Ropn1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ropn1l
Entrez Gene ID	252967
Full Name	ropporin 1-like
Synonyms	ASP,AV047578,Akapasp
General protein information	Preferred Names ropporin 1-like Names ropporin-1-like protein A-kinase anchoring protein-associated sperm protein AKAP-associated sperm protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_145852.2	NP_665851.2	ropporin-1-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ROPN1L	NP_001188395.1
Macaca mulatta (Rhesus monkey)	ROPN1L	XP_001085948.1
Mus musculus (house mouse)	Ropn1l	NP_665851.2
Rattus norvegicus (Norway rat)	Ropn1l	NP_001178015.1
Xenopus tropicalis (tropical clawed frog)	ropn1l	NP_001121440.1
Pan troglodytes (chimpanzee)	ROPN1L	XP_001146478.1
Bos taurus (cattle)	ROPN1L	NP_001069185.1
Danio rerio (zebrafish)	ropn1l	NP_001004624.1
Canis lupus familiaris (dog)	ROPN1L	XP_535790.3
Gallus gallus (chicken)	ROPN1L	XP_419011.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

FSCB phosphorylation regulates mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L.
Zhang X, Chen M, Yu R, Liu B, Tian Z, Liu S
American journal of translational research8(6)2776-82(2016)

Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity.
Fiedler SE, Dudiki T, Vijayaraghavan S, Carr DW
Biology of reproduction88(2)41(2013 Feb)

Loss of ASP but not ROPN1 reduces mammalian ciliary motility.
Fiedler SE, Sisson JH, Wyatt TA, Pavlik JA, Gambling TM, Carson JL, Carr DW
Cytoskeleton (Hoboken, N.J.)69(1)22-32(2012 Jan)

Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein.
Anway MD, Ravindranath N, Dym M, Griswold MD
Biology of reproduction66(6)1755-61(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ropn1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ropn1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20749	NM_145852.2 Latest version!	Mus musculus ropporin 1-like (Ropn1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ropn1l ( NM_145852.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_665851.2 Mus musculus ropporin 1-like (Ropn1l), mRNA.

Price & Availability↑

CloneID	OMu20749
Clone ID Related Accession (Same CDS sequence)	NM_145852.2
Accession Version	NM_145852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	ropporin-1-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131675.1 and AV039619.2. On Nov 19, 2006 this sequence version replaced NM_145852.1. ##Evidence-Data-START## Transcript exon combination :: BB667161.1, AK131675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCTGC CCGACACCAT GTTCTGCGCG CAGCAGATCC ACATCCCCCC GGAGCTGCCG 
GACATCCTCA AGCAGTTCAC CAAGGCTGCC ATCCGTACGC AGCCGGCGGA CGTGCTGCAG 
TGGTCGGCGG GGTATTTTTC AGCCCTGTCC AGAGGAGACC CACTTCCTGT AAAGGACAGA 
ATTGAAATGC CTGTGGCCAC ACAGAAGACT GACACAGGCC TGACCCAGGG ACTCCTGAAA 
GTCCTGCACA AGCAGTGTAG CCACAAGCAA TATGTGGAGT TAGCAGATCT TGAGAAGAAG 
TGGAAAAATT TGTGCCTGCC AGTAGAAAAG CTCAGAACGA TCCTGGAGCT GGATCCATGT 
GAGGACAAAA TAGAGTGGAT CAAGTTTTTA GCGCTCGGAT GCAGCTCCCT GGGCAGGACC 
CTGAACACGG CCATGAAGAA CGTGTGTGAG ATCCTCACCT CTGACCCGGA GGGCGGGCCT 
GCTCGCATCC CCTTTGAAAC CTTTGCCTAT GTTTACCAGT ACTTGTCTGG ACTGGACCCA 
GAACTCCCGG CCGTGGAGAC CGAGAACTAT CTTACCAGTT TAAGACTGAT GTCAGAATCT 
AGAAAGAATG GCATGATAGG ACTTTCGGAC TTCTTCGTTG GAAAAAAGAT AATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665851.2
CDS	147..803
Translation	MPLPDTMFCAQQIHIPPELPDILKQFTKAAIRTQPADVLQWSAGYFSALSRGDPLPVKDRIEMPVATQKTDTGLTQGLLKVLHKQCSHKQYVELADLEKKWKNLCLPVEKLRTILELDPCEDKIEWIKFLALGCSSLGRTLNTAMKNVCEILTSDPEGGPARIPFETFAYVYQYLSGLDPELPAVETENYLTSLRLMSESRKNGMIGLSDFFVGKKII

Target ORF information:

RefSeq Version	NM_145852.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ropporin 1-like (Ropn1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145852.2

ORF Insert Sequence:

ATGCCGCTGC CCGACACCAT GTTCTGCGCG CAGCAGATCC ACATCCCCCC GGAGCTGCCG 
GACATCCTCA AGCAGTTCAC CAAGGCTGCC ATCCGTACGC AGCCGGCGGA CGTGCTGCAG 
TGGTCGGCGG GGTATTTTTC AGCCCTGTCC AGAGGAGACC CACTTCCTGT AAAGGACAGA 
ATTGAAATGC CTGTGGCCAC ACAGAAGACT GACACAGGCC TGACCCAGGG ACTCCTGAAA 
GTCCTGCACA AGCAGTGTAG CCACAAGCAA TATGTGGAGT TAGCAGATCT TGAGAAGAAG 
TGGAAAAATT TGTGCCTGCC AGTAGAAAAG CTCAGAACGA TCCTGGAGCT GGATCCATGT 
GAGGACAAAA TAGAGTGGAT CAAGTTTTTA GCGCTCGGAT GCAGCTCCCT GGGCAGGACC 
CTGAACACGG CCATGAAGAA CGTGTGTGAG ATCCTCACCT CTGACCCGGA GGGCGGGCCT 
GCTCGCATCC CCTTTGAAAC CTTTGCCTAT GTTTACCAGT ACTTGTCTGG ACTGGACCCA 
GAACTCCCGG CCGTGGAGAC CGAGAACTAT CTTACCAGTT TAAGACTGAT GTCAGAATCT 
AGAAAGAATG GCATGATAGG ACTTTCGGAC TTCTTCGTTG GAAAAAAGAT AATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

FSCB phosphorylation regulates mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L.
American journal of translational research8(6)2776-82(2016)
Zhang X,Chen M,Yu R,Liu B,Tian Z,Liu S

Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity.
Biology of reproduction88(2)41(2013 Feb)
Fiedler SE,Dudiki T,Vijayaraghavan S,Carr DW

Loss of ASP but not ROPN1 reduces mammalian ciliary motility.
Cytoskeleton (Hoboken, N.J.)69(1)22-32(2012 Jan)
Fiedler SE,Sisson JH,Wyatt TA,Pavlik JA,Gambling TM,Carson JL,Carr DW

Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein.
Biology of reproduction66(6)1755-61(2002 Jun)
Anway MD,Ravindranath N,Dym M,Griswold MD

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Ropn1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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