Cltc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cltc
Entrez Gene ID	67300
Full Name	clathrin, heavy polypeptide (Hc)
Synonyms	3110065L21Rik,CHC,R74732
General protein information	Preferred Names clathrin, heavy polypeptide (Hc) Names clathrin heavy chain 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533983.2	XP_006534046.1	clathrin heavy chain 1 isoform X1
XM_006533984.2	XP_006534047.1	clathrin heavy chain 1 isoform X2
NM_001003908.2	NP_001003908.1	clathrin heavy chain 1 isoform 2
NM_001356393.1	NP_001343322.1	clathrin heavy chain 1 isoform 1
XM_006533985.2	XP_006534048.1	clathrin heavy chain 1 isoform X2
XM_006533983.1	XP_006534046.1	clathrin heavy chain 1 isoform X1
NM_001003908.1	NP_001003908.1	clathrin heavy chain 1
XM_006533985.1	XP_006534048.1	clathrin heavy chain 1 isoform X3

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
Macaca mulatta (Rhesus monkey)	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiomyocyte orientation modulated by the Numb family proteins-N-cadherin axis is essential for ventricular wall morphogenesis.
Miao L, Li J, Li J, Lu Y, Shieh D, Mazurkiewicz JE, Barroso M, Schwarz JJ, Xin HB, Singer HA, Vincent PA, Zhong W, Radice GL, Wan LQ, Fan ZC, Huang G, Wu M
Proceedings of the National Academy of Sciences of the United States of America116(31)15560-15569(2019 Jul)

Clathrin plaques and associated actin anchor intermediate filaments in skeletal muscle.
Franck A, Lainé J, Moulay G, Lemerle E, Trichet M, Gentil C, Benkhelifa-Ziyyat S, Lacène E, Bui MT, Brochier G, Guicheney P, Romero N, Bitoun M, Vassilopoulos S
Molecular biology of the cell30(5)579-590(2019 03)

Redundant and nonredundant organismal functions of EPS15 and EPS15L1.
Milesi C, Alberici P, Pozzi B, Oldani A, Beznoussenko GV, Raimondi A, Soppo BE, Amodio S, Caldieri G, Malabarba MG, Bertalot G, Confalonieri S, Parazzoli D, Mironov AA, Tacchetti C, Di Fiore PP, Sigismund S, Offenhäuser N
Life science alliance2(1)(2019 02)

Regulation of the Natriuretic Peptide Receptor 2 (Npr2) by Phosphorylation of Juxtamembrane Serine and Threonine Residues Is Essential for Bifurcation of Sensory Axons.
Schmidt H, Dickey DM, Dumoulin A, Octave M, Robinson JW, Kühn R, Feil R, Potter LR, Rathjen FG
The Journal of neuroscience : the official journal of the Society for Neuroscience38(45)9768-9780(2018 11)

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.
Honda A, Kita T, Seshadri SV, Misaki K, Ahmed Z, Ladbury JE, Richardson GP, Yonemura S, Ladher RK
Proceedings of the National Academy of Sciences of the United States of America115(33)8388-8393(2018 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cltc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cltc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16667	NM_001003908.2 Latest version!	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu46783	NM_001356393.1 Latest version!	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu46782	XM_006533983.2 Latest version!	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu46783	XM_006533984.2 Latest version!	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu46784	XM_006533985.2 Latest version!	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu46782	XM_006533983.1	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu16667	NM_001003908.1	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu46784	XM_006533985.1	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cltc ( NM_001003908.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003908.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16667
Clone ID Related Accession (Same CDS sequence)	NM_001003908.2 , NM_001003908.1
Accession Version	NM_001003908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570982400000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ103480.1, BB840649.1, BC079897.1, CF745318.1 and BP758029.1. On Oct 4, 2017 this sequence version replaced NM_001003908.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122193.1, BC079897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTG GATAAATTAG ATGCTTCGGA GTCGCTGAGA 
AAAGAAGAAG AACAAGCTAC AGAGACACAG CCCATTGTTT ATGGTCAGCC CCAGCTGATG 
CTAACAGCAG GACCCAGTGT GGCAGTCCCT CCCCAGGCAC CTTTTGGTTA TGGTTATACT 
GCACCACCGT ATGGGCAGCC ACAGCCTGGC TTTGGGTACA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003908.1
CDS	279..5306
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	NM_001003908.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003908.2

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTG GATAAATTAG ATGCTTCGGA GTCGCTGAGA 
AAAGAAGAAG AACAAGCTAC AGAGACACAG CCCATTGTTT ATGGTCAGCC CCAGCTGATG 
CTAACAGCAG GACCCAGTGT GGCAGTCCCT CCCCAGGCAC CTTTTGGTTA TGGTTATACT 
GCACCACCGT ATGGGCAGCC ACAGCCTGGC TTTGGGTACA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( NM_001003908.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003908.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), mRNA.

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CloneID	OMu16667
Clone ID Related Accession (Same CDS sequence)	NM_001003908.2 , NM_001003908.1
Accession Version	NM_001003908.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1493049600000
Organism	Mus musculus(House mouse)
Product	clathrin heavy chain 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079897.1. On Sep 3, 2004 this sequence version replaced XM_181312.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079897.1, AK122193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTG GATAAATTAG ATGCTTCGGA GTCGCTGAGA 
AAAGAAGAAG AACAAGCTAC AGAGACACAG CCCATTGTTT ATGGTCAGCC CCAGCTGATG 
CTAACAGCAG GACCCAGTGT GGCAGTCCCT CCCCAGGCAC CTTTTGGTTA TGGTTATACT 
GCACCACCGT ATGGGCAGCC ACAGCCTGGC TTTGGGTACA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003908.1
CDS	204..5231
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	NM_001003908.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003908.1

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTG GATAAATTAG ATGCTTCGGA GTCGCTGAGA 
AAAGAAGAAG AACAAGCTAC AGAGACACAG CCCATTGTTT ATGGTCAGCC CCAGCTGATG 
CTAACAGCAG GACCCAGTGT GGCAGTCCCT CCCCAGGCAC CTTTTGGTTA TGGTTATACT 
GCACCACCGT ATGGGCAGCC ACAGCCTGGC TTTGGGTACA GCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( NM_001356393.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343322.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 1, mRNA.

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CloneID	OMu46783
Clone ID Related Accession (Same CDS sequence)	XM_006533984.2 , NM_001356393.1
Accession Version	NM_001356393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570982400000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ103480.1, BB840649.1, BC079897.1, AL592222.11, CF745318.1 and BP758029.1. On Oct 4, 2017 this sequence version replaced XM_006533984.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.171830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTT GATGCAATAA AGGAAAAGGT GGATAAATTA 
GATGCTTCGG AGTCGCTGAG AAAAGAAGAA GAACAAGCTA CAGAGACACA GCCCATTGTT 
TATGGTCAGC CCCAGCTGAT GCTAACAGCA GGACCCAGTG TGGCAGTCCC TCCCCAGGCA 
CCTTTTGGTT ATGGTTATAC TGCACCACCG TATGGGCAGC CACAGCCTGG CTTTGGGTAC 
AGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343322.1
CDS	279..5327
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDAIKEKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	NM_001356393.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356393.1

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTT GATGCAATAA AGGAAAAGGT GGATAAATTA 
GATGCTTCGG AGTCGCTGAG AAAAGAAGAA GAACAAGCTA CAGAGACACA GCCCATTGTT 
TATGGTCAGC CCCAGCTGAT GCTAACAGCA GGACCCAGTG TGGCAGTCCC TCCCCAGGCA 
CCTTTTGGTT ATGGTTATAC TGCACCACCG TATGGGCAGC CACAGCCTGG CTTTGGGTAC 
AGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( XM_006533983.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534046.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.

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CloneID	OMu46782
Clone ID Related Accession (Same CDS sequence)	XM_006533983.2 , XM_006533983.1
Accession Version	XM_006533983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTTGATGCAA TAAAGGAAAA GGTGGATAAA 
TTAGATGCTT CGGAGTCGCT GAGAAAAGAA GAAGAACAAG CTACAGAGAC ACAGCCCATT 
GTTTATGGTC AGCCCCAGCT GATGCTAACA GCAGGACCCA GTGTGGCAGT CCCTCCCCAG 
GCACCTTTTG GTTATGGTTA TACTGCACCA CCGTATGGGC AGCCACAGCC TGGCTTTGGG 
TACAGCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534046.1
CDS	175..5226
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKDAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDAIKEKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	XM_006533983.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533983.2

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTTGATGCAA TAAAGGAAAA GGTGGATAAA 
TTAGATGCTT CGGAGTCGCT GAGAAAAGAA GAAGAACAAG CTACAGAGAC ACAGCCCATT 
GTTTATGGTC AGCCCCAGCT GATGCTAACA GCAGGACCCA GTGTGGCAGT CCCTCCCCAG 
GCACCTTTTG GTTATGGTTA TACTGCACCA CCGTATGGGC AGCCACAGCC TGGCTTTGGG 
TACAGCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( XM_006533983.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534046.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.

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CloneID	OMu46782
Clone ID Related Accession (Same CDS sequence)	XM_006533983.2 , XM_006533983.1
Accession Version	XM_006533983.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTTGATGCAA TAAAGGAAAA GGTGGATAAA 
TTAGATGCTT CGGAGTCGCT GAGAAAAGAA GAAGAACAAG CTACAGAGAC ACAGCCCATT 
GTTTATGGTC AGCCCCAGCT GATGCTAACA GCAGGACCCA GTGTGGCAGT CCCTCCCCAG 
GCACCTTTTG GTTATGGTTA TACTGCACCA CCGTATGGGC AGCCACAGCC TGGCTTTGGG 
TACAGCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534046.1
CDS	454..5505
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKDAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDAIKEKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	XM_006533983.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533983.1

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTTGATGCAA TAAAGGAAAA GGTGGATAAA 
TTAGATGCTT CGGAGTCGCT GAGAAAAGAA GAAGAACAAG CTACAGAGAC ACAGCCCATT 
GTTTATGGTC AGCCCCAGCT GATGCTAACA GCAGGACCCA GTGTGGCAGT CCCTCCCCAG 
GCACCTTTTG GTTATGGTTA TACTGCACCA CCGTATGGGC AGCCACAGCC TGGCTTTGGG 
TACAGCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( XM_006533984.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534047.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.

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CloneID	OMu46783
Clone ID Related Accession (Same CDS sequence)	XM_006533984.2 , NM_001356393.1
Accession Version	XM_006533984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	clathrin heavy chain 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTT GATGCAATAA AGGAAAAGGT GGATAAATTA 
GATGCTTCGG AGTCGCTGAG AAAAGAAGAA GAACAAGCTA CAGAGACACA GCCCATTGTT 
TATGGTCAGC CCCAGCTGAT GCTAACAGCA GGACCCAGTG TGGCAGTCCC TCCCCAGGCA 
CCTTTTGGTT ATGGTTATAC TGCACCACCG TATGGGCAGC CACAGCCTGG CTTTGGGTAC 
AGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534047.1
CDS	454..5502
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDAIKEKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	XM_006533984.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533984.2

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG CTGGCAAAAC TCTTCAGATA TTTAACATTG AAATGAAAAG TAAAATGAAG 
GCCCATACCA TGACTGATGA TGTCACCTTC TGGAAATGGA TCTCTTTGAA TACGGTTGCT 
CTTGTTACGG ATAATGCAGT TTATCACTGG AGTATGGAAG GAGAGTCTCA GCCAGTGAAA 
ATGTTTGATC GTCATTCTAG CCTCGCAGGA TGCCAGATCA TCAATTACCG TACAGATGCA 
AAGCAGAAAT GGTTGCTTCT CACTGGCATA TCTGCACAGC AAAATCGTGT GGTTGGAGCT 
ATGCAGCTGT ATTCTGTAGA TAGGAAAGTT TCTCAGCCTA TTGAAGGGCA TGCAGCTAGT 
TTTGCACAAT TTAAGATGGA AGGAAATGCA GAAGAATCAA CATTATTTTG TTTTGCTGTG 
CGGGGTCAGG CAGGAGGAAA GTTACATATC ATTGAAGTTG GTACCCCACC CACAGGGAAT 
CAACCCTTCC CAAAGAAGGC AGTGGATGTT TTCTTTCCTC CAGAAGCACA AAATGACTTT 
CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT TCTTGATTAC CAAGTATGGT 
TATATTCACC TCTATGATCT TGAGACTGGC ACGTGCATCT ACATGAACAG AATCAGCGGA 
GAAACCATCT TCGTCACTGC ACCTCATGAA GCCACAGCTG GAATAATAGG AGTCAACAGA 
AAGGGACAGG TCCTGTCAGT TTGTGTGGAA GAGGAGAACA TAATTCCTTA TATCACCAAT 
GTCCTACAAA ATCCTGACTT GGCTCTGAGA ATGGCTGTGC GGAACAACTT AGCTGGTGCT 
GAAGAACTCT TTGCCCGGAA ATTTAATGCT CTCTTCGCCC AGGGAAACTA CTCAGAGGCT 
GCAAAGGTGG CTGCTAATGC TCCAAAGGGA ATCCTTCGTA CTCCAGATAC CATCCGTCGA 
TTCCAGAGTG TCCCAGCTCA GCCAGGTCAA ACGTCACCTC TACTTCAGTA TTTCGGAATC 
CTTTTGGACC AGGGCCAGCT GAACAAATAC GAGTCCTTAG AGTTATGTAG ACCTGTACTT 
CAGCAAGGGC GGAAACAGCT TTTGGAGAAA TGGCTGAAAG AAGATAAGCT GGAATGTTCT 
GAAGAACTGG GTGATCTCGT TAAATCTGTG GACCCAACAT TAGCACTGAG TGTTTACCTC 
AGGGCCAATG TACCTAATAA AGTCATCCAG TGCTTTGCAG AGACAGGCCA AGTCCAGAAG 
ATTGTTTTAT ATGCTAAAAA AGTTGGCTAT ACTCCAGACT GGATCTTTCT CCTGAGAAAT 
GTCATGCGGA TCAGTCCAGA TCAAGGACAG CAGTTTGCTC AAATGTTAGT TCAGGATGAA 
GAGCCTCTTG CTGATATCAC CCAGATTGTG GATGTCTTTA TGGAATACAA TCTAATCCAA 
CAATGTACTG CCTTCTTACT TGATGCTTTG AAGAATAATC GTCCCTCTGA AGGTCCTTTG 
CAGACGCGAT TGCTTGAGAT GAACCTTATG CACGCACCTC AAGTTGCAGA TGCCATTCTC 
GGGAATCAGA TGTTCACCCA CTATGACCGG GCTCATATCG CCCAGCTGTG TGAGAAGGCG 
GGCCTGCTGC AGCGTGCATT AGAACACTTC ACCGATTTAT ATGACATAAA ACGTGCAGTT 
GTTCATACCC ATCTTCTTAA CCCTGAGTGG TTAGTGAATT ACTTTGGGTC CTTATCAGTT 
GAAGATTCCC TAGAATGCCT CAGAGCCATG CTGTCAGCTA ATATTCGTCA AAATCTACAG 
ATCTGTGTTC AGGTGGCTTC TAAATATCAC GAACAGCTGT CAACTCAGTC TCTGATTGAG 
CTCTTTGAAT CATTCAAGAG TTTTGAAGGT CTCTTTTATT TTCTGGGGTC CATTGTTAAC 
TTTAGTCAGG ACCCAGATGT GCACTTTAAA TATATTCAGG CGGCTTGCAA GACTGGGCAA 
ATCAAAGAAG TGGAAAGAAT CTGCAGAGAA AGCAACTGCT ATGATCCCGA GCGAGTCAAG 
AATTTTCTCA AGGAGGCAAA ACTAACAGAT CAGTTGCCAC TTATCATTGT CTGTGATCGG 
TTTGACTTTG TCCATGACCT GGTGCTGTAT TTATATAGAA ACAATCTTCA GAAGTATATA 
GAGATATATG TCCAGAAGGT AAATCCAAGT CGACTTCCTG TGGTTATTGG AGGATTGCTG 
GATGTTGACT GCTCTGAAGA TGTCATTAAA AATTTGATTC TTGTTGTGAG AGGTCAATTC 
TCTACTGATG AGCTTGTTGC TGAAGTCGAG AAAAGAAACA GATTGAAGCT GCTTCTTCCT 
TGGCTTGAGG CCAGAATTCA TGAGGGCTGT GAGGAGCCTG CTACTCACAA TGCACTTGCC 
AAAATATACA TTGACAGTAA CAACAACCCA GAGAGATTTC TTCGTGAAAA CCCTTATTAT 
GACAGTCGTG TTGTTGGGAA GTACTGTGAG AAGAGAGACC CCCATCTGGC TTGTGTTGCT 
TATGAACGTG GCCAGTGTGA CCTGGAGCTC ATCAATGTTT GCAATGAGAA TTCCCTCTTC 
AAAAGCCTTT CTCGCTACTT AGTCCGTCGA AAGGATCCAG AATTGTGGGG TAGTGTGCTA 
CTGGAAAGCA ATCCATACAG AAGACCGTTA ATTGACCAGG TTGTACAGAC AGCCTTGTCT 
GAAACTCAGG ACCCAGAAGA AGTGTCAGTA ACTGTGAAGG CTTTTATGAC AGCAGATCTT 
CCTAATGAGC TCATTGAACT GCTGGAGAAA ATCGTTCTTG ATAACTCCGT ATTCAGTGAG 
CACAGGAATC TGCAAAATCT CCTCATCCTC ACTGCAATTA AGGCTGACCG AACACGTGTT 
ATGGAGTATA TTAACCGCCT GGATAATTAT GATGCTCCAG ATATTGCCAA CATTGCCATC 
AGCAATGAGC TGTTTGAAGA GGCATTTGCT ATTTTCCGGA AGTTTGATGT CAATACTTCA 
GCAGTGCAGG TGTTGATTGA ACATATTGGA AACCTGGATC GTGCATATGA GTTTGCTGAA 
CGCTGCAATG AGCCTGCTGT TTGGAGTCAA CTTGCAAAAG CTCAGTTGCA GAAAGGAATG 
GTGAAGGAAG CCATTGATTC TTACATCAAA GCAGATGATC CTTCATCTTA CATGGAAGTT 
GTCCAGGCTG CCAATGCCAG CGGAAACTGG GAAGAATTGG TGAAGTATTT GCAGATGGCA 
CGCAAGAAGG CTCGAGAGTC CTATGTGGAG ACAGAATTGA TATTTGCACT GGCTAAAACA 
AACCGCCTTG CAGAATTAGA AGAGTTCATC AATGGGCCAA ATAATGCTCA TATTCAGCAA 
GTTGGTGACC GTTGTTATGA TGAAAAAATG TATGATGCTG CTAAGTTGTT GTACAATAAT 
GTTTCTAACT TTGGACGCTT GGCATCTACC CTTGTTCACC TTGGCGAGTA TCAGGCAGCA 
GTTGATGGTG CCAGAAAAGC TAATAGTACT CGAACATGGA AAGAGGTCTG CTTTGCCTGT 
GTAGATGGGA AAGAGTTCCG CCTTGCTCAG ATGTGTGGAC TCCATATTGT GGTGCATGCA 
GATGAATTAG AGGAGCTTAT CAACTACTAT CAGGATCGTG GATATTTTGA AGAACTCATC 
ACCATGTTGG AGGCAGCCTT GGGACTGGAG CGAGCTCACA TGGGAATGTT CACAGAGCTA 
GCTATTTTGT ATTCTAAATT CAAGCCACAG AAAATGAGAG AACACCTGGA GTTGTTCTGG 
TCGAGAGTGA ATATTCCTAA GGTGCTGAGA GCTGCAGAAC AGGCTCATCT CTGGGCAGAG 
CTGGTGTTTC TGTATGACAA GTACGAAGAG TATGACAATG CCATCATTAC CATGATGAAT 
CACCCCACCG ATGCATGGAA GGAAGGGCAG TTCAAAGACA TCATCACCAA GGTGGCTAAT 
GTGGAACTGT ACTACAAAGC AATCCAGTTC TACTTAGAAT TCAAGCCTTT GTTGTTAAAT 
GACTTGCTCA TGGTGCTGTC TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC 
AAGGTAAAAC AGCTACCACT GGTGAAACCA TATTTACGCT CAGTGCAGAA CCACAACAAC 
AAATCTGTGA ATGAATCACT GAACAACCTC TTTATTACTG AAGAAGATTA CCAGGCTCTG 
CGAACATCAA TAGATGCTTA TGACAACTTT GACAACATCT CACTTGCTCA GCGTTTGGAA 
AAACACGAAC TCATTGAATT CAGAAGAATT GCTGCTTATC TCTTCAAAGG CAACAATCGC 
TGGAAACAGA GTGTAGAGTT GTGCAAGAAA GACAGCCTTT ACAAGGATGC AATGCAGTAT 
GCCTCTGAAT CTAAAGACAC AGAATTGGCT GAAGAGCTCC TACAGTGGTT TTTGCAGGAA 
GAAAAAAGAG AATGCTTTGG AGCTTGTCTC TTTACCTGTT ATGATCTCTT AAGGCCAGAT 
GTCGTCCTGG AAACCGCATG GAGACATAAT ATCATGGATT TCGCCATGCC CTATTTCATC 
CAGGTCATGA AGGAATATTT GACCAAGGTT GATGCAATAA AGGAAAAGGT GGATAAATTA 
GATGCTTCGG AGTCGCTGAG AAAAGAAGAA GAACAAGCTA CAGAGACACA GCCCATTGTT 
TATGGTCAGC CCCAGCTGAT GCTAACAGCA GGACCCAGTG TGGCAGTCCC TCCCCAGGCA 
CCTTTTGGTT ATGGTTATAC TGCACCACCG TATGGGCAGC CACAGCCTGG CTTTGGGTAC 
AGCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( XM_006533985.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534048.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.

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CloneID	OMu46784
Clone ID Related Accession (Same CDS sequence)	XM_006533985.2 , XM_006533985.1
Accession Version	XM_006533985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTGGATAAAT TAGATGCTTC GGAGTCGCTG 
AGAAAAGAAG AAGAACAAGC TACAGAGACA CAGCCCATTG TTTATGGTCA GCCCCAGCTG 
ATGCTAACAG CAGGACCCAG TGTGGCAGTC CCTCCCCAGG CACCTTTTGG TTATGGTTAT 
ACTGCACCAC CGTATGGGCA GCCACAGCCT GGCTTTGGGT ACAGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534048.1
CDS	175..5205
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKDAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	XM_006533985.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533985.2

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTGGATAAAT TAGATGCTTC GGAGTCGCTG 
AGAAAAGAAG AAGAACAAGC TACAGAGACA CAGCCCATTG TTTATGGTCA GCCCCAGCTG 
ATGCTAACAG CAGGACCCAG TGTGGCAGTC CCTCCCCAGG CACCTTTTGG TTATGGTTAT 
ACTGCACCAC CGTATGGGCA GCCACAGCCT GGCTTTGGGT ACAGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cltc ( XM_006533985.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534048.1 Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X3, mRNA.

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CloneID	OMu46784
Clone ID Related Accession (Same CDS sequence)	XM_006533985.2 , XM_006533985.1
Accession Version	XM_006533985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	clathrin heavy chain 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTGGATAAAT TAGATGCTTC GGAGTCGCTG 
AGAAAAGAAG AAGAACAAGC TACAGAGACA CAGCCCATTG TTTATGGTCA GCCCCAGCTG 
ATGCTAACAG CAGGACCCAG TGTGGCAGTC CCTCCCCAGG CACCTTTTGG TTATGGTTAT 
ACTGCACCAC CGTATGGGCA GCCACAGCCT GGCTTTGGGT ACAGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534048.1
CDS	454..5484
Translation	MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKDAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM

Target ORF information:

RefSeq Version	XM_006533985.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus clathrin, heavy polypeptide (Hc) (Cltc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533985.1

ORF Insert Sequence:

ATGGCCCAGA TTCTGCCCAT TCGCTTTCAG GAGCATCTCC AGCTCCAGAA CCTGGGTATC 
AACCCAGCAA ACATTGGCTT CAGTACCTTG ACTATGGAGT CAGACAAATT CATCTGCATT 
AGAGAAAAAG TGGGAGAGCA GGCCCAGGTG GTAATCATTG ATATGAATGA TCCAAGTAAT 
CCAATTCGAA GACCAATCTC AGCAGACAGT GCCATCATGA ATCCTGCCAG CAAAGTGATT 
GCACTGAAAG ATGCTGGCAA AACTCTTCAG ATATTTAACA TTGAAATGAA AAGTAAAATG 
AAGGCCCATA CCATGACTGA TGATGTCACC TTCTGGAAAT GGATCTCTTT GAATACGGTT 
GCTCTTGTTA CGGATAATGC AGTTTATCAC TGGAGTATGG AAGGAGAGTC TCAGCCAGTG 
AAAATGTTTG ATCGTCATTC TAGCCTCGCA GGATGCCAGA TCATCAATTA CCGTACAGAT 
GCAAAGCAGA AATGGTTGCT TCTCACTGGC ATATCTGCAC AGCAAAATCG TGTGGTTGGA 
GCTATGCAGC TGTATTCTGT AGATAGGAAA GTTTCTCAGC CTATTGAAGG GCATGCAGCT 
AGTTTTGCAC AATTTAAGAT GGAAGGAAAT GCAGAAGAAT CAACATTATT TTGTTTTGCT 
GTGCGGGGTC AGGCAGGAGG AAAGTTACAT ATCATTGAAG TTGGTACCCC ACCCACAGGG 
AATCAACCCT TCCCAAAGAA GGCAGTGGAT GTTTTCTTTC CTCCAGAAGC ACAAAATGAC 
TTTCCTGTTG CAATGCAGAT CAGTGAAAAG CATGATGTGG TGTTCTTGAT TACCAAGTAT 
GGTTATATTC ACCTCTATGA TCTTGAGACT GGCACGTGCA TCTACATGAA CAGAATCAGC 
GGAGAAACCA TCTTCGTCAC TGCACCTCAT GAAGCCACAG CTGGAATAAT AGGAGTCAAC 
AGAAAGGGAC AGGTCCTGTC AGTTTGTGTG GAAGAGGAGA ACATAATTCC TTATATCACC 
AATGTCCTAC AAAATCCTGA CTTGGCTCTG AGAATGGCTG TGCGGAACAA CTTAGCTGGT 
GCTGAAGAAC TCTTTGCCCG GAAATTTAAT GCTCTCTTCG CCCAGGGAAA CTACTCAGAG 
GCTGCAAAGG TGGCTGCTAA TGCTCCAAAG GGAATCCTTC GTACTCCAGA TACCATCCGT 
CGATTCCAGA GTGTCCCAGC TCAGCCAGGT CAAACGTCAC CTCTACTTCA GTATTTCGGA 
ATCCTTTTGG ACCAGGGCCA GCTGAACAAA TACGAGTCCT TAGAGTTATG TAGACCTGTA 
CTTCAGCAAG GGCGGAAACA GCTTTTGGAG AAATGGCTGA AAGAAGATAA GCTGGAATGT 
TCTGAAGAAC TGGGTGATCT CGTTAAATCT GTGGACCCAA CATTAGCACT GAGTGTTTAC 
CTCAGGGCCA ATGTACCTAA TAAAGTCATC CAGTGCTTTG CAGAGACAGG CCAAGTCCAG 
AAGATTGTTT TATATGCTAA AAAAGTTGGC TATACTCCAG ACTGGATCTT TCTCCTGAGA 
AATGTCATGC GGATCAGTCC AGATCAAGGA CAGCAGTTTG CTCAAATGTT AGTTCAGGAT 
GAAGAGCCTC TTGCTGATAT CACCCAGATT GTGGATGTCT TTATGGAATA CAATCTAATC 
CAACAATGTA CTGCCTTCTT ACTTGATGCT TTGAAGAATA ATCGTCCCTC TGAAGGTCCT 
TTGCAGACGC GATTGCTTGA GATGAACCTT ATGCACGCAC CTCAAGTTGC AGATGCCATT 
CTCGGGAATC AGATGTTCAC CCACTATGAC CGGGCTCATA TCGCCCAGCT GTGTGAGAAG 
GCGGGCCTGC TGCAGCGTGC ATTAGAACAC TTCACCGATT TATATGACAT AAAACGTGCA 
GTTGTTCATA CCCATCTTCT TAACCCTGAG TGGTTAGTGA ATTACTTTGG GTCCTTATCA 
GTTGAAGATT CCCTAGAATG CCTCAGAGCC ATGCTGTCAG CTAATATTCG TCAAAATCTA 
CAGATCTGTG TTCAGGTGGC TTCTAAATAT CACGAACAGC TGTCAACTCA GTCTCTGATT 
GAGCTCTTTG AATCATTCAA GAGTTTTGAA GGTCTCTTTT ATTTTCTGGG GTCCATTGTT 
AACTTTAGTC AGGACCCAGA TGTGCACTTT AAATATATTC AGGCGGCTTG CAAGACTGGG 
CAAATCAAAG AAGTGGAAAG AATCTGCAGA GAAAGCAACT GCTATGATCC CGAGCGAGTC 
AAGAATTTTC TCAAGGAGGC AAAACTAACA GATCAGTTGC CACTTATCAT TGTCTGTGAT 
CGGTTTGACT TTGTCCATGA CCTGGTGCTG TATTTATATA GAAACAATCT TCAGAAGTAT 
ATAGAGATAT ATGTCCAGAA GGTAAATCCA AGTCGACTTC CTGTGGTTAT TGGAGGATTG 
CTGGATGTTG ACTGCTCTGA AGATGTCATT AAAAATTTGA TTCTTGTTGT GAGAGGTCAA 
TTCTCTACTG ATGAGCTTGT TGCTGAAGTC GAGAAAAGAA ACAGATTGAA GCTGCTTCTT 
CCTTGGCTTG AGGCCAGAAT TCATGAGGGC TGTGAGGAGC CTGCTACTCA CAATGCACTT 
GCCAAAATAT ACATTGACAG TAACAACAAC CCAGAGAGAT TTCTTCGTGA AAACCCTTAT 
TATGACAGTC GTGTTGTTGG GAAGTACTGT GAGAAGAGAG ACCCCCATCT GGCTTGTGTT 
GCTTATGAAC GTGGCCAGTG TGACCTGGAG CTCATCAATG TTTGCAATGA GAATTCCCTC 
TTCAAAAGCC TTTCTCGCTA CTTAGTCCGT CGAAAGGATC CAGAATTGTG GGGTAGTGTG 
CTACTGGAAA GCAATCCATA CAGAAGACCG TTAATTGACC AGGTTGTACA GACAGCCTTG 
TCTGAAACTC AGGACCCAGA AGAAGTGTCA GTAACTGTGA AGGCTTTTAT GACAGCAGAT 
CTTCCTAATG AGCTCATTGA ACTGCTGGAG AAAATCGTTC TTGATAACTC CGTATTCAGT 
GAGCACAGGA ATCTGCAAAA TCTCCTCATC CTCACTGCAA TTAAGGCTGA CCGAACACGT 
GTTATGGAGT ATATTAACCG CCTGGATAAT TATGATGCTC CAGATATTGC CAACATTGCC 
ATCAGCAATG AGCTGTTTGA AGAGGCATTT GCTATTTTCC GGAAGTTTGA TGTCAATACT 
TCAGCAGTGC AGGTGTTGAT TGAACATATT GGAAACCTGG ATCGTGCATA TGAGTTTGCT 
GAACGCTGCA ATGAGCCTGC TGTTTGGAGT CAACTTGCAA AAGCTCAGTT GCAGAAAGGA 
ATGGTGAAGG AAGCCATTGA TTCTTACATC AAAGCAGATG ATCCTTCATC TTACATGGAA 
GTTGTCCAGG CTGCCAATGC CAGCGGAAAC TGGGAAGAAT TGGTGAAGTA TTTGCAGATG 
GCACGCAAGA AGGCTCGAGA GTCCTATGTG GAGACAGAAT TGATATTTGC ACTGGCTAAA 
ACAAACCGCC TTGCAGAATT AGAAGAGTTC ATCAATGGGC CAAATAATGC TCATATTCAG 
CAAGTTGGTG ACCGTTGTTA TGATGAAAAA ATGTATGATG CTGCTAAGTT GTTGTACAAT 
AATGTTTCTA ACTTTGGACG CTTGGCATCT ACCCTTGTTC ACCTTGGCGA GTATCAGGCA 
GCAGTTGATG GTGCCAGAAA AGCTAATAGT ACTCGAACAT GGAAAGAGGT CTGCTTTGCC 
TGTGTAGATG GGAAAGAGTT CCGCCTTGCT CAGATGTGTG GACTCCATAT TGTGGTGCAT 
GCAGATGAAT TAGAGGAGCT TATCAACTAC TATCAGGATC GTGGATATTT TGAAGAACTC 
ATCACCATGT TGGAGGCAGC CTTGGGACTG GAGCGAGCTC ACATGGGAAT GTTCACAGAG 
CTAGCTATTT TGTATTCTAA ATTCAAGCCA CAGAAAATGA GAGAACACCT GGAGTTGTTC 
TGGTCGAGAG TGAATATTCC TAAGGTGCTG AGAGCTGCAG AACAGGCTCA TCTCTGGGCA 
GAGCTGGTGT TTCTGTATGA CAAGTACGAA GAGTATGACA ATGCCATCAT TACCATGATG 
AATCACCCCA CCGATGCATG GAAGGAAGGG CAGTTCAAAG ACATCATCAC CAAGGTGGCT 
AATGTGGAAC TGTACTACAA AGCAATCCAG TTCTACTTAG AATTCAAGCC TTTGTTGTTA 
AATGACTTGC TCATGGTGCT GTCTCCACGG TTGGATCACA CTCGTGCAGT CAATTATTTC 
AGCAAGGTAA AACAGCTACC ACTGGTGAAA CCATATTTAC GCTCAGTGCA GAACCACAAC 
AACAAATCTG TGAATGAATC ACTGAACAAC CTCTTTATTA CTGAAGAAGA TTACCAGGCT 
CTGCGAACAT CAATAGATGC TTATGACAAC TTTGACAACA TCTCACTTGC TCAGCGTTTG 
GAAAAACACG AACTCATTGA ATTCAGAAGA ATTGCTGCTT ATCTCTTCAA AGGCAACAAT 
CGCTGGAAAC AGAGTGTAGA GTTGTGCAAG AAAGACAGCC TTTACAAGGA TGCAATGCAG 
TATGCCTCTG AATCTAAAGA CACAGAATTG GCTGAAGAGC TCCTACAGTG GTTTTTGCAG 
GAAGAAAAAA GAGAATGCTT TGGAGCTTGT CTCTTTACCT GTTATGATCT CTTAAGGCCA 
GATGTCGTCC TGGAAACCGC ATGGAGACAT AATATCATGG ATTTCGCCAT GCCCTATTTC 
ATCCAGGTCA TGAAGGAATA TTTGACCAAG GTGGATAAAT TAGATGCTTC GGAGTCGCTG 
AGAAAAGAAG AAGAACAAGC TACAGAGACA CAGCCCATTG TTTATGGTCA GCCCCAGCTG 
ATGCTAACAG CAGGACCCAG TGTGGCAGTC CCTCCCCAGG CACCTTTTGG TTATGGTTAT 
ACTGCACCAC CGTATGGGCA GCCACAGCCT GGCTTTGGGT ACAGCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiomyocyte orientation modulated by the Numb family proteins-N-cadherin axis is essential for ventricular wall morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America116(31)15560-15569(2019 Jul)
Miao L,Li J,Li J,Lu Y,Shieh D,Mazurkiewicz JE,Barroso M,Schwarz JJ,Xin HB,Singer HA,Vincent PA,Zhong W,Radice GL,Wan LQ,Fan ZC,Huang G,Wu M

Clathrin plaques and associated actin anchor intermediate filaments in skeletal muscle.
Molecular biology of the cell30(5)579-590(2019 03)
Franck A,Lainé J,Moulay G,Lemerle E,Trichet M,Gentil C,Benkhelifa-Ziyyat S,Lacène E,Bui MT,Brochier G,Guicheney P,Romero N,Bitoun M,Vassilopoulos S

Redundant and nonredundant organismal functions of EPS15 and EPS15L1.
Life science alliance2(1)(2019 02)
Milesi C,Alberici P,Pozzi B,Oldani A,Beznoussenko GV,Raimondi A,Soppo BE,Amodio S,Caldieri G,Malabarba MG,Bertalot G,Confalonieri S,Parazzoli D,Mironov AA,Tacchetti C,Di Fiore PP,Sigismund S,Offenhäuser N

Regulation of the Natriuretic Peptide Receptor 2 (Npr2) by Phosphorylation of Juxtamembrane Serine and Threonine Residues Is Essential for Bifurcation of Sensory Axons.
The Journal of neuroscience : the official journal of the Society for Neuroscience38(45)9768-9780(2018 11)
Schmidt H,Dickey DM,Dumoulin A,Octave M,Robinson JW,Kühn R,Feil R,Potter LR,Rathjen FG

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.
Proceedings of the National Academy of Sciences of the United States of America115(33)8388-8393(2018 08)
Honda A,Kita T,Seshadri SV,Misaki K,Ahmed Z,Ladbury JE,Richardson GP,Yonemura S,Ladher RK

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Cltc cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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