Ndufb5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufb5
Entrez Gene ID	66046
Full Name	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5
Synonyms	0610007D05Rik,AU015782,CI-SGDH,SGDH
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial NADH-ubiquinone oxidoreductase SGDH subunit complex I-SGDH
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025316.2	NP_079592.2	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial precursor
XM_006535514.2	XP_006535577.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial isoform X1
XM_006535514.3	XP_006535577.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007297	XP_308524.4
Bos taurus (cattle)	NDUFB5	NP_788829.1
Homo sapiens (human)	NDUFB5	NP_002483.1
Danio rerio (zebrafish)	ndufb5	NP_001099066.1
Canis lupus familiaris (dog)	NDUFB5	XP_535812.1
Canis lupus familiaris (dog)	LOC102154590	XP_005615552.1
Macaca mulatta (Rhesus monkey)	NDUFB5	XP_001107947.1
Drosophila melanogaster (fruit fly)	l(3)neo18	NP_001261742.1
Pan troglodytes (chimpanzee)	NDUFB5	NP_001065274.1
Mus musculus (house mouse)	Ndufb5	NP_079592.2
Rattus norvegicus (Norway rat)	Ndufb5	NP_001099896.1
Gallus gallus (chicken)	NDUFB5	NP_001006553.1
Xenopus tropicalis (tropical clawed frog)	ndufb5	NP_001017303.1

Gene Ontology
Bibliography

Related articles in PubMed

Chronic Activation of γ2 AMPK Induces Obesity and Reduces β Cell Function.
Yavari A, Stocker CJ, Ghaffari S, Wargent ET, Steeples V, Czibik G, Pinter K, Bellahcene M, Woods A, Martínez de Morentin PB, Cansell C, Lam BY, Chuster A, Petkevicius K, Nguyen-Tu MS, Martinez-Sanchez A, Pullen TJ, Oliver PL, Stockenhuber A, Nguyen C, Lazdam M, O'Dowd JF, Harikumar P, Tóth M, Beall C, Kyriakou T, Parnis J, Sarma D, Katritsis G, Wortmann DD, Harper AR, Brown LA, Willows R, Gandra S, Poncio V, de Oliveira Figueiredo MJ, Qi NR, Peirson SN, McCrimmon RJ, Gereben B, Tretter L, Fekete C, Redwood C, Yeo GS, Heisler LK, Rutter GA, Smith MA, Withers DJ, Carling D, Sternick EB, Arch JR, Cawthorne MA, Watkins H, Ashrafian H
Cell metabolism23(5)821-36(2016 May)

Region-specific expression of mitochondrial complex I genes during murine brain development.
Wirtz S, Schuelke M
PloS one6(4)e18897(2011 Apr)

Identification of transcripts with enriched expression in the developing and adult pancreas.
Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD
Genome biology9(6)R99(2008)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Proteomic analysis of the mouse liver mitochondrial inner membrane.
Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC
The Journal of biological chemistry278(42)41566-71(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndufb5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufb5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31409	XM_006535514.3 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu18530	NM_025316.2 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OMu31409	XM_006535514.2	Mus musculus NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 (Ndufb5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufb5 ( XM_006535514.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535577.1 Mus musculus NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 (Ndufb5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31409
Clone ID Related Accession (Same CDS sequence)	XM_006535514.2 , XM_006535514.3
Accession Version	XM_006535514.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCT ACCTTATGTT GACTGGGATC CCAGTTATAA TTGGCATAAC ACTGGTGAAC 
ATATTTATTG GTGAAGCCGA GCTTGCAGAA ATCCCAGAAG GCTACATCCC TGAACACTGG 
GAGTATTACA AGCATCCGAT ATCAAGATGG ATCGCCCGGA ATTTCTATGA TGGCCCTGAA 
AAGAACTACG AGAAAACCCT GGCTATCCTC CAGATTGAAT CTGAAAAGGC TGAGTTACGG 
TTAAAGGAGC AGGAAGTTCG AAGGCTGATG CGTGCCCGAG GTGATGGACC CTGGTATCAA 
TTTCCAACCC CTGAAAAGGA GTTCATTGAT CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535577.1
CDS	211..570
Translation	MKFYLMLTGIPVIIGITLVNIFIGEAELAEIPEGYIPEHWEYYKHPISRWIARNFYDGPEKNYEKTLAILQIESEKAELRLKEQEVRRLMRARGDGPWYQFPTPEKEFIDHSPKATPDN

Target ORF information:

RefSeq Version	XM_006535514.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 (Ndufb5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535514.2

ORF Insert Sequence:

ATGAAATTCT ACCTTATGTT GACTGGGATC CCAGTTATAA TTGGCATAAC ACTGGTGAAC 
ATATTTATTG GTGAAGCCGA GCTTGCAGAA ATCCCAGAAG GCTACATCCC TGAACACTGG 
GAGTATTACA AGCATCCGAT ATCAAGATGG ATCGCCCGGA ATTTCTATGA TGGCCCTGAA 
AAGAACTACG AGAAAACCCT GGCTATCCTC CAGATTGAAT CTGAAAAGGC TGAGTTACGG 
TTAAAGGAGC AGGAAGTTCG AAGGCTGATG CGTGCCCGAG GTGATGGACC CTGGTATCAA 
TTTCCAACCC CTGAAAAGGA GTTCATTGAT CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufb5 ( XM_006535514.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535577.1 Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31409
Clone ID Related Accession (Same CDS sequence)	XM_006535514.2 , XM_006535514.3
Accession Version	XM_006535514.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535514.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCT ACCTTATGTT GACTGGGATC CCAGTTATAA TTGGCATAAC ACTGGTGAAC 
ATATTTATTG GTGAAGCCGA GCTTGCAGAA ATCCCAGAAG GCTACATCCC TGAACACTGG 
GAGTATTACA AGCATCCGAT ATCAAGATGG ATCGCCCGGA ATTTCTATGA TGGCCCTGAA 
AAGAACTACG AGAAAACCCT GGCTATCCTC CAGATTGAAT CTGAAAAGGC TGAGTTACGG 
TTAAAGGAGC AGGAAGTTCG AAGGCTGATG CGTGCCCGAG GTGATGGACC CTGGTATCAA 
TTTCCAACCC CTGAAAAGGA GTTCATTGAT CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535577.1
CDS	261..620
Translation	MKFYLMLTGIPVIIGITLVNIFIGEAELAEIPEGYIPEHWEYYKHPISRWIARNFYDGPEKNYEKTLAILQIESEKAELRLKEQEVRRLMRARGDGPWYQFPTPEKEFIDHSPKATPDN

Target ORF information:

RefSeq Version	XM_006535514.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535514.3

ORF Insert Sequence:

ATGAAATTCT ACCTTATGTT GACTGGGATC CCAGTTATAA TTGGCATAAC ACTGGTGAAC 
ATATTTATTG GTGAAGCCGA GCTTGCAGAA ATCCCAGAAG GCTACATCCC TGAACACTGG 
GAGTATTACA AGCATCCGAT ATCAAGATGG ATCGCCCGGA ATTTCTATGA TGGCCCTGAA 
AAGAACTACG AGAAAACCCT GGCTATCCTC CAGATTGAAT CTGAAAAGGC TGAGTTACGG 
TTAAAGGAGC AGGAAGTTCG AAGGCTGATG CGTGCCCGAG GTGATGGACC CTGGTATCAA 
TTTCCAACCC CTGAAAAGGA GTTCATTGAT CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufb5 ( NM_025316.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079592.2 Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), mRNA.

Price & Availability↑

CloneID	OMu18530
Clone ID Related Accession (Same CDS sequence)	NM_025316.2
Accession Version	NM_025316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK004372.1. On or before Dec 7, 2005 this sequence version replaced XM_912457.1, NM_025316.1. ##Evidence-Data-START## Transcript exon combination :: AK004372.1, AK002279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCCA TGAGCCTGCT GCAGCGGGCT TCGGTCTCCG CGCTGACAGC GCTGTCTTGC 
CGCCGCGCTG GTCCTAGACT CGGAGTCGGA AGTTTTCTCA CGCGGAGCTT TCCGAAGACT 
GTCGCTCCTG TGCGGCACAG TGGGGACCAT GGCAAGAGAC TGTTTGTCGT CAAGCCTTCT 
TTATACTATG ACGCTCGCTT CTTGAGGTTA ATGAAATTCT ACCTTATGTT GACTGGGATC 
CCAGTTATAA TTGGCATAAC ACTGGTGAAC ATATTTATTG GTGAAGCCGA GCTTGCAGAA 
ATCCCAGAAG GCTACATCCC TGAACACTGG GAGTATTACA AGCATCCGAT ATCAAGATGG 
ATCGCCCGGA ATTTCTATGA TGGCCCTGAA AAGAACTACG AGAAAACCCT GGCTATCCTC 
CAGATTGAAT CTGAAAAGGC TGAGTTACGG TTAAAGGAGC AGGAAGTTCG AAGGCTGATG 
CGTGCCCGAG GTGATGGACC CTGGTATCAA TTTCCAACCC CTGAAAAGGA GTTCATTGAT 
CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079592.2
CDS	20..589
Translation	MAAMSLLQRASVSALTALSCRRAGPRLGVGSFLTRSFPKTVAPVRHSGDHGKRLFVVKPSLYYDARFLRLMKFYLMLTGIPVIIGITLVNIFIGEAELAEIPEGYIPEHWEYYKHPISRWIARNFYDGPEKNYEKTLAILQIESEKAELRLKEQEVRRLMRARGDGPWYQFPTPEKEFIDHSPKATPDN

Target ORF information:

RefSeq Version	NM_025316.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase subunit B5 (Ndufb5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025316.2

ORF Insert Sequence:

ATGGCGGCCA TGAGCCTGCT GCAGCGGGCT TCGGTCTCCG CGCTGACAGC GCTGTCTTGC 
CGCCGCGCTG GTCCTAGACT CGGAGTCGGA AGTTTTCTCA CGCGGAGCTT TCCGAAGACT 
GTCGCTCCTG TGCGGCACAG TGGGGACCAT GGCAAGAGAC TGTTTGTCGT CAAGCCTTCT 
TTATACTATG ACGCTCGCTT CTTGAGGTTA ATGAAATTCT ACCTTATGTT GACTGGGATC 
CCAGTTATAA TTGGCATAAC ACTGGTGAAC ATATTTATTG GTGAAGCCGA GCTTGCAGAA 
ATCCCAGAAG GCTACATCCC TGAACACTGG GAGTATTACA AGCATCCGAT ATCAAGATGG 
ATCGCCCGGA ATTTCTATGA TGGCCCTGAA AAGAACTACG AGAAAACCCT GGCTATCCTC 
CAGATTGAAT CTGAAAAGGC TGAGTTACGG TTAAAGGAGC AGGAAGTTCG AAGGCTGATG 
CGTGCCCGAG GTGATGGACC CTGGTATCAA TTTCCAACCC CTGAAAAGGA GTTCATTGAT 
CATTCTCCAA AGGCAACTCC TGACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chronic Activation of γ2 AMPK Induces Obesity and Reduces β Cell Function.
Cell metabolism23(5)821-36(2016 May)
Yavari A,Stocker CJ,Ghaffari S,Wargent ET,Steeples V,Czibik G,Pinter K,Bellahcene M,Woods A,Martínez de Morentin PB,Cansell C,Lam BY,Chuster A,Petkevicius K,Nguyen-Tu MS,Martinez-Sanchez A,Pullen TJ,Oliver PL,Stockenhuber A,Nguyen C,Lazdam M,O'Dowd JF,Harikumar P,Tóth M,Beall C,Kyriakou T,Parnis J,Sarma D,Katritsis G,Wortmann DD,Harper AR,Brown LA,Willows R,Gandra S,Poncio V,de Oliveira Figueiredo MJ,Qi NR,Peirson SN,McCrimmon RJ,Gereben B,Tretter L,Fekete C,Redwood C,Yeo GS,Heisler LK,Rutter GA,Smith MA,Withers DJ,Carling D,Sternick EB,Arch JR,Cawthorne MA,Watkins H,Ashrafian H

Region-specific expression of mitochondrial complex I genes during murine brain development.
PloS one6(4)e18897(2011 Apr)
Wirtz S,Schuelke M

Identification of transcripts with enriched expression in the developing and adult pancreas.
Genome biology9(6)R99(2008)
Hoffman BG,Zavaglia B,Witzsche J,Ruiz de Algara T,Beach M,Hoodless PA,Jones SJ,Marra MA,Helgason CD

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Proteomic analysis of the mouse liver mitochondrial inner membrane.
The Journal of biological chemistry278(42)41566-71(2003 Oct)
Da Cruz S,Xenarios I,Langridge J,Vilbois F,Parone PA,Martinou JC

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Ndufb5 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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