EIF2AK4 gene cDNA ORF clone, Mus musculus(House mouse)

mRNA	Protein	Name
XM_017318909.1	XP_017174398.1	eIF-2-alpha kinase GCN2 isoform X1
XM_017318909.2	XP_017174398.1	eIF-2-alpha kinase GCN2 isoform X1
NM_013719.3	NP_038747.2	eIF-2-alpha kinase GCN2 isoform 1
XM_006499626.3	XP_006499689.1	eIF-2-alpha kinase GCN2 isoform X3
NM_001177806.1	NP_001171277.1	eIF-2-alpha kinase GCN2 isoform 2
XM_006499625.3	XP_006499688.1	eIF-2-alpha kinase GCN2 isoform X2
XM_006499625.4	XP_006499688.1	eIF-2-alpha kinase GCN2 isoform X2
XM_006499627.4	XP_006499690.1	eIF-2-alpha kinase GCN2 isoform X4
NM_001355383.1	NP_001342312.1	eIF-2-alpha kinase GCN2 isoform 3
XM_006499626.4	XP_006499689.1	eIF-2-alpha kinase GCN2 isoform X3
XM_006499627.3	XP_006499690.1	eIF-2-alpha kinase GCN2 isoform X4
XM_017318915.1	XP_017174404.1	eIF-2-alpha kinase GCN2 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30530	NM_001355383.1 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu30529	XM_006499627.4 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu30530	XM_017318915.1 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17698	NM_013719.3 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu17792	NM_001177806.1 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu17792	XM_006499625.4 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30528	XM_006499626.4 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80270	XM_017318909.2 Latest version!	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMu80270	XM_017318909.1	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu17792	XM_006499625.3	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30528	XM_006499626.3	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu30529	XM_006499627.3	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

EIF2AK4 ( NM_001355383.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001342312.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu30530
Clone ID Related Accession (Same CDS sequence)	NM_001355383.1 , XM_017318915.1
Accession Version	NM_001355383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929163.5. On Sep 15, 2017 this sequence version replaced XM_017318915.1. Transcript Variant: This variant (3) lacks three alternate internal exons in the 5' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.42515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342312.1
CDS	326..4702
Translation	MPTYIPRCERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	NM_001355383.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355383.1

ORF Insert Sequence:

ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499627.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499690.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30529
Clone ID Related Accession (Same CDS sequence)	XM_006499627.4 , XM_006499627.3
Accession Version	XM_006499627.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499627.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499690.1
CDS	126..4622
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499627.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499627.4

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499627.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499690.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

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CloneID	OMu30529
Clone ID Related Accession (Same CDS sequence)	XM_006499627.4 , XM_006499627.3
Accession Version	XM_006499627.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499690.1
CDS	128..4624
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499627.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499627.3

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_017318915.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174404.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X5, mRNA.

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CloneID	OMu30530
Clone ID Related Accession (Same CDS sequence)	NM_001355383.1 , XM_017318915.1
Accession Version	XM_017318915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	eIF-2-alpha kinase GCN2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174404.1
CDS	286..4662
Translation	MPTYIPRCERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_017318915.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318915.1

ORF Insert Sequence:

ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT 
CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA 
CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC 
TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG 
CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG 
CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG 
AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG 
AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC 
TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG 
AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA 
GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC 
TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA 
GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG 
CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC 
AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC 
TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG 
CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT 
CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC 
AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA 
GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT 
GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC 
CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC 
TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC 
TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA 
GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG 
AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC 
AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT 
TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT 
CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT 
GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC 
ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT 
CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC 
AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC 
CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG 
TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG 
GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT 
ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC 
AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT 
ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG 
GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG 
CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG 
AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC 
CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC 
GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA 
AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG 
GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA 
CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT 
CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT 
CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT 
CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA 
CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG 
ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT 
AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG 
CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC 
TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC 
TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC 
CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA 
TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT 
GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT 
GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG 
GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC 
TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG 
GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG 
CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA 
ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT 
CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC 
CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG 
ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA 
ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG 
TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA 
AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG 
GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( NM_013719.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_038747.2 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, mRNA.

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CloneID	OMu17698
Clone ID Related Accession (Same CDS sequence)	NM_013719.3
Accession Version	NM_013719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929163.5, AK013758.1, CO430666.1, CN527645.1, BG086385.2, CB723221.1, CF748039.1, BF453236.1, BF462783.1, W75391.1, BM250244.2 and AI324072.1. On Apr 28, 2010 this sequence version replaced NM_013719.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243533.1, AF193343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGCAACGTG AAATCCTCCA CGAGATTCAG 
AGAAGGAAAG AGGAGATCAA GGAAGAGAAG AAAAGGAAGG AAATGGCTAA GCAGGAACGT 
TTGGAAATCA CTAGCTTGAC AAACCAGGAC TATGCCTCTA AGAGAGACCC AGCAGGGCAC 
AGGGCAGCTG CCATCCTCCA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAAGCCCGG 
ACATACTCCT CAGGAAGGTC CAGGCGAGAA CGTCAGTATT CTGTGTGTAG TGGTGAACCC 
TCTCCTGGCT CGTGTGACAT CCTCCATTTC TCTGTGGGTA GCCCTGATCA GCTCATGGTG 
CACAAAGGGA GATGTGTTGG CAGTGATGAG CAGCTGGGAA AGGTGGTATA CAATGCTTTG 
GAAACAGCCA CGGGCAGCTT TGTCTTGCTG CATGAGTGGG TCCTTCAATG GCAGAAGATG 
GGCCCTTGCC TTACCAGTCA AGAGAAAGAG AAGATTGACA AGTGCAAAAG GCAGATTCAA 
GGAGCAGAAA CAGAATTCAG CTCCCTAGTG AAACTGAGCC ATCCAAATAT CGTCCGCTAC 
TTTGCGATGA ACTCCAGAGA GGAGGAGGAC TCCATTGTGA TCGACATTCT GGCAGAGCAC 
GTCAGTGGCA TCTCTCTGGC TACACACCTG AGCCACTCGG GCCCAGTCCC CGCCCACCAG 
CTGCGCAAGT ACACAGCCCA GCTCCTGGCA GGCCTGGATT ACCTACACAG CAACTCCGTG 
GTGCACAAGG TTCTGAGCGC GTCCAGTGTC TTGGTAGATG CCGAGGGCAC TGTCAAGATA 
ACAGACTACA GCATCTCTAA GCGTCTGGCA GACATTTGCA AGGAGGATGT ATTTGAGCAA 
GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG CCTTATAAAA CAGGAAAGAA AGGGGATGTG 
TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC AGCCAAGGAC AGGAGTGTGG GGAGTATCCG 
GTGACCATCC CCAGTGACCT GCCAGCTGAC TTCCAAGACT TCCTGAAGAA GTGTGTCTGC 
CTGGACGACA AGGAAAGATG GAGCCCTCAG CAGCTGCTGA AACACAGCTT TATAAACCCT 
CAACCAAAGC TGCCTTTGGT GGAGCAGAGT CCTGAAGACT CTGGGGGACA AGACTACATT 
GAGACCGTCA TTCCCAGCAA CCAGCTGCCC AGCGCTGCGT TCTTCAGTGA GACACAGAAA 
CAGTTCTCCC GGTACTTCAT TGAGTTTGAA GAACTACAAC TCCTAGGGAA AGGAGCTTTT 
GGAGCTGTCA TCAAGGTGCA AAACAAGCTT GATGGCTGCT GCTATGCTGT GAAGCGCATC 
CCTATCAACC CTGCAAGCAG ACACTTCCGC CGGATCAAGG GCGAGGTGAC ACTGCTATCG 
CGCCTGCACC ATGAGAACAT TGTGCGCTAC TACAACGCCT GGATCGAGCG GCATGAGCGC 
CCAGCGGTGC CAGGGACACC GCCCCCAGAC TGCACACCCC AGGCCCAGGA CAGCCCAGCC 
ACCTGCGGGA AAACATCAGG CGACACTGAA GAACTGGGCA GCGTGGAGGC TGCAGCACCG 
CCACCCATCC TCAGCAGCTC GGTGGAGTGG AGCACATCTG CAGAGCGTTC TACCAGCACC 
CGCTTCCCAG TCACGGGCCA GGATTCCAGC AGCGATGAGG AGGACGAGGA CGAGCGCGAT 
GGCGTCTTCT CCCAGTCCTT TCTACCTGCT TCAGATTCTG ACAGTGACAT CATCTTTGAC 
AATGAAGATG AAAACAGTAA AAGTCAAAAT CAGGATGAAG ACTGCAATCA AAAGGACGGT 
AGCCATGAGA TTGAGCCTTC AGTGACAGCT GAGGCTGTGC ACTACCTTTA TATCCAGATG 
GAGTACTGCG AGAAGAGCAC GCTGCGGGAC ACCATTGACC AGGGGCTGTT CCGAGACACC 
AGCCGGCTCT GGAGGCTTTT CCGAGAGATT CTGGATGGAT TAGCTTATAT CCATGAGAAA 
GGAATGATTC ATCGCGACTT GAAGCCTGTC AATATTTTTT TGGATTCTGA TGACCATGTG 
AAAATAGGTG ACTTTGGCCT GGCAACAGAC CATCTGGCCT TCACTGCTGA AGGTAAACAG 
GACGACCAGG CAGGTGACGG AGTGATTAAG TCAGACCCTT CAGGCCATTT GACTGGCATG 
GTTGGTACTG CTCTGTATGT AAGCCCTGAG GTCCAAGGAA GCACCAAGTC TGCATACAAC 
CAGAAAGTGG ATCTCTTCAG CCTGGGAATT ATCTTCTTTG AGATGTCTTA TCACCCGATG 
GTGACTGCCT CAGAAAGAAT TTTCGTTCTC AACCAACTTC GAGATCCCAC ATCGCCCAAG 
TTTCCAGACG ACTTCGATGA TGGAGAACAT ACAAAGCAGA AATCTGTCAT CTCCTGGCTG 
TTGAACCATG ATCCAGCTAA ACGGCCCACG GCCATGGAAC TGCTCAAGAG TGAGCTGCTC 
CCCCCGCCGC AGATGGAGGA GTCCGAGCTG CATGAAGTGC TGCACCACAC GCTGGCCAAC 
ATAGATGGGA AGGCCTACCG CACCATGATG AGTCAGATCT TCTGCCAGCA CATCTCCCCC 
GCTATAGACT ACACCTACGA CAGTGACATC CTGAAGGGCA ACTTCTTGAT TCGTACAGCC 
AAGATCCAGC AGCTTGTATG TGAAACCATC GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC 
CAGCTGTGCA CCCCACTGCT GCTTCCCCGA AACAGGCAAA TATATGAGCA CAACGAAGCG 
GCTTTGTTCA TGGATCACAG CGGCATGCTG GTGATGCTTC CGTTCGACCT GCGGGTCCCT 
TTTGCAAGAT ATGTGGCAAG AAATAACATA CTGAATTTAA AACGATACTG CATAGAGCGG 
GTGTTCAGAC CTCGGAAGTT AGACCGATTT CATCCCAAAG AACTCCTGGA ATGTGCATTT 
GATATCGTCA CTTCTACCAC CAACAGCTCT CTGCCCACTG CCGAAACCAT CTACACCATC 
TATGAAATAA TCCAGGAGTT TCCTGCACTT CAGGAAAGGA ATTACAGCAT TTACTTGAAC 
CATACGATGC TTCTGAAGGC AATACTCCTA CACTGTGGGA TCCCAGAGGA CAAGCTCAGC 
CAAGTCTACG TCATTCTGTA TGATGCTGTG ACAGAGAAGC TGACTAGGAG AGAAGTGGAA 
GCTAAATTCT GTAACCTGTC CTTGTCTTCT AATAGCCTGT GTAGACTCTA CAAGTTCATT 
GAGCAGAAGG GGGATTTGCA AGACTTAACG CCAACCATCA ACTCACTAAT AAAACAGAAA 
ACAGGCGTTG CTCAGTTGGT GAAGTATAGC TTAAAAGACC TCGAGGACGT CGTCGGGCTG 
CTGAAGAAAC TTGGCGTGAA GTTACAGGTC TCCATCAACC TGGGCTTGGT CTACAAGGTG 
CAGCAGCACA CTGGCATCAT CTTCCAGTTC CTGGCATTCA GCAAACGCAG GCAAAGGGTT 
GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA TACGACCTGC TGATTCCCAA GTTCAGAGGC 
CCACAGACTG TGGGGCCAGT CCCCACTGCT GTCGGTGTCA GCATAGCCAT AGACAAGATA 
TTTGCTGCTG TCCTCAACAT GGAAGAGCCT GTTACAGTGA GCTCCTGTGA CCTCCTGGTT 
GTCAGTGTTG GCCAGATGTC CATGTCCAGG GCCATCAACC TAACCCAGAA ACTGTGGACA 
GCGGGCATCA CTGCAGAAAT CATGTATGAC TGGTCCCAGT CCCAGGAAGA GTTACAAGAG 
TACTGCAGAC ACCATGAAAT CACCTATGTG GCTCTGGTCT CCGATAAAGA AGGAAGCCAT 
GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG CAAACAGAAA AGCGTGTATT GGAATCGGAT 
CTTGTGGATC ACGTTATGCA GAAACTAAGA ACCAAAGTTG GTGACGAAAG AAATTTCAGA 
GACGCTTCTG ATAATCTTGC CGTACAAACT CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT 
TTGTTTGAAA TCCATGGAAC TACAGTAGTC CCCAACGTGA TTGTTCTAGC ACCAGAAAAG 
CTGTCAGCCA GCACCAGGAG GCGACATGAG ATTCAGGTGC AAACCCGACT TCAGACTACA 
CTTGCCAACT TACATCAGAA AAGCAGTGAA ATTGAAATTT TGGCTGTGGA CCTACCCAAG 
GAAACAATCT TACAGTTTCT ATCATTAGAG TGGGATGCTG ATGAACAGGC ATTTAACACA 
ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA AAGCAAAGAT ACCTCAAACT CGTCTGCGAT 
GAAATTTATA ACATCAAAGT TGAAAAGAAG GTGTCAGTGC TGTTCCTGTA CAGCTACAGA 
GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038747.2
CDS	93..5039
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVYVQVELRVKCPPTYPDVVPEIDLKNAKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	NM_013719.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013719.3

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGCAACGTG AAATCCTCCA CGAGATTCAG 
AGAAGGAAAG AGGAGATCAA GGAAGAGAAG AAAAGGAAGG AAATGGCTAA GCAGGAACGT 
TTGGAAATCA CTAGCTTGAC AAACCAGGAC TATGCCTCTA AGAGAGACCC AGCAGGGCAC 
AGGGCAGCTG CCATCCTCCA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAAGCCCGG 
ACATACTCCT CAGGAAGGTC CAGGCGAGAA CGTCAGTATT CTGTGTGTAG TGGTGAACCC 
TCTCCTGGCT CGTGTGACAT CCTCCATTTC TCTGTGGGTA GCCCTGATCA GCTCATGGTG 
CACAAAGGGA GATGTGTTGG CAGTGATGAG CAGCTGGGAA AGGTGGTATA CAATGCTTTG 
GAAACAGCCA CGGGCAGCTT TGTCTTGCTG CATGAGTGGG TCCTTCAATG GCAGAAGATG 
GGCCCTTGCC TTACCAGTCA AGAGAAAGAG AAGATTGACA AGTGCAAAAG GCAGATTCAA 
GGAGCAGAAA CAGAATTCAG CTCCCTAGTG AAACTGAGCC ATCCAAATAT CGTCCGCTAC 
TTTGCGATGA ACTCCAGAGA GGAGGAGGAC TCCATTGTGA TCGACATTCT GGCAGAGCAC 
GTCAGTGGCA TCTCTCTGGC TACACACCTG AGCCACTCGG GCCCAGTCCC CGCCCACCAG 
CTGCGCAAGT ACACAGCCCA GCTCCTGGCA GGCCTGGATT ACCTACACAG CAACTCCGTG 
GTGCACAAGG TTCTGAGCGC GTCCAGTGTC TTGGTAGATG CCGAGGGCAC TGTCAAGATA 
ACAGACTACA GCATCTCTAA GCGTCTGGCA GACATTTGCA AGGAGGATGT ATTTGAGCAA 
GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG CCTTATAAAA CAGGAAAGAA AGGGGATGTG 
TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC AGCCAAGGAC AGGAGTGTGG GGAGTATCCG 
GTGACCATCC CCAGTGACCT GCCAGCTGAC TTCCAAGACT TCCTGAAGAA GTGTGTCTGC 
CTGGACGACA AGGAAAGATG GAGCCCTCAG CAGCTGCTGA AACACAGCTT TATAAACCCT 
CAACCAAAGC TGCCTTTGGT GGAGCAGAGT CCTGAAGACT CTGGGGGACA AGACTACATT 
GAGACCGTCA TTCCCAGCAA CCAGCTGCCC AGCGCTGCGT TCTTCAGTGA GACACAGAAA 
CAGTTCTCCC GGTACTTCAT TGAGTTTGAA GAACTACAAC TCCTAGGGAA AGGAGCTTTT 
GGAGCTGTCA TCAAGGTGCA AAACAAGCTT GATGGCTGCT GCTATGCTGT GAAGCGCATC 
CCTATCAACC CTGCAAGCAG ACACTTCCGC CGGATCAAGG GCGAGGTGAC ACTGCTATCG 
CGCCTGCACC ATGAGAACAT TGTGCGCTAC TACAACGCCT GGATCGAGCG GCATGAGCGC 
CCAGCGGTGC CAGGGACACC GCCCCCAGAC TGCACACCCC AGGCCCAGGA CAGCCCAGCC 
ACCTGCGGGA AAACATCAGG CGACACTGAA GAACTGGGCA GCGTGGAGGC TGCAGCACCG 
CCACCCATCC TCAGCAGCTC GGTGGAGTGG AGCACATCTG CAGAGCGTTC TACCAGCACC 
CGCTTCCCAG TCACGGGCCA GGATTCCAGC AGCGATGAGG AGGACGAGGA CGAGCGCGAT 
GGCGTCTTCT CCCAGTCCTT TCTACCTGCT TCAGATTCTG ACAGTGACAT CATCTTTGAC 
AATGAAGATG AAAACAGTAA AAGTCAAAAT CAGGATGAAG ACTGCAATCA AAAGGACGGT 
AGCCATGAGA TTGAGCCTTC AGTGACAGCT GAGGCTGTGC ACTACCTTTA TATCCAGATG 
GAGTACTGCG AGAAGAGCAC GCTGCGGGAC ACCATTGACC AGGGGCTGTT CCGAGACACC 
AGCCGGCTCT GGAGGCTTTT CCGAGAGATT CTGGATGGAT TAGCTTATAT CCATGAGAAA 
GGAATGATTC ATCGCGACTT GAAGCCTGTC AATATTTTTT TGGATTCTGA TGACCATGTG 
AAAATAGGTG ACTTTGGCCT GGCAACAGAC CATCTGGCCT TCACTGCTGA AGGTAAACAG 
GACGACCAGG CAGGTGACGG AGTGATTAAG TCAGACCCTT CAGGCCATTT GACTGGCATG 
GTTGGTACTG CTCTGTATGT AAGCCCTGAG GTCCAAGGAA GCACCAAGTC TGCATACAAC 
CAGAAAGTGG ATCTCTTCAG CCTGGGAATT ATCTTCTTTG AGATGTCTTA TCACCCGATG 
GTGACTGCCT CAGAAAGAAT TTTCGTTCTC AACCAACTTC GAGATCCCAC ATCGCCCAAG 
TTTCCAGACG ACTTCGATGA TGGAGAACAT ACAAAGCAGA AATCTGTCAT CTCCTGGCTG 
TTGAACCATG ATCCAGCTAA ACGGCCCACG GCCATGGAAC TGCTCAAGAG TGAGCTGCTC 
CCCCCGCCGC AGATGGAGGA GTCCGAGCTG CATGAAGTGC TGCACCACAC GCTGGCCAAC 
ATAGATGGGA AGGCCTACCG CACCATGATG AGTCAGATCT TCTGCCAGCA CATCTCCCCC 
GCTATAGACT ACACCTACGA CAGTGACATC CTGAAGGGCA ACTTCTTGAT TCGTACAGCC 
AAGATCCAGC AGCTTGTATG TGAAACCATC GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC 
CAGCTGTGCA CCCCACTGCT GCTTCCCCGA AACAGGCAAA TATATGAGCA CAACGAAGCG 
GCTTTGTTCA TGGATCACAG CGGCATGCTG GTGATGCTTC CGTTCGACCT GCGGGTCCCT 
TTTGCAAGAT ATGTGGCAAG AAATAACATA CTGAATTTAA AACGATACTG CATAGAGCGG 
GTGTTCAGAC CTCGGAAGTT AGACCGATTT CATCCCAAAG AACTCCTGGA ATGTGCATTT 
GATATCGTCA CTTCTACCAC CAACAGCTCT CTGCCCACTG CCGAAACCAT CTACACCATC 
TATGAAATAA TCCAGGAGTT TCCTGCACTT CAGGAAAGGA ATTACAGCAT TTACTTGAAC 
CATACGATGC TTCTGAAGGC AATACTCCTA CACTGTGGGA TCCCAGAGGA CAAGCTCAGC 
CAAGTCTACG TCATTCTGTA TGATGCTGTG ACAGAGAAGC TGACTAGGAG AGAAGTGGAA 
GCTAAATTCT GTAACCTGTC CTTGTCTTCT AATAGCCTGT GTAGACTCTA CAAGTTCATT 
GAGCAGAAGG GGGATTTGCA AGACTTAACG CCAACCATCA ACTCACTAAT AAAACAGAAA 
ACAGGCGTTG CTCAGTTGGT GAAGTATAGC TTAAAAGACC TCGAGGACGT CGTCGGGCTG 
CTGAAGAAAC TTGGCGTGAA GTTACAGGTC TCCATCAACC TGGGCTTGGT CTACAAGGTG 
CAGCAGCACA CTGGCATCAT CTTCCAGTTC CTGGCATTCA GCAAACGCAG GCAAAGGGTT 
GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA TACGACCTGC TGATTCCCAA GTTCAGAGGC 
CCACAGACTG TGGGGCCAGT CCCCACTGCT GTCGGTGTCA GCATAGCCAT AGACAAGATA 
TTTGCTGCTG TCCTCAACAT GGAAGAGCCT GTTACAGTGA GCTCCTGTGA CCTCCTGGTT 
GTCAGTGTTG GCCAGATGTC CATGTCCAGG GCCATCAACC TAACCCAGAA ACTGTGGACA 
GCGGGCATCA CTGCAGAAAT CATGTATGAC TGGTCCCAGT CCCAGGAAGA GTTACAAGAG 
TACTGCAGAC ACCATGAAAT CACCTATGTG GCTCTGGTCT CCGATAAAGA AGGAAGCCAT 
GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG CAAACAGAAA AGCGTGTATT GGAATCGGAT 
CTTGTGGATC ACGTTATGCA GAAACTAAGA ACCAAAGTTG GTGACGAAAG AAATTTCAGA 
GACGCTTCTG ATAATCTTGC CGTACAAACT CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT 
TTGTTTGAAA TCCATGGAAC TACAGTAGTC CCCAACGTGA TTGTTCTAGC ACCAGAAAAG 
CTGTCAGCCA GCACCAGGAG GCGACATGAG ATTCAGGTGC AAACCCGACT TCAGACTACA 
CTTGCCAACT TACATCAGAA AAGCAGTGAA ATTGAAATTT TGGCTGTGGA CCTACCCAAG 
GAAACAATCT TACAGTTTCT ATCATTAGAG TGGGATGCTG ATGAACAGGC ATTTAACACA 
ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA AAGCAAAGAT ACCTCAAACT CGTCTGCGAT 
GAAATTTATA ACATCAAAGT TGAAAAGAAG GTGTCAGTGC TGTTCCTGTA CAGCTACAGA 
GATGACTACT ACAGAATCCT ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( NM_001177806.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001171277.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, mRNA.

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CloneID	OMu17792
Clone ID Related Accession (Same CDS sequence)	NM_001177806.1 , XM_006499625.3 , XM_006499625.4
Accession Version	NM_001177806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC072637.1, AI643894.1 and AI324072.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate internal exon in the 5' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072637.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171277.1
CDS	125..4735
Translation	MPTYIPRCVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	NM_001177806.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177806.1

ORF Insert Sequence:

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499625.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499688.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu17792
Clone ID Related Accession (Same CDS sequence)	NM_001177806.1 , XM_006499625.3 , XM_006499625.4
Accession Version	XM_006499625.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499688.1
CDS	361..4971
Translation	MPTYIPRCVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499625.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499625.3

ORF Insert Sequence:

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499625.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499688.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

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CloneID	OMu17792
Clone ID Related Accession (Same CDS sequence)	NM_001177806.1 , XM_006499625.3 , XM_006499625.4
Accession Version	XM_006499625.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499625.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499688.1
CDS	359..4969
Translation	MPTYIPRCVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499625.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499625.4

ORF Insert Sequence:

ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG 
CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA 
CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG 
AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC 
TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC 
TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG 
TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG 
GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG 
GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG 
TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT 
GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG 
AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT 
GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC 
TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG 
GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA 
GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT 
TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT 
AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA 
GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA 
GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG 
CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA 
GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT 
GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA 
CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC 
TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC 
AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC 
GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA 
CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG 
GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA 
TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT 
GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT 
TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT 
GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT 
GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT 
GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT 
GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT 
TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG 
GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC 
CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA 
GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC 
TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA 
CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG 
CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG 
GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA 
GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG 
ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG 
GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC 
GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG 
CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG 
CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT 
TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC 
AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC 
ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA 
AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT 
GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG 
AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC 
CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC 
ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA 
GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC 
AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA 
TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC 
CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT 
GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA 
GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC 
AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC 
CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG 
GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA 
GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA 
GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG 
GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC 
GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG 
GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA 
ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT 
GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA 
AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA 
GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499626.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499689.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

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CloneID	OMu30528
Clone ID Related Accession (Same CDS sequence)	XM_006499626.3 , XM_006499626.4
Accession Version	XM_006499626.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA 
GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC 
ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT 
GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC 
TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC 
TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG 
AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC 
ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC 
CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA 
ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG 
AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC 
ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG 
TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG 
GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC 
AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT 
CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC 
GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC 
CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC 
AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG 
CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC 
ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC 
CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC 
ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC 
CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC 
CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG 
CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG 
AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC 
CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA 
GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC 
TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT 
GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG 
ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC 
GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC 
TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT 
CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT 
GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG 
GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT 
GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG 
GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC 
TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC 
GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT 
GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG 
CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG 
AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC 
TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG 
CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC 
ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC 
ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA 
TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA 
CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC 
ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA 
ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG 
CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC 
GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC 
TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG 
GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT 
GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA 
CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC 
ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG 
ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT 
GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT 
GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT 
GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC 
ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA 
CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC 
AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT 
CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT 
GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA 
ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC 
AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC 
TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC 
TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG 
CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT 
AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC 
TACAGAATCC TATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499689.1
CDS	128..4705
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499626.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499626.3

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA 
GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC 
ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT 
GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC 
TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC 
TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG 
AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC 
ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC 
CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA 
ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG 
AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC 
ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG 
TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG 
GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC 
AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT 
CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC 
GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC 
CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC 
AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG 
CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC 
ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC 
CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC 
ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC 
CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC 
CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG 
CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG 
AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC 
CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA 
GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC 
TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT 
GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG 
ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC 
GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC 
TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT 
CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT 
GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG 
GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT 
GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG 
GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC 
TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC 
GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT 
GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG 
CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG 
AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC 
TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG 
CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC 
ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC 
ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA 
TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA 
CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC 
ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA 
ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG 
CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC 
GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC 
TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG 
GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT 
GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA 
CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC 
ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG 
ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT 
GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT 
GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT 
GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC 
ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA 
CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC 
AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT 
CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT 
GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA 
ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC 
AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC 
TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC 
TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG 
CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT 
AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC 
TACAGAATCC TATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_006499626.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006499689.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

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CloneID	OMu30528
Clone ID Related Accession (Same CDS sequence)	XM_006499626.3 , XM_006499626.4
Accession Version	XM_006499626.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499626.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA 
GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC 
ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT 
GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC 
TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC 
TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG 
AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC 
ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC 
CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA 
ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG 
AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC 
ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG 
TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG 
GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC 
AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT 
CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC 
GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC 
CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC 
AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG 
CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC 
ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC 
CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC 
ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC 
CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC 
CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG 
CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG 
AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC 
CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA 
GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC 
TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT 
GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG 
ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC 
GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC 
TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT 
CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT 
GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG 
GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT 
GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG 
GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC 
TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC 
GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT 
GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG 
CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG 
AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC 
TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG 
CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC 
ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC 
ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA 
TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA 
CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC 
ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA 
ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG 
CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC 
GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC 
TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG 
GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT 
GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA 
CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC 
ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG 
ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT 
GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT 
GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT 
GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC 
ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA 
CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC 
AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT 
CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT 
GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA 
ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC 
AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC 
TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC 
TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG 
CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT 
AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC 
TACAGAATCC TATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499689.1
CDS	126..4703
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_006499626.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499626.4

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA 
GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC 
ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT 
GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC 
TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC 
TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG 
AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC 
ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC 
CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA 
ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG 
AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC 
ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG 
TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG 
GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC 
AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT 
CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC 
GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC 
CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC 
AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG 
CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC 
ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC 
CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC 
ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC 
CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC 
CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG 
CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG 
AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC 
CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA 
GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC 
TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT 
GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG 
ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC 
GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC 
TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT 
CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT 
GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG 
GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT 
GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG 
GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC 
TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC 
GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT 
GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG 
CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG 
AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC 
TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG 
CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC 
ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC 
ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA 
TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA 
CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC 
ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA 
ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG 
CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC 
GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC 
TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG 
GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT 
GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA 
CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC 
ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG 
ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT 
GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT 
GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT 
GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC 
ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA 
CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC 
AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT 
CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT 
GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA 
ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC 
AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC 
TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC 
TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG 
CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT 
AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC 
TACAGAATCC TATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_017318909.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174398.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80270
Clone ID Related Accession (Same CDS sequence)	XM_017318909.1 , XM_017318909.2
Accession Version	XM_017318909.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA 
AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT 
GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC 
AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC 
CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC 
AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT 
GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA 
GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC 
TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG 
GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT 
ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG 
CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG 
TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG 
CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC 
AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG 
TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG 
CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG 
AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG 
GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC 
CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT 
GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA 
AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA 
CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT 
GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG 
CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC 
GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG 
GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG 
GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT 
CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA 
AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA 
GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG 
CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC 
CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG 
AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG 
GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA 
GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA 
AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC 
CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT 
TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT 
GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA 
CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG 
TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC 
ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC 
AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT 
GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG 
CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC 
GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA 
AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA 
GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC 
AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT 
CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA 
ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT 
GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC 
TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA 
GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG 
AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG 
TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC 
TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC 
GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC 
CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG 
GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC 
ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC 
ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC 
ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG 
AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG 
AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC 
GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT 
ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG 
CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA 
AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA 
TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA 
CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT 
GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174398.1
CDS	129..4994
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVYVQVELRVKCPPTYPDVVPEIDLKNAKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_017318909.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318909.1

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA 
AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT 
GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC 
AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC 
CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC 
AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT 
GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA 
GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC 
TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG 
GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT 
ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG 
CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG 
TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG 
CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC 
AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG 
TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG 
CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG 
AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG 
GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC 
CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT 
GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA 
AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA 
CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT 
GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG 
CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC 
GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG 
GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG 
GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT 
CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA 
AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA 
GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG 
CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC 
CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG 
AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG 
GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA 
GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA 
AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC 
CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT 
TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT 
GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA 
CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG 
TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC 
ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC 
AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT 
GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG 
CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC 
GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA 
AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA 
GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC 
AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT 
CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA 
ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT 
GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC 
TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA 
GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG 
AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG 
TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC 
TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC 
GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC 
CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG 
GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC 
ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC 
ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC 
ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG 
AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG 
AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC 
GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT 
ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG 
CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA 
AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA 
TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA 
CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT 
GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_017318909.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174398.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

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CloneID	OMu80270
Clone ID Related Accession (Same CDS sequence)	XM_017318909.1 , XM_017318909.2
Accession Version	XM_017318909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA 
AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT 
GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC 
AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC 
CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC 
AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT 
GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA 
GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC 
TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG 
GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT 
ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG 
CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG 
TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG 
CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC 
AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG 
TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG 
CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG 
AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG 
GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC 
CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT 
GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA 
AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA 
CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT 
GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG 
CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC 
GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG 
GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG 
GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT 
CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA 
AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA 
GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG 
CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC 
CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG 
AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG 
GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA 
GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA 
AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC 
CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT 
TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT 
GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA 
CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG 
TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC 
ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC 
AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT 
GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG 
CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC 
GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA 
AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA 
GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC 
AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT 
CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA 
ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT 
GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC 
TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA 
GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG 
AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG 
TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC 
TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC 
GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC 
CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG 
GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC 
ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC 
ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC 
ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG 
AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG 
AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC 
GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT 
ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG 
CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA 
AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA 
TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA 
CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT 
GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174398.1
CDS	127..4992
Translation	MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVYVQVELRVKCPPTYPDVVPEIDLKNAKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_017318909.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318909.2

ORF Insert Sequence:

ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT 
CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC 
CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA 
CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT 
GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG 
GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC 
CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG 
TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA 
AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT 
GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC 
AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC 
CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC 
AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT 
GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA 
GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC 
TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG 
GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT 
ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG 
CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG 
TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG 
CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC 
AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG 
TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG 
CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG 
AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG 
GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC 
CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT 
GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA 
AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA 
CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT 
GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG 
CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC 
GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG 
GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG 
GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT 
CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA 
AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA 
GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG 
CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC 
CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG 
AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG 
GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA 
GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA 
AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC 
CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT 
TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT 
GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA 
CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG 
TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC 
ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC 
AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT 
GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG 
CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC 
GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA 
AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA 
GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC 
AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT 
CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA 
ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT 
GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC 
TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA 
GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG 
AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG 
TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC 
TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC 
GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC 
CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG 
GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC 
ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC 
ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC 
ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG 
AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG 
AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC 
GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT 
ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG 
CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA 
AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA 
TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA 
CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT 
GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EIF2AK4
Entrez Gene ID	27103
Full Name	eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms	2610011M03,GCN2,MGCN2
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 4 Names eIF-2-alpha kinase GCN2 GCN2 eIF2alpha kinase GCN2-like protein
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
Macaca mulatta (Rhesus monkey)	EIF2AK4	XP_002804763.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1
Mus musculus (house mouse)	Eif2ak4	NP_038747.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EIF2AK4 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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