Ttll4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttll4
Entrez Gene ID	67534
Full Name	tubulin tyrosine ligase-like family, member 4
Synonyms	4632407P03Rik,AI451681,mKIAA0173
General protein information	Preferred Names tubulin tyrosine ligase-like family, member 4 Names tubulin polyglutamylase TTLL4 polyglutamylase tubulin--tyrosine ligase-like protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496224.2	XP_006496287.1	tubulin polyglutamylase TTLL4 isoform X1
XM_006496224.3	XP_006496287.1	tubulin polyglutamylase TTLL4 isoform X1
XM_006496225.2	XP_006496288.1	tubulin polyglutamylase TTLL4 isoform X1
NM_001014974.1	NP_001014974.1	tubulin polyglutamylase TTLL4
NM_001014974.2	NP_001014974.1	tubulin polyglutamylase TTLL4

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ttll-4	NP_001022985.1
Rattus norvegicus (Norway rat)	Ttll4	XP_006245360.1
Homo sapiens (human)	TTLL4	NP_055455.3
Pan troglodytes (chimpanzee)	TTLL4	XP_001159133.1
Macaca mulatta (Rhesus monkey)	TTLL4	XP_001094864.1
Bos taurus (cattle)	TTLL4	NP_001192637.1
Mus musculus (house mouse)	Ttll4	NP_001014974.1
Xenopus tropicalis (tropical clawed frog)	ttll4	XP_002933990.2
Canis lupus familiaris (dog)	TTLL4	XP_545645.2
Gallus gallus (chicken)	TTLL4	XP_422058.4

Gene Ontology
Bibliography

Related articles in PubMed

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.
Ye B, Liu B, Hao L, Zhu X, Yang L, Wang S, Xia P, Du Y, Meng S, Huang G, Qin X, Wang Y, Yan X, Li C, Hao J, Zhu P, He L, Tian Y, Fan Z
Nature communications9(1)1261(2018 03)

IL-7Rα glutamylation and activation of transcription factor Sall3 promote group 3 ILC development.
Liu B, Ye B, Zhu X, Huang G, Yang L, Zhu P, Du Y, Wu J, Meng S, Tian Y, Fan Z
Nature communications8(1)231(2017 08)

Glutamylation of the DNA sensor cGAS regulates its binding and synthase activity in antiviral immunity.
Xia P, Ye B, Wang S, Zhu X, Du Y, Xiong Z, Tian Y, Fan Z
Nature immunology17(4)369-78(2016 Apr)

Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia.
Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C
The Journal of cell biology202(3)441-51(2013 Aug)

A family of protein-deglutamylating enzymes associated with neurodegeneration.
Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme JC, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin MJ, Janke C
Cell143(4)564-78(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ttll4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttll4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21670	NM_001014974.2 Latest version!	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$643.30 $919.00
OMu21670	XM_006496225.2 Latest version!	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$643.30 $919.00
OMu21670	XM_006496224.3 Latest version!	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$643.30 $919.00
View Old Accession Versions >>
OMu21670	XM_006496224.2	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$643.30 $919.00
OMu21670	NM_001014974.1	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttll4 ( NM_001014974.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001014974.1 Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.

Price & Availability↑

CloneID	OMu21670
Clone ID Related Accession (Same CDS sequence)	XM_006496224.2 , XM_006496224.3 , XM_006496225.2 , NM_001014974.1 , NM_001014974.2
Accession Version	NM_001014974.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1546012800000
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase TTLL4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085151.1. On May 17, 2005 this sequence version replaced XM_129771.7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014974.1
CDS	398..3979
Translation	MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS

Target ORF information:

RefSeq Version	NM_001014974.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014974.1

ORF Insert Sequence:

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttll4 ( NM_001014974.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001014974.1 Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.

Price & Availability↑

CloneID	OMu21670
Clone ID Related Accession (Same CDS sequence)	XM_006496224.2 , XM_006496224.3 , XM_006496225.2 , NM_001014974.1 , NM_001014974.2
Accession Version	NM_001014974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase TTLL4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152409.8. On Feb 2, 2019 this sequence version replaced NM_001014974.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085151.1, SRR5314785.133244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014974.1
CDS	450..4031
Translation	MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS

Target ORF information:

RefSeq Version	NM_001014974.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014974.2

ORF Insert Sequence:

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttll4 ( XM_006496224.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496287.1 Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.

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CloneID	OMu21670
Clone ID Related Accession (Same CDS sequence)	XM_006496224.2 , XM_006496224.3 , XM_006496225.2 , NM_001014974.1 , NM_001014974.2
Accession Version	XM_006496224.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase TTLL4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496224.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496287.1
CDS	374..3955
Translation	MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS

Target ORF information:

RefSeq Version	XM_006496224.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496224.2

ORF Insert Sequence:

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttll4 ( XM_006496224.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496287.1 Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.

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CloneID	OMu21670
Clone ID Related Accession (Same CDS sequence)	XM_006496224.2 , XM_006496224.3 , XM_006496225.2 , NM_001014974.1 , NM_001014974.2
Accession Version	XM_006496224.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase TTLL4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496287.1
CDS	625..4206
Translation	MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS

Target ORF information:

RefSeq Version	XM_006496224.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496224.3

ORF Insert Sequence:

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttll4 ( XM_006496225.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496288.1 Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X2, mRNA.

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CloneID	OMu21670
Clone ID Related Accession (Same CDS sequence)	XM_006496224.2 , XM_006496224.3 , XM_006496225.2 , NM_001014974.1 , NM_001014974.2
Accession Version	XM_006496225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	tubulin polyglutamylase TTLL4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496288.1
CDS	340..3921
Translation	MASAGTEHYSIGLRRGNSFKQRHPSGTVSASPSEKPSEVKVWSQAHQQVKPIWKLEKKHVGTLSAGLGTSFLGVPSQPAYFLCPSTLCSSGTTAVIAGHSNPCYLQSLPNLFSNTLLYRRTNVRQKPYQQLESFCLRSSPSEKRSFSLPQKGLPVSVTANKATSSTVFPMAQPMATSPTDPYLSLAAAGENPSRKSLASAISGKIASPLSYKPMLNNNSFMRPNSTKVPLSQATDGLKPVSSPKIQPVSWHHSGGTGDCVPQPGDHKVPQNIATVLDDVTAPITPSIPSTLNISTASVTSSQCSQSNFRMEAHPCGLDENPDSQSATKEVHFTEAVRKLAEKGLEKMPRQGYQFEQACFVNPSFQWGLLNRSRRWKPLMGQRFPQEDIGLDSAILPGTSDTLGLDSTVFCTKRISIHLLASHVHGLNPSPACGSAVDPQVLGEDRAPVPPSSLQPLGVAEVATRLSSVHLDQPGKEPEEAKDLNSCTKGGGSATDLQPNQVEPEDTEDELGDGLEDSCSHDENEEEEGDSECSSLSVVSPSESVALISRNCVDLMSKSLPNHEKVVRPALIYSLFPNVTPTIYFGTRDERVEKLPWEQRRLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPGFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALKALWNYLSQKGINSDAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPNMEDIISSSSSPSSSSGSSTSLPSSPRDKCQMTPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRVLVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRNWCYKGFHTGIVSDSAPLWSLPTSLMTTSKGDGTPNSASKSRKKSASEGTTLSSEDRSTPKSKKSQAGLSPISRKTLSSRSNENTSKQSKRSTPGLPVLKYSGQSSRLSAASASQSVTDSRLTAVSS

Target ORF information:

RefSeq Version	XM_006496225.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase-like family, member 4 (Ttll4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496225.2

ORF Insert Sequence:

ATGGCCTCAG CAGGAACAGA GCACTACAGT ATTGGCCTGC GCCGAGGAAA CAGCTTCAAG 
CAGAGACATC CCTCAGGCAC AGTGTCTGCT TCACCATCTG AGAAACCCTC GGAGGTCAAA 
GTCTGGTCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AACTGGAAAA GAAGCACGTA 
GGCACACTGT CAGCAGGGCT GGGCACCAGT TTCTTGGGAG TCCCATCCCA GCCAGCCTAT 
TTCCTTTGCC CTAGCACTTT ATGTAGCTCT GGGACCACAG CAGTCATTGC AGGCCACAGC 
AACCCCTGTT ACCTGCAGTC CCTCCCTAAC TTGTTCAGCA ATACTCTGCT GTACCGCCGC 
ACCAACGTGA GGCAAAAACC ATACCAGCAA CTGGAATCTT TCTGCTTGCG TTCCAGCCCT 
TCAGAGAAAA GATCTTTTTC TCTCCCTCAG AAGGGTCTTC CTGTCAGTGT CACTGCCAAT 
AAAGCCACAT CTTCTACAGT CTTCCCCATG GCCCAGCCCA TGGCAACTTC ACCCACTGAT 
CCATACCTCT CACTGGCAGC AGCTGGGGAG AACCCTTCAA GGAAGAGCCT GGCCTCTGCT 
ATCTCAGGGA AGATTGCATC TCCACTGTCC TATAAGCCCA TGCTGAACAA CAACTCCTTC 
ATGAGGCCAA ATAGCACTAA AGTGCCCTTA TCACAAGCCA CAGATGGCCT GAAGCCAGTA 
TCCTCACCCA AGATCCAGCC TGTCTCATGG CATCATTCAG GGGGTACTGG GGACTGTGTA 
CCCCAACCTG GTGACCATAA GGTGCCCCAA AACATTGCTA CTGTCTTAGA TGATGTCACT 
GCCCCTATCA CCCCATCTAT CCCTAGCACC CTCAACATAT CCACAGCCAG TGTTACCTCT 
TCCCAGTGCA GTCAGAGTAA CTTTAGGATG GAGGCACATC CCTGTGGCCT GGATGAAAAC 
CCGGATTCCC AGAGTGCTAC TAAAGAGGTA CACTTCACTG AGGCTGTGAG GAAGTTGGCT 
GAAAAAGGCT TGGAGAAGAT GCCACGACAA GGCTACCAGT TTGAACAGGC TTGTTTTGTG 
AACCCCAGCT TCCAGTGGGG TCTTCTCAAC AGAAGCAGGC GGTGGAAACC TCTGATGGGC 
CAGCGGTTTC CTCAGGAGGA TATTGGATTA GACAGTGCGA TCCTTCCTGG TACTTCAGAC 
ACCTTGGGGT TGGACAGCAC AGTCTTCTGT ACCAAGAGGA TCAGCATTCA CCTCCTTGCC 
TCACATGTCC ATGGGCTCAA TCCCAGCCCT GCCTGTGGGT CTGCAGTTGA CCCCCAGGTA 
CTTGGAGAAG ACAGAGCTCC TGTTCCCCCT TCTTCCCTCC AGCCTCTTGG TGTAGCTGAA 
GTGGCCACTC GTCTTTCTTC TGTCCATCTT GACCAGCCTG GGAAGGAGCC TGAGGAAGCC 
AAGGACCTGA ACTCTTGTAC TAAGGGTGGT GGTTCAGCTA CTGACCTCCA GCCAAACCAG 
GTGGAGCCTG AAGATACAGA AGATGAACTG GGAGATGGTT TGGAAGATAG CTGCAGCCAT 
GATGAGAATG AAGAAGAGGA AGGAGACTCA GAGTGCTCTT CTTTAAGTGT TGTCTCCCCC 
AGCGAGTCAG TGGCATTAAT ATCTCGGAAC TGTGTGGATT TGATGTCCAA ATCCCTTCCC 
AATCATGAGA AAGTTGTACG GCCAGCCCTC ATCTACAGTC TCTTCCCCAA TGTAACCCCT 
ACCATCTATT TTGGGACTCG GGATGAAAGA GTGGAGAAAC TTCCTTGGGA ACAGAGGAGG 
CTGCTTCGGT GGAAGATGAG CACAGTGACC CCCAACATTG TCAAGCAGAC CATCGGTCGG 
TCACACTTCA AAATCAGCAA GAGAAATGAT GACTGGCTGG GCTGCTGGGG TCACCACATG 
AAGTCTCCTG GTTTTCGATC CATTAGAGAG CATCAGAAGC TAAACCACTT CCCAGGCTCC 
TTCCAGATTG GGAGGAAGGA CCGGCTGTGG CGGAACCTGT CTCGCATGCA AAGCCGTTTT 
GGCAAGAAGG AGTTCAGTTT CTTTCCTCAG TCCTTTATCC TGCCCCAGGA TTCCAAGCTC 
TTGCGCAAAG CCTGGGAGAG CAGCAGCCGC CAGAAGTGGA TTGTGAAGCC ACCAGCATCT 
GCTCGAGGCA TTGGCATTCA GGTCATTCAC AAATGGAGTC AGCTCCCCAA GCGCAGGCCC 
CTCCTGGTAC AGAGGTATCT ACACAAGCCC TACCTCATTA GTGGCAGCAA ATTTGATCTT 
CGGATCTATG TTTACGTGAC TTCTTATGAT CCTCTACGGA TTTACCTCTT TTCGGATGGA 
CTCGTCCGTT TTGCCAGTTG CAAGTATTCC CCTTCCATGA AGAGTCTTAG CAACAAGTTC 
ATGCACCTGA CCAACTACAG TGTCAATAAA AAGAACACAG AATACCAGGC TAATGCTGAT 
GAGACTGCAT GCCAAGGGCA CAAATGGGCA TTGAAAGCTT TGTGGAACTA TCTGAGCCAG 
AAAGGAATTA ATAGTGATGC CATCTGGGAG AAGATAAAGG ATGTTGTAGT CAAAACCATC 
ATCTCGTCAG AGCCCTATGT GACTAACCTA CTGAAGTTAT ATGTGCGGCG CCCCTATAGC 
TGTCATGAGC TCTTTGGTTT TGACATCATG CTGGATGAGA ACCTGAAACC CTGGGTCCTA 
GAAGTCAACA TTTCTCCAAG CCTCCATTCC AACTCTCCTC TGGACATCAG CATCAAAGGC 
CAGATGATCC GAGACCTTCT GAACCTGGCT GGCTTTGTTC TGCCCAACAT GGAGGATATC 
ATCTCTAGTT CCAGCAGCCC CAGCAGCTCC AGCGGCTCCA GCACCAGCTT GCCAAGCTCT 
CCTAGAGACA AATGTCAAAT GACTCCAGAG CACTTCACTG CACAGAAGAT GAAGAAAGCC 
TATTACTTGA CCCAGAAAAT TCCTGACCAG GACTTCTATG CGTCCGTGCT GGACGTCTTG 
ACACCAGATG ATGTTCGGGT TTTGGTGGAG ATGGAAGATG AGTTTTCTCG TCGTGGTCAA 
TTTGAAAGAA TTTTTCCTTC CCGAATCTCT TCTCGATATC TTCGGTTTTT TGAGCAGCCA 
CGATATTTCA ACATTCTCAC CACCCAGTGG GAACAGAAGT ACCATGGAAA CAAGCTCAAA 
GGAGTAGATC TGCTTCGGAA TTGGTGCTAC AAAGGCTTCC ACACGGGGAT TGTCTCTGAC 
TCAGCCCCAC TGTGGTCTCT GCCAACATCG CTCATGACTA CCTCAAAGGG TGATGGGACA 
CCAAATTCCG CCAGCAAGTC ACGAAAGAAG AGCGCCTCTG AGGGAACCAC ACTGTCCTCT 
GAAGACAGGT CAACACCCAA GTCTAAGAAG AGTCAAGCTG GCCTGTCTCC CATATCCAGG 
AAGACTTTGT CCTCAAGGAG CAATGAGAAC ACCAGCAAAC AGTCCAAGCG CTCTACCCCG 
GGGTTACCTG TCCTCAAGTA CTCTGGGCAG AGTTCCAGAC TTTCTGCTGC TTCTGCTTCC 
CAGTCAGTCA CTGACTCCCG CTTGACTGCT GTGAGCTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.
Nature communications9(1)1261(2018 03)
Ye B,Liu B,Hao L,Zhu X,Yang L,Wang S,Xia P,Du Y,Meng S,Huang G,Qin X,Wang Y,Yan X,Li C,Hao J,Zhu P,He L,Tian Y,Fan Z

IL-7Rα glutamylation and activation of transcription factor Sall3 promote group 3 ILC development.
Nature communications8(1)231(2017 08)
Liu B,Ye B,Zhu X,Huang G,Yang L,Zhu P,Du Y,Wu J,Meng S,Tian Y,Fan Z

Glutamylation of the DNA sensor cGAS regulates its binding and synthase activity in antiviral immunity.
Nature immunology17(4)369-78(2016 Apr)
Xia P,Ye B,Wang S,Zhu X,Du Y,Xiong Z,Tian Y,Fan Z

Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia.
The Journal of cell biology202(3)441-51(2013 Aug)
Bosch Grau M,Gonzalez Curto G,Rocha C,Magiera MM,Marques Sousa P,Giordano T,Spassky N,Janke C

A family of protein-deglutamylating enzymes associated with neurodegeneration.
Cell143(4)564-78(2010 Nov)
Rogowski K,van Dijk J,Magiera MM,Bosc C,Deloulme JC,Bosson A,Peris L,Gold ND,Lacroix B,Bosch Grau M,Bec N,Larroque C,Desagher S,Holzer M,Andrieux A,Moutin MJ,Janke C

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Ttll4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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