Adgrf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adgrf1
Entrez Gene ID	77596
Full Name	adhesion G protein-coupled receptor F1
Synonyms	5031409J19Rik,Gpr110,KPG_009
General protein information	Preferred Names adhesion G protein-coupled receptor F1 Names adhesion G-protein coupled receptor F1 G protein coupled receptor Gpr110 G-protein coupled receptor 110 internal gene name of KIRIN laboratory:M5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525120.4	XP_006525183.1	adhesion G-protein coupled receptor F1 isoform X1
NM_133776.2	NP_598537.2	adhesion G-protein coupled receptor F1 precursor
XM_006525121.4	XP_006525184.1	adhesion G-protein coupled receptor F1 isoform X2
XM_006525121.3	XP_006525184.1	adhesion G-protein coupled receptor F1 isoform X2
XM_006525120.3	XP_006525183.1	adhesion G-protein coupled receptor F1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	GPR110	XP_001103695.2
Pan troglodytes (chimpanzee)	GPR110	XP_001145329.1
Homo sapiens (human)	GPR110	NP_722582.2
Bos taurus (cattle)	GPR110	NP_001179256.1
Canis lupus familiaris (dog)	GPR110	XP_005627503.1
Mus musculus (house mouse)	Gpr110	NP_598537.2
Rattus norvegicus (Norway rat)	Gpr110	XP_003750709.1

Gene Ontology
Bibliography

Related articles in PubMed

Orphan GPR110 (ADGRF1) targeted by N-docosahexaenoylethanolamine in development of neurons and cognitive function.
Lee JW, Huang BX, Kwon H, Rashid MA, Kharebava G, Desai A, Patnaik S, Marugan J, Kim HY
Nature communications713123(2016 10)

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Araç D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Prömel S, Schöneberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schiöth HB
Pharmacological reviews67(2)338-67(2015)

Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse.
Prömel S, Waller-Evans H, Dixon J, Zahn D, Colledge WH, Doran J, Carlton MB, Grosse J, Schöneberg T, Russ AP, Langenhan T
Developmental dynamics : an official publication of the American Association of Anatomists241(10)1591-602(2012 Oct)

The human and mouse repertoire of the adhesion family of G-protein-coupled receptors.
Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB
Genomics84(1)23-33(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adgrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10208	NM_133776.2 Latest version!	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu54167	XM_006525120.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu54168	XM_006525121.4 Latest version!	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu54168	XM_006525121.3	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu54167	XM_006525120.3	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adgrf1 ( NM_133776.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598537.2 Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), mRNA.

Price & Availability↑

CloneID	OMu10208
Clone ID Related Accession (Same CDS sequence)	NM_133776.2
Accession Version	NM_133776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Mus musculus(house mouse)
Product	adhesion G-protein coupled receptor F1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019869.1, BB661727.1, BB506964.2 and AW113731.1. On Apr 2, 2009 this sequence version replaced NM_133776.1. ##Evidence-Data-START## Transcript exon combination :: BC019217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGAGAATTG GACTTCTGTG GCTCGTCCCT CTCTTCACGC TCACTGAGGG CACCGATGGC 
TTCCTGCAGC AGAAAAATGA TGGCCGAAGA ACAAAAGAAA TTGTCAGTAT GGTTGAGGAA 
AGGCATCCAG TCCACGAGTA TGAGGTGCTA CTGCAGGTGA CATACAGAGA TCCAGAGGAG 
AAGAGAGAAC TGAAGAGATT TCTGAAGCTC TTGAAATCTC CGTCGCCGAG TTTACGTGGT 
CCCAGCAAGA TCATCAGAGT GAAGGCCACC ACATACTGCA GAAGCCGAAA AGGGTTCCTG 
GAGTGTGCCT GCGAAGACAG CTATACGTGG TTTCCTCCCT CCTGCCTTGA CCCCCAGAAC 
TGCTGCCTTC ACACAACTGG ACCCGTCCCA AGCTGCAATT GCTCTCTCAG AGGCCTCAGG 
CAGAGCATTA ATTTCTGTGA GAGAGCAAAA GTTTGGGGCA CTTTTGAAAT CGATGAAAAA 
TTTCCTGAAG ACCTTTGGAA TTCATCATCT GATGTATATG CCCACTACAC AGTTGGAATT 
GAAAATCAAC TTAAAGAAGC GTACAGAAGA GTCCATGGTT TTGAGTCAGT TCGGGTCACC 
CAATTTCGGA AGGGAAGCAT CGTTGTTGGC TACGAAGTGA CCGGCTCCAC TAGCCCTCCA 
GAACTCCTCT TTGCCATTGA ACAGGAGGCC GAAAAGGCAC AGGAGGCCCT AAGGAGGCAG 
TTTCCAGTCA AATATGGCTC TTTTCGAGTA TTCGGGAAAG CTCCGTGTAA CAGCATCTCC 
TTTGGATTCG GGTCTGAGAA TGATGAATAT ACTGTGCCCT GCAGCAGTGG CTTCACAGGA 
AGCATGACGG TCAGGTGCCA GTCCTCTGGG TGGCAGATCA CCAGGGAGTC CTGTGTGCTT 
TCTCAGCTGG AAGAACTGAA GAAGGAGCTC AGGATGATCG CAGGCAAGAT CACAGAGGCG 
GGTGTGGCAT CTTTGGTGCA AAACCTTTCC ACCATCCTTT TGCAAAGTCC ATCGACCACA 
GTTGGGAACC TGGGCTCTGT GGTGTCACTT CTGAGCAACA TTTCATCCCT GTCGCTGGCA 
AACTCTTTAA CTGTGTCCAA TCTGACACTG ATGAACGTCA TCAACATAGC TGATCATATC 
CTGGATTCAG CCTCCATAAC GAACTGGACA ATTTTATTAC AAGATGCAAA GGATGCCAGC 
TCCCAGCTAC TAAAGACATT GGAAAGCATC AGTAGTCTCA TCCCTTCCAT GGCTCTGCCT 
CTTAATTTTT CTGGAAAATT CATTGACTGG AAAGGGACCC CAGTGACCCA AATCCAGAGC 
ACACGGGGCT ACAACTATCA GATGGAAATG AGACAAAATG CCTCTCTGCC TATCCGAGGC 
CACGTGTTTA TTGAACCAGA TCAATTCCAG AAATCGCATC CAAAGACTAT TATCAGCATG 
GCCTCATTGA CCTTTGGGGA CATTCTACCC ATTACCCAGA GAGGAAATGC ATGGGTCAAT 
GGCCCAGTGA TATCCACACT TATTCAAAAC TATTCCATCA GCGAAATTTT CCTGAACTTT 
TCCAAAATAA AGGGAAACCT GACTCAGCCT CGCTGTGTGT TTTGGGATTT TAGCCAGTTG 
CAATGGAGCA ATGCAGGCTG CCAACTGGTT AATGAAACTC TGGACACGGT ACTGTGCCGA 
TGTAGTCACC TGACCTCTTT CTCCATGCTC ATGTCACCCT TTGTCCCCTC TTCTGTTGTT 
CCTGTGGTAA AATGGATCAC GTACATAGGG CTGAGCATCT CCATTGCAAG TCTAATCTTA 
TGCTTGATCA TCGAGTCTCT GTTTTGGAAG CAGACCAAGA GAAGCCAAAC CTCCTACACA 
CGCAACATTT GCCTGGTGAA CATCGCCGTG TCACTCTTAA TTGCTGATGT TTGGTTTATC 
ATTGCTGCCA CTGTGGACCC CTCTGTGAGT CCTTCTGGAG TCTGTGTGGC TGCGGTGTTC 
TTTACCCACT TTTTCTACCT TGCCGTATTC TTTTGGATGC TCGTTCTGGG CATCCTGCTG 
GCTTATCGAA TTATCCTAGT GTTTCATCAC ATGGCTCTGA CTACCATGAT GGCTATTGGC 
TTTTGCCTAG GCTATGGGTG CCCTCTCCTT ATATCCATCA TCACCCTTGC TGTCACCCAA 
CCTAGCAATA GCTATAAAAG GAACGATGTG TGTTGGCTTA ACTGGTCTGA CAAGAGCAAA 
CCTCTCTTGG CGTTCGTTGT TCCCGCGTTG ACTATTGTGG CTGTGAACTT GGTTGTGGTG 
TTGCTGGTCC TCAGGAAGCT TTGGAGGCCA GCAGTTGGAG AAAGACTGAA TCAGGATGAC 
AAGGCCACTG CCATCCGCAT GGGAAAGAGC CTCCTCGTGC TGACCCCGTT GCTAGGGCTC 
ACCTGGGGCT TTGGCATAGG AACAATGGCG AACAGCCACA ATCTGGCTTG GCATGTTCTT 
TTTGCTTTAC TCAATGCTTT CCAGGGATTT TTCATCTTCT GTTTTGGAAT TCTCCTGGAT 
ACCAAGCTGC GGCAACTTCT GTCCAACAAG TTGACTACAT TAAGTTCATG GAAGCAAACA 
TCAAAACGGA ACGCCTCAGA TACAGTTACT CAGCCGAAAT GCTTAAGGAC ATTCAACATA 
CTACAGCACA GAGGCATGTA TGCACTTTCT CATACTGGAG ATTCGTCCAG TGACATCACA 
TTAACCCAGT TTCTATCAAC CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598537.2
CDS	290..3016
Translation	MRIGLLWLVPLFTLTEGTDGFLQQKNDGRRTKEIVSMVEERHPVHEYEVLLQVTYRDPEEKRELKRFLKLLKSPSPSLRGPSKIIRVKATTYCRSRKGFLECACEDSYTWFPPSCLDPQNCCLHTTGPVPSCNCSLRGLRQSINFCERAKVWGTFEIDEKFPEDLWNSSSDVYAHYTVGIENQLKEAYRRVHGFESVRVTQFRKGSIVVGYEVTGSTSPPELLFAIEQEAEKAQEALRRQFPVKYGSFRVFGKAPCNSISFGFGSENDEYTVPCSSGFTGSMTVRCQSSGWQITRESCVLSQLEELKKELRMIAGKITEAGVASLVQNLSTILLQSPSTTVGNLGSVVSLLSNISSLSLANSLTVSNLTLMNVINIADHILDSASITNWTILLQDAKDASSQLLKTLESISSLIPSMALPLNFSGKFIDWKGTPVTQIQSTRGYNYQMEMRQNASLPIRGHVFIEPDQFQKSHPKTIISMASLTFGDILPITQRGNAWVNGPVISTLIQNYSISEIFLNFSKIKGNLTQPRCVFWDFSQLQWSNAGCQLVNETLDTVLCRCSHLTSFSMLMSPFVPSSVVPVVKWITYIGLSISIASLILCLIIESLFWKQTKRSQTSYTRNICLVNIAVSLLIADVWFIIAATVDPSVSPSGVCVAAVFFTHFFYLAVFFWMLVLGILLAYRIILVFHHMALTTMMAIGFCLGYGCPLLISIITLAVTQPSNSYKRNDVCWLNWSDKSKPLLAFVVPALTIVAVNLVVVLLVLRKLWRPAVGERLNQDDKATAIRMGKSLLVLTPLLGLTWGFGIGTMANSHNLAWHVLFALLNAFQGFFIFCFGILLDTKLRQLLSNKLTTLSSWKQTSKRNASDTVTQPKCLRTFNILQHRGMYALSHTGDSSSDITLTQFLSTE

Target ORF information:

RefSeq Version	NM_133776.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133776.2

ORF Insert Sequence:

ATGAGAATTG GACTTCTGTG GCTCGTCCCT CTCTTCACGC TCACTGAGGG CACCGATGGC 
TTCCTGCAGC AGAAAAATGA TGGCCGAAGA ACAAAAGAAA TTGTCAGTAT GGTTGAGGAA 
AGGCATCCAG TCCACGAGTA TGAGGTGCTA CTGCAGGTGA CATACAGAGA TCCAGAGGAG 
AAGAGAGAAC TGAAGAGATT TCTGAAGCTC TTGAAATCTC CGTCGCCGAG TTTACGTGGT 
CCCAGCAAGA TCATCAGAGT GAAGGCCACC ACATACTGCA GAAGCCGAAA AGGGTTCCTG 
GAGTGTGCCT GCGAAGACAG CTATACGTGG TTTCCTCCCT CCTGCCTTGA CCCCCAGAAC 
TGCTGCCTTC ACACAACTGG ACCCGTCCCA AGCTGCAATT GCTCTCTCAG AGGCCTCAGG 
CAGAGCATTA ATTTCTGTGA GAGAGCAAAA GTTTGGGGCA CTTTTGAAAT CGATGAAAAA 
TTTCCTGAAG ACCTTTGGAA TTCATCATCT GATGTATATG CCCACTACAC AGTTGGAATT 
GAAAATCAAC TTAAAGAAGC GTACAGAAGA GTCCATGGTT TTGAGTCAGT TCGGGTCACC 
CAATTTCGGA AGGGAAGCAT CGTTGTTGGC TACGAAGTGA CCGGCTCCAC TAGCCCTCCA 
GAACTCCTCT TTGCCATTGA ACAGGAGGCC GAAAAGGCAC AGGAGGCCCT AAGGAGGCAG 
TTTCCAGTCA AATATGGCTC TTTTCGAGTA TTCGGGAAAG CTCCGTGTAA CAGCATCTCC 
TTTGGATTCG GGTCTGAGAA TGATGAATAT ACTGTGCCCT GCAGCAGTGG CTTCACAGGA 
AGCATGACGG TCAGGTGCCA GTCCTCTGGG TGGCAGATCA CCAGGGAGTC CTGTGTGCTT 
TCTCAGCTGG AAGAACTGAA GAAGGAGCTC AGGATGATCG CAGGCAAGAT CACAGAGGCG 
GGTGTGGCAT CTTTGGTGCA AAACCTTTCC ACCATCCTTT TGCAAAGTCC ATCGACCACA 
GTTGGGAACC TGGGCTCTGT GGTGTCACTT CTGAGCAACA TTTCATCCCT GTCGCTGGCA 
AACTCTTTAA CTGTGTCCAA TCTGACACTG ATGAACGTCA TCAACATAGC TGATCATATC 
CTGGATTCAG CCTCCATAAC GAACTGGACA ATTTTATTAC AAGATGCAAA GGATGCCAGC 
TCCCAGCTAC TAAAGACATT GGAAAGCATC AGTAGTCTCA TCCCTTCCAT GGCTCTGCCT 
CTTAATTTTT CTGGAAAATT CATTGACTGG AAAGGGACCC CAGTGACCCA AATCCAGAGC 
ACACGGGGCT ACAACTATCA GATGGAAATG AGACAAAATG CCTCTCTGCC TATCCGAGGC 
CACGTGTTTA TTGAACCAGA TCAATTCCAG AAATCGCATC CAAAGACTAT TATCAGCATG 
GCCTCATTGA CCTTTGGGGA CATTCTACCC ATTACCCAGA GAGGAAATGC ATGGGTCAAT 
GGCCCAGTGA TATCCACACT TATTCAAAAC TATTCCATCA GCGAAATTTT CCTGAACTTT 
TCCAAAATAA AGGGAAACCT GACTCAGCCT CGCTGTGTGT TTTGGGATTT TAGCCAGTTG 
CAATGGAGCA ATGCAGGCTG CCAACTGGTT AATGAAACTC TGGACACGGT ACTGTGCCGA 
TGTAGTCACC TGACCTCTTT CTCCATGCTC ATGTCACCCT TTGTCCCCTC TTCTGTTGTT 
CCTGTGGTAA AATGGATCAC GTACATAGGG CTGAGCATCT CCATTGCAAG TCTAATCTTA 
TGCTTGATCA TCGAGTCTCT GTTTTGGAAG CAGACCAAGA GAAGCCAAAC CTCCTACACA 
CGCAACATTT GCCTGGTGAA CATCGCCGTG TCACTCTTAA TTGCTGATGT TTGGTTTATC 
ATTGCTGCCA CTGTGGACCC CTCTGTGAGT CCTTCTGGAG TCTGTGTGGC TGCGGTGTTC 
TTTACCCACT TTTTCTACCT TGCCGTATTC TTTTGGATGC TCGTTCTGGG CATCCTGCTG 
GCTTATCGAA TTATCCTAGT GTTTCATCAC ATGGCTCTGA CTACCATGAT GGCTATTGGC 
TTTTGCCTAG GCTATGGGTG CCCTCTCCTT ATATCCATCA TCACCCTTGC TGTCACCCAA 
CCTAGCAATA GCTATAAAAG GAACGATGTG TGTTGGCTTA ACTGGTCTGA CAAGAGCAAA 
CCTCTCTTGG CGTTCGTTGT TCCCGCGTTG ACTATTGTGG CTGTGAACTT GGTTGTGGTG 
TTGCTGGTCC TCAGGAAGCT TTGGAGGCCA GCAGTTGGAG AAAGACTGAA TCAGGATGAC 
AAGGCCACTG CCATCCGCAT GGGAAAGAGC CTCCTCGTGC TGACCCCGTT GCTAGGGCTC 
ACCTGGGGCT TTGGCATAGG AACAATGGCG AACAGCCACA ATCTGGCTTG GCATGTTCTT 
TTTGCTTTAC TCAATGCTTT CCAGGGATTT TTCATCTTCT GTTTTGGAAT TCTCCTGGAT 
ACCAAGCTGC GGCAACTTCT GTCCAACAAG TTGACTACAT TAAGTTCATG GAAGCAAACA 
TCAAAACGGA ACGCCTCAGA TACAGTTACT CAGCCGAAAT GCTTAAGGAC ATTCAACATA 
CTACAGCACA GAGGCATGTA TGCACTTTCT CATACTGGAG ATTCGTCCAG TGACATCACA 
TTAACCCAGT TTCTATCAAC CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf1 ( XM_006525120.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525183.1 Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54167
Clone ID Related Accession (Same CDS sequence)	XM_006525120.4 , XM_006525120.3
Accession Version	XM_006525120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G-protein coupled receptor F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGCAGA AAAATGATGG CCGAAGAACA AAAGAAATTG TCAGTATGGT TGAGGAAAGG 
CATCCAGTCC ACGAGTATGA GGTGCTACTG CAGGTGACAT ACAGAGATCC AGAGGAGAAG 
AGAGAACTGA AGAGATTTCT GAAGCTCTTG AAATCTCCGT CGCCGAGTTT ACGTGGTCCC 
AGCAAGATCA TCAGAGTGAA GGCCACCACA TACTGCAGAA GCCGAAAAGG GTTCCTGGAG 
TGTGCCTGCG AAGACAGCTA TACGTGGTTT CCTCCCTCCT GCCTTGACCC CCAGAACTGC 
TGCCTTCACA CAACTGGACC CGTCCCAAGC TGCAATTGCT CTCTCAGAGG CCTCAGGCAG 
AGCATTAATT TCTGTGAGAG AGCAAAAGTT TGGGGCACTT TTGAAATCGA TGAAAAATTT 
CCTGAAGACC TTTGGAATTC ATCATCTGAT GTATATGCCC ACTACACAGT TGGAATTGAA 
AATCAACTTA AAGAAGCGTA CAGAAGAGTC CATGGTTTTG AGTCAGTTCG GGTCACCCAA 
TTTCGGAAGG GAAGCATCGT TGTTGGCTAC GAAGTGACCG GCTCCACTAG CCCTCCAGAA 
CTCCTCTTTG CCATTGAACA GGAGGCCGAA AAGGCACAGG AGGCCCTAAG GAGGCAGTTT 
CCAGTCAAAT ATGGCTCTTT TCGAGTATTC GGGAAAGCTC CGTGTAACAG CATCTCCTTT 
GGATTCGGGT CTGAGAATGA TGAATATACT GTGCCCTGCA GCAGTGGCTT CACAGGAAGC 
ATGACGGTCA GGTGCCAGTC CTCTGGGTGG CAGATCACCA GGGAGTCCTG TGTGCTTTCT 
CAGCTGGAAG AACTGAAGAA GGAGCTCAGG ATGATCGCAG GCAAGATCAC AGAGGCGGGT 
GTGGCATCTT TGGTGCAAAA CCTTTCCACC ATCCTTTTGC AAAGTCCATC GACCACAGTT 
GGGAACCTGG GCTCTGTGGT GTCACTTCTG AGCAACATTT CATCCCTGTC GCTGGCAAAC 
TCTTTAACTG TGTCCAATCT GACACTGATG AACGTCATCA ACATAGCTGA TCATATCCTG 
GATTCAGCCT CCATAACGAA CTGGACAATT TTATTACAAG ATGCAAAGGA TGCCAGCTCC 
CAGCTACTAA AGACATTGGA AAGCATCAGT AGTCTCATCC CTTCCATGGC TCTGCCTCTT 
AATTTTTCTG GAAAATTCAT TGACTGGAAA GGGACCCCAG TGACCCAAAT CCAGAGCACA 
CGGGGCTACA ACTATCAGAT GGAAATGAGA CAAAATGCCT CTCTGCCTAT CCGAGGCCAC 
GTGTTTATTG AACCAGATCA ATTCCAGAAA TCGCATCCAA AGACTATTAT CAGCATGGCC 
TCATTGACCT TTGGGGACAT TCTACCCATT ACCCAGAGAG GAAATGCATG GGTCAATGGC 
CCAGTGATAT CCACACTTAT TCAAAACTAT TCCATCAGCG AAATTTTCCT GAACTTTTCC 
AAAATAAAGG GAAACCTGAC TCAGCCTCGC TGTGTGTTTT GGGATTTTAG CCAGTTGCAA 
TGGAGCAATG CAGGCTGCCA ACTGGTTAAT GAAACTCTGG ACACGGTACT GTGCCGATGT 
AGTCACCTGA CCTCTTTCTC CATGCTCATG TCACCCTTTG TCCCCTCTTC TGTTGTTCCT 
GTGGTAAAAT GGATCACGTA CATAGGGCTG AGCATCTCCA TTGCAAGTCT AATCTTATGC 
TTGATCATCG AGTCTCTGTT TTGGAAGCAG ACCAAGAGAA GCCAAACCTC CTACACACGC 
AACATTTGCC TGGTGAACAT CGCCGTGTCA CTCTTAATTG CTGATGTTTG GTTTATCATT 
GCTGCCACTG TGGACCCCTC TGTGAGTCCT TCTGGAGTCT GTGTGGCTGC GGTGTTCTTT 
ACCCACTTTT TCTACCTTGC CGTATTCTTT TGGATGCTCG TTCTGGGCAT CCTGCTGGCT 
TATCGAATTA TCCTAGTGTT TCATCACATG GCTCTGACTA CCATGATGGC TATTGGCTTT 
TGCCTAGGCT ATGGGTGCCC TCTCCTTATA TCCATCATCA CCCTTGCTGT CACCCAACCT 
AGCAATAGCT ATAAAAGGAA CGATGTGTGT TGGCTTAACT GGTCTGACAA GAGCAAACCT 
CTCTTGGCGT TCGTTGTTCC CGCGTTGACT ATTGTGGCTG TGAACTTGGT TGTGGTGTTG 
CTGGTCCTCA GGAAGCTTTG GAGGCCAGCA GTTGGAGAAA GACTGAATCA GGATGACAAG 
GCCACTGCCA TCCGCATGGG AAAGAGCCTC CTCGTGCTGA CCCCGTTGCT AGGGCTCACC 
TGGGGCTTTG GCATAGGAAC AATGGCGAAC AGCCACAATC TGGCTTGGCA TGTTCTTTTT 
GCTTTACTCA ATGCTTTCCA GGGATTTTTC ATCTTCTGTT TTGGAATTCT CCTGGATACC 
AAGCTGCGGC AACTTCTGTC CAACAAGTTG ACTACATTAA GTTCATGGAA GCAAACATCA 
AAACGGAACG CCTCAGATAC AGTTACTCAG CCGAAATGCT TAAGGACATT CAACATACTA 
CAGCACAGAG GCATGTATGC ACTTTCTCAT ACTGGAGATT CGTCCAGTGA CATCACATTA 
ACCCAGTTTC TATCAACCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525183.1
CDS	254..3037
Translation	MKFKFAKKQVQRGEKELLDMRIGLLWLVPLFTLTEGTDGFLQQKNDGRRTKEIVSMVEERHPVHEYEVLLQVTYRDPEEKRELKRFLKLLKSPSPSLRGPSKIIRVKATTYCRSRKGFLECACEDSYTWFPPSCLDPQNCCLHTTGPVPSCNCSLRGLRQSINFCERAKVWGTFEIDEKFPEDLWNSSSDVYAHYTVGIENQLKEAYRRVHGFESVRVTQFRKGSIVVGYEVTGSTSPPELLFAIEQEAEKAQEALRRQFPVKYGSFRVFGKAPCNSISFGFGSENDEYTVPCSSGFTGSMTVRCQSSGWQITRESCVLSQLEELKKELRMIAGKITEAGVASLVQNLSTILLQSPSTTVGNLGSVVSLLSNISSLSLANSLTVSNLTLMNVINIADHILDSASITNWTILLQDAKDASSQLLKTLESISSLIPSMALPLNFSGKFIDWKGTPVTQIQSTRGYNYQMEMRQNASLPIRGHVFIEPDQFQKSHPKTIISMASLTFGDILPITQRGNAWVNGPVISTLIQNYSISEIFLNFSKIKGNLTQPRCVFWDFSQLQWSNAGCQLVNETLDTVLCRCSHLTSFSMLMSPFVPSSVVPVVKWITYIGLSISIASLILCLIIESLFWKQTKRSQTSYTRNICLVNIAVSLLIADVWFIIAATVDPSVSPSGVCVAAVFFTHFFYLAVFFWMLVLGILLAYRIILVFHHMALTTMMAIGFCLGYGCPLLISIITLAVTQPSNSYKRNDVCWLNWSDKSKPLLAFVVPALTIVAVNLVVVLLVLRKLWRPAVGERLNQDDKATAIRMGKSLLVLTPLLGLTWGFGIGTMANSHNLAWHVLFALLNAFQGFFIFCFGILLDTKLRQLLSNKLTTLSSWKQTSKRNASDTVTQPKCLRTFNILQHRGMYALSHTGDSSSDITLTQFLSTE

Target ORF information:

RefSeq Version	XM_006525120.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525120.4

ORF Insert Sequence:

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGCAGA AAAATGATGG CCGAAGAACA AAAGAAATTG TCAGTATGGT TGAGGAAAGG 
CATCCAGTCC ACGAGTATGA GGTGCTACTG CAGGTGACAT ACAGAGATCC AGAGGAGAAG 
AGAGAACTGA AGAGATTTCT GAAGCTCTTG AAATCTCCGT CGCCGAGTTT ACGTGGTCCC 
AGCAAGATCA TCAGAGTGAA GGCCACCACA TACTGCAGAA GCCGAAAAGG GTTCCTGGAG 
TGTGCCTGCG AAGACAGCTA TACGTGGTTT CCTCCCTCCT GCCTTGACCC CCAGAACTGC 
TGCCTTCACA CAACTGGACC CGTCCCAAGC TGCAATTGCT CTCTCAGAGG CCTCAGGCAG 
AGCATTAATT TCTGTGAGAG AGCAAAAGTT TGGGGCACTT TTGAAATCGA TGAAAAATTT 
CCTGAAGACC TTTGGAATTC ATCATCTGAT GTATATGCCC ACTACACAGT TGGAATTGAA 
AATCAACTTA AAGAAGCGTA CAGAAGAGTC CATGGTTTTG AGTCAGTTCG GGTCACCCAA 
TTTCGGAAGG GAAGCATCGT TGTTGGCTAC GAAGTGACCG GCTCCACTAG CCCTCCAGAA 
CTCCTCTTTG CCATTGAACA GGAGGCCGAA AAGGCACAGG AGGCCCTAAG GAGGCAGTTT 
CCAGTCAAAT ATGGCTCTTT TCGAGTATTC GGGAAAGCTC CGTGTAACAG CATCTCCTTT 
GGATTCGGGT CTGAGAATGA TGAATATACT GTGCCCTGCA GCAGTGGCTT CACAGGAAGC 
ATGACGGTCA GGTGCCAGTC CTCTGGGTGG CAGATCACCA GGGAGTCCTG TGTGCTTTCT 
CAGCTGGAAG AACTGAAGAA GGAGCTCAGG ATGATCGCAG GCAAGATCAC AGAGGCGGGT 
GTGGCATCTT TGGTGCAAAA CCTTTCCACC ATCCTTTTGC AAAGTCCATC GACCACAGTT 
GGGAACCTGG GCTCTGTGGT GTCACTTCTG AGCAACATTT CATCCCTGTC GCTGGCAAAC 
TCTTTAACTG TGTCCAATCT GACACTGATG AACGTCATCA ACATAGCTGA TCATATCCTG 
GATTCAGCCT CCATAACGAA CTGGACAATT TTATTACAAG ATGCAAAGGA TGCCAGCTCC 
CAGCTACTAA AGACATTGGA AAGCATCAGT AGTCTCATCC CTTCCATGGC TCTGCCTCTT 
AATTTTTCTG GAAAATTCAT TGACTGGAAA GGGACCCCAG TGACCCAAAT CCAGAGCACA 
CGGGGCTACA ACTATCAGAT GGAAATGAGA CAAAATGCCT CTCTGCCTAT CCGAGGCCAC 
GTGTTTATTG AACCAGATCA ATTCCAGAAA TCGCATCCAA AGACTATTAT CAGCATGGCC 
TCATTGACCT TTGGGGACAT TCTACCCATT ACCCAGAGAG GAAATGCATG GGTCAATGGC 
CCAGTGATAT CCACACTTAT TCAAAACTAT TCCATCAGCG AAATTTTCCT GAACTTTTCC 
AAAATAAAGG GAAACCTGAC TCAGCCTCGC TGTGTGTTTT GGGATTTTAG CCAGTTGCAA 
TGGAGCAATG CAGGCTGCCA ACTGGTTAAT GAAACTCTGG ACACGGTACT GTGCCGATGT 
AGTCACCTGA CCTCTTTCTC CATGCTCATG TCACCCTTTG TCCCCTCTTC TGTTGTTCCT 
GTGGTAAAAT GGATCACGTA CATAGGGCTG AGCATCTCCA TTGCAAGTCT AATCTTATGC 
TTGATCATCG AGTCTCTGTT TTGGAAGCAG ACCAAGAGAA GCCAAACCTC CTACACACGC 
AACATTTGCC TGGTGAACAT CGCCGTGTCA CTCTTAATTG CTGATGTTTG GTTTATCATT 
GCTGCCACTG TGGACCCCTC TGTGAGTCCT TCTGGAGTCT GTGTGGCTGC GGTGTTCTTT 
ACCCACTTTT TCTACCTTGC CGTATTCTTT TGGATGCTCG TTCTGGGCAT CCTGCTGGCT 
TATCGAATTA TCCTAGTGTT TCATCACATG GCTCTGACTA CCATGATGGC TATTGGCTTT 
TGCCTAGGCT ATGGGTGCCC TCTCCTTATA TCCATCATCA CCCTTGCTGT CACCCAACCT 
AGCAATAGCT ATAAAAGGAA CGATGTGTGT TGGCTTAACT GGTCTGACAA GAGCAAACCT 
CTCTTGGCGT TCGTTGTTCC CGCGTTGACT ATTGTGGCTG TGAACTTGGT TGTGGTGTTG 
CTGGTCCTCA GGAAGCTTTG GAGGCCAGCA GTTGGAGAAA GACTGAATCA GGATGACAAG 
GCCACTGCCA TCCGCATGGG AAAGAGCCTC CTCGTGCTGA CCCCGTTGCT AGGGCTCACC 
TGGGGCTTTG GCATAGGAAC AATGGCGAAC AGCCACAATC TGGCTTGGCA TGTTCTTTTT 
GCTTTACTCA ATGCTTTCCA GGGATTTTTC ATCTTCTGTT TTGGAATTCT CCTGGATACC 
AAGCTGCGGC AACTTCTGTC CAACAAGTTG ACTACATTAA GTTCATGGAA GCAAACATCA 
AAACGGAACG CCTCAGATAC AGTTACTCAG CCGAAATGCT TAAGGACATT CAACATACTA 
CAGCACAGAG GCATGTATGC ACTTTCTCAT ACTGGAGATT CGTCCAGTGA CATCACATTA 
ACCCAGTTTC TATCAACCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf1 ( XM_006525120.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525183.1 Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54167
Clone ID Related Accession (Same CDS sequence)	XM_006525120.4 , XM_006525120.3
Accession Version	XM_006525120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G-protein coupled receptor F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGCAGA AAAATGATGG CCGAAGAACA AAAGAAATTG TCAGTATGGT TGAGGAAAGG 
CATCCAGTCC ACGAGTATGA GGTGCTACTG CAGGTGACAT ACAGAGATCC AGAGGAGAAG 
AGAGAACTGA AGAGATTTCT GAAGCTCTTG AAATCTCCGT CGCCGAGTTT ACGTGGTCCC 
AGCAAGATCA TCAGAGTGAA GGCCACCACA TACTGCAGAA GCCGAAAAGG GTTCCTGGAG 
TGTGCCTGCG AAGACAGCTA TACGTGGTTT CCTCCCTCCT GCCTTGACCC CCAGAACTGC 
TGCCTTCACA CAACTGGACC CGTCCCAAGC TGCAATTGCT CTCTCAGAGG CCTCAGGCAG 
AGCATTAATT TCTGTGAGAG AGCAAAAGTT TGGGGCACTT TTGAAATCGA TGAAAAATTT 
CCTGAAGACC TTTGGAATTC ATCATCTGAT GTATATGCCC ACTACACAGT TGGAATTGAA 
AATCAACTTA AAGAAGCGTA CAGAAGAGTC CATGGTTTTG AGTCAGTTCG GGTCACCCAA 
TTTCGGAAGG GAAGCATCGT TGTTGGCTAC GAAGTGACCG GCTCCACTAG CCCTCCAGAA 
CTCCTCTTTG CCATTGAACA GGAGGCCGAA AAGGCACAGG AGGCCCTAAG GAGGCAGTTT 
CCAGTCAAAT ATGGCTCTTT TCGAGTATTC GGGAAAGCTC CGTGTAACAG CATCTCCTTT 
GGATTCGGGT CTGAGAATGA TGAATATACT GTGCCCTGCA GCAGTGGCTT CACAGGAAGC 
ATGACGGTCA GGTGCCAGTC CTCTGGGTGG CAGATCACCA GGGAGTCCTG TGTGCTTTCT 
CAGCTGGAAG AACTGAAGAA GGAGCTCAGG ATGATCGCAG GCAAGATCAC AGAGGCGGGT 
GTGGCATCTT TGGTGCAAAA CCTTTCCACC ATCCTTTTGC AAAGTCCATC GACCACAGTT 
GGGAACCTGG GCTCTGTGGT GTCACTTCTG AGCAACATTT CATCCCTGTC GCTGGCAAAC 
TCTTTAACTG TGTCCAATCT GACACTGATG AACGTCATCA ACATAGCTGA TCATATCCTG 
GATTCAGCCT CCATAACGAA CTGGACAATT TTATTACAAG ATGCAAAGGA TGCCAGCTCC 
CAGCTACTAA AGACATTGGA AAGCATCAGT AGTCTCATCC CTTCCATGGC TCTGCCTCTT 
AATTTTTCTG GAAAATTCAT TGACTGGAAA GGGACCCCAG TGACCCAAAT CCAGAGCACA 
CGGGGCTACA ACTATCAGAT GGAAATGAGA CAAAATGCCT CTCTGCCTAT CCGAGGCCAC 
GTGTTTATTG AACCAGATCA ATTCCAGAAA TCGCATCCAA AGACTATTAT CAGCATGGCC 
TCATTGACCT TTGGGGACAT TCTACCCATT ACCCAGAGAG GAAATGCATG GGTCAATGGC 
CCAGTGATAT CCACACTTAT TCAAAACTAT TCCATCAGCG AAATTTTCCT GAACTTTTCC 
AAAATAAAGG GAAACCTGAC TCAGCCTCGC TGTGTGTTTT GGGATTTTAG CCAGTTGCAA 
TGGAGCAATG CAGGCTGCCA ACTGGTTAAT GAAACTCTGG ACACGGTACT GTGCCGATGT 
AGTCACCTGA CCTCTTTCTC CATGCTCATG TCACCCTTTG TCCCCTCTTC TGTTGTTCCT 
GTGGTAAAAT GGATCACGTA CATAGGGCTG AGCATCTCCA TTGCAAGTCT AATCTTATGC 
TTGATCATCG AGTCTCTGTT TTGGAAGCAG ACCAAGAGAA GCCAAACCTC CTACACACGC 
AACATTTGCC TGGTGAACAT CGCCGTGTCA CTCTTAATTG CTGATGTTTG GTTTATCATT 
GCTGCCACTG TGGACCCCTC TGTGAGTCCT TCTGGAGTCT GTGTGGCTGC GGTGTTCTTT 
ACCCACTTTT TCTACCTTGC CGTATTCTTT TGGATGCTCG TTCTGGGCAT CCTGCTGGCT 
TATCGAATTA TCCTAGTGTT TCATCACATG GCTCTGACTA CCATGATGGC TATTGGCTTT 
TGCCTAGGCT ATGGGTGCCC TCTCCTTATA TCCATCATCA CCCTTGCTGT CACCCAACCT 
AGCAATAGCT ATAAAAGGAA CGATGTGTGT TGGCTTAACT GGTCTGACAA GAGCAAACCT 
CTCTTGGCGT TCGTTGTTCC CGCGTTGACT ATTGTGGCTG TGAACTTGGT TGTGGTGTTG 
CTGGTCCTCA GGAAGCTTTG GAGGCCAGCA GTTGGAGAAA GACTGAATCA GGATGACAAG 
GCCACTGCCA TCCGCATGGG AAAGAGCCTC CTCGTGCTGA CCCCGTTGCT AGGGCTCACC 
TGGGGCTTTG GCATAGGAAC AATGGCGAAC AGCCACAATC TGGCTTGGCA TGTTCTTTTT 
GCTTTACTCA ATGCTTTCCA GGGATTTTTC ATCTTCTGTT TTGGAATTCT CCTGGATACC 
AAGCTGCGGC AACTTCTGTC CAACAAGTTG ACTACATTAA GTTCATGGAA GCAAACATCA 
AAACGGAACG CCTCAGATAC AGTTACTCAG CCGAAATGCT TAAGGACATT CAACATACTA 
CAGCACAGAG GCATGTATGC ACTTTCTCAT ACTGGAGATT CGTCCAGTGA CATCACATTA 
ACCCAGTTTC TATCAACCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525183.1
CDS	264..3047
Translation	MKFKFAKKQVQRGEKELLDMRIGLLWLVPLFTLTEGTDGFLQQKNDGRRTKEIVSMVEERHPVHEYEVLLQVTYRDPEEKRELKRFLKLLKSPSPSLRGPSKIIRVKATTYCRSRKGFLECACEDSYTWFPPSCLDPQNCCLHTTGPVPSCNCSLRGLRQSINFCERAKVWGTFEIDEKFPEDLWNSSSDVYAHYTVGIENQLKEAYRRVHGFESVRVTQFRKGSIVVGYEVTGSTSPPELLFAIEQEAEKAQEALRRQFPVKYGSFRVFGKAPCNSISFGFGSENDEYTVPCSSGFTGSMTVRCQSSGWQITRESCVLSQLEELKKELRMIAGKITEAGVASLVQNLSTILLQSPSTTVGNLGSVVSLLSNISSLSLANSLTVSNLTLMNVINIADHILDSASITNWTILLQDAKDASSQLLKTLESISSLIPSMALPLNFSGKFIDWKGTPVTQIQSTRGYNYQMEMRQNASLPIRGHVFIEPDQFQKSHPKTIISMASLTFGDILPITQRGNAWVNGPVISTLIQNYSISEIFLNFSKIKGNLTQPRCVFWDFSQLQWSNAGCQLVNETLDTVLCRCSHLTSFSMLMSPFVPSSVVPVVKWITYIGLSISIASLILCLIIESLFWKQTKRSQTSYTRNICLVNIAVSLLIADVWFIIAATVDPSVSPSGVCVAAVFFTHFFYLAVFFWMLVLGILLAYRIILVFHHMALTTMMAIGFCLGYGCPLLISIITLAVTQPSNSYKRNDVCWLNWSDKSKPLLAFVVPALTIVAVNLVVVLLVLRKLWRPAVGERLNQDDKATAIRMGKSLLVLTPLLGLTWGFGIGTMANSHNLAWHVLFALLNAFQGFFIFCFGILLDTKLRQLLSNKLTTLSSWKQTSKRNASDTVTQPKCLRTFNILQHRGMYALSHTGDSSSDITLTQFLSTE

Target ORF information:

RefSeq Version	XM_006525120.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525120.3

ORF Insert Sequence:

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGCAGA AAAATGATGG CCGAAGAACA AAAGAAATTG TCAGTATGGT TGAGGAAAGG 
CATCCAGTCC ACGAGTATGA GGTGCTACTG CAGGTGACAT ACAGAGATCC AGAGGAGAAG 
AGAGAACTGA AGAGATTTCT GAAGCTCTTG AAATCTCCGT CGCCGAGTTT ACGTGGTCCC 
AGCAAGATCA TCAGAGTGAA GGCCACCACA TACTGCAGAA GCCGAAAAGG GTTCCTGGAG 
TGTGCCTGCG AAGACAGCTA TACGTGGTTT CCTCCCTCCT GCCTTGACCC CCAGAACTGC 
TGCCTTCACA CAACTGGACC CGTCCCAAGC TGCAATTGCT CTCTCAGAGG CCTCAGGCAG 
AGCATTAATT TCTGTGAGAG AGCAAAAGTT TGGGGCACTT TTGAAATCGA TGAAAAATTT 
CCTGAAGACC TTTGGAATTC ATCATCTGAT GTATATGCCC ACTACACAGT TGGAATTGAA 
AATCAACTTA AAGAAGCGTA CAGAAGAGTC CATGGTTTTG AGTCAGTTCG GGTCACCCAA 
TTTCGGAAGG GAAGCATCGT TGTTGGCTAC GAAGTGACCG GCTCCACTAG CCCTCCAGAA 
CTCCTCTTTG CCATTGAACA GGAGGCCGAA AAGGCACAGG AGGCCCTAAG GAGGCAGTTT 
CCAGTCAAAT ATGGCTCTTT TCGAGTATTC GGGAAAGCTC CGTGTAACAG CATCTCCTTT 
GGATTCGGGT CTGAGAATGA TGAATATACT GTGCCCTGCA GCAGTGGCTT CACAGGAAGC 
ATGACGGTCA GGTGCCAGTC CTCTGGGTGG CAGATCACCA GGGAGTCCTG TGTGCTTTCT 
CAGCTGGAAG AACTGAAGAA GGAGCTCAGG ATGATCGCAG GCAAGATCAC AGAGGCGGGT 
GTGGCATCTT TGGTGCAAAA CCTTTCCACC ATCCTTTTGC AAAGTCCATC GACCACAGTT 
GGGAACCTGG GCTCTGTGGT GTCACTTCTG AGCAACATTT CATCCCTGTC GCTGGCAAAC 
TCTTTAACTG TGTCCAATCT GACACTGATG AACGTCATCA ACATAGCTGA TCATATCCTG 
GATTCAGCCT CCATAACGAA CTGGACAATT TTATTACAAG ATGCAAAGGA TGCCAGCTCC 
CAGCTACTAA AGACATTGGA AAGCATCAGT AGTCTCATCC CTTCCATGGC TCTGCCTCTT 
AATTTTTCTG GAAAATTCAT TGACTGGAAA GGGACCCCAG TGACCCAAAT CCAGAGCACA 
CGGGGCTACA ACTATCAGAT GGAAATGAGA CAAAATGCCT CTCTGCCTAT CCGAGGCCAC 
GTGTTTATTG AACCAGATCA ATTCCAGAAA TCGCATCCAA AGACTATTAT CAGCATGGCC 
TCATTGACCT TTGGGGACAT TCTACCCATT ACCCAGAGAG GAAATGCATG GGTCAATGGC 
CCAGTGATAT CCACACTTAT TCAAAACTAT TCCATCAGCG AAATTTTCCT GAACTTTTCC 
AAAATAAAGG GAAACCTGAC TCAGCCTCGC TGTGTGTTTT GGGATTTTAG CCAGTTGCAA 
TGGAGCAATG CAGGCTGCCA ACTGGTTAAT GAAACTCTGG ACACGGTACT GTGCCGATGT 
AGTCACCTGA CCTCTTTCTC CATGCTCATG TCACCCTTTG TCCCCTCTTC TGTTGTTCCT 
GTGGTAAAAT GGATCACGTA CATAGGGCTG AGCATCTCCA TTGCAAGTCT AATCTTATGC 
TTGATCATCG AGTCTCTGTT TTGGAAGCAG ACCAAGAGAA GCCAAACCTC CTACACACGC 
AACATTTGCC TGGTGAACAT CGCCGTGTCA CTCTTAATTG CTGATGTTTG GTTTATCATT 
GCTGCCACTG TGGACCCCTC TGTGAGTCCT TCTGGAGTCT GTGTGGCTGC GGTGTTCTTT 
ACCCACTTTT TCTACCTTGC CGTATTCTTT TGGATGCTCG TTCTGGGCAT CCTGCTGGCT 
TATCGAATTA TCCTAGTGTT TCATCACATG GCTCTGACTA CCATGATGGC TATTGGCTTT 
TGCCTAGGCT ATGGGTGCCC TCTCCTTATA TCCATCATCA CCCTTGCTGT CACCCAACCT 
AGCAATAGCT ATAAAAGGAA CGATGTGTGT TGGCTTAACT GGTCTGACAA GAGCAAACCT 
CTCTTGGCGT TCGTTGTTCC CGCGTTGACT ATTGTGGCTG TGAACTTGGT TGTGGTGTTG 
CTGGTCCTCA GGAAGCTTTG GAGGCCAGCA GTTGGAGAAA GACTGAATCA GGATGACAAG 
GCCACTGCCA TCCGCATGGG AAAGAGCCTC CTCGTGCTGA CCCCGTTGCT AGGGCTCACC 
TGGGGCTTTG GCATAGGAAC AATGGCGAAC AGCCACAATC TGGCTTGGCA TGTTCTTTTT 
GCTTTACTCA ATGCTTTCCA GGGATTTTTC ATCTTCTGTT TTGGAATTCT CCTGGATACC 
AAGCTGCGGC AACTTCTGTC CAACAAGTTG ACTACATTAA GTTCATGGAA GCAAACATCA 
AAACGGAACG CCTCAGATAC AGTTACTCAG CCGAAATGCT TAAGGACATT CAACATACTA 
CAGCACAGAG GCATGTATGC ACTTTCTCAT ACTGGAGATT CGTCCAGTGA CATCACATTA 
ACCCAGTTTC TATCAACCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf1 ( XM_006525121.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525184.1 Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu54168
Clone ID Related Accession (Same CDS sequence)	XM_006525121.4 , XM_006525121.3
Accession Version	XM_006525121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adhesion G-protein coupled receptor F1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGAAAA ATGATGGCCG AAGAACAAAA GAAATTGTCA GTATGGTTGA GGAAAGGCAT 
CCAGTCCACG AGTATGAGGT GCTACTGCAG GTGACATACA GAGATCCAGA GGAGAAGAGA 
GAACTGAAGA GATTTCTGAA GCTCTTGAAA TCTCCGTCGC CGAGTTTACG TGGTCCCAGC 
AAGATCATCA GAGTGAAGGC CACCACATAC TGCAGAAGCC GAAAAGGGTT CCTGGAGTGT 
GCCTGCGAAG ACAGCTATAC GTGGTTTCCT CCCTCCTGCC TTGACCCCCA GAACTGCTGC 
CTTCACACAA CTGGACCCGT CCCAAGCTGC AATTGCTCTC TCAGAGGCCT CAGGCAGAGC 
ATTAATTTCT GTGAGAGAGC AAAAGTTTGG GGCACTTTTG AAATCGATGA AAAATTTCCT 
GAAGACCTTT GGAATTCATC ATCTGATGTA TATGCCCACT ACACAGTTGG AATTGAAAAT 
CAACTTAAAG AAGCGTACAG AAGAGTCCAT GGTTTTGAGT CAGTTCGGGT CACCCAATTT 
CGGAAGGGAA GCATCGTTGT TGGCTACGAA GTGACCGGCT CCACTAGCCC TCCAGAACTC 
CTCTTTGCCA TTGAACAGGA GGCCGAAAAG GCACAGGAGG CCCTAAGGAG GCAGTTTCCA 
GTCAAATATG GCTCTTTTCG AGTATTCGGG AAAGCTCCGT GTAACAGCAT CTCCTTTGGA 
TTCGGGTCTG AGAATGATGA ATATACTGTG CCCTGCAGCA GTGGCTTCAC AGGAAGCATG 
ACGGTCAGGT GCCAGTCCTC TGGGTGGCAG ATCACCAGGG AGTCCTGTGT GCTTTCTCAG 
CTGGAAGAAC TGAAGAAGGA GCTCAGGATG ATCGCAGGCA AGATCACAGA GGCGGGTGTG 
GCATCTTTGG TGCAAAACCT TTCCACCATC CTTTTGCAAA GTCCATCGAC CACAGTTGGG 
AACCTGGGCT CTGTGGTGTC ACTTCTGAGC AACATTTCAT CCCTGTCGCT GGCAAACTCT 
TTAACTGTGT CCAATCTGAC ACTGATGAAC GTCATCAACA TAGCTGATCA TATCCTGGAT 
TCAGCCTCCA TAACGAACTG GACAATTTTA TTACAAGATG CAAAGGATGC CAGCTCCCAG 
CTACTAAAGA CATTGGAAAG CATCAGTAGT CTCATCCCTT CCATGGCTCT GCCTCTTAAT 
TTTTCTGGAA AATTCATTGA CTGGAAAGGG ACCCCAGTGA CCCAAATCCA GAGCACACGG 
GGCTACAACT ATCAGATGGA AATGAGACAA AATGCCTCTC TGCCTATCCG AGGCCACGTG 
TTTATTGAAC CAGATCAATT CCAGAAATCG CATCCAAAGA CTATTATCAG CATGGCCTCA 
TTGACCTTTG GGGACATTCT ACCCATTACC CAGAGAGGAA ATGCATGGGT CAATGGCCCA 
GTGATATCCA CACTTATTCA AAACTATTCC ATCAGCGAAA TTTTCCTGAA CTTTTCCAAA 
ATAAAGGGAA ACCTGACTCA GCCTCGCTGT GTGTTTTGGG ATTTTAGCCA GTTGCAATGG 
AGCAATGCAG GCTGCCAACT GGTTAATGAA ACTCTGGACA CGGTACTGTG CCGATGTAGT 
CACCTGACCT CTTTCTCCAT GCTCATGTCA CCCTTTGTCC CCTCTTCTGT TGTTCCTGTG 
GTAAAATGGA TCACGTACAT AGGGCTGAGC ATCTCCATTG CAAGTCTAAT CTTATGCTTG 
ATCATCGAGT CTCTGTTTTG GAAGCAGACC AAGAGAAGCC AAACCTCCTA CACACGCAAC 
ATTTGCCTGG TGAACATCGC CGTGTCACTC TTAATTGCTG ATGTTTGGTT TATCATTGCT 
GCCACTGTGG ACCCCTCTGT GAGTCCTTCT GGAGTCTGTG TGGCTGCGGT GTTCTTTACC 
CACTTTTTCT ACCTTGCCGT ATTCTTTTGG ATGCTCGTTC TGGGCATCCT GCTGGCTTAT 
CGAATTATCC TAGTGTTTCA TCACATGGCT CTGACTACCA TGATGGCTAT TGGCTTTTGC 
CTAGGCTATG GGTGCCCTCT CCTTATATCC ATCATCACCC TTGCTGTCAC CCAACCTAGC 
AATAGCTATA AAAGGAACGA TGTGTGTTGG CTTAACTGGT CTGACAAGAG CAAACCTCTC 
TTGGCGTTCG TTGTTCCCGC GTTGACTATT GTGGCTGTGA ACTTGGTTGT GGTGTTGCTG 
GTCCTCAGGA AGCTTTGGAG GCCAGCAGTT GGAGAAAGAC TGAATCAGGA TGACAAGGCC 
ACTGCCATCC GCATGGGAAA GAGCCTCCTC GTGCTGACCC CGTTGCTAGG GCTCACCTGG 
GGCTTTGGCA TAGGAACAAT GGCGAACAGC CACAATCTGG CTTGGCATGT TCTTTTTGCT 
TTACTCAATG CTTTCCAGGG ATTTTTCATC TTCTGTTTTG GAATTCTCCT GGATACCAAG 
CTGCGGCAAC TTCTGTCCAA CAAGTTGACT ACATTAAGTT CATGGAAGCA AACATCAAAA 
CGGAACGCCT CAGATACAGT TACTCAGCCG AAATGCTTAA GGACATTCAA CATACTACAG 
CACAGAGGCA TGTATGCACT TTCTCATACT GGAGATTCGT CCAGTGACAT CACATTAACC 
CAGTTTCTAT CAACCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525184.1
CDS	254..3034
Translation	MKFKFAKKQVQRGEKELLDMRIGLLWLVPLFTLTEGTDGFLQKNDGRRTKEIVSMVEERHPVHEYEVLLQVTYRDPEEKRELKRFLKLLKSPSPSLRGPSKIIRVKATTYCRSRKGFLECACEDSYTWFPPSCLDPQNCCLHTTGPVPSCNCSLRGLRQSINFCERAKVWGTFEIDEKFPEDLWNSSSDVYAHYTVGIENQLKEAYRRVHGFESVRVTQFRKGSIVVGYEVTGSTSPPELLFAIEQEAEKAQEALRRQFPVKYGSFRVFGKAPCNSISFGFGSENDEYTVPCSSGFTGSMTVRCQSSGWQITRESCVLSQLEELKKELRMIAGKITEAGVASLVQNLSTILLQSPSTTVGNLGSVVSLLSNISSLSLANSLTVSNLTLMNVINIADHILDSASITNWTILLQDAKDASSQLLKTLESISSLIPSMALPLNFSGKFIDWKGTPVTQIQSTRGYNYQMEMRQNASLPIRGHVFIEPDQFQKSHPKTIISMASLTFGDILPITQRGNAWVNGPVISTLIQNYSISEIFLNFSKIKGNLTQPRCVFWDFSQLQWSNAGCQLVNETLDTVLCRCSHLTSFSMLMSPFVPSSVVPVVKWITYIGLSISIASLILCLIIESLFWKQTKRSQTSYTRNICLVNIAVSLLIADVWFIIAATVDPSVSPSGVCVAAVFFTHFFYLAVFFWMLVLGILLAYRIILVFHHMALTTMMAIGFCLGYGCPLLISIITLAVTQPSNSYKRNDVCWLNWSDKSKPLLAFVVPALTIVAVNLVVVLLVLRKLWRPAVGERLNQDDKATAIRMGKSLLVLTPLLGLTWGFGIGTMANSHNLAWHVLFALLNAFQGFFIFCFGILLDTKLRQLLSNKLTTLSSWKQTSKRNASDTVTQPKCLRTFNILQHRGMYALSHTGDSSSDITLTQFLSTE

Target ORF information:

RefSeq Version	XM_006525121.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525121.4

ORF Insert Sequence:

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGAAAA ATGATGGCCG AAGAACAAAA GAAATTGTCA GTATGGTTGA GGAAAGGCAT 
CCAGTCCACG AGTATGAGGT GCTACTGCAG GTGACATACA GAGATCCAGA GGAGAAGAGA 
GAACTGAAGA GATTTCTGAA GCTCTTGAAA TCTCCGTCGC CGAGTTTACG TGGTCCCAGC 
AAGATCATCA GAGTGAAGGC CACCACATAC TGCAGAAGCC GAAAAGGGTT CCTGGAGTGT 
GCCTGCGAAG ACAGCTATAC GTGGTTTCCT CCCTCCTGCC TTGACCCCCA GAACTGCTGC 
CTTCACACAA CTGGACCCGT CCCAAGCTGC AATTGCTCTC TCAGAGGCCT CAGGCAGAGC 
ATTAATTTCT GTGAGAGAGC AAAAGTTTGG GGCACTTTTG AAATCGATGA AAAATTTCCT 
GAAGACCTTT GGAATTCATC ATCTGATGTA TATGCCCACT ACACAGTTGG AATTGAAAAT 
CAACTTAAAG AAGCGTACAG AAGAGTCCAT GGTTTTGAGT CAGTTCGGGT CACCCAATTT 
CGGAAGGGAA GCATCGTTGT TGGCTACGAA GTGACCGGCT CCACTAGCCC TCCAGAACTC 
CTCTTTGCCA TTGAACAGGA GGCCGAAAAG GCACAGGAGG CCCTAAGGAG GCAGTTTCCA 
GTCAAATATG GCTCTTTTCG AGTATTCGGG AAAGCTCCGT GTAACAGCAT CTCCTTTGGA 
TTCGGGTCTG AGAATGATGA ATATACTGTG CCCTGCAGCA GTGGCTTCAC AGGAAGCATG 
ACGGTCAGGT GCCAGTCCTC TGGGTGGCAG ATCACCAGGG AGTCCTGTGT GCTTTCTCAG 
CTGGAAGAAC TGAAGAAGGA GCTCAGGATG ATCGCAGGCA AGATCACAGA GGCGGGTGTG 
GCATCTTTGG TGCAAAACCT TTCCACCATC CTTTTGCAAA GTCCATCGAC CACAGTTGGG 
AACCTGGGCT CTGTGGTGTC ACTTCTGAGC AACATTTCAT CCCTGTCGCT GGCAAACTCT 
TTAACTGTGT CCAATCTGAC ACTGATGAAC GTCATCAACA TAGCTGATCA TATCCTGGAT 
TCAGCCTCCA TAACGAACTG GACAATTTTA TTACAAGATG CAAAGGATGC CAGCTCCCAG 
CTACTAAAGA CATTGGAAAG CATCAGTAGT CTCATCCCTT CCATGGCTCT GCCTCTTAAT 
TTTTCTGGAA AATTCATTGA CTGGAAAGGG ACCCCAGTGA CCCAAATCCA GAGCACACGG 
GGCTACAACT ATCAGATGGA AATGAGACAA AATGCCTCTC TGCCTATCCG AGGCCACGTG 
TTTATTGAAC CAGATCAATT CCAGAAATCG CATCCAAAGA CTATTATCAG CATGGCCTCA 
TTGACCTTTG GGGACATTCT ACCCATTACC CAGAGAGGAA ATGCATGGGT CAATGGCCCA 
GTGATATCCA CACTTATTCA AAACTATTCC ATCAGCGAAA TTTTCCTGAA CTTTTCCAAA 
ATAAAGGGAA ACCTGACTCA GCCTCGCTGT GTGTTTTGGG ATTTTAGCCA GTTGCAATGG 
AGCAATGCAG GCTGCCAACT GGTTAATGAA ACTCTGGACA CGGTACTGTG CCGATGTAGT 
CACCTGACCT CTTTCTCCAT GCTCATGTCA CCCTTTGTCC CCTCTTCTGT TGTTCCTGTG 
GTAAAATGGA TCACGTACAT AGGGCTGAGC ATCTCCATTG CAAGTCTAAT CTTATGCTTG 
ATCATCGAGT CTCTGTTTTG GAAGCAGACC AAGAGAAGCC AAACCTCCTA CACACGCAAC 
ATTTGCCTGG TGAACATCGC CGTGTCACTC TTAATTGCTG ATGTTTGGTT TATCATTGCT 
GCCACTGTGG ACCCCTCTGT GAGTCCTTCT GGAGTCTGTG TGGCTGCGGT GTTCTTTACC 
CACTTTTTCT ACCTTGCCGT ATTCTTTTGG ATGCTCGTTC TGGGCATCCT GCTGGCTTAT 
CGAATTATCC TAGTGTTTCA TCACATGGCT CTGACTACCA TGATGGCTAT TGGCTTTTGC 
CTAGGCTATG GGTGCCCTCT CCTTATATCC ATCATCACCC TTGCTGTCAC CCAACCTAGC 
AATAGCTATA AAAGGAACGA TGTGTGTTGG CTTAACTGGT CTGACAAGAG CAAACCTCTC 
TTGGCGTTCG TTGTTCCCGC GTTGACTATT GTGGCTGTGA ACTTGGTTGT GGTGTTGCTG 
GTCCTCAGGA AGCTTTGGAG GCCAGCAGTT GGAGAAAGAC TGAATCAGGA TGACAAGGCC 
ACTGCCATCC GCATGGGAAA GAGCCTCCTC GTGCTGACCC CGTTGCTAGG GCTCACCTGG 
GGCTTTGGCA TAGGAACAAT GGCGAACAGC CACAATCTGG CTTGGCATGT TCTTTTTGCT 
TTACTCAATG CTTTCCAGGG ATTTTTCATC TTCTGTTTTG GAATTCTCCT GGATACCAAG 
CTGCGGCAAC TTCTGTCCAA CAAGTTGACT ACATTAAGTT CATGGAAGCA AACATCAAAA 
CGGAACGCCT CAGATACAGT TACTCAGCCG AAATGCTTAA GGACATTCAA CATACTACAG 
CACAGAGGCA TGTATGCACT TTCTCATACT GGAGATTCGT CCAGTGACAT CACATTAACC 
CAGTTTCTAT CAACCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf1 ( XM_006525121.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525184.1 Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.

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CloneID	OMu54168
Clone ID Related Accession (Same CDS sequence)	XM_006525121.4 , XM_006525121.3
Accession Version	XM_006525121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adhesion G-protein coupled receptor F1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGAAAA ATGATGGCCG AAGAACAAAA GAAATTGTCA GTATGGTTGA GGAAAGGCAT 
CCAGTCCACG AGTATGAGGT GCTACTGCAG GTGACATACA GAGATCCAGA GGAGAAGAGA 
GAACTGAAGA GATTTCTGAA GCTCTTGAAA TCTCCGTCGC CGAGTTTACG TGGTCCCAGC 
AAGATCATCA GAGTGAAGGC CACCACATAC TGCAGAAGCC GAAAAGGGTT CCTGGAGTGT 
GCCTGCGAAG ACAGCTATAC GTGGTTTCCT CCCTCCTGCC TTGACCCCCA GAACTGCTGC 
CTTCACACAA CTGGACCCGT CCCAAGCTGC AATTGCTCTC TCAGAGGCCT CAGGCAGAGC 
ATTAATTTCT GTGAGAGAGC AAAAGTTTGG GGCACTTTTG AAATCGATGA AAAATTTCCT 
GAAGACCTTT GGAATTCATC ATCTGATGTA TATGCCCACT ACACAGTTGG AATTGAAAAT 
CAACTTAAAG AAGCGTACAG AAGAGTCCAT GGTTTTGAGT CAGTTCGGGT CACCCAATTT 
CGGAAGGGAA GCATCGTTGT TGGCTACGAA GTGACCGGCT CCACTAGCCC TCCAGAACTC 
CTCTTTGCCA TTGAACAGGA GGCCGAAAAG GCACAGGAGG CCCTAAGGAG GCAGTTTCCA 
GTCAAATATG GCTCTTTTCG AGTATTCGGG AAAGCTCCGT GTAACAGCAT CTCCTTTGGA 
TTCGGGTCTG AGAATGATGA ATATACTGTG CCCTGCAGCA GTGGCTTCAC AGGAAGCATG 
ACGGTCAGGT GCCAGTCCTC TGGGTGGCAG ATCACCAGGG AGTCCTGTGT GCTTTCTCAG 
CTGGAAGAAC TGAAGAAGGA GCTCAGGATG ATCGCAGGCA AGATCACAGA GGCGGGTGTG 
GCATCTTTGG TGCAAAACCT TTCCACCATC CTTTTGCAAA GTCCATCGAC CACAGTTGGG 
AACCTGGGCT CTGTGGTGTC ACTTCTGAGC AACATTTCAT CCCTGTCGCT GGCAAACTCT 
TTAACTGTGT CCAATCTGAC ACTGATGAAC GTCATCAACA TAGCTGATCA TATCCTGGAT 
TCAGCCTCCA TAACGAACTG GACAATTTTA TTACAAGATG CAAAGGATGC CAGCTCCCAG 
CTACTAAAGA CATTGGAAAG CATCAGTAGT CTCATCCCTT CCATGGCTCT GCCTCTTAAT 
TTTTCTGGAA AATTCATTGA CTGGAAAGGG ACCCCAGTGA CCCAAATCCA GAGCACACGG 
GGCTACAACT ATCAGATGGA AATGAGACAA AATGCCTCTC TGCCTATCCG AGGCCACGTG 
TTTATTGAAC CAGATCAATT CCAGAAATCG CATCCAAAGA CTATTATCAG CATGGCCTCA 
TTGACCTTTG GGGACATTCT ACCCATTACC CAGAGAGGAA ATGCATGGGT CAATGGCCCA 
GTGATATCCA CACTTATTCA AAACTATTCC ATCAGCGAAA TTTTCCTGAA CTTTTCCAAA 
ATAAAGGGAA ACCTGACTCA GCCTCGCTGT GTGTTTTGGG ATTTTAGCCA GTTGCAATGG 
AGCAATGCAG GCTGCCAACT GGTTAATGAA ACTCTGGACA CGGTACTGTG CCGATGTAGT 
CACCTGACCT CTTTCTCCAT GCTCATGTCA CCCTTTGTCC CCTCTTCTGT TGTTCCTGTG 
GTAAAATGGA TCACGTACAT AGGGCTGAGC ATCTCCATTG CAAGTCTAAT CTTATGCTTG 
ATCATCGAGT CTCTGTTTTG GAAGCAGACC AAGAGAAGCC AAACCTCCTA CACACGCAAC 
ATTTGCCTGG TGAACATCGC CGTGTCACTC TTAATTGCTG ATGTTTGGTT TATCATTGCT 
GCCACTGTGG ACCCCTCTGT GAGTCCTTCT GGAGTCTGTG TGGCTGCGGT GTTCTTTACC 
CACTTTTTCT ACCTTGCCGT ATTCTTTTGG ATGCTCGTTC TGGGCATCCT GCTGGCTTAT 
CGAATTATCC TAGTGTTTCA TCACATGGCT CTGACTACCA TGATGGCTAT TGGCTTTTGC 
CTAGGCTATG GGTGCCCTCT CCTTATATCC ATCATCACCC TTGCTGTCAC CCAACCTAGC 
AATAGCTATA AAAGGAACGA TGTGTGTTGG CTTAACTGGT CTGACAAGAG CAAACCTCTC 
TTGGCGTTCG TTGTTCCCGC GTTGACTATT GTGGCTGTGA ACTTGGTTGT GGTGTTGCTG 
GTCCTCAGGA AGCTTTGGAG GCCAGCAGTT GGAGAAAGAC TGAATCAGGA TGACAAGGCC 
ACTGCCATCC GCATGGGAAA GAGCCTCCTC GTGCTGACCC CGTTGCTAGG GCTCACCTGG 
GGCTTTGGCA TAGGAACAAT GGCGAACAGC CACAATCTGG CTTGGCATGT TCTTTTTGCT 
TTACTCAATG CTTTCCAGGG ATTTTTCATC TTCTGTTTTG GAATTCTCCT GGATACCAAG 
CTGCGGCAAC TTCTGTCCAA CAAGTTGACT ACATTAAGTT CATGGAAGCA AACATCAAAA 
CGGAACGCCT CAGATACAGT TACTCAGCCG AAATGCTTAA GGACATTCAA CATACTACAG 
CACAGAGGCA TGTATGCACT TTCTCATACT GGAGATTCGT CCAGTGACAT CACATTAACC 
CAGTTTCTAT CAACCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525184.1
CDS	264..3044
Translation	MKFKFAKKQVQRGEKELLDMRIGLLWLVPLFTLTEGTDGFLQKNDGRRTKEIVSMVEERHPVHEYEVLLQVTYRDPEEKRELKRFLKLLKSPSPSLRGPSKIIRVKATTYCRSRKGFLECACEDSYTWFPPSCLDPQNCCLHTTGPVPSCNCSLRGLRQSINFCERAKVWGTFEIDEKFPEDLWNSSSDVYAHYTVGIENQLKEAYRRVHGFESVRVTQFRKGSIVVGYEVTGSTSPPELLFAIEQEAEKAQEALRRQFPVKYGSFRVFGKAPCNSISFGFGSENDEYTVPCSSGFTGSMTVRCQSSGWQITRESCVLSQLEELKKELRMIAGKITEAGVASLVQNLSTILLQSPSTTVGNLGSVVSLLSNISSLSLANSLTVSNLTLMNVINIADHILDSASITNWTILLQDAKDASSQLLKTLESISSLIPSMALPLNFSGKFIDWKGTPVTQIQSTRGYNYQMEMRQNASLPIRGHVFIEPDQFQKSHPKTIISMASLTFGDILPITQRGNAWVNGPVISTLIQNYSISEIFLNFSKIKGNLTQPRCVFWDFSQLQWSNAGCQLVNETLDTVLCRCSHLTSFSMLMSPFVPSSVVPVVKWITYIGLSISIASLILCLIIESLFWKQTKRSQTSYTRNICLVNIAVSLLIADVWFIIAATVDPSVSPSGVCVAAVFFTHFFYLAVFFWMLVLGILLAYRIILVFHHMALTTMMAIGFCLGYGCPLLISIITLAVTQPSNSYKRNDVCWLNWSDKSKPLLAFVVPALTIVAVNLVVVLLVLRKLWRPAVGERLNQDDKATAIRMGKSLLVLTPLLGLTWGFGIGTMANSHNLAWHVLFALLNAFQGFFIFCFGILLDTKLRQLLSNKLTTLSSWKQTSKRNASDTVTQPKCLRTFNILQHRGMYALSHTGDSSSDITLTQFLSTE

Target ORF information:

RefSeq Version	XM_006525121.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor F1 (Adgrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525121.3

ORF Insert Sequence:

ATGAAGTTTA AGTTTGCCAA GAAGCAAGTC CAGAGAGGAG AGAAGGAGCT ACTGGATATG 
AGAATTGGAC TTCTGTGGCT CGTCCCTCTC TTCACGCTCA CTGAGGGCAC CGATGGCTTC 
CTGCAGAAAA ATGATGGCCG AAGAACAAAA GAAATTGTCA GTATGGTTGA GGAAAGGCAT 
CCAGTCCACG AGTATGAGGT GCTACTGCAG GTGACATACA GAGATCCAGA GGAGAAGAGA 
GAACTGAAGA GATTTCTGAA GCTCTTGAAA TCTCCGTCGC CGAGTTTACG TGGTCCCAGC 
AAGATCATCA GAGTGAAGGC CACCACATAC TGCAGAAGCC GAAAAGGGTT CCTGGAGTGT 
GCCTGCGAAG ACAGCTATAC GTGGTTTCCT CCCTCCTGCC TTGACCCCCA GAACTGCTGC 
CTTCACACAA CTGGACCCGT CCCAAGCTGC AATTGCTCTC TCAGAGGCCT CAGGCAGAGC 
ATTAATTTCT GTGAGAGAGC AAAAGTTTGG GGCACTTTTG AAATCGATGA AAAATTTCCT 
GAAGACCTTT GGAATTCATC ATCTGATGTA TATGCCCACT ACACAGTTGG AATTGAAAAT 
CAACTTAAAG AAGCGTACAG AAGAGTCCAT GGTTTTGAGT CAGTTCGGGT CACCCAATTT 
CGGAAGGGAA GCATCGTTGT TGGCTACGAA GTGACCGGCT CCACTAGCCC TCCAGAACTC 
CTCTTTGCCA TTGAACAGGA GGCCGAAAAG GCACAGGAGG CCCTAAGGAG GCAGTTTCCA 
GTCAAATATG GCTCTTTTCG AGTATTCGGG AAAGCTCCGT GTAACAGCAT CTCCTTTGGA 
TTCGGGTCTG AGAATGATGA ATATACTGTG CCCTGCAGCA GTGGCTTCAC AGGAAGCATG 
ACGGTCAGGT GCCAGTCCTC TGGGTGGCAG ATCACCAGGG AGTCCTGTGT GCTTTCTCAG 
CTGGAAGAAC TGAAGAAGGA GCTCAGGATG ATCGCAGGCA AGATCACAGA GGCGGGTGTG 
GCATCTTTGG TGCAAAACCT TTCCACCATC CTTTTGCAAA GTCCATCGAC CACAGTTGGG 
AACCTGGGCT CTGTGGTGTC ACTTCTGAGC AACATTTCAT CCCTGTCGCT GGCAAACTCT 
TTAACTGTGT CCAATCTGAC ACTGATGAAC GTCATCAACA TAGCTGATCA TATCCTGGAT 
TCAGCCTCCA TAACGAACTG GACAATTTTA TTACAAGATG CAAAGGATGC CAGCTCCCAG 
CTACTAAAGA CATTGGAAAG CATCAGTAGT CTCATCCCTT CCATGGCTCT GCCTCTTAAT 
TTTTCTGGAA AATTCATTGA CTGGAAAGGG ACCCCAGTGA CCCAAATCCA GAGCACACGG 
GGCTACAACT ATCAGATGGA AATGAGACAA AATGCCTCTC TGCCTATCCG AGGCCACGTG 
TTTATTGAAC CAGATCAATT CCAGAAATCG CATCCAAAGA CTATTATCAG CATGGCCTCA 
TTGACCTTTG GGGACATTCT ACCCATTACC CAGAGAGGAA ATGCATGGGT CAATGGCCCA 
GTGATATCCA CACTTATTCA AAACTATTCC ATCAGCGAAA TTTTCCTGAA CTTTTCCAAA 
ATAAAGGGAA ACCTGACTCA GCCTCGCTGT GTGTTTTGGG ATTTTAGCCA GTTGCAATGG 
AGCAATGCAG GCTGCCAACT GGTTAATGAA ACTCTGGACA CGGTACTGTG CCGATGTAGT 
CACCTGACCT CTTTCTCCAT GCTCATGTCA CCCTTTGTCC CCTCTTCTGT TGTTCCTGTG 
GTAAAATGGA TCACGTACAT AGGGCTGAGC ATCTCCATTG CAAGTCTAAT CTTATGCTTG 
ATCATCGAGT CTCTGTTTTG GAAGCAGACC AAGAGAAGCC AAACCTCCTA CACACGCAAC 
ATTTGCCTGG TGAACATCGC CGTGTCACTC TTAATTGCTG ATGTTTGGTT TATCATTGCT 
GCCACTGTGG ACCCCTCTGT GAGTCCTTCT GGAGTCTGTG TGGCTGCGGT GTTCTTTACC 
CACTTTTTCT ACCTTGCCGT ATTCTTTTGG ATGCTCGTTC TGGGCATCCT GCTGGCTTAT 
CGAATTATCC TAGTGTTTCA TCACATGGCT CTGACTACCA TGATGGCTAT TGGCTTTTGC 
CTAGGCTATG GGTGCCCTCT CCTTATATCC ATCATCACCC TTGCTGTCAC CCAACCTAGC 
AATAGCTATA AAAGGAACGA TGTGTGTTGG CTTAACTGGT CTGACAAGAG CAAACCTCTC 
TTGGCGTTCG TTGTTCCCGC GTTGACTATT GTGGCTGTGA ACTTGGTTGT GGTGTTGCTG 
GTCCTCAGGA AGCTTTGGAG GCCAGCAGTT GGAGAAAGAC TGAATCAGGA TGACAAGGCC 
ACTGCCATCC GCATGGGAAA GAGCCTCCTC GTGCTGACCC CGTTGCTAGG GCTCACCTGG 
GGCTTTGGCA TAGGAACAAT GGCGAACAGC CACAATCTGG CTTGGCATGT TCTTTTTGCT 
TTACTCAATG CTTTCCAGGG ATTTTTCATC TTCTGTTTTG GAATTCTCCT GGATACCAAG 
CTGCGGCAAC TTCTGTCCAA CAAGTTGACT ACATTAAGTT CATGGAAGCA AACATCAAAA 
CGGAACGCCT CAGATACAGT TACTCAGCCG AAATGCTTAA GGACATTCAA CATACTACAG 
CACAGAGGCA TGTATGCACT TTCTCATACT GGAGATTCGT CCAGTGACAT CACATTAACC 
CAGTTTCTAT CAACCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Orphan GPR110 (ADGRF1) targeted by N-docosahexaenoylethanolamine in development of neurons and cognitive function.
Nature communications713123(2016 10)
Lee JW,Huang BX,Kwon H,Rashid MA,Kharebava G,Desai A,Patnaik S,Marugan J,Kim HY

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Araç D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Prömel S,Schöneberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schiöth HB

Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse.
Developmental dynamics : an official publication of the American Association of Anatomists241(10)1591-602(2012 Oct)
Prömel S,Waller-Evans H,Dixon J,Zahn D,Colledge WH,Doran J,Carlton MB,Grosse J,Schöneberg T,Russ AP,Langenhan T

The human and mouse repertoire of the adhesion family of G-protein-coupled receptors.
Genomics84(1)23-33(2004 Jul)
Bjarnadóttir TK,Fredriksson R,Höglund PJ,Gloriam DE,Lagerström MC,Schiöth HB

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Adgrf1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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