Pnpla8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnpla8
Entrez Gene ID	67452
Full Name	patatin-like phospholipase domain containing 8
Synonyms	1200006O19Rik,AI467579,Ipla2(gamma)
General protein information	Preferred Names patatin-like phospholipase domain containing 8 Names calcium-independent phospholipase A2-gamma iPLA2-gamma intracellular membrane-associated calcium-independent phospholipase A2 gamma patatin-like phospholipase domain-containing protein 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 B2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516174.4	XP_006516237.1	calcium-independent phospholipase A2-gamma isoform X1
NM_026164.2	NP_080440.2	calcium-independent phospholipase A2-gamma
XM_006516174.3	XP_006516237.1	calcium-independent phospholipase A2-gamma isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNPLA8	XP_003951157.1
Homo sapiens (human)	PNPLA8	NP_001242936.1
Gallus gallus (chicken)	PNPLA8	NP_001124209.1
Caenorhabditis elegans (roundworm)	Y73B6BL.4	NP_500969.1
Danio rerio (zebrafish)	pnpla8	XP_001918731.2
Bos taurus (cattle)	PNPLA8	XP_005205501.1
Xenopus tropicalis (tropical clawed frog)	pnpla8	XP_002932858.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009956	XP_319089.4
Macaca mulatta (Rhesus monkey)	PNPLA8	XP_001096331.1
Canis lupus familiaris (dog)	PNPLA8	XP_005630992.1
Mus musculus (house mouse)	Pnpla8	NP_080440.2
Rattus norvegicus (Norway rat)	Pnpla8	XP_003750198.1

Gene Ontology
Bibliography

Related articles in PubMed

Omega-3 fatty acid epoxides are autocrine mediators that control the magnitude of IgE-mediated mast cell activation.
Shimanaka Y, Kono N, Taketomi Y, Arita M, Okayama Y, Tanaka Y, Nishito Y, Mochizuki T, Kusuhara H, Adibekian A, Cravatt BF, Murakami M, Arai H
Nature medicine23(11)1287-1297(2017 Nov)

The phospholipase iPLA
Liu GY, Moon SH, Jenkins CM, Li M, Sims HF, Guan S, Gross RW
The Journal of biological chemistry292(25)10672-10684(2017 06)

SREBP-2/PNPLA8 axis improves non-alcoholic fatty liver disease through activation of autophagy.
Kim KY, Jang HJ, Yang YR, Park KI, Seo J, Shin IW, Jeon TI, Ahn SC, Suh PG, Osborne TF, Seo YK
Scientific reports635732(2016 10)

Cardiac Myocyte-specific Knock-out of Calcium-independent Phospholipase A2γ (iPLA2γ) Decreases Oxidized Fatty Acids during Ischemia/Reperfusion and Reduces Infarct Size.
Moon SH, Mancuso DJ, Sims HF, Liu X, Nguyen AL, Yang K, Guan S, Dilthey BG, Jenkins CM, Weinheimer CJ, Kovacs A, Abendschein D, Gross RW
The Journal of biological chemistry291(37)19687-700(2016 09)

Genetic Ablation of Calcium-independent Phospholipase A2γ Induces Glomerular Injury in Mice.
Elimam H, Papillon J, Kaufman DR, Guillemette J, Aoudjit L, Gross RW, Takano T, Cybulsky AV
The Journal of biological chemistry291(28)14468-82(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pnpla8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnpla8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48062	XM_006516174.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu20747	NM_026164.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$489.30 $699.00
View Old Accession Versions >>
OMu48062	XM_006516174.3	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnpla8 ( XM_006516174.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516237.1 Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48062
Clone ID Related Accession (Same CDS sequence)	XM_006516174.4 , XM_006516174.3
Accession Version	XM_006516174.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	calcium-independent phospholipase A2-gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516174.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516237.1
CDS	260..2320
Translation	MSINLTLDIYIYFLNNARSLCGKQRSKQLHFVCSKQYWRMNHVNVHREFHTSKKSCKWNRSEAHCSKHWHSPSNHGLHFGIVRLSTSAPKGLTKVSIHMSRIKSTLNSVSKAIFGSQNEMVTRLAQFKPSSRILRKVSDKGWLKQKNVKQAVESLKNYSDKSAGKNSLAEQKSYFADKEEDSGKHSLFHYTYGITTRFGESFSVLANHINSYFKSKGKMSQTKEDKQLQDKPDLEERKSSSPGPDTVADRPDSESPLEVKDKLSSPTQMPEAHPVSAKQSIANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSPPEEQEVSAKTEQAVNKDKKAEEKKRVLLQQEKIIARVSIDNRTRALVQALRRTADPKLCITRVEELTFHLLEFPEGKGVAIKEKIIPYLLRLRQVKDETLQAAVREILALIGYVDPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSNTWEKILKDRIGSALMIETARNPACPKVAAISTIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGSDLHQDGGLLLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTSTYTSLKTKLSNVISSATDTEG

Target ORF information:

RefSeq Version	XM_006516174.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516174.4

ORF Insert Sequence:

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla8 ( XM_006516174.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516237.1 Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48062
Clone ID Related Accession (Same CDS sequence)	XM_006516174.4 , XM_006516174.3
Accession Version	XM_006516174.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	calcium-independent phospholipase A2-gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516237.1
CDS	262..2322
Translation	MSINLTLDIYIYFLNNARSLCGKQRSKQLHFVCSKQYWRMNHVNVHREFHTSKKSCKWNRSEAHCSKHWHSPSNHGLHFGIVRLSTSAPKGLTKVSIHMSRIKSTLNSVSKAIFGSQNEMVTRLAQFKPSSRILRKVSDKGWLKQKNVKQAVESLKNYSDKSAGKNSLAEQKSYFADKEEDSGKHSLFHYTYGITTRFGESFSVLANHINSYFKSKGKMSQTKEDKQLQDKPDLEERKSSSPGPDTVADRPDSESPLEVKDKLSSPTQMPEAHPVSAKQSIANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSPPEEQEVSAKTEQAVNKDKKAEEKKRVLLQQEKIIARVSIDNRTRALVQALRRTADPKLCITRVEELTFHLLEFPEGKGVAIKEKIIPYLLRLRQVKDETLQAAVREILALIGYVDPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSNTWEKILKDRIGSALMIETARNPACPKVAAISTIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGSDLHQDGGLLLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTSTYTSLKTKLSNVISSATDTEG

Target ORF information:

RefSeq Version	XM_006516174.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516174.3

ORF Insert Sequence:

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla8 ( NM_026164.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080440.2 Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), mRNA.

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CloneID	OMu20747
Clone ID Related Accession (Same CDS sequence)	NM_026164.2
Accession Version	NM_026164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Mus musculus(house mouse)
Product	calcium-independent phospholipase A2-gamma
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ112890.1, AK145776.1, AK033938.1 and AB044139.1. On Nov 17, 2006 this sequence version replaced NM_026164.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB044139.1, SRR1660811.27848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGAAGT TCATATCATG CTTGATGGCC TGTTGCCTTC TGACACGTAC 
TTTAGATTTA ATCCTGTGAT ATGTGAAAAC ATACCCCTAG ATGAAAGCCG GGATGAAAAA 
CTGGACCAGC TTCAGCTGGA AGGGATGAAG TATATAGAAA GAAATGATCA AAAGATGAAA 
AAAGTTGCAA AAATATTAAG TCAAGAAAAA ACAACTCTTC AGAAGATCAA TGATTGGATA 
AAATTAAAAA GTGACATGTA TGAAGGCCTT CCATTTTTTT CAAAATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080440.2
CDS	238..2568
Translation	MSINLTLDIYIYFLNNARSLCGKQRSKQLHFVCSKQYWRMNHVNVHREFHTSKKSCKWNRSEAHCSKHWHSPSNHGLHFGIVRLSTSAPKGLTKVSIHMSRIKSTLNSVSKAIFGSQNEMVTRLAQFKPSSRILRKVSDKGWLKQKNVKQAVESLKNYSDKSAGKNSLAEQKSYFADKEEDSGKHSLFHYTYGITTRFGESFSVLANHINSYFKSKGKMSQTKEDKQLQDKPDLEERKSSSPGPDTVADRPDSESPLEVKDKLSSPTQMPEAHPVSAKQSIANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSPPEEQEVSAKTEQAVNKDKKAEEKKRVLLQQEKIIARVSIDNRTRALVQALRRTADPKLCITRVEELTFHLLEFPEGKGVAIKEKIIPYLLRLRQVKDETLQAAVREILALIGYVDPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSNTWEKILKDRIGSALMIETARNPACPKVAAISTIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGSDLHQDGGLLLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTSTYTSLKTKLSNVISSATDTEEVHIMLDGLLPSDTYFRFNPVICENIPLDESRDEKLDQLQLEGMKYIERNDQKMKKVAKILSQEKTTLQKINDWIKLKSDMYEGLPFFSKL

Target ORF information:

RefSeq Version	NM_026164.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 8 (Pnpla8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026164.2

ORF Insert Sequence:

ATGTCTATTA ATTTGACTCT AGATATATAT ATTTACTTCC TTAATAATGC AAGAAGTCTT 
TGTGGGAAAC AGAGAAGCAA ACAATTGCAT TTTGTGTGTT CTAAACAGTA TTGGCGCATG 
AACCATGTCA ATGTGCACAG AGAGTTTCAC ACAAGTAAGA AAAGCTGTAA ATGGAATAGA 
AGTGAAGCAC ATTGCAGCAA ACACTGGCAC TCTCCAAGCA ACCATGGCTT ACACTTTGGA 
ATTGTGAGAC TTAGCACTTC TGCTCCCAAG GGACTTACAA AAGTGAGCAT TCATATGTCC 
CGTATTAAAA GTACTTTGAA TTCAGTTTCA AAGGCTATTT TTGGCAGTCA AAATGAAATG 
GTTACACGGT TAGCTCAATT TAAGCCAAGT TCTCGAATAT TAAGAAAAGT ATCAGATAAG 
GGATGGTTAA AACAAAAAAA TGTTAAGCAA GCCGTCGAGT CTCTGAAAAA CTATAGTGAT 
AAATCAGCAG GAAAGAATTC TTTAGCAGAA CAGAAAAGTT ACTTTGCAGA TAAAGAAGAA 
GATTCAGGTA AACACAGCCT TTTCCATTAC ACATACGGTA TAACCACCAG ATTTGGAGAG 
TCATTCTCCG TTTTAGCAAA TCATATTAAT TCCTATTTCA AAAGCAAGGG AAAAATGTCT 
CAAACAAAGG AAGATAAACA GCTTCAGGAC AAGCCAGACC TTGAAGAAAG GAAATCCAGT 
TCTCCAGGCC CTGACACCGT GGCAGACAGG CCAGACTCGG AATCTCCCCT AGAAGTTAAA 
GACAAACTTT CCAGCCCGAC CCAGATGCCT GAGGCTCACC CAGTGTCTGC AAAACAAAGT 
ATTGCTAACT TCCTTTCTCG TCCCACTGAA GGCGTACAAG CCTTAGTCGG TGGTTACATT 
GGTGGACTTG TACCCAAATT AAAGTCTGAT CCCAAGAGTC CGCCCGAAGA ACAGGAAGTG 
TCTGCTAAAA CAGAGCAGGC TGTTAACAAA GACAAGAAGG CAGAGGAGAA AAAGCGTGTG 
CTACTTCAGC AAGAAAAGAT TATTGCAAGA GTGAGTATTG ATAACAGAAC TCGAGCCTTA 
GTTCAGGCCT TAAGAAGAAC AGCCGACCCA AAGCTCTGTA TTACTAGGGT TGAAGAACTG 
ACTTTTCATC TTCTAGAATT TCCTGAAGGA AAAGGAGTGG CTATCAAGGA AAAAATTATT 
CCATATTTAT TACGGCTGAG ACAAGTTAAG GATGAAACTC TTCAGGCTGC AGTTAGAGAA 
ATTTTGGCTT TAATTGGTTA TGTAGATCCA GTGAAAGGCA GAGGAATCCG AATCCTCACC 
ATTGATGGTG GAGGAACAAG AGGTGTGGTT GCTCTCCAGA CTCTGAGGAA GTTGGTTGAA 
CTTACTCAGA AGCCGATTCA TCAGCTTTTT GATTACATCT GTGGAGTAAG CACAGGGGCC 
ATATTAGCAT TTATGCTGGG ATTGTTTCAC ATGCCGCTGG ATGAATGTGA GGAACTCTAT 
CGAAAGTTGG GCTCAGATGT GTTCACACAG AATGTCATTG TCGGGACCGT CAAAATGAGT 
TGGAGCCATG CGTTTTATGA CAGTAACACG TGGGAAAAGA TCCTCAAGGA TAGAATTGGA 
TCTGCACTAA TGATTGAAAC AGCGAGAAAC CCCGCATGTC CTAAGGTAGC TGCTATAAGT 
ACCATAGTAA ACAGAGGACA AACACCAAAA GCTTTTGTGT TCAGAAACTA TGGTCATTTT 
CCTGGCACCA ACTCTCACTA TTTAGGAGGC TGTCAGTATA AAATGTGGCA GGCCATTAGA 
GCCTCATCAG CTGCTCCAGG CTACTTTGCA GAGTACGCGC TGGGGAGTGA TCTTCATCAA 
GATGGAGGTT TGCTTCTGAA TAACCCTTCG GCCTTGGCCC TTCACGAATG TAAATGCATC 
TGGCCAGATA CGCCACTGGA GTGTATAGTA TCCTTGGGCA CAGGACGGTA TGAGAGTGAT 
GTGAGGAATA CCTCAACATA CACGAGCCTG AAGACCAAGC TCTCTAATGT TATCAGCAGT 
GCTACAGATA CAGAGGAAGT TCATATCATG CTTGATGGCC TGTTGCCTTC TGACACGTAC 
TTTAGATTTA ATCCTGTGAT ATGTGAAAAC ATACCCCTAG ATGAAAGCCG GGATGAAAAA 
CTGGACCAGC TTCAGCTGGA AGGGATGAAG TATATAGAAA GAAATGATCA AAAGATGAAA 
AAAGTTGCAA AAATATTAAG TCAAGAAAAA ACAACTCTTC AGAAGATCAA TGATTGGATA 
AAATTAAAAA GTGACATGTA TGAAGGCCTT CCATTTTTTT CAAAATTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Omega-3 fatty acid epoxides are autocrine mediators that control the magnitude of IgE-mediated mast cell activation.
Nature medicine23(11)1287-1297(2017 Nov)
Shimanaka Y,Kono N,Taketomi Y,Arita M,Okayama Y,Tanaka Y,Nishito Y,Mochizuki T,Kusuhara H,Adibekian A,Cravatt BF,Murakami M,Arai H

The phospholipase iPLA
The Journal of biological chemistry292(25)10672-10684(2017 06)
Liu GY,Moon SH,Jenkins CM,Li M,Sims HF,Guan S,Gross RW

SREBP-2/PNPLA8 axis improves non-alcoholic fatty liver disease through activation of autophagy.
Scientific reports635732(2016 10)
Kim KY,Jang HJ,Yang YR,Park KI,Seo J,Shin IW,Jeon TI,Ahn SC,Suh PG,Osborne TF,Seo YK

Cardiac Myocyte-specific Knock-out of Calcium-independent Phospholipase A2γ (iPLA2γ) Decreases Oxidized Fatty Acids during Ischemia/Reperfusion and Reduces Infarct Size.
The Journal of biological chemistry291(37)19687-700(2016 09)
Moon SH,Mancuso DJ,Sims HF,Liu X,Nguyen AL,Yang K,Guan S,Dilthey BG,Jenkins CM,Weinheimer CJ,Kovacs A,Abendschein D,Gross RW

Genetic Ablation of Calcium-independent Phospholipase A2γ Induces Glomerular Injury in Mice.
The Journal of biological chemistry291(28)14468-82(2016 Jul)
Elimam H,Papillon J,Kaufman DR,Guillemette J,Aoudjit L,Gross RW,Takano T,Cybulsky AV

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Pnpla8 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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