Dhx36 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx36
Entrez Gene ID	72162
Full Name	DEAH (Asp-Glu-Ala-His) box polypeptide 36
Synonyms	2810407E23Rik,AI452301,AU022184,Ddx36,mKIAA1488
General protein information	Preferred Names DEAH (Asp-Glu-Ala-His) box polypeptide 36 Names ATP-dependent RNA helicase DHX36 DEAH box protein 36 MLE-like protein 1 RNA helicase associated with AU-rich element ARE probable ATP-dependent RNA helicase DHX36
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_028136.2	NP_082412.2	ATP-dependent DNA/RNA helicase DHX36
XM_030252839.1	XP_030108699.1	ATP-dependent DNA/RNA helicase DHX36 isoform X1
XM_011240240.2	XP_011238542.1	ATP-dependent RNA helicase DHX36 isoform X2
XM_011240238.1	XP_011238540.1	ATP-dependent RNA helicase DHX36 isoform X1
XM_011240238.2	XP_011238540.1	ATP-dependent DNA/RNA helicase DHX36 isoform X1
XM_011240240.3	XP_011238542.1	ATP-dependent DNA/RNA helicase DHX36 isoform X2
XM_011240242.2	XP_011238544.1	ATP-dependent RNA helicase DHX36 isoform X3
XM_030252840.1	XP_030108700.1	ATP-dependent DNA/RNA helicase DHX36 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX36	NP_065916.2
Arabidopsis thaliana (thale cress)	AT2G35920	NP_850255.1
Arabidopsis thaliana (thale cress)	AT1G48650	NP_175298.2
Bos taurus (cattle)	DHX36	NP_001073720.1
Rattus norvegicus (Norway rat)	Dhx36	NP_001101148.1
Drosophila melanogaster (fruit fly)	CG9323	NP_001260639.1
Arabidopsis thaliana (thale cress)	AT2G01130	NP_178223.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008239	XP_317231.4
Xenopus tropicalis (tropical clawed frog)	dhx36	XP_002933292.1
Mus musculus (house mouse)	Dhx36	NP_082412.2
Arabidopsis thaliana (thale cress)	AT5G04895	NP_680142.2
Macaca mulatta (Rhesus monkey)	DHX36	XP_001106252.1
Gallus gallus (chicken)	DHX36	XP_422834.3
Danio rerio (zebrafish)	si:dkey-119o24.1	NP_001122016.1
Pan troglodytes (chimpanzee)	DHX36	XP_001147599.1
Canis lupus familiaris (dog)	DHX36	XP_534311.3

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.
Nie J, Jiang M, Zhang X, Tang H, Jin H, Huang X, Yuan B, Zhang C, Lai JC, Nagamine Y, Pan D, Wang W, Yang Z
Cell reports13(4)723-732(2015 Oct)

A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.
Gao X, Ma W, Nie J, Zhang C, Zhang J, Yao G, Han J, Xu J, Hu B, Du Y, Shi Q, Yang Z, Huang X, Zhang Y
Cell death & disease6e1610(2015 Jan)

The DEAH-box helicase RHAU is an essential gene and critical for mouse hematopoiesis.
Lai JC, Ponti S, Pan D, Kohler H, Skoda RC, Matthias P, Nagamine Y
Blood119(18)4291-300(2012 May)

Histone deacetylase inhibitor MS-275 stimulates bone formation in part by enhancing Dhx36-mediated TNAP transcription.
Kim HN, Lee JH, Bae SC, Ryoo HM, Kim HH, Ha H, Lee ZH
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(9)2161-73(2011 Sep)

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Zhang Z, Kim T, Bao M, Facchinetti V, Jung SY, Ghaffari AA, Qin J, Cheng G, Liu YJ
Immunity34(6)866-78(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dhx36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu70861	XM_011240240.3 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu70862	XM_030252840.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu70862	XM_011240242.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu22135	NM_028136.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu22135	XM_030252839.1 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu22135	XM_011240238.2 Latest version!	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu22135	XM_011240238.1	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu70861	XM_011240240.2	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx36 ( XM_011240240.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238542.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu70861
Clone ID Related Accession (Same CDS sequence)	XM_011240240.2 , XM_011240240.3
Accession Version	XM_011240240.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX36 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATAG TACTGGATAC CAGATTCGTC TTCAAAGTCG GTTGCCAAGG AAACAAGGTT 
CTATCTTATA CTGCACAACA GGAATCATTC TTCAGTGGCT CCAGTCCGAC TCGTGAACGT 
TTGTCCAGTG TTAGTCATAT TGTACTTGAT GAAATTCATG AAAGGAATCT ACAGTCGGAT 
GTTTTAATGA CTGTTATTAA AGATCTTCTC CATTTTCGAT CTGATCTCAA AGTAATATTG 
ATGAGTGCAA CTTTGAATGC TGAGAAATTT TCAGAATATT TTGGTAACTG TCCAATGATA 
CATATACCCG GGTTTACTTT TCCAGTTGTG GAATATCTTT TGGAAGATAT CATTGAAAAA 
ATAAGATATG TTCCAGACCA AAAAGAACAT AGATCCCAGT TCAAGAGGGG TTTCATGCAG 
GGTCATGTAA ATAGACAAGA AAAAGAAGAA AAAGAGGCCA TCTATAAGGA ACGCTGGCCA 
GCGTATATAA AGGAACTGCG GACAAGATAC TCTGCAAGTA CCGTAGATGT TTTGCAAATG 
ATGGATGATG ATAAAGTTGA TCTGAATTTG ATTGCTGCCC TTATTCGATA CATTGTTTTG 
GAAGAAGAGG ATGGTGCAAT ATTGGTCTTT CTACCAGGCT GGGACAATAT CAGTACTTTG 
CATGATCTCT TGATGTCACA AGTGATGTTT AAATCAGATA AGTTTCTCAT TATACCTTTA 
CATTCACTGA TGCCTACCGT CAACCAGACA CAGGTATTTA AAAAAACTCC TCCCGGTGTT 
CGGAAAATAG TAATTGCTAC CAACATTGCA GAGACTAGCA TCACCATAGA TGATGTGGTT 
TATGTAATAG ATGGAGGAAA AATTAAAGAA ACACACTTTG ACACACAGAA CAACATCAGT 
ACCATGTCTG CTGAGTGGGT TAGTAAAGCT AATGCCAAAC AGAGGAAAGG GAGAGCTGGA 
AGAGTTCAGC CTGGTCATTG TTATCATCTG TATAATGGTC TTAGAGCAAG TCTTCTAGAT 
GACTACCAAC TACCAGAAAT TTTGAGAACT CCTTTGGAAG AACTCTGTTT GCAAATAAAG 
ATCTTGAGGC TGGGTGGAAT TGCTTATTTT CTGAGTAGAT TAATGGATCC ACCATCAAAT 
GAGGCAGTGG TGCTCTCCAT AAAACACCTA ATGGAACTGA GTGCTTTGGA TAAGCAAGAA 
GAATTAACAC CTCTTGGTGT CCATTTAGCC CGACTGCCTG TTGAGCCACA TATTGGAAAA 
ATGATTCTCT TTGGAGCTTT GTTCTGCTGC TTAGATCCAG TTCTCACCAT TGCTGCCAGT 
CTCAGCTTTA AAGATCCCTT TGTCATTCCA TTGGGAAAAG AAAAGATTGC AGATGCAAGA 
AGAAAAGAAC TGGCAAAGGA AACTAGAAGT GATCACCTGA CAGTTGTGAA TGCATTTGAG 
GGCTGGGAAG AAGCCAAACG ACGTGGTTTC AGGTATGAAA AGGACTACTG CTGGGAATAT 
TTTCTGTCTT CGAACACACT ACAGATGCTG CATAACATGA AGGGACAGTT TGCTGAGCAT 
CTTCTTGGAG CTGGATTTGT AAGCAGTAGA AGTCCCAAAG ATCCAAAAGC TAATATAAAT 
TCAGATAATG AGAAGATAAT TAAAGCTGTC ATCTGTGCTG GTTTATATCC CAAAGTTGCT 
AAAATCCGAC TAAATTTGGG TAAAAAGAGG AAAATGGTAA AAGTTCACAC GAAGTCTGAC 
GGCCTGGTTT CTATTCATCC TAAGTCTGTC AACGTGGAGC AGACAGACTT CCACTACAAC 
TGGCTTATCT ATCACCTGAA GATGAGAACA AGCAGTATAT ACTTGTACGA CTGTACAGAA 
GTGTCACCAT ACTGCCTCCT GTTCTTTGGA GGAGATATTT CCATCCAGAA AGATAAGGAT 
CAGGAAATTA TTGCTGTAGA TGAGTGGATT GTGTTTCAGT CTCCAGAAAG AATTGCCCAT 
CTTGTTAAGG GACTAAGAAA GGAACTGGAT AGCCTTCTAC AAGAGAAGAT TGAAAGCCCT 
CATCCTGTAG ACTGGGACGA CACTAAGTCA AGAGACTGTG CAGTACTGTC AGCTATTCTA 
GACTTGATCA AAACTCAAGA AAAGGCTACT CCAAGGAACT TGCCACCACG GTCACAGGAC 
GGATATTATA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238542.1
CDS	616..2790
Translation	MVIVLDTRFVFKVGCQGNKVLSYTAQQESFFSGSSPTRERLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	XM_011240240.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240240.2

ORF Insert Sequence:

ATGGTAATAG TACTGGATAC CAGATTCGTC TTCAAAGTCG GTTGCCAAGG AAACAAGGTT 
CTATCTTATA CTGCACAACA GGAATCATTC TTCAGTGGCT CCAGTCCGAC TCGTGAACGT 
TTGTCCAGTG TTAGTCATAT TGTACTTGAT GAAATTCATG AAAGGAATCT ACAGTCGGAT 
GTTTTAATGA CTGTTATTAA AGATCTTCTC CATTTTCGAT CTGATCTCAA AGTAATATTG 
ATGAGTGCAA CTTTGAATGC TGAGAAATTT TCAGAATATT TTGGTAACTG TCCAATGATA 
CATATACCCG GGTTTACTTT TCCAGTTGTG GAATATCTTT TGGAAGATAT CATTGAAAAA 
ATAAGATATG TTCCAGACCA AAAAGAACAT AGATCCCAGT TCAAGAGGGG TTTCATGCAG 
GGTCATGTAA ATAGACAAGA AAAAGAAGAA AAAGAGGCCA TCTATAAGGA ACGCTGGCCA 
GCGTATATAA AGGAACTGCG GACAAGATAC TCTGCAAGTA CCGTAGATGT TTTGCAAATG 
ATGGATGATG ATAAAGTTGA TCTGAATTTG ATTGCTGCCC TTATTCGATA CATTGTTTTG 
GAAGAAGAGG ATGGTGCAAT ATTGGTCTTT CTACCAGGCT GGGACAATAT CAGTACTTTG 
CATGATCTCT TGATGTCACA AGTGATGTTT AAATCAGATA AGTTTCTCAT TATACCTTTA 
CATTCACTGA TGCCTACCGT CAACCAGACA CAGGTATTTA AAAAAACTCC TCCCGGTGTT 
CGGAAAATAG TAATTGCTAC CAACATTGCA GAGACTAGCA TCACCATAGA TGATGTGGTT 
TATGTAATAG ATGGAGGAAA AATTAAAGAA ACACACTTTG ACACACAGAA CAACATCAGT 
ACCATGTCTG CTGAGTGGGT TAGTAAAGCT AATGCCAAAC AGAGGAAAGG GAGAGCTGGA 
AGAGTTCAGC CTGGTCATTG TTATCATCTG TATAATGGTC TTAGAGCAAG TCTTCTAGAT 
GACTACCAAC TACCAGAAAT TTTGAGAACT CCTTTGGAAG AACTCTGTTT GCAAATAAAG 
ATCTTGAGGC TGGGTGGAAT TGCTTATTTT CTGAGTAGAT TAATGGATCC ACCATCAAAT 
GAGGCAGTGG TGCTCTCCAT AAAACACCTA ATGGAACTGA GTGCTTTGGA TAAGCAAGAA 
GAATTAACAC CTCTTGGTGT CCATTTAGCC CGACTGCCTG TTGAGCCACA TATTGGAAAA 
ATGATTCTCT TTGGAGCTTT GTTCTGCTGC TTAGATCCAG TTCTCACCAT TGCTGCCAGT 
CTCAGCTTTA AAGATCCCTT TGTCATTCCA TTGGGAAAAG AAAAGATTGC AGATGCAAGA 
AGAAAAGAAC TGGCAAAGGA AACTAGAAGT GATCACCTGA CAGTTGTGAA TGCATTTGAG 
GGCTGGGAAG AAGCCAAACG ACGTGGTTTC AGGTATGAAA AGGACTACTG CTGGGAATAT 
TTTCTGTCTT CGAACACACT ACAGATGCTG CATAACATGA AGGGACAGTT TGCTGAGCAT 
CTTCTTGGAG CTGGATTTGT AAGCAGTAGA AGTCCCAAAG ATCCAAAAGC TAATATAAAT 
TCAGATAATG AGAAGATAAT TAAAGCTGTC ATCTGTGCTG GTTTATATCC CAAAGTTGCT 
AAAATCCGAC TAAATTTGGG TAAAAAGAGG AAAATGGTAA AAGTTCACAC GAAGTCTGAC 
GGCCTGGTTT CTATTCATCC TAAGTCTGTC AACGTGGAGC AGACAGACTT CCACTACAAC 
TGGCTTATCT ATCACCTGAA GATGAGAACA AGCAGTATAT ACTTGTACGA CTGTACAGAA 
GTGTCACCAT ACTGCCTCCT GTTCTTTGGA GGAGATATTT CCATCCAGAA AGATAAGGAT 
CAGGAAATTA TTGCTGTAGA TGAGTGGATT GTGTTTCAGT CTCCAGAAAG AATTGCCCAT 
CTTGTTAAGG GACTAAGAAA GGAACTGGAT AGCCTTCTAC AAGAGAAGAT TGAAAGCCCT 
CATCCTGTAG ACTGGGACGA CACTAAGTCA AGAGACTGTG CAGTACTGTC AGCTATTCTA 
GACTTGATCA AAACTCAAGA AAAGGCTACT CCAAGGAACT TGCCACCACG GTCACAGGAC 
GGATATTATA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_011240240.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238542.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu70861
Clone ID Related Accession (Same CDS sequence)	XM_011240240.2 , XM_011240240.3
Accession Version	XM_011240240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATAG TACTGGATAC CAGATTCGTC TTCAAAGTCG GTTGCCAAGG AAACAAGGTT 
CTATCTTATA CTGCACAACA GGAATCATTC TTCAGTGGCT CCAGTCCGAC TCGTGAACGT 
TTGTCCAGTG TTAGTCATAT TGTACTTGAT GAAATTCATG AAAGGAATCT ACAGTCGGAT 
GTTTTAATGA CTGTTATTAA AGATCTTCTC CATTTTCGAT CTGATCTCAA AGTAATATTG 
ATGAGTGCAA CTTTGAATGC TGAGAAATTT TCAGAATATT TTGGTAACTG TCCAATGATA 
CATATACCCG GGTTTACTTT TCCAGTTGTG GAATATCTTT TGGAAGATAT CATTGAAAAA 
ATAAGATATG TTCCAGACCA AAAAGAACAT AGATCCCAGT TCAAGAGGGG TTTCATGCAG 
GGTCATGTAA ATAGACAAGA AAAAGAAGAA AAAGAGGCCA TCTATAAGGA ACGCTGGCCA 
GCGTATATAA AGGAACTGCG GACAAGATAC TCTGCAAGTA CCGTAGATGT TTTGCAAATG 
ATGGATGATG ATAAAGTTGA TCTGAATTTG ATTGCTGCCC TTATTCGATA CATTGTTTTG 
GAAGAAGAGG ATGGTGCAAT ATTGGTCTTT CTACCAGGCT GGGACAATAT CAGTACTTTG 
CATGATCTCT TGATGTCACA AGTGATGTTT AAATCAGATA AGTTTCTCAT TATACCTTTA 
CATTCACTGA TGCCTACCGT CAACCAGACA CAGGTATTTA AAAAAACTCC TCCCGGTGTT 
CGGAAAATAG TAATTGCTAC CAACATTGCA GAGACTAGCA TCACCATAGA TGATGTGGTT 
TATGTAATAG ATGGAGGAAA AATTAAAGAA ACACACTTTG ACACACAGAA CAACATCAGT 
ACCATGTCTG CTGAGTGGGT TAGTAAAGCT AATGCCAAAC AGAGGAAAGG GAGAGCTGGA 
AGAGTTCAGC CTGGTCATTG TTATCATCTG TATAATGGTC TTAGAGCAAG TCTTCTAGAT 
GACTACCAAC TACCAGAAAT TTTGAGAACT CCTTTGGAAG AACTCTGTTT GCAAATAAAG 
ATCTTGAGGC TGGGTGGAAT TGCTTATTTT CTGAGTAGAT TAATGGATCC ACCATCAAAT 
GAGGCAGTGG TGCTCTCCAT AAAACACCTA ATGGAACTGA GTGCTTTGGA TAAGCAAGAA 
GAATTAACAC CTCTTGGTGT CCATTTAGCC CGACTGCCTG TTGAGCCACA TATTGGAAAA 
ATGATTCTCT TTGGAGCTTT GTTCTGCTGC TTAGATCCAG TTCTCACCAT TGCTGCCAGT 
CTCAGCTTTA AAGATCCCTT TGTCATTCCA TTGGGAAAAG AAAAGATTGC AGATGCAAGA 
AGAAAAGAAC TGGCAAAGGA AACTAGAAGT GATCACCTGA CAGTTGTGAA TGCATTTGAG 
GGCTGGGAAG AAGCCAAACG ACGTGGTTTC AGGTATGAAA AGGACTACTG CTGGGAATAT 
TTTCTGTCTT CGAACACACT ACAGATGCTG CATAACATGA AGGGACAGTT TGCTGAGCAT 
CTTCTTGGAG CTGGATTTGT AAGCAGTAGA AGTCCCAAAG ATCCAAAAGC TAATATAAAT 
TCAGATAATG AGAAGATAAT TAAAGCTGTC ATCTGTGCTG GTTTATATCC CAAAGTTGCT 
AAAATCCGAC TAAATTTGGG TAAAAAGAGG AAAATGGTAA AAGTTCACAC GAAGTCTGAC 
GGCCTGGTTT CTATTCATCC TAAGTCTGTC AACGTGGAGC AGACAGACTT CCACTACAAC 
TGGCTTATCT ATCACCTGAA GATGAGAACA AGCAGTATAT ACTTGTACGA CTGTACAGAA 
GTGTCACCAT ACTGCCTCCT GTTCTTTGGA GGAGATATTT CCATCCAGAA AGATAAGGAT 
CAGGAAATTA TTGCTGTAGA TGAGTGGATT GTGTTTCAGT CTCCAGAAAG AATTGCCCAT 
CTTGTTAAGG GACTAAGAAA GGAACTGGAT AGCCTTCTAC AAGAGAAGAT TGAAAGCCCT 
CATCCTGTAG ACTGGGACGA CACTAAGTCA AGAGACTGTG CAGTACTGTC AGCTATTCTA 
GACTTGATCA AAACTCAAGA AAAGGCTACT CCAAGGAACT TGCCACCACG GTCACAGGAC 
GGATATTATA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238542.1
CDS	645..2819
Translation	MVIVLDTRFVFKVGCQGNKVLSYTAQQESFFSGSSPTRERLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	XM_011240240.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240240.3

ORF Insert Sequence:

ATGGTAATAG TACTGGATAC CAGATTCGTC TTCAAAGTCG GTTGCCAAGG AAACAAGGTT 
CTATCTTATA CTGCACAACA GGAATCATTC TTCAGTGGCT CCAGTCCGAC TCGTGAACGT 
TTGTCCAGTG TTAGTCATAT TGTACTTGAT GAAATTCATG AAAGGAATCT ACAGTCGGAT 
GTTTTAATGA CTGTTATTAA AGATCTTCTC CATTTTCGAT CTGATCTCAA AGTAATATTG 
ATGAGTGCAA CTTTGAATGC TGAGAAATTT TCAGAATATT TTGGTAACTG TCCAATGATA 
CATATACCCG GGTTTACTTT TCCAGTTGTG GAATATCTTT TGGAAGATAT CATTGAAAAA 
ATAAGATATG TTCCAGACCA AAAAGAACAT AGATCCCAGT TCAAGAGGGG TTTCATGCAG 
GGTCATGTAA ATAGACAAGA AAAAGAAGAA AAAGAGGCCA TCTATAAGGA ACGCTGGCCA 
GCGTATATAA AGGAACTGCG GACAAGATAC TCTGCAAGTA CCGTAGATGT TTTGCAAATG 
ATGGATGATG ATAAAGTTGA TCTGAATTTG ATTGCTGCCC TTATTCGATA CATTGTTTTG 
GAAGAAGAGG ATGGTGCAAT ATTGGTCTTT CTACCAGGCT GGGACAATAT CAGTACTTTG 
CATGATCTCT TGATGTCACA AGTGATGTTT AAATCAGATA AGTTTCTCAT TATACCTTTA 
CATTCACTGA TGCCTACCGT CAACCAGACA CAGGTATTTA AAAAAACTCC TCCCGGTGTT 
CGGAAAATAG TAATTGCTAC CAACATTGCA GAGACTAGCA TCACCATAGA TGATGTGGTT 
TATGTAATAG ATGGAGGAAA AATTAAAGAA ACACACTTTG ACACACAGAA CAACATCAGT 
ACCATGTCTG CTGAGTGGGT TAGTAAAGCT AATGCCAAAC AGAGGAAAGG GAGAGCTGGA 
AGAGTTCAGC CTGGTCATTG TTATCATCTG TATAATGGTC TTAGAGCAAG TCTTCTAGAT 
GACTACCAAC TACCAGAAAT TTTGAGAACT CCTTTGGAAG AACTCTGTTT GCAAATAAAG 
ATCTTGAGGC TGGGTGGAAT TGCTTATTTT CTGAGTAGAT TAATGGATCC ACCATCAAAT 
GAGGCAGTGG TGCTCTCCAT AAAACACCTA ATGGAACTGA GTGCTTTGGA TAAGCAAGAA 
GAATTAACAC CTCTTGGTGT CCATTTAGCC CGACTGCCTG TTGAGCCACA TATTGGAAAA 
ATGATTCTCT TTGGAGCTTT GTTCTGCTGC TTAGATCCAG TTCTCACCAT TGCTGCCAGT 
CTCAGCTTTA AAGATCCCTT TGTCATTCCA TTGGGAAAAG AAAAGATTGC AGATGCAAGA 
AGAAAAGAAC TGGCAAAGGA AACTAGAAGT GATCACCTGA CAGTTGTGAA TGCATTTGAG 
GGCTGGGAAG AAGCCAAACG ACGTGGTTTC AGGTATGAAA AGGACTACTG CTGGGAATAT 
TTTCTGTCTT CGAACACACT ACAGATGCTG CATAACATGA AGGGACAGTT TGCTGAGCAT 
CTTCTTGGAG CTGGATTTGT AAGCAGTAGA AGTCCCAAAG ATCCAAAAGC TAATATAAAT 
TCAGATAATG AGAAGATAAT TAAAGCTGTC ATCTGTGCTG GTTTATATCC CAAAGTTGCT 
AAAATCCGAC TAAATTTGGG TAAAAAGAGG AAAATGGTAA AAGTTCACAC GAAGTCTGAC 
GGCCTGGTTT CTATTCATCC TAAGTCTGTC AACGTGGAGC AGACAGACTT CCACTACAAC 
TGGCTTATCT ATCACCTGAA GATGAGAACA AGCAGTATAT ACTTGTACGA CTGTACAGAA 
GTGTCACCAT ACTGCCTCCT GTTCTTTGGA GGAGATATTT CCATCCAGAA AGATAAGGAT 
CAGGAAATTA TTGCTGTAGA TGAGTGGATT GTGTTTCAGT CTCCAGAAAG AATTGCCCAT 
CTTGTTAAGG GACTAAGAAA GGAACTGGAT AGCCTTCTAC AAGAGAAGAT TGAAAGCCCT 
CATCCTGTAG ACTGGGACGA CACTAAGTCA AGAGACTGTG CAGTACTGTC AGCTATTCTA 
GACTTGATCA AAACTCAAGA AAAGGCTACT CCAAGGAACT TGCCACCACG GTCACAGGAC 
GGATATTATA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_011240242.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238544.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.

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CloneID	OMu70862
Clone ID Related Accession (Same CDS sequence)	XM_011240242.2 , XM_030252840.1
Accession Version	XM_011240242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX36 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238544.1
CDS	94..1890
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGR

Target ORF information:

RefSeq Version	XM_011240242.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240242.2

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_030252840.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108700.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X4, mRNA.

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CloneID	OMu70862
Clone ID Related Accession (Same CDS sequence)	XM_011240242.2 , XM_030252840.1
Accession Version	XM_030252840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108700.1
CDS	96..1892
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGR

Target ORF information:

RefSeq Version	XM_030252840.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252840.1

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( NM_028136.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082412.2 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), mRNA.

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CloneID	OMu22135
Clone ID Related Accession (Same CDS sequence)	NM_028136.2 , XM_030252839.1 , XM_011240238.1 , XM_011240238.2
Accession Version	NM_028136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA/RNA helicase DHX36
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF448804.1 and AC114424.10. On Jun 20, 2009 this sequence version replaced NM_028136.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF448804.1, BC138061.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082412.2
CDS	50..3055
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	NM_028136.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028136.2

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_030252839.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108699.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu22135
Clone ID Related Accession (Same CDS sequence)	NM_028136.2 , XM_030252839.1 , XM_011240238.1 , XM_011240238.2
Accession Version	XM_030252839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108699.1
CDS	50..3055
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	XM_030252839.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252839.1

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_011240238.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238540.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22135
Clone ID Related Accession (Same CDS sequence)	NM_028136.2 , XM_030252839.1 , XM_011240238.1 , XM_011240238.2
Accession Version	XM_011240238.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ATP-dependent RNA helicase DHX36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238540.1
CDS	90..3095
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	XM_011240238.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240238.1

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_011240238.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238540.1 Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.

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CloneID	OMu22135
Clone ID Related Accession (Same CDS sequence)	NM_028136.2 , XM_030252839.1 , XM_011240238.1 , XM_011240238.2
Accession Version	XM_011240238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ATP-dependent DNA/RNA helicase DHX36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238540.1
CDS	92..3097
Translation	MSYDYHQSWSRDGGPRGSGQGSSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIKTQEKATPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	XM_011240238.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240238.2

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGATGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCCAGCG GCGGCGGCGG CGGAGGGAGC CGGGGCTCGG GCGGCGGCGG AGGGGGCCGC 
GGCGGCCGGG GCCGGCATCC CGCACACCTT AAGGGTCGCG AGATCGGCCT GTGGTACGCC 
AAGAAGCAGA CGCAGAAGAA CAAGGAGGCT GAGAGGCAGG AGAGAGCTGT AGTGCACATG 
GATGAACGTC GAGAAGAGCA AATTGTGCAG CTGCTGAATT CAGTCCAAGC TAAGACTGAC 
AAAGATTCAG AAGCGCAGAT ATCCTGGTTT GCTCCTGAGG ATCATGGGTA TGGTACTGAA 
GTTTCTTCAG AGAAAAAAAT AAACTCAGAG AAGAAACTTG ACAACCAGGA AAAGAAATTG 
CTAAACCAAG AAAAAAAGAC ATTTAGGATC ACAGACAAAT CATATATTGA CCGAGATTCT 
GAGTATTTAT TGCAAGAAAA TGAGCCAAAC CTAAGCTTAG ATCAACATTT ACTGGAAGAT 
TTACAAAGGA AAAAAACTGA CCCTCGATAT ATAGAGATGC AGCGTTTCAG AAAAAAGCTG 
CCTTCATATG GAATGCAGAA GGAGCTGGTA AATCTAATCA ATAACCATCA GGTGACAGTA 
ATAAGTGGTG AAACTGGTTG TGGCAAAACC ACTCAGGTTA CGCAGTTCAT CTTGGATAAC 
TACATCGAAA GAGGAAAAGG GTCTGCCTGC AGAATAGTGT GTACTCAGCC GAGAAGAATT 
AGTGCCATTT CAGTCGCTGA GAGAGTGGCC ACAGAAAGGG CAGAGTCTTG TGGCAATGGT 
AATAGTACTG GATACCAGAT TCGTCTTCAA AGTCGGTTGC CAAGGAAACA AGGTTCTATC 
TTATACTGCA CAACAGGAAT CATTCTTCAG TGGCTCCAGT CCGACTCACG TTTGTCCAGT 
GTTAGTCATA TTGTACTTGA TGAAATTCAT GAAAGGAATC TACAGTCGGA TGTTTTAATG 
ACTGTTATTA AAGATCTTCT CCATTTTCGA TCTGATCTCA AAGTAATATT GATGAGTGCA 
ACTTTGAATG CTGAGAAATT TTCAGAATAT TTTGGTAACT GTCCAATGAT ACATATACCC 
GGGTTTACTT TTCCAGTTGT GGAATATCTT TTGGAAGATA TCATTGAAAA AATAAGATAT 
GTTCCAGACC AAAAAGAACA TAGATCCCAG TTCAAGAGGG GTTTCATGCA GGGTCATGTA 
AATAGACAAG AAAAAGAAGA AAAAGAGGCC ATCTATAAGG AACGCTGGCC AGCGTATATA 
AAGGAACTGC GGACAAGATA CTCTGCAAGT ACCGTAGATG TTTTGCAAAT GATGGATGAT 
GATAAAGTTG ATCTGAATTT GATTGCTGCC CTTATTCGAT ACATTGTTTT GGAAGAAGAG 
GATGGTGCAA TATTGGTCTT TCTACCAGGC TGGGACAATA TCAGTACTTT GCATGATCTC 
TTGATGTCAC AAGTGATGTT TAAATCAGAT AAGTTTCTCA TTATACCTTT ACATTCACTG 
ATGCCTACCG TCAACCAGAC ACAGGTATTT AAAAAAACTC CTCCCGGTGT TCGGAAAATA 
GTAATTGCTA CCAACATTGC AGAGACTAGC ATCACCATAG ATGATGTGGT TTATGTAATA 
GATGGAGGAA AAATTAAAGA AACACACTTT GACACACAGA ACAACATCAG TACCATGTCT 
GCTGAGTGGG TTAGTAAAGC TAATGCCAAA CAGAGGAAAG GGAGAGCTGG AAGAGTTCAG 
CCTGGTCATT GTTATCATCT GTATAATGGT CTTAGAGCAA GTCTTCTAGA TGACTACCAA 
CTACCAGAAA TTTTGAGAAC TCCTTTGGAA GAACTCTGTT TGCAAATAAA GATCTTGAGG 
CTGGGTGGAA TTGCTTATTT TCTGAGTAGA TTAATGGATC CACCATCAAA TGAGGCAGTG 
GTGCTCTCCA TAAAACACCT AATGGAACTG AGTGCTTTGG ATAAGCAAGA AGAATTAACA 
CCTCTTGGTG TCCATTTAGC CCGACTGCCT GTTGAGCCAC ATATTGGAAA AATGATTCTC 
TTTGGAGCTT TGTTCTGCTG CTTAGATCCA GTTCTCACCA TTGCTGCCAG TCTCAGCTTT 
AAAGATCCCT TTGTCATTCC ATTGGGAAAA GAAAAGATTG CAGATGCAAG AAGAAAAGAA 
CTGGCAAAGG AAACTAGAAG TGATCACCTG ACAGTTGTGA ATGCATTTGA GGGCTGGGAA 
GAAGCCAAAC GACGTGGTTT CAGGTATGAA AAGGACTACT GCTGGGAATA TTTTCTGTCT 
TCGAACACAC TACAGATGCT GCATAACATG AAGGGACAGT TTGCTGAGCA TCTTCTTGGA 
GCTGGATTTG TAAGCAGTAG AAGTCCCAAA GATCCAAAAG CTAATATAAA TTCAGATAAT 
GAGAAGATAA TTAAAGCTGT CATCTGTGCT GGTTTATATC CCAAAGTTGC TAAAATCCGA 
CTAAATTTGG GTAAAAAGAG GAAAATGGTA AAAGTTCACA CGAAGTCTGA CGGCCTGGTT 
TCTATTCATC CTAAGTCTGT CAACGTGGAG CAGACAGACT TCCACTACAA CTGGCTTATC 
TATCACCTGA AGATGAGAAC AAGCAGTATA TACTTGTACG ACTGTACAGA AGTGTCACCA 
TACTGCCTCC TGTTCTTTGG AGGAGATATT TCCATCCAGA AAGATAAGGA TCAGGAAATT 
ATTGCTGTAG ATGAGTGGAT TGTGTTTCAG TCTCCAGAAA GAATTGCCCA TCTTGTTAAG 
GGACTAAGAA AGGAACTGGA TAGCCTTCTA CAAGAGAAGA TTGAAAGCCC TCATCCTGTA 
GACTGGGACG ACACTAAGTC AAGAGACTGT GCAGTACTGT CAGCTATTCT AGACTTGATC 
AAAACTCAAG AAAAGGCTAC TCCAAGGAAC TTGCCACCAC GGTCACAGGA CGGATATTAT 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.
Cell reports13(4)723-732(2015 Oct)
Nie J,Jiang M,Zhang X,Tang H,Jin H,Huang X,Yuan B,Zhang C,Lai JC,Nagamine Y,Pan D,Wang W,Yang Z

A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.
Cell death & disease6e1610(2015 Jan)
Gao X,Ma W,Nie J,Zhang C,Zhang J,Yao G,Han J,Xu J,Hu B,Du Y,Shi Q,Yang Z,Huang X,Zhang Y

The DEAH-box helicase RHAU is an essential gene and critical for mouse hematopoiesis.
Blood119(18)4291-300(2012 May)
Lai JC,Ponti S,Pan D,Kohler H,Skoda RC,Matthias P,Nagamine Y

Histone deacetylase inhibitor MS-275 stimulates bone formation in part by enhancing Dhx36-mediated TNAP transcription.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(9)2161-73(2011 Sep)
Kim HN,Lee JH,Bae SC,Ryoo HM,Kim HH,Ha H,Lee ZH

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Immunity34(6)866-78(2011 Jun)
Zhang Z,Kim T,Bao M,Facchinetti V,Jung SY,Ghaffari AA,Qin J,Cheng G,Liu YJ

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Dhx36 cDNA ORF clone, Mus musculus(house mouse)

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