Cdk5rap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk5rap1
Entrez Gene ID	66971
Full Name	CDK5 regulatory subunit associated protein 1
Synonyms	2310066P17Rik
General protein information	Preferred Names CDK5 regulatory subunit associated protein 1 Names CDK5 regulatory subunit-associated protein 1 CDK5 activator-binding protein C42
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025876.2	NP_080152.1	CDK5 regulatory subunit-associated protein 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CDK5RAP1	XP_417464.2
Homo sapiens (human)	CDK5RAP1	NP_057492.2
Pan troglodytes (chimpanzee)	CDK5RAP1	XP_001155512.2
Bos taurus (cattle)	CDK5RAP1	NP_001069114.1
Oryza sativa (rice)	Os11g0592800	NP_001068196.1
Mus musculus (house mouse)	Cdk5rap1	NP_080152.1
Caenorhabditis elegans (roundworm)	F25B5.5	NP_498290.1
Xenopus tropicalis (tropical clawed frog)	cdk5rap1	NP_001017192.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012104	XP_320425.3
Rattus norvegicus (Norway rat)	Cdk5rap1	NP_663773.1
Danio rerio (zebrafish)	zgc:162738	NP_001103849.1
Drosophila melanogaster (fruit fly)	CG6345	NP_650002.1
Arabidopsis thaliana (thale cress)	AT4G36390	NP_195357.2
Canis lupus familiaris (dog)	CDK5RAP1	XP_005634997.1

Gene Ontology
Bibliography

Related articles in PubMed

Neddylation mediates ventricular chamber maturation through repression of Hippo signaling.
Zou J, Ma W, Li J, Littlejohn R, Zhou H, Kim IM, Fulton DJR, Chen W, Weintraub NL, Zhou J, Su H
Proceedings of the National Academy of Sciences of the United States of America115(17)E4101-E4110(2018 04)

Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.
Wei FY, Zhou B, Suzuki T, Miyata K, Ujihara Y, Horiguchi H, Takahashi N, Xie P, Michiue H, Fujimura A, Kaitsuka T, Matsui H, Koga Y, Mohri S, Suzuki T, Oike Y, Tomizawa K
Cell metabolism21(3)428-42(2015 Mar)

Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.
Koss M, Bolze A, Brendolan A, Saggese M, Capellini TD, Bojilova E, Boisson B, Prall OW, Elliott DA, Solloway M, Lenti E, Hidaka C, Chang CP, Mahlaoui N, Harvey RP, Casanova JL, Selleri L
Developmental cell22(5)913-26(2012 May)

Mutagenesis of diploid mammalian genes by gene entrapment.
Lin Q, Donahue SL, Moore-Jarrett T, Cao S, Osipovich AB, Ruley HE
Nucleic acids research34(20)e139(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cdk5rap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk5rap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13546	NM_025876.2 Latest version!	Mus musculus CDK5 regulatory subunit associated protein 1 (Cdk5rap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk5rap1 ( NM_025876.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080152.1 Mus musculus CDK5 regulatory subunit associated protein 1 (Cdk5rap1), mRNA.

Price & Availability↑

CloneID	OMu13546
Clone ID Related Accession (Same CDS sequence)	NM_025876.2
Accession Version	NM_025876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	CDK5 regulatory subunit-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL928894.12 and AL732601.16. On Nov 17, 2006 this sequence version replaced NM_025876.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK010081.1, AK088517.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCATCCTT TACGGTGTGT CCTCCAAGTA CAGAGGTTGT CAGCACCATT CACCTCCATG 
TGCTGGGTGT TGCTTAGGAC CTGCAGAGCA CAAAGCAGTG TGTCCAGCAC TCCTTGTCCC 
AGTCCAGAGG CGAAGAGCTC AGAAGCTCAG AAGGACTTCA GCTCCAGGCT AGCCACTGGA 
CCGACTTTTC AGCATTTTTT AAGAAGTGCT TCAGTTCCTC AAGAGAAACC ATCTTCTCCA 
GAGGTGGAGG ACCCACCTCC CTATCTCTCG GGGGATGAAC TTCTAGGAAG GCAGAGAAAA 
GTCTACCTCG AGACCTATGG CTGTCAGATG AACGTGAACG ACACAGAGAT AGCCTGGTCC 
ATCTTACAGA AGAGTGGCTA CCTTCGGACC AGCAACCTCC AAGAGGCTGA TGTGATCCTT 
CTTGTCACGT GTTCTATCAG GGAGAAGGCC GAGCAGACCA TCTGGAACCG CTTACATCAG 
CTCAAAGTCC TGAAGACAAA GCGGCCACGC TCTCGAGTAC CTCTGAGGAT TGGGATTCTA 
GGCTGCATGG CTGAGAGGCT GAAGGGAGAG ATCCTCAACA GGGAGAAGAT GGTAGATCTC 
TTGGCTGGTC CAGACGCCTA CCGAGACCTT CCCCGGCTGC TGGCTGTGGT GGAGTCAGGT 
CAGCAAGCAG CAAATGTGCT TCTCTCTCTG GATGAGACCT ACGCAGATAT CATGCCAGTC 
CAGACGAGCC CCAGTGCCAC TTCTGCCTTT GTATCCATTA TGCGGGGCTG CGACAACATG 
TGCAGTTACT GTATCGTGCC GTTCACGCGT GGCCGGGAGA GGAGCCGGCC AGTTGCCTCC 
ATTCTCGATG AAGTGAGGAA GCTGTCCGAG CAGGGCCTAA AAGAAGTGAC ACTTCTTGGT 
CAGAATGTTA ATAGTTTTCG GGACAATTCG GAAGTCCAGT TCAATAATGC AGGGTCTGCT 
AACCTCAGCC GTGGCTTTAC CACCAACTAT AAACCCAAAC AAGGAGGGCT TCGTTTTTCT 
CACCTTCTGG ATCAGGTTTC AAGAATCGAT CCTGAAATGA GGATCCGATT CACCTCTCCT 
CACCCCAAGG ATTTTCCTGA TGAGGTTCTT CAGCTGATTC GTGAGAGACA CAACATCTGT 
AAGCAGATCC ACCTGCCAGC CCAGAGTGGA AGCAGCCGTG TACTGGATGC CATGCGGAGA 
GGATATTCAA GAGAAGCATA TGTGGCATTA GTTCATCATG TCAGAGAGAC TATCCCAGGT 
GTGAGCCTTA GCAGCGACTT CATCACTGGC TTTTGTGGAG AGACAGAGGA TGATCACCGG 
CAGACAGTGT CTTTACTTAG AGAAGTGCAG TATAATACCG GTTTTCTCTT TGCATACAGC 
ATGAGGCAAA AGACACGAGC ATATCACAGG CTGAAGGATG ATGTTCCAGA AGAAGTAAAA 
TTAAGGCGTT TGGAGGAACT CATTACTGTC TTCAGAGAAG AAGCTTCAAA AGCCAATAAG 
ACCTCAGTGG GTTGTAGCCA GCTGGTACTG GTTGAAGGGT TCAGCAAACG TTCTACCACA 
GACCTTTGTG GCCGGAATGA TGCAAACCTT AAGGTGATTT TCCCTGACGC AGAGGTGGAG 
GATATCACTA ACCCTGGGCT CAAGGTCAGA GCTCAACCTG GGGACTATGT GCTGGTGAAG 
ATCACCTCTG CCAGCTCTCA GACCCTGAAA GGACACATTC TCTGCAGGAC CACTATGAAA 
GACTCATTGA CGTATTGCAC GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080152.1
CDS	59..1825
Translation	MHPLRCVLQVQRLSAPFTSMCWVLLRTCRAQSSVSSTPCPSPEAKSSEAQKDFSSRLATGPTFQHFLRSASVPQEKPSSPEVEDPPPYLSGDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDLPRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITNPGLKVRAQPGDYVLVKITSASSQTLKGHILCRTTMKDSLTYCTT

Target ORF information:

RefSeq Version	NM_025876.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDK5 regulatory subunit associated protein 1 (Cdk5rap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025876.2

ORF Insert Sequence:

ATGCATCCTT TACGGTGTGT CCTCCAAGTA CAGAGGTTGT CAGCACCATT CACCTCCATG 
TGCTGGGTGT TGCTTAGGAC CTGCAGAGCA CAAAGCAGTG TGTCCAGCAC TCCTTGTCCC 
AGTCCAGAGG CGAAGAGCTC AGAAGCTCAG AAGGACTTCA GCTCCAGGCT AGCCACTGGA 
CCGACTTTTC AGCATTTTTT AAGAAGTGCT TCAGTTCCTC AAGAGAAACC ATCTTCTCCA 
GAGGTGGAGG ACCCACCTCC CTATCTCTCG GGGGATGAAC TTCTAGGAAG GCAGAGAAAA 
GTCTACCTCG AGACCTATGG CTGTCAGATG AACGTGAACG ACACAGAGAT AGCCTGGTCC 
ATCTTACAGA AGAGTGGCTA CCTTCGGACC AGCAACCTCC AAGAGGCTGA TGTGATCCTT 
CTTGTCACGT GTTCTATCAG GGAGAAGGCC GAGCAGACCA TCTGGAACCG CTTACATCAG 
CTCAAAGTCC TGAAGACAAA GCGGCCACGC TCTCGAGTAC CTCTGAGGAT TGGGATTCTA 
GGCTGCATGG CTGAGAGGCT GAAGGGAGAG ATCCTCAACA GGGAGAAGAT GGTAGATCTC 
TTGGCTGGTC CAGACGCCTA CCGAGACCTT CCCCGGCTGC TGGCTGTGGT GGAGTCAGGT 
CAGCAAGCAG CAAATGTGCT TCTCTCTCTG GATGAGACCT ACGCAGATAT CATGCCAGTC 
CAGACGAGCC CCAGTGCCAC TTCTGCCTTT GTATCCATTA TGCGGGGCTG CGACAACATG 
TGCAGTTACT GTATCGTGCC GTTCACGCGT GGCCGGGAGA GGAGCCGGCC AGTTGCCTCC 
ATTCTCGATG AAGTGAGGAA GCTGTCCGAG CAGGGCCTAA AAGAAGTGAC ACTTCTTGGT 
CAGAATGTTA ATAGTTTTCG GGACAATTCG GAAGTCCAGT TCAATAATGC AGGGTCTGCT 
AACCTCAGCC GTGGCTTTAC CACCAACTAT AAACCCAAAC AAGGAGGGCT TCGTTTTTCT 
CACCTTCTGG ATCAGGTTTC AAGAATCGAT CCTGAAATGA GGATCCGATT CACCTCTCCT 
CACCCCAAGG ATTTTCCTGA TGAGGTTCTT CAGCTGATTC GTGAGAGACA CAACATCTGT 
AAGCAGATCC ACCTGCCAGC CCAGAGTGGA AGCAGCCGTG TACTGGATGC CATGCGGAGA 
GGATATTCAA GAGAAGCATA TGTGGCATTA GTTCATCATG TCAGAGAGAC TATCCCAGGT 
GTGAGCCTTA GCAGCGACTT CATCACTGGC TTTTGTGGAG AGACAGAGGA TGATCACCGG 
CAGACAGTGT CTTTACTTAG AGAAGTGCAG TATAATACCG GTTTTCTCTT TGCATACAGC 
ATGAGGCAAA AGACACGAGC ATATCACAGG CTGAAGGATG ATGTTCCAGA AGAAGTAAAA 
TTAAGGCGTT TGGAGGAACT CATTACTGTC TTCAGAGAAG AAGCTTCAAA AGCCAATAAG 
ACCTCAGTGG GTTGTAGCCA GCTGGTACTG GTTGAAGGGT TCAGCAAACG TTCTACCACA 
GACCTTTGTG GCCGGAATGA TGCAAACCTT AAGGTGATTT TCCCTGACGC AGAGGTGGAG 
GATATCACTA ACCCTGGGCT CAAGGTCAGA GCTCAACCTG GGGACTATGT GCTGGTGAAG 
ATCACCTCTG CCAGCTCTCA GACCCTGAAA GGACACATTC TCTGCAGGAC CACTATGAAA 
GACTCATTGA CGTATTGCAC GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neddylation mediates ventricular chamber maturation through repression of Hippo signaling.
Proceedings of the National Academy of Sciences of the United States of America115(17)E4101-E4110(2018 04)
Zou J,Ma W,Li J,Littlejohn R,Zhou H,Kim IM,Fulton DJR,Chen W,Weintraub NL,Zhou J,Su H

Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.
Cell metabolism21(3)428-42(2015 Mar)
Wei FY,Zhou B,Suzuki T,Miyata K,Ujihara Y,Horiguchi H,Takahashi N,Xie P,Michiue H,Fujimura A,Kaitsuka T,Matsui H,Koga Y,Mohri S,Suzuki T,Oike Y,Tomizawa K

Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.
Developmental cell22(5)913-26(2012 May)
Koss M,Bolze A,Brendolan A,Saggese M,Capellini TD,Bojilova E,Boisson B,Prall OW,Elliott DA,Solloway M,Lenti E,Hidaka C,Chang CP,Mahlaoui N,Harvey RP,Casanova JL,Selleri L

Mutagenesis of diploid mammalian genes by gene entrapment.
Nucleic acids research34(20)e139(2006)
Lin Q,Donahue SL,Moore-Jarrett T,Cao S,Osipovich AB,Ruley HE

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Cdk5rap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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