Trim62 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim62
Entrez Gene ID	67525
Full Name	tripartite motif-containing 62
Synonyms	6330414G21Rik,AI450348,Dear1
General protein information	Preferred Names tripartite motif-containing 62 Names E3 ubiquitin-protein ligase TRIM62 RING-type E3 ubiquitin transferase TRIM62 tripartite motif-containing protein 62
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_178110.2	NP_835211.1	E3 ubiquitin-protein ligase TRIM62
XM_006503322.3	XP_006503385.1	E3 ubiquitin-protein ligase TRIM62 isoform X1
NM_001370757.1	NP_001357686.1	E3 ubiquitin-protein ligase TRIM62 isoform 3
NM_001370756.1	NP_001357685.1	E3 ubiquitin-protein ligase TRIM62 isoform 1
NM_178110.3	NP_835211.1	E3 ubiquitin-protein ligase TRIM62 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIM62	NP_060677.2
Pan troglodytes (chimpanzee)	TRIM62	XP_003308012.1
Macaca mulatta (Rhesus monkey)	TRIM62	XP_001107247.1
Bos taurus (cattle)	TRIM62	NP_001192764.1
Mus musculus (house mouse)	Trim62	NP_835211.1
Gallus gallus (chicken)	TRIM62	XP_427363.3
Danio rerio (zebrafish)	trim62	NP_001074143.1
Rattus norvegicus (Norway rat)	Trim62	XP_232757.5

Gene Ontology
Bibliography

Related articles in PubMed

The Trim family of genes and the retina: Expression and functional characterization.
Chowdhury R, Laboissonniere LA, Wester AK, Muller M, Trimarchi JM
PloS one13(9)e0202867(2018)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Ubiquitin Ligase TRIM62 Regulates CARD9-Mediated Anti-fungal Immunity and Intestinal Inflammation.
Cao Z, Conway KL, Heath RJ, Rush JS, Leshchiner ES, Ramirez-Ortiz ZG, Nedelsky NB, Huang H, Ng A, Gardet A, Cheng SC, Shamji AF, Rioux JD, Wijmenga C, Netea MG, Means TK, Daly MJ, Xavier RJ
Immunity43(4)715-26(2015 Oct)

Loss of the novel tumour suppressor and polarity gene Trim62 (Dear1) synergizes with oncogenic Ras in invasive lung cancer.
Quintás-Cardama A, Post SM, Solis LM, Xiong S, Yang P, Chen N, Wistuba II, Killary AM, Lozano G
The Journal of pathology234(1)108-19(2014 Sep)

DEAR1 is a chromosome 1p35 tumor suppressor and master regulator of TGF-β-driven epithelial-mesenchymal transition.
Chen N, Balasenthil S, Reuther J, Frayna A, Wang Y, Chandler DS, Abruzzo LV, Rashid A, Rodriguez J, Lozano G, Cao Y, Lokken E, Chen J, Frazier ML, Sahin AA, Wistuba II, Sen S, Lott ST, Killary AM
Cancer discovery3(10)1172-89(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trim62 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim62 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84796	NM_001370757.1 Latest version!	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu84799	NM_001370756.1 Latest version!	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu33757	XM_006503322.3 Latest version!	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu07103	NM_178110.3 Latest version!	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$279.30 $399.00
View Old Accession Versions >>
OMu07103	NM_178110.2	Mus musculus tripartite motif-containing 62 (Trim62), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$279.30 $399.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim62 ( NM_001370757.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357686.1 Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84796
Clone ID Related Accession (Same CDS sequence)	NM_001370757.1
Accession Version	NM_001370757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558886400000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM62 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL607086.19. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4144706.1, SRR7345562.4535139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGTC CTCTACCAAG 
AGCCTGCGGA CCACCATCGG GGAAGCCTTT GAGCGGCTGC ACCGGCTACT TCGAGAGCGA 
CAGAAGGCCA TGCTGGAGGA ACTGGAGGCC GACACAGCCC GCACACTGAC GGACATTGAG 
CAGAAGGTCC AGCGTTACAG CCAGCAACTA CGAAAGGTGC AAGAGGGGGC CCAGATCCTG 
CAGGAGCGGC TGGCAGAGAC TGACCGCCAC ACCTTCCTTG CAGGAGTGGC CTCCCTGTCT 
GAGAGGCTCA AGGGGAAGAT TCATGAGACC AACCTAACGT ACGAAGACTT CCCGACCTCC 
AAGTACACAG GCCCCCTGCA GTACACCATC TGGAAATCGC TTTTCCAGGA CATCCATCCA 
GTGCCAGCTG CCCTGACCAT GGACCCAGGC ACTGCCCACC AGCGCCTGAT CCTGTCCGAT 
GACTGCACCA TCGTGGCCTA CGGGAACCTA CACCCACAAC CACTGCAGGA CTCACCAAAG 
CGCTTTGACG TAGAGGTGTC GGTGCTGGGC TCTGAAGCCT TCAGTAGCGG TGTCCACTAC 
TGGGAGGTGG TGGTGGCGGA GAAGACCCAG TGGGTGATTG GGCTGGCCCA TGAGGCTGCC 
AGTCGCAAAG GCAGCATCCA GATTCAGCCT AGCCGCGGCT TCTACTGCAT CGTTATGCAT 
GACGGTAACC AGTACAGCGC CTGCACAGAG CCCTGGACTA GGCTCAACGT CCGTGACAAG 
CTTGACAAGG TGGGCGTCTT CCTGGACTAT GACCAGGGCC TCCTCATCTT CTACAACGCC 
GATGACATGT CCTGGCTCTA CACCTTCCGA GAGAAGTTTC CTGGCAAGCT CTGCTCCTAC 
TTCAGCCCCG GCCAGAGCCA CGCCAATGGC AAGAACGTCC AACCACTGCG GATCAACACC 
GTCCGCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357686.1
CDS	127..1458
Translation	MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLANIVERYSAFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFEELQSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTMDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

Target ORF information:

RefSeq Version	NM_001370757.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370757.1

ORF Insert Sequence:

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGTC CTCTACCAAG 
AGCCTGCGGA CCACCATCGG GGAAGCCTTT GAGCGGCTGC ACCGGCTACT TCGAGAGCGA 
CAGAAGGCCA TGCTGGAGGA ACTGGAGGCC GACACAGCCC GCACACTGAC GGACATTGAG 
CAGAAGGTCC AGCGTTACAG CCAGCAACTA CGAAAGGTGC AAGAGGGGGC CCAGATCCTG 
CAGGAGCGGC TGGCAGAGAC TGACCGCCAC ACCTTCCTTG CAGGAGTGGC CTCCCTGTCT 
GAGAGGCTCA AGGGGAAGAT TCATGAGACC AACCTAACGT ACGAAGACTT CCCGACCTCC 
AAGTACACAG GCCCCCTGCA GTACACCATC TGGAAATCGC TTTTCCAGGA CATCCATCCA 
GTGCCAGCTG CCCTGACCAT GGACCCAGGC ACTGCCCACC AGCGCCTGAT CCTGTCCGAT 
GACTGCACCA TCGTGGCCTA CGGGAACCTA CACCCACAAC CACTGCAGGA CTCACCAAAG 
CGCTTTGACG TAGAGGTGTC GGTGCTGGGC TCTGAAGCCT TCAGTAGCGG TGTCCACTAC 
TGGGAGGTGG TGGTGGCGGA GAAGACCCAG TGGGTGATTG GGCTGGCCCA TGAGGCTGCC 
AGTCGCAAAG GCAGCATCCA GATTCAGCCT AGCCGCGGCT TCTACTGCAT CGTTATGCAT 
GACGGTAACC AGTACAGCGC CTGCACAGAG CCCTGGACTA GGCTCAACGT CCGTGACAAG 
CTTGACAAGG TGGGCGTCTT CCTGGACTAT GACCAGGGCC TCCTCATCTT CTACAACGCC 
GATGACATGT CCTGGCTCTA CACCTTCCGA GAGAAGTTTC CTGGCAAGCT CTGCTCCTAC 
TTCAGCCCCG GCCAGAGCCA CGCCAATGGC AAGAACGTCC AACCACTGCG GATCAACACC 
GTCCGCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim62 ( NM_001370756.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001357685.1 Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu84799
Clone ID Related Accession (Same CDS sequence)	NM_001370756.1
Accession Version	NM_001370756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558886400000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM62 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL607086.19. On May 28, 2019 this sequence version replaced XM_006503322.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2243933.1, SRR7974084.13495.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGGC CCTGTGTGTA 
TTTCAGAGAG AACTGAAGGA GCAGCTTCAG GCCCTTCAGG ACAGCGAGCG GGAACACACG 
GAGGCGCTGC AGCTCCTCAA GCGACAGTTG GCAGAGACCA AGTCCTCTAC CAAGAGCCTG 
CGGACCACCA TCGGGGAAGC CTTTGAGCGG CTGCACCGGC TACTTCGAGA GCGACAGAAG 
GCCATGCTGG AGGAACTGGA GGCCGACACA GCCCGCACAC TGACGGACAT TGAGCAGAAG 
GTCCAGCGTT ACAGCCAGCA ACTACGAAAG GTGCAAGAGG GGGCCCAGAT CCTGCAGGAG 
CGGCTGGCAG AGACTGACCG CCACACCTTC CTTGCAGGAG TGGCCTCCCT GTCTGAGAGG 
CTCAAGGGGA AGATTCATGA GACCAACCTA ACGTACGAAG ACTTCCCGAC CTCCAAGTAC 
ACAGGCCCCC TGCAGTACAC CATCTGGAAA TCGCTTTTCC AGGACATCCA TCCAGTGCCA 
GCTGCCCTGA CCATGGACCC AGGCACTGCC CACCAGCGCC TGATCCTGTC CGATGACTGC 
ACCATCGTGG CCTACGGGAA CCTACACCCA CAACCACTGC AGGACTCACC AAAGCGCTTT 
GACGTAGAGG TGTCGGTGCT GGGCTCTGAA GCCTTCAGTA GCGGTGTCCA CTACTGGGAG 
GTGGTGGTGG CGGAGAAGAC CCAGTGGGTG ATTGGGCTGG CCCATGAGGC TGCCAGTCGC 
AAAGGCAGCA TCCAGATTCA GCCTAGCCGC GGCTTCTACT GCATCGTTAT GCATGACGGT 
AACCAGTACA GCGCCTGCAC AGAGCCCTGG ACTAGGCTCA ACGTCCGTGA CAAGCTTGAC 
AAGGTGGGCG TCTTCCTGGA CTATGACCAG GGCCTCCTCA TCTTCTACAA CGCCGATGAC 
ATGTCCTGGC TCTACACCTT CCGAGAGAAG TTTCCTGGCA AGCTCTGCTC CTACTTCAGC 
CCCGGCCAGA GCCACGCCAA TGGCAAGAAC GTCCAACCAC TGCGGATCAA CACCGTCCGC 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357685.1
CDS	127..1572
Translation	MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLANIVERYSAFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFEELQALCVFQRELKEQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTMDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

Target ORF information:

RefSeq Version	NM_001370756.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370756.1

ORF Insert Sequence:

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGGC CCTGTGTGTA 
TTTCAGAGAG AACTGAAGGA GCAGCTTCAG GCCCTTCAGG ACAGCGAGCG GGAACACACG 
GAGGCGCTGC AGCTCCTCAA GCGACAGTTG GCAGAGACCA AGTCCTCTAC CAAGAGCCTG 
CGGACCACCA TCGGGGAAGC CTTTGAGCGG CTGCACCGGC TACTTCGAGA GCGACAGAAG 
GCCATGCTGG AGGAACTGGA GGCCGACACA GCCCGCACAC TGACGGACAT TGAGCAGAAG 
GTCCAGCGTT ACAGCCAGCA ACTACGAAAG GTGCAAGAGG GGGCCCAGAT CCTGCAGGAG 
CGGCTGGCAG AGACTGACCG CCACACCTTC CTTGCAGGAG TGGCCTCCCT GTCTGAGAGG 
CTCAAGGGGA AGATTCATGA GACCAACCTA ACGTACGAAG ACTTCCCGAC CTCCAAGTAC 
ACAGGCCCCC TGCAGTACAC CATCTGGAAA TCGCTTTTCC AGGACATCCA TCCAGTGCCA 
GCTGCCCTGA CCATGGACCC AGGCACTGCC CACCAGCGCC TGATCCTGTC CGATGACTGC 
ACCATCGTGG CCTACGGGAA CCTACACCCA CAACCACTGC AGGACTCACC AAAGCGCTTT 
GACGTAGAGG TGTCGGTGCT GGGCTCTGAA GCCTTCAGTA GCGGTGTCCA CTACTGGGAG 
GTGGTGGTGG CGGAGAAGAC CCAGTGGGTG ATTGGGCTGG CCCATGAGGC TGCCAGTCGC 
AAAGGCAGCA TCCAGATTCA GCCTAGCCGC GGCTTCTACT GCATCGTTAT GCATGACGGT 
AACCAGTACA GCGCCTGCAC AGAGCCCTGG ACTAGGCTCA ACGTCCGTGA CAAGCTTGAC 
AAGGTGGGCG TCTTCCTGGA CTATGACCAG GGCCTCCTCA TCTTCTACAA CGCCGATGAC 
ATGTCCTGGC TCTACACCTT CCGAGAGAAG TTTCCTGGCA AGCTCTGCTC CTACTTCAGC 
CCCGGCCAGA GCCACGCCAA TGGCAAGAAC GTCCAACCAC TGCGGATCAA CACCGTCCGC 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim62 ( XM_006503322.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503385.1 Mus musculus tripartite motif-containing 62 (Trim62), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33757
Clone ID Related Accession (Same CDS sequence)	XM_006503322.3
Accession Version	XM_006503322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM62 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTTA GGTGCTATCT TCTCACTTTT TCTTCATTAG CTGTCCTCTC GCCCCCTGCC 
GCCAGGACCC TAAACAAACC TGACAGCTGT GCAGACCCAG AAGAAACAGC TGTTTTTCCC 
GTCCCTGGGG GTCAGGAGCA CAGTGGCAGC GAGGCGGGGT CTGGAGGCCC CTCCCCTCCC 
CCCGCCCGCC GCGGGGGCGG AGCGGCTCGG GTCCCGCCCG CGGGGGCGGA GAGGGAGCCT 
GGGCGCCGGG CAGTTCGGCT CGGCTGGTTC CGGGTTGAGA GGCTGCGCTG GACCGAAGCG 
GTGGCCGCTG AGCTGGTGCG GGTAAGGGGC CGCGCGGGGC CGGGATCTGC GGCCAGGGTC 
GGGCAGCTGA GTGGGCCGGC ACCCCTCCTC TGCTCTGCCG GGCAAAGCCG CGGTGCGGCT 
GGGTCTGGCT GCTCTGTCCC GCAGAATTCC CCATCCCCAG CTCGACCCCC TCTCAAAGCT 
TTTCGGGTCC CGGGATTCCG CCCCTCTTCG GGATCTCCTC CTCGCAGCAT CTATCCCTTG 
GGAGTGTCTG GTCCTCTAGA TCCTCCTCGC CTGGAGCCCC TTCCTTGGGA CGCCCTCGGC 
CTCTCTCCTC CAGCGCCCAT CGCTGGTGGC CACCTAGGAC CTCGAAACCG AGCTCCGGTG 
CTCGCATCCC GGGTGGCACC CACTCGGCAC CGAGCCCTCG CGCCTCCGGC GTCGCAGACA 
GCTCCCCCCG CGCTCCCCGG CGCCTGTCGC CCGTGCCCCC GTGGGCCTGG CCCCCGGGTA 
CCGCCCCCAA TCGCGCCGCG CCGGCCGCCG GCCTCCCGGG CTCCGCTTCG CGCCGCCCCT 
GGCGCCATGG CGTGCAGCCT CAAGGACGAG CTGCTGTGCT CCATCTGCCT GAGCATCTAC 
CAGGACCCGG TGAGCCTGGG CTGCGAGCAC TACTTCTGCC GCCGCTGCAT CACCGAGCAC 
TGGGTGCGGC AGGAGGCTCA GGGCGCCCGC GACTGCCCGG AGTGCCGGCG CACGTTCGCC 
GAGCCCGCGC TCGCGCCCAG CCTCAAGCTG GCCAACATCG TGGAGCGCTA CAGCGCCTTC 
CCGCTGGACG CCATCCTCAA CGCGCGCCGA GCCGCGCGAC CCTGCCAGGC TCACGACAAG 
GTCAAGCTCT TCTGCCTCAC GGACCGCGCA CTGCTCTGCT TCTTCTGTGA TGAGCCCGCG 
CTGCACGAGC AACACCAGGT CACTGGCATC GACGATGCCT TCGAGGAGTT GCAGGCCCTG 
TGTGTATTTC AGAGAGAACT GAAGGAGCAG CTTCAGGCCC TTCAGGACAG CGAGCGGGAA 
CACACGGAGG CGCTGCAGCT CCTCAAGCGA CAGTTGGCAG AGACCAAGTC CTCTACCAAG 
AGCCTGCGGA CCACCATCGG GGAAGCCTTT GAGCGGCTGC ACCGGCTACT TCGAGAGCGA 
CAGAAGGCCA TGCTGGAGGA ACTGGAGGCC GACACAGCCC GCACACTGAC GGACATTGAG 
CAGAAGGTCC AGCGTTACAG CCAGCAACTA CGAAAGGTGC AAGAGGGGGC CCAGATCCTG 
CAGGAGCGGC TGGCAGAGAC TGACCGCCAC ACCTTCCTTG CAGGAGTGGC CTCCCTGTCT 
GAGAGGCTCA AGGGGAAGAT TCATGAGACC AACCTAACGT ACGAAGACTT CCCGACCTCC 
AAGTACACAG GCCCCCTGCA GTACACCATC TGGAAATCGC TTTTCCAGGA CATCCATCCA 
GTGCCAGCTG CCCTGACCAT GGACCCAGGC ACTGCCCACC AGCGCCTGAT CCTGTCCGAT 
GACTGCACCA TCGTGGCCTA CGGGAACCTA CACCCACAAC CACTGCAGGA CTCACCAAAG 
CGCTTTGACG TAGAGGTGTC GGTGCTGGGC TCTGAAGCCT TCAGTAGCGG TGTCCACTAC 
TGGGAGGTGG TGGTGGCGGA GAAGACCCAG TGGGTGATTG GGCTGGCCCA TGAGGCTGCC 
AGTCGCAAAG GCAGCATCCA GATTCAGCCT AGCCGCGGCT TCTACTGCAT CGTTATGCAT 
GACGGTAACC AGTACAGCGC CTGCACAGAG CCCTGGACTA GGCTCAACGT CCGTGACAAG 
CTTGACAAGG TGGGCGTCTT CCTGGACTAT GACCAGGGCC TCCTCATCTT CTACAACGCC 
GATGACATGT CCTGGCTCTA CACCTTCCGA GAGAAGTTTC CTGGCAAGCT CTGCTCCTAC 
TTCAGCCCCG GCCAGAGCCA CGCCAATGGC AAGAACGTCC AACCACTGCG GATCAACACC 
GTCCGCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503385.1
CDS	1633..3924
Translation	MFLRCYLLTFSSLAVLSPPAARTLNKPDSCADPEETAVFPVPGGQEHSGSEAGSGGPSPPPARRGGGAARVPPAGAEREPGRRAVRLGWFRVERLRWTEAVAAELVRVRGRAGPGSAARVGQLSGPAPLLCSAGQSRGAAGSGCSVPQNSPSPARPPLKAFRVPGFRPSSGSPPRSIYPLGVSGPLDPPRLEPLPWDALGLSPPAPIAGGHLGPRNRAPVLASRVAPTRHRALAPPASQTAPPALPGACRPCPRGPGPRVPPPIAPRRPPASRAPLRAAPGAMACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLANIVERYSAFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFEELQALCVFQRELKEQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTMDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

Target ORF information:

RefSeq Version	XM_006503322.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503322.3

ORF Insert Sequence:

ATGTTCCTTA GGTGCTATCT TCTCACTTTT TCTTCATTAG CTGTCCTCTC GCCCCCTGCC 
GCCAGGACCC TAAACAAACC TGACAGCTGT GCAGACCCAG AAGAAACAGC TGTTTTTCCC 
GTCCCTGGGG GTCAGGAGCA CAGTGGCAGC GAGGCGGGGT CTGGAGGCCC CTCCCCTCCC 
CCCGCCCGCC GCGGGGGCGG AGCGGCTCGG GTCCCGCCCG CGGGGGCGGA GAGGGAGCCT 
GGGCGCCGGG CAGTTCGGCT CGGCTGGTTC CGGGTTGAGA GGCTGCGCTG GACCGAAGCG 
GTGGCCGCTG AGCTGGTGCG GGTAAGGGGC CGCGCGGGGC CGGGATCTGC GGCCAGGGTC 
GGGCAGCTGA GTGGGCCGGC ACCCCTCCTC TGCTCTGCCG GGCAAAGCCG CGGTGCGGCT 
GGGTCTGGCT GCTCTGTCCC GCAGAATTCC CCATCCCCAG CTCGACCCCC TCTCAAAGCT 
TTTCGGGTCC CGGGATTCCG CCCCTCTTCG GGATCTCCTC CTCGCAGCAT CTATCCCTTG 
GGAGTGTCTG GTCCTCTAGA TCCTCCTCGC CTGGAGCCCC TTCCTTGGGA CGCCCTCGGC 
CTCTCTCCTC CAGCGCCCAT CGCTGGTGGC CACCTAGGAC CTCGAAACCG AGCTCCGGTG 
CTCGCATCCC GGGTGGCACC CACTCGGCAC CGAGCCCTCG CGCCTCCGGC GTCGCAGACA 
GCTCCCCCCG CGCTCCCCGG CGCCTGTCGC CCGTGCCCCC GTGGGCCTGG CCCCCGGGTA 
CCGCCCCCAA TCGCGCCGCG CCGGCCGCCG GCCTCCCGGG CTCCGCTTCG CGCCGCCCCT 
GGCGCCATGG CGTGCAGCCT CAAGGACGAG CTGCTGTGCT CCATCTGCCT GAGCATCTAC 
CAGGACCCGG TGAGCCTGGG CTGCGAGCAC TACTTCTGCC GCCGCTGCAT CACCGAGCAC 
TGGGTGCGGC AGGAGGCTCA GGGCGCCCGC GACTGCCCGG AGTGCCGGCG CACGTTCGCC 
GAGCCCGCGC TCGCGCCCAG CCTCAAGCTG GCCAACATCG TGGAGCGCTA CAGCGCCTTC 
CCGCTGGACG CCATCCTCAA CGCGCGCCGA GCCGCGCGAC CCTGCCAGGC TCACGACAAG 
GTCAAGCTCT TCTGCCTCAC GGACCGCGCA CTGCTCTGCT TCTTCTGTGA TGAGCCCGCG 
CTGCACGAGC AACACCAGGT CACTGGCATC GACGATGCCT TCGAGGAGTT GCAGGCCCTG 
TGTGTATTTC AGAGAGAACT GAAGGAGCAG CTTCAGGCCC TTCAGGACAG CGAGCGGGAA 
CACACGGAGG CGCTGCAGCT CCTCAAGCGA CAGTTGGCAG AGACCAAGTC CTCTACCAAG 
AGCCTGCGGA CCACCATCGG GGAAGCCTTT GAGCGGCTGC ACCGGCTACT TCGAGAGCGA 
CAGAAGGCCA TGCTGGAGGA ACTGGAGGCC GACACAGCCC GCACACTGAC GGACATTGAG 
CAGAAGGTCC AGCGTTACAG CCAGCAACTA CGAAAGGTGC AAGAGGGGGC CCAGATCCTG 
CAGGAGCGGC TGGCAGAGAC TGACCGCCAC ACCTTCCTTG CAGGAGTGGC CTCCCTGTCT 
GAGAGGCTCA AGGGGAAGAT TCATGAGACC AACCTAACGT ACGAAGACTT CCCGACCTCC 
AAGTACACAG GCCCCCTGCA GTACACCATC TGGAAATCGC TTTTCCAGGA CATCCATCCA 
GTGCCAGCTG CCCTGACCAT GGACCCAGGC ACTGCCCACC AGCGCCTGAT CCTGTCCGAT 
GACTGCACCA TCGTGGCCTA CGGGAACCTA CACCCACAAC CACTGCAGGA CTCACCAAAG 
CGCTTTGACG TAGAGGTGTC GGTGCTGGGC TCTGAAGCCT TCAGTAGCGG TGTCCACTAC 
TGGGAGGTGG TGGTGGCGGA GAAGACCCAG TGGGTGATTG GGCTGGCCCA TGAGGCTGCC 
AGTCGCAAAG GCAGCATCCA GATTCAGCCT AGCCGCGGCT TCTACTGCAT CGTTATGCAT 
GACGGTAACC AGTACAGCGC CTGCACAGAG CCCTGGACTA GGCTCAACGT CCGTGACAAG 
CTTGACAAGG TGGGCGTCTT CCTGGACTAT GACCAGGGCC TCCTCATCTT CTACAACGCC 
GATGACATGT CCTGGCTCTA CACCTTCCGA GAGAAGTTTC CTGGCAAGCT CTGCTCCTAC 
TTCAGCCCCG GCCAGAGCCA CGCCAATGGC AAGAACGTCC AACCACTGCG GATCAACACC 
GTCCGCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim62 ( NM_178110.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835211.1 Mus musculus tripartite motif-containing 62 (Trim62), mRNA.

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CloneID	OMu07103
Clone ID Related Accession (Same CDS sequence)	NM_178110.2 , NM_178110.3
Accession Version	NM_178110.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1547740800000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM62
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049095.1. On Jun 3, 2003 this sequence version replaced NM_178110.1. ##Evidence-Data-START## Transcript exon combination :: BC049095.1, CX562710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGAG AGAACTGAAG 
GAGCAGCTTC AGGCCCTTCA GGACAGCGAG CGGGAACACA CGGAGGCGCT GCAGCTCCTC 
AAGCGACAGT TGGCAGAGAC CAAGTCCTCT ACCAAGAGCC TGCGGACCAC CATCGGGGAA 
GCCTTTGAGC GGCTGCACCG GCTACTTCGA GAGCGACAGA AGGCCATGCT GGAGGAACTG 
GAGGCCGACA CAGCCCGCAC ACTGACGGAC ATTGAGCAGA AGGTCCAGCG TTACAGCCAG 
CAACTACGAA AGGTGCAAGA GGGGGCCCAG ATCCTGCAGG AGCGGCTGGC AGAGACTGAC 
CGCCACACCT TCCTTGCAGG AGTGGCCTCC CTGTCTGAGA GGCTCAAGGG GAAGATTCAT 
GAGACCAACC TAACGTACGA AGACTTCCCG ACCTCCAAGT ACACAGGCCC CCTGCAGTAC 
ACCATCTGGA AATCGCTTTT CCAGGACATC CATCCAGTGC CAGCTGCCCT GACCATGGAC 
CCAGGCACTG CCCACCAGCG CCTGATCCTG TCCGATGACT GCACCATCGT GGCCTACGGG 
AACCTACACC CACAACCACT GCAGGACTCA CCAAAGCGCT TTGACGTAGA GGTGTCGGTG 
CTGGGCTCTG AAGCCTTCAG TAGCGGTGTC CACTACTGGG AGGTGGTGGT GGCGGAGAAG 
ACCCAGTGGG TGATTGGGCT GGCCCATGAG GCTGCCAGTC GCAAAGGCAG CATCCAGATT 
CAGCCTAGCC GCGGCTTCTA CTGCATCGTT ATGCATGACG GTAACCAGTA CAGCGCCTGC 
ACAGAGCCCT GGACTAGGCT CAACGTCCGT GACAAGCTTG ACAAGGTGGG CGTCTTCCTG 
GACTATGACC AGGGCCTCCT CATCTTCTAC AACGCCGATG ACATGTCCTG GCTCTACACC 
TTCCGAGAGA AGTTTCCTGG CAAGCTCTGC TCCTACTTCA GCCCCGGCCA GAGCCACGCC 
AATGGCAAGA ACGTCCAACC ACTGCGGATC AACACCGTCC GCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835211.1
CDS	31..1458
Translation	MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLANIVERYSAFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFEELQRELKEQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTMDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

Target ORF information:

RefSeq Version	NM_178110.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 62 (Trim62), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178110.2

ORF Insert Sequence:

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGAG AGAACTGAAG 
GAGCAGCTTC AGGCCCTTCA GGACAGCGAG CGGGAACACA CGGAGGCGCT GCAGCTCCTC 
AAGCGACAGT TGGCAGAGAC CAAGTCCTCT ACCAAGAGCC TGCGGACCAC CATCGGGGAA 
GCCTTTGAGC GGCTGCACCG GCTACTTCGA GAGCGACAGA AGGCCATGCT GGAGGAACTG 
GAGGCCGACA CAGCCCGCAC ACTGACGGAC ATTGAGCAGA AGGTCCAGCG TTACAGCCAG 
CAACTACGAA AGGTGCAAGA GGGGGCCCAG ATCCTGCAGG AGCGGCTGGC AGAGACTGAC 
CGCCACACCT TCCTTGCAGG AGTGGCCTCC CTGTCTGAGA GGCTCAAGGG GAAGATTCAT 
GAGACCAACC TAACGTACGA AGACTTCCCG ACCTCCAAGT ACACAGGCCC CCTGCAGTAC 
ACCATCTGGA AATCGCTTTT CCAGGACATC CATCCAGTGC CAGCTGCCCT GACCATGGAC 
CCAGGCACTG CCCACCAGCG CCTGATCCTG TCCGATGACT GCACCATCGT GGCCTACGGG 
AACCTACACC CACAACCACT GCAGGACTCA CCAAAGCGCT TTGACGTAGA GGTGTCGGTG 
CTGGGCTCTG AAGCCTTCAG TAGCGGTGTC CACTACTGGG AGGTGGTGGT GGCGGAGAAG 
ACCCAGTGGG TGATTGGGCT GGCCCATGAG GCTGCCAGTC GCAAAGGCAG CATCCAGATT 
CAGCCTAGCC GCGGCTTCTA CTGCATCGTT ATGCATGACG GTAACCAGTA CAGCGCCTGC 
ACAGAGCCCT GGACTAGGCT CAACGTCCGT GACAAGCTTG ACAAGGTGGG CGTCTTCCTG 
GACTATGACC AGGGCCTCCT CATCTTCTAC AACGCCGATG ACATGTCCTG GCTCTACACC 
TTCCGAGAGA AGTTTCCTGG CAAGCTCTGC TCCTACTTCA GCCCCGGCCA GAGCCACGCC 
AATGGCAAGA ACGTCCAACC ACTGCGGATC AACACCGTCC GCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim62 ( NM_178110.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835211.1 Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 2, mRNA.

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CloneID	OMu07103
Clone ID Related Accession (Same CDS sequence)	NM_178110.2 , NM_178110.3
Accession Version	NM_178110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558886400000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase TRIM62 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL607086.19. On May 28, 2019 this sequence version replaced NM_178110.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC049095.1, SRR7345562.3733633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGAG AGAACTGAAG 
GAGCAGCTTC AGGCCCTTCA GGACAGCGAG CGGGAACACA CGGAGGCGCT GCAGCTCCTC 
AAGCGACAGT TGGCAGAGAC CAAGTCCTCT ACCAAGAGCC TGCGGACCAC CATCGGGGAA 
GCCTTTGAGC GGCTGCACCG GCTACTTCGA GAGCGACAGA AGGCCATGCT GGAGGAACTG 
GAGGCCGACA CAGCCCGCAC ACTGACGGAC ATTGAGCAGA AGGTCCAGCG TTACAGCCAG 
CAACTACGAA AGGTGCAAGA GGGGGCCCAG ATCCTGCAGG AGCGGCTGGC AGAGACTGAC 
CGCCACACCT TCCTTGCAGG AGTGGCCTCC CTGTCTGAGA GGCTCAAGGG GAAGATTCAT 
GAGACCAACC TAACGTACGA AGACTTCCCG ACCTCCAAGT ACACAGGCCC CCTGCAGTAC 
ACCATCTGGA AATCGCTTTT CCAGGACATC CATCCAGTGC CAGCTGCCCT GACCATGGAC 
CCAGGCACTG CCCACCAGCG CCTGATCCTG TCCGATGACT GCACCATCGT GGCCTACGGG 
AACCTACACC CACAACCACT GCAGGACTCA CCAAAGCGCT TTGACGTAGA GGTGTCGGTG 
CTGGGCTCTG AAGCCTTCAG TAGCGGTGTC CACTACTGGG AGGTGGTGGT GGCGGAGAAG 
ACCCAGTGGG TGATTGGGCT GGCCCATGAG GCTGCCAGTC GCAAAGGCAG CATCCAGATT 
CAGCCTAGCC GCGGCTTCTA CTGCATCGTT ATGCATGACG GTAACCAGTA CAGCGCCTGC 
ACAGAGCCCT GGACTAGGCT CAACGTCCGT GACAAGCTTG ACAAGGTGGG CGTCTTCCTG 
GACTATGACC AGGGCCTCCT CATCTTCTAC AACGCCGATG ACATGTCCTG GCTCTACACC 
TTCCGAGAGA AGTTTCCTGG CAAGCTCTGC TCCTACTTCA GCCCCGGCCA GAGCCACGCC 
AATGGCAAGA ACGTCCAACC ACTGCGGATC AACACCGTCC GCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835211.1
CDS	127..1554
Translation	MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLANIVERYSAFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFEELQRELKEQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTMDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

Target ORF information:

RefSeq Version	NM_178110.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 62 (Trim62), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178110.3

ORF Insert Sequence:

ATGGCGTGCA GCCTCAAGGA CGAGCTGCTG TGCTCCATCT GCCTGAGCAT CTACCAGGAC 
CCGGTGAGCC TGGGCTGCGA GCACTACTTC TGCCGCCGCT GCATCACCGA GCACTGGGTG 
CGGCAGGAGG CTCAGGGCGC CCGCGACTGC CCGGAGTGCC GGCGCACGTT CGCCGAGCCC 
GCGCTCGCGC CCAGCCTCAA GCTGGCCAAC ATCGTGGAGC GCTACAGCGC CTTCCCGCTG 
GACGCCATCC TCAACGCGCG CCGAGCCGCG CGACCCTGCC AGGCTCACGA CAAGGTCAAG 
CTCTTCTGCC TCACGGACCG CGCACTGCTC TGCTTCTTCT GTGATGAGCC CGCGCTGCAC 
GAGCAACACC AGGTCACTGG CATCGACGAT GCCTTCGAGG AGTTGCAGAG AGAACTGAAG 
GAGCAGCTTC AGGCCCTTCA GGACAGCGAG CGGGAACACA CGGAGGCGCT GCAGCTCCTC 
AAGCGACAGT TGGCAGAGAC CAAGTCCTCT ACCAAGAGCC TGCGGACCAC CATCGGGGAA 
GCCTTTGAGC GGCTGCACCG GCTACTTCGA GAGCGACAGA AGGCCATGCT GGAGGAACTG 
GAGGCCGACA CAGCCCGCAC ACTGACGGAC ATTGAGCAGA AGGTCCAGCG TTACAGCCAG 
CAACTACGAA AGGTGCAAGA GGGGGCCCAG ATCCTGCAGG AGCGGCTGGC AGAGACTGAC 
CGCCACACCT TCCTTGCAGG AGTGGCCTCC CTGTCTGAGA GGCTCAAGGG GAAGATTCAT 
GAGACCAACC TAACGTACGA AGACTTCCCG ACCTCCAAGT ACACAGGCCC CCTGCAGTAC 
ACCATCTGGA AATCGCTTTT CCAGGACATC CATCCAGTGC CAGCTGCCCT GACCATGGAC 
CCAGGCACTG CCCACCAGCG CCTGATCCTG TCCGATGACT GCACCATCGT GGCCTACGGG 
AACCTACACC CACAACCACT GCAGGACTCA CCAAAGCGCT TTGACGTAGA GGTGTCGGTG 
CTGGGCTCTG AAGCCTTCAG TAGCGGTGTC CACTACTGGG AGGTGGTGGT GGCGGAGAAG 
ACCCAGTGGG TGATTGGGCT GGCCCATGAG GCTGCCAGTC GCAAAGGCAG CATCCAGATT 
CAGCCTAGCC GCGGCTTCTA CTGCATCGTT ATGCATGACG GTAACCAGTA CAGCGCCTGC 
ACAGAGCCCT GGACTAGGCT CAACGTCCGT GACAAGCTTG ACAAGGTGGG CGTCTTCCTG 
GACTATGACC AGGGCCTCCT CATCTTCTAC AACGCCGATG ACATGTCCTG GCTCTACACC 
TTCCGAGAGA AGTTTCCTGG CAAGCTCTGC TCCTACTTCA GCCCCGGCCA GAGCCACGCC 
AATGGCAAGA ACGTCCAACC ACTGCGGATC AACACCGTCC GCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Trim family of genes and the retina: Expression and functional characterization.
PloS one13(9)e0202867(2018)
Chowdhury R,Laboissonniere LA,Wester AK,Muller M,Trimarchi JM

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Ubiquitin Ligase TRIM62 Regulates CARD9-Mediated Anti-fungal Immunity and Intestinal Inflammation.
Immunity43(4)715-26(2015 Oct)
Cao Z,Conway KL,Heath RJ,Rush JS,Leshchiner ES,Ramirez-Ortiz ZG,Nedelsky NB,Huang H,Ng A,Gardet A,Cheng SC,Shamji AF,Rioux JD,Wijmenga C,Netea MG,Means TK,Daly MJ,Xavier RJ

Loss of the novel tumour suppressor and polarity gene Trim62 (Dear1) synergizes with oncogenic Ras in invasive lung cancer.
The Journal of pathology234(1)108-19(2014 Sep)
Quintás-Cardama A,Post SM,Solis LM,Xiong S,Yang P,Chen N,Wistuba II,Killary AM,Lozano G

DEAR1 is a chromosome 1p35 tumor suppressor and master regulator of TGF-β-driven epithelial-mesenchymal transition.
Cancer discovery3(10)1172-89(2013 Oct)
Chen N,Balasenthil S,Reuther J,Frayna A,Wang Y,Chandler DS,Abruzzo LV,Rashid A,Rodriguez J,Lozano G,Cao Y,Lokken E,Chen J,Frazier ML,Sahin AA,Wistuba II,Sen S,Lott ST,Killary AM

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Trim62 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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