Trpa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpa1
Entrez Gene ID	277328
Full Name	transient receptor potential cation channel, subfamily A, member 1
Synonyms	Anktm1,TRPA1b
General protein information	Preferred Names transient receptor potential cation channel, subfamily A, member 1 Names transient receptor potential cation channel subfamily A member 1 ankyrin-like with transmembrane domains protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495526.2	XP_006495589.1	transient receptor potential cation channel subfamily A member 1 isoform X1
NM_001348288.1	NP_001335217.1	transient receptor potential cation channel subfamily A member 1 isoform 2
NM_177781.4	NP_808449.1	transient receptor potential cation channel subfamily A member 1
NM_177781.5	NP_808449.1	transient receptor potential cation channel subfamily A member 1 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRPA1	XP_002692772.2
Canis lupus familiaris (dog)	TRPA1	XP_544123.2
Caenorhabditis elegans (roundworm)	trpa-1	NP_502249.3
Pan troglodytes (chimpanzee)	TRPA1	XP_519806.2
Mus musculus (house mouse)	Trpa1	NP_808449.1
Danio rerio (zebrafish)	trpa1a	NP_001007066.1
Danio rerio (zebrafish)	trpa1b	NP_001007067.1
Drosophila melanogaster (fruit fly)	TrpA1	NP_648263.5
Homo sapiens (human)	TRPA1	NP_015628.2
Macaca mulatta (Rhesus monkey)	TRPA1	XP_001083172.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004863	XP_314325.4
Rattus norvegicus (Norway rat)	Trpa1	NP_997491.1
Gallus gallus (chicken)	TRPA1	XP_418294.4
Xenopus tropicalis (tropical clawed frog)	trpa1	NP_001121434.1

Gene Ontology
Bibliography

Related articles in PubMed

Antimycin A-induced mitochondrial dysfunction activates vagal sensory neurons via ROS-dependent activation of TRPA1 and ROS-independent activation of TRPV1.
Stanford KR, Hadley SH, Barannikov I, Ajmo JM, Bahia PK, Taylor-Clark TE
Brain research171594-105(2019 Jul)

Methylglyoxal and a spinal TRPA1-AC1-Epac cascade facilitate pain in the db/db mouse model of type 2 diabetes.
Griggs RB, Santos DF, Laird DE, Doolen S, Donahue RR, Wessel CR, Fu W, Sinha GP, Wang P, Zhou J, Brings S, Fleming T, Nawroth PP, Susuki K, Taylor BK
Neurobiology of disease12776-86(2019 07)

Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc: Association with Inflammation and Matrix Remodeling.
Kameda T, Zvick J, Vuk M, Sadowska A, Tam WK, Leung VY, Bölcskei K, Helyes Z, Applegate LA, Hausmann ON, Klasen J, Krupkova O, Wuertz-Kozak K
International journal of molecular sciences20(7)(2019 Apr)

TRPA1 channel contributes to myocardial ischemia-reperfusion injury.
Conklin DJ, Guo Y, Nystoriak MA, Jagatheesan G, Obal D, Kilfoil PJ, Hoetker JD, Guo L, Bolli R, Bhatnagar A
American journal of physiology. Heart and circulatory physiology316(4)H889-H899(2019 04)

Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine Models.
Moilanen LJ, Hämäläinen M, Ilmarinen P, Kankaanranta H, Nieminen RM, Moilanen E, Lehtimäki L
International archives of allergy and immunology178(3)238-247(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trpa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27151	NM_001348288.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu27151	XM_006495526.2 Latest version!	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu22951	NM_177781.5 Latest version!	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$664.30 $949.00
View Old Accession Versions >>
OMu22951	NM_177781.4	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$474.50-$664.30 $949.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpa1 ( NM_177781.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808449.1 Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), mRNA.

Price & Availability↑

CloneID	OMu22951
Clone ID Related Accession (Same CDS sequence)	NM_177781.4 , NM_177781.5
Accession Version	NM_177781.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	transient receptor potential cation channel subfamily A member 1
Comment

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAATTCA TTTCCAATAA AAATATGTAT GATTCTAGTT 
TTTTTATCAA GTATATTTGG ATATTGCAAA GAAGTGATCC AAATTTTCCA ACAGAAAAGG 
AATTACTTCC TGGATTACAA CAATGCTCTG GAATGGGTTA TCTATACAAC TAGTATCATC 
TTCGTGTTGC CCTTGTTCCT CAACATCCCA GCGTATATGC AGTGGCAATG TGGAGCAATA 
GCGATATTCT TCTACTGGAT GAACTTCCTA CTGTATCTTC AAAGGTTTGA GAACTGTGGA 
ATTTTCATTG TTATGTTGGA GGTGATTTTT AAAACATTGC TGAGATCGAC CGGAGTGTTT 
ATCTTCCTCC TACTGGCTTT TGGCCTCAGC TTTTATGTTC TCCTGAATTT CCAAGATGCC 
TTCAGCACCC CATTGCTTTC CTTAATCCAG ACATTCAGTA TGATGCTAGG AGACATCAAT 
TATCGAGATG CCTTCCTAGA ACCATTGTTT AGAAATGAGT TGGCATACCC AGTCCTGACC 
TTTGGGCAGC TTATTGCCTT CACAATGTTT GTCCCAATTG TTCTCATGAA CTTACTGATT 
GGCTTGGCGG TTGGGGACAT TGCTGAGGTC CAGAAGCATG CGTCATTGAA GAGGATTGCT 
ATGCAGGTGG AACTTCATAC CAACTTAGAA AAAAAGCTGC CACTCTGGTA CTTACGCAAA 
GTGGATCAGA GGTCCACCAT CGTGTATCCA AATAGACCCA GGCACGGCAG GATGCTACGG 
TTTTTTCATT ACTTTCTTAA TATGCAAGAA ACACGACAAG AAGTACCAAA CATTGACACA 
TGCTTGGAAA TGGAAATATT GAAACAGAAA TATCGGCTGA AGGACCTCAC TTCCCTCTTG 
GAAAAGCAGC ATGAGCTCAT CAAACTCATC ATCCAGAAGA TGGAGATCAT CTCAGAGACA 
GAAGATGAAG ATAACCATTG CTCTTTCCAA GACAGGTTCA AGAAGGAGAG GCTGGAACAG 
ATGCACAGCA AGTGGAATTT TGTCTTAAAC GCAGTTAAGA CTAAAACACA TTGTTCTATT 
AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_808449.1

Translation

MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLNSFPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQKYRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSISHPDF

Target ORF information:

RefSeq Version	NM_177781.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177781.4

ORF Insert Sequence:

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAATTCA TTTCCAATAA AAATATGTAT GATTCTAGTT 
TTTTTATCAA GTATATTTGG ATATTGCAAA GAAGTGATCC AAATTTTCCA ACAGAAAAGG 
AATTACTTCC TGGATTACAA CAATGCTCTG GAATGGGTTA TCTATACAAC TAGTATCATC 
TTCGTGTTGC CCTTGTTCCT CAACATCCCA GCGTATATGC AGTGGCAATG TGGAGCAATA 
GCGATATTCT TCTACTGGAT GAACTTCCTA CTGTATCTTC AAAGGTTTGA GAACTGTGGA 
ATTTTCATTG TTATGTTGGA GGTGATTTTT AAAACATTGC TGAGATCGAC CGGAGTGTTT 
ATCTTCCTCC TACTGGCTTT TGGCCTCAGC TTTTATGTTC TCCTGAATTT CCAAGATGCC 
TTCAGCACCC CATTGCTTTC CTTAATCCAG ACATTCAGTA TGATGCTAGG AGACATCAAT 
TATCGAGATG CCTTCCTAGA ACCATTGTTT AGAAATGAGT TGGCATACCC AGTCCTGACC 
TTTGGGCAGC TTATTGCCTT CACAATGTTT GTCCCAATTG TTCTCATGAA CTTACTGATT 
GGCTTGGCGG TTGGGGACAT TGCTGAGGTC CAGAAGCATG CGTCATTGAA GAGGATTGCT 
ATGCAGGTGG AACTTCATAC CAACTTAGAA AAAAAGCTGC CACTCTGGTA CTTACGCAAA 
GTGGATCAGA GGTCCACCAT CGTGTATCCA AATAGACCCA GGCACGGCAG GATGCTACGG 
TTTTTTCATT ACTTTCTTAA TATGCAAGAA ACACGACAAG AAGTACCAAA CATTGACACA 
TGCTTGGAAA TGGAAATATT GAAACAGAAA TATCGGCTGA AGGACCTCAC TTCCCTCTTG 
GAAAAGCAGC ATGAGCTCAT CAAACTCATC ATCCAGAAGA TGGAGATCAT CTCAGAGACA 
GAAGATGAAG ATAACCATTG CTCTTTCCAA GACAGGTTCA AGAAGGAGAG GCTGGAACAG 
ATGCACAGCA AGTGGAATTT TGTCTTAAAC GCAGTTAAGA CTAAAACACA TTGTTCTATT 
AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpa1 ( NM_001348288.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001335217.1 Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu27151
Clone ID Related Accession (Same CDS sequence)	XM_006495526.2 , NM_001348288.1
Accession Version	NM_001348288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily A member 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045771.1, KF460461.1 and AC121789.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAAAAGG AATTACTTCC TGGATTACAA CAATGCTCTG 
GAATGGGTTA TCTATACAAC TAGTATCATC TTCGTGTTGC CCTTGTTCCT CAACATCCCA 
GCGTATATGC AGTGGCAATG TGGAGCAATA GCGATATTCT TCTACTGGAT GAACTTCCTA 
CTGTATCTTC AAAGGTTTGA GAACTGTGGA ATTTTCATTG TTATGTTGGA GGTGATTTTT 
AAAACATTGC TGAGATCGAC CGGAGTGTTT ATCTTCCTCC TACTGGCTTT TGGCCTCAGC 
TTTTATGTTC TCCTGAATTT CCAAGATGCC TTCAGCACCC CATTGCTTTC CTTAATCCAG 
ACATTCAGTA TGATGCTAGG AGACATCAAT TATCGAGATG CCTTCCTAGA ACCATTGTTT 
AGAAATGAGT TGGCATACCC AGTCCTGACC TTTGGGCAGC TTATTGCCTT CACAATGTTT 
GTCCCAATTG TTCTCATGAA CTTACTGATT GGCTTGGCGG TTGGGGACAT TGCTGAGGTC 
CAGAAGCATG CGTCATTGAA GAGGATTGCT ATGCAGGTGG AACTTCATAC CAACTTAGAA 
AAAAAGCTGC CACTCTGGTA CTTACGCAAA GTGGATCAGA GGTCCACCAT CGTGTATCCA 
AATAGACCCA GGCACGGCAG GATGCTACGG TTTTTTCATT ACTTTCTTAA TATGCAAGAA 
ACACGACAAG AAGTACCAAA CATTGACACA TGCTTGGAAA TGGAAATATT GAAACAGAAA 
TATCGGCTGA AGGACCTCAC TTCCCTCTTG GAAAAGCAGC ATGAGCTCAT CAAACTCATC 
ATCCAGAAGA TGGAGATCAT CTCAGAGACA GAAGATGAAG ATAACCATTG CTCTTTCCAA 
GACAGGTTCA AGAAGGAGAG GCTGGAACAG ATGCACAGCA AGTGGAATTT TGTCTTAAAC 
GCAGTTAAGA CTAAAACACA TTGTTCTATT AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335217.1
CDS	28..3315
Translation	MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQKYRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSISHPDF

Target ORF information:

RefSeq Version	NM_001348288.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348288.1

ORF Insert Sequence:

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAAAAGG AATTACTTCC TGGATTACAA CAATGCTCTG 
GAATGGGTTA TCTATACAAC TAGTATCATC TTCGTGTTGC CCTTGTTCCT CAACATCCCA 
GCGTATATGC AGTGGCAATG TGGAGCAATA GCGATATTCT TCTACTGGAT GAACTTCCTA 
CTGTATCTTC AAAGGTTTGA GAACTGTGGA ATTTTCATTG TTATGTTGGA GGTGATTTTT 
AAAACATTGC TGAGATCGAC CGGAGTGTTT ATCTTCCTCC TACTGGCTTT TGGCCTCAGC 
TTTTATGTTC TCCTGAATTT CCAAGATGCC TTCAGCACCC CATTGCTTTC CTTAATCCAG 
ACATTCAGTA TGATGCTAGG AGACATCAAT TATCGAGATG CCTTCCTAGA ACCATTGTTT 
AGAAATGAGT TGGCATACCC AGTCCTGACC TTTGGGCAGC TTATTGCCTT CACAATGTTT 
GTCCCAATTG TTCTCATGAA CTTACTGATT GGCTTGGCGG TTGGGGACAT TGCTGAGGTC 
CAGAAGCATG CGTCATTGAA GAGGATTGCT ATGCAGGTGG AACTTCATAC CAACTTAGAA 
AAAAAGCTGC CACTCTGGTA CTTACGCAAA GTGGATCAGA GGTCCACCAT CGTGTATCCA 
AATAGACCCA GGCACGGCAG GATGCTACGG TTTTTTCATT ACTTTCTTAA TATGCAAGAA 
ACACGACAAG AAGTACCAAA CATTGACACA TGCTTGGAAA TGGAAATATT GAAACAGAAA 
TATCGGCTGA AGGACCTCAC TTCCCTCTTG GAAAAGCAGC ATGAGCTCAT CAAACTCATC 
ATCCAGAAGA TGGAGATCAT CTCAGAGACA GAAGATGAAG ATAACCATTG CTCTTTCCAA 
GACAGGTTCA AGAAGGAGAG GCTGGAACAG ATGCACAGCA AGTGGAATTT TGTCTTAAAC 
GCAGTTAAGA CTAAAACACA TTGTTCTATT AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpa1 ( XM_006495526.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495589.1 Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant X1, mRNA.

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CloneID	OMu27151
Clone ID Related Accession (Same CDS sequence)	XM_006495526.2 , NM_001348288.1
Accession Version	XM_006495526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily A member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAAAAGG AATTACTTCC TGGATTACAA CAATGCTCTG 
GAATGGGTTA TCTATACAAC TAGTATCATC TTCGTGTTGC CCTTGTTCCT CAACATCCCA 
GCGTATATGC AGTGGCAATG TGGAGCAATA GCGATATTCT TCTACTGGAT GAACTTCCTA 
CTGTATCTTC AAAGGTTTGA GAACTGTGGA ATTTTCATTG TTATGTTGGA GGTGATTTTT 
AAAACATTGC TGAGATCGAC CGGAGTGTTT ATCTTCCTCC TACTGGCTTT TGGCCTCAGC 
TTTTATGTTC TCCTGAATTT CCAAGATGCC TTCAGCACCC CATTGCTTTC CTTAATCCAG 
ACATTCAGTA TGATGCTAGG AGACATCAAT TATCGAGATG CCTTCCTAGA ACCATTGTTT 
AGAAATGAGT TGGCATACCC AGTCCTGACC TTTGGGCAGC TTATTGCCTT CACAATGTTT 
GTCCCAATTG TTCTCATGAA CTTACTGATT GGCTTGGCGG TTGGGGACAT TGCTGAGGTC 
CAGAAGCATG CGTCATTGAA GAGGATTGCT ATGCAGGTGG AACTTCATAC CAACTTAGAA 
AAAAAGCTGC CACTCTGGTA CTTACGCAAA GTGGATCAGA GGTCCACCAT CGTGTATCCA 
AATAGACCCA GGCACGGCAG GATGCTACGG TTTTTTCATT ACTTTCTTAA TATGCAAGAA 
ACACGACAAG AAGTACCAAA CATTGACACA TGCTTGGAAA TGGAAATATT GAAACAGAAA 
TATCGGCTGA AGGACCTCAC TTCCCTCTTG GAAAAGCAGC ATGAGCTCAT CAAACTCATC 
ATCCAGAAGA TGGAGATCAT CTCAGAGACA GAAGATGAAG ATAACCATTG CTCTTTCCAA 
GACAGGTTCA AGAAGGAGAG GCTGGAACAG ATGCACAGCA AGTGGAATTT TGTCTTAAAC 
GCAGTTAAGA CTAAAACACA TTGTTCTATT AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495589.1
CDS	180..3467
Translation	MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQKYRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSISHPDF

Target ORF information:

RefSeq Version	XM_006495526.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495526.2

ORF Insert Sequence:

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAAAAGG AATTACTTCC TGGATTACAA CAATGCTCTG 
GAATGGGTTA TCTATACAAC TAGTATCATC TTCGTGTTGC CCTTGTTCCT CAACATCCCA 
GCGTATATGC AGTGGCAATG TGGAGCAATA GCGATATTCT TCTACTGGAT GAACTTCCTA 
CTGTATCTTC AAAGGTTTGA GAACTGTGGA ATTTTCATTG TTATGTTGGA GGTGATTTTT 
AAAACATTGC TGAGATCGAC CGGAGTGTTT ATCTTCCTCC TACTGGCTTT TGGCCTCAGC 
TTTTATGTTC TCCTGAATTT CCAAGATGCC TTCAGCACCC CATTGCTTTC CTTAATCCAG 
ACATTCAGTA TGATGCTAGG AGACATCAAT TATCGAGATG CCTTCCTAGA ACCATTGTTT 
AGAAATGAGT TGGCATACCC AGTCCTGACC TTTGGGCAGC TTATTGCCTT CACAATGTTT 
GTCCCAATTG TTCTCATGAA CTTACTGATT GGCTTGGCGG TTGGGGACAT TGCTGAGGTC 
CAGAAGCATG CGTCATTGAA GAGGATTGCT ATGCAGGTGG AACTTCATAC CAACTTAGAA 
AAAAAGCTGC CACTCTGGTA CTTACGCAAA GTGGATCAGA GGTCCACCAT CGTGTATCCA 
AATAGACCCA GGCACGGCAG GATGCTACGG TTTTTTCATT ACTTTCTTAA TATGCAAGAA 
ACACGACAAG AAGTACCAAA CATTGACACA TGCTTGGAAA TGGAAATATT GAAACAGAAA 
TATCGGCTGA AGGACCTCAC TTCCCTCTTG GAAAAGCAGC ATGAGCTCAT CAAACTCATC 
ATCCAGAAGA TGGAGATCAT CTCAGAGACA GAAGATGAAG ATAACCATTG CTCTTTCCAA 
GACAGGTTCA AGAAGGAGAG GCTGGAACAG ATGCACAGCA AGTGGAATTT TGTCTTAAAC 
GCAGTTAAGA CTAAAACACA TTGTTCTATT AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpa1 ( NM_177781.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808449.1 Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 1, mRNA.

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CloneID	OMu22951
Clone ID Related Accession (Same CDS sequence)	NM_177781.4 , NM_177781.5
Accession Version	NM_177781.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily A member 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK045771.1 and AC121789.2. On Feb 8, 2017 this sequence version replaced NM_177781.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAATTCA TTTCCAATAA AAATATGTAT GATTCTAGTT 
TTTTTATCAA GTATATTTGG ATATTGCAAA GAAGTGATCC AAATTTTCCA ACAGAAAAGG 
AATTACTTCC TGGATTACAA CAATGCTCTG GAATGGGTTA TCTATACAAC TAGTATCATC 
TTCGTGTTGC CCTTGTTCCT CAACATCCCA GCGTATATGC AGTGGCAATG TGGAGCAATA 
GCGATATTCT TCTACTGGAT GAACTTCCTA CTGTATCTTC AAAGGTTTGA GAACTGTGGA 
ATTTTCATTG TTATGTTGGA GGTGATTTTT AAAACATTGC TGAGATCGAC CGGAGTGTTT 
ATCTTCCTCC TACTGGCTTT TGGCCTCAGC TTTTATGTTC TCCTGAATTT CCAAGATGCC 
TTCAGCACCC CATTGCTTTC CTTAATCCAG ACATTCAGTA TGATGCTAGG AGACATCAAT 
TATCGAGATG CCTTCCTAGA ACCATTGTTT AGAAATGAGT TGGCATACCC AGTCCTGACC 
TTTGGGCAGC TTATTGCCTT CACAATGTTT GTCCCAATTG TTCTCATGAA CTTACTGATT 
GGCTTGGCGG TTGGGGACAT TGCTGAGGTC CAGAAGCATG CGTCATTGAA GAGGATTGCT 
ATGCAGGTGG AACTTCATAC CAACTTAGAA AAAAAGCTGC CACTCTGGTA CTTACGCAAA 
GTGGATCAGA GGTCCACCAT CGTGTATCCA AATAGACCCA GGCACGGCAG GATGCTACGG 
TTTTTTCATT ACTTTCTTAA TATGCAAGAA ACACGACAAG AAGTACCAAA CATTGACACA 
TGCTTGGAAA TGGAAATATT GAAACAGAAA TATCGGCTGA AGGACCTCAC TTCCCTCTTG 
GAAAAGCAGC ATGAGCTCAT CAAACTCATC ATCCAGAAGA TGGAGATCAT CTCAGAGACA 
GAAGATGAAG ATAACCATTG CTCTTTCCAA GACAGGTTCA AGAAGGAGAG GCTGGAACAG 
ATGCACAGCA AGTGGAATTT TGTCTTAAAC GCAGTTAAGA CTAAAACACA TTGTTCTATT 
AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808449.1
CDS	28..3405
Translation	MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLNSFPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQKYRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSISHPDF

Target ORF information:

RefSeq Version	NM_177781.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily A, member 1 (Trpa1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177781.5

ORF Insert Sequence:

ATGAAGCGCG GCTTGAGGAG GATTCTGCTC CCGGAGGAAA GGAAGGAGGT CCAGGGCGTT 
GTCTATCGCG GCGTCGGGGA AGACATGGAC TGCTCCAAGG AATCCTTTAA GGTGGACATT 
GAAGGAGATA TGTGTAGATT AGAAGACTTC ATCAAGAACC GAAGAAAACT AAGCAAATAT 
GAGGATGAAA ATCTCTGTCC TCTGCATCAC GCAGCAGCAG AAGGTCAAGT TGAACTGATG 
GAACTGATCA TCAATGGTTC TTCGTGTGAA GTGCTGAATA TAATGGATGG TTATGGAAAT 
ACCCCACTGC ATTGTGCTGC AGAAAAAAAT CAAGTTGAAA GTGTAAAGTT TCTTCTCAGC 
CAAGGAGCAA ATCCAAACCT CCGAAATAGA AACATGATGT CACCCCTTCA CATAGCTGTG 
CATGGCATGT ACAACGAAGT GATCAAGGTG TTGACTGAGC ACAAGGCCAC TAACATCAAT 
TTAGAAGGAG AGAATGGGAA CACGGCTTTG ATGTCCACGT GTGCCAAAGA CAACAGTGAA 
GCTTTGCAAA TTTTGTTAGA AAAAGGAGCT AAGCTGTGTA AATCAAATAA GTGGGGAGAC 
TACCCTGTGC ACCAGGCAGC ATTTTCAGGT GCCAAAAAAT GCATGGAATT AATCTTAGCA 
TATGGTGAAA AGAACGGCTA CAGCAGGGAG ACTCACATTA ATTTTGTGAA TCACAAGAAA 
GCCAGCCCTC TCCACCTAGC AGTTCAAAGC GGAGACTTGG ACATGATTAA GATGTGCCTG 
GACAACGGTG CACACATCGA CATGATGGAG AATGCCAAAT GCATGGCCCT CCATTTTGCT 
GCAACCCAGG GAGCCACTGA CATCGTTAAG CTCATGATCT CATCCTATAC CGGAAGTAGT 
GATATTGTGA ATGCAGTTGA TGGCAATCAG GAGACCCTGC TTCACAGAGC CTCGTTATTT 
GATCACCATG ACCTGGCAGA ATACCTAATA TCAGTGGGAG CAGACATCAA CAGCACTGAT 
TCTGAAGGAC GCTCTCCACT TATTTTAGCA ACAGCTTCTG CATCCTGGAA CATTGTGAAT 
TTGCTCCTCT GTAAAGGTGC CAAAGTAGAC ATAAAAGATC ATCTTGGACG TAACTTTTTG 
CATTTGACTG TGCAGCAGCC TTATGGACTA AGAAATTTGC GGCCTGAGTT TATGCAGATG 
CAACACATCA AAGAGCTGGT GATGGATGAA GACAATGACG GATGCACACC TCTCCATTAT 
GCCTGTAGGC AGGGGGTTCC TGTCTCTGTA AATAACCTCC TTGGCTTCAA TGTGTCCATT 
CATAGCAAAA GTAAAGATAA GAAGTCGCCC CTGCATTTTG CAGCCAGTTA TGGGCGCATC 
AATACATGTC AGAGACTTCT GCAAGACATA AGTGATACGA GGCTTTTGAA TGAAGGGGAT 
CTCCATGGGA TGACCCCTCT CCACCTGGCA GCAAAAAATG GGCATGATAA AGTCGTTCAA 
CTCCTTCTGA AGAAAGGGGC CTTATTTCTC AGTGACCACA ATGGCTGGAC TGCTTTGCAT 
CACGCCTCCA TGGGTGGGTA CACTCAGACC ATGAAGGTCA TTCTTGATAC TAACTTGAAA 
TGCACAGACC GACTAGATGA AGAAGGGAAC ACAGCACTCC ACTTTGCAGC ACGGGAAGGC 
CATGCCAAGG CTGTTGCAAT GCTTTTGAGC TACAATGCTG ACATCCTCCT GAACAAGAAG 
CAAGCTTCCT TTCTGCATAT TGCCCTGCAC AATAAGCGCA AGGAAGTGGT TCTCACAACC 
ATCAGAAATA AAAGATGGGA TGAGTGTCTT CAAGTTTTCA CTCATAATTC TCCAAGCAAT 
CGATGTCCAA TCATGGAGAT GGTAGAATAC CTCCCCGAGT GCATGAAAGT TCTTTTAGAT 
TTCTGCATGA TACCTTCCAC AGAAGACAAG TCCTGTCAAG ACTACCATAT TGAGTATAAT 
TTCAAGTATC TCCAATGCCC ATTATCCATG ACCAAAAAAG TAGCACCTAC CCAGGATGTG 
GTATATGAGC CTCTTACAAT CCTCAATGTC ATGGTCCAAC ATAACCGCAT AGAACTCCTC 
AACCACCCTG TGTGTAGGGA GTACTTACTC ATGAAATGGT GTGCCTATGG ATTCAGAGCC 
CATATGATGA ACCTAGGATC TTATTGTCTT GGTCTCATAC CCATGACCCT TCTTGTTGTC 
AAAATACAGC CTGGAATGGC CTTCAATTCT ACTGGAATAA TCAATGGAAC TAGTAGTACT 
CATGAGGAAA GAATAGACAC TCTGAATTCA TTTCCAATAA AAATATGTAT GATTCTAGTT 
TTTTTATCAA GTATATTTGG ATATTGCAAA GAAGTGATCC AAATTTTCCA ACAGAAAAGG 
AATTACTTCC TGGATTACAA CAATGCTCTG GAATGGGTTA TCTATACAAC TAGTATCATC 
TTCGTGTTGC CCTTGTTCCT CAACATCCCA GCGTATATGC AGTGGCAATG TGGAGCAATA 
GCGATATTCT TCTACTGGAT GAACTTCCTA CTGTATCTTC AAAGGTTTGA GAACTGTGGA 
ATTTTCATTG TTATGTTGGA GGTGATTTTT AAAACATTGC TGAGATCGAC CGGAGTGTTT 
ATCTTCCTCC TACTGGCTTT TGGCCTCAGC TTTTATGTTC TCCTGAATTT CCAAGATGCC 
TTCAGCACCC CATTGCTTTC CTTAATCCAG ACATTCAGTA TGATGCTAGG AGACATCAAT 
TATCGAGATG CCTTCCTAGA ACCATTGTTT AGAAATGAGT TGGCATACCC AGTCCTGACC 
TTTGGGCAGC TTATTGCCTT CACAATGTTT GTCCCAATTG TTCTCATGAA CTTACTGATT 
GGCTTGGCGG TTGGGGACAT TGCTGAGGTC CAGAAGCATG CGTCATTGAA GAGGATTGCT 
ATGCAGGTGG AACTTCATAC CAACTTAGAA AAAAAGCTGC CACTCTGGTA CTTACGCAAA 
GTGGATCAGA GGTCCACCAT CGTGTATCCA AATAGACCCA GGCACGGCAG GATGCTACGG 
TTTTTTCATT ACTTTCTTAA TATGCAAGAA ACACGACAAG AAGTACCAAA CATTGACACA 
TGCTTGGAAA TGGAAATATT GAAACAGAAA TATCGGCTGA AGGACCTCAC TTCCCTCTTG 
GAAAAGCAGC ATGAGCTCAT CAAACTCATC ATCCAGAAGA TGGAGATCAT CTCAGAGACA 
GAAGATGAAG ATAACCATTG CTCTTTCCAA GACAGGTTCA AGAAGGAGAG GCTGGAACAG 
ATGCACAGCA AGTGGAATTT TGTCTTAAAC GCAGTTAAGA CTAAAACACA TTGTTCTATT 
AGCCACCCGG ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antimycin A-induced mitochondrial dysfunction activates vagal sensory neurons via ROS-dependent activation of TRPA1 and ROS-independent activation of TRPV1.
Brain research171594-105(2019 Jul)
Stanford KR,Hadley SH,Barannikov I,Ajmo JM,Bahia PK,Taylor-Clark TE

Methylglyoxal and a spinal TRPA1-AC1-Epac cascade facilitate pain in the db/db mouse model of type 2 diabetes.
Neurobiology of disease12776-86(2019 07)
Griggs RB,Santos DF,Laird DE,Doolen S,Donahue RR,Wessel CR,Fu W,Sinha GP,Wang P,Zhou J,Brings S,Fleming T,Nawroth PP,Susuki K,Taylor BK

Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc: Association with Inflammation and Matrix Remodeling.
International journal of molecular sciences20(7)(2019 Apr)
Kameda T,Zvick J,Vuk M,Sadowska A,Tam WK,Leung VY,Bölcskei K,Helyes Z,Applegate LA,Hausmann ON,Klasen J,Krupkova O,Wuertz-Kozak K

TRPA1 channel contributes to myocardial ischemia-reperfusion injury.
American journal of physiology. Heart and circulatory physiology316(4)H889-H899(2019 04)
Conklin DJ,Guo Y,Nystoriak MA,Jagatheesan G,Obal D,Kilfoil PJ,Hoetker JD,Guo L,Bolli R,Bhatnagar A

Transient Receptor Potential Ankyrin 1 Enhances Ovalbumin-Induced Acute Allergic Inflammation in Murine Models.
International archives of allergy and immunology178(3)238-247(2019)
Moilanen LJ,Hämäläinen M,Ilmarinen P,Kankaanranta H,Nieminen RM,Moilanen E,Lehtimäki L

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Trpa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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