Asf1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asf1a
Entrez Gene ID	66403
Full Name	anti-silencing function 1A histone chaperone
Synonyms	2310079C17Rik,C79052
General protein information	Preferred Names anti-silencing function 1A histone chaperone Names histone chaperone ASF1A ASF1 anti-silencing function 1 homolog A anti-silencing function protein 1 homolog A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025541.3	NP_079817.1	histone chaperone ASF1A

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ASF1	NP_012420.1
Drosophila melanogaster (fruit fly)	asf1	NP_001189131.1
Bos taurus (cattle)	ASF1A	NP_001069961.1
Mus musculus (house mouse)	Asf1a	NP_079817.1
Gallus gallus (chicken)	ASF1A	NP_001038155.1
Danio rerio (zebrafish)	asf1ba	NP_996946.1
Xenopus tropicalis (tropical clawed frog)	asf1b	NP_001004974.1
Danio rerio (zebrafish)	LOC100535896	XP_003198211.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005920	XP_315950.4
Homo sapiens (human)	ASF1A	NP_054753.1
Rattus norvegicus (Norway rat)	Asf1a	NP_001099859.1
Caenorhabditis elegans (roundworm)	asfl-1	NP_492567.3
Caenorhabditis elegans (roundworm)	unc-85	NP_494837.2
Arabidopsis thaliana (thale cress)	SGA2	NP_176846.1
Pan troglodytes (chimpanzee)	ASF1A	XP_003311502.1
Macaca mulatta (Rhesus monkey)	ASF1A	XP_001110511.1
Danio rerio (zebrafish)	asf1bb	NP_999922.1
Arabidopsis thaliana (thale cress)	ASF1B	NP_198627.1
Oryza sativa (rice)	Os05g0553800	NP_001056265.1
Canis lupus familiaris (dog)	ASF1A	XP_533479.1

Gene Ontology
Bibliography

Related articles in PubMed

Asf1a resolves bivalent chromatin domains for the induction of lineage-specific genes during mouse embryonic stem cell differentiation.
Gao Y, Gan H, Lou Z, Zhang Z
Proceedings of the National Academy of Sciences of the United States of America115(27)E6162-E6171(2018 07)

Differential requirements for Tousled-like kinases 1 and 2 in mammalian development.
Segura-Bayona S, Knobel PA, González-Burón H, Youssef SA, Peña-Blanco A, Coyaud É, López-Rovira T, Rein K, Palenzuela L, Colombelli J, Forrow S, Raught B, Groth A, de Bruin A, Stracker TH
Cell death and differentiation24(11)1872-1885(2017 11)

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Asf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23411	NM_025541.3 Latest version!	Mus musculus anti-silencing function 1A histone chaperone (Asf1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asf1a ( NM_025541.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079817.1 Mus musculus anti-silencing function 1A histone chaperone (Asf1a), mRNA.

Price & Availability↑

CloneID	OMu23411
Clone ID Related Accession (Same CDS sequence)	NM_025541.3
Accession Version	NM_025541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	histone chaperone ASF1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010210.1 and AC153949.3. On Oct 5, 2005 this sequence version replaced NM_025541.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088618.1, AK031513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCGTCGCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAGTGG 
AAAATAATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CCGTTCCTGC AGGAAGGCAT ATGTTTGTGT TTCAGGCTGA TGCACCGAAT 
GCAGGACTCA TCCCAGATGC AGATGCAGTG GGGGTAACAG TTGTTCTGAT TACTTGCACC 
TACCGAGGTC AAGAATTTAT TAGAGTTGGC TACTATGTAA ATAATGAATA CACTGAAACA 
GAATTAAGGG AAAACCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGAGTCAC AAGATTCCAC ATTAACTGGG AAGATAATAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA CCCAAATCTA CAGTCCCTTC TTTCAACAGA TGCATTACCG 
TCAGCGTCAA AGGGATGGTC CACGTCAGAA AACTCACTCA ATGTTATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079817.1
CDS	230..844
Translation	MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNAGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

Target ORF information:

RefSeq Version	NM_025541.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anti-silencing function 1A histone chaperone (Asf1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025541.3

ORF Insert Sequence:

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCGTCGCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAGTGG 
AAAATAATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CCGTTCCTGC AGGAAGGCAT ATGTTTGTGT TTCAGGCTGA TGCACCGAAT 
GCAGGACTCA TCCCAGATGC AGATGCAGTG GGGGTAACAG TTGTTCTGAT TACTTGCACC 
TACCGAGGTC AAGAATTTAT TAGAGTTGGC TACTATGTAA ATAATGAATA CACTGAAACA 
GAATTAAGGG AAAACCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGAGTCAC AAGATTCCAC ATTAACTGGG AAGATAATAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA CCCAAATCTA CAGTCCCTTC TTTCAACAGA TGCATTACCG 
TCAGCGTCAA AGGGATGGTC CACGTCAGAA AACTCACTCA ATGTTATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Asf1a resolves bivalent chromatin domains for the induction of lineage-specific genes during mouse embryonic stem cell differentiation.
Proceedings of the National Academy of Sciences of the United States of America115(27)E6162-E6171(2018 07)
Gao Y,Gan H,Lou Z,Zhang Z

Differential requirements for Tousled-like kinases 1 and 2 in mammalian development.
Cell death and differentiation24(11)1872-1885(2017 11)
Segura-Bayona S,Knobel PA,González-Burón H,Youssef SA,Peña-Blanco A,Coyaud É,López-Rovira T,Rein K,Palenzuela L,Colombelli J,Forrow S,Raught B,Groth A,de Bruin A,Stracker TH

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Asf1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM