HBB2 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	HBB2
Entrez Gene ID	100009084
Full Name	hemoglobin, beta
Synonyms	HBB,HBB1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)
Summary	The adult hemoglobin is a heterotetramer of two alpha chains and two beta chains, and functions in the transport of oxygen from the lungs to various peripheral tissues. This gene is expressed in red blood cells and encodes the beta polypeptide chain. Studies have shown that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, both in rabbit reticulocyte lysates and in intact reticulocytes. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001314043.1	NP_001300972.1	hemoglobin subunit beta-1/2 isoform 1x
NM_001082260.3	NP_001075729.2	hemoglobin subunit beta-1/2 isoform 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative analysis of the heme iron electronic structure and stereochemistry in tetrameric rabbit hemoglobin and monomeric soybean leghemoglobin a using Mössbauer spectroscopy with a high velocity resolution.
Alenkina IV, Kumar A, Berkovsky AL, Oshtrakh MI
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy191547-557(2018 Feb)

Rabbit beta-globin is extended beyond its UGA stop codon by multiple suppressions and translational reading gaps.
Chittum HS, Lane WS, Carlson BA, Roller PP, Lung FD, Lee BJ, Hatfield DL
Biochemistry37(31)10866-70(1998 Aug)

Secondary structure of mouse and rabbit alpha- and beta-globin mRNAs: differential accessibility of alpha and beta initiator AUG codons towards nucleases.
Pavlakis GN, Lockard RE, Vamvakopoulos N, Rieser L, RajBhandary UL, Vournakis JN
Cell19(1)91-102(1980 Jan)

A UGA termination suppression tRNATrp active in rabbit reticulocytes.
Geller AI, Rich A
Nature283(5742)41-6(1980 Jan)

Haemoglobins, XVII. The primary structure of the beta-chain of rabbit haemoglobin.
Best JS, Flamm U, Braunitzer G
Hoppe-Seyler's Zeitschrift fur physiologische Chemie350(5)563-80(1969 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HBB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb41860	NM_001314043.1 Latest version!	Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OOb41862	NM_001082260.3 Latest version!	Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HBB2 ( NM_001314043.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001300972.1 Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OOb41860
Clone ID Related Accession (Same CDS sequence)	NM_001314043.1
Accession Version	NM_001314043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Oryctolagus cuniculus(rabbit)
Product	hemoglobin subunit beta-1/2 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GBCI01005570.1 and EB379014.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon (nt 707-709), while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAA termination codon (nt 776-778). This RefSeq represents the longer, C-terminally extended isoform. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN890520.1, M10843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1876840, SAMN00013645 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 9692979 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGCATC TGTCCAGTGA GGAGAAGTCT GCGGTCACTG CCCTGTGGGG CAAGGTGAAT 
GTGGAAGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTCG AGTCCTTTGG GGACCTGTCC TCTGCACATG CTGTTATGAG CAATCCTAAG 
GTGAAGGCTC ATGGCAAGAA GGTGCTGGCT GCCTTCAGTG AGGGTCTGAA TCACCTGGAC 
AACCTCAAAG GCACCTTTGC TAAGCTGAGT GAACTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTTGTTG TGCTGTCTCA TCATTTTGGC 
AAAGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT 
GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC 
ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300972.1
CDS	266..778
Translation	MVHLSSEEKSAVTALWGKVNVEEVGGEALGRLLVVYPWTQRFFESFGDLSSAHAVMSNPKVKAHGKKVLAAFSEGLNHLDNLKGTFAKLSELHCDKLHVDPENFRLLGNVLVVVLSHHFGKEFTPQVQAAYQKVVAGVANALAHKYH*DLFPSAKNYGDIMKPLEHLTSG

Target ORF information:

RefSeq Version	NM_001314043.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314043.1

ORF Insert Sequence:

ATGGTGCATC TGTCCAGTGA GGAGAAGTCT GCGGTCACTG CCCTGTGGGG CAAGGTGAAT 
GTGGAAGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTCG AGTCCTTTGG GGACCTGTCC TCTGCACATG CTGTTATGAG CAATCCTAAG 
GTGAAGGCTC ATGGCAAGAA GGTGCTGGCT GCCTTCAGTG AGGGTCTGAA TCACCTGGAC 
AACCTCAAAG GCACCTTTGC TAAGCTGAGT GAACTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTTGTTG TGCTGTCTCA TCATTTTGGC 
AAAGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT 
GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC 
ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HBB2 ( NM_001082260.3 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001075729.2 Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OOb41862
Clone ID Related Accession (Same CDS sequence)	NM_001082260.3
Accession Version	NM_001082260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Oryctolagus cuniculus(rabbit)
Product	hemoglobin subunit beta-1/2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GBCI01005570.1 and EB379014.1. On Sep 29, 2015 this sequence version replaced NM_001082260.2. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon (nt 707-709), while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAA termination codon (nt 776-778). This RefSeq represents the shorter isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN890520.1, M10843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1876840, SAMN00013645 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCATC TGTCCAGTGA GGAGAAGTCT GCGGTCACTG CCCTGTGGGG CAAGGTGAAT 
GTGGAAGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTCG AGTCCTTTGG GGACCTGTCC TCTGCACATG CTGTTATGAG CAATCCTAAG 
GTGAAGGCTC ATGGCAAGAA GGTGCTGGCT GCCTTCAGTG AGGGTCTGAA TCACCTGGAC 
AACCTCAAAG GCACCTTTGC TAAGCTGAGT GAACTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTTGTTG TGCTGTCTCA TCATTTTGGC 
AAAGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT 
GCCCTGGCTC ACAAATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075729.2
CDS	266..709
Translation	MVHLSSEEKSAVTALWGKVNVEEVGGEALGRLLVVYPWTQRFFESFGDLSSAHAVMSNPKVKAHGKKVLAAFSEGLNHLDNLKGTFAKLSELHCDKLHVDPENFRLLGNVLVVVLSHHFGKEFTPQVQAAYQKVVAGVANALAHKYH

Target ORF information:

RefSeq Version	NM_001082260.3
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus hemoglobin, beta (HBB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082260.3

ORF Insert Sequence:

ATGGTGCATC TGTCCAGTGA GGAGAAGTCT GCGGTCACTG CCCTGTGGGG CAAGGTGAAT 
GTGGAAGAAG TTGGTGGTGA GGCCCTGGGC AGGCTGCTGG TTGTCTACCC ATGGACCCAG 
AGGTTCTTCG AGTCCTTTGG GGACCTGTCC TCTGCACATG CTGTTATGAG CAATCCTAAG 
GTGAAGGCTC ATGGCAAGAA GGTGCTGGCT GCCTTCAGTG AGGGTCTGAA TCACCTGGAC 
AACCTCAAAG GCACCTTTGC TAAGCTGAGT GAACTGCACT GTGACAAGCT GCACGTGGAT 
CCTGAGAACT TCAGGCTCCT GGGCAACGTG CTGGTTGTTG TGCTGTCTCA TCATTTTGGC 
AAAGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG TGTGGCCAAT 
GCCCTGGCTC ACAAATACCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative analysis of the heme iron electronic structure and stereochemistry in tetrameric rabbit hemoglobin and monomeric soybean leghemoglobin a using Mössbauer spectroscopy with a high velocity resolution.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy191547-557(2018 Feb)
Alenkina IV,Kumar A,Berkovsky AL,Oshtrakh MI

Rabbit beta-globin is extended beyond its UGA stop codon by multiple suppressions and translational reading gaps.
Biochemistry37(31)10866-70(1998 Aug)
Chittum HS,Lane WS,Carlson BA,Roller PP,Lung FD,Lee BJ,Hatfield DL

Secondary structure of mouse and rabbit alpha- and beta-globin mRNAs: differential accessibility of alpha and beta initiator AUG codons towards nucleases.
Cell19(1)91-102(1980 Jan)
Pavlakis GN,Lockard RE,Vamvakopoulos N,Rieser L,RajBhandary UL,Vournakis JN

A UGA termination suppression tRNATrp active in rabbit reticulocytes.
Nature283(5742)41-6(1980 Jan)
Geller AI,Rich A

Haemoglobins, XVII. The primary structure of the beta-chain of rabbit haemoglobin.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie350(5)563-80(1969 May)
Best JS,Flamm U,Braunitzer G