Mettl20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mettl20
Entrez Gene ID	320204
Full Name	electron transfer flavoprotein beta subunit lysine methyltransferase
Synonyms	4833442J19Rik,AI256744,ETFB-KMT,Mettl20
General protein information	Preferred Names electron transfer flavoprotein beta subunit lysine methyltransferase Names electron transfer flavoprotein beta subunit lysine methyltransferase ETFB lysine methyltransferase methyltransferase like 20 methyltransferase-like protein 20 protein N-lysine methyltransferase METTL20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_177101.5	NP_796075.2	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
NM_001252097.1	NP_001239026.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform b
XM_030255456.1	XP_030111316.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X2
XM_017321637.2	XP_017177126.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X1
NM_001252094.1	NP_001239023.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
NM_001252095.1	NP_001239024.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
NM_001252096.1	NP_001239025.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
XM_017321637.1	XP_017177126.1	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mettl20	NP_001239025.1
Xenopus tropicalis (tropical clawed frog)	LOC100486545	XP_002941663.1
Homo sapiens (human)	METTL20	NP_776163.1
Pan troglodytes (chimpanzee)	METTL20	XP_001136932.1
Danio rerio (zebrafish)	mettl20	NP_001154967.1
Macaca mulatta (Rhesus monkey)	METTL20	XP_001084019.1
Canis lupus familiaris (dog)	METTL20	XP_005637060.1
Bos taurus (cattle)	METTL20	XP_005206951.1
Rattus norvegicus (Norway rat)	Mettl20	NP_942067.1
Gallus gallus (chicken)	METTL20	XP_416369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007652	XP_308218.4
Caenorhabditis elegans (roundworm)	C37A2.6	NP_491943.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions.
Shimazu T, Furuse T, Balan S, Yamada I, Okuno S, Iwanari H, Suzuki T, Hamakubo T, Dohmae N, Yoshikawa T, Wakana S, Shinkai Y
Scientific reports8(1)1179(2018 01)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Human METTL20 methylates lysine residues adjacent to the recognition loop of the electron transfer flavoprotein in mitochondria.
Rhein VF, Carroll J, He J, Ding S, Fearnley IM, Walker JE
The Journal of biological chemistry289(35)24640-51(2014 Aug)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mettl20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mettl20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14083	NM_001252097.1 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu14083	XM_030255456.1 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu15581	NM_001252096.1 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OMu15581	NM_177101.5 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OMu15581	NM_001252094.1 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OMu15581	NM_001252095.1 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OMu15581	XM_017321637.2 Latest version!	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
View Old Accession Versions >>
OMu15581	XM_017321637.1	Mus musculus methyltransferase like 20 (Mettl20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mettl20 ( NM_001252097.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239026.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu14083
Clone ID Related Accession (Same CDS sequence)	NM_001252097.1 , XM_030255456.1
Accession Version	NM_001252097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY081844.1 and BC046911.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BC046911.1, BU503956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGAG CATCAAAGAT CTTGGCCAAC GACATAGACC CCATTGCAGG AATGGCGATT 
ACACTAAATT GCAAATTAAA TGGACTGAAT CCTTTTCCTG TTTTAACTAA AAACATTTTG 
AATACCCAAC AAGGTAAGTT TGATCTCATT GTTCTTGGAG ATATGTTTTA TGATGAAGAC 
CTTGCAGACA GTCTTCATCT GTGGCTGCAG AACTACTTTT GGACCCATGG AACCCGAGTA 
CTGATTGGTG ACCCTGGGAG GCCCCAGTTC AGTGGACATA GCATTCGGCA TCAACTGTAC 
CAACTAGTAG AATACACACT TCCAGAGCCA ACTCAGCAAG AGAACAATGG ACTGACCACA 
AGTGCTGTAT GGGATTTTCA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239026.1
CDS	281..667
Translation	MSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	NM_001252097.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252097.1

ORF Insert Sequence:

ATGAGTGGAG CATCAAAGAT CTTGGCCAAC GACATAGACC CCATTGCAGG AATGGCGATT 
ACACTAAATT GCAAATTAAA TGGACTGAAT CCTTTTCCTG TTTTAACTAA AAACATTTTG 
AATACCCAAC AAGGTAAGTT TGATCTCATT GTTCTTGGAG ATATGTTTTA TGATGAAGAC 
CTTGCAGACA GTCTTCATCT GTGGCTGCAG AACTACTTTT GGACCCATGG AACCCGAGTA 
CTGATTGGTG ACCCTGGGAG GCCCCAGTTC AGTGGACATA GCATTCGGCA TCAACTGTAC 
CAACTAGTAG AATACACACT TCCAGAGCCA ACTCAGCAAG AGAACAATGG ACTGACCACA 
AGTGCTGTAT GGGATTTTCA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( XM_030255456.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111316.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu14083
Clone ID Related Accession (Same CDS sequence)	NM_001252097.1 , XM_030255456.1
Accession Version	XM_030255456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAG CATCAAAGAT CTTGGCCAAC GACATAGACC CCATTGCAGG AATGGCGATT 
ACACTAAATT GCAAATTAAA TGGACTGAAT CCTTTTCCTG TTTTAACTAA AAACATTTTG 
AATACCCAAC AAGGTAAGTT TGATCTCATT GTTCTTGGAG ATATGTTTTA TGATGAAGAC 
CTTGCAGACA GTCTTCATCT GTGGCTGCAG AACTACTTTT GGACCCATGG AACCCGAGTA 
CTGATTGGTG ACCCTGGGAG GCCCCAGTTC AGTGGACATA GCATTCGGCA TCAACTGTAC 
CAACTAGTAG AATACACACT TCCAGAGCCA ACTCAGCAAG AGAACAATGG ACTGACCACA 
AGTGCTGTAT GGGATTTTCA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111316.1
CDS	3442..3828
Translation	MSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	XM_030255456.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255456.1

ORF Insert Sequence:

ATGAGTGGAG CATCAAAGAT CTTGGCCAAC GACATAGACC CCATTGCAGG AATGGCGATT 
ACACTAAATT GCAAATTAAA TGGACTGAAT CCTTTTCCTG TTTTAACTAA AAACATTTTG 
AATACCCAAC AAGGTAAGTT TGATCTCATT GTTCTTGGAG ATATGTTTTA TGATGAAGAC 
CTTGCAGACA GTCTTCATCT GTGGCTGCAG AACTACTTTT GGACCCATGG AACCCGAGTA 
CTGATTGGTG ACCCTGGGAG GCCCCAGTTC AGTGGACATA GCATTCGGCA TCAACTGTAC 
CAACTAGTAG AATACACACT TCCAGAGCCA ACTCAGCAAG AGAACAATGG ACTGACCACA 
AGTGCTGTAT GGGATTTTCA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( NM_177101.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796075.2 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	NM_177101.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY081844.1 and BC048711.1. On Nov 3, 2011 this sequence version replaced NM_177101.4. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1, and encodes the longer isoform (a). Variants 1-4 all encode isoform a. ##Evidence-Data-START## Transcript exon combination :: BC065807.1, BE371351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796075.2
CDS	309..1076
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	NM_177101.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177101.5

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( NM_001252094.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239023.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	NM_001252094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY081844.1, BB614316.1, AK132096.1 and BC048711.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1-4 all encode isoform a. ##Evidence-Data-START## Transcript exon combination :: AK132096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239023.1
CDS	438..1205
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	NM_001252094.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252094.1

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( NM_001252095.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239024.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	NM_001252095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY081844.1, BY135669.1 and BC048711.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1, and encodes the longer isoform (a). Variants 1-4 all encode isoform a. ##Evidence-Data-START## Transcript exon combination :: CA460983.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239024.1
CDS	419..1186
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	NM_001252095.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252095.1

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( NM_001252096.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239025.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	NM_001252096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY081844.1, BB614316.1, DN174886.1 and BC048711.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1, and encodes the longer isoform (a). Variants 1-4 all encode isoform a. ##Evidence-Data-START## Transcript exon combination :: DN174886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239025.1
CDS	328..1095
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	NM_001252096.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252096.1

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( XM_017321637.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177126.1 Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	XM_017321637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177126.1
CDS	338..1105
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	XM_017321637.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus electron transfer flavoprotein beta subunit lysine methyltransferase (Etfbkmt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321637.2

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mettl20 ( XM_017321637.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177126.1 Mus musculus methyltransferase like 20 (Mettl20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15581
Clone ID Related Accession (Same CDS sequence)	NM_177101.5 , XM_017321637.2 , NM_001252094.1 , NM_001252095.1 , NM_001252096.1 , XM_017321637.1
Accession Version	XM_017321637.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	electron transfer flavoprotein beta subunit lysine methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177126.1
CDS	342..1109
Translation	MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP

Target ORF information:

RefSeq Version	XM_017321637.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methyltransferase like 20 (Mettl20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321637.1

ORF Insert Sequence:

ATGGCTTTCA GCCTGTGTTG GAAAGCTCCC AGGAGCCCAT GGAGTTTCCT GCAGGCTGTG 
AACAATGGGT CTCCCCTGTT TCTCTGGAGG ACAGTGGGGA GCTGTTTGGA TCCTAAGATG 
AAAGCCTACC TGGAAGAGAA CACTGAAGTC ACCAGCAGTG GTAGCCTTAC CCCGGAAATC 
CAGTTACGGC TTTTAACCCC CAGGTGCAAG TTTTGGTGGG AAAGGGCTGA CCTGTGGCCC 
TACAGTGATC CCTACTGGGC TATTTACTGG CCAGGAGGCC AGGCTCTGTC TAGGTATCTT 
TTGGATAACC CTGCTGTTGT TAGAGGAAAG TCTGTCTTAG ATCTTGGGAG TGGATGTGGA 
GCTACAGCCA TTGCTGCCAA GATGAGTGGA GCATCAAAGA TCTTGGCCAA CGACATAGAC 
CCCATTGCAG GAATGGCGAT TACACTAAAT TGCAAATTAA ATGGACTGAA TCCTTTTCCT 
GTTTTAACTA AAAACATTTT GAATACCCAA CAAGGTAAGT TTGATCTCAT TGTTCTTGGA 
GATATGTTTT ATGATGAAGA CCTTGCAGAC AGTCTTCATC TGTGGCTGCA GAACTACTTT 
TGGACCCATG GAACCCGAGT ACTGATTGGT GACCCTGGGA GGCCCCAGTT CAGTGGACAT 
AGCATTCGGC ATCAACTGTA CCAACTAGTA GAATACACAC TTCCAGAGCC AACTCAGCAA 
GAGAACAATG GACTGACCAC AAGTGCTGTA TGGGATTTTC ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions.
Scientific reports8(1)1179(2018 01)
Shimazu T,Furuse T,Balan S,Yamada I,Okuno S,Iwanari H,Suzuki T,Hamakubo T,Dohmae N,Yoshikawa T,Wakana S,Shinkai Y

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Human METTL20 methylates lysine residues adjacent to the recognition loop of the electron transfer flavoprotein in mitochondria.
The Journal of biological chemistry289(35)24640-51(2014 Aug)
Rhein VF,Carroll J,He J,Ding S,Fearnley IM,Walker JE

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Mettl20 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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