Rtf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rtf1
Entrez Gene ID	76246
Full Name	RTF1, Paf1/RNA polymerase II complex component
Synonyms	2900005O08Rik,6530416A09Rik,AI853581,AU043144,AW553985,Gtl7,cbp82
General protein information	Preferred Names RTF1, Paf1/RNA polymerase II complex component Names RNA polymerase-associated protein RTF1 homolog Rtf1, Paf1/RNA polymerase II complex component, homolog gene trap locus 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_030112.2	NP_084388.2	RNA polymerase-associated protein RTF1 homolog precursor
XM_017319319.1	XP_017174808.1	RNA polymerase-associated protein RTF1 homolog isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RTF1	NP_001179414.1
Mus musculus (house mouse)	Rtf1	NP_084388.2
Drosophila melanogaster (fruit fly)	Rtf1	NP_611665.1
Macaca mulatta (Rhesus monkey)	RTF1	XP_001100507.1
Gallus gallus (chicken)	RTF1	XP_421139.3
Pan troglodytes (chimpanzee)	RTF1	XP_001142072.1
Canis lupus familiaris (dog)	RTF1	XP_544630.2
Xenopus tropicalis (tropical clawed frog)	rtf1	NP_001005068.1
Danio rerio (zebrafish)	rtf1	NP_001077046.1
Homo sapiens (human)	RTF1	NP_055953.3

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto AL, Yang Y, Ntziachristos P, Rothbart S, Buckley S, Dolgalev I, Stadtfeld M, Strahl BD, Dynlacht BD, Tsirigos A, Aifantis I
Nature cell biology18(11)1127-1138(2016 Nov)

CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.
Zhang K, Haversat JM, Mager J
Developmental biology383(1)15-27(2013 Nov)

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.
Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, de Vries I, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Iacone R, Anastassiadis K, Stewart AF, Pisabarro MT, Caldarelli A, Poser I, Theis M, Buchholz F
Cell stem cell4(5)403-15(2009 May)

Structure and DNA binding of the human Rtf1 Plus3 domain.
de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE
Structure (London, England : 1993)16(1)149-59(2008 Jan)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rtf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rtf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21521	NM_030112.2 Latest version!	Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu31003	XM_017319319.1 Latest version!	Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rtf1 ( NM_030112.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084388.2 Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), mRNA.

Price & Availability↑

CloneID	OMu21521
Clone ID Related Accession (Same CDS sequence)	NM_030112.2
Accession Version	NM_030112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Mus musculus(house mouse)
Product	RNA polymerase-associated protein RTF1 homolog precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844536.13, BY270385.1, AK019305.1, BC117849.1, CN839001.1, CN532066.1 and AW491091.1. On Aug 12, 2008 this sequence version replaced NM_030112.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: BC117849.1, SRR7345562.1163660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGTC GCCTTTGTGT GGGTCGAGCG GCGGCGGTGG CGGCGGCGGT GGCGGCGGCG 
GCAGTGGCGG TCCCACTGGC AGGCGGGCAA GAGGGGAGTC AGGGCGGCGT CCGGCGTGGA 
AGCCGGGGGA CCACCATGGT AAAGAAGCGG AAAGGCCGTG TCGTGATCGA CTCGGATACG 
GAAGACAGCG GCAGTGACGA GAACCTGGAT CAGGAGCTCT TGTCCCTGGC GAAACGGAAG 
CGCAGTGACT CCGAGGAGAA GGAGCCTCCT GTGAGTCAGC CTGCAGCCTC ATCAGATTCA 
GAGACCTCCG ACAGTGATGA TGAGTGGACA TTTGGGAGCA ATAAAAATAA GAAGAAAGGA 
AAAACCAGAA AAGTAGAGAA GAAAGGAGCC ATGAAGAAGC AAGCCAACAA AGCTGCCTCC 
TCGGGCAGCT CAGACAGAGA CAGCTCAGCT GAGAGCTCTG CCCCTGAGGA AGGTGAAGTG 
TCAGATTCTG AGAGCAGCAG CTCCTCTTCC AGTTCAGATT CAGACTCCTC GTCAGAAGAT 
GAAGAATTCC ATGATGGCTA TGGAGAGGAT CTTATGGGAG ATGAGGAAGA TAGGGCCCGA 
CTGGAGCAGA TGACGGAGAA GGAGAGGGAG CAGGAGCTCT TCAACCGCAT AGAGAAGAGA 
GAGGTGTTGA AAAGAAGATT TGAAATTAAG AAAAAACTAA AAACAGCCAA AAAGAAAGAG 
AAGAAAGAAA AAAAGAAAAA GCAGGAAGAA GAACAAGAAA AGAAGAAGCT GACACAGATT 
CAGGAATCTC AGGTTACTTC CCACAATAAA GAACGGCGTT CCAAGCGGGA TGAAAAACTA 
GATAAGAAAT CTCAAGCCAT GGAGGAGCTA AAAGCAGAGC GAGAAAAACG GAAGAACAGA 
ACAGCTGAGC TCCTCGCCAA AAAACAGCCA TTGAAAACCA GTGAGGTCTA CTCTGACGAT 
GAGGAGGAGG AAGATGATGA CAAGTCCAGT GAGAAATCGG ACCGCTCATC CCGGACATCA 
TCATCTGATG AAGAGGAAGA GAAAGAAGAA ATCCCTCCAA AATCACAGCC AGTCTCATTA 
CCCGAGGAAC TGAATCGGGT TCGGTTATCA CGGCATAAGC TAGAGCGCTG GTGTCACATG 
CCCTTCTTTG CTAAAACTGT CACAGGATGT TTTGTACGGA TTGGCATTGG AAACCACAAT 
AGCAAGCCAG TTTACCGGGT GGCTGAGATC ACGGGTGTTG TGGAAACTGC CAAAGTTTAC 
CAGCTGGGTG GCACCAGAAC AAATAAAGGG CTCCAGCTAC GGCATGGCAA TGACCAGCGA 
GTGTTCCGCC TAGAGTTTGT GTCCAACCAA GAATTCACTG AAAGTGAATT CATGAAGTGG 
AAAGAAGCGA TGTTTTCTGC TGGCATGCAG TTGCCCACAC TAGATGAAAT CAATAAAAAG 
GAATTATCTA TTAAGGAAGC TCTCAATTAT AAATTCAATG ATCAGGACAT TGAGGAGATT 
GTAAAAGAGA AGGAAAGGTT CAGAAAAGCC CCACCAAACT ATGCTATGAA GAAGACTCAG 
CTGCTAAAGG AGAAGGCTAT GGCTGAGGAC CTGGGAGATC AGGACAAAGC CAAACAAATC 
CAAGACCAGC TAAATGAGCT AGAGGAGCGA GCAGAGGCCC TGGACCGCCA GCGGACCAAG 
AACATATCTG CTATCAGCTA CATCAACCAG CGGAATCGGG AGTGGAATAT TGTGGAGTCT 
GAGAAGGCTC TGGTGGCTGA AAGTCACAAC ATGAGAAACC AACAGATGGA CCCCTTCACC 
AGGCGGCAGT GCAAACCCAC CATCGTCTCT AATTCCAGGG ATCCAGCTGT TCAGGCTGCC 
ATTTTGGCCC AGCTCAATGC AAAATACGGT TCTGGGGTAT TACCAGATGC TCCAAAGGAA 
ATGAGCAAGG GTCAAGGAAA GGATAAAGAT TTGAATTCTA AGACAGCCAG TGACCTCTCG 
GAAGACCTGT TCAAAGTGCA TGACTTTGAC GTGAAGATTG ACTTACAGGT TCCAAGCTCA 
GAGTCAAAAG CTTTGGCCAT TACCTCCAAA GCCCCACCAG CCAAGGACGG AGCTCCGAGG 
AGATCTCTGA ACTTAGAAGA CTACAAAAAA CGACGGGGGC TTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084388.2
CDS	1..2148
Translation	MRGRLCVGRAAAVAAAVAAAAVAVPLAGGQEGSQGGVRRGSRGTTMVKKRKGRVVIDSDTEDSGSDENLDQELLSLAKRKRSDSEEKEPPVSQPAASSDSETSDSDDEWTFGSNKNKKKGKTRKVEKKGAMKKQANKAASSGSSDRDSSAESSAPEEGEVSDSESSSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEDDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTKNISAISYINQRNREWNIVESEKALVAESHNMRNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYGSGVLPDAPKEMSKGQGKDKDLNSKTASDLSEDLFKVHDFDVKIDLQVPSSESKALAITSKAPPAKDGAPRRSLNLEDYKKRRGLI

Target ORF information:

RefSeq Version	NM_030112.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030112.2

ORF Insert Sequence:

ATGCGCGGTC GCCTTTGTGT GGGTCGAGCG GCGGCGGTGG CGGCGGCGGT GGCGGCGGCG 
GCAGTGGCGG TCCCACTGGC AGGCGGGCAA GAGGGGAGTC AGGGCGGCGT CCGGCGTGGA 
AGCCGGGGGA CCACCATGGT AAAGAAGCGG AAAGGCCGTG TCGTGATCGA CTCGGATACG 
GAAGACAGCG GCAGTGACGA GAACCTGGAT CAGGAGCTCT TGTCCCTGGC GAAACGGAAG 
CGCAGTGACT CCGAGGAGAA GGAGCCTCCT GTGAGTCAGC CTGCAGCCTC ATCAGATTCA 
GAGACCTCCG ACAGTGATGA TGAGTGGACA TTTGGGAGCA ATAAAAATAA GAAGAAAGGA 
AAAACCAGAA AAGTAGAGAA GAAAGGAGCC ATGAAGAAGC AAGCCAACAA AGCTGCCTCC 
TCGGGCAGCT CAGACAGAGA CAGCTCAGCT GAGAGCTCTG CCCCTGAGGA AGGTGAAGTG 
TCAGATTCTG AGAGCAGCAG CTCCTCTTCC AGTTCAGATT CAGACTCCTC GTCAGAAGAT 
GAAGAATTCC ATGATGGCTA TGGAGAGGAT CTTATGGGAG ATGAGGAAGA TAGGGCCCGA 
CTGGAGCAGA TGACGGAGAA GGAGAGGGAG CAGGAGCTCT TCAACCGCAT AGAGAAGAGA 
GAGGTGTTGA AAAGAAGATT TGAAATTAAG AAAAAACTAA AAACAGCCAA AAAGAAAGAG 
AAGAAAGAAA AAAAGAAAAA GCAGGAAGAA GAACAAGAAA AGAAGAAGCT GACACAGATT 
CAGGAATCTC AGGTTACTTC CCACAATAAA GAACGGCGTT CCAAGCGGGA TGAAAAACTA 
GATAAGAAAT CTCAAGCCAT GGAGGAGCTA AAAGCAGAGC GAGAAAAACG GAAGAACAGA 
ACAGCTGAGC TCCTCGCCAA AAAACAGCCA TTGAAAACCA GTGAGGTCTA CTCTGACGAT 
GAGGAGGAGG AAGATGATGA CAAGTCCAGT GAGAAATCGG ACCGCTCATC CCGGACATCA 
TCATCTGATG AAGAGGAAGA GAAAGAAGAA ATCCCTCCAA AATCACAGCC AGTCTCATTA 
CCCGAGGAAC TGAATCGGGT TCGGTTATCA CGGCATAAGC TAGAGCGCTG GTGTCACATG 
CCCTTCTTTG CTAAAACTGT CACAGGATGT TTTGTACGGA TTGGCATTGG AAACCACAAT 
AGCAAGCCAG TTTACCGGGT GGCTGAGATC ACGGGTGTTG TGGAAACTGC CAAAGTTTAC 
CAGCTGGGTG GCACCAGAAC AAATAAAGGG CTCCAGCTAC GGCATGGCAA TGACCAGCGA 
GTGTTCCGCC TAGAGTTTGT GTCCAACCAA GAATTCACTG AAAGTGAATT CATGAAGTGG 
AAAGAAGCGA TGTTTTCTGC TGGCATGCAG TTGCCCACAC TAGATGAAAT CAATAAAAAG 
GAATTATCTA TTAAGGAAGC TCTCAATTAT AAATTCAATG ATCAGGACAT TGAGGAGATT 
GTAAAAGAGA AGGAAAGGTT CAGAAAAGCC CCACCAAACT ATGCTATGAA GAAGACTCAG 
CTGCTAAAGG AGAAGGCTAT GGCTGAGGAC CTGGGAGATC AGGACAAAGC CAAACAAATC 
CAAGACCAGC TAAATGAGCT AGAGGAGCGA GCAGAGGCCC TGGACCGCCA GCGGACCAAG 
AACATATCTG CTATCAGCTA CATCAACCAG CGGAATCGGG AGTGGAATAT TGTGGAGTCT 
GAGAAGGCTC TGGTGGCTGA AAGTCACAAC ATGAGAAACC AACAGATGGA CCCCTTCACC 
AGGCGGCAGT GCAAACCCAC CATCGTCTCT AATTCCAGGG ATCCAGCTGT TCAGGCTGCC 
ATTTTGGCCC AGCTCAATGC AAAATACGGT TCTGGGGTAT TACCAGATGC TCCAAAGGAA 
ATGAGCAAGG GTCAAGGAAA GGATAAAGAT TTGAATTCTA AGACAGCCAG TGACCTCTCG 
GAAGACCTGT TCAAAGTGCA TGACTTTGAC GTGAAGATTG ACTTACAGGT TCCAAGCTCA 
GAGTCAAAAG CTTTGGCCAT TACCTCCAAA GCCCCACCAG CCAAGGACGG AGCTCCGAGG 
AGATCTCTGA ACTTAGAAGA CTACAAAAAA CGACGGGGGC TTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtf1 ( XM_017319319.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174808.1 Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31003
Clone ID Related Accession (Same CDS sequence)	XM_017319319.1
Accession Version	XM_017319319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	RNA polymerase-associated protein RTF1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AGGAAGATAG GGCCCGACTG GAGCAGATGA CGGAGAAGGA GAGGGAGCAG 
GAGCTCTTCA ACCGCATAGA GAAGAGAGAG GTGTTGAAAA GAAGATTTGA AATTAAGAAA 
AAACTAAAAA CAGCCAAAAA GAAAGAGAAG AAAGAAAAAA AGAAAAAGCA GGAAGAAGAA 
CAAGAAAAGA AGAAGCTGAC ACAGATTCAG GAATCTCAGG TTACTTCCCA CAATAAAGAA 
CGGCGTTCCA AGCGGGATGA AAAACTAGAT AAGAAATCTC AAGCCATGGA GGAGCTAAAA 
GCAGAGCGAG AAAAACGGAA GAACAGAACA GCTGAGCTCC TCGCCAAAAA ACAGCCATTG 
AAAACCAGTG AGGTCTACTC TGACGATGAG GAGGAGGAAG ATGATGACAA GTCCAGTGAG 
AAATCGGACC GCTCATCCCG GACATCATCA TCTGATGAAG AGGAAGAGAA AGAAGAAATC 
CCTCCAAAAT CACAGCCAGT CTCATTACCC GAGGAACTGA ATCGGGTTCG GTTATCACGG 
CATAAGCTAG AGCGCTGGTG TCACATGCCC TTCTTTGCTA AAACTGTCAC AGGATGTTTT 
GTACGGATTG GCATTGGAAA CCACAATAGC AAGCCAGTTT ACCGGGTGGC TGAGATCACG 
GGTGTTGTGG AAACTGCCAA AGTTTACCAG CTGGGTGGCA CCAGAACAAA TAAAGGGCTC 
CAGCTACGGC ATGGCAATGA CCAGCGAGTG TTCCGCCTAG AGTTTGTGTC CAACCAAGAA 
TTCACTGAAA GTGAATTCAT GAAGTGGAAA GAAGCGATGT TTTCTGCTGG CATGCAGTTG 
CCCACACTAG ATGAAATCAA TAAAAAGGAA TTATCTATTA AGGAAGCTCT CAATTATAAA 
TTCAATGATC AGGACATTGA GGAGATTGTA AAAGAGAAGG AAAGGTTCAG AAAAGCCCCA 
CCAAACTATG CTATGAAGAA GACTCAGCTG CTAAAGGAGA AGGCTATGGC TGAGGACCTG 
GGAGATCAGG ACAAAGCCAA ACAAATCCAA GACCAGCTAA ATGAGCTAGA GGAGCGAGCA 
GAGGCCCTGG ACCGCCAGCG GACCAAGAAC ATATCTGCTA TCAGCTACAT CAACCAGCGG 
AATCGGGAGT GGAATATTGT GGAGTCTGAG AAGGCTCTGG TGGCTGAAAG TCACAACATG 
AGAAACCAAC AGATGGACCC CTTCACCAGG CGGCAGTGCA AACCCACCAT CGTCTCTAAT 
TCCAGGGATC CAGCTGTTCA GGCTGCCATT TTGGCCCAGC TCAATGCAAA ATACGGTTCT 
GGGGTATTAC CAGATGCTCC AAAGGAAATG AGCAAGGGTC AAGGAAAGGA TAAAGATTTG 
AATTCTAAGA CAGCCAGTGA CCTCTCGGAA GACCTGTTCA AAGTGCATGA CTTTGACGTG 
AAGATTGACT TACAGGTTCC AAGCTCAGAG TCAAAAGCTT TGGCCATTAC CTCCAAAGCC 
CCACCAGCCA AGGACGGAGC TCCGAGGAGA TCTCTGAACT TAGAAGACTA CAAAAAACGA 
CGGGGGCTTA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174808.1
CDS	170..1744
Translation	MGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEDDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAEDLGDQDKAKQIQDQLNELEERAEALDRQRTKNISAISYINQRNREWNIVESEKALVAESHNMRNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYGSGVLPDAPKEMSKGQGKDKDLNSKTASDLSEDLFKVHDFDVKIDLQVPSSESKALAITSKAPPAKDGAPRRSLNLEDYKKRRGLI

Target ORF information:

RefSeq Version	XM_017319319.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RTF1, Paf1/RNA polymerase II complex component (Rtf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319319.1

ORF Insert Sequence:

ATGGGAGATG AGGAAGATAG GGCCCGACTG GAGCAGATGA CGGAGAAGGA GAGGGAGCAG 
GAGCTCTTCA ACCGCATAGA GAAGAGAGAG GTGTTGAAAA GAAGATTTGA AATTAAGAAA 
AAACTAAAAA CAGCCAAAAA GAAAGAGAAG AAAGAAAAAA AGAAAAAGCA GGAAGAAGAA 
CAAGAAAAGA AGAAGCTGAC ACAGATTCAG GAATCTCAGG TTACTTCCCA CAATAAAGAA 
CGGCGTTCCA AGCGGGATGA AAAACTAGAT AAGAAATCTC AAGCCATGGA GGAGCTAAAA 
GCAGAGCGAG AAAAACGGAA GAACAGAACA GCTGAGCTCC TCGCCAAAAA ACAGCCATTG 
AAAACCAGTG AGGTCTACTC TGACGATGAG GAGGAGGAAG ATGATGACAA GTCCAGTGAG 
AAATCGGACC GCTCATCCCG GACATCATCA TCTGATGAAG AGGAAGAGAA AGAAGAAATC 
CCTCCAAAAT CACAGCCAGT CTCATTACCC GAGGAACTGA ATCGGGTTCG GTTATCACGG 
CATAAGCTAG AGCGCTGGTG TCACATGCCC TTCTTTGCTA AAACTGTCAC AGGATGTTTT 
GTACGGATTG GCATTGGAAA CCACAATAGC AAGCCAGTTT ACCGGGTGGC TGAGATCACG 
GGTGTTGTGG AAACTGCCAA AGTTTACCAG CTGGGTGGCA CCAGAACAAA TAAAGGGCTC 
CAGCTACGGC ATGGCAATGA CCAGCGAGTG TTCCGCCTAG AGTTTGTGTC CAACCAAGAA 
TTCACTGAAA GTGAATTCAT GAAGTGGAAA GAAGCGATGT TTTCTGCTGG CATGCAGTTG 
CCCACACTAG ATGAAATCAA TAAAAAGGAA TTATCTATTA AGGAAGCTCT CAATTATAAA 
TTCAATGATC AGGACATTGA GGAGATTGTA AAAGAGAAGG AAAGGTTCAG AAAAGCCCCA 
CCAAACTATG CTATGAAGAA GACTCAGCTG CTAAAGGAGA AGGCTATGGC TGAGGACCTG 
GGAGATCAGG ACAAAGCCAA ACAAATCCAA GACCAGCTAA ATGAGCTAGA GGAGCGAGCA 
GAGGCCCTGG ACCGCCAGCG GACCAAGAAC ATATCTGCTA TCAGCTACAT CAACCAGCGG 
AATCGGGAGT GGAATATTGT GGAGTCTGAG AAGGCTCTGG TGGCTGAAAG TCACAACATG 
AGAAACCAAC AGATGGACCC CTTCACCAGG CGGCAGTGCA AACCCACCAT CGTCTCTAAT 
TCCAGGGATC CAGCTGTTCA GGCTGCCATT TTGGCCCAGC TCAATGCAAA ATACGGTTCT 
GGGGTATTAC CAGATGCTCC AAAGGAAATG AGCAAGGGTC AAGGAAAGGA TAAAGATTTG 
AATTCTAAGA CAGCCAGTGA CCTCTCGGAA GACCTGTTCA AAGTGCATGA CTTTGACGTG 
AAGATTGACT TACAGGTTCC AAGCTCAGAG TCAAAAGCTT TGGCCATTAC CTCCAAAGCC 
CCACCAGCCA AGGACGGAGC TCCGAGGAGA TCTCTGAACT TAGAAGACTA CAAAAAACGA 
CGGGGGCTTA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Nature cell biology18(11)1127-1138(2016 Nov)
Strikoudis A,Lazaris C,Trimarchi T,Galvao Neto AL,Yang Y,Ntziachristos P,Rothbart S,Buckley S,Dolgalev I,Stadtfeld M,Strahl BD,Dynlacht BD,Tsirigos A,Aifantis I

CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.
Developmental biology383(1)15-27(2013 Nov)
Zhang K,Haversat JM,Mager J

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.
Cell stem cell4(5)403-15(2009 May)
Ding L,Paszkowski-Rogacz M,Nitzsche A,Slabicki MM,Heninger AK,de Vries I,Kittler R,Junqueira M,Shevchenko A,Schulz H,Hubner N,Doss MX,Sachinidis A,Hescheler J,Iacone R,Anastassiadis K,Stewart AF,Pisabarro MT,Caldarelli A,Poser I,Theis M,Buchholz F

Structure and DNA binding of the human Rtf1 Plus3 domain.
Structure (London, England : 1993)16(1)149-59(2008 Jan)
de Jong RN,Truffault V,Diercks T,Ab E,Daniels MA,Kaptein R,Folkers GE

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

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Rtf1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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