WLS cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	WLS
Entrez Gene ID	68151
Full Name	wntless WNT ligand secretion mediator
Synonyms	5031439A09Rik,AI173978,AI987742,EVI,Gpr177
General protein information	Preferred Names wntless WNT ligand secretion mediator Names protein wntless homolog G protein-coupled receptor 177 integral membrane protein GPR177 protein evenness interrupted homolog wntless homolog
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H4

mRNA and Protein(s)

mRNA	Protein	Name
NM_026582.5	NP_080858.3	protein wntless homolog isoform 1 precursor
NM_001356349.1	NP_001343278.1	protein wntless homolog isoform 2 precursor
NM_001356350.1	NP_001343279.1	protein wntless homolog isoform 1 precursor
XM_006501964.1	XP_006502027.1	protein wntless homolog isoform X1
XM_006501964.2	XP_006502027.1	protein wntless homolog isoform X1
NM_026582.4	NP_080858.3	protein wntless homolog precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wls	NP_080858.3
Homo sapiens (human)	WLS	NP_079187.3
Macaca mulatta (Rhesus monkey)	WLS	XP_001095839.2
Gallus gallus (chicken)	WLS	NP_001026465.1
Danio rerio (zebrafish)	wls	NP_998311.2
Bos taurus (cattle)	WLS	NP_001179580.1
Rattus norvegicus (Norway rat)	Wls	NP_955440.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007896	XP_001237950.2
Pan troglodytes (chimpanzee)	WLS	XP_001164915.2
Xenopus tropicalis (tropical clawed frog)	wls	NP_001016910.1
Caenorhabditis elegans (roundworm)	mig-14	NP_001022275.1
Canis lupus familiaris (dog)	WLS	XP_853835.1
Drosophila melanogaster (fruit fly)	wls	NP_648445.1

Gene Ontology
Bibliography

Related articles in PubMed

Antagonistic interactions between osterix and pyrophosphate during cementum formation.
Choi H, Liu Y, Jeong JK, Kim TH, Cho ES
Bone1258-15(2019 Aug)

Inhibiting Glutamine-Dependent mTORC1 Activation Ameliorates Liver Cancers Driven by β-Catenin Mutations.
Adebayo Michael AO, Ko S, Tao J, Moghe A, Yang H, Xu M, Russell JO, Pradhan-Sundd T, Liu S, Singh S, Poddar M, Monga JS, Liu P, Oertel M, Ranganathan S, Singhi A, Rebouissou S, Zucman-Rossi J, Ribback S, Calvisi D, Qvartskhava N, Görg B, Häussinger D, Chen X, Monga SP
Cell metabolism29(5)1135-1150.e6(2019 May)

A Role for Sensory end Organ-Derived Signals in Regulating Muscle Spindle Proprioceptor Phenotype.
Wu D, Schieren I, Qian Y, Zhang C, Jessell TM, de Nooij JC
The Journal of neuroscience : the official journal of the Society for Neuroscience39(22)4252-4267(2019 May)

WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis.
Kaiser K, Gyllborg D, Procházka J, Salašová A, Kompaníková P, Molina FL, Laguna-Goya R, Radaszkiewicz T, Harnoš J, Procházková M, Potěšil D, Barker RA, Casado ÁG, Zdráhal Z, Sedláček R, Arenas E, Villaescusa JC, Bryja V
Nature communications10(1)1498(2019 04)

Wnt/Fgf crosstalk is required for the specification of basal cells in the mouse trachea.
Hou Z, Wu Q, Sun X, Chen H, Li Y, Zhang Y, Mori M, Yang Y, Que J, Jiang M
Development (Cambridge, England)146(3)(2019 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19005	XM_006501964.2 Latest version!	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OMu83642	NM_001356349.1 Latest version!	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu19005	NM_001356350.1 Latest version!	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00
OMu19005	NM_026582.5 Latest version!	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00
View Old Accession Versions >>
OMu19005	XM_006501964.1	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OMu19005	NM_026582.4	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WLS ( NM_026582.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_080858.3 Mus musculus wntless homolog (Drosophila) (Wls), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19005
Clone ID Related Accession (Same CDS sequence)	NM_026582.5 , NM_001356350.1 , XM_006501964.1 , XM_006501964.2 , NM_026582.4
Accession Version	NM_026582.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	protein wntless homolog precursor
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075615.1 and CF352837.1. On Nov 23, 2010 this sequence version replaced NM_026582.3. Transcript Variant: This variant (1) represents the shorter, protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075615.1, AK029053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080858.3
CDS	248..1873
Translation	MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	NM_026582.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026582.4

ORF Insert Sequence:

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WLS ( XM_006501964.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006502027.1 Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19005
Clone ID Related Accession (Same CDS sequence)	NM_026582.5 , NM_001356350.1 , XM_006501964.1 , XM_006501964.2 , NM_026582.4
Accession Version	XM_006501964.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423324800000
Organism	Mus musculus(House mouse)
Product	protein wntless homolog isoform X1
Comment

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006502027.1

Translation

MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	XM_006501964.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501964.1

ORF Insert Sequence:

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WLS ( XM_006501964.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006502027.1 Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19005
Clone ID Related Accession (Same CDS sequence)	NM_026582.5 , NM_001356350.1 , XM_006501964.1 , XM_006501964.2 , NM_026582.4
Accession Version	XM_006501964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	protein wntless homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502027.1
CDS	211..1836
Translation	MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	XM_006501964.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus wntless homolog (Drosophila) (Wls), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501964.2

ORF Insert Sequence:

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WLS ( NM_001356349.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001343278.1 Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 2, mRNA.

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CloneID	OMu83642
Clone ID Related Accession (Same CDS sequence)	NM_001356349.1
Accession Version	NM_001356349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572192000000
Organism	Mus musculus(house mouse)
Product	protein wntless homolog isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121906.3, BQ179316.1, AK019886.1, AC175030.2 and AC225719.3. On Sep 30, 2017 this sequence version replaced NR_037590.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK019886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343278.1
CDS	674..1999
Translation	MEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	NM_001356349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356349.1

ORF Insert Sequence:

ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WLS ( NM_026582.5 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_080858.3 Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 1, mRNA.

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CloneID	OMu19005
Clone ID Related Accession (Same CDS sequence)	NM_026582.5 , NM_001356350.1 , XM_006501964.1 , XM_006501964.2 , NM_026582.4
Accession Version	NM_026582.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572192000000
Organism	Mus musculus(house mouse)
Product	protein wntless homolog isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121906.3, BC075615.1, AC175030.2 and AC225719.3. On Sep 30, 2017 this sequence version replaced NM_026582.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029053.1, SRR1660819.54493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080858.3
CDS	293..1918
Translation	MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	NM_026582.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026582.5

ORF Insert Sequence:

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WLS ( NM_001356350.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001343279.1 Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu19005
Clone ID Related Accession (Same CDS sequence)	NM_026582.5 , NM_001356350.1 , XM_006501964.1 , XM_006501964.2 , NM_026582.4
Accession Version	NM_001356350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572192000000
Organism	Mus musculus(house mouse)
Product	protein wntless homolog isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121906.3, AK044810.1, AC175245.2 and AC225719.3. On Sep 30, 2017 this sequence version replaced XM_006501964.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.156310.1, AK044810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343279.1
CDS	293..1918
Translation	MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVPYTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSMEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE

Target ORF information:

RefSeq Version	NM_001356350.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus wntless WNT ligand secretion mediator (Wls), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356350.1

ORF Insert Sequence:

ATGGCTGGGG CAATTATAGA AAACATGAGT ACCAAGAAGC TCTGCATTGT TGGAGGGATT 
CTTCTGGTTT TCCAAATCGT TGCCTTTCTG GTGGGAGGCT TGATCGCTCC AGCACCCACA 
ACAGCAGTAC CCTACACGGC AATAAAATGT GTGGATGTCC GTAAGAACCA CCATAAAACA 
AGATGGCTGG CGCCTTGGGG ACCTAACAAG TGTGACAAGA TCCGTGACAT CGAGGAAGCA 
ATTCCAAGGG AAATTGAAGC AAATGACATT GTGTTTTCTG TACACATTCC CCTCCCTTCT 
ATGGAGATGA GTCCATGGTT CCAGTTTATG CTGTTTATCT TGCAGCTAGA CATTGCTTTC 
AAGCTGAACA ACCAAATCAG AGAAAATGCA GAAATTTCCA TGGATGTTTC CCTGGGTTAC 
CGTGATGATA TGTTTTCTGA ATGGACTGAA ATGGCCCATG AAAGAGTACC ACGTAAACTC 
AAATGTACTT TTACATCCCC CAAGACCCCA GAGCACGAAG GTCGTTATTA TAATTGTGAT 
GTCCTTCCTT TCATGGAAAT TGGGTCAGTG GCTCATAAGT ATTACCTTCT AAATATCCGG 
CTACCTGTAA ATGAGAAAAA GAAAATCAAT GTTGGAATTG GGGAGATAAA GGACATTCGG 
TTGGTGGGAA TCCACCAAAA CGGAGGTTTC ACTAAGGTAT GGTTTGCTAT GAAGACCTTC 
CTTACACCCA GCATCTTCAT CATTATGGTG TGGTATTGGA GGAGGATCAC CATGATGTCC 
CGACCTCCAG TGCTTCTGGA AAAAGTCATC TTTGCCCTTG GGATTTCCAT GACCTTTATC 
AATATCCCTG TGGAATGGTT TTCTATTGGA TTTGACTGGA CCTGGATGCT GTTATTTGGT 
GACATCCGAC AGGGCATCTT CTATGCAATG CTTCTTTCTT TCTGGATCAT CTTCTGTGGC 
GAGCACATGA TGGATCAACA TGAACGGAAT CACATTGCAG GGTACTGGAA GCAAGTTGGA 
CCAATTGCTG TTGGCTCCTT CTGCCTCTTC ATATTTGACA TGTGTGAAAG AGGAGTACAA 
CTCACAAATC CTTTCTACAG TATCTGGACT ACAGATGTTG GAACAGAACT GGCTATGGCT 
TTCATCATTG TGGCAGGTAT CTGCCTCTGC CTCTATTTCC TGTTTCTGTG CTTCATGGTA 
TTTCAGGTAT TTAGAAACAT CAGTGGGAAG CAGTCTAGCC TCCCAGCCAT GAGCAAAGTC 
CGGAGGCTGC ACTATGAGGG TCTGATCTTC AGGTTCAAGT TCCTCATGCT TATCACCTTG 
GCTTGTGCTG CCATGACTGT TATCTTCTTC ATCGTTAGTC AGGTGTCAGA AGGCCATTGG 
AAATGGGGTG GTGTCACAGT TCAAGTGAGC AGTGCTTTCT TCACTGGAAT CTATGGGATG 
TGGAACCTGT ATGTCTTTGC TTTGATGTTC TTGTATGCAC CATCCCATAA GAACTATGGG 
GAAGACCAGT CTAATGGTGA CCTGGGTGTC CACAGCGGGG AAGAACTGCA GCTTACTACC 
ACTATAACCC ATGTAGATGG ACCAACTGAG ATCTACAAGT TGACCCGTAA AGAAGCACAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antagonistic interactions between osterix and pyrophosphate during cementum formation.
Bone1258-15(2019 Aug)
Choi H,Liu Y,Jeong JK,Kim TH,Cho ES

Inhibiting Glutamine-Dependent mTORC1 Activation Ameliorates Liver Cancers Driven by β-Catenin Mutations.
Cell metabolism29(5)1135-1150.e6(2019 May)
Adebayo Michael AO,Ko S,Tao J,Moghe A,Yang H,Xu M,Russell JO,Pradhan-Sundd T,Liu S,Singh S,Poddar M,Monga JS,Liu P,Oertel M,Ranganathan S,Singhi A,Rebouissou S,Zucman-Rossi J,Ribback S,Calvisi D,Qvartskhava N,Görg B,Häussinger D,Chen X,Monga SP

A Role for Sensory end Organ-Derived Signals in Regulating Muscle Spindle Proprioceptor Phenotype.
The Journal of neuroscience : the official journal of the Society for Neuroscience39(22)4252-4267(2019 May)
Wu D,Schieren I,Qian Y,Zhang C,Jessell TM,de Nooij JC

WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis.
Nature communications10(1)1498(2019 04)
Kaiser K,Gyllborg D,Procházka J,Salašová A,Kompaníková P,Molina FL,Laguna-Goya R,Radaszkiewicz T,Harnoš J,Procházková M,Potěšil D,Barker RA,Casado ÁG,Zdráhal Z,Sedláček R,Arenas E,Villaescusa JC,Bryja V

Wnt/Fgf crosstalk is required for the specification of basal cells in the mouse trachea.
Development (Cambridge, England)146(3)(2019 02)
Hou Z,Wu Q,Sun X,Chen H,Li Y,Zhang Y,Mori M,Yang Y,Que J,Jiang M

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WLS cDNA ORF clone, Mus musculus(House mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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