Itpkb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itpkb
Entrez Gene ID	320404
Full Name	inositol 1,4,5-trisphosphate 3-kinase B
Synonyms	1110033J02Rik,E130307H12Rik
General protein information	Preferred Names inositol 1,4,5-trisphosphate 3-kinase B Names inositol-trisphosphate 3-kinase B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H4\|1 84.27 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030254970.1	XP_030110830.1	inositol-trisphosphate 3-kinase B isoform X1
NM_001081175.1	NP_001074644.1	inositol-trisphosphate 3-kinase B
XM_030254969.1	XP_030110829.1	inositol-trisphosphate 3-kinase B isoform X1
XM_006496902.4	XP_006496965.1	inositol-trisphosphate 3-kinase B isoform X2
XM_006496902.3	XP_006496965.1	inositol-trisphosphate 3-kinase B isoform X1
XM_006496903.3	XP_006496966.1	inositol-trisphosphate 3-kinase B isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	itpkb	XP_002938890.1
Mus musculus (house mouse)	Itpkb	NP_001074644.1
Homo sapiens (human)	ITPKB	NP_002212.3
Macaca mulatta (Rhesus monkey)	ITPKB	XP_001089824.2
Drosophila melanogaster (fruit fly)	IP3K2	NP_001096969.1
Canis lupus familiaris (dog)	ITPKB	XP_547504.2
Bos taurus (cattle)	ITPKB	XP_002707781.2
Danio rerio (zebrafish)	itpkb	XP_691475.2
Pan troglodytes (chimpanzee)	ITPKB	XP_001141610.1
Rattus norvegicus (Norway rat)	Itpkb	NP_062185.2
Gallus gallus (chicken)	ITPKB	XP_415039.2

Gene Ontology
Bibliography

Related articles in PubMed

Non-canonical antagonism of PI3K by the kinase Itpkb delays thymocyte β-selection and renders it Notch-dependent.
Westernberg L, Conche C, Huang YH, Rigaud S, Deng Y, Siegemund S, Mukherjee S, Nosaka L, Das J, Sauer K
eLife5(2016 Feb)

IP3 3-kinase B controls hematopoietic stem cell homeostasis and prevents lethal hematopoietic failure in mice.
Siegemund S, Rigaud S, Conche C, Broaten B, Schaffer L, Westernberg L, Head SR, Sauer K
Blood125(18)2786-97(2015 Apr)

Inhibition of the Inositol Kinase Itpkb Augments Calcium Signaling in Lymphocytes and Reveals a Novel Strategy to Treat Autoimmune Disease.
Miller AT, Dahlberg C, Sandberg ML, Wen BG, Beisner DR, Hoerter JA, Parker A, Schmedt C, Stinson M, Avis J, Cienfuegos C, McPate M, Tranter P, Gosling M, Groot-Kormelink PJ, Dawson J, Pan S, Tian SS, Seidel HM, Cooke MP
PloS one10(6)e0131071(2015)

Inositol 1,4,5-trisphosphate 3-kinase B (Itpkb) controls survival, proliferation and cytokine production in mouse peripheral T cells.
Pouillon V, Maréchal Y, Frippiat C, Erneux C, Schurmans S
Advances in biological regulation53(1)39-50(2013 Jan)

Inositol tetrakisphosphate limits NK cell effector functions by controlling PI3K signaling.
Sauer K, Park E, Siegemund S, French AR, Wahle JA, Sternberg L, Rigaud S, Jonsson AH, Yokoyama WM, Huang YH
Blood121(2)286-97(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Itpkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itpkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28793	XM_006496903.3 Latest version!	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu01815	NM_001081175.1 Latest version!	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu01815	XM_030254970.1 Latest version!	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu01815	XM_030254969.1 Latest version!	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu28792	XM_006496902.4 Latest version!	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu28792	XM_006496902.3	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itpkb ( XM_006496903.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496966.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28793
Clone ID Related Accession (Same CDS sequence)	XM_006496903.3
Accession Version	XM_006496903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACCCGAGAG AACTCGGCAG GACTGGGAAA TGAGCAAGTC ATGGAGAAAG 
ATAAAGAACA TGGTGCAGTG GTCCCCCTTT GTCATGTCCT TCAAGAAGAA GTACCCCTGG 
ATCCAGCTGG CAGGACATGC AGGGAGCTTC AAGGCAGCTG CAAATGGCCG CATCCTTAAG 
AAGCACTGTG AGTCTGAGCA GCGCTGCCTG GACCGGTTAA TGGCGGATGT GCTGAGACCC 
TTCGTGCCAG CCTACCATGG GGACGTGGTG AAGGATGGGG AACGCTACAA CCAGATGGAC 
GACCTGCTGG CTGACTTCGA TTCGCCCTGC GTGATGGACT GCAAGATGGG TGTCAGGACA 
TACCTGGAGG AAGAACTCAC CAAGGCCCGG AAGAAGCCTA GCTTGCGGAA GGACATGTAC 
CAGAAGATGG TCGAGGTGGA CCCTGAGGCC CCCACTGAAG AGGAGAAAGC CCAGCGAGCT 
GTGACCAAGC CACGTTACAT GCAGTGGCGG GAAACCATCA GTTCGACAGC CACCCTAGGC 
TTCAGGATCG AAGGCATCAA GAAGGAAGAT GGCTCTGTGA ACCGTGACTT CAAGAAGACC 
AAAACAAGGG AGCAGGTCAC CGAGGCTTTC AGAGAATTCA CTAAGGGAAA CCAGAACATC 
TTGATCGCCT ACCGGGACCG GCTGAAGGCC ATTCGAGCGA CCCTGGAAAT CTCTCCCTTC 
TTCAAGTGCC ACGAGGTCAT TGGCAGCTCT CTCCTCTTCA TCCATGACAA GAAGGAGCAA 
GCCAAGGTGT GGATGATTGA CTTTGGGAAA ACCACGCCCC TTCCGGAAGG CCAGACCCTA 
CAACACGACG TCCCCTGGCA GGAGGGGAAC CGGGAGGATG GCTACCTCTC AGGGCTGGAC 
AACCTCATCG ACATCCTGAC GGAAATGTCC CAGGGCAGCC CACTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496966.1
CDS	306..1256
Translation	MARRPERTRQDWEMSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLDNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	XM_006496903.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496903.3

ORF Insert Sequence:

ATGGCCAGAA GACCCGAGAG AACTCGGCAG GACTGGGAAA TGAGCAAGTC ATGGAGAAAG 
ATAAAGAACA TGGTGCAGTG GTCCCCCTTT GTCATGTCCT TCAAGAAGAA GTACCCCTGG 
ATCCAGCTGG CAGGACATGC AGGGAGCTTC AAGGCAGCTG CAAATGGCCG CATCCTTAAG 
AAGCACTGTG AGTCTGAGCA GCGCTGCCTG GACCGGTTAA TGGCGGATGT GCTGAGACCC 
TTCGTGCCAG CCTACCATGG GGACGTGGTG AAGGATGGGG AACGCTACAA CCAGATGGAC 
GACCTGCTGG CTGACTTCGA TTCGCCCTGC GTGATGGACT GCAAGATGGG TGTCAGGACA 
TACCTGGAGG AAGAACTCAC CAAGGCCCGG AAGAAGCCTA GCTTGCGGAA GGACATGTAC 
CAGAAGATGG TCGAGGTGGA CCCTGAGGCC CCCACTGAAG AGGAGAAAGC CCAGCGAGCT 
GTGACCAAGC CACGTTACAT GCAGTGGCGG GAAACCATCA GTTCGACAGC CACCCTAGGC 
TTCAGGATCG AAGGCATCAA GAAGGAAGAT GGCTCTGTGA ACCGTGACTT CAAGAAGACC 
AAAACAAGGG AGCAGGTCAC CGAGGCTTTC AGAGAATTCA CTAAGGGAAA CCAGAACATC 
TTGATCGCCT ACCGGGACCG GCTGAAGGCC ATTCGAGCGA CCCTGGAAAT CTCTCCCTTC 
TTCAAGTGCC ACGAGGTCAT TGGCAGCTCT CTCCTCTTCA TCCATGACAA GAAGGAGCAA 
GCCAAGGTGT GGATGATTGA CTTTGGGAAA ACCACGCCCC TTCCGGAAGG CCAGACCCTA 
CAACACGACG TCCCCTGGCA GGAGGGGAAC CGGGAGGATG GCTACCTCTC AGGGCTGGAC 
AACCTCATCG ACATCCTGAC GGAAATGTCC CAGGGCAGCC CACTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( NM_001081175.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074644.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), mRNA.

Price & Availability↑

CloneID	OMu01815
Clone ID Related Accession (Same CDS sequence)	XM_030254970.1 , NM_001081175.1 , XM_030254969.1
Accession Version	NM_001081175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527177600000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC121838.2. On Feb 6, 2007 this sequence version replaced XM_205854.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC157986.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074644.1
CDS	504..3332
Translation	MAVYCYALNSLVIMNSTNELKSGGPRPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSPGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVAARILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDTNSGRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGTPASPRCGSPTPMETDKRVAPSLERFGTSLTLATKVAASAASAGPHPGHDSALMETGCELGGMRPWEAQMERRGQFLGKETGSTPEPVRTHMREPPGRVGRGIHSVGGQGSWTPEVIKRPEERAVTAQSSEPSEDPRWSRLPVDLDSVGPEKGGNRIPGMRGPQQTLDSEREGSPALGLLGGSQAAQPGARGVEEDVHYGRMLEPLPPGEVTTKLKEPQCLPGDRMGMQPESSIVWPSALEEAPLIWTRDTGVQSKGTWGSQLPDGDAHPSCQEMPPDQKDKASLKEACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYDDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLDNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	NM_001081175.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081175.1

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_030254970.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110830.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01815
Clone ID Related Accession (Same CDS sequence)	XM_030254970.1 , NM_001081175.1 , XM_030254969.1
Accession Version	XM_030254970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110830.1
CDS	240..3068
Translation	MAVYCYALNSLVIMNSTNELKSGGPRPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSPGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVAARILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDTNSGRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGTPASPRCGSPTPMETDKRVAPSLERFGTSLTLATKVAASAASAGPHPGHDSALMETGCELGGMRPWEAQMERRGQFLGKETGSTPEPVRTHMREPPGRVGRGIHSVGGQGSWTPEVIKRPEERAVTAQSSEPSEDPRWSRLPVDLDSVGPEKGGNRIPGMRGPQQTLDSEREGSPALGLLGGSQAAQPGARGVEEDVHYGRMLEPLPPGEVTTKLKEPQCLPGDRMGMQPESSIVWPSALEEAPLIWTRDTGVQSKGTWGSQLPDGDAHPSCQEMPPDQKDKASLKEACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYDDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLDNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	XM_030254970.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254970.1

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_030254969.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110829.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

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CloneID	OMu01815
Clone ID Related Accession (Same CDS sequence)	XM_030254970.1 , NM_001081175.1 , XM_030254969.1
Accession Version	XM_030254969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110829.1
CDS	270..3098
Translation	MAVYCYALNSLVIMNSTNELKSGGPRPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSPGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVAARILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDTNSGRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGTPASPRCGSPTPMETDKRVAPSLERFGTSLTLATKVAASAASAGPHPGHDSALMETGCELGGMRPWEAQMERRGQFLGKETGSTPEPVRTHMREPPGRVGRGIHSVGGQGSWTPEVIKRPEERAVTAQSSEPSEDPRWSRLPVDLDSVGPEKGGNRIPGMRGPQQTLDSEREGSPALGLLGGSQAAQPGARGVEEDVHYGRMLEPLPPGEVTTKLKEPQCLPGDRMGMQPESSIVWPSALEEAPLIWTRDTGVQSKGTWGSQLPDGDAHPSCQEMPPDQKDKASLKEACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYDDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLDNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	XM_030254969.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254969.1

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
AGCAAGTCAT GGAGAAAGAT AAAGAACATG GTGCAGTGGT CCCCCTTTGT CATGTCCTTC 
AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACATGCAG GGAGCTTCAA GGCAGCTGCA 
AATGGCCGCA TCCTTAAGAA GCACTGTGAG TCTGAGCAGC GCTGCCTGGA CCGGTTAATG 
GCGGATGTGC TGAGACCCTT CGTGCCAGCC TACCATGGGG ACGTGGTGAA GGATGGGGAA 
CGCTACAACC AGATGGACGA CCTGCTGGCT GACTTCGATT CGCCCTGCGT GATGGACTGC 
AAGATGGGTG TCAGGACATA CCTGGAGGAA GAACTCACCA AGGCCCGGAA GAAGCCTAGC 
TTGCGGAAGG ACATGTACCA GAAGATGGTC GAGGTGGACC CTGAGGCCCC CACTGAAGAG 
GAGAAAGCCC AGCGAGCTGT GACCAAGCCA CGTTACATGC AGTGGCGGGA AACCATCAGT 
TCGACAGCCA CCCTAGGCTT CAGGATCGAA GGCATCAAGA AGGAAGATGG CTCTGTGAAC 
CGTGACTTCA AGAAGACCAA AACAAGGGAG CAGGTCACCG AGGCTTTCAG AGAATTCACT 
AAGGGAAACC AGAACATCTT GATCGCCTAC CGGGACCGGC TGAAGGCCAT TCGAGCGACC 
CTGGAAATCT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG GCAGCTCTCT CCTCTTCATC 
CATGACAAGA AGGAGCAAGC CAAGGTGTGG ATGATTGACT TTGGGAAAAC CACGCCCCTT 
CCGGAAGGCC AGACCCTACA ACACGACGTC CCCTGGCAGG AGGGGAACCG GGAGGATGGC 
TACCTCTCAG GGCTGGACAA CCTCATCGAC ATCCTGACGG AAATGTCCCA GGGCAGCCCA 
CTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_006496902.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496965.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X3, mRNA.

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CloneID	OMu28792
Clone ID Related Accession (Same CDS sequence)	XM_006496902.4 , XM_006496902.3
Accession Version	XM_006496902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496902.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
GTGCAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496965.1
CDS	590..2521
Translation	MAVYCYALNSLVIMNSTNELKSGGPRPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSPGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVAARILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDTNSGRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGTPASPRCGSPTPMETDKRVAPSLERFGTSLTLATKVAASAASAGPHPGHDSALMETGCELGGMRPWEAQMERRGQFLGKETGSTPEPVRTHMREPPGRVGRGIHSVGGQGSWTPEVIKRPEERAVTAQSSEPSEDPRWSRLPVDLDSVGPEKGGNRIPGMRGPQQTLDSEREGSPALGLLGGSQAAQPGARGVEEDVHYGRMLEPLPPGEVTTKLKEPQCLPGDRMGMQPESSIVWPSALEEAPLIWTRDTGVQSKGTWGSQLPDGDAHPSCQEMPPDQKDKASLKEACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYDDSEEDISSDPERTLDPNSAFLHTLDQQKPRVVQV

Target ORF information:

RefSeq Version	XM_006496902.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496902.4

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
GTGCAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_006496902.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496965.1 Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

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CloneID	OMu28792
Clone ID Related Accession (Same CDS sequence)	XM_006496902.4 , XM_006496902.3
Accession Version	XM_006496902.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	inositol-trisphosphate 3-kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
GTGCAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496965.1
CDS	498..2429
Translation	MAVYCYALNSLVIMNSTNELKSGGPRPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSPGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVAARILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDTNSGRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGTPASPRCGSPTPMETDKRVAPSLERFGTSLTLATKVAASAASAGPHPGHDSALMETGCELGGMRPWEAQMERRGQFLGKETGSTPEPVRTHMREPPGRVGRGIHSVGGQGSWTPEVIKRPEERAVTAQSSEPSEDPRWSRLPVDLDSVGPEKGGNRIPGMRGPQQTLDSEREGSPALGLLGGSQAAQPGARGVEEDVHYGRMLEPLPPGEVTTKLKEPQCLPGDRMGMQPESSIVWPSALEEAPLIWTRDTGVQSKGTWGSQLPDGDAHPSCQEMPPDQKDKASLKEACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYDDSEEDISSDPERTLDPNSAFLHTLDQQKPRVVQV

Target ORF information:

RefSeq Version	XM_006496902.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus inositol 1,4,5-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496902.3

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTC 
AAGAGTGGCG GCCCCCGGCC CAGCGGCAGC GAGACGCCCC AGCCCTCCGG GAGGGCCGCG 
CTGAGTCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCTT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CCCTGGAGGA GCCCGGGAGT CCTGGGGGCT GGCGCAGCGG CCGGCGCAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
GCCCGCATCC TCTCCCCACC TGGGCCAGAG GAGGCCCAGA GGAAGCTGCG GATTCTGCAG 
CGCGAGTTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGAGCT CTGCCATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG TAGCAGCCAG CCCCCCGAAA GGGTCTTGGC TCCGTGTTCT 
CCAAGTGAGG AACGCAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAAAC CCCAGATACC 
AACTCTGGAA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCCGTG 
AGAATGGAAA GAGGTACCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAACT 
GACAAGAGGG TTGCTCCCTC ACTGGAGCGC TTTGGAACTA GCTTAACGTT GGCTACTAAA 
GTGGCAGCTT CAGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGC CCTCATGGAG 
ACAGGCTGTG AGCTCGGGGG CATGCGCCCC TGGGAGGCCC AGATGGAGAG ACGGGGGCAG 
TTTCTGGGCA AGGAGACCGG CTCAACCCCA GAGCCTGTCC GGACCCACAT GAGAGAACCC 
CCTGGAAGGG TGGGAAGAGG AATTCATTCT GTTGGTGGGC AGGGCTCCTG GACACCTGAA 
GTCATCAAAA GACCAGAAGA GAGGGCTGTG ACTGCCCAAA GCTCAGAGCC CTCAGAGGAC 
CCCAGATGGT CTAGACTGCC TGTAGACCTG GATTCCGTAG GACCTGAGAA GGGAGGAAAT 
AGGATCCCAG GGATGCGAGG GCCCCAGCAG ACCCTGGACA GTGAGAGAGA AGGCTCTCCA 
GCACTGGGCT TGCTTGGGGG CAGCCAGGCA GCACAGCCAG GGGCTAGGGG TGTGGAGGAA 
GACGTTCATT ATGGCCGAAT GCTGGAACCT TTACCACCTG GGGAAGTAAC AACAAAATTG 
AAAGAACCCC AGTGCCTCCC TGGGGACAGG ATGGGAATGC AGCCTGAGAG TTCCATAGTT 
TGGCCCAGTG CCTTGGAGGA AGCTCCCCTG ATCTGGACAC GTGACACGGG GGTACAGTCG 
AAGGGGACTT GGGGAAGCCA GCTGCCAGAC GGAGATGCTC ACCCTAGCTG CCAAGAGATG 
CCCCCAGACC AGAAGGACAA GGCCTCCTTA AAAGAGGCCT GCAGCCCCAG CAACATCCCA 
GCCATCCCTG CAGTCATCAT TACAGATATG GGTGCTCAGG AGGATGGAGG GCTAGAGGAG 
ATCCAAGGAA GCCCTCGGGG TCCCCTGCCT CTGAGGAAGC TGTCCTCCTC CTCAGCCTCC 
TCCACTGGCT TCTCCTCTTC TTACGATGAC TCGGAGGAGG ACATCTCCAG TGACCCTGAG 
CGCACGCTGG ACCCCAACTC AGCCTTTTTG CATACCCTGG ACCAGCAGAA GCCCAGAGTG 
GTGCAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-canonical antagonism of PI3K by the kinase Itpkb delays thymocyte β-selection and renders it Notch-dependent.
eLife5(2016 Feb)
Westernberg L,Conche C,Huang YH,Rigaud S,Deng Y,Siegemund S,Mukherjee S,Nosaka L,Das J,Sauer K

IP3 3-kinase B controls hematopoietic stem cell homeostasis and prevents lethal hematopoietic failure in mice.
Blood125(18)2786-97(2015 Apr)
Siegemund S,Rigaud S,Conche C,Broaten B,Schaffer L,Westernberg L,Head SR,Sauer K

Inhibition of the Inositol Kinase Itpkb Augments Calcium Signaling in Lymphocytes and Reveals a Novel Strategy to Treat Autoimmune Disease.
PloS one10(6)e0131071(2015)
Miller AT,Dahlberg C,Sandberg ML,Wen BG,Beisner DR,Hoerter JA,Parker A,Schmedt C,Stinson M,Avis J,Cienfuegos C,McPate M,Tranter P,Gosling M,Groot-Kormelink PJ,Dawson J,Pan S,Tian SS,Seidel HM,Cooke MP

Inositol 1,4,5-trisphosphate 3-kinase B (Itpkb) controls survival, proliferation and cytokine production in mouse peripheral T cells.
Advances in biological regulation53(1)39-50(2013 Jan)
Pouillon V,Maréchal Y,Frippiat C,Erneux C,Schurmans S

Inositol tetrakisphosphate limits NK cell effector functions by controlling PI3K signaling.
Blood121(2)286-97(2013 Jan)
Sauer K,Park E,Siegemund S,French AR,Wahle JA,Sternberg L,Rigaud S,Jonsson AH,Yokoyama WM,Huang YH

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Itpkb cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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