Vps50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps50
Entrez Gene ID	37021
Full Name	Vacuolar protein sorting 54L
Synonyms	CG4996,Dmel\CG4996
General protein information	Preferred Names Vacuolar protein sorting 54L Names CG4996 gene product from transcript CG4996-RA CG4996-PA Vps54L-PA vacuolar protein sorting 54L
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E5-54E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_137414.3	NP_611258.2	vacuolar protein sorting 50

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCDC132	NP_060137.2
Pan troglodytes (chimpanzee)	CCDC132	XP_519203.3
Bos taurus (cattle)	CCDC132	NP_001098883.1
Canis lupus familiaris (dog)	CCDC132	XP_532460.4
Mus musculus (house mouse)	Ccdc132	NP_077222.4
Gallus gallus (chicken)	CCDC132	NP_001012872.1
Arabidopsis thaliana (thale cress)	AT2G27900	NP_001031433.1
Oryza sativa (rice)	Os10g0546300	NP_001065215.1
Rattus norvegicus (Norway rat)	Ccdc132	NP_001166982.1
Caenorhabditis elegans (roundworm)	C44B9.1	NP_499394.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000343	XP_310775.5
Macaca mulatta (Rhesus monkey)	CCDC132	XP_001090270.2
Drosophila melanogaster (fruit fly)	CG4996	NP_611258.2
Danio rerio (zebrafish)	si:ch211-287b5.1	XP_001923725.4
Xenopus tropicalis (tropical clawed frog)	ccdc132	NP_001107716.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10382	NM_137414.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 50 (Vps50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps50 ( NM_137414.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611258.2 Drosophila melanogaster vacuolar protein sorting 50 (Vps50), mRNA.

Price & Availability↑

CloneID	OFa10382
Clone ID Related Accession (Same CDS sequence)	NM_137414.3
Accession Version	NM_137414.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 50
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137414.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAATT CCAAGGCCAA AATGGACGAA TTCAAGAGCA AATTCTTGGA CATATTGCAC 
AAACAGACGA ACCGTCAAAT GAAAATTCCT GCCATGGGTT TTTCGGATTA TTTCATTCAA 
ACGGTGACCA CAGACGCCTC CGCCAGTCAA GAAACTGATG CCACAGACGC GGCAGCCACT 
TCTTCCAGCG AGTCAAATGC ACAAAAGTCG GACCAGGAGA TCCTGGAGAG CATCGAACAG 
TGCTACTACA ACCCCGACAG CAATCCACAG CTGTACGAAC TCAAGAAAGT TCTCAACGAT 
GGCATAGACA ACGAGCTCAT CGAGGTCACC ATTGCACAAT TGCGCACTCA GCAGAAGGTT 
CTTACCAAAC AGGTGCTCCA GAACATCCTG GAGCAGCGGA ATGCCTGCAG CAGTGAGTTT 
GCCGCTATCA ATGACACGCA GAAGAAGTTG GAGGAATCGC TGTGGACATG CCAAAAGGCG 
AGATCCTATT TAAACTTTGC TCGCACGAAC CTAACAACCA CTAGCCTGGA GATCTTGGCC 
AGCTACCGCA AACGAGAGGT GCTCAAGGAG GTGTTGGACA CCCTGGTGGC CATCAAGAGA 
CTGCGCACTA CTGACGTGGA GGTACAGAAG CTGCTAGCGG ATCACAACTA CAGCGCCGCC 
ATAGCTCTGC TCCTGCAGTG CAAGAGCTCC GCCGAGGCCT ACATGCAGTT CAACTGTGTA 
CAATCGCTCT CTAAAAAGCT GCAGGAAACC ATGCTGCTTA TGGAATTTCA GCTGGATACT 
GTGCTTAATG AAATGGTGCT AGGTTTTGAT GGGCGAAAAT ACGCCAAACT GCAGGAGGCC 
TACAAATTGG CAAACAAGTC ACTGATGGCC ATGGACCAGC TGCACATCAA CTATATTTCG 
GCCATTCACT CTTCTGTGAA TGCAGTGCTT CGCGGTTACA GCGATCCTGG CCAGGACGAT 
CAGGCCAAGC CGCTCTATGA GCAGCTGTGC GAACAGCTTA GCGTGGATAA GCTGCTGCCC 
TGTCTGATTA GTTTGTGCAA AACCTTCTGG ACCATTCTGG CCTCCTACTA TCAGGTTGTT 
ATGTGGCACA ATAACTACAA GTTGTATGGA CAGGAGGAGG AGGATTCACC AGATATTTAC 
ATTCAGCAGA AACTGAAGAA GGGACAGTCA CGCGTTTGGA ATGACATTCT GACCAAAGTG 
TGCTTGTTCC TGCAGTCGGC AAAGTTGACG GCTCTTAAAT ACGATCAGTT CATCCAAGTG 
CTGTCCGTGG TACAAAGACT TAAGAAGGTG GGTCTGGAGT TCTGCGGCGA GCAGTCGGAG 
AAGTTGATCG AGACGATGCA GCAGCAGAGC GAGGAGTTCT TCCGGCGTTA CCATATCTGC 
TGCATTGAGG AAATCTGTTT ATTCTTGGAT AACGAGTCCT GGACGCAAGT CGACTCATTT 
GCGCATATCC TGCAATTGCC TGAATTCCGA TCGGTTCGGC ACACTCTTCG GCGCCACAAG 
TCTCCGACAA CTGCTTTGCT GGCCTCATCC AACAACTCCC CGATCAGCAA TAATAACTGC 
GATGAGCTGG TTTCCGTGCA CTCCCAGGAT GGTGGCAGCT CCATTTACGG TTCCTACGGA 
TATTTTCTCA GATTCTCCGA AAAAAGTTCG CCATTTGATG GTGGCTTGGA TGCAGCAATG 
TTGGAGGAGG ACATCTTATC CGGCATTGTG GACGAGGCCT CCTGTTATTT CAGCGAGGAG 
AGCGATGACG AGCAGAAAAG CTTGCAAAGC AAGGAGTTCG ATGACGCCAG CAACCAGCTG 
CTGGTAAACA ACACTGCGCT AAATGTGATG CGCGTCATTG GAAGATATCT GCAGATGTGC 
AAGTTATTGC ACTGCATCTC TCCCAAGATT GTGGCTTGCA TCCTGGAGCT CATTGACTTT 
TATGTCTACG CAGTGCACGA AATCTTCGGA AAAGATGCGC CGGTAGCAAC GGATAATATG 
CACTCTCCTA GACTGGAGCA ACGTTTACGC TCGGTCGAGG TTAACGTTCT GCCACAAATC 
AAGCTATGGC CCCTTAATTT CTCCTCTCTG GCAAATAATG AGCTAGCTAA TCCGGATACC 
TTATACGGAC TGTCACAACG CATCGTGGCC GTGGAAGCGG GTCGCAGCAT GCTTCAGCAG 
TTCAACGTCC TTCAGCACTA TCTGAACCAC TTGCTGCCCC TTGCAGAGCG TCCAATCCTG 
GCCAGTTACC TGGACTATAG CGATTATATT GCGGACTTGG CTAAACTCGT TTACACATGC 
GTCACGTCGC GCGTCATCGA TTTGTCATCC ATTCTGGCTC AGATGGCCAA GGTCAAATGG 
GATGTTAATC ATGTGATACA CCAGCACAGC AACTACAGTG ATGTTCTAAA TAGGAACATA 
CAAAGCTTCG CCATGTGTCT GGAGGAAATT GCCAAGGAGG TTCCAGTGCC CAGCAAGCAA 
GTGTGGAATT CAATGGCCCA TGTGGCCACG CATCTGCTGG TGGAGGGGTT CTCCAATGTG 
AAGAAATGCT CTGCCGGAGG ACGAGCGCTT ATGCAACTGG ATTTTGCGAA CTTTATGTCG 
TTCCTAGAGC TCATATCGAA CCAAAAGTAC CCACAGCATC GCTCCTATGT GGATGTGTTC 
ATCAAGACCT ACTATTTTGC GCCCGAACAG TTCGAGCAAT GGATTGAACA GCAGCGTACG 
GGCGATGAAT ACTCTCTCAA GCAATTGAAC AACCTTATTC ATTGTATTTG TGTCAGCGAT 
AAACGCACGC GACAAAGGTT GCTACAGTTG CTTGACGCTG GAATACAAAA TGGAATAGCA 
ATGTCAACGA CGCCGACAAC AACACCGCAG AAGAACAACC ACGGATCCGT AGCCGGCTCA 
AATTTAAGCA GCGTTATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611258.2
CDS	79..2979
Translation	MQNSKAKMDEFKSKFLDILHKQTNRQMKIPAMGFSDYFIQTVTTDASASQETDATDAAATSSSESNAQKSDQEILESIEQCYYNPDSNPQLYELKKVLNDGIDNELIEVTIAQLRTQQKVLTKQVLQNILEQRNACSSEFAAINDTQKKLEESLWTCQKARSYLNFARTNLTTTSLEILASYRKREVLKEVLDTLVAIKRLRTTDVEVQKLLADHNYSAAIALLLQCKSSAEAYMQFNCVQSLSKKLQETMLLMEFQLDTVLNEMVLGFDGRKYAKLQEAYKLANKSLMAMDQLHINYISAIHSSVNAVLRGYSDPGQDDQAKPLYEQLCEQLSVDKLLPCLISLCKTFWTILASYYQVVMWHNNYKLYGQEEEDSPDIYIQQKLKKGQSRVWNDILTKVCLFLQSAKLTALKYDQFIQVLSVVQRLKKVGLEFCGEQSEKLIETMQQQSEEFFRRYHICCIEEICLFLDNESWTQVDSFAHILQLPEFRSVRHTLRRHKSPTTALLASSNNSPISNNNCDELVSVHSQDGGSSIYGSYGYFLRFSEKSSPFDGGLDAAMLEEDILSGIVDEASCYFSEESDDEQKSLQSKEFDDASNQLLVNNTALNVMRVIGRYLQMCKLLHCISPKIVACILELIDFYVYAVHEIFGKDAPVATDNMHSPRLEQRLRSVEVNVLPQIKLWPLNFSSLANNELANPDTLYGLSQRIVAVEAGRSMLQQFNVLQHYLNHLLPLAERPILASYLDYSDYIADLAKLVYTCVTSRVIDLSSILAQMAKVKWDVNHVIHQHSNYSDVLNRNIQSFAMCLEEIAKEVPVPSKQVWNSMAHVATHLLVEGFSNVKKCSAGGRALMQLDFANFMSFLELISNQKYPQHRSYVDVFIKTYYFAPEQFEQWIEQQRTGDEYSLKQLNNLIHCICVSDKRTRQRLLQLLDAGIQNGIAMSTTPTTTPQKNNHGSVAGSNLSSVI

Target ORF information:

RefSeq Version	NM_137414.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 50 (Vps50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137414.3

ORF Insert Sequence:

ATGCAGAATT CCAAGGCCAA AATGGACGAA TTCAAGAGCA AATTCTTGGA CATATTGCAC 
AAACAGACGA ACCGTCAAAT GAAAATTCCT GCCATGGGTT TTTCGGATTA TTTCATTCAA 
ACGGTGACCA CAGACGCCTC CGCCAGTCAA GAAACTGATG CCACAGACGC GGCAGCCACT 
TCTTCCAGCG AGTCAAATGC ACAAAAGTCG GACCAGGAGA TCCTGGAGAG CATCGAACAG 
TGCTACTACA ACCCCGACAG CAATCCACAG CTGTACGAAC TCAAGAAAGT TCTCAACGAT 
GGCATAGACA ACGAGCTCAT CGAGGTCACC ATTGCACAAT TGCGCACTCA GCAGAAGGTT 
CTTACCAAAC AGGTGCTCCA GAACATCCTG GAGCAGCGGA ATGCCTGCAG CAGTGAGTTT 
GCCGCTATCA ATGACACGCA GAAGAAGTTG GAGGAATCGC TGTGGACATG CCAAAAGGCG 
AGATCCTATT TAAACTTTGC TCGCACGAAC CTAACAACCA CTAGCCTGGA GATCTTGGCC 
AGCTACCGCA AACGAGAGGT GCTCAAGGAG GTGTTGGACA CCCTGGTGGC CATCAAGAGA 
CTGCGCACTA CTGACGTGGA GGTACAGAAG CTGCTAGCGG ATCACAACTA CAGCGCCGCC 
ATAGCTCTGC TCCTGCAGTG CAAGAGCTCC GCCGAGGCCT ACATGCAGTT CAACTGTGTA 
CAATCGCTCT CTAAAAAGCT GCAGGAAACC ATGCTGCTTA TGGAATTTCA GCTGGATACT 
GTGCTTAATG AAATGGTGCT AGGTTTTGAT GGGCGAAAAT ACGCCAAACT GCAGGAGGCC 
TACAAATTGG CAAACAAGTC ACTGATGGCC ATGGACCAGC TGCACATCAA CTATATTTCG 
GCCATTCACT CTTCTGTGAA TGCAGTGCTT CGCGGTTACA GCGATCCTGG CCAGGACGAT 
CAGGCCAAGC CGCTCTATGA GCAGCTGTGC GAACAGCTTA GCGTGGATAA GCTGCTGCCC 
TGTCTGATTA GTTTGTGCAA AACCTTCTGG ACCATTCTGG CCTCCTACTA TCAGGTTGTT 
ATGTGGCACA ATAACTACAA GTTGTATGGA CAGGAGGAGG AGGATTCACC AGATATTTAC 
ATTCAGCAGA AACTGAAGAA GGGACAGTCA CGCGTTTGGA ATGACATTCT GACCAAAGTG 
TGCTTGTTCC TGCAGTCGGC AAAGTTGACG GCTCTTAAAT ACGATCAGTT CATCCAAGTG 
CTGTCCGTGG TACAAAGACT TAAGAAGGTG GGTCTGGAGT TCTGCGGCGA GCAGTCGGAG 
AAGTTGATCG AGACGATGCA GCAGCAGAGC GAGGAGTTCT TCCGGCGTTA CCATATCTGC 
TGCATTGAGG AAATCTGTTT ATTCTTGGAT AACGAGTCCT GGACGCAAGT CGACTCATTT 
GCGCATATCC TGCAATTGCC TGAATTCCGA TCGGTTCGGC ACACTCTTCG GCGCCACAAG 
TCTCCGACAA CTGCTTTGCT GGCCTCATCC AACAACTCCC CGATCAGCAA TAATAACTGC 
GATGAGCTGG TTTCCGTGCA CTCCCAGGAT GGTGGCAGCT CCATTTACGG TTCCTACGGA 
TATTTTCTCA GATTCTCCGA AAAAAGTTCG CCATTTGATG GTGGCTTGGA TGCAGCAATG 
TTGGAGGAGG ACATCTTATC CGGCATTGTG GACGAGGCCT CCTGTTATTT CAGCGAGGAG 
AGCGATGACG AGCAGAAAAG CTTGCAAAGC AAGGAGTTCG ATGACGCCAG CAACCAGCTG 
CTGGTAAACA ACACTGCGCT AAATGTGATG CGCGTCATTG GAAGATATCT GCAGATGTGC 
AAGTTATTGC ACTGCATCTC TCCCAAGATT GTGGCTTGCA TCCTGGAGCT CATTGACTTT 
TATGTCTACG CAGTGCACGA AATCTTCGGA AAAGATGCGC CGGTAGCAAC GGATAATATG 
CACTCTCCTA GACTGGAGCA ACGTTTACGC TCGGTCGAGG TTAACGTTCT GCCACAAATC 
AAGCTATGGC CCCTTAATTT CTCCTCTCTG GCAAATAATG AGCTAGCTAA TCCGGATACC 
TTATACGGAC TGTCACAACG CATCGTGGCC GTGGAAGCGG GTCGCAGCAT GCTTCAGCAG 
TTCAACGTCC TTCAGCACTA TCTGAACCAC TTGCTGCCCC TTGCAGAGCG TCCAATCCTG 
GCCAGTTACC TGGACTATAG CGATTATATT GCGGACTTGG CTAAACTCGT TTACACATGC 
GTCACGTCGC GCGTCATCGA TTTGTCATCC ATTCTGGCTC AGATGGCCAA GGTCAAATGG 
GATGTTAATC ATGTGATACA CCAGCACAGC AACTACAGTG ATGTTCTAAA TAGGAACATA 
CAAAGCTTCG CCATGTGTCT GGAGGAAATT GCCAAGGAGG TTCCAGTGCC CAGCAAGCAA 
GTGTGGAATT CAATGGCCCA TGTGGCCACG CATCTGCTGG TGGAGGGGTT CTCCAATGTG 
AAGAAATGCT CTGCCGGAGG ACGAGCGCTT ATGCAACTGG ATTTTGCGAA CTTTATGTCG 
TTCCTAGAGC TCATATCGAA CCAAAAGTAC CCACAGCATC GCTCCTATGT GGATGTGTTC 
ATCAAGACCT ACTATTTTGC GCCCGAACAG TTCGAGCAAT GGATTGAACA GCAGCGTACG 
GGCGATGAAT ACTCTCTCAA GCAATTGAAC AACCTTATTC ATTGTATTTG TGTCAGCGAT 
AAACGCACGC GACAAAGGTT GCTACAGTTG CTTGACGCTG GAATACAAAA TGGAATAGCA 
ATGTCAACGA CGCCGACAAC AACACCGCAG AAGAACAACC ACGGATCCGT AGCCGGCTCA 
AATTTAAGCA GCGTTATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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