA GATGGAGAAG 
CAGAGGCTCC TCTACCAGCA GTCACGGCTG CACACGCGCG GGGCCGCCGA GATGGTGCTG 
CAGATGATCA GCGCCTGCAA AGGAGAGACA GGTGCCATGG TGTCCTCCAC CCTGAAGCTG 
GGCATCTCCA TCCTCAATGG AGGCAATGCC GAGGTGCAGC AGAAAATGCT GGATTATCTG 
AAGGACAAGA AGGAAGTCGG CTTCTTCCAG AGCATCCAGG CGCTGATGCA GACATGCAGC 
GTCCTGGATC TCAATGCCTT CGAGAGACAG AACAAGGCAG AAGGCCTGGG CATGGTGAAC 
GAGGATGGAA CCGTCATCAA TCGCCAGAAC GGAGAGAAGG TTATGGCCGA TGACGAATTC 
ACACAAGATC TGTTCCGATT TCTGCAGCTG CTCTGTGAGG GGCACAACAA CGATTTCCAA 
AACTACTTAC GGACACAGAC GGGCAACACC ACCACGATTA ACATCATTAT CTGCACAGTG 
GACTACCTCC TGCGGCTGCA GGAGTCCATC AGCGACTTCT ACTGGTACTA CTCCGGCAAG 
GATGTCATTG AGGAGCAGGG CAAGAGGAAC TTCTCCAAGG CCATGTCCGT GGCCAAACAG 
GTGTTCAACA GCCTCACCGA GTACATCCAG GGCCCCTGCA CCGGGAACCA GCAGAGCCTG 
GCGCACAGCC GCCTCTGGGA CGCCGTGGTG GGATTCCTGC ACGTGTTTGC CCACATGATG 
ATGAAGCTCG CTCAGGACTC GAGCCAGATC GAGCTGTTGA AGGAGCTGCT GGACCTGCAG 
AAGGACATGG TGGTGATGCT GCTGTCCCTA CTGGAAGGGA ACGTGGTGAA CGGCATGATT 
GCCCGGCAGA TGGTGGACAT GCTGGTGGAG TCCTCGTCCA ACGTGGAGAT GATCCTCAAG 
TTCTTCGACA TGTTCCTGAA ACTCAAGGAC ATCGTGGGCT CCGAGGCCTT CCAGGACTAC 
GTCACCGATC CCCGTGGCCT CATCTCCAAA AAGGACTTCC AGAAGGCCAT GGACAGCCAG 
AAGCAGTTCA CTGGGCCGGA AATCCAGTTT CTGCTCTCGT GCTCCGAAGC CGACGAGAAT 
GAGATGATCA ACTTCGAGGA GTTCGCCAAC CGCTTCCAGG AGCCAGCCCG GGACATCGGC 
TTCAACGTGG CCGTGCTGCT GACCAACCTG TCGGAGCACG TGCCGCACGA CCCGCGCCTG 
CGCAACTTCC TGGAGCTGGC TGAGAGCATC CTTGAGTACT TCCGGCCCTA CCTGGGCCGC 
ATCGAGATCA TGGGCGCCTC CCGCCGCATC GAGCGCATCT ACTTCGAGAT CTCAGAGACC 
AACCGCGCCC AGTGGGAGAT GCCCCAGGTC AAGGAGTCCA AGCGCCAGTT CATCTTCGAC 
GTGGTCAACG AGGGCGGGGA GGCCGAGAAG ATGGAGCTGT TCGTGAGCTT CTGCGAGGAC 
ACCATCTTCG AGATGCAGAT CGCCGCGCAG ATCTCCGAGC CCGAGGGCGA GCCCGAGGCC 
GACGAGGACG AGGGCATGGG CGAGGCGGCC GCCGAGGGCG CCGAGGAGGG CGCGGCGGGC 
GCCGAGGGCG CGGCGGGCAC GGTGGCGGCG GGCGCCACGG CGCGGCTGGC GGCGGCCGCG 
GCCCGGGCGC TGCGCGGCCT CAGCTACCGC AGCCTGCGGC GGCGCGTGCG GCGGCTGCGG 
CGGCTCACGG CGCGCGAGGC GGCCACGGCG CTGGCCGCGC TGCTCTGGGC CGTGGTGGCG 
CGCGCGGGCG CGGCGGGCGC GGGCGCGGCG GCGGGCGCGC TGCGCCTGCT CTGGGGCTCG 
CTCTTCGGCG GCGGCCTGGT GGAGGGCGCC AAGAAGGTGA CGGTGACCGA GCTGCTGGCC 
GGCATGCCCG ATCCCACCAG CGACGAGGTG CACGGCGAGC AGCCGGCCGG GCCCGGCGGC 
GACGCGGACG GCGCGGGCGA GGGCGAAGGC GAGGGCGACG CGGCCGAGGG TGACGGCGAT 
GAGGAGGTGG CGGGTCACGA GGCCGGCCCG GGCGGTGCCG AGGGGGTCGT GGCCGTGGCC 
GACGGGGGCC CCTTCCGGCC CGAGGGCGCT GGCGGCCTTG GGGACATGGG CGACACCACG 
CCGGCGGAAC CGCCCACGCC CGAGGGCTCC CCGATCCTCA AGAGGAAGCT GGGGGTGGAT 
GGAGAAGAGG AGGAGCTGGT TCCAGAACCG GAGCCCGAGC CCGAGCCTGA GCCTGAGAAA 
GCCGATGAGG AGAACGGGGA GAAGGAGGAA GTCCCCGAGG CCCCACCAGA ACCCCCCAAG 
AAAGCCCCTC CCTCACCTCC TGCAAAGAAG GAGGAAGCCG GAGGCGCCGG CATGGAGTTC 
TGGGGAGAAC TGGAAGTGCA GAGGGTGAAG TTCTTGAACT ACCTCTCCCG GAACTTCTAC 
ACCCTGCGCT TCCTTGCCCT CTTCTTGGCG TTTGCCATCA ACTTCATCCT GCTGTTTTAT 
AAGGTCTCAG ACTCTCCACC AGGGGAGGAT GACATGGAGG GCTCGGCAGC AGGGGACCTG 
GCGGGTGCAG GGTCCGGGGG CGGCTCCGGC TGGGGCTCGG GGGCCGGCGA GGAGGCAGAG 
GGGGACGAGG ACGAGAACAT GGTATACTAC TTCCTGGAGG AGAGCACGGG CTACATGGAG 
CCGGCCCTGT GGTGCCTGAG CCTGCTGCAC ACGCTGGTGG CCTTCCTCTG CATCATCGGC 
TACAACTGCC TCAAGGTGCC CCTGGTGATC TTCAAGCGGG AGAAGGAGCT GGCACGAAAG 
CTGGAGTTTG ACGGCCTGTA CATCACAGAG CAGCCTGGGG ACGACGACGT GAAGGGCCAG 
TGGGACCGGC TGGTGCTCAA CACGCCGTCT TTCCCCAGCA ACTACTGGGA CAAGTTTGTC 
AAGCGGAAGG TCCTGGACAA ACACGGGGAC ATCTTCGGGC GGGAGCGGAT TGCCGAGCTG 
CTGGGCATGG ACCTGGCCTC GCTGGAGATC ACGGCCCACA ACGAGCGCAA GCCCGACCCC 
CCGCCGGGGC TGCTGACCTG GCTCATGTCC ATCGATGTCA AGTACCAGAT CTGGAAGTTC 
GGGGTCATCT TCACGGACAA CTCGTTCCTG TACCTGGGCT GGTACATGGT GATGTCCCTC 
CTGGGCCACT ACAACAACTT CTTCTTTGCC GCCCACCTGC TGGACATCGC CATGGGGGTC 
AAGACGCTGC GTACCATCCT CTCCTCTGTC ACCCACAATG GGAAACAGCT GGTGATGACT 
GTGGGCCTCC TGGCCGTCGT GGTCTACCTG TACACTGTGG TGGCCTTCAA CTTCTTCCGC 
AAGTTCTACA ACAAGAGCGA GGACGAGGAC GAGCCGGACA TGAAGTGCGA TGACATGATG 
ACGTGCTACC TGTTCCACAT GTACGTGGGC GTCCGGGCTG GCGGAGGCAT CGGGGACGAG 
ATCGAGGACC CAGCGGGCGA TGAATACGAG CTCTACCGGG TGGTCTTCGA CATCACCTTC 
TTCTTCTTCG TCATTGTCAT CCTGCTGGCC ATCATCCAGG GTCTGATTAT CGACGCCTTC 
GGCGAGCTCC GAGACCAGCA GGAGCAAGTG AAGGAAGATA TGGAGACCAA ATGCTTCATC 
TGCGGGATTG GCAGTGACTA CTTCGATACC ACGCCGCACG GCTTCGAGAC CCACACGCTA 
GAGGAGCACA ATCTGGCCAA TTACATGTTC TTCTTGATGT ATCTGATAAA CAAGGACGAG 
ACGGAGCACA CGGGCCAGGA GTCCTACGTC TGGAAGATGT ATCAGGAGAG GTGCTGGGAC 
TTCTTCCCCG CCGGCGACTG CTTCCGCAAG CAGTACGAGG ACCAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095188.1
CDS	594..15707
Translation	MGDGGEGEDEVQFLRTDDEVVLQCSATVLKEQLKLCLAAEGFGNRLCFLEPTSNAQNVPPDLAICCFTLEQSLSVRALQEMLANTVEAGVESSQGGGHRTLLYGHAILLRHAHSRMYLSCLTTSRSMTDKLAFDVGLQEDATGEACWWTMHPASKQRSEGEKVRVGDDLILVSVSSERYLHLSTASGELQVDASFMQTLWNMNPICSCCEEGYVTGGHVLRLFHGHMDECLTISAADSDDQRRLVYYEGGAVCTHARSLWRLEPLRISWSGSHLRWGQPLRIRHVTTGRYLALTEDQGLVVVDACKAHTKATSFCFRVSKEKLDTAPKRDVEGMGPPEIKYGESLCFVQHVASGLWLTYAAPDPKALRLGVLKKKAILHQEGHMDDALFLTRCQQEESQAARMIHSTAGLYNQFIKGLDSFSGKPRGSGPPAGPALPIEAVILSLQDLIGYFEPPSEELQHEEKQSKLRSLRNRQSLFQEEGMLSLVLNCIDRLNVYTTAAHFAEYAGEEAAESWKEIVNLLYELLASLIRGNRANCALFSTNLDWVVSKLDRLEASSGILEVLYCVLIESPEVLNIIQENHIKSIISLLDKHGRNHKVLDVLCSLCVCNGVAVRSNQDLITENLLPGRELLLQTNLINYVTSIRPNIFVGRAEGSTQYGKWYFEVMVDEVVPFLTAQATHLRVGWALTEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWTGHVARPVTSPGQHLLAPEDVVSCCLDLSVPSISFRINGCPVQGVFEAFNLDGLFFPVVSFSAGVKVRFLLGGRHGEFKFLPPPGYAPCHEAVLPRERLRLEPIKEYRREGPRGPHLVGPSRCLSHTDFVPCPVDTVQIVLPPHLERIREKLAENIHELWALTRIEQGWTYGPVRDDNKRLHPCLVNFHSLPEPERNYNLQMSGETLKTLLALGCHVGMADEKAEDNLKKTKLPKTYMMSNGYKPAPLDLSHVRLTPAQTTLVDRLAENGHNVWARDRVAQGWSYSAVQDIPARRNPRLVPYRLLDEATKRSNRDSLCQAVRTLLGYGYNIEPPDQEPSQVENQSRWDRVRIFRAEKSYTVQSGRWYFEFEAVTTGEMRVGWARPELRPDVELGADELAYVFNGHRGQRWHLGSEPFGRPWQSGDVVGCMIDLTENTIIFTLNGEVLMSDSGSETAFREIEIGDGFLPVCSLGPGQVGHLNLGQDVSSLRFFAICGLQEGFEPFAINMQRPVTTWFSKSLPQFEPVPPEHPHYEVARMDGTVDTPPCLRLAHRTWGSQNSLVEMLFLRLSLPVQFHQHFRCTAGATPLAPPGLQPPAEDEARAAEPDPDYENLRRSAGGWGEAEGGKEGTAKEGTPGGTPQPGVEAQPVRAENEKDATTEKNKKRGFLFKAKKAAMMTQPPATPALPRLPHDVVPADNRDDPEIILNTTTYYYSVRVFAGQEPSCVWVGWVTPDYHQHDMNFDLSKVRAVTVTMGDEQGNVHSSLKCSNCYMVWGGDFVSPGQQGRISHTDLVIGCLVDLATGLMTFTANGKESNTFFQVEPNTKLFPAVFVLPTHQNVIQFELGKQKNIMPLSAAMFLSERKNPAPQCPPRLEVQMLMPVSWSRMPNHFLQVETRRAGERLGWAVQCQDPLTMMALHIPEENRCMDILELSERLDLQRFHSHTLRLYRAVCALGNNRVAHALCSHVDQAQLLHALEDAHLPGPLRAGYYDLLISIHLESACRSRRSMLSEYIVPLTPETRAITLFPPGRKGGNARRHGLPGVGVTTSLRPPHHFSPPCFVAALPAAGVAEAPARLSPAIPLEALRDKALRMLGEAVRDGGQHARDPVGGSVEFQFVPVLKLVSTLLVMGIFGDEDVKQILKMIEPEVFTEEEEEEEEEEEEEEEEEEDEEEKEEDEEEEEKEDAEKEEEEAPEGEKEDLEEGLLQMKLPESVKLQMCNLLEYFCDQELQHRVESLAAFAERYVDKLQANQRSRYALLMRAFTMSAAETARRTREFRSPPQEQINMLLHFKDEADEEDCPLPEDIRQDLQDFHQDLLAHCGIQLEGEEEEPEEETSLSSRLRSLLETVRLVKKKEEKPEEELPAEEKKPQSLQELVSHMVVRWAQEDYVQSPELVRAMFSLLHRQYDGLGELLRALPRAYTISPSSVEDTMSLLECLGQIRSLLIVQMGPQEENLMIQSIGNIMNNKVFYQHPNLMRALGMHETVMEVMVNVLGGGETKEIRFPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLDVAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWNPCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRKPECFGPALRGEGGSGLLAAIEEAIRISEDPARDGPGVRRDRRREHFGEEPPEENRVHLGHAIMSFYAALIDLLGRCAPEMHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALVQPKMSASFVPDHKASMVLFLDRVYGIENQDFLLHVLDVGFLPDMRAAASLDTATFSTTEMALALNRYLCLAVLPLITKCAPLFAGTEHRAIMVDSMLHTVYRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEFAKMPLKLLTNHYERCWKYYCLPTGWANFGVTSEEELHLTRKLFWGIFDSLAHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKKATVDAEGNFDPRPVETLNVIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSYGENVDEELKTHPMLRPYKTFSEKDKEIYRWPIKESLKAMIAWEWTIEKAREGEEERTEKKKTRKISQTAQTYDPREGYNPQPPDLSGVTLSRELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKARDREKAQELLKFLQMNGYAVTRGLKDMELDTSSIEKRFAFGFLQQLLRWMDISQEFIAHLEAVVSSGRVEKSPHEQEIKFFAKILLPLINQYFTNHCLYFLSTPAKVLGSGGHASNKEKEMITSLFCKLAALVRHRVSLFGTDAPAVVNCLHILARSLDARTVMKSGPEIVKAGLRSFFESASEDIEKMVENLRLGKVSQARTQVKGVGQNLTYTTVALLPVLTTLFQHIAQHQFGDDVILDDVQVSCYRTLCSIYSLGTTKNTYVEKLRPALGECLARLAAAMPVAFLEPQLNEYNACSVYTTKSPRERAILGLPNSVEEMCPDIPVLDRLMADIGGLAESGARYTEMPHVIEITLPMLCSYLPRWWERGPEAPPPALPAGAPPPCTAVTSDHLNSLLGNILRIIVNNLGIDEATWMKRLAVFAQPIVSRARPELLHSHFIPTIGRLRKRAGKVVAEEEQLRLEAKAEAEEGELLVRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNANAEELFRMVGEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAKAGDAQSGGSDQERTKKKRRGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIMLAKTRYALKDTDEEVREFLQNNLHLQGKVEGSPSLRWQMALYRGLPGREEDADDPEKIVRRVQEVSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPTHRACNMFLESYKAAWILTEDHSFEDRMIDDLSKAGEQEEEEEEVEEKKPDPLHQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEEGGENGEAEEEEVEVSFEEKEMEKQRLLYQQSRLHTRGAAEMVLQMISACKGETGAMVSSTLKLGISILNGGNAEVQQKMLDYLKDKKEVGFFQSIQALMQTCSVLDLNAFERQNKAEGLGMVNEDGTVINRQNGEKVMADDEFTQDLFRFLQLLCEGHNNDFQNYLRTQTGNTTTINIIICTVDYLLRLQESISDFYWYYSGKDVIEEQGKRNFSKAMSVAKQVFNSLTEYIQGPCTGNQQSLAHSRLWDAVVGFLHVFAHMMMKLAQDSSQIELLKELLDLQKDMVVMLLSLLEGNVVNGMIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQFLLSCSEADENEMINFEEFANRFQEPARDIGFNVAVLLTNLSEHVPHDPRLRNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNRAQWEMPQVKESKRQFIFDVVNEGGEAEKMELFVSFCEDTIFEMQIAAQISEPEGEPEADEDEGMGEAAAEGAEEGAAGAEGAAGTVAAGATARLAAAAARALRGLSYRSLRRRVRRLRRLTAREAATALAALLWAVVARAGAAGAGAAAGALRLLWGSLFGGGLVEGAKKVTVTELLAGMPDPTSDEVHGEQPAGPGGDADGAGEGEGEGDAAEGDGDEEVAGHEAGPGGAEGVVAVADGGPFRPEGAGGLGDMGDTTPAEPPTPEGSPILKRKLGVDGEEEELVPEPEPEPEPEPEKADEENGEKEEVPEAPPEPPKKAPPSPPAKKEEAGGAGMEFWGELEVQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPGEDDMEGSAAGDLAGAGSGGGSGWGSGAGEEAEGDEDENMVYYFLEESTGYMEPALWCLSLLHTLVAFLCIIGYNCLKVPLVIFKREKELARKLEFDGLYITEQPGDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLDKHGDIFGRERIAELLGMDLASLEITAHNERKPDPPPGLLTWLMSIDVKYQIWKFGVIFTDNSFLYLGWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHNGKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLS

Target ORF information:

RefSeq Version	NM_001101718.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus ryanodine receptor 1 (RYR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101718.1

ORF Insert Sequence:

ATGGGTGACG GAGGAGAGGG CGAGGACGAA GTCCAGTTCC TGCGGACGGA CGACGAGGTG 
GTCCTGCAGT GCAGCGCCAC CGTCCTCAAG GAGCAGCTGA AGCTGTGCCT GGCCGCCGAG 
GGCTTCGGCA ACCGCCTCTG CTTCCTGGAG CCCACCAGCA ACGCCCAGAA TGTGCCCCCC 
GACCTGGCCA TCTGCTGCTT CACCCTGGAG CAGTCCCTGT CGGTCCGCGC CCTGCAGGAG 
ATGCTGGCCA ACACCGTGGA GGCTGGCGTG GAGTCATCCC AGGGCGGGGG ACACAGGACA 
CTCCTGTATG GCCATGCCAT CCTGCTCCGG CATGCACACA GCCGCATGTA CCTGAGCTGC 
CTCACTACCT CGCGCTCCAT GACTGACAAG CTGGCTTTTG ACGTGGGACT GCAGGAGGAT 
GCAACAGGAG AGGCCTGTTG GTGGACCATG CACCCAGCCT CCAAGCAGAG ATCGGAAGGA 
GAGAAGGTCC GCGTCGGGGA CGACCTCATC CTTGTCAGCG TCTCCTCCGA GCGCTACCTG 
CACCTGTCGA CGGCCAGTGG GGAGCTCCAG GTTGATGCCT CCTTCATGCA GACCCTGTGG 
AACATGAACC CCATCTGCTC CTGCTGTGAA GAGGGCTACG TGACTGGGGG CCACGTCCTC 
CGCCTCTTCC ACGGACACAT GGACGAGTGC TTGACTATTT CTGCTGCTGA CAGCGACGAC 
CAGCGCAGGC TTGTCTACTA CGAGGGGGGA GCTGTGTGCA CGCACGCCCG CTCCCTCTGG 
AGGCTGGAAC CCCTGAGAAT CAGCTGGAGT GGAAGCCACC TGCGCTGGGG CCAGCCACTG 
CGGATCCGGC ATGTCACCAC CGGACGGTAC CTGGCACTCA CCGAGGACCA GGGCCTGGTG 
GTGGTCGATG CCTGCAAGGC CCACACCAAG GCCACCTCCT TCTGTTTCCG TGTCTCCAAG 
GAGAAGCTGG ACACGGCCCC CAAGCGAGAC GTGGAGGGCA TGGGCCCGCC TGAGATCAAG 
TACGGGGAGT CGCTGTGCTT TGTGCAGCAC GTGGCCTCGG GCCTGTGGCT CACCTACGCC 
GCCCCTGACC CCAAGGCCCT GCGGCTGGGC GTGCTGAAGA AGAAGGCCAT TCTGCACCAG 
GAGGGCCACA TGGACGATGC ACTGTTCCTG ACCCGCTGCC AGCAGGAGGA GTCCCAGGCT 
GCCCGCATGA TCCACAGCAC TGCCGGCCTG TACAACCAGT TCATCAAGGG CCTGGACAGC 
TTCAGCGGGA AGCCCCGGGG CTCAGGGCCG CCGGCTGGCC CTGCGCTGCC CATCGAGGCC 
GTCATCCTGA GCCTGCAGGA CCTCATTGGC TACTTCGAGC CGCCCTCGGA GGAGCTGCAG 
CACGAGGAAA AGCAGAGCAA GCTGCGCAGC CTGCGCAACC GCCAGAGCCT CTTCCAGGAG 
GAGGGGATGC TCTCCCTGGT CCTGAACTGC ATCGACCGCC TGAACGTCTA CACCACCGCT 
GCCCACTTTG CCGAGTATGC AGGGGAAGAG GCAGCTGAGT CCTGGAAAGA GATTGTTAAC 
CTACTCTACG AACTCCTGGC CTCTCTGATC CGGGGCAATC GTGCCAACTG TGCCCTCTTC 
TCCACAAACT TGGACTGGGT GGTCAGCAAA CTGGACCGGC TGGAGGCCTC GTCTGGCATC 
CTGGAGGTGC TGTACTGTGT CCTGATTGAG AGTCCCGAGG TGCTGAACAT CATCCAGGAG 
AACCACATCA AGTCCATCAT CTCCCTCCTG GACAAGCATG GGAGGAACCA CAAGGTTCTG 
GATGTGCTGT GCTCCCTGTG TGTGTGCAAT GGCGTGGCTG TGCGCTCCAA CCAAGATCTC 
ATCACCGAGA ATCTGCTCCC TGGCCGTGAG CTGCTGCTGC AGACAAACCT TATCAACTAC 
GTCACCAGCA TCCGCCCCAA CATCTTCGTG GGCCGAGCCG AGGGCTCCAC GCAGTATGGC 
AAATGGTACT TTGAGGTGAT GGTGGACGAG GTGGTTCCAT TTCTGACAGC TCAGGCCACC 
CATCTGCGGG TGGGCTGGGC CCTCACCGAG GGCTACAGCC CCTACCCTGG CGGCGGCGAG 
GGCTGGGGCG GCAATGGGGT CGGCGATGAC CTCTACTCCT ATGGCTTTGA CGGGCTGCAC 
CTCTGGACAG GACACGTGGC ACGCCCGGTG ACCTCCCCCG GGCAGCACCT CCTGGCCCCT 
GAGGACGTGG TCAGCTGCTG CCTGGACCTC AGCGTGCCGT CCATCTCCTT CCGCATCAAT 
GGCTGCCCCG TGCAGGGTGT CTTTGAGGCC TTCAACCTGG ATGGGCTCTT CTTCCCTGTG 
GTCAGCTTCT CGGCTGGTGT CAAGGTGCGG TTTCTCCTTG GCGGCCGCCA TGGTGAATTC 
AAGTTCCTGC CCCCACCCGG CTACGCCCCA TGCCATGAGG CAGTGCTCCC GCGAGAGCGA 
CTCCGTCTTG AGCCCATCAA GGAGTACAGA CGGGAAGGGC CGCGGGGGCC CCACCTGGTG 
GGCCCCAGCC GCTGCCTCTC GCACACTGAC TTTGTGCCCT GCCCGGTGGA CACCGTCCAG 
ATCGTCCTGC CTCCCCACCT GGAGCGCATC CGTGAGAAGC TGGCAGAGAA CATCCATGAA 
CTCTGGGCGC TGACGCGCAT TGAGCAAGGC TGGACCTATG GCCCGGTTCG GGATGACAAC 
AAGAGGCTGC ACCCGTGTCT TGTGAACTTC CACAGCCTTC CGGAGCCCGA GAGGAATTAC 
AACCTGCAGA TGTCGGGCGA GACTCTCAAG ACTCTGCTGG CGCTGGGCTG CCACGTGGGC 
ATGGCCGACG AGAAGGCGGA GGACAACCTG AAGAAGACAA AGCTGCCCAA GACGTATATG 
ATGAGCAATG GGTACAAGCC AGCCCCGCTG GACTTGAGCC ATGTGCGACT GACACCCGCA 
CAGACGACAC TGGTGGACCG GCTGGCAGAG AATGGGCACA ACGTGTGGGC ACGAGACCGA 
GTGGCCCAGG GCTGGAGCTA CAGCGCCGTG CAGGACATCC CGGCTCGCCG GAACCCTCGC 
CTTGTGCCCT ACCGCCTGCT GGACGAGGCC ACCAAGCGCA GCAACCGGGA CAGCCTCTGC 
CAGGCCGTGC GCACCCTGCT GGGCTATGGC TACAACATCG AGCCACCTGA CCAGGAGCCC 
AGTCAGGTGG AGAACCAGTC TCGCTGGGAC CGGGTGCGCA TCTTCCGGGC AGAGAAATCC 
TACACAGTGC AGAGTGGCCG CTGGTACTTC GAGTTTGAGG CCGTCACCAC AGGCGAGATG 
CGAGTGGGCT GGGCGAGGCC CGAGCTGCGG CCTGACGTCG AGCTGGGAGC GGATGAGCTG 
GCCTACGTCT TCAACGGGCA CCGTGGTCAG CGCTGGCACT TGGGCAGTGA GCCGTTCGGG 
CGGCCCTGGC AGTCAGGCGA TGTCGTCGGC TGCATGATCG ACCTCACCGA GAACACCATC 
ATCTTCACCC TCAACGGCGA GGTCCTCATG TCTGACTCGG GCTCCGAAAC AGCCTTCCGG 
GAGATCGAGA TTGGGGACGG CTTCCTGCCT GTCTGCAGCT TGGGACCTGG CCAGGTGGGT 
CACCTGAACC TGGGCCAGGA TGTGAGCTCC CTGCGGTTCT TTGCCATCTG CGGCCTCCAG 
GAAGGCTTTG AGCCATTCGC CATCAACATG CAGCGCCCTG TCACCACGTG GTTCAGCAAA 
AGCCTTCCCC AGTTTGAGCC TGTGCCCCCT GAACACCCGC ACTATGAGGT GGCGCGCATG 
GATGGCACTG TGGACACGCC CCCCTGTCTG CGCCTGGCTC ACCGCACCTG GGGCTCCCAG 
AACAGCCTGG TGGAGATGCT GTTCCTCCGG CTGAGCCTTC CGGTCCAGTT CCATCAGCAC 
TTCCGTTGCA CCGCAGGGGC CACGCCCTTG GCACCTCCCG GCCTGCAACC CCCGGCGGAG 
GACGAGGCCC GGGCAGCAGA ACCCGACCCT GACTACGAAA ACCTGCGCCG CTCAGCTGGG 
GGCTGGGGCG AGGCTGAAGG GGGCAAAGAG GGAACTGCCA AGGAGGGGAC ACCCGGGGGC 
ACCCCCCAGC CTGGGGTGGA GGCCCAGCCC GTCAGGGCGG AGAACGAGAA GGATGCCACC 
ACGGAGAAGA ACAAGAAGAG AGGGTTCTTA TTCAAGGCCA AGAAGGCCGC CATGATGACT 
CAGCCACCAG CCACCCCTGC TCTGCCCCGG CTCCCTCACG ACGTGGTGCC TGCTGACAAC 
CGCGATGACC CCGAGATCAT CCTCAACACC ACCACGTACT ATTACTCCGT GAGGGTCTTC 
GCGGGTCAGG AGCCCAGCTG CGTATGGGTG GGCTGGGTCA CCCCCGACTA CCATCAGCAC 
GACATGAACT TTGACCTCAG CAAGGTCCGG GCAGTGACGG TGACCATGGG GGATGAACAA 
GGCAACGTCC ACAGCAGCCT CAAGTGCAGC AACTGCTACA TGGTGTGGGG TGGGGACTTC 
GTGAGCCCTG GGCAGCAGGG CCGGATCAGC CACACGGACC TTGTCATTGG CTGCCTGGTG 
GACCTGGCCA CTGGCTTAAT GACCTTCACG GCCAATGGCA AAGAGAGCAA CACCTTTTTC 
CAGGTGGAAC CCAACACAAA GCTCTTTCCT GCCGTGTTTG TCCTGCCCAC GCACCAGAAC 
GTCATCCAAT TTGAGCTGGG GAAGCAGAAG AACATCATGC CGCTGTCCGC CGCCATGTTC 
CTGAGCGAGC GCAAGAACCC AGCGCCACAG TGTCCACCAC GGCTGGAGGT GCAGATGCTG 
ATGCCCGTGT CCTGGAGCCG CATGCCCAAC CACTTCCTGC AGGTGGAGAC GCGGCGCGCT 
GGCGAGCGCC TTGGCTGGGC CGTGCAGTGC CAGGATCCCC TGACCATGAT GGCGCTGCAC 
ATTCCGGAGG AGAACCGGTG CATGGACATC CTGGAGCTGT CCGAGCGCCT GGACCTGCAG 
CGCTTCCACT CGCACACGCT GCGCCTCTAC CGCGCCGTGT GCGCCCTGGG CAACAACCGT 
GTGGCGCATG CTCTGTGCAG CCACGTGGAC CAGGCGCAGC TGCTGCACGC GCTGGAGGAC 
GCGCACCTGC CCGGCCCCCT GCGCGCGGGC TACTACGACC TCCTCATCAG CATCCACCTG 
GAAAGTGCCT GCCGCAGCCG GCGCTCCATG CTCTCCGAGT ACATCGTGCC GCTCACGCCC 
GAGACCCGCG CCATCACGCT CTTCCCGCCG GGAAGGAAGG GGGGAAACGC CCGCCGCCAC 
GGCCTGCCCG GCGTCGGCGT CACCACCTCG CTGCGGCCTC CGCACCATTT CTCCCCTCCG 
TGCTTCGTGG CCGCCCTGCC CGCCGCCGGG GTAGCGGAAG CCCCGGCCCG CCTCAGCCCT 
GCCATTCCTC TGGAGGCCCT GCGCGACAAG GCGCTGAGGA TGCTGGGAGA GGCCGTGCGC 
GATGGCGGGC AGCACGCCCG CGACCCCGTC GGGGGCTCCG TGGAGTTCCA GTTCGTGCCG 
GTGCTCAAGC TCGTGTCCAC CCTGCTGGTG ATGGGCATCT TTGGCGATGA GGATGTGAAG 
CAGATCTTAA AGATGATAGA ACCTGAAGTT TTCACCGAGG AAGAGGAGGA GGAAGAAGAG 
GAGGAGGAGG AAGAAGAGGA GGAAGAGGAG GACGAAGAGG AAAAGGAAGA GGACGAGGAG 
GAAGAGGAGA AGGAAGATGC TGAAAAAGAG GAAGAGGAGG CCCCGGAAGG GGAAAAAGAA 
GATTTGGAGG AGGGGCTGCT CCAGATGAAG TTGCCGGAGT CCGTGAAGCT ACAGATGTGC 
AACCTCCTGG AGTATTTCTG TGACCAAGAG CTACAGCATC GAGTGGAGTC CCTGGCGGCC 
TTCGCAGAGC GCTACGTGGA CAAGCTCCAG GCCAACCAGC GCAGCCGCTA CGCCCTCCTC 
ATGAGAGCCT TCACCATGAG TGCAGCTGAG ACCGCAAGGC GCACCCGCGA GTTCCGCTCC 
CCGCCCCAGG AGCAGATCAA CATGCTCTTG CACTTCAAAG ATGAGGCAGA TGAGGAAGAC 
TGCCCTCTCC CCGAGGACAT TCGACAGGAC TTGCAGGACT TTCATCAAGA CCTGCTGGCG 
CACTGTGGAA TCCAGCTGGA GGGGGAGGAG GAGGAGCCGG AGGAGGAGAC CAGCCTGAGC 
AGCCGCCTCC GGAGCCTGTT GGAGACGGTG CGGCTGGTGA AGAAGAAGGA GGAAAAACCC 
GAGGAGGAGC TGCCAGCCGA GGAGAAAAAA CCCCAGTCCC TGCAGGAGCT GGTGTCCCAC 
ATGGTGGTGC GCTGGGCCCA GGAGGACTAC GTGCAGAGCC CTGAGCTGGT GCGGGCCATG 
TTCAGCCTCC TGCACCGGCA GTACGATGGG CTCGGCGAGC TGCTGCGCGC CCTGCCCCGG 
GCCTACACCA TCTCGCCGTC CTCTGTGGAG GACACCATGA GCCTGCTCGA GTGCCTCGGC 
CAGATCCGCT CGCTGCTCAT CGTGCAGATG GGCCCCCAGG AGGAGAACCT CATGATCCAG 
AGCATTGGGA ACATCATGAA TAACAAAGTC TTCTACCAAC ACCCGAACCT GATGAGGGCC 
CTGGGCATGC ATGAGACCGT CATGGAGGTC ATGGTGAACG TCCTGGGGGG CGGCGAAACC 
AAGGAGATCC GCTTCCCCAA GATGGTGACA AGCTGCTGCC GCTTCCTCTG CTACTTCTGC 
CGCATCAGCC GGCAGAACCA GCGCTCCATG TTTGACCACC TGAGCTACCT GCTGGAGAAC 
AGCGGCATCG GCCTGGGTAT GCAAGGCTCC ACGCCCCTGG ATGTGGCTGC CGCCTCCGTT 
ATCGACAACA ACGAGCTGGC CTTGGCGTTG CAGGAGCAGG ACCTGGAAAA GGTCGTGTCC 
TACCTGGCAG GCTGTGGCCT CCAAAGCTGC CCCATGCTCC TGGCCAAGGG CTACCCGGAC 
ATCGGCTGGA ACCCCTGCGG TGGGGAGCGC TACCTGGACT TCCTGCGCTT CGCTGTCTTT 
GTTAACGGCG AGAGCGTGGA GGAGAACGCC AACGTGGTGG TGCGGCTGCT CATCCGCAAA 
CCTGAGTGCT TCGGGCCTGC GCTGCGGGGT GAGGGCGGCT CGGGGCTGCT GGCCGCCATC 
GAGGAGGCCA TCCGCATCTC TGAGGACCCC GCGCGGGACG GCCCAGGCGT CCGCAGGGAC 
CGGCGGCGCG AGCACTTTGG GGAGGAGCCC CCTGAAGAAA ACCGCGTGCA CCTGGGCCAC 
GCCATCATGT CCTTCTATGC TGCCTTGATC GACCTGCTGG GCCGCTGTGC TCCGGAGATG 
CATCTGATCC AAGCCGGCAA GGGTGAGGCC CTGCGAATCC GCGCCATCCT TCGCTCCCTC 
GTGCCCCTGG ACGACCTCGT GGGCATCATC AGCCTCCCGC TGCAGATCCC CACCCTGGGC 
AAAGACGGGG CCCTGGTGCA GCCGAAGATG TCGGCCTCCT TCGTGCCGGA CCACAAGGCA 
TCCATGGTGC TCTTCCTGGA CCGCGTGTAC GGCATTGAGA ACCAGGACTT CCTGCTGCAC 
GTGCTGGACG TGGGGTTCCT TCCCGACATG AGAGCTGCCG CCTCGCTGGA CACGGCCACT 
TTCAGCACCA CGGAGATGGC GCTGGCGCTG AACCGCTACC TGTGCCTGGC TGTGCTGCCG 
CTCATCACCA AGTGTGCGCC GCTCTTTGCG GGCACGGAGC ACCGTGCCAT CATGGTGGAC 
TCGATGCTGC ACACCGTGTA CCGCCTGTCG CGCGGCCGCT CGCTCACCAA GGCGCAGCGC 
GACGTCATCG AGGACTGTCT CATGGCGCTG TGCAGGTACA TCCGCCCGTC CATGCTGCAG 
CACCTGCTGC GGCGCCTGGT GTTCGACGTG CCCATCCTGA ACGAGTTCGC CAAGATGCCG 
CTCAAGCTCC TCACCAACCA CTACGAGCGC TGCTGGAAGT ACTACTGCCT CCCCACGGGC 
TGGGCCAACT TCGGGGTCAC CTCGGAGGAG GAGCTGCACC TCACACGCAA ACTCTTCTGG 
GGCATCTTCG ACTCTCTGGC CCACAAGAAG TACGACCAGG AGCTGTACCG CATGGCCATG 
CCTTGTCTGT GCGCCATCGC GGGGGCCCTG CCTCCCGACT ACGTGGACGC TTCGTACTCT 
TCCAAGGCGG AGAAAAAGGC CACAGTGGAT GCTGAAGGCA ACTTTGATCC CCGGCCTGTG 
GAGACCCTCA ATGTGATCAT CCCGGAGAAG CTCGACTCCT TCATCAACAA GTTTGCCGAA 
TACACGCACG AGAAGTGGGC CTTCGACAAG ATCCAGAACA ACTGGTCGTA CGGAGAGAAC 
GTGGATGAGG AACTGAAGAC CCACCCCATG CTGAGGCCCT ACAAGACCTT TTCGGAGAAG 
GACAAAGAGA TCTACCGCTG GCCCATCAAG GAGTCCCTGA AGGCCATGAT TGCCTGGGAG 
TGGACGATCG AGAAGGCCAG GGAAGGCGAG GAGGAGAGGA CGGAGAAGAA AAAGACACGG 
AAGATTTCCC AGACTGCCCA GACCTACGAT CCCCGAGAAG GCTACAACCC GCAGCCCCCC 
GACCTCAGCG GAGTCACCCT GTCTCGGGAG TTGCAGGCCA TGGCCGAACA ACTGGCGGAA 
AACTACCATA ACACCTGGGG GAGAAAGAAG AAGCAGGAGC TGGAAGCCAA GGGCGGCGGG 
ACCCACCCCC TGCTGGTCCC CTACGACACG CTCACGGCCA AGGAGAAAGC GCGAGACCGA 
GAGAAGGCCC AGGAGCTGCT GAAGTTCCTG CAGATGAACG GCTACGCGGT CACCAGAGGC 
CTGAAGGACA TGGAGCTGGA CACGTCTTCC ATTGAGAAGC GGTTCGCCTT TGGCTTCCTG 
CAGCAGTTGC TGCGCTGGAT GGACATTTCG CAGGAGTTCA TTGCCCACCT GGAGGCTGTG 
GTCAGCAGTG GACGAGTGGA GAAGTCCCCA CACGAACAGG AGATTAAATT CTTTGCCAAG 
ATCCTGCTCC CTTTGATTAA CCAGTACTTC ACCAACCACT GCCTCTACTT CCTGTCCACA 
CCCGCCAAAG TGCTGGGCAG TGGTGGCCAT GCCTCCAACA AGGAGAAGGA GATGATCACC 
AGCCTCTTCT GCAAACTCGC TGCCCTTGTC CGCCACCGAG TCTCTCTCTT CGGCACAGAC 
GCCCCGGCTG TGGTCAACTG CCTTCACATC CTGGCACGCT CCCTGGATGC CAGGACGGTG 
ATGAAGTCGG GCCCCGAGAT CGTCAAGGCC GGCCTCCGCT CCTTCTTTGA GAGCGCCTCG 
GAGGACATCG AGAAGATGGT GGAGAACTTG CGGCTGGGCA AGGTGTCCCA GGCGCGCACG 
CAGGTGAAGG GCGTGGGCCA GAACCTCACC TACACCACCG TGGCCCTGCT GCCCGTCCTC 
ACCACGCTCT TCCAGCACAT CGCCCAGCAC CAGTTCGGCG ACGACGTCAT CCTGGATGAC 
GTGCAGGTGT CTTGCTACCG AACATTGTGC AGCATCTACT CTCTGGGGAC CACCAAGAAC 
ACTTATGTGG AAAAGCTACG GCCAGCCCTT GGAGAGTGCC TGGCCCGCCT GGCAGCGGCC 
ATGCCTGTGG CCTTCCTGGA GCCTCAGCTG AACGAGTACA ACGCTTGCTC CGTGTACACC 
ACCAAGTCTC CCCGGGAGCG AGCCATCCTG GGGCTCCCCA ACAGCGTGGA GGAGATGTGT 
CCTGACATCC CGGTGCTGGA TCGGCTCATG GCAGACATCG GGGGGCTGGC CGAGTCCGGG 
GCCCGCTACA CGGAGATGCC GCACGTCATC GAGATCACGC TGCCCATGCT CTGCAGCTAC 
CTGCCCCGCT GGTGGGAGCG CGGGCCCGAG GCGCCCCCTC CCGCCCTGCC TGCCGGCGCC 
CCCCCGCCCT GCACGGCTGT CACCTCCGAC CACCTCAACT CACTCTTGGG GAACATCTTG 
CGCATCATTG TCAACAACCT GGGCATTGAC GAGGCCACCT GGATGAAGCG GCTTGCTGTG 
TTCGCCCAGC CCATCGTGAG CCGGGCGCGG CCTGAGCTCC TGCATTCCCA CTTCATCCCC 
ACCATCGGGC GGCTGCGCAA GCGGGCAGGC AAGGTGGTGG CCGAGGAGGA GCAGCTGCGG 
CTGGAGGCCA AGGCCGAGGC CGAGGAGGGC GAGCTCCTGG TGCGGGACGA GTTCTCTGTG 
CTCTGCCGGG ACCTGTACGC CCTCTACCCG CTGCTCATCC GCTACGTGGA CAACAACAGG 
GCGCACTGGC TGACGGAGCC CAACGCCAAC GCCGAGGAGC TGTTCAGGAT GGTGGGCGAG 
ATCTTCATCT ACTGGTCCAA GTCCCACAAC TTCAAGCGTG AGGAGCAGAA CTTTGTGGTC 
CAGAACGAGA TCAACAACAT GTCCTTCCTG ACGGCTGACA GCAAGAGCAA GATGGCCAAG 
GCGGGAGACG CACAGTCGGG CGGCTCGGAC CAGGAACGCA CCAAGAAGAA GCGCCGGGGG 
GACAGGTACT CCGTGCAGAC GTCACTGATC GTGGCCACGC TGAAGAAGAT GCTGCCCATT 
GGCCTGAACA TGTGTGCGCC CACCGACCAG GACCTCATCA TGCTGGCCAA GACCCGCTAC 
GCCCTGAAAG ACACAGACGA GGAGGTCCGG GAATTTCTGC AGAACAACCT CCACCTTCAG 
GGAAAGGTCG AAGGCTCCCC GTCCCTGCGC TGGCAGATGG CGCTGTACCG GGGCCTCCCC 
GGCCGCGAGG AGGACGCCGA CGACCCGGAG AAAATCGTGC GCAGGGTCCA AGAAGTGTCA 
GCGGTGCTCT ATCACCTGGA ACAGACCGAG CACCCCTACA AGTCCAAGAA GGCCGTGTGG 
CACAAGCTGT TGTCCAAGCA GCGCCGGCGA GCCGTGGTGG CCTGCTTCCG TATGACGCCC 
CTGTACAACC TGCCCACGCA CCGGGCCTGT AACATGTTCT TGGAGAGCTA CAAAGCCGCC 
TGGATCCTGA CCGAAGATCA CAGTTTCGAG GACCGCATGA TAGATGATCT TTCAAAAGCT 
GGGGAGCAGG AGGAGGAGGA GGAGGAAGTG GAGGAGAAGA AGCCAGACCC CCTGCACCAG 
CTGGTCCTGC ACTTCAGCCG CACGGCCCTG ACTGAAAAGA GCAAACTGGA CGAGGACTAC 
CTGTACATGG CATATGCCGA CATCATGGCC AAGAGCTGCC ACCTGGAGGA GGGAGGGGAG 
AACGGTGAAG CTGAGGAAGA GGAGGTTGAG GTCTCCTTTG AGGAGAAAGA GATGGAGAAG 
CAGAGGCTCC TCTACCAGCA GTCACGGCTG CACACGCGCG GGGCCGCCGA GATGGTGCTG 
CAGATGATCA GCGCCTGCAA AGGAGAGACA GGTGCCATGG TGTCCTCCAC CCTGAAGCTG 
GGCATCTCCA TCCTCAATGG AGGCAATGCC GAGGTGCAGC AGAAAATGCT GGATTATCTG 
AAGGACAAGA AGGAAGTCGG CTTCTTCCAG AGCATCCAGG CGCTGATGCA GACATGCAGC 
GTCCTGGATC TCAATGCCTT CGAGAGACAG AACAAGGCAG AAGGCCTGGG CATGGTGAAC 
GAGGATGGAA CCGTCATCAA TCGCCAGAAC GGAGAGAAGG TTATGGCCGA TGACGAATTC 
ACACAAGATC TGTTCCGATT TCTGCAGCTG CTCTGTGAGG GGCACAACAA CGATTTCCAA 
AACTACTTAC GGACACAGAC GGGCAACACC ACCACGATTA ACATCATTAT CTGCACAGTG 
GACTACCTCC TGCGGCTGCA GGAGTCCATC AGCGACTTCT ACTGGTACTA CTCCGGCAAG 
GATGTCATTG AGGAGCAGGG CAAGAGGAAC TTCTCCAAGG CCATGTCCGT GGCCAAACAG 
GTGTTCAACA GCCTCACCGA GTACATCCAG GGCCCCTGCA CCGGGAACCA GCAGAGCCTG 
GCGCACAGCC GCCTCTGGGA CGCCGTGGTG GGATTCCTGC ACGTGTTTGC CCACATGATG 
ATGAAGCTCG CTCAGGACTC GAGCCAGATC GAGCTGTTGA AGGAGCTGCT GGACCTGCAG 
AAGGACATGG TGGTGATGCT GCTGTCCCTA CTGGAAGGGA ACGTGGTGAA CGGCATGATT 
GCCCGGCAGA TGGTGGACAT GCTGGTGGAG TCCTCGTCCA ACGTGGAGAT GATCCTCAAG 
TTCTTCGACA TGTTCCTGAA ACTCAAGGAC ATCGTGGGCT CCGAGGCCTT CCAGGACTAC 
GTCACCGATC CCCGTGGCCT CATCTCCAAA AAGGACTTCC AGAAGGCCAT GGACAGCCAG 
AAGCAGTTCA CTGGGCCGGA AATCCAGTTT CTGCTCTCGT GCTCCGAAGC CGACGAGAAT 
GAGATGATCA ACTTCGAGGA GTTCGCCAAC CGCTTCCAGG AGCCAGCCCG GGACATCGGC 
TTCAACGTGG CCGTGCTGCT GACCAACCTG TCGGAGCACG TGCCGCACGA CCCGCGCCTG 
CGCAACTTCC TGGAGCTGGC TGAGAGCATC CTTGAGTACT TCCGGCCCTA CCTGGGCCGC 
ATCGAGATCA TGGGCGCCTC CCGCCGCATC GAGCGCATCT ACTTCGAGAT CTCAGAGACC 
AACCGCGCCC AGTGGGAGAT GCCCCAGGTC AAGGAGTCCA AGCGCCAGTT CATCTTCGAC 
GTGGTCAACG AGGGCGGGGA GGCCGAGAAG ATGGAGCTGT TCGTGAGCTT CTGCGAGGAC 
ACCATCTTCG AGATGCAGAT CGCCGCGCAG ATCTCCGAGC CCGAGGGCGA GCCCGAGGCC 
GACGAGGACG AGGGCATGGG CGAGGCGGCC GCCGAGGGCG CCGAGGAGGG CGCGGCGGGC 
GCCGAGGGCG CGGCGGGCAC GGTGGCGGCG GGCGCCACGG CGCGGCTGGC GGCGGCCGCG 
GCCCGGGCGC TGCGCGGCCT CAGCTACCGC AGCCTGCGGC GGCGCGTGCG GCGGCTGCGG 
CGGCTCACGG CGCGCGAGGC GGCCACGGCG CTGGCCGCGC TGCTCTGGGC CGTGGTGGCG 
CGCGCGGGCG CGGCGGGCGC GGGCGCGGCG GCGGGCGCGC TGCGCCTGCT CTGGGGCTCG 
CTCTTCGGCG GCGGCCTGGT GGAGGGCGCC AAGAAGGTGA CGGTGACCGA GCTGCTGGCC 
GGCATGCCCG ATCCCACCAG CGACGAGGTG CACGGCGAGC AGCCGGCCGG GCCCGGCGGC 
GACGCGGACG GCGCGGGCGA GGGCGAAGGC GAGGGCGACG CGGCCGAGGG TGACGGCGAT 
GAGGAGGTGG CGGGTCACGA GGCCGGCCCG GGCGGTGCCG AGGGGGTCGT GGCCGTGGCC 
GACGGGGGCC CCTTCCGGCC CGAGGGCGCT GGCGGCCTTG GGGACATGGG CGACACCACG 
CCGGCGGAAC CGCCCACGCC CGAGGGCTCC CCGATCCTCA AGAGGAAGCT GGGGGTGGAT 
GGAGAAGAGG AGGAGCTGGT TCCAGAACCG GAGCCCGAGC CCGAGCCTGA GCCTGAGAAA 
GCCGATGAGG AGAACGGGGA GAAGGAGGAA GTCCCCGAGG CCCCACCAGA ACCCCCCAAG 
AAAGCCCCTC CCTCACCTCC TGCAAAGAAG GAGGAAGCCG GAGGCGCCGG CATGGAGTTC 
TGGGGAGAAC TGGAAGTGCA GAGGGTGAAG TTCTTGAACT ACCTCTCCCG GAACTTCTAC 
ACCCTGCGCT TCCTTGCCCT CTTCTTGGCG TTTGCCATCA ACTTCATCCT GCTGTTTTAT 
AAGGTCTCAG ACTCTCCACC AGGGGAGGAT GACATGGAGG GCTCGGCAGC AGGGGACCTG 
GCGGGTGCAG GGTCCGGGGG CGGCTCCGGC TGGGGCTCGG GGGCCGGCGA GGAGGCAGAG 
GGGGACGAGG ACGAGAACAT GGTATACTAC TTCCTGGAGG AGAGCACGGG CTACATGGAG 
CCGGCCCTGT GGTGCCTGAG CCTGCTGCAC ACGCTGGTGG CCTTCCTCTG CATCATCGGC 
TACAACTGCC TCAAGGTGCC CCTGGTGATC TTCAAGCGGG AGAAGGAGCT GGCACGAAAG 
CTGGAGTTTG ACGGCCTGTA CATCACAGAG CAGCCTGGGG ACGACGACGT GAAGGGCCAG 
TGGGACCGGC TGGTGCTCAA CACGCCGTCT TTCCCCAGCA ACTACTGGGA CAAGTTTGTC 
AAGCGGAAGG TCCTGGACAA ACACGGGGAC ATCTTCGGGC GGGAGCGGAT TGCCGAGCTG 
CTGGGCATGG ACCTGGCCTC GCTGGAGATC ACGGCCCACA ACGAGCGCAA GCCCGACCCC 
CCGCCGGGGC TGCTGACCTG GCTCATGTCC ATCGATGTCA AGTACCAGAT CTGGAAGTTC 
GGGGTCATCT TCACGGACAA CTCGTTCCTG TACCTGGGCT GGTACATGGT GATGTCCCTC 
CTGGGCCACT ACAACAACTT CTTCTTTGCC GCCCACCTGC TGGACATCGC CATGGGGGTC 
AAGACGCTGC GTACCATCCT CTCCTCTGTC ACCCACAATG GGAAACAGCT GGTGATGACT 
GTGGGCCTCC TGGCCGTCGT GGTCTACCTG TACACTGTGG TGGCCTTCAA CTTCTTCCGC 
AAGTTCTACA ACAAGAGCGA GGACGAGGAC GAGCCGGACA TGAAGTGCGA TGACATGATG 
ACGTGCTACC TGTTCCACAT GTACGTGGGC GTCCGGGCTG GCGGAGGCAT CGGGGACGAG 
ATCGAGGACC CAGCGGGCGA TGAATACGAG CTCTACCGGG TGGTCTTCGA CATCACCTTC 
TTCTTCTTCG TCATTGTCAT CCTGCTGGCC ATCATCCAGG GTCTGATTAT CGACGCCTTC 
GGCGAGCTCC GAGACCAGCA GGAGCAAGTG AAGGAAGATA TGGAGACCAA ATGCTTCATC 
TGCGGGATTG GCAGTGACTA CTTCGATACC ACGCCGCACG GCTTCGAGAC CCACACGCTA 
GAGGAGCACA ATCTGGCCAA TTACATGTTC TTCTTGATGT ATCTGATAAA CAAGGACGAG 
ACGGAGCACA CGGGCCAGGA GTCCTACGTC TGGAAGATGT ATCAGGAGAG GTGCTGGGAC 
TTCTTCCCCG CCGGCGACTG CTTCCGCAAG CAGTACGAGG ACCAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ion-pulling simulations provide insights into the mechanisms of channel opening of the skeletal muscle ryanodine receptor.
The Journal of biological chemistry292(31)12947-12958(2017 08)
Mowrey DD,Xu L,Mei Y,Pasek DA,Meissner G,Dokholyan NV

Reduced threshold for store overload-induced Ca
The Biochemical journal474(16)2749-2761(2017 08)
Chen W,Koop A,Liu Y,Guo W,Wei J,Wang R,MacLennan DH,Dirksen RT,Chen SRW

The Central domain of RyR1 is the transducer for long-range allosteric gating of channel opening.
Cell research26(9)995-1006(2016 09)
Bai XC,Yan Z,Wu J,Li Z,Yan N

Structural insights into Ca(2+)-activated long-range allosteric channel gating of RyR1.
Cell research26(9)977-94(2016 09)
Wei R,Wang X,Zhang Y,Mukherjee S,Zhang L,Chen Q,Huang X,Jing S,Liu C,Li S,Wang G,Xu Y,Zhu S,Williams AJ,Sun F,Yin CC

Channel Gating Dependence on Pore Lining Helix Glycine Residues in Skeletal Muscle Ryanodine Receptor.
The Journal of biological chemistry290(28)17535-45(2015 Jul)
Mei Y,Xu L,Mowrey DD,Mendez Giraldez R,Wang Y,Pasek DA,Dokholyan NV,Meissner G

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RYR1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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