Col20a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col20a1
Entrez Gene ID	73368
Full Name	collagen, type XX, alpha 1
Synonyms	1700051I12Rik
General protein information	Preferred Names collagen, type XX, alpha 1 Names collagen alpha-1(XX) chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H4

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319287.1	XP_017174776.1	collagen alpha-1(XX) chain isoform X1
XM_017319288.1	XP_017174777.1	collagen alpha-1(XX) chain isoform X2
NM_028518.1	NP_082794.1	collagen alpha-1(XX) chain precursor
XM_006500727.3	XP_006500790.1	collagen alpha-1(XX) chain isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col20a1	XP_006235874.1
Homo sapiens (human)	COL20A1	NP_065933.2
Pan troglodytes (chimpanzee)	COL20A1	XP_001148451.1
Bos taurus (cattle)	COL20A1	XP_002692297.2
Mus musculus (house mouse)	Col20a1	NP_082794.1
Gallus gallus (chicken)	COL20A1	NP_001004392.1
Canis lupus familiaris (dog)	COL20A1	XP_543096.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

The Vertebrate Genome Annotation browser 10 years on.
Harrow JL, Steward CA, Frankish A, Gilbert JG, Gonzalez JM, Loveland JE, Mudge J, Sheppard D, Thomas M, Trevanion S, Wilming LG
Nucleic acids research42(Database issue)D771-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Col20a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col20a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31240	XM_017319288.1 Latest version!	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu31240	XM_006500727.3 Latest version!	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu05721	NM_028518.1 Latest version!	Mus musculus collagen, type XX, alpha 1 (Col20a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu31239	XM_017319287.1 Latest version!	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col20a1 ( XM_017319288.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174777.1 Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31240
Clone ID Related Accession (Same CDS sequence)	XM_017319288.1 , XM_006500727.3
Accession Version	XM_017319288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTG GAAACCTGAC CTCCAGCACA GAATACTTGG TCTCTGTGCT TCCTGTCTAT 
GAGAGTGGGG TTGGCAAGAG CCTGCAAGGC CGGGCAACCA CAGCACCCCT GCCTCCACCT 
GGGCCACTGA CCCTAGCTGC AGTGACACCC AGAACTCTCC ACGTCACCTG GCCGCCCTCT 
GCTGGGGTCA CTCAGTACTT GGTGCAGTAC TTGCTGGCTA CCTCCACTGG GGAGGAGCAA 
AAGAGAGAGG TACATGTGGG ACAGCCTGAG GTACTGCTGG ATGGTCTGGA GCCTGGCCAG 
GACTATGATG TCTCAGTGCA GAGCCTTCGG GGACCAGAGG CCAGCGAGGT CCAGAGCATC 
CGTGCCAGGA CCTCTGCCCT GGGGCCCCCA AGACACCTGA CTTTCTCAGA TGTTCGCTAC 
AACTCAACCT GTGTGTCCTG GGAAGCCCAG AGGCCTGTGC GCCTGGTCAA GGTCAGCTAC 
ATCTCTAGTG ATGGCAGCCA CTCTGGGCAG ACTCAGGTTC CTGGGAACCT TACTTCAGCT 
ACTCTGGGCC CACTTTCTTC CTCTACCATG TACACCGTCA GAGTCACCTG TTTTTACCTT 
GGGGGTGGTT CCTCCGTGCT GACTGGCCAT GTAACCACAC GTGAGTCTGC GAAGTCAAGG 
CTGGGGTGCA GTTGGACTGT GCCCCTATGT CCTACTGAAG GGCCTATGTT GAGTGTCTGT 
TCTAGCTGTA TTAGGGCAGG TGGGGACAAG TGTCTGGACA AGGGGCCAGG GACATTCCTC 
CATGTGGCCG ACCCTTGTCT AAAAGTAAAC GCTAAGGTCA GCACTCTATG CTCCATTTAT 
ACACCCCATC TTCTAGAGAA AGCTCCTAGC CCTGGCCAGC TGTCTGTGAT GGAGCTCCCG 
GGAGATGCAG TAAAGCTGTC ATGGCTGGCC ACTGCCTTGT CTGGTGTACT TGTTTATCAG 
ATCAAGTGGA TGCCCTTGGG AGAAGGGAAG GCCCGTGAGA TCTCTGTCCC AGGGACCCTT 
GGCACAGCCA CCTTGCCTGG CCTGATGAAA CATGTGGAAT ATGAGATCAC CATCCTTGCC 
TACTACAGGG ACGGCACTCG CAGCGACCCC GTGTCCCTCC GCTACACCCC CTTGTTGAAT 
TCCACATTGC CTTGCCACTG GATAGCTGCA GCAAGCAGAA GCCCCCCATC CAGCCTGGCC 
TTGTCCTCGG AGACCCCCAA CAGCCTGCAG GTCAACTGGA CACCACCAAG TGGCCATGTC 
CTCCATTACC GGCTCAACTA CACACTGGCC TCAGGCTCTG GACCAGAGAA ATCGATCTCT 
GTACCAGGCA CCAGGAGCCA TGCCGTGCTT CGTGATCTGA TGTCTGCCAC GAAGTACAGG 
GTCCTGGTCT CAGCTGTCTA CAGAGCAGGG GAGAGCATGG CAGTGTCTGC CACATACCGC 
ACAGCAGCCT GCCCAGCCCT GCACCCGGAC AGTTCCCTCT CAGGGTTTGA CCTGATGGTG 
GCCTTTGGCC TCGTGGCAAA GGAGTATGCC TCTATTCGAG GAGTTGCCAT GGAGCCCTCA 
GCCTTGGGTG TGGTCCCAAC CTTCACTCTC TTCAAGGATG CTCAACTGAT GCGTCGGGTC 
AGTGACATCT ACCCAGCAAC CCTGCCACCA GAACACACCA TTGTCTTCCT TGTGCGTCTA 
CTCCCTGAGA CACCCCGGGA AGCCTTTGCA CTGTGGCAGA TGATGGCTGA AGATTTCCAG 
CCCATCCTCG GGGTTCTGCT GGATGCTGGC AGAAAGTCTC TGACTTACTT CAACCATGAT 
TCCCGGGCAG CCTTGCAGGA GGTCACCTTT GACCTGCAGG ATGCAAAGAA GATCTTCTTT 
GGAAGCTTCC ACAAGGTACA TATAGCTGTA GGCCACTCTA AGGTCAGACT CTATGTGGAC 
TGTCGGAAGG TGGCCGAGCG TCCCATTGGG GATGCTGGCA GCCCACCCAC AGGTGGTTTC 
ATCACACTAG GGAGGCTAGC CAAGGCCAGA GGGCCACGGA GCAGCTCAGC CACATTCCAG 
CTCCAGATGT TGCAAATCGT GTGCAGTGAT ACCTGGGCAG ACAAGGACAG GTGTTGTGAG 
ATTCCTGCAC TGAGGGATGG AGAGACGTGC CCGGCCTTCC CTTCAGCCTG TGCCTACTCA 
TCTGAGACCC CTGGGCCACC AGGGCCCCAA GGCCCTCCGG GTCTCCCTGG AAGGAACGGC 
CCTCCAGGAC AGCAGGGGCA CCCAGGGCCC AAGGGGGAGC CGGGCCCACC TGGACAAACA 
GGACCTGAAG GCCCTGGAGG TCAGCAGGGG TCACCAGGGA CCCAGGGCCG CGCAGTCCAG 
GGACCCATGG GGCCACCAGG AGCCAAAGGA GAGAAGGGGG ATCAGGGACT TTCAGGCTTG 
CAGGGCCTCT CTGGCCAGCA AGGCATCCCT GGGAAAACTG GCCTACAGGG ACCAAAGGGC 
ATGAGAGGAC TGGAGGGACC TGCTGGCCTG CCAGGACCAC CTGGCCCCAG GGGGTTCCAG 
GGCTTGGCAG GGGCCAGGGG CACCAATGGG GAGCGAGGAG CCCCAGGAGC TGTGGGTCCC 
ACAGGTCTAC CAGGGTCCAA AGGTGAACGA GGAGAGAAGG GAGAGCCACA GTCCCTTGCC 
ACCATCTTCC AGCTGGTGAG CCAGGCCTGC GAGTCAGCCA TACGGACACA TGTGCTGAAG 
CTGAATTCCT TCCTCCATGA GAATGCCCGT CCCCCAATGC CCTTCATGGC AGAGACAGCT 
AAGCTAGGCA GACCCAGATC TATAGATCCT GGATTACACA ACGAGGCTCT ACTTCCTGGA 
GACTGGGGAC ATATCCTCCG CCCTGAGGAC CAAGGTGAGC CTGTGACTAT CAGCCACACA 
AGCAACCCTA GACTGCAGGA GGTGCAGACC CCAGAATCCC TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174777.1
CDS	1294..4221
Translation	MELGNLTSSTEYLVSVLPVYESGVGKSLQGRATTAPLPPPGPLTLAAVTPRTLHVTWPPSAGVTQYLVQYLLATSTGEEQKREVHVGQPEVLLDGLEPGQDYDVSVQSLRGPEASEVQSIRARTSALGPPRHLTFSDVRYNSTCVSWEAQRPVRLVKVSYISSDGSHSGQTQVPGNLTSATLGPLSSSTMYTVRVTCFYLGGGSSVLTGHVTTRESAKSRLGCSWTVPLCPTEGPMLSVCSSCIRAGGDKCLDKGPGTFLHVADPCLKVNAKVSTLCSIYTPHLLEKAPSPGQLSVMELPGDAVKLSWLATALSGVLVYQIKWMPLGEGKAREISVPGTLGTATLPGLMKHVEYEITILAYYRDGTRSDPVSLRYTPLLNSTLPCHWIAAASRSPPSSLALSSETPNSLQVNWTPPSGHVLHYRLNYTLASGSGPEKSISVPGTRSHAVLRDLMSATKYRVLVSAVYRAGESMAVSATYRTAACPALHPDSSLSGFDLMVAFGLVAKEYASIRGVAMEPSALGVVPTFTLFKDAQLMRRVSDIYPATLPPEHTIVFLVRLLPETPREAFALWQMMAEDFQPILGVLLDAGRKSLTYFNHDSRAALQEVTFDLQDAKKIFFGSFHKVHIAVGHSKVRLYVDCRKVAERPIGDAGSPPTGGFITLGRLAKARGPRSSSATFQLQMLQIVCSDTWADKDRCCEIPALRDGETCPAFPSACAYSSETPGPPGPQGPPGLPGRNGPPGQQGHPGPKGEPGPPGQTGPEGPGGQQGSPGTQGRAVQGPMGPPGAKGEKGDQGLSGLQGLSGQQGIPGKTGLQGPKGMRGLEGPAGLPGPPGPRGFQGLAGARGTNGERGAPGAVGPTGLPGSKGERGEKGEPQSLATIFQLVSQACESAIRTHVLKLNSFLHENARPPMPFMAETAKLGRPRSIDPGLHNEALLPGDWGHILRPEDQGEPVTISHTSNPRLQEVQTPESLE

Target ORF information:

RefSeq Version	XM_017319288.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319288.1

ORF Insert Sequence:

ATGGAGCTTG GAAACCTGAC CTCCAGCACA GAATACTTGG TCTCTGTGCT TCCTGTCTAT 
GAGAGTGGGG TTGGCAAGAG CCTGCAAGGC CGGGCAACCA CAGCACCCCT GCCTCCACCT 
GGGCCACTGA CCCTAGCTGC AGTGACACCC AGAACTCTCC ACGTCACCTG GCCGCCCTCT 
GCTGGGGTCA CTCAGTACTT GGTGCAGTAC TTGCTGGCTA CCTCCACTGG GGAGGAGCAA 
AAGAGAGAGG TACATGTGGG ACAGCCTGAG GTACTGCTGG ATGGTCTGGA GCCTGGCCAG 
GACTATGATG TCTCAGTGCA GAGCCTTCGG GGACCAGAGG CCAGCGAGGT CCAGAGCATC 
CGTGCCAGGA CCTCTGCCCT GGGGCCCCCA AGACACCTGA CTTTCTCAGA TGTTCGCTAC 
AACTCAACCT GTGTGTCCTG GGAAGCCCAG AGGCCTGTGC GCCTGGTCAA GGTCAGCTAC 
ATCTCTAGTG ATGGCAGCCA CTCTGGGCAG ACTCAGGTTC CTGGGAACCT TACTTCAGCT 
ACTCTGGGCC CACTTTCTTC CTCTACCATG TACACCGTCA GAGTCACCTG TTTTTACCTT 
GGGGGTGGTT CCTCCGTGCT GACTGGCCAT GTAACCACAC GTGAGTCTGC GAAGTCAAGG 
CTGGGGTGCA GTTGGACTGT GCCCCTATGT CCTACTGAAG GGCCTATGTT GAGTGTCTGT 
TCTAGCTGTA TTAGGGCAGG TGGGGACAAG TGTCTGGACA AGGGGCCAGG GACATTCCTC 
CATGTGGCCG ACCCTTGTCT AAAAGTAAAC GCTAAGGTCA GCACTCTATG CTCCATTTAT 
ACACCCCATC TTCTAGAGAA AGCTCCTAGC CCTGGCCAGC TGTCTGTGAT GGAGCTCCCG 
GGAGATGCAG TAAAGCTGTC ATGGCTGGCC ACTGCCTTGT CTGGTGTACT TGTTTATCAG 
ATCAAGTGGA TGCCCTTGGG AGAAGGGAAG GCCCGTGAGA TCTCTGTCCC AGGGACCCTT 
GGCACAGCCA CCTTGCCTGG CCTGATGAAA CATGTGGAAT ATGAGATCAC CATCCTTGCC 
TACTACAGGG ACGGCACTCG CAGCGACCCC GTGTCCCTCC GCTACACCCC CTTGTTGAAT 
TCCACATTGC CTTGCCACTG GATAGCTGCA GCAAGCAGAA GCCCCCCATC CAGCCTGGCC 
TTGTCCTCGG AGACCCCCAA CAGCCTGCAG GTCAACTGGA CACCACCAAG TGGCCATGTC 
CTCCATTACC GGCTCAACTA CACACTGGCC TCAGGCTCTG GACCAGAGAA ATCGATCTCT 
GTACCAGGCA CCAGGAGCCA TGCCGTGCTT CGTGATCTGA TGTCTGCCAC GAAGTACAGG 
GTCCTGGTCT CAGCTGTCTA CAGAGCAGGG GAGAGCATGG CAGTGTCTGC CACATACCGC 
ACAGCAGCCT GCCCAGCCCT GCACCCGGAC AGTTCCCTCT CAGGGTTTGA CCTGATGGTG 
GCCTTTGGCC TCGTGGCAAA GGAGTATGCC TCTATTCGAG GAGTTGCCAT GGAGCCCTCA 
GCCTTGGGTG TGGTCCCAAC CTTCACTCTC TTCAAGGATG CTCAACTGAT GCGTCGGGTC 
AGTGACATCT ACCCAGCAAC CCTGCCACCA GAACACACCA TTGTCTTCCT TGTGCGTCTA 
CTCCCTGAGA CACCCCGGGA AGCCTTTGCA CTGTGGCAGA TGATGGCTGA AGATTTCCAG 
CCCATCCTCG GGGTTCTGCT GGATGCTGGC AGAAAGTCTC TGACTTACTT CAACCATGAT 
TCCCGGGCAG CCTTGCAGGA GGTCACCTTT GACCTGCAGG ATGCAAAGAA GATCTTCTTT 
GGAAGCTTCC ACAAGGTACA TATAGCTGTA GGCCACTCTA AGGTCAGACT CTATGTGGAC 
TGTCGGAAGG TGGCCGAGCG TCCCATTGGG GATGCTGGCA GCCCACCCAC AGGTGGTTTC 
ATCACACTAG GGAGGCTAGC CAAGGCCAGA GGGCCACGGA GCAGCTCAGC CACATTCCAG 
CTCCAGATGT TGCAAATCGT GTGCAGTGAT ACCTGGGCAG ACAAGGACAG GTGTTGTGAG 
ATTCCTGCAC TGAGGGATGG AGAGACGTGC CCGGCCTTCC CTTCAGCCTG TGCCTACTCA 
TCTGAGACCC CTGGGCCACC AGGGCCCCAA GGCCCTCCGG GTCTCCCTGG AAGGAACGGC 
CCTCCAGGAC AGCAGGGGCA CCCAGGGCCC AAGGGGGAGC CGGGCCCACC TGGACAAACA 
GGACCTGAAG GCCCTGGAGG TCAGCAGGGG TCACCAGGGA CCCAGGGCCG CGCAGTCCAG 
GGACCCATGG GGCCACCAGG AGCCAAAGGA GAGAAGGGGG ATCAGGGACT TTCAGGCTTG 
CAGGGCCTCT CTGGCCAGCA AGGCATCCCT GGGAAAACTG GCCTACAGGG ACCAAAGGGC 
ATGAGAGGAC TGGAGGGACC TGCTGGCCTG CCAGGACCAC CTGGCCCCAG GGGGTTCCAG 
GGCTTGGCAG GGGCCAGGGG CACCAATGGG GAGCGAGGAG CCCCAGGAGC TGTGGGTCCC 
ACAGGTCTAC CAGGGTCCAA AGGTGAACGA GGAGAGAAGG GAGAGCCACA GTCCCTTGCC 
ACCATCTTCC AGCTGGTGAG CCAGGCCTGC GAGTCAGCCA TACGGACACA TGTGCTGAAG 
CTGAATTCCT TCCTCCATGA GAATGCCCGT CCCCCAATGC CCTTCATGGC AGAGACAGCT 
AAGCTAGGCA GACCCAGATC TATAGATCCT GGATTACACA ACGAGGCTCT ACTTCCTGGA 
GACTGGGGAC ATATCCTCCG CCCTGAGGAC CAAGGTGAGC CTGTGACTAT CAGCCACACA 
AGCAACCCTA GACTGCAGGA GGTGCAGACC CCAGAATCCC TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col20a1 ( XM_006500727.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500790.1 Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu31240
Clone ID Related Accession (Same CDS sequence)	XM_017319288.1 , XM_006500727.3
Accession Version	XM_006500727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTTG GAAACCTGAC CTCCAGCACA GAATACTTGG TCTCTGTGCT TCCTGTCTAT 
GAGAGTGGGG TTGGCAAGAG CCTGCAAGGC CGGGCAACCA CAGCACCCCT GCCTCCACCT 
GGGCCACTGA CCCTAGCTGC AGTGACACCC AGAACTCTCC ACGTCACCTG GCCGCCCTCT 
GCTGGGGTCA CTCAGTACTT GGTGCAGTAC TTGCTGGCTA CCTCCACTGG GGAGGAGCAA 
AAGAGAGAGG TACATGTGGG ACAGCCTGAG GTACTGCTGG ATGGTCTGGA GCCTGGCCAG 
GACTATGATG TCTCAGTGCA GAGCCTTCGG GGACCAGAGG CCAGCGAGGT CCAGAGCATC 
CGTGCCAGGA CCTCTGCCCT GGGGCCCCCA AGACACCTGA CTTTCTCAGA TGTTCGCTAC 
AACTCAACCT GTGTGTCCTG GGAAGCCCAG AGGCCTGTGC GCCTGGTCAA GGTCAGCTAC 
ATCTCTAGTG ATGGCAGCCA CTCTGGGCAG ACTCAGGTTC CTGGGAACCT TACTTCAGCT 
ACTCTGGGCC CACTTTCTTC CTCTACCATG TACACCGTCA GAGTCACCTG TTTTTACCTT 
GGGGGTGGTT CCTCCGTGCT GACTGGCCAT GTAACCACAC GTGAGTCTGC GAAGTCAAGG 
CTGGGGTGCA GTTGGACTGT GCCCCTATGT CCTACTGAAG GGCCTATGTT GAGTGTCTGT 
TCTAGCTGTA TTAGGGCAGG TGGGGACAAG TGTCTGGACA AGGGGCCAGG GACATTCCTC 
CATGTGGCCG ACCCTTGTCT AAAAGTAAAC GCTAAGGTCA GCACTCTATG CTCCATTTAT 
ACACCCCATC TTCTAGAGAA AGCTCCTAGC CCTGGCCAGC TGTCTGTGAT GGAGCTCCCG 
GGAGATGCAG TAAAGCTGTC ATGGCTGGCC ACTGCCTTGT CTGGTGTACT TGTTTATCAG 
ATCAAGTGGA TGCCCTTGGG AGAAGGGAAG GCCCGTGAGA TCTCTGTCCC AGGGACCCTT 
GGCACAGCCA CCTTGCCTGG CCTGATGAAA CATGTGGAAT ATGAGATCAC CATCCTTGCC 
TACTACAGGG ACGGCACTCG CAGCGACCCC GTGTCCCTCC GCTACACCCC CTTGTTGAAT 
TCCACATTGC CTTGCCACTG GATAGCTGCA GCAAGCAGAA GCCCCCCATC CAGCCTGGCC 
TTGTCCTCGG AGACCCCCAA CAGCCTGCAG GTCAACTGGA CACCACCAAG TGGCCATGTC 
CTCCATTACC GGCTCAACTA CACACTGGCC TCAGGCTCTG GACCAGAGAA ATCGATCTCT 
GTACCAGGCA CCAGGAGCCA TGCCGTGCTT CGTGATCTGA TGTCTGCCAC GAAGTACAGG 
GTCCTGGTCT CAGCTGTCTA CAGAGCAGGG GAGAGCATGG CAGTGTCTGC CACATACCGC 
ACAGCAGCCT GCCCAGCCCT GCACCCGGAC AGTTCCCTCT CAGGGTTTGA CCTGATGGTG 
GCCTTTGGCC TCGTGGCAAA GGAGTATGCC TCTATTCGAG GAGTTGCCAT GGAGCCCTCA 
GCCTTGGGTG TGGTCCCAAC CTTCACTCTC TTCAAGGATG CTCAACTGAT GCGTCGGGTC 
AGTGACATCT ACCCAGCAAC CCTGCCACCA GAACACACCA TTGTCTTCCT TGTGCGTCTA 
CTCCCTGAGA CACCCCGGGA AGCCTTTGCA CTGTGGCAGA TGATGGCTGA AGATTTCCAG 
CCCATCCTCG GGGTTCTGCT GGATGCTGGC AGAAAGTCTC TGACTTACTT CAACCATGAT 
TCCCGGGCAG CCTTGCAGGA GGTCACCTTT GACCTGCAGG ATGCAAAGAA GATCTTCTTT 
GGAAGCTTCC ACAAGGTACA TATAGCTGTA GGCCACTCTA AGGTCAGACT CTATGTGGAC 
TGTCGGAAGG TGGCCGAGCG TCCCATTGGG GATGCTGGCA GCCCACCCAC AGGTGGTTTC 
ATCACACTAG GGAGGCTAGC CAAGGCCAGA GGGCCACGGA GCAGCTCAGC CACATTCCAG 
CTCCAGATGT TGCAAATCGT GTGCAGTGAT ACCTGGGCAG ACAAGGACAG GTGTTGTGAG 
ATTCCTGCAC TGAGGGATGG AGAGACGTGC CCGGCCTTCC CTTCAGCCTG TGCCTACTCA 
TCTGAGACCC CTGGGCCACC AGGGCCCCAA GGCCCTCCGG GTCTCCCTGG AAGGAACGGC 
CCTCCAGGAC AGCAGGGGCA CCCAGGGCCC AAGGGGGAGC CGGGCCCACC TGGACAAACA 
GGACCTGAAG GCCCTGGAGG TCAGCAGGGG TCACCAGGGA CCCAGGGCCG CGCAGTCCAG 
GGACCCATGG GGCCACCAGG AGCCAAAGGA GAGAAGGGGG ATCAGGGACT TTCAGGCTTG 
CAGGGCCTCT CTGGCCAGCA AGGCATCCCT GGGAAAACTG GCCTACAGGG ACCAAAGGGC 
ATGAGAGGAC TGGAGGGACC TGCTGGCCTG CCAGGACCAC CTGGCCCCAG GGGGTTCCAG 
GGCTTGGCAG GGGCCAGGGG CACCAATGGG GAGCGAGGAG CCCCAGGAGC TGTGGGTCCC 
ACAGGTCTAC CAGGGTCCAA AGGTGAACGA GGAGAGAAGG GAGAGCCACA GTCCCTTGCC 
ACCATCTTCC AGCTGGTGAG CCAGGCCTGC GAGTCAGCCA TACGGACACA TGTGCTGAAG 
CTGAATTCCT TCCTCCATGA GAATGCCCGT CCCCCAATGC CCTTCATGGC AGAGACAGCT 
AAGCTAGGCA GACCCAGATC TATAGATCCT GGATTACACA ACGAGGCTCT ACTTCCTGGA 
GACTGGGGAC ATATCCTCCG CCCTGAGGAC CAAGGTGAGC CTGTGACTAT CAGCCACACA 
AGCAACCCTA GACTGCAGGA GGTGCAGACC CCAGAATCCC TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500790.1
CDS	725..3652
Translation	MELGNLTSSTEYLVSVLPVYESGVGKSLQGRATTAPLPPPGPLTLAAVTPRTLHVTWPPSAGVTQYLVQYLLATSTGEEQKREVHVGQPEVLLDGLEPGQDYDVSVQSLRGPEASEVQSIRARTSALGPPRHLTFSDVRYNSTCVSWEAQRPVRLVKVSYISSDGSHSGQTQVPGNLTSATLGPLSSSTMYTVRVTCFYLGGGSSVLTGHVTTRESAKSRLGCSWTVPLCPTEGPMLSVCSSCIRAGGDKCLDKGPGTFLHVADPCLKVNAKVSTLCSIYTPHLLEKAPSPGQLSVMELPGDAVKLSWLATALSGVLVYQIKWMPLGEGKAREISVPGTLGTATLPGLMKHVEYEITILAYYRDGTRSDPVSLRYTPLLNSTLPCHWIAAASRSPPSSLALSSETPNSLQVNWTPPSGHVLHYRLNYTLASGSGPEKSISVPGTRSHAVLRDLMSATKYRVLVSAVYRAGESMAVSATYRTAACPALHPDSSLSGFDLMVAFGLVAKEYASIRGVAMEPSALGVVPTFTLFKDAQLMRRVSDIYPATLPPEHTIVFLVRLLPETPREAFALWQMMAEDFQPILGVLLDAGRKSLTYFNHDSRAALQEVTFDLQDAKKIFFGSFHKVHIAVGHSKVRLYVDCRKVAERPIGDAGSPPTGGFITLGRLAKARGPRSSSATFQLQMLQIVCSDTWADKDRCCEIPALRDGETCPAFPSACAYSSETPGPPGPQGPPGLPGRNGPPGQQGHPGPKGEPGPPGQTGPEGPGGQQGSPGTQGRAVQGPMGPPGAKGEKGDQGLSGLQGLSGQQGIPGKTGLQGPKGMRGLEGPAGLPGPPGPRGFQGLAGARGTNGERGAPGAVGPTGLPGSKGERGEKGEPQSLATIFQLVSQACESAIRTHVLKLNSFLHENARPPMPFMAETAKLGRPRSIDPGLHNEALLPGDWGHILRPEDQGEPVTISHTSNPRLQEVQTPESLE

Target ORF information:

RefSeq Version	XM_006500727.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500727.3

ORF Insert Sequence:

ATGGAGCTTG GAAACCTGAC CTCCAGCACA GAATACTTGG TCTCTGTGCT TCCTGTCTAT 
GAGAGTGGGG TTGGCAAGAG CCTGCAAGGC CGGGCAACCA CAGCACCCCT GCCTCCACCT 
GGGCCACTGA CCCTAGCTGC AGTGACACCC AGAACTCTCC ACGTCACCTG GCCGCCCTCT 
GCTGGGGTCA CTCAGTACTT GGTGCAGTAC TTGCTGGCTA CCTCCACTGG GGAGGAGCAA 
AAGAGAGAGG TACATGTGGG ACAGCCTGAG GTACTGCTGG ATGGTCTGGA GCCTGGCCAG 
GACTATGATG TCTCAGTGCA GAGCCTTCGG GGACCAGAGG CCAGCGAGGT CCAGAGCATC 
CGTGCCAGGA CCTCTGCCCT GGGGCCCCCA AGACACCTGA CTTTCTCAGA TGTTCGCTAC 
AACTCAACCT GTGTGTCCTG GGAAGCCCAG AGGCCTGTGC GCCTGGTCAA GGTCAGCTAC 
ATCTCTAGTG ATGGCAGCCA CTCTGGGCAG ACTCAGGTTC CTGGGAACCT TACTTCAGCT 
ACTCTGGGCC CACTTTCTTC CTCTACCATG TACACCGTCA GAGTCACCTG TTTTTACCTT 
GGGGGTGGTT CCTCCGTGCT GACTGGCCAT GTAACCACAC GTGAGTCTGC GAAGTCAAGG 
CTGGGGTGCA GTTGGACTGT GCCCCTATGT CCTACTGAAG GGCCTATGTT GAGTGTCTGT 
TCTAGCTGTA TTAGGGCAGG TGGGGACAAG TGTCTGGACA AGGGGCCAGG GACATTCCTC 
CATGTGGCCG ACCCTTGTCT AAAAGTAAAC GCTAAGGTCA GCACTCTATG CTCCATTTAT 
ACACCCCATC TTCTAGAGAA AGCTCCTAGC CCTGGCCAGC TGTCTGTGAT GGAGCTCCCG 
GGAGATGCAG TAAAGCTGTC ATGGCTGGCC ACTGCCTTGT CTGGTGTACT TGTTTATCAG 
ATCAAGTGGA TGCCCTTGGG AGAAGGGAAG GCCCGTGAGA TCTCTGTCCC AGGGACCCTT 
GGCACAGCCA CCTTGCCTGG CCTGATGAAA CATGTGGAAT ATGAGATCAC CATCCTTGCC 
TACTACAGGG ACGGCACTCG CAGCGACCCC GTGTCCCTCC GCTACACCCC CTTGTTGAAT 
TCCACATTGC CTTGCCACTG GATAGCTGCA GCAAGCAGAA GCCCCCCATC CAGCCTGGCC 
TTGTCCTCGG AGACCCCCAA CAGCCTGCAG GTCAACTGGA CACCACCAAG TGGCCATGTC 
CTCCATTACC GGCTCAACTA CACACTGGCC TCAGGCTCTG GACCAGAGAA ATCGATCTCT 
GTACCAGGCA CCAGGAGCCA TGCCGTGCTT CGTGATCTGA TGTCTGCCAC GAAGTACAGG 
GTCCTGGTCT CAGCTGTCTA CAGAGCAGGG GAGAGCATGG CAGTGTCTGC CACATACCGC 
ACAGCAGCCT GCCCAGCCCT GCACCCGGAC AGTTCCCTCT CAGGGTTTGA CCTGATGGTG 
GCCTTTGGCC TCGTGGCAAA GGAGTATGCC TCTATTCGAG GAGTTGCCAT GGAGCCCTCA 
GCCTTGGGTG TGGTCCCAAC CTTCACTCTC TTCAAGGATG CTCAACTGAT GCGTCGGGTC 
AGTGACATCT ACCCAGCAAC CCTGCCACCA GAACACACCA TTGTCTTCCT TGTGCGTCTA 
CTCCCTGAGA CACCCCGGGA AGCCTTTGCA CTGTGGCAGA TGATGGCTGA AGATTTCCAG 
CCCATCCTCG GGGTTCTGCT GGATGCTGGC AGAAAGTCTC TGACTTACTT CAACCATGAT 
TCCCGGGCAG CCTTGCAGGA GGTCACCTTT GACCTGCAGG ATGCAAAGAA GATCTTCTTT 
GGAAGCTTCC ACAAGGTACA TATAGCTGTA GGCCACTCTA AGGTCAGACT CTATGTGGAC 
TGTCGGAAGG TGGCCGAGCG TCCCATTGGG GATGCTGGCA GCCCACCCAC AGGTGGTTTC 
ATCACACTAG GGAGGCTAGC CAAGGCCAGA GGGCCACGGA GCAGCTCAGC CACATTCCAG 
CTCCAGATGT TGCAAATCGT GTGCAGTGAT ACCTGGGCAG ACAAGGACAG GTGTTGTGAG 
ATTCCTGCAC TGAGGGATGG AGAGACGTGC CCGGCCTTCC CTTCAGCCTG TGCCTACTCA 
TCTGAGACCC CTGGGCCACC AGGGCCCCAA GGCCCTCCGG GTCTCCCTGG AAGGAACGGC 
CCTCCAGGAC AGCAGGGGCA CCCAGGGCCC AAGGGGGAGC CGGGCCCACC TGGACAAACA 
GGACCTGAAG GCCCTGGAGG TCAGCAGGGG TCACCAGGGA CCCAGGGCCG CGCAGTCCAG 
GGACCCATGG GGCCACCAGG AGCCAAAGGA GAGAAGGGGG ATCAGGGACT TTCAGGCTTG 
CAGGGCCTCT CTGGCCAGCA AGGCATCCCT GGGAAAACTG GCCTACAGGG ACCAAAGGGC 
ATGAGAGGAC TGGAGGGACC TGCTGGCCTG CCAGGACCAC CTGGCCCCAG GGGGTTCCAG 
GGCTTGGCAG GGGCCAGGGG CACCAATGGG GAGCGAGGAG CCCCAGGAGC TGTGGGTCCC 
ACAGGTCTAC CAGGGTCCAA AGGTGAACGA GGAGAGAAGG GAGAGCCACA GTCCCTTGCC 
ACCATCTTCC AGCTGGTGAG CCAGGCCTGC GAGTCAGCCA TACGGACACA TGTGCTGAAG 
CTGAATTCCT TCCTCCATGA GAATGCCCGT CCCCCAATGC CCTTCATGGC AGAGACAGCT 
AAGCTAGGCA GACCCAGATC TATAGATCCT GGATTACACA ACGAGGCTCT ACTTCCTGGA 
GACTGGGGAC ATATCCTCCG CCCTGAGGAC CAAGGTGAGC CTGTGACTAT CAGCCACACA 
AGCAACCCTA GACTGCAGGA GGTGCAGACC CCAGAATCCC TGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col20a1 ( NM_028518.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082794.1 Mus musculus collagen, type XX, alpha 1 (Col20a1), mRNA.

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CloneID	OMu05721
Clone ID Related Accession (Same CDS sequence)	NM_028518.1
Accession Version	NM_028518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XX) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649560.7 and AL450341.10. On or before Sep 20, 2009 this sequence version replaced XM_914804.1, XM_001473208.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTCC AAGGCTCCTA CCAACACTTC TGCCTCTGGA TGTTCTTGGG CACCACGTTG 
GCACTGGGGC AGGGACAAGT AAGTAGCCGC CTGAGGCTGG CAGTACTGCC TGAGGACCAG 
CTGCAGATGA AGTGGAGGGA AGCAGAGGGA AGTGGCCTTG GCTTCTTGGT GCAGGTGACA 
CCTATGGCAG GGGACTTGGA GCAGGAGCTG ATACTGACCA CCAAGACCCC CAAGGCCACT 
GTGGGTGGCC TGAACCCTTC CAAGAGCTAC ACCTTGCAGA TCTTTGAGCT CACTGACTCG 
GGGCCTATCC TGCTGGCACG AAGGGAGTTT GTGATTGAGG ATCTGAAGAG CCAATCTTTG 
GGCAGAGGCA GCCGGAGGCT GGCTGGAGCC ACCCTGGAGC CTACCTCCTT GCCTCTGAGG 
GGCCCAGACT CTGAGAAAAC ATCTGAGCCC AGCATTGCCT TTACCTTAAG CAGGGACCTG 
CCCATTCTTG ATCACCCCCA GTTCCAATGT ACACCCCCAA CACCTGCTGA TATAATCTTC 
CTGGTGGACG GGTCCTGGAG CATCGGCCAC AACCACTTCC AGCAGGTGAA AGACTTCCTG 
GCCAGCATCA TCACACAATT TGCAATAGGA CCAGATAAAG TCCAAGTAGG TCTGACACAG 
TACAGTGGAG ACCCCCAGAC CGAGTGGGAC CTGAACTCCT TCCAAACCAA GGAGCAAGTG 
CTAGCAGCTG TACACCACCT CCGCTACAAG GGAGGAAACA CATTCACAGG CCTTGCTCTA 
ACCCATGTGC TGGAGCAGAA CCTGAAGCCA GCAGCCGGTG TCCGTCCAGA GGCAGCCAAA 
GTGTTGATTC TGGTGACAGA TGGCAAGTCC CAGGATGATG TGCGCACAGC TGCCAGGATC 
CTTAAGGATC AGGACATTGA TGTCTTTGTT GTGGGTGTGA AGAATGTCGA TGAGGCTGAG 
CTGAAGCTCC TGGCGTCCCA GCCACTGGAC ATCACTGTCC ACAACGTGCT GGACTTCCCT 
CAGCTGGACA CCCTAGCTCC TCTGCTCAGC CGACTCATTT GCCAAAAAAT TCAGGGCAGA 
GGCCCAGTCA AACCAGCAGC AGGTACTCGG GTCCTGGACC CCCTCCCCAC GCCCACCAGA 
CTGATCCTGA CCCACGCCAC CTCATCCAGT ATCCACCTGT CTTGGACTCC AGCCTTATAC 
CCACCCCTCA AGTATCTAAT TGTATGGCAG CCTTCTAGGG GTGGTGCCCC CAAGGAGGTG 
GTGGTGGAGG GGCCTGTCTC ATCCATGGAG CTTGGAAACC TGACCTCCAG CACAGAATAC 
TTGGTCTCTG TGCTTCCTGT CTATGAGAGT GGGGTTGGCA AGAGCCTGCA AGGCCGGGCA 
ACCACAGCAC CCCTGCCTCC ACCTGGGCCA CTGACCCTAG CTGCAGTGAC ACCCAGAACT 
CTCCACGTCA CCTGGCCGCC CTCTGCTGGG GTCACTCAGT ACTTGGTGCA GTACTTGCTG 
GCTACCTCCA CTGGGGAGGA GCAAAAGAGA GAGGTACATG TGGGACAGCC TGAGGTACTG 
CTGGATGGTC TGGAGCCTGG CCAGGACTAT GATGTCTCAG TGCAGAGCCT TCGGGGACCA 
GAGGCCAGCG AGGTCCAGAG CATCCGTGCC AGGACCTCTG CCCTGGGGCC CCCAAGACAC 
CTGACTTTCT CAGATGTTCG CTACAACTCA ACCTGTGTGT CCTGGGAAGC CCAGAGGCCT 
GTGCGCCTGG TCAAGGTCAG CTACATCTCT AGTGATGGCA GCCACTCTGG GCAGACTCAG 
GTTCCTGGGA ACCTTACTTC AGCTACTCTG GGCCCACTTT CTTCCTCTAC CATGTACACC 
GTCAGAGTCA CCTGTTTTTA CCTTGGGGGT GGTTCCTCCG TGCTGACTGG CCATGTAACC 
ACACAGAAAG CTCCTAGCCC TGGCCAGCTG TCTGTGATGG AGCTCCCGGG AGATGCAGTA 
AAGCTGTCAT GGCTGGCCAC TGCCTTGTCT GGTGTACTTG TTTATCAGAT CAAGTGGATG 
CCCTTGGGAG AAGGGAAGGC CCGTGAGATC TCTGTCCCAG GGACCCTTGG CACAGCCACC 
TTGCCTGGCC TGATGAAACA TGTGGAATAT GAGATCACCA TCCTTGCCTA CTACAGGGAC 
GGCACTCGCA GCGACCCCGT GTCCCTCCGC TACACCCCCT CTGCAGCAAG CAGAAGCCCC 
CCATCCAGCC TGGCCTTGTC CTCGGAGACC CCCAACAGCC TGCAGGTCAA CTGGACACCA 
CCAAGTGGCC ATGTCCTCCA TTACCGGCTC AACTACACAC TGGCCTCAGG CTCTGGACCA 
GAGAAATCGA TCTCTGTACC AGGCACCAGG AGCCATGCCG TGCTTCGTGA TCTGATGTCT 
GCCACGAAGT ACAGGGTCCT GGTCTCAGCT GTCTACAGAG CAGGGGAGAG CATGGCAGTG 
TCTGCCACAT ACCGCACAGC AGCCTGCCCA GCCCTGCACC CGGACAGTTC CCTCTCAGGG 
TTTGACCTGA TGGTGGCCTT TGGCCTCGTG GCAAAGGAGT ATGCCTCTAT TCGAGGAGTT 
GCCATGGAGC CCTCAGCCTT GGGTGTGGTC CCAACCTTCA CTCTCTTCAA GGATGCTCAA 
CTGATGCGTC GGGTCAGTGA CATCTACCCA GCAACCCTGC CACCAGAACA CACCATTGTC 
TTCCTTGTGC GTCTACTCCC TGAGACACCC CGGGAAGCCT TTGCACTGTG GCAGATGATG 
GCTGAAGATT TCCAGCCCAT CCTCGGGGTT CTGCTGGATG CTGGCAGAAA GTCTCTGACT 
TACTTCAACC ATGATTCCCG GGCAGCCTTG CAGGAGGTCA CCTTTGACCT GCAGGATGCA 
AAGAAGATCT TCTTTGGAAG CTTCCACAAG GTACATATAG CTGTAGGCCA CTCTAAGGTC 
AGACTCTATG TGGACTGTCG GAAGGTGGCC GAGCGTCCCA TTGGGGATGC TGGCAGCCCA 
CCCACAGGTG GTTTCATCAC ACTAGGGAGG CTAGCCAAGG CCAGAGGGCC ACGGAGCAGC 
TCAGCCACAT TCCAGCTCCA GATGTTGCAA ATCGTGTGCA GTGATACCTG GGCAGACAAG 
GACAGGTGTT GTGAGATTCC TGCACTGAGG GATGGAGAGA CGTGCCCGGC CTTCCCTTCA 
GCCTGTGCCT ACTCATCTGA GACCCCTGGG CCACCAGGGC CCCAAGGCCC TCCGGGTCTC 
CCTGGAAGGA ACGGCCCTCC AGGACAGCAG GGGCACCCAG GGCCCAAGGG GGAGCCGGGC 
CCACCTGGAC AAACAGGACC TGAAGGCCCT GGAGGTCAGC AGGGGTCACC AGGGACCCAG 
GGCCGCGCAG TCCAGGGACC CATGGGGCCA CCAGGAGCCA AAGGAGAGAA GGGGGATCAG 
GGACTTTCAG GCTTGCAGGG CCTCTCTGGC CAGCAAGGCA TCCCTGGGAA AACTGGCCTA 
CAGGGACCAA AGGGCATGAG AGGACTGGAG GGACCTGCTG GCCTGCCAGG ACCACCTGGC 
CCCAGGGGGT TCCAGGGCTT GGCAGGGGCC AGGGGCACCA ATGGGGAGCG AGGAGCCCCA 
GGAGCTGTGG GTCCCACAGG TCTACCAGGG TCCAAAGGTG AACGAGGAGA GAAGGGAGAG 
CCACAGTCCC TTGCCACCAT CTTCCAGCTG GTGAGCCAGG CCTGCGAGTC AGCCATACGG 
ACACATGTGC TGAAGCTGAA TTCCTTCCTC CATGAGAATG CCCGTCCCCC AATGCCCTTC 
ATGGCAGAGA CAGCTAAGCT AGGCAGACCC AGATCTATAG ATCCTGGATT ACACAACGAG 
GCTCTACTTC CTGGAGACTG GGGACATATC CTCCGCCCTG AGGACCAAGG TGAGCCTGTG 
ACTATCAGCC ACACAAGCAA CCCTAGACTG CAGGAGGTGC AGACCCCAGA ATCCCTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082794.1
CDS	1..3963
Translation	MSLQGSYQHFCLWMFLGTTLALGQGQVSSRLRLAVLPEDQLQMKWREAEGSGLGFLVQVTPMAGDLEQELILTTKTPKATVGGLNPSKSYTLQIFELTDSGPILLARREFVIEDLKSQSLGRGSRRLAGATLEPTSLPLRGPDSEKTSEPSIAFTLSRDLPILDHPQFQCTPPTPADIIFLVDGSWSIGHNHFQQVKDFLASIITQFAIGPDKVQVGLTQYSGDPQTEWDLNSFQTKEQVLAAVHHLRYKGGNTFTGLALTHVLEQNLKPAAGVRPEAAKVLILVTDGKSQDDVRTAARILKDQDIDVFVVGVKNVDEAELKLLASQPLDITVHNVLDFPQLDTLAPLLSRLICQKIQGRGPVKPAAGTRVLDPLPTPTRLILTHATSSSIHLSWTPALYPPLKYLIVWQPSRGGAPKEVVVEGPVSSMELGNLTSSTEYLVSVLPVYESGVGKSLQGRATTAPLPPPGPLTLAAVTPRTLHVTWPPSAGVTQYLVQYLLATSTGEEQKREVHVGQPEVLLDGLEPGQDYDVSVQSLRGPEASEVQSIRARTSALGPPRHLTFSDVRYNSTCVSWEAQRPVRLVKVSYISSDGSHSGQTQVPGNLTSATLGPLSSSTMYTVRVTCFYLGGGSSVLTGHVTTQKAPSPGQLSVMELPGDAVKLSWLATALSGVLVYQIKWMPLGEGKAREISVPGTLGTATLPGLMKHVEYEITILAYYRDGTRSDPVSLRYTPSAASRSPPSSLALSSETPNSLQVNWTPPSGHVLHYRLNYTLASGSGPEKSISVPGTRSHAVLRDLMSATKYRVLVSAVYRAGESMAVSATYRTAACPALHPDSSLSGFDLMVAFGLVAKEYASIRGVAMEPSALGVVPTFTLFKDAQLMRRVSDIYPATLPPEHTIVFLVRLLPETPREAFALWQMMAEDFQPILGVLLDAGRKSLTYFNHDSRAALQEVTFDLQDAKKIFFGSFHKVHIAVGHSKVRLYVDCRKVAERPIGDAGSPPTGGFITLGRLAKARGPRSSSATFQLQMLQIVCSDTWADKDRCCEIPALRDGETCPAFPSACAYSSETPGPPGPQGPPGLPGRNGPPGQQGHPGPKGEPGPPGQTGPEGPGGQQGSPGTQGRAVQGPMGPPGAKGEKGDQGLSGLQGLSGQQGIPGKTGLQGPKGMRGLEGPAGLPGPPGPRGFQGLAGARGTNGERGAPGAVGPTGLPGSKGERGEKGEPQSLATIFQLVSQACESAIRTHVLKLNSFLHENARPPMPFMAETAKLGRPRSIDPGLHNEALLPGDWGHILRPEDQGEPVTISHTSNPRLQEVQTPESLE

Target ORF information:

RefSeq Version	NM_028518.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XX, alpha 1 (Col20a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028518.1

ORF Insert Sequence:

ATGAGCCTCC AAGGCTCCTA CCAACACTTC TGCCTCTGGA TGTTCTTGGG CACCACGTTG 
GCACTGGGGC AGGGACAAGT AAGTAGCCGC CTGAGGCTGG CAGTACTGCC TGAGGACCAG 
CTGCAGATGA AGTGGAGGGA AGCAGAGGGA AGTGGCCTTG GCTTCTTGGT GCAGGTGACA 
CCTATGGCAG GGGACTTGGA GCAGGAGCTG ATACTGACCA CCAAGACCCC CAAGGCCACT 
GTGGGTGGCC TGAACCCTTC CAAGAGCTAC ACCTTGCAGA TCTTTGAGCT CACTGACTCG 
GGGCCTATCC TGCTGGCACG AAGGGAGTTT GTGATTGAGG ATCTGAAGAG CCAATCTTTG 
GGCAGAGGCA GCCGGAGGCT GGCTGGAGCC ACCCTGGAGC CTACCTCCTT GCCTCTGAGG 
GGCCCAGACT CTGAGAAAAC ATCTGAGCCC AGCATTGCCT TTACCTTAAG CAGGGACCTG 
CCCATTCTTG ATCACCCCCA GTTCCAATGT ACACCCCCAA CACCTGCTGA TATAATCTTC 
CTGGTGGACG GGTCCTGGAG CATCGGCCAC AACCACTTCC AGCAGGTGAA AGACTTCCTG 
GCCAGCATCA TCACACAATT TGCAATAGGA CCAGATAAAG TCCAAGTAGG TCTGACACAG 
TACAGTGGAG ACCCCCAGAC CGAGTGGGAC CTGAACTCCT TCCAAACCAA GGAGCAAGTG 
CTAGCAGCTG TACACCACCT CCGCTACAAG GGAGGAAACA CATTCACAGG CCTTGCTCTA 
ACCCATGTGC TGGAGCAGAA CCTGAAGCCA GCAGCCGGTG TCCGTCCAGA GGCAGCCAAA 
GTGTTGATTC TGGTGACAGA TGGCAAGTCC CAGGATGATG TGCGCACAGC TGCCAGGATC 
CTTAAGGATC AGGACATTGA TGTCTTTGTT GTGGGTGTGA AGAATGTCGA TGAGGCTGAG 
CTGAAGCTCC TGGCGTCCCA GCCACTGGAC ATCACTGTCC ACAACGTGCT GGACTTCCCT 
CAGCTGGACA CCCTAGCTCC TCTGCTCAGC CGACTCATTT GCCAAAAAAT TCAGGGCAGA 
GGCCCAGTCA AACCAGCAGC AGGTACTCGG GTCCTGGACC CCCTCCCCAC GCCCACCAGA 
CTGATCCTGA CCCACGCCAC CTCATCCAGT ATCCACCTGT CTTGGACTCC AGCCTTATAC 
CCACCCCTCA AGTATCTAAT TGTATGGCAG CCTTCTAGGG GTGGTGCCCC CAAGGAGGTG 
GTGGTGGAGG GGCCTGTCTC ATCCATGGAG CTTGGAAACC TGACCTCCAG CACAGAATAC 
TTGGTCTCTG TGCTTCCTGT CTATGAGAGT GGGGTTGGCA AGAGCCTGCA AGGCCGGGCA 
ACCACAGCAC CCCTGCCTCC ACCTGGGCCA CTGACCCTAG CTGCAGTGAC ACCCAGAACT 
CTCCACGTCA CCTGGCCGCC CTCTGCTGGG GTCACTCAGT ACTTGGTGCA GTACTTGCTG 
GCTACCTCCA CTGGGGAGGA GCAAAAGAGA GAGGTACATG TGGGACAGCC TGAGGTACTG 
CTGGATGGTC TGGAGCCTGG CCAGGACTAT GATGTCTCAG TGCAGAGCCT TCGGGGACCA 
GAGGCCAGCG AGGTCCAGAG CATCCGTGCC AGGACCTCTG CCCTGGGGCC CCCAAGACAC 
CTGACTTTCT CAGATGTTCG CTACAACTCA ACCTGTGTGT CCTGGGAAGC CCAGAGGCCT 
GTGCGCCTGG TCAAGGTCAG CTACATCTCT AGTGATGGCA GCCACTCTGG GCAGACTCAG 
GTTCCTGGGA ACCTTACTTC AGCTACTCTG GGCCCACTTT CTTCCTCTAC CATGTACACC 
GTCAGAGTCA CCTGTTTTTA CCTTGGGGGT GGTTCCTCCG TGCTGACTGG CCATGTAACC 
ACACAGAAAG CTCCTAGCCC TGGCCAGCTG TCTGTGATGG AGCTCCCGGG AGATGCAGTA 
AAGCTGTCAT GGCTGGCCAC TGCCTTGTCT GGTGTACTTG TTTATCAGAT CAAGTGGATG 
CCCTTGGGAG AAGGGAAGGC CCGTGAGATC TCTGTCCCAG GGACCCTTGG CACAGCCACC 
TTGCCTGGCC TGATGAAACA TGTGGAATAT GAGATCACCA TCCTTGCCTA CTACAGGGAC 
GGCACTCGCA GCGACCCCGT GTCCCTCCGC TACACCCCCT CTGCAGCAAG CAGAAGCCCC 
CCATCCAGCC TGGCCTTGTC CTCGGAGACC CCCAACAGCC TGCAGGTCAA CTGGACACCA 
CCAAGTGGCC ATGTCCTCCA TTACCGGCTC AACTACACAC TGGCCTCAGG CTCTGGACCA 
GAGAAATCGA TCTCTGTACC AGGCACCAGG AGCCATGCCG TGCTTCGTGA TCTGATGTCT 
GCCACGAAGT ACAGGGTCCT GGTCTCAGCT GTCTACAGAG CAGGGGAGAG CATGGCAGTG 
TCTGCCACAT ACCGCACAGC AGCCTGCCCA GCCCTGCACC CGGACAGTTC CCTCTCAGGG 
TTTGACCTGA TGGTGGCCTT TGGCCTCGTG GCAAAGGAGT ATGCCTCTAT TCGAGGAGTT 
GCCATGGAGC CCTCAGCCTT GGGTGTGGTC CCAACCTTCA CTCTCTTCAA GGATGCTCAA 
CTGATGCGTC GGGTCAGTGA CATCTACCCA GCAACCCTGC CACCAGAACA CACCATTGTC 
TTCCTTGTGC GTCTACTCCC TGAGACACCC CGGGAAGCCT TTGCACTGTG GCAGATGATG 
GCTGAAGATT TCCAGCCCAT CCTCGGGGTT CTGCTGGATG CTGGCAGAAA GTCTCTGACT 
TACTTCAACC ATGATTCCCG GGCAGCCTTG CAGGAGGTCA CCTTTGACCT GCAGGATGCA 
AAGAAGATCT TCTTTGGAAG CTTCCACAAG GTACATATAG CTGTAGGCCA CTCTAAGGTC 
AGACTCTATG TGGACTGTCG GAAGGTGGCC GAGCGTCCCA TTGGGGATGC TGGCAGCCCA 
CCCACAGGTG GTTTCATCAC ACTAGGGAGG CTAGCCAAGG CCAGAGGGCC ACGGAGCAGC 
TCAGCCACAT TCCAGCTCCA GATGTTGCAA ATCGTGTGCA GTGATACCTG GGCAGACAAG 
GACAGGTGTT GTGAGATTCC TGCACTGAGG GATGGAGAGA CGTGCCCGGC CTTCCCTTCA 
GCCTGTGCCT ACTCATCTGA GACCCCTGGG CCACCAGGGC CCCAAGGCCC TCCGGGTCTC 
CCTGGAAGGA ACGGCCCTCC AGGACAGCAG GGGCACCCAG GGCCCAAGGG GGAGCCGGGC 
CCACCTGGAC AAACAGGACC TGAAGGCCCT GGAGGTCAGC AGGGGTCACC AGGGACCCAG 
GGCCGCGCAG TCCAGGGACC CATGGGGCCA CCAGGAGCCA AAGGAGAGAA GGGGGATCAG 
GGACTTTCAG GCTTGCAGGG CCTCTCTGGC CAGCAAGGCA TCCCTGGGAA AACTGGCCTA 
CAGGGACCAA AGGGCATGAG AGGACTGGAG GGACCTGCTG GCCTGCCAGG ACCACCTGGC 
CCCAGGGGGT TCCAGGGCTT GGCAGGGGCC AGGGGCACCA ATGGGGAGCG AGGAGCCCCA 
GGAGCTGTGG GTCCCACAGG TCTACCAGGG TCCAAAGGTG AACGAGGAGA GAAGGGAGAG 
CCACAGTCCC TTGCCACCAT CTTCCAGCTG GTGAGCCAGG CCTGCGAGTC AGCCATACGG 
ACACATGTGC TGAAGCTGAA TTCCTTCCTC CATGAGAATG CCCGTCCCCC AATGCCCTTC 
ATGGCAGAGA CAGCTAAGCT AGGCAGACCC AGATCTATAG ATCCTGGATT ACACAACGAG 
GCTCTACTTC CTGGAGACTG GGGACATATC CTCCGCCCTG AGGACCAAGG TGAGCCTGTG 
ACTATCAGCC ACACAAGCAA CCCTAGACTG CAGGAGGTGC AGACCCCAGA ATCCCTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col20a1 ( XM_017319287.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174776.1 Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31239
Clone ID Related Accession (Same CDS sequence)	XM_017319287.1
Accession Version	XM_017319287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTCC AAGGCTCCTA CCAACACTTC TGCCTCTGGA TGTTCTTGGG CACCACGTTG 
GCACTGGGGC AGGGACAAGT AAGTAGCCGC CTGAGGCTGG CAGTACTGCC TGAGGACCAG 
CTGCAGATGA AGTGGAGGGA AGCAGAGGGA AGTGGCCTTG GCTTCTTGGT GCAGGTGACA 
CCTATGGCAG GGGACTTGGA GCAGGAGCTG ATACTGACCA CCAAGACCCC CAAGGCCACT 
GTGGGTGGCC TGAACCCTTC CAAGAGCTAC ACCTTGCAGA TCTTTGAGCT CACTGACTCG 
GGGCCTATCC TGCTGGCACG AAGGGAGTTT GTGATTGAGG ATCTGAAGAG CCAATCTTTG 
GGCAGAGGCA GCCGGAGGCT GGCTGGAGCC ACCCTGGAGC CTACCTCCTT GCCTCTGAGG 
GGCCCAGACT CTGAGAAAAC ATCTGAGCCC AGCATTGCCT TTACCTTAAG CAGGGACCTG 
CCCATTCTTG ATCACCCCCA GTTCCAATGT ACACCCCCAA CACCTGCTGA TATAATCTTC 
CTGGTGGACG GGTCCTGGAG CATCGGCCAC AACCACTTCC AGCAGGTGAA AGACTTCCTG 
GCCAGCATCA TCACACAATT TGCAATAGGA CCAGATAAAG TCCAAGTAGG TCTGACACAG 
TACAGTGGAG ACCCCCAGAC CGAGTGGGAC CTGAACTCCT TCCAAACCAA GGAGCAAGTG 
CTAGCAGCTG TACACCACCT CCGCTACAAG GGAGGAAACA CATTCACAGG CCTTGCTCTA 
ACCCATGTGC TGGAGCAGAA CCTGAAGCCA GCAGCCGGTG TCCGTCCAGA GGCAGCCAAA 
GTGTTGATTC TGGTGACAGA TGGCAAGTCC CAGGATGATG TGCGCACAGC TGCCAGGATC 
CTTAAGGATC AGGACATTGA TGTCTTTGTT GTGGGTGTGA AGAATGTCGA TGAGGCTGAG 
CTGAAGCTCC TGGCGTCCCA GCCACTGGAC ATCACTGTCC ACAACGTGCT GGACTTCCCT 
CAGCTGGACA CCCTAGCTCC TCTGCTCAGC CGACTCATTT GCCAAAAAAT TCAGGGCAGA 
GGCCCAGTCA AACCAGCAGC AGGTACTCGG GTCCTGGACC CCCTCCCCAC GCCCACCAGA 
CTGATCCTGA CCCACGCCAC CTCATCCAGT ATCCACCTGT CTTGGACTCC AGCCTTATAC 
CCACCCCTCA AGTATCTAAT TGTATGGCAG CCTTCTAGGG GTGGTGCCCC CAAGGAGGTG 
GTGGTGGAGG GGCCTGTCTC ATCCATGGAG CTTGGAAACC TGACCTCCAG CACAGAATAC 
TTGGTCTCTG TGCTTCCTGT CTATGAGAGT GGGGTTGGCA AGAGCCTGCA AGGCCGGGCA 
ACCACAGCAC CCCTGCCTCC ACCTGGGCCA CTGACCCTAG CTGCAGTGAC ACCCAGAACT 
CTCCACGTCA CCTGGCCGCC CTCTGCTGGG GTCACTCAGT ACTTGGTGCA GTACTTGCTG 
GCTACCTCCA CTGGGGAGGA GCAAAAGAGA GAGGTACATG TGGGACAGCC TGAGGTACTG 
CTGGATGGTC TGGAGCCTGG CCAGGACTAT GATGTCTCAG TGCAGAGCCT TCGGGGACCA 
GAGGCCAGCG AGGTCCAGAG CATCCGTGCC AGGACCTCTG CCCTGGGGCC CCCAAGACAC 
CTGACTTTCT CAGATGTTCG CTACAACTCA ACCTGTGTGT CCTGGGAAGC CCAGAGGCCT 
GTGCGCCTGG TCAAGGTCAG CTACATCTCT AGTGATGGCA GCCACTCTGG GCAGACTCAG 
GTTCCTGGGA ACCTTACTTC AGCTACTCTG GGCCCACTTT CTTCCTCTAC CATGTACACC 
GTCAGAGTCA CCTGTTTTTA CCTTGGGGGT GGTTCCTCCG TGCTGACTGG CCATGTAACC 
ACACAGAAAG CTCCTAGCCC TGGCCAGCTG TCTGTGATGG AGCTCCCGGG AGATGCAGTA 
AAGCTGTCAT GGCTGGCCAC TGCCTTGTCT GGTGTACTTG TTTATCAGAT CAAGTGGATG 
CCCTTGGGAG AAGGGAAGGC CCGTGAGATC TCTGTCCCAG GGACCCTTGG CACAGCCACC 
TTGCCTGGCC TGATGAAACA TGTGGAATAT GAGATCACCA TCCTTGCCTA CTACAGGGAC 
GGCACTCGCA GCGACCCCGT GTCCCTCCGC TACACCCCCT CTGCAGCAAG CAGAAGCCCC 
CCATCCAGCC TGGCCTTGTC CTCGGAGACC CCCAACAGCC TGCAGGTCAA CTGGACACCA 
CCAAGTGGCC ATGTCCTCCA TTACCGGCTC AACTACACAC TGGCCTCAGG CTCTGGACCA 
GAGAAATCGA TCTCTGTACC AGGCACCAGG AGCCATGCCG TGCTTCGTGA TCTGATGTCT 
GCCACGAAGT ACAGGGTCCT GGTCTCAGCT GTCTACAGAG CAGGGGAGAG CATGGCAGTG 
TCTGCCACAT ACCGCACAGC CTGCCCAGCC CTGCACCCGG ACAGTTCCCT CTCAGGGTTT 
GACCTGATGG TGGCCTTTGG CCTCGTGGCA AAGGAGTATG CCTCTATTCG AGGAGTTGCC 
ATGGAGCCCT CAGCCTTGGG TGTGGTCCCA ACCTTCACTC TCTTCAAGGA TGCTCAACTG 
ATGCGTCGGG TCAGTGACAT CTACCCAGCA ACCCTGCCAC CAGAACACAC CATTGTCTTC 
CTTGTGCGTC TACTCCCTGA GACACCCCGG GAAGCCTTTG CACTGTGGCA GATGATGGCT 
GAAGATTTCC AGCCCATCCT CGGGGTTCTG CTGGATGCTG GCAGAAAGTC TCTGACTTAC 
TTCAACCATG ATTCCCGGGC AGCCTTGCAG GAGGTCACCT TTGACCTGCA GGATGCAAAG 
AAGATCTTCT TTGGAAGCTT CCACAAGGTA CATATAGCTG TAGGCCACTC TAAGGTCAGA 
CTCTATGTGG ACTGTCGGAA GGTGGCCGAG CGTCCCATTG GGGATGCTGG CAGCCCACCC 
ACAGGTGGTT TCATCACACT AGGGAGGCTA GCCAAGGCCA GAGGGCCACG GAGCAGCTCA 
GCCACATTCC AGCTCCAGAT GTTGCAAATC GTGTGCAGTG ATACCTGGGC AGACAAGGAC 
AGGTGTTGTG AGATTCCTGC ACTGAGGGAT GGAGAGACGT GCCCGGCCTT CCCTTCAGCC 
TGTGCCTACT CATCTGAGAC CCCTGGGCCA CCAGGGCCCC AAGGCCCTCC GGGTCTCCCT 
GGAAGGAACG GCCCTCCAGG ACAGCAGGGG CACCCAGGGC CCAAGGGGGA GCCGGGCCCA 
CCTGGACAAA CAGGACCTGA AGGCCCTGGA GGTCAGCAGG GGTCACCAGG GACCCAGGGC 
CGCGCAGTCC AGGGACCCAT GGGGCCACCA GGAGCCAAAG GAGAGAAGGG GGATCAGGGA 
CTTTCAGGCT TGCAGGGCCT CTCTGGCCAG CAAGGCATCC CTGGGAAAAC TGGCCTACAG 
GGACCAAAGG GCATGAGAGG ACTGGAGGGA CCTGCTGGCC TGCCAGGACC ACCTGGCCCC 
AGGGGGTTCC AGGGCTTGGC AGGGGCCAGG GGCACCAATG GGGAGCGAGG AGCCCCAGGA 
GCTGTGGGTC CCACAGGTCT ACCAGGGTCC AAAGGTGAAC GAGGAGAGAA GGGAGAGCCA 
CAGTCCCTTG CCACCATCTT CCAGCTGGTG AGCCAGGCCT GCGAGTCAGC CATACGGACA 
CATGTGCTGA AGCTGAATTC CTTCCTCCAT GAGAATGCCC GTCCCCCAAT GCCCTTCATG 
GCAGAGACAG CTAAGCTAGG CAGACCCAGA TCTATAGATC CTGGATTACA CAACGAGGCT 
CTACTTCCTG GAGACTGGGG ACATATCCTC CGCCCTGAGG ACCAAGGTGA GCCTGTGACT 
ATCAGCCACA CAAGCAACCC TAGACTGCAG GAGGTGCAGA CCCCAGAATC CCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174776.1
CDS	3213..7172
Translation	MSLQGSYQHFCLWMFLGTTLALGQGQVSSRLRLAVLPEDQLQMKWREAEGSGLGFLVQVTPMAGDLEQELILTTKTPKATVGGLNPSKSYTLQIFELTDSGPILLARREFVIEDLKSQSLGRGSRRLAGATLEPTSLPLRGPDSEKTSEPSIAFTLSRDLPILDHPQFQCTPPTPADIIFLVDGSWSIGHNHFQQVKDFLASIITQFAIGPDKVQVGLTQYSGDPQTEWDLNSFQTKEQVLAAVHHLRYKGGNTFTGLALTHVLEQNLKPAAGVRPEAAKVLILVTDGKSQDDVRTAARILKDQDIDVFVVGVKNVDEAELKLLASQPLDITVHNVLDFPQLDTLAPLLSRLICQKIQGRGPVKPAAGTRVLDPLPTPTRLILTHATSSSIHLSWTPALYPPLKYLIVWQPSRGGAPKEVVVEGPVSSMELGNLTSSTEYLVSVLPVYESGVGKSLQGRATTAPLPPPGPLTLAAVTPRTLHVTWPPSAGVTQYLVQYLLATSTGEEQKREVHVGQPEVLLDGLEPGQDYDVSVQSLRGPEASEVQSIRARTSALGPPRHLTFSDVRYNSTCVSWEAQRPVRLVKVSYISSDGSHSGQTQVPGNLTSATLGPLSSSTMYTVRVTCFYLGGGSSVLTGHVTTQKAPSPGQLSVMELPGDAVKLSWLATALSGVLVYQIKWMPLGEGKAREISVPGTLGTATLPGLMKHVEYEITILAYYRDGTRSDPVSLRYTPSAASRSPPSSLALSSETPNSLQVNWTPPSGHVLHYRLNYTLASGSGPEKSISVPGTRSHAVLRDLMSATKYRVLVSAVYRAGESMAVSATYRTACPALHPDSSLSGFDLMVAFGLVAKEYASIRGVAMEPSALGVVPTFTLFKDAQLMRRVSDIYPATLPPEHTIVFLVRLLPETPREAFALWQMMAEDFQPILGVLLDAGRKSLTYFNHDSRAALQEVTFDLQDAKKIFFGSFHKVHIAVGHSKVRLYVDCRKVAERPIGDAGSPPTGGFITLGRLAKARGPRSSSATFQLQMLQIVCSDTWADKDRCCEIPALRDGETCPAFPSACAYSSETPGPPGPQGPPGLPGRNGPPGQQGHPGPKGEPGPPGQTGPEGPGGQQGSPGTQGRAVQGPMGPPGAKGEKGDQGLSGLQGLSGQQGIPGKTGLQGPKGMRGLEGPAGLPGPPGPRGFQGLAGARGTNGERGAPGAVGPTGLPGSKGERGEKGEPQSLATIFQLVSQACESAIRTHVLKLNSFLHENARPPMPFMAETAKLGRPRSIDPGLHNEALLPGDWGHILRPEDQGEPVTISHTSNPRLQEVQTPESLE

Target ORF information:

RefSeq Version	XM_017319287.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XX, alpha 1 (Col20a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319287.1

ORF Insert Sequence:

ATGAGCCTCC AAGGCTCCTA CCAACACTTC TGCCTCTGGA TGTTCTTGGG CACCACGTTG 
GCACTGGGGC AGGGACAAGT AAGTAGCCGC CTGAGGCTGG CAGTACTGCC TGAGGACCAG 
CTGCAGATGA AGTGGAGGGA AGCAGAGGGA AGTGGCCTTG GCTTCTTGGT GCAGGTGACA 
CCTATGGCAG GGGACTTGGA GCAGGAGCTG ATACTGACCA CCAAGACCCC CAAGGCCACT 
GTGGGTGGCC TGAACCCTTC CAAGAGCTAC ACCTTGCAGA TCTTTGAGCT CACTGACTCG 
GGGCCTATCC TGCTGGCACG AAGGGAGTTT GTGATTGAGG ATCTGAAGAG CCAATCTTTG 
GGCAGAGGCA GCCGGAGGCT GGCTGGAGCC ACCCTGGAGC CTACCTCCTT GCCTCTGAGG 
GGCCCAGACT CTGAGAAAAC ATCTGAGCCC AGCATTGCCT TTACCTTAAG CAGGGACCTG 
CCCATTCTTG ATCACCCCCA GTTCCAATGT ACACCCCCAA CACCTGCTGA TATAATCTTC 
CTGGTGGACG GGTCCTGGAG CATCGGCCAC AACCACTTCC AGCAGGTGAA AGACTTCCTG 
GCCAGCATCA TCACACAATT TGCAATAGGA CCAGATAAAG TCCAAGTAGG TCTGACACAG 
TACAGTGGAG ACCCCCAGAC CGAGTGGGAC CTGAACTCCT TCCAAACCAA GGAGCAAGTG 
CTAGCAGCTG TACACCACCT CCGCTACAAG GGAGGAAACA CATTCACAGG CCTTGCTCTA 
ACCCATGTGC TGGAGCAGAA CCTGAAGCCA GCAGCCGGTG TCCGTCCAGA GGCAGCCAAA 
GTGTTGATTC TGGTGACAGA TGGCAAGTCC CAGGATGATG TGCGCACAGC TGCCAGGATC 
CTTAAGGATC AGGACATTGA TGTCTTTGTT GTGGGTGTGA AGAATGTCGA TGAGGCTGAG 
CTGAAGCTCC TGGCGTCCCA GCCACTGGAC ATCACTGTCC ACAACGTGCT GGACTTCCCT 
CAGCTGGACA CCCTAGCTCC TCTGCTCAGC CGACTCATTT GCCAAAAAAT TCAGGGCAGA 
GGCCCAGTCA AACCAGCAGC AGGTACTCGG GTCCTGGACC CCCTCCCCAC GCCCACCAGA 
CTGATCCTGA CCCACGCCAC CTCATCCAGT ATCCACCTGT CTTGGACTCC AGCCTTATAC 
CCACCCCTCA AGTATCTAAT TGTATGGCAG CCTTCTAGGG GTGGTGCCCC CAAGGAGGTG 
GTGGTGGAGG GGCCTGTCTC ATCCATGGAG CTTGGAAACC TGACCTCCAG CACAGAATAC 
TTGGTCTCTG TGCTTCCTGT CTATGAGAGT GGGGTTGGCA AGAGCCTGCA AGGCCGGGCA 
ACCACAGCAC CCCTGCCTCC ACCTGGGCCA CTGACCCTAG CTGCAGTGAC ACCCAGAACT 
CTCCACGTCA CCTGGCCGCC CTCTGCTGGG GTCACTCAGT ACTTGGTGCA GTACTTGCTG 
GCTACCTCCA CTGGGGAGGA GCAAAAGAGA GAGGTACATG TGGGACAGCC TGAGGTACTG 
CTGGATGGTC TGGAGCCTGG CCAGGACTAT GATGTCTCAG TGCAGAGCCT TCGGGGACCA 
GAGGCCAGCG AGGTCCAGAG CATCCGTGCC AGGACCTCTG CCCTGGGGCC CCCAAGACAC 
CTGACTTTCT CAGATGTTCG CTACAACTCA ACCTGTGTGT CCTGGGAAGC CCAGAGGCCT 
GTGCGCCTGG TCAAGGTCAG CTACATCTCT AGTGATGGCA GCCACTCTGG GCAGACTCAG 
GTTCCTGGGA ACCTTACTTC AGCTACTCTG GGCCCACTTT CTTCCTCTAC CATGTACACC 
GTCAGAGTCA CCTGTTTTTA CCTTGGGGGT GGTTCCTCCG TGCTGACTGG CCATGTAACC 
ACACAGAAAG CTCCTAGCCC TGGCCAGCTG TCTGTGATGG AGCTCCCGGG AGATGCAGTA 
AAGCTGTCAT GGCTGGCCAC TGCCTTGTCT GGTGTACTTG TTTATCAGAT CAAGTGGATG 
CCCTTGGGAG AAGGGAAGGC CCGTGAGATC TCTGTCCCAG GGACCCTTGG CACAGCCACC 
TTGCCTGGCC TGATGAAACA TGTGGAATAT GAGATCACCA TCCTTGCCTA CTACAGGGAC 
GGCACTCGCA GCGACCCCGT GTCCCTCCGC TACACCCCCT CTGCAGCAAG CAGAAGCCCC 
CCATCCAGCC TGGCCTTGTC CTCGGAGACC CCCAACAGCC TGCAGGTCAA CTGGACACCA 
CCAAGTGGCC ATGTCCTCCA TTACCGGCTC AACTACACAC TGGCCTCAGG CTCTGGACCA 
GAGAAATCGA TCTCTGTACC AGGCACCAGG AGCCATGCCG TGCTTCGTGA TCTGATGTCT 
GCCACGAAGT ACAGGGTCCT GGTCTCAGCT GTCTACAGAG CAGGGGAGAG CATGGCAGTG 
TCTGCCACAT ACCGCACAGC CTGCCCAGCC CTGCACCCGG ACAGTTCCCT CTCAGGGTTT 
GACCTGATGG TGGCCTTTGG CCTCGTGGCA AAGGAGTATG CCTCTATTCG AGGAGTTGCC 
ATGGAGCCCT CAGCCTTGGG TGTGGTCCCA ACCTTCACTC TCTTCAAGGA TGCTCAACTG 
ATGCGTCGGG TCAGTGACAT CTACCCAGCA ACCCTGCCAC CAGAACACAC CATTGTCTTC 
CTTGTGCGTC TACTCCCTGA GACACCCCGG GAAGCCTTTG CACTGTGGCA GATGATGGCT 
GAAGATTTCC AGCCCATCCT CGGGGTTCTG CTGGATGCTG GCAGAAAGTC TCTGACTTAC 
TTCAACCATG ATTCCCGGGC AGCCTTGCAG GAGGTCACCT TTGACCTGCA GGATGCAAAG 
AAGATCTTCT TTGGAAGCTT CCACAAGGTA CATATAGCTG TAGGCCACTC TAAGGTCAGA 
CTCTATGTGG ACTGTCGGAA GGTGGCCGAG CGTCCCATTG GGGATGCTGG CAGCCCACCC 
ACAGGTGGTT TCATCACACT AGGGAGGCTA GCCAAGGCCA GAGGGCCACG GAGCAGCTCA 
GCCACATTCC AGCTCCAGAT GTTGCAAATC GTGTGCAGTG ATACCTGGGC AGACAAGGAC 
AGGTGTTGTG AGATTCCTGC ACTGAGGGAT GGAGAGACGT GCCCGGCCTT CCCTTCAGCC 
TGTGCCTACT CATCTGAGAC CCCTGGGCCA CCAGGGCCCC AAGGCCCTCC GGGTCTCCCT 
GGAAGGAACG GCCCTCCAGG ACAGCAGGGG CACCCAGGGC CCAAGGGGGA GCCGGGCCCA 
CCTGGACAAA CAGGACCTGA AGGCCCTGGA GGTCAGCAGG GGTCACCAGG GACCCAGGGC 
CGCGCAGTCC AGGGACCCAT GGGGCCACCA GGAGCCAAAG GAGAGAAGGG GGATCAGGGA 
CTTTCAGGCT TGCAGGGCCT CTCTGGCCAG CAAGGCATCC CTGGGAAAAC TGGCCTACAG 
GGACCAAAGG GCATGAGAGG ACTGGAGGGA CCTGCTGGCC TGCCAGGACC ACCTGGCCCC 
AGGGGGTTCC AGGGCTTGGC AGGGGCCAGG GGCACCAATG GGGAGCGAGG AGCCCCAGGA 
GCTGTGGGTC CCACAGGTCT ACCAGGGTCC AAAGGTGAAC GAGGAGAGAA GGGAGAGCCA 
CAGTCCCTTG CCACCATCTT CCAGCTGGTG AGCCAGGCCT GCGAGTCAGC CATACGGACA 
CATGTGCTGA AGCTGAATTC CTTCCTCCAT GAGAATGCCC GTCCCCCAAT GCCCTTCATG 
GCAGAGACAG CTAAGCTAGG CAGACCCAGA TCTATAGATC CTGGATTACA CAACGAGGCT 
CTACTTCCTG GAGACTGGGG ACATATCCTC CGCCCTGAGG ACCAAGGTGA GCCTGTGACT 
ATCAGCCACA CAAGCAACCC TAGACTGCAG GAGGTGCAGA CCCCAGAATC CCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM

The Vertebrate Genome Annotation browser 10 years on.
Nucleic acids research42(Database issue)D771-9(2014 Jan)
Harrow JL,Steward CA,Frankish A,Gilbert JG,Gonzalez JM,Loveland JE,Mudge J,Sheppard D,Thomas M,Trevanion S,Wilming LG

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Col20a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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