Mical2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mical2
Entrez Gene ID	320878
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms	5330438E18Rik,9530064J02,MICAL-2,mKIAA0750
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 2 Names [F-actin]-monooxygenase MICAL2 protein-methionine sulfoxide oxidase MICAL2 [F-actin]-methionine sulfoxide oxidase MICAL2 mMical2 microtubule associated monoxygenase, calponin and LIM domain containing 2 molecule interacting with CasL protein 2 protein MICAL-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001193305.1	NP_001180234.1	[F-actin]-monooxygenase MICAL2 isoform A
NM_177282.5	NP_796256.1	[F-actin]-monooxygenase MICAL2 isoform B
XM_006507954.2	XP_006508017.1	F-actin-monooxygenase MICAL2 isoform X1
XM_030242685.1	XP_030098545.1	F-actin-monooxygenase MICAL2 isoform X2
XM_006507954.1	XP_006508017.1	protein-methionine sulfoxide oxidase MICAL2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MICAL2	XP_005633730.1
Bos taurus (cattle)	MICAL2	XP_002693097.1
Pan troglodytes (chimpanzee)	MICAL2	XP_003951886.1
Mus musculus (house mouse)	Mical2	NP_001180234.1
Rattus norvegicus (Norway rat)	Mical2	NP_001132980.1
Gallus gallus (chicken)	MICAL2	XP_003641364.2
Xenopus tropicalis (tropical clawed frog)	LOC100487018	XP_002941765.2
Homo sapiens (human)	MICAL2	NP_055447.1
Danio rerio (zebrafish)	mical2b	NP_001258842.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-205-5p suppresses pulmonary vascular smooth muscle cell proliferation by targeting MICAL2-mediated Erk1/2 signaling.
Tao W, Sun W, Zhu H, Zhang J
Microvascular research12443-50(2019 07)

Actin stimulates reduction of the MICAL-2 monooxygenase domain.
McDonald CA, Liu YY, Palfey BA
Biochemistry52(35)6076-84(2013 Sep)

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Lee BC, Péterfi Z, Hoffmann FW, Moore RE, Kaya A, Avanesov A, Tarrago L, Zhou Y, Weerapana E, Fomenko DE, Hoffmann PR, Gladyshev VN
Molecular cell51(3)397-404(2013 Aug)

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map.
Elsen GE, Hodge RD, Bedogni F, Daza RA, Nelson BR, Shiba N, Reiner SL, Hevner RF
Proceedings of the National Academy of Sciences of the United States of America110(10)4081-6(2013 Mar)

MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion.
Terman JR, Mao T, Pasterkamp RJ, Yu HH, Kolodkin AL
Cell109(7)887-900(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mical2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mical2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06153	NM_177282.5 Latest version!	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu89535	XM_030242685.1 Latest version!	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu01446	NM_001193305.1 Latest version!	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu01446	XM_006507954.2 Latest version!	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu01446	XM_006507954.1	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mical2 ( NM_177282.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796256.1 Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OMu06153
Clone ID Related Accession (Same CDS sequence)	NM_177282.5
Accession Version	NM_177282.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	[F-actin]-monooxygenase MICAL2 isoform B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB662540.1, AK030608.1 and AC132392.3. On Jul 21, 2010 this sequence version replaced NM_177282.4. Transcript Variant: This variant (B) lacks 5 alternate exons, resulting in the loss of an in-frame segment in the 3' coding region, and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK143191.1, AK030608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA ATCCCCGAGA 
AAGGCATTCC CCCTGAGCCT GGGCGGCAGA GACACCTGCT ACTTTTGTAA GAAGCGTGTA 
TACATGATAG AGCGGCTGAG TGCTGAGGGC CACTTTTTCC ACCAAGAGTG CTTCCGTTGC 
AGCGTCTGCA GTGCCACCCT GCGCCTGGCT GCCTATGCCT TTGACTGCGA TGAAGGCAAA 
TTTTACTGCA AGCCCCATTT TGTTCACTGC AAAACCAGTA GCAAACAGCG AAAGAGACGG 
GCAGAGCTGA ATCAGCAAAG AGAGGAGGAA GGAACATGGC AGGAGCAGGA AGCACCTCGG 
AGGGATGTAC CCACAGAAAG CTCTTGTGCA GTGGCGGCCA TCAGCACGCC AGAAGGCAGC 
CCCCCAGGTA CCTCCACCTC CTTCTTTAGG AAGGCACTCA GCTGGCCTCT CCGGCTGACA 
AGAGGCCTGC TCAACCTGCC CCAGAGCCTG CTTAGGTGGA TGCAGGGACT CCAGGAGGCT 
GCTGGCCACC ATGTGCGGGA CAATGCTCAC AACTACTGCT TCATGTTTGA GCTCCTGAGC 
CTGGGGCTGC TACTGCTCTG GGCTTTCTCC AAGGTCCTGG CTGCCATGTA CAGAGAGTCT 
GAGGAGTCTC TGGAGAACAT CCGCAGCTGG CTGCTCAGGT TTATCCCAGT GAAGCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796256.1
CDS	336..3218
Translation	MGENEDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYSQLKSKVNTWKAKALWHKLDKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEVHVNVEFVRVLEPPEDQENQKVGWRAEFLPADHALSDFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCSENVNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTPPLEVLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNLHCVRPHQVKHLYITKEMDRFPLERWGSVRRSVSLSRRESDIRPNKLLTWCQQQTKGYQHVRVTDLTTSWRSGLALCAIIHSFRPELINFDSLNEDDAVENNQLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGTPLRPMDSWRKNYGENADFGLGKTFIQNNYLNLTLPRKRTPRVDTQTEENDMNKRRRQGFNHLEELPSFSSRSLGSSQEYAKESGSQNKVKHMANQLLAKFEENTRNPSVVKQESPRKAFPLSLGGRDTCYFCKKRVYMIERLSAEGHFFHQECFRCSVCSATLRLAAYAFDCDEGKFYCKPHFVHCKTSSKQRKRRAELNQQREEEGTWQEQEAPRRDVPTESSCAVAAISTPEGSPPGTSTSFFRKALSWPLRLTRGLLNLPQSLLRWMQGLQEAAGHHVRDNAHNYCFMFELLSLGLLLLWAFSKVLAAMYRESEESLENIRSWLLRFIPVKLQ

Target ORF information:

RefSeq Version	NM_177282.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177282.5

ORF Insert Sequence:

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA ATCCCCGAGA 
AAGGCATTCC CCCTGAGCCT GGGCGGCAGA GACACCTGCT ACTTTTGTAA GAAGCGTGTA 
TACATGATAG AGCGGCTGAG TGCTGAGGGC CACTTTTTCC ACCAAGAGTG CTTCCGTTGC 
AGCGTCTGCA GTGCCACCCT GCGCCTGGCT GCCTATGCCT TTGACTGCGA TGAAGGCAAA 
TTTTACTGCA AGCCCCATTT TGTTCACTGC AAAACCAGTA GCAAACAGCG AAAGAGACGG 
GCAGAGCTGA ATCAGCAAAG AGAGGAGGAA GGAACATGGC AGGAGCAGGA AGCACCTCGG 
AGGGATGTAC CCACAGAAAG CTCTTGTGCA GTGGCGGCCA TCAGCACGCC AGAAGGCAGC 
CCCCCAGGTA CCTCCACCTC CTTCTTTAGG AAGGCACTCA GCTGGCCTCT CCGGCTGACA 
AGAGGCCTGC TCAACCTGCC CCAGAGCCTG CTTAGGTGGA TGCAGGGACT CCAGGAGGCT 
GCTGGCCACC ATGTGCGGGA CAATGCTCAC AACTACTGCT TCATGTTTGA GCTCCTGAGC 
CTGGGGCTGC TACTGCTCTG GGCTTTCTCC AAGGTCCTGG CTGCCATGTA CAGAGAGTCT 
GAGGAGTCTC TGGAGAACAT CCGCAGCTGG CTGCTCAGGT TTATCCCAGT GAAGCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mical2 ( XM_030242685.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098545.1 Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu89535
Clone ID Related Accession (Same CDS sequence)	XM_030242685.1
Accession Version	XM_030242685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	F-actin-monooxygenase MICAL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA ATCCCCGAGA 
AAGGCATTCC CCCTGAGCCT GGGCGGCAGA GACACCTGCT ACTTTTGTAA GAAGCGTGTA 
TACATGATAG AGCGGCTGAG TGCTGAGGGC CACTTTTTCC ACCAAGAGTG CTTCCGTTGC 
AGCGTCTGCA GTGCCACCCT GCGCCTGGCT GCCTATGCCT TTGACTGCGA TGAAGGCAAA 
TTTTACTGCA AGCCCCATTT TGTTCACTGC AAAACCAGTA GCAAACAGCG AAAGAGACGG 
GCAGAGCTGA ATCAGCAAAG AGAGGAGGAA GGAACATGGC AGGAGCAGGA AGCACCTCGG 
AGGGATGTAC CCACAGAAAG CTCTTGTGCA GTGGCGGCCA TCAGCACGCC AGAAGGCAGC 
CCCCCAGTAC GTTTCAGCCT TCCAGTCCTA CACCCACTTC TTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098545.1
CDS	347..2974
Translation	MGENEDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYSQLKSKVNTWKAKALWHKLDKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEVHVNVEFVRVLEPPEDQENQKVGWRAEFLPADHALSDFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCSENVNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTPPLEVLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNLHCVRPHQVKHLYITKEMDRFPLERWGSVRRSVSLSRRESDIRPNKLLTWCQQQTKGYQHVRVTDLTTSWRSGLALCAIIHSFRPELINFDSLNEDDAVENNQLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGTPLRPMDSWRKNYGENADFGLGKTFIQNNYLNLTLPRKRTPRVDTQTEENDMNKRRRQGFNHLEELPSFSSRSLGSSQEYAKESGSQNKVKHMANQLLAKFEENTRNPSVVKQESPRKAFPLSLGGRDTCYFCKKRVYMIERLSAEGHFFHQECFRCSVCSATLRLAAYAFDCDEGKFYCKPHFVHCKTSSKQRKRRAELNQQREEEGTWQEQEAPRRDVPTESSCAVAAISTPEGSPPVRFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_030242685.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242685.1

ORF Insert Sequence:

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA ATCCCCGAGA 
AAGGCATTCC CCCTGAGCCT GGGCGGCAGA GACACCTGCT ACTTTTGTAA GAAGCGTGTA 
TACATGATAG AGCGGCTGAG TGCTGAGGGC CACTTTTTCC ACCAAGAGTG CTTCCGTTGC 
AGCGTCTGCA GTGCCACCCT GCGCCTGGCT GCCTATGCCT TTGACTGCGA TGAAGGCAAA 
TTTTACTGCA AGCCCCATTT TGTTCACTGC AAAACCAGTA GCAAACAGCG AAAGAGACGG 
GCAGAGCTGA ATCAGCAAAG AGAGGAGGAA GGAACATGGC AGGAGCAGGA AGCACCTCGG 
AGGGATGTAC CCACAGAAAG CTCTTGTGCA GTGGCGGCCA TCAGCACGCC AGAAGGCAGC 
CCCCCAGTAC GTTTCAGCCT TCCAGTCCTA CACCCACTTC TTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mical2 ( NM_001193305.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001180234.1 Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant A, mRNA.

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CloneID	OMu01446
Clone ID Related Accession (Same CDS sequence)	NM_001193305.1 , XM_006507954.2 , XM_006507954.1
Accession Version	NM_001193305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	[F-actin]-monooxygenase MICAL2 isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166834.2, AC100153.10 and AC132392.3. Transcript Variant: This variant (A) encodes the longer isoform (A). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: AK220353.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180234.1
CDS	336..3644
Translation	MGENEDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYSQLKSKVNTWKAKALWHKLDKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEVHVNVEFVRVLEPPEDQENQKVGWRAEFLPADHALSDFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCSENVNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTPPLEVLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNLHCVRPHQVKHLYITKEMDRFPLERWGSVRRSVSLSRRESDIRPNKLLTWCQQQTKGYQHVRVTDLTTSWRSGLALCAIIHSFRPELINFDSLNEDDAVENNQLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGTPLRPMDSWRKNYGENADFGLGKTFIQNNYLNLTLPRKRTPRVDTQTEENDMNKRRRQGFNHLEELPSFSSRSLGSSQEYAKESGSQNKVKHMANQLLAKFEENTRNPSVVKQDCRRVSGIGKPVLCSASRPPGTSCCPKLEESTPRLPPPLKRQFSSTVATGQVLRELNQVPASGECPSRPWRARAKSDLQLGGVENLATLPRTCQGALALSGVLRRLQQVEEKVLQKRAQNLANREFHTKNIKEKAAHLASMFGHGDLPQDKLLSKRVPHAHPPSPPSCLPSPHPAAASSPPAADSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESPRKAFPLSLGGRDTCYFCKKRVYMIERLSAEGHFFHQECFRCSVCSATLRLAAYAFDCDEGKFYCKPHFVHCKTSSKQRKRRAELNQQREEEGTWQEQEAPRRDVPTESSCAVAAISTPEGSPPVRFSLPVLHPLLG

Target ORF information:

RefSeq Version	NM_001193305.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193305.1

ORF Insert Sequence:

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mical2 ( XM_006507954.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508017.1 Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.

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CloneID	OMu01446
Clone ID Related Accession (Same CDS sequence)	NM_001193305.1 , XM_006507954.2 , XM_006507954.1
Accession Version	XM_006507954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	F-actin-monooxygenase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508017.1
CDS	347..3655
Translation	MGENEDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYSQLKSKVNTWKAKALWHKLDKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEVHVNVEFVRVLEPPEDQENQKVGWRAEFLPADHALSDFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCSENVNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTPPLEVLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNLHCVRPHQVKHLYITKEMDRFPLERWGSVRRSVSLSRRESDIRPNKLLTWCQQQTKGYQHVRVTDLTTSWRSGLALCAIIHSFRPELINFDSLNEDDAVENNQLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGTPLRPMDSWRKNYGENADFGLGKTFIQNNYLNLTLPRKRTPRVDTQTEENDMNKRRRQGFNHLEELPSFSSRSLGSSQEYAKESGSQNKVKHMANQLLAKFEENTRNPSVVKQDCRRVSGIGKPVLCSASRPPGTSCCPKLEESTPRLPPPLKRQFSSTVATGQVLRELNQVPASGECPSRPWRARAKSDLQLGGVENLATLPRTCQGALALSGVLRRLQQVEEKVLQKRAQNLANREFHTKNIKEKAAHLASMFGHGDLPQDKLLSKRVPHAHPPSPPSCLPSPHPAAASSPPAADSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESPRKAFPLSLGGRDTCYFCKKRVYMIERLSAEGHFFHQECFRCSVCSATLRLAAYAFDCDEGKFYCKPHFVHCKTSSKQRKRRAELNQQREEEGTWQEQEAPRRDVPTESSCAVAAISTPEGSPPVRFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_006507954.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507954.2

ORF Insert Sequence:

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mical2 ( XM_006507954.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508017.1 Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.

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CloneID	OMu01446
Clone ID Related Accession (Same CDS sequence)	NM_001193305.1 , XM_006507954.2 , XM_006507954.1
Accession Version	XM_006507954.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508017.1
CDS	331..3639
Translation	MGENEDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYSQLKSKVNTWKAKALWHKLDKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEVHVNVEFVRVLEPPEDQENQKVGWRAEFLPADHALSDFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCSENVNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTPPLEVLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNLHCVRPHQVKHLYITKEMDRFPLERWGSVRRSVSLSRRESDIRPNKLLTWCQQQTKGYQHVRVTDLTTSWRSGLALCAIIHSFRPELINFDSLNEDDAVENNQLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGTPLRPMDSWRKNYGENADFGLGKTFIQNNYLNLTLPRKRTPRVDTQTEENDMNKRRRQGFNHLEELPSFSSRSLGSSQEYAKESGSQNKVKHMANQLLAKFEENTRNPSVVKQDCRRVSGIGKPVLCSASRPPGTSCCPKLEESTPRLPPPLKRQFSSTVATGQVLRELNQVPASGECPSRPWRARAKSDLQLGGVENLATLPRTCQGALALSGVLRRLQQVEEKVLQKRAQNLANREFHTKNIKEKAAHLASMFGHGDLPQDKLLSKRVPHAHPPSPPSCLPSPHPAAASSPPAADSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESPRKAFPLSLGGRDTCYFCKKRVYMIERLSAEGHFFHQECFRCSVCSATLRLAAYAFDCDEGKFYCKPHFVHCKTSSKQRKRRAELNQQREEEGTWQEQEAPRRDVPTESSCAVAAISTPEGSPPVRFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_006507954.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated monooxygenase, calponin and LIM domain containing 2 (Mical2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507954.1

ORF Insert Sequence:

ATGGGAGAGA ATGAAGATGA GAAGCAGGCG CAGGCCAGCC AGGTCTTCGA GAACTTTGTG 
CAAGCTACCA CATGCAAAGG GACCCTCCAG GCCTTCAACA TCCTCACCTG CCTCCTGGAC 
CTAGATCCGC TGGACCATAG GAACTTCTAC TCCCAGCTCA AGTCCAAGGT GAACACCTGG 
AAGGCCAAAG CCCTGTGGCA CAAACTGGAT AAGCGCGGCT CCCACAAGGA GTACAAGCGA 
GGAAAAGCCT GCTCGAACAC TAAGTGTCTC ATCGTCGGAG GAGGACCATG TGGCTTGCGC 
ACTGCCATTG AACTTGCCTA CCTGGGAGCC AAAGTGGTTG TGGTGGAGAA GAGGGACACC 
TTCTCCCGGA ACAATGTCCT GCACCTCTGG CCCTTCACTA TCCATGACCT GCGGGGCCTG 
GGGGCCAAGA AGTTCTATGG GAAATTCTGT GCTGGCTCCA TCGACCACAT CAGTATCCGA 
CAACTGCAGC TTATCCTCTT CAAGGTGGCC CTGATGCTGG GAGTGGAGGT CCACGTGAAT 
GTGGAGTTTG TGAGGGTGCT GGAGCCTCCT GAAGACCAAG AGAATCAAAA AGTTGGATGG 
CGGGCAGAAT TCCTTCCTGC AGACCACGCC CTGTCTGACT TTGAGTTTGA TGTCATCATC 
GGTGCTGACG GTCACAGGAA CACGCTAGAA GGCTTCAGGA GGAAAGAGTT CCGAGGGAAG 
CTGGCCATCG CCATCACCGC CAACTTCATA AACAGGAACA GCACAGCTGA GGCCAAGGTG 
GAGGAGATCA GTGGTGTTGC CTTCATCTTC AACCAGAAGT TCTTCCAGGA CCTGAAGGAA 
GAAACAGGGA TTGATCTCGA GAACATTGTT TACTATAAGG ACAGTACCCA CTACTTTGTC 
ATGACAGCCA AGAAGCAGAG CCTGCTGGAC AAGGGCGTCA TCCTTAATGA CTACATTGAC 
ACAGAGATGC TGCTGTGTTC GGAGAATGTG AACCAGGACA ACCTGCTCTC CTACGCCAGA 
GAAGCCGCTG ACTTTGCCAC CAACTACCAG CTGCCATCCT TAGACTTTGC CATCAATCAC 
AACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACCTCCA TGTATGCCTC AGAGAACGCA 
GCTCTGATGC GTGAGCGCCA GGCACACCAG CTGCTCGTGG CTCTTGTGGG CGACAGCCTG 
CTTGAGCCAT TTTGGCCCAT GGGCACAGGC TGTGCCCGAG GCTTCCTGGC AGCCTTTGAC 
ACGGCATGGA TGGTGAAGAG CTGGGACCAG GGCACCCCTC CCCTGGAGGT ATTAGCTGAA 
AGAGAGAGTC TTTACAGGCT GTTACCTCAG ACAACCCCAG AGAACATCAA CAAAAATTTT 
GAGCAGTACA CATTGGACCC AGCCACGCGG TACCCAAACC TCAACCTGCA CTGCGTCAGG 
CCTCACCAGG TGAAGCATTT GTACATCACT AAGGAGATGG ACCGCTTCCC TCTCGAGAGA 
TGGGGCTCAG TGAGGAGATC TGTCAGCCTC TCCAGGCGGG AGTCAGACAT CCGGCCTAAC 
AAGCTTTTAA CCTGGTGCCA GCAGCAGACC AAGGGTTACC AGCACGTCAG AGTCACTGAC 
CTGACCACAT CCTGGCGCAG CGGCTTGGCC CTGTGTGCCA TCATCCACAG CTTCCGGCCA 
GAGCTGATCA ACTTTGACTC GCTGAATGAA GATGACGCTG TGGAGAACAA CCAACTGGCA 
TTTGATGTGG CCAAGCGTGA GTTTGGGATC CTGCCTGTGA CCACAGGCAA AGAGATGGCA 
TCTACCCAGG AGCCAGACAA GCTCAGCATG GTCATGTACC TCTCCAAGTT CTATGAGCTC 
TTCCGGGGCA CTCCACTGAG ACCCATGGAT TCCTGGCGTA AAAACTATGG AGAAAATGCT 
GACTTTGGCT TGGGCAAAAC ATTCATTCAG AATAACTATC TCAACCTCAC ATTGCCCAGA 
AAGAGGACCC CACGGGTAGA CACCCAGACT GAAGAGAATG ACATGAACAA GAGGCGGCGA 
CAAGGCTTCA ACCACCTGGA AGAGCTGCCA TCCTTCTCCA GCCGCAGCCT GGGCTCCAGT 
CAAGAGTATG CTAAAGAAAG TGGCAGTCAG AACAAGGTCA AGCACATGGC CAATCAGCTG 
CTAGCCAAGT TTGAGGAGAA CACTCGGAAC CCTTCAGTCG TGAAACAGGA CTGCCGCCGG 
GTCTCAGGCA TAGGTAAGCC TGTCCTGTGC TCTGCCTCTC GCCCTCCTGG CACCTCTTGC 
TGCCCCAAGC TGGAGGAGTC CACTCCCAGG CTTCCACCTC CTCTGAAAAG GCAGTTCTCC 
TCCACGGTGG CAACAGGACA GGTGCTCAGA GAACTCAACC AAGTACCGGC TAGTGGCGAG 
TGCCCCAGCA GACCCTGGAG AGCCAGGGCC AAGTCAGACC TGCAACTGGG TGGGGTTGAA 
AATCTCGCTA CCCTGCCTCG CACCTGCCAA GGGGCACTGG CCCTGTCCGG GGTGCTGCGG 
CGCCTACAGC AAGTAGAAGA AAAGGTGCTT CAGAAGAGGG CCCAGAACTT GGCTAACAGG 
GAATTTCACA CAAAGAACAT TAAAGAGAAG GCAGCTCACC TGGCCTCCAT GTTTGGACAC 
GGGGATTTAC CACAGGATAA GCTCCTATCT AAACGCGTGC CTCACGCTCA TCCTCCATCT 
CCTCCCTCTT GCCTTCCGTC TCCTCATCCA GCTGCTGCTT CCTCTCCACC CGCTGCCGAC 
TCTGTTTCTC CTGCCAGAAA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACACA GGCCACCTCT 
CCAGACCTGG AATCCCCGAG AAAGGCATTC CCCCTGAGCC TGGGCGGCAG AGACACCTGC 
TACTTTTGTA AGAAGCGTGT ATACATGATA GAGCGGCTGA GTGCTGAGGG CCACTTTTTC 
CACCAAGAGT GCTTCCGTTG CAGCGTCTGC AGTGCCACCC TGCGCCTGGC TGCCTATGCC 
TTTGACTGCG ATGAAGGCAA ATTTTACTGC AAGCCCCATT TTGTTCACTG CAAAACCAGT 
AGCAAACAGC GAAAGAGACG GGCAGAGCTG AATCAGCAAA GAGAGGAGGA AGGAACATGG 
CAGGAGCAGG AAGCACCTCG GAGGGATGTA CCCACAGAAA GCTCTTGTGC AGTGGCGGCC 
ATCAGCACGC CAGAAGGCAG CCCCCCAGTA CGTTTCAGCC TTCCAGTCCT ACACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-205-5p suppresses pulmonary vascular smooth muscle cell proliferation by targeting MICAL2-mediated Erk1/2 signaling.
Microvascular research12443-50(2019 07)
Tao W,Sun W,Zhu H,Zhang J

Actin stimulates reduction of the MICAL-2 monooxygenase domain.
Biochemistry52(35)6076-84(2013 Sep)
McDonald CA,Liu YY,Palfey BA

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Molecular cell51(3)397-404(2013 Aug)
Lee BC,Péterfi Z,Hoffmann FW,Moore RE,Kaya A,Avanesov A,Tarrago L,Zhou Y,Weerapana E,Fomenko DE,Hoffmann PR,Gladyshev VN

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map.
Proceedings of the National Academy of Sciences of the United States of America110(10)4081-6(2013 Mar)
Elsen GE,Hodge RD,Bedogni F,Daza RA,Nelson BR,Shiba N,Reiner SL,Hevner RF

MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion.
Cell109(7)887-900(2002 Jun)
Terman JR,Mao T,Pasterkamp RJ,Yu HH,Kolodkin AL

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Mical2 cDNA ORF clone, Mus musculus(house mouse)

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