Vps50 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps50
Entrez Gene ID	73288
Full Name	VPS50 EARP/GARPII complex subunit
Synonyms	1700034M03Rik,8430415E05Rik,BLM,C87205,Ccdc132,mKIAA1861
General protein information	Preferred Names VPS50 EARP/GARPII complex subunit Names syndetin EARP/GARPII complex subunit VPS50 bcl2-like protein blm coiled-coil domain containing 132 coiled-coil domain-containing protein 132
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001167750.1	NP_001161222.1	syndetin isoform 2
NM_001167751.1	NP_001161223.1	syndetin isoform 3
NM_024260.5	NP_077222.4	syndetin isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCDC132	NP_060137.2
Pan troglodytes (chimpanzee)	CCDC132	XP_519203.3
Bos taurus (cattle)	CCDC132	NP_001098883.1
Canis lupus familiaris (dog)	CCDC132	XP_532460.4
Mus musculus (house mouse)	Ccdc132	NP_077222.4
Gallus gallus (chicken)	CCDC132	NP_001012872.1
Arabidopsis thaliana (thale cress)	AT2G27900	NP_001031433.1
Oryza sativa (rice)	Os10g0546300	NP_001065215.1
Rattus norvegicus (Norway rat)	Ccdc132	NP_001166982.1
Caenorhabditis elegans (roundworm)	C44B9.1	NP_499394.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000343	XP_310775.5
Macaca mulatta (Rhesus monkey)	CCDC132	XP_001090270.2
Drosophila melanogaster (fruit fly)	CG4996	NP_611258.2
Danio rerio (zebrafish)	si:ch211-287b5.1	XP_001923725.4
Xenopus tropicalis (tropical clawed frog)	ccdc132	NP_001107716.1

Gene Ontology
Bibliography

Related articles in PubMed

The Conserved VPS-50 Protein Functions in Dense-Core Vesicle Maturation and Acidification and Controls Animal Behavior.
Paquin N, Murata Y, Froehlich A, Omura DT, Ailion M, Pender CL, Constantine-Paton M, Horvitz HR
Current biology : CB26(7)862-71(2016 Apr)

EARP is a multisubunit tethering complex involved in endocytic recycling.
Schindler C, Chen Y, Pu J, Guo X, Bonifacino JS
Nature cell biology17(5)639-50(2015 May)

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Tkatchenko TV, Moreno-Rodriguez RA, Conway SJ, Molkentin JD, Markwald RR, Tkatchenko AV
Physiological genomics39(3)160-8(2009 Nov)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11115	NM_001167751.1 Latest version!	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu05693	NM_024260.5 Latest version!	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$566.30 $809.00
OMu11113	NM_001167750.1 Latest version!	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$545.30 $779.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps50 ( NM_001167751.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161223.1 Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu11115
Clone ID Related Accession (Same CDS sequence)	NM_001167751.1
Accession Version	NM_001167751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559750400000
Organism	Mus musculus(house mouse)
Product	syndetin isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY100563.1, BC043092.1, AK042031.1 and AC066688.4. Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK042031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGGTAAG AACACGGGTT GGTGTGCTGA TCTTGTCTGG GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161223.1
CDS	131..2947
Translation	MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSADPFDIVKYELEKLPPVLNLQELEEYRDKLKQQQSAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHDNEETAAAAEGSNVMSTEEATFDRGYVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSSKPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLKTTLNRIQESLIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQHNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHRVRTRVGVLILSGV

Target ORF information:

RefSeq Version	NM_001167751.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167751.1

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGGTAAG AACACGGGTT GGTGTGCTGA TCTTGTCTGG GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps50 ( NM_024260.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077222.4 Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05693
Clone ID Related Accession (Same CDS sequence)	NM_024260.5
Accession Version	NM_024260.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Mus musculus(house mouse)
Product	syndetin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY100563.1, AK129458.1 and AI551221.1. On Nov 17, 2009 this sequence version replaced NM_024260.4. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK075898.1, SRR1660821.43384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGGAATA CTCAACGAAG CAGTTGACCA ACCTGGTGAA TGTTTGCCTA 
GGATCTCATA TCAATAAGAA AGCAAGACAG AAGCTCCTAG CAGCTATAGA TGAGATAGAC 
AGGCCTAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077222.4
CDS	131..3025
Translation	MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSADPFDIVKYELEKLPPVLNLQELEEYRDKLKQQQSAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHDNEETAAAAEGSNVMSTEEATFDRGYVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSSKPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLKTTLNRIQESLIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQHNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDEIDRPKR

Target ORF information:

RefSeq Version	NM_024260.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024260.5

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGGAATA CTCAACGAAG CAGTTGACCA ACCTGGTGAA TGTTTGCCTA 
GGATCTCATA TCAATAAGAA AGCAAGACAG AAGCTCCTAG CAGCTATAGA TGAGATAGAC 
AGGCCTAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps50 ( NM_001167750.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161222.1 Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 2, mRNA.

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CloneID	OMu11113
Clone ID Related Accession (Same CDS sequence)	NM_001167750.1
Accession Version	NM_001167750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Mus musculus(house mouse)
Product	syndetin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY100563.1, BC043092.1, AC066688.4 and AI551221.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC043092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGATTTT CTCTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161222.1
CDS	131..2920
Translation	MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSADPFDIVKYELEKLPPVLNLQELEEYRDKLKQQQSAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHDNEETAAAAEGSNVMSTEEATFDRGYVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSSKPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLKTTLNRIQESLIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQHNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHRIFSV

Target ORF information:

RefSeq Version	NM_001167750.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167750.1

ORF Insert Sequence:

ATGCAAAAAA TCAAATCTCT CATGACCCGG CAGGGTCTGA AGAGCCCTCC GGAGAGCCTC 
AATGATCTTG GTGCCTTTGA GAGCCTTCGG GTCCCTGGAA AGGAGGAATT CAGGGAGCTT 
CGAGAACAGC CAAGCGACCC TCAAGCTGAG CAAGAGTTGA TTAATAGCAT TGAACAAGTC 
TATTTTTCTG CGGATCCATT TGATATTGTT AAATATGAGC TGGAGAAGCT TCCTCCTGTT 
CTCAATCTGC AAGAGTTAGA GGAGTACAGA GACAAATTAA AGCAACAACA ATCTGCAGTC 
TCTAAAAAAG TGGCAGATTT GATTCTTGAA AAGCAGCCTG CTTATGTAAA GGAACTTGAA 
AGAGTCACAT CACTGCAGAC AGGTCTTCAG TTGGCTGCTG TCATCTGCAC AAATGGGAGG 
AGGCACTTGA ATATTGCAAA GGAAGGTTTT ACTCAGGCTA GCTTAGGCCT TCTTGCAAAT 
CAAAGGAAGC GTCAGTTGCT AATTGGACTT CTGAAGTCGC TGAGAACCAT AAAAACATTG 
CAAAGAACTG ACATACGCTT AAGTGAAATG CTGGAGGAAG AGGACTATCC AGGAGCTATT 
CAGCTGTGCC TAGAATGTCA GAAGGCTGCC AGCACTTTTA AACACTACAG CTGTATAAGT 
GAGCTGAATT CAAAGCTGCA AGATACTTTG GAGCAAATTG AGGAACAGTT GGATGTAGCT 
CTTTCCAAAA TCTGCAAGAA TTTTGACATT AACCATTATA CCAAGGTTCA GCAAGCTTAC 
CGGCTTCTTG GAAAAACACA GACTGCGATG GATCAGCTTC ATATGCACTT CACTCAAGCC 
ATTCACAACA CCGTATTTCA GGTTGTGCTT GGCTATGTGG AGCTGTGTGC AGGAAACACG 
GATACAAAAT TCCAGAAGCT TCAGTATAAA GATCTCTGTA CGCATGTGAC ACCAGACAGC 
TATATTCCAT GCCTGGCAGA CCTGTGCAAA GCACTGTGGG AAGTCATGCT CAGCTACTAC 
AGAACCATGG AGTGGCACGA GAAACATGAC AATGAGGAAA CAGCTGCAGC TGCTGAAGGA 
AGCAATGTGA TGAGTACTGA GGAGGCCACT TTTGACCGTG GCTATGTAAA GAAGAAGTTA 
GAGCACGGCC TTACCCGGAT ATGGCAGGAT GTTCAACTAA AAGTAAAAAC CTACTTGCTT 
GGAACTGATT TATCTATATT CAAATATGAT GATTTCATCT TTGTTCTGGA TATTGTCAGC 
AGGTTGATGC AAGTTGGAGA AGAATTTTGT GGTAGCAAGT CTGAAGTTTT GCAGGAGTCC 
ATTAGAAAAC AAAGTGTCAA TTACTTCAAG AACCACCATA GGATACGACT TGATGAACTG 
AGAATGTTCT TAGAGAATGA GACATGGGAG CTTTGCCCTG TTAAGTCAAA TTTTAGCATC 
TTGCAGCTTC ATGAGTTTAA ATTCCTGGAA CAGTCTCGCT CCCCATCAGT TTCACCTAGT 
AAGCAGCCAT CAGCAACTTC TTCAAAGCCT GTGACTTTGT TTGAGCAGTA CTGCAGTGGT 
GGGAATCCAT TTGAAATACA AGCCGACCAC AAAGATGAAG AAACTGAAGA TGTCTTAGCC 
TCTAATGGGT ATGAGTCTGA TGAGCAAGAG AAGAGCGCCT ATCAGGACTA TGACAGTGAC 
AGCGACGTCC CTGAGGAGCT GAAGCGGGAT TACGTGGACG AGCAGACCGG CGACGTGCCC 
GTGAAGAGTG TTTCCCGAGA GACCCTGAAG AGCAGGAAGA AGTCGGATTA CAGTCTAAAC 
AAAGTGAATG CACCCATCTT AACAAACACA ACACTGAATG TAATAAGGCT TGTTGGAAAG 
TACATGCAGA TGATGAACAT CCTTAAGCCA ATCGCCTTCG ATGTGATCCA TTTCATGTCT 
CAGCTCTTTG ACTATTACTT GTATGCCATA TATACCTTTT TTGGTCGGAA TGATTCATTG 
GAATCAACAG GACTTGGCCT TAGTAGTAGC AGACTAAAAA CAACTCTAAA CAGAATTCAA 
GAAAGCCTTA TTGACCTGGA GGGCTCAGCT GATCCAACTG CCACTCTCAC AGCAGCCGAG 
GAAAGAAAGG AGAAGGTACC CAGCCCACAC CTCAATCAGC TAGTGATTTT GACATCTGGG 
GATACATTGT ATGGGTTGGC AGAGAGAGTG GTAGCCACAG AATCCTTAGT GTTCTTGGCT 
GAGCAGTTTG AGTTCCTTCA GCCACATTTG GATGCTGTGA TGCCTGCAGT CAAAAAGCCC 
TTCCTGCAGC AGTTTTACTC CCAGACAGTC TCAACTGCCA GTGAACTGCG CAAGCCCATT 
TACTGGATTG TGGCTGGTAA AGCTATTGAT TATGAACAGA TGCTTCTCCT AATGATGAAT 
GTGAAGTGGG ATGTCAAAGA GATCATGTCA CAGCACAACA TCTATGTAGA TGCATTGCTG 
AAGGAATTTG AGCAGTTTAA CAAGCGGTTA AATGAAGTTT CTAAGAGAGT TCGGATACCT 
CTGCCTGTAT CAAATATACT TTGGGAGCAT TGTATACGAT TAGCTAACAG AACTATTGTG 
GAAGGATACG CCAATGTCAA GAAATGCAGT AATGAGGGGC GTGCCTTGAT GCAATTGGAT 
TTTCAACAGT TTTTAATGAA ACTCGAAAAA CTAACTGACA TTAGACCTAT TCCTGATAAA 
GAATTTGTGG AAACGTATAT CAAAGCCTAC TACCTAACTG AGAATGACAT GGAGCGTTGG 
ATCAAAGAGC ACAGGATTTT CTCTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Conserved VPS-50 Protein Functions in Dense-Core Vesicle Maturation and Acidification and Controls Animal Behavior.
Current biology : CB26(7)862-71(2016 Apr)
Paquin N,Murata Y,Froehlich A,Omura DT,Ailion M,Pender CL,Constantine-Paton M,Horvitz HR

EARP is a multisubunit tethering complex involved in endocytic recycling.
Nature cell biology17(5)639-50(2015 May)
Schindler C,Chen Y,Pu J,Guo X,Bonifacino JS

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Physiological genomics39(3)160-8(2009 Nov)
Tkatchenko TV,Moreno-Rodriguez RA,Conway SJ,Molkentin JD,Markwald RR,Tkatchenko AV

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Vps50 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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