LOC110529424 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110529424
Entrez Gene ID	110529424
Full Name	melanoma inhibitory activity protein 3-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021611581.1	XP_021467256.1	melanoma inhibitory activity protein 3-like isoform X1
XM_021611582.1	XP_021467257.1	melanoma inhibitory activity protein 3-like isoform X2
XM_021611584.1	XP_021467259.1	melanoma inhibitory activity protein 3-like isoform X4
XM_021611583.1	XP_021467258.1	melanoma inhibitory activity protein 3-like isoform X3
XM_021611585.1	XP_021467260.1	melanoma inhibitory activity protein 3-like isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC110529424 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110529424 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf04238	XM_021611581.1 Latest version!	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf04239	XM_021611582.1 Latest version!	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf04240	XM_021611583.1 Latest version!	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf04241	XM_021611584.1 Latest version!	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf04242	XM_021611585.1 Latest version!	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110529424 ( XM_021611581.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467256.1 Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf04238
Clone ID Related Accession (Same CDS sequence)	XM_021611581.1
Accession Version	XM_021611581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	melanoma inhibitory activity protein 3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGCAGACCG CTCCACCACA GGCTGAGGCC CAGGCCTCCA CAGAGAACCC AGAGGCAAAC 
TCAGGACCTC GGGGCCCTGG CTCCCTCCTG GTCTCTCCTA TCAGCTCCCC CCCCGACTCA 
GGCCCCGGCC CCCTCAGACC AGTAATCAGC CATCCCTCCG GACTCTGCTA CCGTCCTCCT 
GGCCCTGGGC CCCACCACAT CCCTCCTCCC TCACCCTTCA TGCCTCCCAT GTACCGACCA 
GCCAACGGAC ACCCAGGCAT GATGCCTCCC GGACCCCCAC CACCTAATGG ACACCCACCT 
GGCCCCATGA TGCCACCCGG ACAGCGGCCT CCACCTCCTG GTGCCTACGG CCCCCCACAC 
CACCGGGGCC CTCCTCCACC TCACTATGGA CCAGTGCCTC CCCCATTCGG TGTTCGCGGC 
GGCCCCCCTA TGGCCCGACC CATGGGACCA CCACGTCCCT ACATGCACTA CGGACCACGT 
GATCACTCCA TCCCACCCCA GCACCTGCCC CCAGGAGCGG CCCCATACCC TCCTCACAGT 
GGCCCCAGAG ACTTCCCCGG GCAGCCCCCC ATGCAGCAGG CCCCCCATGG GCATGACTCT 
TCTGTGGGTC CCCAGGGGCA AGACTACAGC TCCCAGCAGG CAGCAGCTAC CCCCCAGGAC 
TCAGTCAGCT CCTCCATGGG AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467256.1
CDS	381..6170
Translation	MAVNVSHVYILLLFIINFISNAATERRFSDFKRCADEECSMLLCRGKASSDFTGPDCRFLSFKKGETVYVYYKLSGRRTDIWAGTVGSRFGYFHKDQLAINHIYTEKELEIPAQETDFVCFDTGHDKFDSYDIESLLVSSLLLTDQDKSVQGTTETLDRNKSAESTTVEVDEPPPEVTLLENNDVDSDVPEDIDKVLEEVLEEFPEDKDPGHFDPLESSLPQPEIETLDAKGVESNTVVEPTAERIKDDLQKYQLTTEDSVPDSVVEPEQGETKESLSEPLSKDDPELENAPDGFSEGRPVPQLKTTLGTTFDAVTSDDEETRKVTPYDEEDEGSEEFEYQQDDKSDYHLRETPLLAFSEEHSNLEHEDIDKVLEVLEDKDPGHFESDQTDEEESPPETAEKQDSKDKNLWSSLGDTVFNIVSGGERTAHVTGSEEEDDDDDDDEGEIVPEELPKIEEPKENIQLLANDPKQKPEVTEQPHEVIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPLADEVIPFEHTEEAIAENLTVNESPVPKHVSQTEMLSDFDSNTIDSEQTQAVEELPIQKEPKENIQLLANDPQQEPEVTEQPHEDIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPPADEVIPFEHTEEALAENLTVNESPFPKHVTQTELLSDFDSKTNVSEQKQAIELPIQKESRDFSQVENKLKLGGTELFRRFRGPNVPDPTKNTMVKERHEELPVDNEDLIDPENLEIEEELLEDENAALSSSKTEHTDKDKESRSEFGSEQSNYATETEVSSDELDVVQIEEAIDIEPEEHDIEDSESAGLDQTHTPSLEDKVPDPLSGQEPEYSDNVLRLTLLRNHFKEQDMKLFQKILGLQNLFRVEFLFSDLEQELNAALMSQTNTSEDIEKVLEAILEASETPILDEIERMLDARENADVQQETGELDVEATILDNFQELAFTLHQKYSMTSDSAPLAEGSQLHPDTDGDVSDAEEEKMFLHSVGDINEDNLTVTGEETKAPEEKPDHEGHNRPDLDMGLEEDVGHFNRNQDNQPEEIQRGPQAILENTLDMGLVDMEHPSSGSLESPPVSDFQEEEQSSSSIVSVLIFSGSLVTVVYEYLGIYAVMMVTCLPEEWKPGPDFYGVSWEPVLATAGAGFIGFLFLFWRSVLSVKRKSYLTTEKEMADRMKTFEQEKEEILLKVAELQQQGEELKEKKKLSEKSATSSLEKIQELKNLVQEMERQNECLDEEKNLLARSFDEERTKAAKHQDMMSEMDKTIEKLKRSRKKTQEALSKATILMEEAKLREDARNVQHHVLEKDIATLREENISLHHAANSWEEKHRELSEQIKVYQKSQKDLEDSLAQKDHNVEVLSDLLGDLEACDDLKGGVVANGEAANDKQTVIRNRIKQMMDVTRVQTTLSVVEEERDRFMTKLLNEEKARKELEEQYQKLEHAILLVKSDKNHLENQYKTLQQKNEIITEMYQQKENALQQKLTKEEFERRNNEDRLTEVDGKALEAEEEVKVCRQRIKEIQDELKQTEKSYKAQIIEQEQKSHENWVIARAAERALLDEKKESTNLRIKLTEMSSKLNELRRPLFKPTPGMAPMPLRRGDSYGPSPVSGGAPSPPLMMEGPGRPPSAPVGRRNEPYGPRPPSDPHGRYPPDHKHPVAARPDTIAPRTSSPSSLDSSQTAPPQAEAQASTENPEANSGPRGPGSLLVSPISSPPDSGPGPLRPVISHPSGLCYRPPGPGPHHIPPPSPFMPPMYRPANGHPGMMPPGPPPPNGHPPGPMMPPGQRPPPPGAYGPPHHRGPPPPHYGPVPPPFGVRGGPPMARPMGPPRPYMHYGPRDHSIPPQHLPPGAAPYPPHSGPRDFPGQPPMQQAPHGHDSSVGPQGQDYSSQQAAATPQDSVSSSMGEP

Target ORF information:

RefSeq Version	XM_021611581.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611581.1

ORF Insert Sequence:

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGCAGACCG CTCCACCACA GGCTGAGGCC CAGGCCTCCA CAGAGAACCC AGAGGCAAAC 
TCAGGACCTC GGGGCCCTGG CTCCCTCCTG GTCTCTCCTA TCAGCTCCCC CCCCGACTCA 
GGCCCCGGCC CCCTCAGACC AGTAATCAGC CATCCCTCCG GACTCTGCTA CCGTCCTCCT 
GGCCCTGGGC CCCACCACAT CCCTCCTCCC TCACCCTTCA TGCCTCCCAT GTACCGACCA 
GCCAACGGAC ACCCAGGCAT GATGCCTCCC GGACCCCCAC CACCTAATGG ACACCCACCT 
GGCCCCATGA TGCCACCCGG ACAGCGGCCT CCACCTCCTG GTGCCTACGG CCCCCCACAC 
CACCGGGGCC CTCCTCCACC TCACTATGGA CCAGTGCCTC CCCCATTCGG TGTTCGCGGC 
GGCCCCCCTA TGGCCCGACC CATGGGACCA CCACGTCCCT ACATGCACTA CGGACCACGT 
GATCACTCCA TCCCACCCCA GCACCTGCCC CCAGGAGCGG CCCCATACCC TCCTCACAGT 
GGCCCCAGAG ACTTCCCCGG GCAGCCCCCC ATGCAGCAGG CCCCCCATGG GCATGACTCT 
TCTGTGGGTC CCCAGGGGCA AGACTACAGC TCCCAGCAGG CAGCAGCTAC CCCCCAGGAC 
TCAGTCAGCT CCTCCATGGG AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110529424 ( XM_021611582.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467257.1 Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X2, mRNA.

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CloneID	OOf04239
Clone ID Related Accession (Same CDS sequence)	XM_021611582.1
Accession Version	XM_021611582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	melanoma inhibitory activity protein 3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGACCGCTC CACCACAGGC TGAGGCCCAG GCCTCCACAG AGAACCCAGA GGCAAACTCA 
GGACCTCGGG GCCCTGGCTC CCTCCTGGTC TCTCCTATCA GCTCCCCCCC CGACTCAGGC 
CCCGGCCCCC TCAGACCAGT AATCAGCCAT CCCTCCGGAC TCTGCTACCG TCCTCCTGGC 
CCTGGGCCCC ACCACATCCC TCCTCCCTCA CCCTTCATGC CTCCCATGTA CCGACCAGCC 
AACGGACACC CAGGCATGAT GCCTCCCGGA CCCCCACCAC CTAATGGACA CCCACCTGGC 
CCCATGATGC CACCCGGACA GCGGCCTCCA CCTCCTGGTG CCTACGGCCC CCCACACCAC 
CGGGGCCCTC CTCCACCTCA CTATGGACCA GTGCCTCCCC CATTCGGTGT TCGCGGCGGC 
CCCCCTATGG CCCGACCCAT GGGACCACCA CGTCCCTACA TGCACTACGG ACCACGTGAT 
CACTCCATCC CACCCCAGCA CCTGCCCCCA GGAGCGGCCC CATACCCTCC TCACAGTGGC 
CCCAGAGACT TCCCCGGGCA GCCCCCCATG CAGCAGGCCC CCCATGGGCA TGACTCTTCT 
GTGGGTCCCC AGGGGCAAGA CTACAGCTCC CAGCAGGCAG CAGCTACCCC CCAGGACTCA 
GTCAGCTCCT CCATGGGAGA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467257.1
CDS	382..6168
Translation	MAVNVSHVYILLLFIINFISNAATERRFSDFKRCADEECSMLLCRGKASSDFTGPDCRFLSFKKGETVYVYYKLSGRRTDIWAGTVGSRFGYFHKDQLAINHIYTEKELEIPAQETDFVCFDTGHDKFDSYDIESLLVSSLLLTDQDKSVQGTTETLDRNKSAESTTVEVDEPPPEVTLLENNDVDSDVPEDIDKVLEEVLEEFPEDKDPGHFDPLESSLPQPEIETLDAKGVESNTVVEPTAERIKDDLQKYQLTTEDSVPDSVVEPEQGETKESLSEPLSKDDPELENAPDGFSEGRPVPQLKTTLGTTFDAVTSDDEETRKVTPYDEEDEGSEEFEYQQDDKSDYHLRETPLLAFSEEHSNLEHEDIDKVLEVLEDKDPGHFESDQTDEEESPPETAEKQDSKDKNLWSSLGDTVFNIVSGGERTAHVTGSEEEDDDDDDDEGEIVPEELPKIEEPKENIQLLANDPKQKPEVTEQPHEVIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPLADEVIPFEHTEEAIAENLTVNESPVPKHVSQTEMLSDFDSNTIDSEQTQAVEELPIQKEPKENIQLLANDPQQEPEVTEQPHEDIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPPADEVIPFEHTEEALAENLTVNESPFPKHVTQTELLSDFDSKTNVSEQKQAIELPIQKESRDFSQVENKLKLGGTELFRRFRGPNVPDPTKNTMVKERHEELPVDNEDLIDPENLEIEEELLEDENAALSSSKTEHTDKDKESRSEFGSEQSNYATETEVSSDELDVVQIEEAIDIEPEEHDIEDSESAGLDQTHTPSLEDKVPDPLSGQEPEYSDNVLRLTLLRNHFKEQDMKLFQKILGLQNLFRVEFLFSDLEQELNAALMSQTNTSEDIEKVLEAILEASETPILDEIERMLDARENADVQQETGELDVEATILDNFQELAFTLHQKYSMTSDSAPLAEGSQLHPDTDGDVSDAEEEKMFLHSVGDINEDNLTVTGEETKAPEEKPDHEGHNRPDLDMGLEEDVGHFNRNQDNQPEEIQRGPQAILENTLDMGLVDMEHPSSGSLESPPVSDFQEEEQSSSSIVSVLIFSGSLVTVVYEYLGIYAVMMVTCLPEEWKPGPDFYGVSWEPVLATAGAGFIGFLFLFWRSVLSVKRKSYLTTEKEMADRMKTFEQEKEEILLKVAELQQQGEELKEKKKLSEKSATSSLEKIQELKNLVQEMERQNECLDEEKNLLARSFDEERTKAAKHQDMMSEMDKTIEKLKRSRKKTQEALSKATILMEEAKLREDARNVQHHVLEKDIATLREENISLHHAANSWEEKHRELSEQIKVYQKSQKDLEDSLAQKDHNVEVLSDLLGDLEACDDLKGGVVANGEAANDKQTVIRNRIKQMMDVTRVQTTLSVVEEERDRFMTKLLNEEKARKELEEQYQKLEHAILLVKSDKNHLENQYKTLQQKNEIITEMYQQKENALQQKLTKEEFERRNNEDRLTEVDGKALEAEEEVKVCRQRIKEIQDELKQTEKSYKAQIIEQEQKSHENWVIARAAERALLDEKKESTNLRIKLTEMSSKLNELRRPLFKPTPGMAPMPLRRGDSYGPSPVSGGAPSPPLMMEGPGRPPSAPVGRRNEPYGPRPPSDPHGRYPPDHKHPVAARPDTIAPRTSSPSSLDSSTAPPQAEAQASTENPEANSGPRGPGSLLVSPISSPPDSGPGPLRPVISHPSGLCYRPPGPGPHHIPPPSPFMPPMYRPANGHPGMMPPGPPPPNGHPPGPMMPPGQRPPPPGAYGPPHHRGPPPPHYGPVPPPFGVRGGPPMARPMGPPRPYMHYGPRDHSIPPQHLPPGAAPYPPHSGPRDFPGQPPMQQAPHGHDSSVGPQGQDYSSQQAAATPQDSVSSSMGEP

Target ORF information:

RefSeq Version	XM_021611582.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611582.1

ORF Insert Sequence:

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGACCGCTC CACCACAGGC TGAGGCCCAG GCCTCCACAG AGAACCCAGA GGCAAACTCA 
GGACCTCGGG GCCCTGGCTC CCTCCTGGTC TCTCCTATCA GCTCCCCCCC CGACTCAGGC 
CCCGGCCCCC TCAGACCAGT AATCAGCCAT CCCTCCGGAC TCTGCTACCG TCCTCCTGGC 
CCTGGGCCCC ACCACATCCC TCCTCCCTCA CCCTTCATGC CTCCCATGTA CCGACCAGCC 
AACGGACACC CAGGCATGAT GCCTCCCGGA CCCCCACCAC CTAATGGACA CCCACCTGGC 
CCCATGATGC CACCCGGACA GCGGCCTCCA CCTCCTGGTG CCTACGGCCC CCCACACCAC 
CGGGGCCCTC CTCCACCTCA CTATGGACCA GTGCCTCCCC CATTCGGTGT TCGCGGCGGC 
CCCCCTATGG CCCGACCCAT GGGACCACCA CGTCCCTACA TGCACTACGG ACCACGTGAT 
CACTCCATCC CACCCCAGCA CCTGCCCCCA GGAGCGGCCC CATACCCTCC TCACAGTGGC 
CCCAGAGACT TCCCCGGGCA GCCCCCCATG CAGCAGGCCC CCCATGGGCA TGACTCTTCT 
GTGGGTCCCC AGGGGCAAGA CTACAGCTCC CAGCAGGCAG CAGCTACCCC CCAGGACTCA 
GTCAGCTCCT CCATGGGAGA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110529424 ( XM_021611583.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467258.1 Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf04240
Clone ID Related Accession (Same CDS sequence)	XM_021611583.1
Accession Version	XM_021611583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	melanoma inhibitory activity protein 3-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGAACTCAG GACCTCGGGG CCCTGGCTCC CTCCTGGTCT CTCCTATCAG CTCCCCCCCC 
GACTCAGGCC CCGGCCCCCT CAGACCAGTA ATCAGCCATC CCTCCGGACT CTGCTACCGT 
CCTCCTGGCC CTGGGCCCCA CCACATCCCT CCTCCCTCAC CCTTCATGCC TCCCATGTAC 
CGACCAGCCA ACGGACACCC AGGCATGATG CCTCCCGGAC CCCCACCACC TAATGGACAC 
CCACCTGGCC CCATGATGCC ACCCGGACAG CGGCCTCCAC CTCCTGGTGC CTACGGCCCC 
CCACACCACC GGGGCCCTCC TCCACCTCAC TATGGACCAG TGCCTCCCCC ATTCGGTGTT 
CGCGGCGGCC CCCCTATGGC CCGACCCATG GGACCACCAC GTCCCTACAT GCACTACGGA 
CCACGTGATC ACTCCATCCC ACCCCAGCAC CTGCCCCCAG GAGCGGCCCC ATACCCTCCT 
CACAGTGGCC CCAGAGACTT CCCCGGGCAG CCCCCCATGC AGCAGGCCCC CCATGGGCAT 
GACTCTTCTG TGGGTCCCCA GGGGCAAGAC TACAGCTCCC AGCAGGCAGC AGCTACCCCC 
CAGGACTCAG TCAGCTCCTC CATGGGAGAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467258.1
CDS	381..6116
Translation	MAVNVSHVYILLLFIINFISNAATERRFSDFKRCADEECSMLLCRGKASSDFTGPDCRFLSFKKGETVYVYYKLSGRRTDIWAGTVGSRFGYFHKDQLAINHIYTEKELEIPAQETDFVCFDTGHDKFDSYDIESLLVSSLLLTDQDKSVQGTTETLDRNKSAESTTVEVDEPPPEVTLLENNDVDSDVPEDIDKVLEEVLEEFPEDKDPGHFDPLESSLPQPEIETLDAKGVESNTVVEPTAERIKDDLQKYQLTTEDSVPDSVVEPEQGETKESLSEPLSKDDPELENAPDGFSEGRPVPQLKTTLGTTFDAVTSDDEETRKVTPYDEEDEGSEEFEYQQDDKSDYHLRETPLLAFSEEHSNLEHEDIDKVLEVLEDKDPGHFESDQTDEEESPPETAEKQDSKDKNLWSSLGDTVFNIVSGGERTAHVTGSEEEDDDDDDDEGEIVPEELPKIEEPKENIQLLANDPKQKPEVTEQPHEVIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPLADEVIPFEHTEEAIAENLTVNESPVPKHVSQTEMLSDFDSNTIDSEQTQAVEELPIQKEPKENIQLLANDPQQEPEVTEQPHEDIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPPADEVIPFEHTEEALAENLTVNESPFPKHVTQTELLSDFDSKTNVSEQKQAIELPIQKESRDFSQVENKLKLGGTELFRRFRGPNVPDPTKNTMVKERHEELPVDNEDLIDPENLEIEEELLEDENAALSSSKTEHTDKDKESRSEFGSEQSNYATETEVSSDELDVVQIEEAIDIEPEEHDIEDSESAGLDQTHTPSLEDKVPDPLSGQEPEYSDNVLRLTLLRNHFKEQDMKLFQKILGLQNLFRVEFLFSDLEQELNAALMSQTNTSEDIEKVLEAILEASETPILDEIERMLDARENADVQQETGELDVEATILDNFQELAFTLHQKYSMTSDSAPLAEGSQLHPDTDGDVSDAEEEKMFLHSVGDINEDNLTVTGEETKAPEEKPDHEGHNRPDLDMGLEEDVGHFNRNQDNQPEEIQRGPQAILENTLDMGLVDMEHPSSGSLESPPVSDFQEEEQSSSSIVSVLIFSGSLVTVVYEYLGIYAVMMVTCLPEEWKPGPDFYGVSWEPVLATAGAGFIGFLFLFWRSVLSVKRKSYLTTEKEMADRMKTFEQEKEEILLKVAELQQQGEELKEKKKLSEKSATSSLEKIQELKNLVQEMERQNECLDEEKNLLARSFDEERTKAAKHQDMMSEMDKTIEKLKRSRKKTQEALSKATILMEEAKLREDARNVQHHVLEKDIATLREENISLHHAANSWEEKHRELSEQIKVYQKSQKDLEDSLAQKDHNVEVLSDLLGDLEACDDLKGGVVANGEAANDKQTVIRNRIKQMMDVTRVQTTLSVVEEERDRFMTKLLNEEKARKELEEQYQKLEHAILLVKSDKNHLENQYKTLQQKNEIITEMYQQKENALQQKLTKEEFERRNNEDRLTEVDGKALEAEEEVKVCRQRIKEIQDELKQTEKSYKAQIIEQEQKSHENWVIARAAERALLDEKKESTNLRIKLTEMSSKLNELRRPLFKPTPGMAPMPLRRGDSYGPSPVSGGAPSPPLMMEGPGRPPSAPVGRRNEPYGPRPPSDPHGRYPPDHKHPVAARPDTIAPRTSSPSSLDSSNSGPRGPGSLLVSPISSPPDSGPGPLRPVISHPSGLCYRPPGPGPHHIPPPSPFMPPMYRPANGHPGMMPPGPPPPNGHPPGPMMPPGQRPPPPGAYGPPHHRGPPPPHYGPVPPPFGVRGGPPMARPMGPPRPYMHYGPRDHSIPPQHLPPGAAPYPPHSGPRDFPGQPPMQQAPHGHDSSVGPQGQDYSSQQAAATPQDSVSSSMGEP

Target ORF information:

RefSeq Version	XM_021611583.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611583.1

ORF Insert Sequence:

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGAACTCAG GACCTCGGGG CCCTGGCTCC CTCCTGGTCT CTCCTATCAG CTCCCCCCCC 
GACTCAGGCC CCGGCCCCCT CAGACCAGTA ATCAGCCATC CCTCCGGACT CTGCTACCGT 
CCTCCTGGCC CTGGGCCCCA CCACATCCCT CCTCCCTCAC CCTTCATGCC TCCCATGTAC 
CGACCAGCCA ACGGACACCC AGGCATGATG CCTCCCGGAC CCCCACCACC TAATGGACAC 
CCACCTGGCC CCATGATGCC ACCCGGACAG CGGCCTCCAC CTCCTGGTGC CTACGGCCCC 
CCACACCACC GGGGCCCTCC TCCACCTCAC TATGGACCAG TGCCTCCCCC ATTCGGTGTT 
CGCGGCGGCC CCCCTATGGC CCGACCCATG GGACCACCAC GTCCCTACAT GCACTACGGA 
CCACGTGATC ACTCCATCCC ACCCCAGCAC CTGCCCCCAG GAGCGGCCCC ATACCCTCCT 
CACAGTGGCC CCAGAGACTT CCCCGGGCAG CCCCCCATGC AGCAGGCCCC CCATGGGCAT 
GACTCTTCTG TGGGTCCCCA GGGGCAAGAC TACAGCTCCC AGCAGGCAGC AGCTACCCCC 
CAGGACTCAG TCAGCTCCTC CATGGGAGAG CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110529424 ( XM_021611584.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467259.1 Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOf04241
Clone ID Related Accession (Same CDS sequence)	XM_021611584.1
Accession Version	XM_021611584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	melanoma inhibitory activity protein 3-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGCAGACCG CTCCACCACA GGCTGAGGCC CAGGCCTCCA CAGAGAACCC AGAGGCAAAC 
TCAGGACCTC GGGGCCCTGG CTCCCTCCTG GTCTCTCCTA TCAGCTCCCC CCCCGACTCA 
GGCCCCGGCC CCCTCAGACC AGTAATCAGC CATCCCTCCG GACTCTGCTA CCGTCCTCCT 
GGCCCTGGGC CCCACCACAT CCCTCCTCCC TCACCCTTCA TGCCTCCCAT GTACCGACCA 
GCCAACGGAC ACCCAGGCAT GATGCCTCCC GGACCCCCAC CACCTAATGG ACACCCACCT 
GGCCCCATGA TGCCACCCGG ACAGCGGCCT CCACCTCCTG GTGCCTACGG CCCCCCACAC 
CACCGGGGCC CTCCTCCACC TCACTATGGA CCAGTGCCTC CCCCATTCGG TGTTCGCGGC 
GGCCCCCCTA TGGCCCGACC CATGGGACCA CCACGTCCCT ACATGCACTA CGGACCACGT 
GATCACTCCA TCCCACCCCA GCACCTGCCC CCAGGAGCGG CCCCATACCC TCCTCACAGT 
GGCCCCAGAG ACTTCCCCGG GCAGCCCCCC ATGCAGCAGG CCCCCCATGG GCATGACTCT 
TCTGTGGGTC CCCAGGGGCA AGACTACAGC TCCCAGCAGG CAGCAGCTAC CCCCCAGGAC 
TCAGTCAGCT CCTCCATGGG AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467259.1
CDS	19..5688
Translation	MLLCRGKASSDFTGPDCRFLSFKKGETVYVYYKLSGRRTDIWAGTVGSRFGYFHKDQLAINHIYTEKELEIPAQETDFVCFDTGHDKFDSYDIESLLVSSLLLTDQDKSVQGTTETLDRNKSAESTTVEVDEPPPEVTLLENNDVDSDVPEDIDKVLEEVLEEFPEDKDPGHFDPLESSLPQPEIETLDAKGVESNTVVEPTAERIKDDLQKYQLTTEDSVPDSVVEPEQGETKESLSEPLSKDDPELENAPDGFSEGRPVPQLKTTLGTTFDAVTSDDEETRKVTPYDEEDEGSEEFEYQQDDKSDYHLRETPLLAFSEEHSNLEHEDIDKVLEVLEDKDPGHFESDQTDEEESPPETAEKQDSKDKNLWSSLGDTVFNIVSGGERTAHVTGSEEEDDDDDDDEGEIVPEELPKIEEPKENIQLLANDPKQKPEVTEQPHEVIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPLADEVIPFEHTEEAIAENLTVNESPVPKHVSQTEMLSDFDSNTIDSEQTQAVEELPIQKEPKENIQLLANDPQQEPEVTEQPHEDIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPPADEVIPFEHTEEALAENLTVNESPFPKHVTQTELLSDFDSKTNVSEQKQAIELPIQKESRDFSQVENKLKLGGTELFRRFRGPNVPDPTKNTMVKERHEELPVDNEDLIDPENLEIEEELLEDENAALSSSKTEHTDKDKESRSEFGSEQSNYATETEVSSDELDVVQIEEAIDIEPEEHDIEDSESAGLDQTHTPSLEDKVPDPLSGQEPEYSDNVLRLTLLRNHFKEQDMKLFQKILGLQNLFRVEFLFSDLEQELNAALMSQTNTSEDIEKVLEAILEASETPILDEIERMLDARENADVQQETGELDVEATILDNFQELAFTLHQKYSMTSDSAPLAEGSQLHPDTDGDVSDAEEEKMFLHSVGDINEDNLTVTGEETKAPEEKPDHEGHNRPDLDMGLEEDVGHFNRNQDNQPEEIQRGPQAILENTLDMGLVDMEHPSSGSLESPPVSDFQEEEQSSSSIVSVLIFSGSLVTVVYEYLGIYAVMMVTCLPEEWKPGPDFYGVSWEPVLATAGAGFIGFLFLFWRSVLSVKRKSYLTTEKEMADRMKTFEQEKEEILLKVAELQQQGEELKEKKKLSEKSATSSLEKIQELKNLVQEMERQNECLDEEKNLLARSFDEERTKAAKHQDMMSEMDKTIEKLKRSRKKTQEALSKATILMEEAKLREDARNVQHHVLEKDIATLREENISLHHAANSWEEKHRELSEQIKVYQKSQKDLEDSLAQKDHNVEVLSDLLGDLEACDDLKGGVVANGEAANDKQTVIRNRIKQMMDVTRVQTTLSVVEEERDRFMTKLLNEEKARKELEEQYQKLEHAILLVKSDKNHLENQYKTLQQKNEIITEMYQQKENALQQKLTKEEFERRNNEDRLTEVDGKALEAEEEVKVCRQRIKEIQDELKQTEKSYKAQIIEQEQKSHENWVIARAAERALLDEKKESTNLRIKLTEMSSKLNELRRPLFKPTPGMAPMPLRRGDSYGPSPVSGGAPSPPLMMEGPGRPPSAPVGRRNEPYGPRPPSDPHGRYPPDHKHPVAARPDTIAPRTSSPSSLDSSQTAPPQAEAQASTENPEANSGPRGPGSLLVSPISSPPDSGPGPLRPVISHPSGLCYRPPGPGPHHIPPPSPFMPPMYRPANGHPGMMPPGPPPPNGHPPGPMMPPGQRPPPPGAYGPPHHRGPPPPHYGPVPPPFGVRGGPPMARPMGPPRPYMHYGPRDHSIPPQHLPPGAAPYPPHSGPRDFPGQPPMQQAPHGHDSSVGPQGQDYSSQQAAATPQDSVSSSMGEP

Target ORF information:

RefSeq Version	XM_021611584.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611584.1

ORF Insert Sequence:

ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGAGT TTCCAGAGGA TAAAGATCCA GGCCATTTTG ATCCTTTAGA GTCCTCTCTA 
CCTCAGCCTG AAATTGAAAC TCTTGACGCA AAAGGAGTTG AATCTAACAC AGTAGTTGAG 
CCCACAGCTG AGAGGATCAA AGATGACCTT CAGAAATATC AGTTGACGAC CGAGGACTCT 
GTGCCGGACA GTGTTGTTGA ACCAGAGCAA GGTGAGACTA AAGAAAGCCT TTCAGAACCT 
CTATCCAAAG ATGATCCTGA GTTGGAGAAT GCGCCAGATG GTTTTTCAGA AGGCAGACCT 
GTCCCTCAGT TGAAAACTAC ACTTGGAACA ACTTTTGATG CTGTCACCTC CGATGACGAG 
GAAACTAGGA AGGTGACTCC ATATGACGAG GAGGACGAGG GAAGTGAAGA GTTTGAATAT 
CAGCAGGATG ACAAAAGTGA TTATCATCTT AGGGAAACTC CATTGCTGGC TTTTTCTGAA 
GAACATTCTA ATTTAGAACA CGAAGACATT GATAAGGTTT TAGAGGTTTT AGAGGATAAA 
GATCCAGGCC ATTTTGAATC TGATCAGACA GATGAGGAAG AGAGTCCACC AGAGACAGCT 
GAAAAACAGG ACTCCAAGGA CAAGAACCTA TGGTCTTCAT TAGGTGATAC AGTTTTTAAC 
ATTGTCAGTG GTGGGGAAAG AACAGCTCAT GTTACAGGTT CAGAGGAAGA GGACGACGAC 
GACGATGACG ACGAAGGTGA GATTGTACCA GAGGAGCTTC CCAAAATTGA AGAACCCAAG 
GAGAATATAC AGCTGCTAGC CAACGACCCA AAGCAGAAGC CAGAGGTAAC AGAACAGCCA 
CATGAGGTCA TCTTTGAGGA ACCTATAGAC TCTGATTTCC TTGAGGCTGT CAAAATGCCT 
AATGTAGATT CCCAGACTTT GCAGTTGGAG GATGAACCTG AAGAAGGTGA CATTGAACCT 
TCAATACCAC TTGCTGATGA GGTGATTCCT TTTGAACATA CTGAGGAGGC TATAGCAGAG 
AATCTTACAG TAAATGAAAG CCCAGTCCCT AAACACGTCT CACAGACAGA AATGCTCTCA 
GACTTTGATA GTAATACTAT CGACTCAGAA CAGACGCAAG CTGTTGAAGA ACTCCCCATA 
CAAAAAGAAC CCAAGGAGAA TATACAGCTG CTAGCCAACG ACCCACAGCA GGAGCCAGAG 
GTAACAGAAC AGCCACACGA GGACATATTT GAGGAACCTA TAGACTCTGA TTTCCTTGAG 
GCTGTCAAAA TGCCTAATGT GGATTCCCAG ACTTTGCAGT TGGAGGATGA ACCTGAAGAA 
GGTGACATTG AACCTTCAAT ACCGCCTGCT GATGAGGTGA TTCCTTTTGA ACATACTGAG 
GAGGCTTTAG CAGAGAATCT TACAGTAAAT GAAAGCCCAT TCCCTAAACA CGTCACACAG 
ACAGAACTGC TCTCAGACTT TGATAGTAAA ACTAACGTAT CAGAGCAGAA ACAAGCTATT 
GAACTCCCTA TACAAAAAGA GTCAAGAGAT TTCTCACAAG TAGAGAATAA ATTGAAACTG 
GGTGGTACAG AGCTTTTCAG ACGATTTAGA GGACCTAACG TCCCAGATCC AACTAAAAAC 
ACAATGGTTA AAGAGAGGCA TGAAGAACTC CCTGTAGACA ATGAGGATTT GATAGACCCA 
GAGAACTTGG AGATTGAAGA AGAATTGCTA GAGGATGAAA ATGCAGCATT GTCTTCATCC 
AAAACCGAGC ACACTGATAA GGACAAAGAA AGTAGATCGG AATTTGGGTC AGAGCAATCC 
AATTATGCTA CAGAAACCGA GGTATCTAGT GATGAGTTGG ATGTTGTCCA AATTGAGGAG 
GCTATTGACA TAGAACCTGA GGAGCATGAC ATAGAAGATT CAGAAAGCGC AGGGTTGGAT 
CAAACACATA CACCCTCACT TGAAGACAAA GTTCCGGATC CCCTTTCAGG CCAAGAGCCA 
GAATACAGTG ATAATGTGTT GAGGCTGACG CTACTGCGCA ACCACTTCAA GGAACAGGAT 
ATGAAGCTCT TCCAGAAGAT TCTGGGTCTT CAGAACCTCT TCAGGGTGGA GTTCTTGTTC 
TCTGACCTGG AGCAGGAGCT GAATGCTGCC CTGATGTCAC AGACAAACAC CAGTGAGGAC 
ATTGAAAAGG TGCTGGAGGC CATTTTGGAG GCCTCTGAGA CCCCAATCCT GGATGAGATT 
GAGAGGATGC TGGATGCCCG GGAGAATGCT GACGTACAGC AGGAGACTGG TGAGCTTGAT 
GTGGAAGCCA CCATCTTGGA CAACTTCCAA GAGTTGGCGT TCACCCTGCA TCAGAAGTAC 
TCAATGACCA GCGACAGTGC TCCCTTGGCA GAGGGCAGTC AACTACACCC TGACACCGAT 
GGGGACGTGT CTGATGCAGA GGAAGAAAAG ATGTTCCTGC ACTCTGTGGG GGACATAAAC 
GAGGACAACC TCACGGTGAC GGGTGAAGAG ACTAAAGCAC CTGAGGAGAA GCCTGATCAT 
GAAGGCCACA ACAGACCAGA TCTGGATATG GGTCTTGAGG AGGATGTGGG GCATTTTAAC 
AGAAACCAAG ACAATCAACC AGAAGAAATC CAGAGGGGCC CTCAAGCTAT TTTGGAAAAT 
ACCTTGGACA TGGGACTTGT TGACATGGAG CACCCTTCCT CAGGCTCTTT GGAGTCGCCC 
CCTGTTTCTG ATTTCCAGGA AGAGGAGCAG AGTAGTTCAT CAATCGTATC GGTGTTAATT 
TTCTCTGGTA GTCTAGTCAC CGTGGTTTAT GAGTACCTTG GAATATATGC TGTTATGATG 
GTCACCTGCC TGCCAGAGGA ATGGAAGCCA GGTCCAGACT TTTACGGCGT GTCCTGGGAA 
CCCGTGCTGG CCACTGCAGG TGCCGGGTTC ATCGGCTTCC TCTTCCTCTT TTGGAGGAGT 
GTTCTATCTG TCAAAAGGAA GTCCTATCTA ACTACTGAAA AAGAGATGGC AGACAGGATG 
AAAACATTTG AACAAGAGAA GGAGGAGATA CTCCTGAAGG TTGCTGAACT GCAACAACAG 
GGTGAAGAAC TCAAAGAAAA GAAAAAGCTG TCTGAGAAAT CTGCCACCTC ATCTCTGGAA 
AAGATCCAAG AATTAAAAAA TCTTGTGCAA GAGATGGAGA GACAAAATGA GTGCCTGGAC 
GAGGAAAAGA ACTTGCTCGC CAGATCCTTT GACGAAGAGC GGACAAAGGC GGCGAAACAT 
CAAGATATGA TGTCAGAAAT GGACAAAACT ATTGAGAAGT TGAAGCGGAG CAGAAAGAAG 
ACTCAGGAGG CTCTCTCAAA GGCTACCATT CTGATGGAAG AAGCCAAGCT CCGTGAAGAT 
GCACGGAATG TCCAGCACCA TGTTCTGGAG AAGGATATCG CCACCCTGAG AGAGGAGAAC 
ATCTCGCTGC ACCACGCTGC CAATTCGTGG GAGGAGAAGC ACAGGGAGTT GAGCGAGCAG 
ATCAAAGTCT ACCAGAAGTC CCAGAAAGAT CTGGAGGACT CCCTTGCTCA GAAGGACCAC 
AACGTTGAGG TTTTGTCTGA CCTCCTGGGA GACCTCGAGG CCTGCGACGA CCTGAAAGGA 
GGCGTCGTGG CTAACGGGGA AGCCGCTAAC GACAAGCAGA CCGTCATCCG AAACCGCATT 
AAGCAAATGA TGGATGTCAC TCGGGTCCAG ACCACTCTGT CTGTTGTGGA GGAAGAGCGC 
GATCGCTTCA TGACCAAGCT GCTGAATGAA GAGAAAGCCA GGAAAGAGCT GGAAGAACAA 
TACCAGAAGC TGGAGCATGC CATCTTGTTG GTGAAAAGTG ATAAGAACCA CCTGGAGAAC 
CAGTACAAGA CCCTGCAGCA GAAGAACGAG ATCATCACAG AGATGTACCA GCAGAAGGAG 
AACGCCTTGC AGCAGAAGCT AACCAAGGAG GAGTTTGAGC GTCGCAACAA CGAGGATAGG 
CTGACTGAGG TGGACGGCAA GGCCCTGGAG GCCGAGGAGG AGGTCAAGGT GTGCAGGCAG 
CGTATCAAGG AGATCCAGGA TGAGCTGAAA CAGACTGAAA AGTCCTACAA AGCCCAGATC 
ATTGAGCAGG AGCAGAAATC CCATGAGAAC TGGGTTATTG CACGCGCCGC AGAGCGAGCT 
CTGTTGGACG AGAAAAAGGA ATCAACCAAC CTCCGTATTA AACTGACGGA GATGTCCAGC 
AAGCTGAATG AGCTCCGTAG GCCTCTATTC AAACCCACCC CTGGGATGGC TCCCATGCCT 
CTCCGACGAG GTGACTCGTA CGGGCCCTCG CCCGTGAGCG GAGGTGCCCC GTCCCCCCCT 
CTCATGATGG AGGGTCCTGG GCGACCCCCC TCTGCCCCTG TGGGGAGACG GAACGAGCCC 
TACGGACCAC GACCTCCATC TGACCCTCAC GGTCGTTACC CCCCTGACCA CAAACACCCA 
GTGGCTGCCA GACCTGACAC CATTGCACCA CGGACATCCT CTCCAAGCAG TCTGGACAGC 
TCGCAGACCG CTCCACCACA GGCTGAGGCC CAGGCCTCCA CAGAGAACCC AGAGGCAAAC 
TCAGGACCTC GGGGCCCTGG CTCCCTCCTG GTCTCTCCTA TCAGCTCCCC CCCCGACTCA 
GGCCCCGGCC CCCTCAGACC AGTAATCAGC CATCCCTCCG GACTCTGCTA CCGTCCTCCT 
GGCCCTGGGC CCCACCACAT CCCTCCTCCC TCACCCTTCA TGCCTCCCAT GTACCGACCA 
GCCAACGGAC ACCCAGGCAT GATGCCTCCC GGACCCCCAC CACCTAATGG ACACCCACCT 
GGCCCCATGA TGCCACCCGG ACAGCGGCCT CCACCTCCTG GTGCCTACGG CCCCCCACAC 
CACCGGGGCC CTCCTCCACC TCACTATGGA CCAGTGCCTC CCCCATTCGG TGTTCGCGGC 
GGCCCCCCTA TGGCCCGACC CATGGGACCA CCACGTCCCT ACATGCACTA CGGACCACGT 
GATCACTCCA TCCCACCCCA GCACCTGCCC CCAGGAGCGG CCCCATACCC TCCTCACAGT 
GGCCCCAGAG ACTTCCCCGG GCAGCCCCCC ATGCAGCAGG CCCCCCATGG GCATGACTCT 
TCTGTGGGTC CCCAGGGGCA AGACTACAGC TCCCAGCAGG CAGCAGCTAC CCCCCAGGAC 
TCAGTCAGCT CCTCCATGGG AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110529424 ( XM_021611585.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467260.1 Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X5, mRNA.

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CloneID	OOf04242
Clone ID Related Accession (Same CDS sequence)	XM_021611585.1
Accession Version	XM_021611585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	melanoma inhibitory activity protein 3-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGATA AAGATCCAGG CCATTTTGAA TCTGATCAGA CAGATGAGGA AGAGAGTCCA 
CCAGAGACAG CTGAAAAACA GGACTCCAAG GACAAGAACC TATGGTCTTC ATTAGGTGAT 
ACAGTTTTTA ACATTGTCAG TGGTGGGGAA AGAACAGCTC ATGTTACAGG TTCAGAGGAA 
GAGGACGACG ACGACGATGA CGACGAAGGT GAGATTGTAC CAGAGGAGCT TCCCAAAATT 
GAAGAACCCA AGGAGAATAT ACAGCTGCTA GCCAACGACC CAAAGCAGAA GCCAGAGGTA 
ACAGAACAGC CACATGAGGT CATCTTTGAG GAACCTATAG ACTCTGATTT CCTTGAGGCT 
GTCAAAATGC CTAATGTAGA TTCCCAGACT TTGCAGTTGG AGGATGAACC TGAAGAAGGT 
GACATTGAAC CTTCAATACC ACTTGCTGAT GAGGTGATTC CTTTTGAACA TACTGAGGAG 
GCTATAGCAG AGAATCTTAC AGTAAATGAA AGCCCAGTCC CTAAACACGT CTCACAGACA 
GAAATGCTCT CAGACTTTGA TAGTAATACT ATCGACTCAG AACAGACGCA AGCTGTTGAA 
GAACTCCCCA TACAAAAAGA ACCCAAGGAG AATATACAGC TGCTAGCCAA CGACCCACAG 
CAGGAGCCAG AGGTAACAGA ACAGCCACAC GAGGACATAT TTGAGGAACC TATAGACTCT 
GATTTCCTTG AGGCTGTCAA AATGCCTAAT GTGGATTCCC AGACTTTGCA GTTGGAGGAT 
GAACCTGAAG AAGGTGACAT TGAACCTTCA ATACCGCCTG CTGATGAGGT GATTCCTTTT 
GAACATACTG AGGAGGCTTT AGCAGAGAAT CTTACAGTAA ATGAAAGCCC ATTCCCTAAA 
CACGTCACAC AGACAGAACT GCTCTCAGAC TTTGATAGTA AAACTAACGT ATCAGAGCAG 
AAACAAGCTA TTGAACTCCC TATACAAAAA GAGTCAAGAG ATTTCTCACA AGTAGAGAAT 
AAATTGAAAC TGGGTGGTAC AGAGCTTTTC AGACGATTTA GAGGACCTAA CGTCCCAGAT 
CCAACTAAAA ACACAATGGT TAAAGAGAGG CATGAAGAAC TCCCTGTAGA CAATGAGGAT 
TTGATAGACC CAGAGAACTT GGAGATTGAA GAAGAATTGC TAGAGGATGA AAATGCAGCA 
TTGTCTTCAT CCAAAACCGA GCACACTGAT AAGGACAAAG AAAGTAGATC GGAATTTGGG 
TCAGAGCAAT CCAATTATGC TACAGAAACC GAGGTATCTA GTGATGAGTT GGATGTTGTC 
CAAATTGAGG AGGCTATTGA CATAGAACCT GAGGAGCATG ACATAGAAGA TTCAGAAAGC 
GCAGGGTTGG ATCAAACACA TACACCCTCA CTTGAAGACA AAGTTCCGGA TCCCCTTTCA 
GGCCAAGAGC CAGAATACAG TGATAATGTG TTGAGGCTGA CGCTACTGCG CAACCACTTC 
AAGGAACAGG ATATGAAGCT CTTCCAGAAG ATTCTGGGTC TTCAGAACCT CTTCAGGGTG 
GAGTTCTTGT TCTCTGACCT GGAGCAGGAG CTGAATGCTG CCCTGATGTC ACAGACAAAC 
ACCAGTGAGG ACATTGAAAA GGTGCTGGAG GCCATTTTGG AGGCCTCTGA GACCCCAATC 
CTGGATGAGA TTGAGAGGAT GCTGGATGCC CGGGAGAATG CTGACGTACA GCAGGAGACT 
GGTGAGCTTG ATGTGGAAGC CACCATCTTG GACAACTTCC AAGAGTTGGC GTTCACCCTG 
CATCAGAAGT ACTCAATGAC CAGCGACAGT GCTCCCTTGG CAGAGGGCAG TCAACTACAC 
CCTGACACCG ATGGGGACGT GTCTGATGCA GAGGAAGAAA AGATGTTCCT GCACTCTGTG 
GGGGACATAA ACGAGGACAA CCTCACGGTG ACGGGTGAAG AGACTAAAGC ACCTGAGGAG 
AAGCCTGATC ATGAAGGCCA CAACAGACCA GATCTGGATA TGGGTCTTGA GGAGGATGTG 
GGGCATTTTA ACAGAAACCA AGACAATCAA CCAGAAGAAA TCCAGAGGGG CCCTCAAGCT 
ATTTTGGAAA ATACCTTGGA CATGGGACTT GTTGACATGG AGCACCCTTC CTCAGGCTCT 
TTGGAGTCGC CCCCTGTTTC TGATTTCCAG GAAGAGGAGC AGAGTAGTTC ATCAATCGTA 
TCGGTGTTAA TTTTCTCTGG TAGTCTAGTC ACCGTGGTTT ATGAGTACCT TGGAATATAT 
GCTGTTATGA TGGTCACCTG CCTGCCAGAG GAATGGAAGC CAGGTCCAGA CTTTTACGGC 
GTGTCCTGGG AACCCGTGCT GGCCACTGCA GGTGCCGGGT TCATCGGCTT CCTCTTCCTC 
TTTTGGAGGA GTGTTCTATC TGTCAAAAGG AAGTCCTATC TAACTACTGA AAAAGAGATG 
GCAGACAGGA TGAAAACATT TGAACAAGAG AAGGAGGAGA TACTCCTGAA GGTTGCTGAA 
CTGCAACAAC AGGGTGAAGA ACTCAAAGAA AAGAAAAAGC TGTCTGAGAA ATCTGCCACC 
TCATCTCTGG AAAAGATCCA AGAATTAAAA AATCTTGTGC AAGAGATGGA GAGACAAAAT 
GAGTGCCTGG ACGAGGAAAA GAACTTGCTC GCCAGATCCT TTGACGAAGA GCGGACAAAG 
GCGGCGAAAC ATCAAGATAT GATGTCAGAA ATGGACAAAA CTATTGAGAA GTTGAAGCGG 
AGCAGAAAGA AGACTCAGGA GGCTCTCTCA AAGGCTACCA TTCTGATGGA AGAAGCCAAG 
CTCCGTGAAG ATGCACGGAA TGTCCAGCAC CATGTTCTGG AGAAGGATAT CGCCACCCTG 
AGAGAGGAGA ACATCTCGCT GCACCACGCT GCCAATTCGT GGGAGGAGAA GCACAGGGAG 
TTGAGCGAGC AGATCAAAGT CTACCAGAAG TCCCAGAAAG ATCTGGAGGA CTCCCTTGCT 
CAGAAGGACC ACAACGTTGA GGTTTTGTCT GACCTCCTGG GAGACCTCGA GGCCTGCGAC 
GACCTGAAAG GAGGCGTCGT GGCTAACGGG GAAGCCGCTA ACGACAAGCA GACCGTCATC 
CGAAACCGCA TTAAGCAAAT GATGGATGTC ACTCGGGTCC AGACCACTCT GTCTGTTGTG 
GAGGAAGAGC GCGATCGCTT CATGACCAAG CTGCTGAATG AAGAGAAAGC CAGGAAAGAG 
CTGGAAGAAC AATACCAGAA GCTGGAGCAT GCCATCTTGT TGGTGAAAAG TGATAAGAAC 
CACCTGGAGA ACCAGTACAA GACCCTGCAG CAGAAGAACG AGATCATCAC AGAGATGTAC 
CAGCAGAAGG AGAACGCCTT GCAGCAGAAG CTAACCAAGG AGGAGTTTGA GCGTCGCAAC 
AACGAGGATA GGCTGACTGA GGTGGACGGC AAGGCCCTGG AGGCCGAGGA GGAGGTCAAG 
GTGTGCAGGC AGCGTATCAA GGAGATCCAG GATGAGCTGA AACAGACTGA AAAGTCCTAC 
AAAGCCCAGA TCATTGAGCA GGAGCAGAAA TCCCATGAGA ACTGGGTTAT TGCACGCGCC 
GCAGAGCGAG CTCTGTTGGA CGAGAAAAAG GAATCAACCA ACCTCCGTAT TAAACTGACG 
GAGATGTCCA GCAAGCTGAA TGAGCTCCGT AGGCCTCTAT TCAAACCCAC CCCTGGGATG 
GCTCCCATGC CTCTCCGACG AGGTGACTCG TACGGGCCCT CGCCCGTGAG CGGAGGTGCC 
CCGTCCCCCC CTCTCATGAT GGAGGGTCCT GGGCGACCCC CCTCTGCCCC TGTGGGGAGA 
CGGAACGAGC CCTACGGACC ACGACCTCCA TCTGACCCTC ACGGTCGTTA CCCCCCTGAC 
CACAAACACC CAGTGGCTGC CAGACCTGAC ACCATTGCAC CACGGACATC CTCTCCAAGC 
AGTCTGGACA GCTCGCAGAC CGCTCCACCA CAGGCTGAGG CCCAGGCCTC CACAGAGAAC 
CCAGAGGCAA ACTCAGGACC TCGGGGCCCT GGCTCCCTCC TGGTCTCTCC TATCAGCTCC 
CCCCCCGACT CAGGCCCCGG CCCCCTCAGA CCAGTAATCA GCCATCCCTC CGGACTCTGC 
TACCGTCCTC CTGGCCCTGG GCCCCACCAC ATCCCTCCTC CCTCACCCTT CATGCCTCCC 
ATGTACCGAC CAGCCAACGG ACACCCAGGC ATGATGCCTC CCGGACCCCC ACCACCTAAT 
GGACACCCAC CTGGCCCCAT GATGCCACCC GGACAGCGGC CTCCACCTCC TGGTGCCTAC 
GGCCCCCCAC ACCACCGGGG CCCTCCTCCA CCTCACTATG GACCAGTGCC TCCCCCATTC 
GGTGTTCGCG GCGGCCCCCC TATGGCCCGA CCCATGGGAC CACCACGTCC CTACATGCAC 
TACGGACCAC GTGATCACTC CATCCCACCC CAGCACCTGC CCCCAGGAGC GGCCCCATAC 
CCTCCTCACA GTGGCCCCAG AGACTTCCCC GGGCAGCCCC CCATGCAGCA GGCCCCCCAT 
GGGCATGACT CTTCTGTGGG TCCCCAGGGG CAAGACTACA GCTCCCAGCA GGCAGCAGCT 
ACCCCCCAGG ACTCAGTCAG CTCCTCCATG GGAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467260.1
CDS	375..5636
Translation	MAVNVSHVYILLLFIINFISNAATERRFSDFKRCADEECSMLLCRGKASSDFTGPDCRFLSFKKGETVYVYYKLSGRRTDIWAGTVGSRFGYFHKDQLAINHIYTEKELEIPAQETDFVCFDTGHDKFDSYDIESLLVSSLLLTDQDKSVQGTTETLDRNKSAESTTVEVDEPPPEVTLLENNDVDSDVPEDIDKVLEEVLEDKDPGHFESDQTDEEESPPETAEKQDSKDKNLWSSLGDTVFNIVSGGERTAHVTGSEEEDDDDDDDEGEIVPEELPKIEEPKENIQLLANDPKQKPEVTEQPHEVIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPLADEVIPFEHTEEAIAENLTVNESPVPKHVSQTEMLSDFDSNTIDSEQTQAVEELPIQKEPKENIQLLANDPQQEPEVTEQPHEDIFEEPIDSDFLEAVKMPNVDSQTLQLEDEPEEGDIEPSIPPADEVIPFEHTEEALAENLTVNESPFPKHVTQTELLSDFDSKTNVSEQKQAIELPIQKESRDFSQVENKLKLGGTELFRRFRGPNVPDPTKNTMVKERHEELPVDNEDLIDPENLEIEEELLEDENAALSSSKTEHTDKDKESRSEFGSEQSNYATETEVSSDELDVVQIEEAIDIEPEEHDIEDSESAGLDQTHTPSLEDKVPDPLSGQEPEYSDNVLRLTLLRNHFKEQDMKLFQKILGLQNLFRVEFLFSDLEQELNAALMSQTNTSEDIEKVLEAILEASETPILDEIERMLDARENADVQQETGELDVEATILDNFQELAFTLHQKYSMTSDSAPLAEGSQLHPDTDGDVSDAEEEKMFLHSVGDINEDNLTVTGEETKAPEEKPDHEGHNRPDLDMGLEEDVGHFNRNQDNQPEEIQRGPQAILENTLDMGLVDMEHPSSGSLESPPVSDFQEEEQSSSSIVSVLIFSGSLVTVVYEYLGIYAVMMVTCLPEEWKPGPDFYGVSWEPVLATAGAGFIGFLFLFWRSVLSVKRKSYLTTEKEMADRMKTFEQEKEEILLKVAELQQQGEELKEKKKLSEKSATSSLEKIQELKNLVQEMERQNECLDEEKNLLARSFDEERTKAAKHQDMMSEMDKTIEKLKRSRKKTQEALSKATILMEEAKLREDARNVQHHVLEKDIATLREENISLHHAANSWEEKHRELSEQIKVYQKSQKDLEDSLAQKDHNVEVLSDLLGDLEACDDLKGGVVANGEAANDKQTVIRNRIKQMMDVTRVQTTLSVVEEERDRFMTKLLNEEKARKELEEQYQKLEHAILLVKSDKNHLENQYKTLQQKNEIITEMYQQKENALQQKLTKEEFERRNNEDRLTEVDGKALEAEEEVKVCRQRIKEIQDELKQTEKSYKAQIIEQEQKSHENWVIARAAERALLDEKKESTNLRIKLTEMSSKLNELRRPLFKPTPGMAPMPLRRGDSYGPSPVSGGAPSPPLMMEGPGRPPSAPVGRRNEPYGPRPPSDPHGRYPPDHKHPVAARPDTIAPRTSSPSSLDSSQTAPPQAEAQASTENPEANSGPRGPGSLLVSPISSPPDSGPGPLRPVISHPSGLCYRPPGPGPHHIPPPSPFMPPMYRPANGHPGMMPPGPPPPNGHPPGPMMPPGQRPPPPGAYGPPHHRGPPPPHYGPVPPPFGVRGGPPMARPMGPPRPYMHYGPRDHSIPPQHLPPGAAPYPPHSGPRDFPGQPPMQQAPHGHDSSVGPQGQDYSSQQAAATPQDSVSSSMGEP

Target ORF information:

RefSeq Version	XM_021611585.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss melanoma inhibitory activity protein 3-like (LOC110529424), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611585.1

ORF Insert Sequence:

ATGGCTGTAA ATGTATCTCA TGTATACATT TTACTTTTAT TTATTATTAA TTTCATATCC 
AATGCCGCCA CCGAAAGGAG ATTCTCCGAC TTCAAACGAT GTGCTGACGA AGAATGCAGT 
ATGCTCCTGT GCCGGGGGAA AGCATCAAGT GATTTCACTG GACCAGACTG TCGGTTCCTG 
TCTTTTAAAA AAGGAGAGAC GGTATATGTC TACTATAAAC TCTCAGGCAG AAGGACAGAT 
ATATGGGCGG GAACTGTTGG TAGCCGCTTT GGTTACTTCC ATAAGGACCA ACTCGCAATC 
AATCACATAT ACACTGAAAA AGAATTGGAG ATTCCTGCTC AGGAAACCGA CTTTGTTTGT 
TTTGACACCG GACACGATAA GTTTGACAGT TATGACATTG AGTCACTGTT AGTCTCCTCC 
TTATTGTTAA CGGACCAAGA CAAGTCTGTA CAAGGAACCA CAGAGACTTT AGACCGAAAC 
AAAAGTGCAG AGAGCACCAC AGTGGAAGTG GATGAGCCTC CACCTGAAGT GACTCTGTTA 
GAAAACAATG ATGTTGATAG TGATGTTCCT GAGGACATTG ATAAGGTTTT AGAAGAGGTT 
TTAGAGGATA AAGATCCAGG CCATTTTGAA TCTGATCAGA CAGATGAGGA AGAGAGTCCA 
CCAGAGACAG CTGAAAAACA GGACTCCAAG GACAAGAACC TATGGTCTTC ATTAGGTGAT 
ACAGTTTTTA ACATTGTCAG TGGTGGGGAA AGAACAGCTC ATGTTACAGG TTCAGAGGAA 
GAGGACGACG ACGACGATGA CGACGAAGGT GAGATTGTAC CAGAGGAGCT TCCCAAAATT 
GAAGAACCCA AGGAGAATAT ACAGCTGCTA GCCAACGACC CAAAGCAGAA GCCAGAGGTA 
ACAGAACAGC CACATGAGGT CATCTTTGAG GAACCTATAG ACTCTGATTT CCTTGAGGCT 
GTCAAAATGC CTAATGTAGA TTCCCAGACT TTGCAGTTGG AGGATGAACC TGAAGAAGGT 
GACATTGAAC CTTCAATACC ACTTGCTGAT GAGGTGATTC CTTTTGAACA TACTGAGGAG 
GCTATAGCAG AGAATCTTAC AGTAAATGAA AGCCCAGTCC CTAAACACGT CTCACAGACA 
GAAATGCTCT CAGACTTTGA TAGTAATACT ATCGACTCAG AACAGACGCA AGCTGTTGAA 
GAACTCCCCA TACAAAAAGA ACCCAAGGAG AATATACAGC TGCTAGCCAA CGACCCACAG 
CAGGAGCCAG AGGTAACAGA ACAGCCACAC GAGGACATAT TTGAGGAACC TATAGACTCT 
GATTTCCTTG AGGCTGTCAA AATGCCTAAT GTGGATTCCC AGACTTTGCA GTTGGAGGAT 
GAACCTGAAG AAGGTGACAT TGAACCTTCA ATACCGCCTG CTGATGAGGT GATTCCTTTT 
GAACATACTG AGGAGGCTTT AGCAGAGAAT CTTACAGTAA ATGAAAGCCC ATTCCCTAAA 
CACGTCACAC AGACAGAACT GCTCTCAGAC TTTGATAGTA AAACTAACGT ATCAGAGCAG 
AAACAAGCTA TTGAACTCCC TATACAAAAA GAGTCAAGAG ATTTCTCACA AGTAGAGAAT 
AAATTGAAAC TGGGTGGTAC AGAGCTTTTC AGACGATTTA GAGGACCTAA CGTCCCAGAT 
CCAACTAAAA ACACAATGGT TAAAGAGAGG CATGAAGAAC TCCCTGTAGA CAATGAGGAT 
TTGATAGACC CAGAGAACTT GGAGATTGAA GAAGAATTGC TAGAGGATGA AAATGCAGCA 
TTGTCTTCAT CCAAAACCGA GCACACTGAT AAGGACAAAG AAAGTAGATC GGAATTTGGG 
TCAGAGCAAT CCAATTATGC TACAGAAACC GAGGTATCTA GTGATGAGTT GGATGTTGTC 
CAAATTGAGG AGGCTATTGA CATAGAACCT GAGGAGCATG ACATAGAAGA TTCAGAAAGC 
GCAGGGTTGG ATCAAACACA TACACCCTCA CTTGAAGACA AAGTTCCGGA TCCCCTTTCA 
GGCCAAGAGC CAGAATACAG TGATAATGTG TTGAGGCTGA CGCTACTGCG CAACCACTTC 
AAGGAACAGG ATATGAAGCT CTTCCAGAAG ATTCTGGGTC TTCAGAACCT CTTCAGGGTG 
GAGTTCTTGT TCTCTGACCT GGAGCAGGAG CTGAATGCTG CCCTGATGTC ACAGACAAAC 
ACCAGTGAGG ACATTGAAAA GGTGCTGGAG GCCATTTTGG AGGCCTCTGA GACCCCAATC 
CTGGATGAGA TTGAGAGGAT GCTGGATGCC CGGGAGAATG CTGACGTACA GCAGGAGACT 
GGTGAGCTTG ATGTGGAAGC CACCATCTTG GACAACTTCC AAGAGTTGGC GTTCACCCTG 
CATCAGAAGT ACTCAATGAC CAGCGACAGT GCTCCCTTGG CAGAGGGCAG TCAACTACAC 
CCTGACACCG ATGGGGACGT GTCTGATGCA GAGGAAGAAA AGATGTTCCT GCACTCTGTG 
GGGGACATAA ACGAGGACAA CCTCACGGTG ACGGGTGAAG AGACTAAAGC ACCTGAGGAG 
AAGCCTGATC ATGAAGGCCA CAACAGACCA GATCTGGATA TGGGTCTTGA GGAGGATGTG 
GGGCATTTTA ACAGAAACCA AGACAATCAA CCAGAAGAAA TCCAGAGGGG CCCTCAAGCT 
ATTTTGGAAA ATACCTTGGA CATGGGACTT GTTGACATGG AGCACCCTTC CTCAGGCTCT 
TTGGAGTCGC CCCCTGTTTC TGATTTCCAG GAAGAGGAGC AGAGTAGTTC ATCAATCGTA 
TCGGTGTTAA TTTTCTCTGG TAGTCTAGTC ACCGTGGTTT ATGAGTACCT TGGAATATAT 
GCTGTTATGA TGGTCACCTG CCTGCCAGAG GAATGGAAGC CAGGTCCAGA CTTTTACGGC 
GTGTCCTGGG AACCCGTGCT GGCCACTGCA GGTGCCGGGT TCATCGGCTT CCTCTTCCTC 
TTTTGGAGGA GTGTTCTATC TGTCAAAAGG AAGTCCTATC TAACTACTGA AAAAGAGATG 
GCAGACAGGA TGAAAACATT TGAACAAGAG AAGGAGGAGA TACTCCTGAA GGTTGCTGAA 
CTGCAACAAC AGGGTGAAGA ACTCAAAGAA AAGAAAAAGC TGTCTGAGAA ATCTGCCACC 
TCATCTCTGG AAAAGATCCA AGAATTAAAA AATCTTGTGC AAGAGATGGA GAGACAAAAT 
GAGTGCCTGG ACGAGGAAAA GAACTTGCTC GCCAGATCCT TTGACGAAGA GCGGACAAAG 
GCGGCGAAAC ATCAAGATAT GATGTCAGAA ATGGACAAAA CTATTGAGAA GTTGAAGCGG 
AGCAGAAAGA AGACTCAGGA GGCTCTCTCA AAGGCTACCA TTCTGATGGA AGAAGCCAAG 
CTCCGTGAAG ATGCACGGAA TGTCCAGCAC CATGTTCTGG AGAAGGATAT CGCCACCCTG 
AGAGAGGAGA ACATCTCGCT GCACCACGCT GCCAATTCGT GGGAGGAGAA GCACAGGGAG 
TTGAGCGAGC AGATCAAAGT CTACCAGAAG TCCCAGAAAG ATCTGGAGGA CTCCCTTGCT 
CAGAAGGACC ACAACGTTGA GGTTTTGTCT GACCTCCTGG GAGACCTCGA GGCCTGCGAC 
GACCTGAAAG GAGGCGTCGT GGCTAACGGG GAAGCCGCTA ACGACAAGCA GACCGTCATC 
CGAAACCGCA TTAAGCAAAT GATGGATGTC ACTCGGGTCC AGACCACTCT GTCTGTTGTG 
GAGGAAGAGC GCGATCGCTT CATGACCAAG CTGCTGAATG AAGAGAAAGC CAGGAAAGAG 
CTGGAAGAAC AATACCAGAA GCTGGAGCAT GCCATCTTGT TGGTGAAAAG TGATAAGAAC 
CACCTGGAGA ACCAGTACAA GACCCTGCAG CAGAAGAACG AGATCATCAC AGAGATGTAC 
CAGCAGAAGG AGAACGCCTT GCAGCAGAAG CTAACCAAGG AGGAGTTTGA GCGTCGCAAC 
AACGAGGATA GGCTGACTGA GGTGGACGGC AAGGCCCTGG AGGCCGAGGA GGAGGTCAAG 
GTGTGCAGGC AGCGTATCAA GGAGATCCAG GATGAGCTGA AACAGACTGA AAAGTCCTAC 
AAAGCCCAGA TCATTGAGCA GGAGCAGAAA TCCCATGAGA ACTGGGTTAT TGCACGCGCC 
GCAGAGCGAG CTCTGTTGGA CGAGAAAAAG GAATCAACCA ACCTCCGTAT TAAACTGACG 
GAGATGTCCA GCAAGCTGAA TGAGCTCCGT AGGCCTCTAT TCAAACCCAC CCCTGGGATG 
GCTCCCATGC CTCTCCGACG AGGTGACTCG TACGGGCCCT CGCCCGTGAG CGGAGGTGCC 
CCGTCCCCCC CTCTCATGAT GGAGGGTCCT GGGCGACCCC CCTCTGCCCC TGTGGGGAGA 
CGGAACGAGC CCTACGGACC ACGACCTCCA TCTGACCCTC ACGGTCGTTA CCCCCCTGAC 
CACAAACACC CAGTGGCTGC CAGACCTGAC ACCATTGCAC CACGGACATC CTCTCCAAGC 
AGTCTGGACA GCTCGCAGAC CGCTCCACCA CAGGCTGAGG CCCAGGCCTC CACAGAGAAC 
CCAGAGGCAA ACTCAGGACC TCGGGGCCCT GGCTCCCTCC TGGTCTCTCC TATCAGCTCC 
CCCCCCGACT CAGGCCCCGG CCCCCTCAGA CCAGTAATCA GCCATCCCTC CGGACTCTGC 
TACCGTCCTC CTGGCCCTGG GCCCCACCAC ATCCCTCCTC CCTCACCCTT CATGCCTCCC 
ATGTACCGAC CAGCCAACGG ACACCCAGGC ATGATGCCTC CCGGACCCCC ACCACCTAAT 
GGACACCCAC CTGGCCCCAT GATGCCACCC GGACAGCGGC CTCCACCTCC TGGTGCCTAC 
GGCCCCCCAC ACCACCGGGG CCCTCCTCCA CCTCACTATG GACCAGTGCC TCCCCCATTC 
GGTGTTCGCG GCGGCCCCCC TATGGCCCGA CCCATGGGAC CACCACGTCC CTACATGCAC 
TACGGACCAC GTGATCACTC CATCCCACCC CAGCACCTGC CCCCAGGAGC GGCCCCATAC 
CCTCCTCACA GTGGCCCCAG AGACTTCCCC GGGCAGCCCC CCATGCAGCA GGCCCCCCAT 
GGGCATGACT CTTCTGTGGG TCCCCAGGGG CAAGACTACA GCTCCCAGCA GGCAGCAGCT 
ACCCCCCAGG ACTCAGTCAG CTCCTCCATG GGAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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LOC110529424 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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