Henmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Henmt1
Entrez Gene ID	66715
Full Name	HEN1 methyltransferase homolog 1 (Arabidopsis)
Synonyms	4921515J06Rik,Hen1,mHEN1
General protein information	Preferred Names HEN1 methyltransferase homolog 1 (Arabidopsis) Names small RNA 2'-O-methyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501898.2	XP_006501961.1	small RNA 2'-O-methyltransferase isoform X1
NM_001078646.1	NP_001072114.1	small RNA 2'-O-methyltransferase
XM_006501898.3	XP_006501961.1	small RNA 2'-O-methyltransferase isoform X1
NM_025723.2	NP_079999.2	small RNA 2'-O-methyltransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC479920	XP_537046.2
Xenopus tropicalis (tropical clawed frog)	henmt1	NP_001153012.1
Macaca mulatta (Rhesus monkey)	HENMT1	XP_001087383.2
Mus musculus (house mouse)	Henmt1	NP_001072114.1
Gallus gallus (chicken)	HENMT1	XP_426611.1
Danio rerio (zebrafish)	henmt1	NP_001017842.1
Drosophila melanogaster (fruit fly)	Hen1	NP_610732.1
Homo sapiens (human)	HENMT1	NP_001096062.1
Pan troglodytes (chimpanzee)	HENMT1	XP_001144373.1
Bos taurus (cattle)	HENMT1	XP_005204292.1
Rattus norvegicus (Norway rat)	Henmt1	NP_001032744.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
Peng L, Zhang F, Shang R, Wang X, Chen J, Chou JJ, Ma J, Wu L, Huang Y
The Journal of biological chemistry293(26)9981-9994(2018 06)

HENMT1 and piRNA Stability Are Required for Adult Male Germ Cell Transposon Repression and to Define the Spermatogenic Program in the Mouse.
Lim SL, Qu ZP, Kortschak RD, Lawrence DM, Geoghegan J, Hempfling AL, Bergmann M, Goodnow CC, Ormandy CJ, Wong L, Mann J, Scott HS, Jamsai D, Adelson DL, O'Bryan MK
PLoS genetics11(10)e1005620(2015 Oct)

The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs.
Kirino Y, Mourelatos Z
RNA (New York, N.Y.)13(9)1397-401(2007 Sep)

2'-O-methyl modification in mouse piRNAs and its methylase.
Kirino Y, Mourelatos Z
Nucleic acids symposium series (2004)(51)417-8(2007)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Henmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Henmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00935	NM_025723.2 Latest version!	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu00935	NM_001078646.1 Latest version!	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu32177	XM_006501898.3 Latest version!	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu32177	XM_006501898.2	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Henmt1 ( NM_001078646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001072114.1 Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00935
Clone ID Related Accession (Same CDS sequence)	NM_001078646.1 , NM_025723.2
Accession Version	NM_001078646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	small RNA 2'-O-methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK038994.1 and BC099408.1. ##Evidence-Data-START## Transcript exon combination :: BC099408.1, AK038994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGG CAGAAAGCAT ACCGTGCAAT AGTGTGGTTG GTGGCAATTT TAAAGAAGTT 
AGTCCAGAGA AGGTAATTCG GTTCAAACCA CCATTATACA AACAACGGTA TCAGTTTGTT 
AGAGATTTAG TGGATCGCCA TGAACCCAAG AAGGTTGCCG ACCTGGGATG TGGAGATGCC 
AAGCTCCTAA AGCTGCTAAA AATCTACCCA TGCATTCAAC TGCTTGTTGG GGTGGATATC 
AATGAAGAAA AATTGCATTC AAATGGGCAT CGCTTGTCTC CCTATTTGGG GGAGTTTGTA 
AAACCCCGAG ATCTAGATTT GACTGTCACC TTGTATCATG GCTCTGTTGT GGAGAGAGAC 
TCCCGCTTGC TTGGATTCGA TTTGATAACA TGCATTGAAT TAATAGAACA CTTGGATTCA 
GATGATCTGG CCAGATTTCC TGATGTGGTT TTTGGATACC TGTCACCAGC CATGGTTGTC 
ATCAGCACCC CAAATGCTGA ATTCAACCCC CTGTTTCCTA CAGTCACCCT GAGGGACGCC 
GACCATAAAT TTGAGTGGAG CAGAATGGAG TTTCAGACCT GGGCTTTACA CGTGGCAAAC 
TGCTACAATT ACCGTGTGGA GTTTACTGGG GTAGGGACAC CGCCAGCTGG ATCTGAGCAT 
GTTGGATATT GTACCCAGAT AGGTGTCTTT ACAAAGAATG GCGGGAAGCT CTCTAAACCT 
TCGGTTTCCC AGCAGTGTGA CCAGCACGTT TATAAACCTG TTTATACAAC CTCCTACCCG 
AGTTTACAGC AGGAGAAGGT GCTCAAGTTT GTTCTGGTTG GGGAACTCCT GATACAAGTG 
GACCGACTGA GGCTGAGATA CCAGAGAATG CTGCGGGACC GGGAGAAGGA CCGGGGCCCT 
AAGCCAGGGG ACATGGACTC CTGCCCAGCC CCACACCTAC TGTTGGGAGC AGTCTTCACG 
GAGGCTGAAA AGGCCAGGAT AGAAAGTTCT CCCAAACCCT TCTGTGAAGG AGAGAAGTTT 
TACATACCCC TGCAGAGACT GCTCACTTAC CCCAAACTGC ACCGCCTGTG TGCTGATGAG 
GACAGGGTGC GGTCCCTCAT TGCTGACTCG GTTTGCTTAA GCAGTGATGG CTCCGCAGTC 
GTGGTTGACC TGCATAATTC TTGGGATTAT CGGCCGGAAG AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072114.1
CDS	158..1345
Translation	MEMAESIPCNSVVGGNFKEVSPEKVIRFKPPLYKQRYQFVRDLVDRHEPKKVADLGCGDAKLLKLLKIYPCIQLLVGVDINEEKLHSNGHRLSPYLGEFVKPRDLDLTVTLYHGSVVERDSRLLGFDLITCIELIEHLDSDDLARFPDVVFGYLSPAMVVISTPNAEFNPLFPTVTLRDADHKFEWSRMEFQTWALHVANCYNYRVEFTGVGTPPAGSEHVGYCTQIGVFTKNGGKLSKPSVSQQCDQHVYKPVYTTSYPSLQQEKVLKFVLVGELLIQVDRLRLRYQRMLRDREKDRGPKPGDMDSCPAPHLLLGAVFTEAEKARIESSPKPFCEGEKFYIPLQRLLTYPKLHRLCADEDRVRSLIADSVCLSSDGSAVVVDLHNSWDYRPEEN

Target ORF information:

RefSeq Version	NM_001078646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078646.1

ORF Insert Sequence:

ATGGAAATGG CAGAAAGCAT ACCGTGCAAT AGTGTGGTTG GTGGCAATTT TAAAGAAGTT 
AGTCCAGAGA AGGTAATTCG GTTCAAACCA CCATTATACA AACAACGGTA TCAGTTTGTT 
AGAGATTTAG TGGATCGCCA TGAACCCAAG AAGGTTGCCG ACCTGGGATG TGGAGATGCC 
AAGCTCCTAA AGCTGCTAAA AATCTACCCA TGCATTCAAC TGCTTGTTGG GGTGGATATC 
AATGAAGAAA AATTGCATTC AAATGGGCAT CGCTTGTCTC CCTATTTGGG GGAGTTTGTA 
AAACCCCGAG ATCTAGATTT GACTGTCACC TTGTATCATG GCTCTGTTGT GGAGAGAGAC 
TCCCGCTTGC TTGGATTCGA TTTGATAACA TGCATTGAAT TAATAGAACA CTTGGATTCA 
GATGATCTGG CCAGATTTCC TGATGTGGTT TTTGGATACC TGTCACCAGC CATGGTTGTC 
ATCAGCACCC CAAATGCTGA ATTCAACCCC CTGTTTCCTA CAGTCACCCT GAGGGACGCC 
GACCATAAAT TTGAGTGGAG CAGAATGGAG TTTCAGACCT GGGCTTTACA CGTGGCAAAC 
TGCTACAATT ACCGTGTGGA GTTTACTGGG GTAGGGACAC CGCCAGCTGG ATCTGAGCAT 
GTTGGATATT GTACCCAGAT AGGTGTCTTT ACAAAGAATG GCGGGAAGCT CTCTAAACCT 
TCGGTTTCCC AGCAGTGTGA CCAGCACGTT TATAAACCTG TTTATACAAC CTCCTACCCG 
AGTTTACAGC AGGAGAAGGT GCTCAAGTTT GTTCTGGTTG GGGAACTCCT GATACAAGTG 
GACCGACTGA GGCTGAGATA CCAGAGAATG CTGCGGGACC GGGAGAAGGA CCGGGGCCCT 
AAGCCAGGGG ACATGGACTC CTGCCCAGCC CCACACCTAC TGTTGGGAGC AGTCTTCACG 
GAGGCTGAAA AGGCCAGGAT AGAAAGTTCT CCCAAACCCT TCTGTGAAGG AGAGAAGTTT 
TACATACCCC TGCAGAGACT GCTCACTTAC CCCAAACTGC ACCGCCTGTG TGCTGATGAG 
GACAGGGTGC GGTCCCTCAT TGCTGACTCG GTTTGCTTAA GCAGTGATGG CTCCGCAGTC 
GTGGTTGACC TGCATAATTC TTGGGATTAT CGGCCGGAAG AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Henmt1 ( NM_025723.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079999.2 Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00935
Clone ID Related Accession (Same CDS sequence)	NM_001078646.1 , NM_025723.2
Accession Version	NM_025723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	small RNA 2'-O-methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV650789.1 and BC099408.1. On Nov 25, 2006 this sequence version replaced NM_025723.1. ##Evidence-Data-START## Transcript exon combination :: AK085587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGG CAGAAAGCAT ACCGTGCAAT AGTGTGGTTG GTGGCAATTT TAAAGAAGTT 
AGTCCAGAGA AGGTAATTCG GTTCAAACCA CCATTATACA AACAACGGTA TCAGTTTGTT 
AGAGATTTAG TGGATCGCCA TGAACCCAAG AAGGTTGCCG ACCTGGGATG TGGAGATGCC 
AAGCTCCTAA AGCTGCTAAA AATCTACCCA TGCATTCAAC TGCTTGTTGG GGTGGATATC 
AATGAAGAAA AATTGCATTC AAATGGGCAT CGCTTGTCTC CCTATTTGGG GGAGTTTGTA 
AAACCCCGAG ATCTAGATTT GACTGTCACC TTGTATCATG GCTCTGTTGT GGAGAGAGAC 
TCCCGCTTGC TTGGATTCGA TTTGATAACA TGCATTGAAT TAATAGAACA CTTGGATTCA 
GATGATCTGG CCAGATTTCC TGATGTGGTT TTTGGATACC TGTCACCAGC CATGGTTGTC 
ATCAGCACCC CAAATGCTGA ATTCAACCCC CTGTTTCCTA CAGTCACCCT GAGGGACGCC 
GACCATAAAT TTGAGTGGAG CAGAATGGAG TTTCAGACCT GGGCTTTACA CGTGGCAAAC 
TGCTACAATT ACCGTGTGGA GTTTACTGGG GTAGGGACAC CGCCAGCTGG ATCTGAGCAT 
GTTGGATATT GTACCCAGAT AGGTGTCTTT ACAAAGAATG GCGGGAAGCT CTCTAAACCT 
TCGGTTTCCC AGCAGTGTGA CCAGCACGTT TATAAACCTG TTTATACAAC CTCCTACCCG 
AGTTTACAGC AGGAGAAGGT GCTCAAGTTT GTTCTGGTTG GGGAACTCCT GATACAAGTG 
GACCGACTGA GGCTGAGATA CCAGAGAATG CTGCGGGACC GGGAGAAGGA CCGGGGCCCT 
AAGCCAGGGG ACATGGACTC CTGCCCAGCC CCACACCTAC TGTTGGGAGC AGTCTTCACG 
GAGGCTGAAA AGGCCAGGAT AGAAAGTTCT CCCAAACCCT TCTGTGAAGG AGAGAAGTTT 
TACATACCCC TGCAGAGACT GCTCACTTAC CCCAAACTGC ACCGCCTGTG TGCTGATGAG 
GACAGGGTGC GGTCCCTCAT TGCTGACTCG GTTTGCTTAA GCAGTGATGG CTCCGCAGTC 
GTGGTTGACC TGCATAATTC TTGGGATTAT CGGCCGGAAG AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079999.2
CDS	473..1660
Translation	MEMAESIPCNSVVGGNFKEVSPEKVIRFKPPLYKQRYQFVRDLVDRHEPKKVADLGCGDAKLLKLLKIYPCIQLLVGVDINEEKLHSNGHRLSPYLGEFVKPRDLDLTVTLYHGSVVERDSRLLGFDLITCIELIEHLDSDDLARFPDVVFGYLSPAMVVISTPNAEFNPLFPTVTLRDADHKFEWSRMEFQTWALHVANCYNYRVEFTGVGTPPAGSEHVGYCTQIGVFTKNGGKLSKPSVSQQCDQHVYKPVYTTSYPSLQQEKVLKFVLVGELLIQVDRLRLRYQRMLRDREKDRGPKPGDMDSCPAPHLLLGAVFTEAEKARIESSPKPFCEGEKFYIPLQRLLTYPKLHRLCADEDRVRSLIADSVCLSSDGSAVVVDLHNSWDYRPEEN

Target ORF information:

RefSeq Version	NM_025723.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025723.2

ORF Insert Sequence:

ATGGAAATGG CAGAAAGCAT ACCGTGCAAT AGTGTGGTTG GTGGCAATTT TAAAGAAGTT 
AGTCCAGAGA AGGTAATTCG GTTCAAACCA CCATTATACA AACAACGGTA TCAGTTTGTT 
AGAGATTTAG TGGATCGCCA TGAACCCAAG AAGGTTGCCG ACCTGGGATG TGGAGATGCC 
AAGCTCCTAA AGCTGCTAAA AATCTACCCA TGCATTCAAC TGCTTGTTGG GGTGGATATC 
AATGAAGAAA AATTGCATTC AAATGGGCAT CGCTTGTCTC CCTATTTGGG GGAGTTTGTA 
AAACCCCGAG ATCTAGATTT GACTGTCACC TTGTATCATG GCTCTGTTGT GGAGAGAGAC 
TCCCGCTTGC TTGGATTCGA TTTGATAACA TGCATTGAAT TAATAGAACA CTTGGATTCA 
GATGATCTGG CCAGATTTCC TGATGTGGTT TTTGGATACC TGTCACCAGC CATGGTTGTC 
ATCAGCACCC CAAATGCTGA ATTCAACCCC CTGTTTCCTA CAGTCACCCT GAGGGACGCC 
GACCATAAAT TTGAGTGGAG CAGAATGGAG TTTCAGACCT GGGCTTTACA CGTGGCAAAC 
TGCTACAATT ACCGTGTGGA GTTTACTGGG GTAGGGACAC CGCCAGCTGG ATCTGAGCAT 
GTTGGATATT GTACCCAGAT AGGTGTCTTT ACAAAGAATG GCGGGAAGCT CTCTAAACCT 
TCGGTTTCCC AGCAGTGTGA CCAGCACGTT TATAAACCTG TTTATACAAC CTCCTACCCG 
AGTTTACAGC AGGAGAAGGT GCTCAAGTTT GTTCTGGTTG GGGAACTCCT GATACAAGTG 
GACCGACTGA GGCTGAGATA CCAGAGAATG CTGCGGGACC GGGAGAAGGA CCGGGGCCCT 
AAGCCAGGGG ACATGGACTC CTGCCCAGCC CCACACCTAC TGTTGGGAGC AGTCTTCACG 
GAGGCTGAAA AGGCCAGGAT AGAAAGTTCT CCCAAACCCT TCTGTGAAGG AGAGAAGTTT 
TACATACCCC TGCAGAGACT GCTCACTTAC CCCAAACTGC ACCGCCTGTG TGCTGATGAG 
GACAGGGTGC GGTCCCTCAT TGCTGACTCG GTTTGCTTAA GCAGTGATGG CTCCGCAGTC 
GTGGTTGACC TGCATAATTC TTGGGATTAT CGGCCGGAAG AGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Henmt1 ( XM_006501898.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501961.1 Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32177
Clone ID Related Accession (Same CDS sequence)	XM_006501898.2 , XM_006501898.3
Accession Version	XM_006501898.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	small RNA 2'-O-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGG CAGAAAGCAT ACCGGTTGCC GACCTGGGAT GTGGAGATGC CAAGCTCCTA 
AAGCTGCTAA AAATCTACCC ATGCATTCAA CTGCTTGTTG GGGTGGATAT CAATGAAGAA 
AAATTGCATT CAAATGGGCA TCGCTTGTCT CCCTATTTGG GGGAGTTTGT AAAACCCCGA 
GATCTAGATT TGACTGTCAC CTTGTATCAT GGCTCTGTTG TGGAGAGAGA CTCCCGCTTG 
CTTGGATTCG ATTTGATAAC ATGCATTGAA TTAATAGAAC ACTTGGATTC AGATGATCTG 
GCCAGATTTC CTGATGTGGT TTTTGGATAC CTGTCACCAG CCATGGTTGT CATCAGCACC 
CCAAATGCTG AATTCAACCC CCTGTTTCCT ACAGTCACCC TGAGGGACGC CGACCATAAA 
TTTGAGTGGA GCAGAATGGA GTTTCAGACC TGGGCTTTAC ACGTGGCAAA CTGCTACAAT 
TACCGTGTGG AGTTTACTGG GGTAGGGACA CCGCCAGCTG GATCTGAGCA TGTTGGATAT 
TGTACCCAGA TAGGTGTCTT TACAAAGAAT GGCGGGAAGC TCTCTAAACC TTCGGTTTCC 
CAGCAGTGTG ACCAGCACGT TTATAAACCT GTTTATACAA CCTCCTACCC GAGTTTACAG 
CAGGAGAAGG TGCTCAAGTT TGTTCTGGTT GGGGAACTCC TGATACAAGT GGACCGACTG 
AGGCTGAGAT ACCAGAGAAT GCTGCGGGAC CGGGAGAAGG ACCGGGGCCC TAAGCCAGGG 
GACATGGACT CCTGCCCAGC CCCACACCTA CTGTTGGGAG CAGTCTTCAC GGAGGCTGAA 
AAGGCCAGGA TAGAAAGTTC TCCCAAACCC TTCTGTGAAG GAGAGAAGTT TTACATACCC 
CTGCAGAGAC TGCTCACTTA CCCCAAACTG CACCGCCTGT GTGCTGATGA GGACAGGGTG 
CGGTCCCTCA TTGCTGACTC GGTTTGCTTA AGCAGTGATG GCTCCGCAGT CGTGGTTGAC 
CTGCATAATT CTTGGGATTA TCGGCCGGAA GAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501961.1
CDS	2014..3072
Translation	MEMAESIPVADLGCGDAKLLKLLKIYPCIQLLVGVDINEEKLHSNGHRLSPYLGEFVKPRDLDLTVTLYHGSVVERDSRLLGFDLITCIELIEHLDSDDLARFPDVVFGYLSPAMVVISTPNAEFNPLFPTVTLRDADHKFEWSRMEFQTWALHVANCYNYRVEFTGVGTPPAGSEHVGYCTQIGVFTKNGGKLSKPSVSQQCDQHVYKPVYTTSYPSLQQEKVLKFVLVGELLIQVDRLRLRYQRMLRDREKDRGPKPGDMDSCPAPHLLLGAVFTEAEKARIESSPKPFCEGEKFYIPLQRLLTYPKLHRLCADEDRVRSLIADSVCLSSDGSAVVVDLHNSWDYRPEEN

Target ORF information:

RefSeq Version	XM_006501898.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501898.2

ORF Insert Sequence:

ATGGAAATGG CAGAAAGCAT ACCGGTTGCC GACCTGGGAT GTGGAGATGC CAAGCTCCTA 
AAGCTGCTAA AAATCTACCC ATGCATTCAA CTGCTTGTTG GGGTGGATAT CAATGAAGAA 
AAATTGCATT CAAATGGGCA TCGCTTGTCT CCCTATTTGG GGGAGTTTGT AAAACCCCGA 
GATCTAGATT TGACTGTCAC CTTGTATCAT GGCTCTGTTG TGGAGAGAGA CTCCCGCTTG 
CTTGGATTCG ATTTGATAAC ATGCATTGAA TTAATAGAAC ACTTGGATTC AGATGATCTG 
GCCAGATTTC CTGATGTGGT TTTTGGATAC CTGTCACCAG CCATGGTTGT CATCAGCACC 
CCAAATGCTG AATTCAACCC CCTGTTTCCT ACAGTCACCC TGAGGGACGC CGACCATAAA 
TTTGAGTGGA GCAGAATGGA GTTTCAGACC TGGGCTTTAC ACGTGGCAAA CTGCTACAAT 
TACCGTGTGG AGTTTACTGG GGTAGGGACA CCGCCAGCTG GATCTGAGCA TGTTGGATAT 
TGTACCCAGA TAGGTGTCTT TACAAAGAAT GGCGGGAAGC TCTCTAAACC TTCGGTTTCC 
CAGCAGTGTG ACCAGCACGT TTATAAACCT GTTTATACAA CCTCCTACCC GAGTTTACAG 
CAGGAGAAGG TGCTCAAGTT TGTTCTGGTT GGGGAACTCC TGATACAAGT GGACCGACTG 
AGGCTGAGAT ACCAGAGAAT GCTGCGGGAC CGGGAGAAGG ACCGGGGCCC TAAGCCAGGG 
GACATGGACT CCTGCCCAGC CCCACACCTA CTGTTGGGAG CAGTCTTCAC GGAGGCTGAA 
AAGGCCAGGA TAGAAAGTTC TCCCAAACCC TTCTGTGAAG GAGAGAAGTT TTACATACCC 
CTGCAGAGAC TGCTCACTTA CCCCAAACTG CACCGCCTGT GTGCTGATGA GGACAGGGTG 
CGGTCCCTCA TTGCTGACTC GGTTTGCTTA AGCAGTGATG GCTCCGCAGT CGTGGTTGAC 
CTGCATAATT CTTGGGATTA TCGGCCGGAA GAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Henmt1 ( XM_006501898.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501961.1 Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32177
Clone ID Related Accession (Same CDS sequence)	XM_006501898.2 , XM_006501898.3
Accession Version	XM_006501898.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	small RNA 2'-O-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501898.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGG CAGAAAGCAT ACCGGTTGCC GACCTGGGAT GTGGAGATGC CAAGCTCCTA 
AAGCTGCTAA AAATCTACCC ATGCATTCAA CTGCTTGTTG GGGTGGATAT CAATGAAGAA 
AAATTGCATT CAAATGGGCA TCGCTTGTCT CCCTATTTGG GGGAGTTTGT AAAACCCCGA 
GATCTAGATT TGACTGTCAC CTTGTATCAT GGCTCTGTTG TGGAGAGAGA CTCCCGCTTG 
CTTGGATTCG ATTTGATAAC ATGCATTGAA TTAATAGAAC ACTTGGATTC AGATGATCTG 
GCCAGATTTC CTGATGTGGT TTTTGGATAC CTGTCACCAG CCATGGTTGT CATCAGCACC 
CCAAATGCTG AATTCAACCC CCTGTTTCCT ACAGTCACCC TGAGGGACGC CGACCATAAA 
TTTGAGTGGA GCAGAATGGA GTTTCAGACC TGGGCTTTAC ACGTGGCAAA CTGCTACAAT 
TACCGTGTGG AGTTTACTGG GGTAGGGACA CCGCCAGCTG GATCTGAGCA TGTTGGATAT 
TGTACCCAGA TAGGTGTCTT TACAAAGAAT GGCGGGAAGC TCTCTAAACC TTCGGTTTCC 
CAGCAGTGTG ACCAGCACGT TTATAAACCT GTTTATACAA CCTCCTACCC GAGTTTACAG 
CAGGAGAAGG TGCTCAAGTT TGTTCTGGTT GGGGAACTCC TGATACAAGT GGACCGACTG 
AGGCTGAGAT ACCAGAGAAT GCTGCGGGAC CGGGAGAAGG ACCGGGGCCC TAAGCCAGGG 
GACATGGACT CCTGCCCAGC CCCACACCTA CTGTTGGGAG CAGTCTTCAC GGAGGCTGAA 
AAGGCCAGGA TAGAAAGTTC TCCCAAACCC TTCTGTGAAG GAGAGAAGTT TTACATACCC 
CTGCAGAGAC TGCTCACTTA CCCCAAACTG CACCGCCTGT GTGCTGATGA GGACAGGGTG 
CGGTCCCTCA TTGCTGACTC GGTTTGCTTA AGCAGTGATG GCTCCGCAGT CGTGGTTGAC 
CTGCATAATT CTTGGGATTA TCGGCCGGAA GAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501961.1
CDS	1103..2161
Translation	MEMAESIPVADLGCGDAKLLKLLKIYPCIQLLVGVDINEEKLHSNGHRLSPYLGEFVKPRDLDLTVTLYHGSVVERDSRLLGFDLITCIELIEHLDSDDLARFPDVVFGYLSPAMVVISTPNAEFNPLFPTVTLRDADHKFEWSRMEFQTWALHVANCYNYRVEFTGVGTPPAGSEHVGYCTQIGVFTKNGGKLSKPSVSQQCDQHVYKPVYTTSYPSLQQEKVLKFVLVGELLIQVDRLRLRYQRMLRDREKDRGPKPGDMDSCPAPHLLLGAVFTEAEKARIESSPKPFCEGEKFYIPLQRLLTYPKLHRLCADEDRVRSLIADSVCLSSDGSAVVVDLHNSWDYRPEEN

Target ORF information:

RefSeq Version	XM_006501898.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501898.3

ORF Insert Sequence:

ATGGAAATGG CAGAAAGCAT ACCGGTTGCC GACCTGGGAT GTGGAGATGC CAAGCTCCTA 
AAGCTGCTAA AAATCTACCC ATGCATTCAA CTGCTTGTTG GGGTGGATAT CAATGAAGAA 
AAATTGCATT CAAATGGGCA TCGCTTGTCT CCCTATTTGG GGGAGTTTGT AAAACCCCGA 
GATCTAGATT TGACTGTCAC CTTGTATCAT GGCTCTGTTG TGGAGAGAGA CTCCCGCTTG 
CTTGGATTCG ATTTGATAAC ATGCATTGAA TTAATAGAAC ACTTGGATTC AGATGATCTG 
GCCAGATTTC CTGATGTGGT TTTTGGATAC CTGTCACCAG CCATGGTTGT CATCAGCACC 
CCAAATGCTG AATTCAACCC CCTGTTTCCT ACAGTCACCC TGAGGGACGC CGACCATAAA 
TTTGAGTGGA GCAGAATGGA GTTTCAGACC TGGGCTTTAC ACGTGGCAAA CTGCTACAAT 
TACCGTGTGG AGTTTACTGG GGTAGGGACA CCGCCAGCTG GATCTGAGCA TGTTGGATAT 
TGTACCCAGA TAGGTGTCTT TACAAAGAAT GGCGGGAAGC TCTCTAAACC TTCGGTTTCC 
CAGCAGTGTG ACCAGCACGT TTATAAACCT GTTTATACAA CCTCCTACCC GAGTTTACAG 
CAGGAGAAGG TGCTCAAGTT TGTTCTGGTT GGGGAACTCC TGATACAAGT GGACCGACTG 
AGGCTGAGAT ACCAGAGAAT GCTGCGGGAC CGGGAGAAGG ACCGGGGCCC TAAGCCAGGG 
GACATGGACT CCTGCCCAGC CCCACACCTA CTGTTGGGAG CAGTCTTCAC GGAGGCTGAA 
AAGGCCAGGA TAGAAAGTTC TCCCAAACCC TTCTGTGAAG GAGAGAAGTT TTACATACCC 
CTGCAGAGAC TGCTCACTTA CCCCAAACTG CACCGCCTGT GTGCTGATGA GGACAGGGTG 
CGGTCCCTCA TTGCTGACTC GGTTTGCTTA AGCAGTGATG GCTCCGCAGT CGTGGTTGAC 
CTGCATAATT CTTGGGATTA TCGGCCGGAA GAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
The Journal of biological chemistry293(26)9981-9994(2018 06)
Peng L,Zhang F,Shang R,Wang X,Chen J,Chou JJ,Ma J,Wu L,Huang Y

HENMT1 and piRNA Stability Are Required for Adult Male Germ Cell Transposon Repression and to Define the Spermatogenic Program in the Mouse.
PLoS genetics11(10)e1005620(2015 Oct)
Lim SL,Qu ZP,Kortschak RD,Lawrence DM,Geoghegan J,Hempfling AL,Bergmann M,Goodnow CC,Ormandy CJ,Wong L,Mann J,Scott HS,Jamsai D,Adelson DL,O'Bryan MK

The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs.
RNA (New York, N.Y.)13(9)1397-401(2007 Sep)
Kirino Y,Mourelatos Z

2'-O-methyl modification in mouse piRNAs and its methylase.
Nucleic acids symposium series (2004)(51)417-8(2007)
Kirino Y,Mourelatos Z

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

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Henmt1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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