Rhobtb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhobtb2
Entrez Gene ID	246710
Full Name	Rho-related BTB domain containing 2
Synonyms	Dbc2,E130206H14Rik,mKIAA0717
General protein information	Preferred Names Rho-related BTB domain containing 2 Names rho-related BTB domain-containing protein 2 deleted in breast cancer 2 gene protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316022.2	XP_017171511.1	rho-related BTB domain-containing protein 2 isoform X1
NM_153514.5	NP_705734.4	rho-related BTB domain-containing protein 2
XM_017316022.1	XP_017171511.1	rho-related BTB domain-containing protein 2 isoform X1
XM_030247818.1	XP_030103678.1	rho-related BTB domain-containing protein 2 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	rhobtb2	XP_002932752.2
Macaca mulatta (Rhesus monkey)	RHOBTB2	XP_001107431.2
Pan troglodytes (chimpanzee)	RHOBTB2	XP_003311691.1
Mus musculus (house mouse)	Rhobtb2	NP_705734.4
Danio rerio (zebrafish)	rhobtb2b	XP_694198.5
Bos taurus (cattle)	RHOBTB2	NP_001096574.1
Rattus norvegicus (Norway rat)	Rhobtb2	NP_001013151.1
Gallus gallus (chicken)	RHOBTB2	XP_001232710.3
Drosophila melanogaster (fruit fly)	RhoBTB	NP_001262108.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006474	XP_316514.4
Homo sapiens (human)	RHOBTB2	NP_001153508.1
Canis lupus familiaris (dog)	RHOBTB2	XP_005635764.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.
Lutz J, Grimm-Günter EM, Joshi P, Rivero F
Histochemistry and cell biology142(5)511-28(2014 Nov)

MUF1/leucine-rich repeat containing 41 (LRRC41), a substrate of RhoBTB-dependent cullin 3 ubiquitin ligase complexes, is a predominantly nuclear dimeric protein.
Schenková K, Lutz J, Kopp M, Ramos S, Rivero F
Journal of molecular biology422(5)659-673(2012 Oct)

High expression during neurogenesis but not mammogenesis of a murine homologue of the Deleted in Breast Cancer2/Rhobtb2 tumor suppressor.
St-Pierre B, Jiang Z, Egan SE, Zacksenhaus E
Gene expression patterns : GEP5(2)245-51(2004 Dec)

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)

Genomic organization and expression profile of the small GTPases of the RhoBTB family in human and mouse.
Ramos S, Khademi F, Somesh BP, Rivero F
Gene298(2)147-57(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rhobtb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhobtb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78767	XM_017316022.2 Latest version!	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu02518	NM_153514.5 Latest version!	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
OMu02518	XM_030247818.1 Latest version!	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
View Old Accession Versions >>
OMu78767	XM_017316022.1	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhobtb2 ( XM_017316022.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171511.1 Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78767
Clone ID Related Accession (Same CDS sequence)	XM_017316022.2 , XM_017316022.1
Accession Version	XM_017316022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	rho-related BTB domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AAAGAAAAGG CTGGTATAGC CCCTGGGAGA CCTCTCAGAA CAACATGTCC 
CAATTAATGG ATTCTGACAT GGATTATGAA AGGCCAAACG TAGAGACCAT CAAGTGCGTG 
GTGGTGGGGG ACAACGCCGT GGGCAAGACT AGGCTCATCT GTGCCCGGGC CTGCAATGCC 
ACCCTCACCC AGTACCAGTT GCTTGCCACC CATGTGCCCA CAGTGTGGGC CATTGACCAG 
TATCGTGTGT GCCAGGAGGT GCTAGAACGC TCCCGAGATG TGGTAGATGA CGTCAGCGTC 
TCCCTGCGCC TCTGGGACAC CTTCGGAGAC CACCACAAAG ACCGGCGTTT TGCTTATGGA 
AGATCCGACG TGGTGGTTTT GTGTTTCTCC ATCGCCAACC CCAATTCCCT CCACCATGTC 
AAGACCATGT GGTACCCAGA AATCAAGCAT TTCTGCCCCA GAGCTCCTGT CATTCTGGTG 
GGCTGCCAGC TGGACCTGCG CTATGCTGAC CTGGAAGCCG TTAACAGGGC CAGGAGACCT 
CTGGCTAGGC CCATCAAGCC CAATGAAATC CTCCCCCCAG AGAAGGGTCG GGAAGTCGCC 
AAAGAGCTGG GGATCCCTTA CTACGAGACC AGTGTGGTGG CCCAGTTTGG TATCAAGGAC 
GTCTTTGACA ATGCCATTCG GGCTGCGCTC ATCTCCCGCC GCCACCTGCA GTTCTGGAAA 
TCACATCTGC GCAATGTGCA GCGGCCTCTA CTGCAAGCAC CCTTCCTCCC CCCCAAGCCA 
CCGCCCCCCA TCATCGTGGT GCCCGATCCC CCCTCCAGCA GCGAGGAGTG CCCCGCCCAC 
CTCCTGGAAG ACCCGCTCTG TGCGGATGTC ATCCTGGTGC TGCAGGAGCG CGTGCGCATC 
TTTGCCCACA AAATCTACCT CTCCACCTCC TCCTCCAAAT TCTATGACTT ATTCCTCATG 
GACCTGAGTG AGGGGGAGCT GGGGGGCCCC TCAGGGTCAG GGGGGCCCCG CCCAGAGGAC 
CACCGGAGCC ACCCTGAACA ACACCACCAT CATCACCATC ACCACCATGG GCGGGACTTC 
CTGCTTCGGG CAGCCAGCTT TGACGTATGT GAGAGTGTGG ATGAGGCCGG CGGCTCTGGT 
CCGGCTGGCC TAAGGGCTTC AACCAGTGAC GGGATCTTAC GGGGTAATGG AACAGGGTAC 
CTGCCAGGCA GAGGACGTGT GCTGTCTTCC TGGAGCCGAG CTTTTGTGAG TATCCAGGAA 
GAGATGGCAG AGGATCCTCT GACCTTCAAA TCCCGGCTGA TGGTGGTGGT GAAGATGGAT 
AACTCCATTC AGCCGGGGCC CTTCCGGGCT GTTCTCAAGT ACCTGTACAC AGGGGAGCTA 
GGTGAGAATG AGCGGGACCT CATGCACATT GCTCACATTG CCGAGCTGCT AGAGGTCTTT 
GATCTACGAA TGATGGTGGC CAACATTCTC AACAACGAAG CCTTCATGAA TCAGGAGATC 
ACCAAGGCCT TCCATGTACG CCGGACCAAC CGGGTTAAGG AGTGTTTGGC AAAGGGCACC 
TTTTCAGATG TGACCTTCAT CCTGGATGAT GGGACCATCA GCGCCCATAA GCCCCTGCTG 
ATTTCCAGCT GTGACTGGAT GGCTGCCATG TTTGGGGGGC CATTTGTGGA GAGTTCTACC 
AGGGAGGTGG TGTTTCCATA CACAAGCAAG AGCTGCATGA GAGCTGTGTT GGAGTACCTC 
TATACAGGCA TGTTCACCTC CAGCCCAGAC CTGGATGATA TGAAGCTCAT TGTCTTGGCT 
AACCGCCTCT GCCTGCCGCA CTTGGTTGCC CTCACAGAGC AGTACACAGT GACTGGACTG 
ATGGAGGCGA CCCAGATGAT GGTGGACATC GATGGGGATG TCCTTGTATT CTTGGAATTG 
GCACAGTTCC ACTGCGCGTA CCAGTTGGCC GACTGGTGTC TTCACCATAT CTGCACCAAC 
TACAACAACG TGTGCCGCAA GTTCCCCAGA GACATGAAGG CTATGTCTCC AGAAAACCAG 
GAGTACTTTG AGAAACACCG GTGGCCGCCA GTCTGGTACC TGAAAGAAGA GGACCACTAT 
CAGCGTGCGC GGAAGGAGCG AGAGAAAGAG GACTATTTAC ACCTGCGGAG GCAGCCCAAG 
CGGCGGTGGC TGTTTTGGAA CAGTCCCTCA TCACCGTCCT CCTCAGCAGC GGGCTCAGCA 
TCCCCCTCCT CCTCCTCCTC GGCTGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171511.1
CDS	876..3128
Translation	MGRQRKGWYSPWETSQNNMSQLMDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSGSGGPRPEDHRSHPEQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTFKSRLMVVVKMDNSIQPGPFRAVLKYLYTGELGENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIVLANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLRRQPKRRWLFWNSPSSPSSSAAGSASPSSSSSAVV

Target ORF information:

RefSeq Version	XM_017316022.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316022.2

ORF Insert Sequence:

ATGGGAAGAC AAAGAAAAGG CTGGTATAGC CCCTGGGAGA CCTCTCAGAA CAACATGTCC 
CAATTAATGG ATTCTGACAT GGATTATGAA AGGCCAAACG TAGAGACCAT CAAGTGCGTG 
GTGGTGGGGG ACAACGCCGT GGGCAAGACT AGGCTCATCT GTGCCCGGGC CTGCAATGCC 
ACCCTCACCC AGTACCAGTT GCTTGCCACC CATGTGCCCA CAGTGTGGGC CATTGACCAG 
TATCGTGTGT GCCAGGAGGT GCTAGAACGC TCCCGAGATG TGGTAGATGA CGTCAGCGTC 
TCCCTGCGCC TCTGGGACAC CTTCGGAGAC CACCACAAAG ACCGGCGTTT TGCTTATGGA 
AGATCCGACG TGGTGGTTTT GTGTTTCTCC ATCGCCAACC CCAATTCCCT CCACCATGTC 
AAGACCATGT GGTACCCAGA AATCAAGCAT TTCTGCCCCA GAGCTCCTGT CATTCTGGTG 
GGCTGCCAGC TGGACCTGCG CTATGCTGAC CTGGAAGCCG TTAACAGGGC CAGGAGACCT 
CTGGCTAGGC CCATCAAGCC CAATGAAATC CTCCCCCCAG AGAAGGGTCG GGAAGTCGCC 
AAAGAGCTGG GGATCCCTTA CTACGAGACC AGTGTGGTGG CCCAGTTTGG TATCAAGGAC 
GTCTTTGACA ATGCCATTCG GGCTGCGCTC ATCTCCCGCC GCCACCTGCA GTTCTGGAAA 
TCACATCTGC GCAATGTGCA GCGGCCTCTA CTGCAAGCAC CCTTCCTCCC CCCCAAGCCA 
CCGCCCCCCA TCATCGTGGT GCCCGATCCC CCCTCCAGCA GCGAGGAGTG CCCCGCCCAC 
CTCCTGGAAG ACCCGCTCTG TGCGGATGTC ATCCTGGTGC TGCAGGAGCG CGTGCGCATC 
TTTGCCCACA AAATCTACCT CTCCACCTCC TCCTCCAAAT TCTATGACTT ATTCCTCATG 
GACCTGAGTG AGGGGGAGCT GGGGGGCCCC TCAGGGTCAG GGGGGCCCCG CCCAGAGGAC 
CACCGGAGCC ACCCTGAACA ACACCACCAT CATCACCATC ACCACCATGG GCGGGACTTC 
CTGCTTCGGG CAGCCAGCTT TGACGTATGT GAGAGTGTGG ATGAGGCCGG CGGCTCTGGT 
CCGGCTGGCC TAAGGGCTTC AACCAGTGAC GGGATCTTAC GGGGTAATGG AACAGGGTAC 
CTGCCAGGCA GAGGACGTGT GCTGTCTTCC TGGAGCCGAG CTTTTGTGAG TATCCAGGAA 
GAGATGGCAG AGGATCCTCT GACCTTCAAA TCCCGGCTGA TGGTGGTGGT GAAGATGGAT 
AACTCCATTC AGCCGGGGCC CTTCCGGGCT GTTCTCAAGT ACCTGTACAC AGGGGAGCTA 
GGTGAGAATG AGCGGGACCT CATGCACATT GCTCACATTG CCGAGCTGCT AGAGGTCTTT 
GATCTACGAA TGATGGTGGC CAACATTCTC AACAACGAAG CCTTCATGAA TCAGGAGATC 
ACCAAGGCCT TCCATGTACG CCGGACCAAC CGGGTTAAGG AGTGTTTGGC AAAGGGCACC 
TTTTCAGATG TGACCTTCAT CCTGGATGAT GGGACCATCA GCGCCCATAA GCCCCTGCTG 
ATTTCCAGCT GTGACTGGAT GGCTGCCATG TTTGGGGGGC CATTTGTGGA GAGTTCTACC 
AGGGAGGTGG TGTTTCCATA CACAAGCAAG AGCTGCATGA GAGCTGTGTT GGAGTACCTC 
TATACAGGCA TGTTCACCTC CAGCCCAGAC CTGGATGATA TGAAGCTCAT TGTCTTGGCT 
AACCGCCTCT GCCTGCCGCA CTTGGTTGCC CTCACAGAGC AGTACACAGT GACTGGACTG 
ATGGAGGCGA CCCAGATGAT GGTGGACATC GATGGGGATG TCCTTGTATT CTTGGAATTG 
GCACAGTTCC ACTGCGCGTA CCAGTTGGCC GACTGGTGTC TTCACCATAT CTGCACCAAC 
TACAACAACG TGTGCCGCAA GTTCCCCAGA GACATGAAGG CTATGTCTCC AGAAAACCAG 
GAGTACTTTG AGAAACACCG GTGGCCGCCA GTCTGGTACC TGAAAGAAGA GGACCACTAT 
CAGCGTGCGC GGAAGGAGCG AGAGAAAGAG GACTATTTAC ACCTGCGGAG GCAGCCCAAG 
CGGCGGTGGC TGTTTTGGAA CAGTCCCTCA TCACCGTCCT CCTCAGCAGC GGGCTCAGCA 
TCCCCCTCCT CCTCCTCCTC GGCTGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhobtb2 ( XM_017316022.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171511.1 Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78767
Clone ID Related Accession (Same CDS sequence)	XM_017316022.2 , XM_017316022.1
Accession Version	XM_017316022.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	rho-related BTB domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AAAGAAAAGG CTGGTATAGC CCCTGGGAGA CCTCTCAGAA CAACATGTCC 
CAATTAATGG ATTCTGACAT GGATTATGAA AGGCCAAACG TAGAGACCAT CAAGTGCGTG 
GTGGTGGGGG ACAACGCCGT GGGCAAGACT AGGCTCATCT GTGCCCGGGC CTGCAATGCC 
ACCCTCACCC AGTACCAGTT GCTTGCCACC CATGTGCCCA CAGTGTGGGC CATTGACCAG 
TATCGTGTGT GCCAGGAGGT GCTAGAACGC TCCCGAGATG TGGTAGATGA CGTCAGCGTC 
TCCCTGCGCC TCTGGGACAC CTTCGGAGAC CACCACAAAG ACCGGCGTTT TGCTTATGGA 
AGATCCGACG TGGTGGTTTT GTGTTTCTCC ATCGCCAACC CCAATTCCCT CCACCATGTC 
AAGACCATGT GGTACCCAGA AATCAAGCAT TTCTGCCCCA GAGCTCCTGT CATTCTGGTG 
GGCTGCCAGC TGGACCTGCG CTATGCTGAC CTGGAAGCCG TTAACAGGGC CAGGAGACCT 
CTGGCTAGGC CCATCAAGCC CAATGAAATC CTCCCCCCAG AGAAGGGTCG GGAAGTCGCC 
AAAGAGCTGG GGATCCCTTA CTACGAGACC AGTGTGGTGG CCCAGTTTGG TATCAAGGAC 
GTCTTTGACA ATGCCATTCG GGCTGCGCTC ATCTCCCGCC GCCACCTGCA GTTCTGGAAA 
TCACATCTGC GCAATGTGCA GCGGCCTCTA CTGCAAGCAC CCTTCCTCCC CCCCAAGCCA 
CCGCCCCCCA TCATCGTGGT GCCCGATCCC CCCTCCAGCA GCGAGGAGTG CCCCGCCCAC 
CTCCTGGAAG ACCCGCTCTG TGCGGATGTC ATCCTGGTGC TGCAGGAGCG CGTGCGCATC 
TTTGCCCACA AAATCTACCT CTCCACCTCC TCCTCCAAAT TCTATGACTT ATTCCTCATG 
GACCTGAGTG AGGGGGAGCT GGGGGGCCCC TCAGGGTCAG GGGGGCCCCG CCCAGAGGAC 
CACCGGAGCC ACCCTGAACA ACACCACCAT CATCACCATC ACCACCATGG GCGGGACTTC 
CTGCTTCGGG CAGCCAGCTT TGACGTATGT GAGAGTGTGG ATGAGGCCGG CGGCTCTGGT 
CCGGCTGGCC TAAGGGCTTC AACCAGTGAC GGGATCTTAC GGGGTAATGG AACAGGGTAC 
CTGCCAGGCA GAGGACGTGT GCTGTCTTCC TGGAGCCGAG CTTTTGTGAG TATCCAGGAA 
GAGATGGCAG AGGATCCTCT GACCTTCAAA TCCCGGCTGA TGGTGGTGGT GAAGATGGAT 
AACTCCATTC AGCCGGGGCC CTTCCGGGCT GTTCTCAAGT ACCTGTACAC AGGGGAGCTA 
GGTGAGAATG AGCGGGACCT CATGCACATT GCTCACATTG CCGAGCTGCT AGAGGTCTTT 
GATCTACGAA TGATGGTGGC CAACATTCTC AACAACGAAG CCTTCATGAA TCAGGAGATC 
ACCAAGGCCT TCCATGTACG CCGGACCAAC CGGGTTAAGG AGTGTTTGGC AAAGGGCACC 
TTTTCAGATG TGACCTTCAT CCTGGATGAT GGGACCATCA GCGCCCATAA GCCCCTGCTG 
ATTTCCAGCT GTGACTGGAT GGCTGCCATG TTTGGGGGGC CATTTGTGGA GAGTTCTACC 
AGGGAGGTGG TGTTTCCATA CACAAGCAAG AGCTGCATGA GAGCTGTGTT GGAGTACCTC 
TATACAGGCA TGTTCACCTC CAGCCCAGAC CTGGATGATA TGAAGCTCAT TGTCTTGGCT 
AACCGCCTCT GCCTGCCGCA CTTGGTTGCC CTCACAGAGC AGTACACAGT GACTGGACTG 
ATGGAGGCGA CCCAGATGAT GGTGGACATC GATGGGGATG TCCTTGTATT CTTGGAATTG 
GCACAGTTCC ACTGCGCGTA CCAGTTGGCC GACTGGTGTC TTCACCATAT CTGCACCAAC 
TACAACAACG TGTGCCGCAA GTTCCCCAGA GACATGAAGG CTATGTCTCC AGAAAACCAG 
GAGTACTTTG AGAAACACCG GTGGCCGCCA GTCTGGTACC TGAAAGAAGA GGACCACTAT 
CAGCGTGCGC GGAAGGAGCG AGAGAAAGAG GACTATTTAC ACCTGCGGAG GCAGCCCAAG 
CGGCGGTGGC TGTTTTGGAA CAGTCCCTCA TCACCGTCCT CCTCAGCAGC GGGCTCAGCA 
TCCCCCTCCT CCTCCTCCTC GGCTGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171511.1
CDS	13..2265
Translation	MGRQRKGWYSPWETSQNNMSQLMDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSGSGGPRPEDHRSHPEQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTFKSRLMVVVKMDNSIQPGPFRAVLKYLYTGELGENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIVLANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLRRQPKRRWLFWNSPSSPSSSAAGSASPSSSSSAVV

Target ORF information:

RefSeq Version	XM_017316022.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316022.1

ORF Insert Sequence:

ATGGGAAGAC AAAGAAAAGG CTGGTATAGC CCCTGGGAGA CCTCTCAGAA CAACATGTCC 
CAATTAATGG ATTCTGACAT GGATTATGAA AGGCCAAACG TAGAGACCAT CAAGTGCGTG 
GTGGTGGGGG ACAACGCCGT GGGCAAGACT AGGCTCATCT GTGCCCGGGC CTGCAATGCC 
ACCCTCACCC AGTACCAGTT GCTTGCCACC CATGTGCCCA CAGTGTGGGC CATTGACCAG 
TATCGTGTGT GCCAGGAGGT GCTAGAACGC TCCCGAGATG TGGTAGATGA CGTCAGCGTC 
TCCCTGCGCC TCTGGGACAC CTTCGGAGAC CACCACAAAG ACCGGCGTTT TGCTTATGGA 
AGATCCGACG TGGTGGTTTT GTGTTTCTCC ATCGCCAACC CCAATTCCCT CCACCATGTC 
AAGACCATGT GGTACCCAGA AATCAAGCAT TTCTGCCCCA GAGCTCCTGT CATTCTGGTG 
GGCTGCCAGC TGGACCTGCG CTATGCTGAC CTGGAAGCCG TTAACAGGGC CAGGAGACCT 
CTGGCTAGGC CCATCAAGCC CAATGAAATC CTCCCCCCAG AGAAGGGTCG GGAAGTCGCC 
AAAGAGCTGG GGATCCCTTA CTACGAGACC AGTGTGGTGG CCCAGTTTGG TATCAAGGAC 
GTCTTTGACA ATGCCATTCG GGCTGCGCTC ATCTCCCGCC GCCACCTGCA GTTCTGGAAA 
TCACATCTGC GCAATGTGCA GCGGCCTCTA CTGCAAGCAC CCTTCCTCCC CCCCAAGCCA 
CCGCCCCCCA TCATCGTGGT GCCCGATCCC CCCTCCAGCA GCGAGGAGTG CCCCGCCCAC 
CTCCTGGAAG ACCCGCTCTG TGCGGATGTC ATCCTGGTGC TGCAGGAGCG CGTGCGCATC 
TTTGCCCACA AAATCTACCT CTCCACCTCC TCCTCCAAAT TCTATGACTT ATTCCTCATG 
GACCTGAGTG AGGGGGAGCT GGGGGGCCCC TCAGGGTCAG GGGGGCCCCG CCCAGAGGAC 
CACCGGAGCC ACCCTGAACA ACACCACCAT CATCACCATC ACCACCATGG GCGGGACTTC 
CTGCTTCGGG CAGCCAGCTT TGACGTATGT GAGAGTGTGG ATGAGGCCGG CGGCTCTGGT 
CCGGCTGGCC TAAGGGCTTC AACCAGTGAC GGGATCTTAC GGGGTAATGG AACAGGGTAC 
CTGCCAGGCA GAGGACGTGT GCTGTCTTCC TGGAGCCGAG CTTTTGTGAG TATCCAGGAA 
GAGATGGCAG AGGATCCTCT GACCTTCAAA TCCCGGCTGA TGGTGGTGGT GAAGATGGAT 
AACTCCATTC AGCCGGGGCC CTTCCGGGCT GTTCTCAAGT ACCTGTACAC AGGGGAGCTA 
GGTGAGAATG AGCGGGACCT CATGCACATT GCTCACATTG CCGAGCTGCT AGAGGTCTTT 
GATCTACGAA TGATGGTGGC CAACATTCTC AACAACGAAG CCTTCATGAA TCAGGAGATC 
ACCAAGGCCT TCCATGTACG CCGGACCAAC CGGGTTAAGG AGTGTTTGGC AAAGGGCACC 
TTTTCAGATG TGACCTTCAT CCTGGATGAT GGGACCATCA GCGCCCATAA GCCCCTGCTG 
ATTTCCAGCT GTGACTGGAT GGCTGCCATG TTTGGGGGGC CATTTGTGGA GAGTTCTACC 
AGGGAGGTGG TGTTTCCATA CACAAGCAAG AGCTGCATGA GAGCTGTGTT GGAGTACCTC 
TATACAGGCA TGTTCACCTC CAGCCCAGAC CTGGATGATA TGAAGCTCAT TGTCTTGGCT 
AACCGCCTCT GCCTGCCGCA CTTGGTTGCC CTCACAGAGC AGTACACAGT GACTGGACTG 
ATGGAGGCGA CCCAGATGAT GGTGGACATC GATGGGGATG TCCTTGTATT CTTGGAATTG 
GCACAGTTCC ACTGCGCGTA CCAGTTGGCC GACTGGTGTC TTCACCATAT CTGCACCAAC 
TACAACAACG TGTGCCGCAA GTTCCCCAGA GACATGAAGG CTATGTCTCC AGAAAACCAG 
GAGTACTTTG AGAAACACCG GTGGCCGCCA GTCTGGTACC TGAAAGAAGA GGACCACTAT 
CAGCGTGCGC GGAAGGAGCG AGAGAAAGAG GACTATTTAC ACCTGCGGAG GCAGCCCAAG 
CGGCGGTGGC TGTTTTGGAA CAGTCCCTCA TCACCGTCCT CCTCAGCAGC GGGCTCAGCA 
TCCCCCTCCT CCTCCTCCTC GGCTGTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhobtb2 ( NM_153514.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705734.4 Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), mRNA.

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CloneID	OMu02518
Clone ID Related Accession (Same CDS sequence)	NM_153514.5 , XM_030247818.1
Accession Version	NM_153514.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	rho-related BTB domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162936.3. On Mar 27, 2008 this sequence version replaced NM_153514.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK081100.1, AK038196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCTG ACATGGATTA TGAAAGGCCA AACGTAGAGA CCATCAAGTG CGTGGTGGTG 
GGGGACAACG CCGTGGGCAA GACTAGGCTC ATCTGTGCCC GGGCCTGCAA TGCCACCCTC 
ACCCAGTACC AGTTGCTTGC CACCCATGTG CCCACAGTGT GGGCCATTGA CCAGTATCGT 
GTGTGCCAGG AGGTGCTAGA ACGCTCCCGA GATGTGGTAG ATGACGTCAG CGTCTCCCTG 
CGCCTCTGGG ACACCTTCGG AGACCACCAC AAAGACCGGC GTTTTGCTTA TGGAAGATCC 
GACGTGGTGG TTTTGTGTTT CTCCATCGCC AACCCCAATT CCCTCCACCA TGTCAAGACC 
ATGTGGTACC CAGAAATCAA GCATTTCTGC CCCAGAGCTC CTGTCATTCT GGTGGGCTGC 
CAGCTGGACC TGCGCTATGC TGACCTGGAA GCCGTTAACA GGGCCAGGAG ACCTCTGGCT 
AGGCCCATCA AGCCCAATGA AATCCTCCCC CCAGAGAAGG GTCGGGAAGT CGCCAAAGAG 
CTGGGGATCC CTTACTACGA GACCAGTGTG GTGGCCCAGT TTGGTATCAA GGACGTCTTT 
GACAATGCCA TTCGGGCTGC GCTCATCTCC CGCCGCCACC TGCAGTTCTG GAAATCACAT 
CTGCGCAATG TGCAGCGGCC TCTACTGCAA GCACCCTTCC TCCCCCCCAA GCCACCGCCC 
CCCATCATCG TGGTGCCCGA TCCCCCCTCC AGCAGCGAGG AGTGCCCCGC CCACCTCCTG 
GAAGACCCGC TCTGTGCGGA TGTCATCCTG GTGCTGCAGG AGCGCGTGCG CATCTTTGCC 
CACAAAATCT ACCTCTCCAC CTCCTCCTCC AAATTCTATG ACTTATTCCT CATGGACCTG 
AGTGAGGGGG AGCTGGGGGG CCCCTCAGGG TCAGGGGGGC CCCGCCCAGA GGACCACCGG 
AGCCACCCTG AACAACACCA CCATCATCAC CATCACCACC ATGGGCGGGA CTTCCTGCTT 
CGGGCAGCCA GCTTTGACGT ATGTGAGAGT GTGGATGAGG CCGGCGGCTC TGGTCCGGCT 
GGCCTAAGGG CTTCAACCAG TGACGGGATC TTACGGGGTA ATGGAACAGG GTACCTGCCA 
GGCAGAGGAC GTGTGCTGTC TTCCTGGAGC CGAGCTTTTG TGAGTATCCA GGAAGAGATG 
GCAGAGGATC CTCTGACCTT CAAATCCCGG CTGATGGTGG TGGTGAAGAT GGATAACTCC 
ATTCAGCCGG GGCCCTTCCG GGCTGTTCTC AAGTACCTGT ACACAGGGGA GCTAGGTGAG 
AATGAGCGGG ACCTCATGCA CATTGCTCAC ATTGCCGAGC TGCTAGAGGT CTTTGATCTA 
CGAATGATGG TGGCCAACAT TCTCAACAAC GAAGCCTTCA TGAATCAGGA GATCACCAAG 
GCCTTCCATG TACGCCGGAC CAACCGGGTT AAGGAGTGTT TGGCAAAGGG CACCTTTTCA 
GATGTGACCT TCATCCTGGA TGATGGGACC ATCAGCGCCC ATAAGCCCCT GCTGATTTCC 
AGCTGTGACT GGATGGCTGC CATGTTTGGG GGGCCATTTG TGGAGAGTTC TACCAGGGAG 
GTGGTGTTTC CATACACAAG CAAGAGCTGC ATGAGAGCTG TGTTGGAGTA CCTCTATACA 
GGCATGTTCA CCTCCAGCCC AGACCTGGAT GATATGAAGC TCATTGTCTT GGCTAACCGC 
CTCTGCCTGC CGCACTTGGT TGCCCTCACA GAGCAGTACA CAGTGACTGG ACTGATGGAG 
GCGACCCAGA TGATGGTGGA CATCGATGGG GATGTCCTTG TATTCTTGGA ATTGGCACAG 
TTCCACTGCG CGTACCAGTT GGCCGACTGG TGTCTTCACC ATATCTGCAC CAACTACAAC 
AACGTGTGCC GCAAGTTCCC CAGAGACATG AAGGCTATGT CTCCAGAAAA CCAGGAGTAC 
TTTGAGAAAC ACCGGTGGCC GCCAGTCTGG TACCTGAAAG AAGAGGACCA CTATCAGCGT 
GCGCGGAAGG AGCGAGAGAA AGAGGACTAT TTACACCTGC GGAGGCAGCC CAAGCGGCGG 
TGGCTGTTTT GGAACAGTCC CTCATCACCG TCCTCCTCAG CAGCGGGCTC AGCATCCCCC 
TCCTCCTCCT CCTCGGCTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705734.4
CDS	520..2706
Translation	MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSGSGGPRPEDHRSHPEQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTFKSRLMVVVKMDNSIQPGPFRAVLKYLYTGELGENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIVLANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLRRQPKRRWLFWNSPSSPSSSAAGSASPSSSSSAVV

Target ORF information:

RefSeq Version	NM_153514.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153514.5

ORF Insert Sequence:

ATGGATTCTG ACATGGATTA TGAAAGGCCA AACGTAGAGA CCATCAAGTG CGTGGTGGTG 
GGGGACAACG CCGTGGGCAA GACTAGGCTC ATCTGTGCCC GGGCCTGCAA TGCCACCCTC 
ACCCAGTACC AGTTGCTTGC CACCCATGTG CCCACAGTGT GGGCCATTGA CCAGTATCGT 
GTGTGCCAGG AGGTGCTAGA ACGCTCCCGA GATGTGGTAG ATGACGTCAG CGTCTCCCTG 
CGCCTCTGGG ACACCTTCGG AGACCACCAC AAAGACCGGC GTTTTGCTTA TGGAAGATCC 
GACGTGGTGG TTTTGTGTTT CTCCATCGCC AACCCCAATT CCCTCCACCA TGTCAAGACC 
ATGTGGTACC CAGAAATCAA GCATTTCTGC CCCAGAGCTC CTGTCATTCT GGTGGGCTGC 
CAGCTGGACC TGCGCTATGC TGACCTGGAA GCCGTTAACA GGGCCAGGAG ACCTCTGGCT 
AGGCCCATCA AGCCCAATGA AATCCTCCCC CCAGAGAAGG GTCGGGAAGT CGCCAAAGAG 
CTGGGGATCC CTTACTACGA GACCAGTGTG GTGGCCCAGT TTGGTATCAA GGACGTCTTT 
GACAATGCCA TTCGGGCTGC GCTCATCTCC CGCCGCCACC TGCAGTTCTG GAAATCACAT 
CTGCGCAATG TGCAGCGGCC TCTACTGCAA GCACCCTTCC TCCCCCCCAA GCCACCGCCC 
CCCATCATCG TGGTGCCCGA TCCCCCCTCC AGCAGCGAGG AGTGCCCCGC CCACCTCCTG 
GAAGACCCGC TCTGTGCGGA TGTCATCCTG GTGCTGCAGG AGCGCGTGCG CATCTTTGCC 
CACAAAATCT ACCTCTCCAC CTCCTCCTCC AAATTCTATG ACTTATTCCT CATGGACCTG 
AGTGAGGGGG AGCTGGGGGG CCCCTCAGGG TCAGGGGGGC CCCGCCCAGA GGACCACCGG 
AGCCACCCTG AACAACACCA CCATCATCAC CATCACCACC ATGGGCGGGA CTTCCTGCTT 
CGGGCAGCCA GCTTTGACGT ATGTGAGAGT GTGGATGAGG CCGGCGGCTC TGGTCCGGCT 
GGCCTAAGGG CTTCAACCAG TGACGGGATC TTACGGGGTA ATGGAACAGG GTACCTGCCA 
GGCAGAGGAC GTGTGCTGTC TTCCTGGAGC CGAGCTTTTG TGAGTATCCA GGAAGAGATG 
GCAGAGGATC CTCTGACCTT CAAATCCCGG CTGATGGTGG TGGTGAAGAT GGATAACTCC 
ATTCAGCCGG GGCCCTTCCG GGCTGTTCTC AAGTACCTGT ACACAGGGGA GCTAGGTGAG 
AATGAGCGGG ACCTCATGCA CATTGCTCAC ATTGCCGAGC TGCTAGAGGT CTTTGATCTA 
CGAATGATGG TGGCCAACAT TCTCAACAAC GAAGCCTTCA TGAATCAGGA GATCACCAAG 
GCCTTCCATG TACGCCGGAC CAACCGGGTT AAGGAGTGTT TGGCAAAGGG CACCTTTTCA 
GATGTGACCT TCATCCTGGA TGATGGGACC ATCAGCGCCC ATAAGCCCCT GCTGATTTCC 
AGCTGTGACT GGATGGCTGC CATGTTTGGG GGGCCATTTG TGGAGAGTTC TACCAGGGAG 
GTGGTGTTTC CATACACAAG CAAGAGCTGC ATGAGAGCTG TGTTGGAGTA CCTCTATACA 
GGCATGTTCA CCTCCAGCCC AGACCTGGAT GATATGAAGC TCATTGTCTT GGCTAACCGC 
CTCTGCCTGC CGCACTTGGT TGCCCTCACA GAGCAGTACA CAGTGACTGG ACTGATGGAG 
GCGACCCAGA TGATGGTGGA CATCGATGGG GATGTCCTTG TATTCTTGGA ATTGGCACAG 
TTCCACTGCG CGTACCAGTT GGCCGACTGG TGTCTTCACC ATATCTGCAC CAACTACAAC 
AACGTGTGCC GCAAGTTCCC CAGAGACATG AAGGCTATGT CTCCAGAAAA CCAGGAGTAC 
TTTGAGAAAC ACCGGTGGCC GCCAGTCTGG TACCTGAAAG AAGAGGACCA CTATCAGCGT 
GCGCGGAAGG AGCGAGAGAA AGAGGACTAT TTACACCTGC GGAGGCAGCC CAAGCGGCGG 
TGGCTGTTTT GGAACAGTCC CTCATCACCG TCCTCCTCAG CAGCGGGCTC AGCATCCCCC 
TCCTCCTCCT CCTCGGCTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhobtb2 ( XM_030247818.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103678.1 Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X2, mRNA.

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CloneID	OMu02518
Clone ID Related Accession (Same CDS sequence)	NM_153514.5 , XM_030247818.1
Accession Version	XM_030247818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	rho-related BTB domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACATGGATTA TGAAAGGCCA AACGTAGAGA CCATCAAGTG CGTGGTGGTG 
GGGGACAACG CCGTGGGCAA GACTAGGCTC ATCTGTGCCC GGGCCTGCAA TGCCACCCTC 
ACCCAGTACC AGTTGCTTGC CACCCATGTG CCCACAGTGT GGGCCATTGA CCAGTATCGT 
GTGTGCCAGG AGGTGCTAGA ACGCTCCCGA GATGTGGTAG ATGACGTCAG CGTCTCCCTG 
CGCCTCTGGG ACACCTTCGG AGACCACCAC AAAGACCGGC GTTTTGCTTA TGGAAGATCC 
GACGTGGTGG TTTTGTGTTT CTCCATCGCC AACCCCAATT CCCTCCACCA TGTCAAGACC 
ATGTGGTACC CAGAAATCAA GCATTTCTGC CCCAGAGCTC CTGTCATTCT GGTGGGCTGC 
CAGCTGGACC TGCGCTATGC TGACCTGGAA GCCGTTAACA GGGCCAGGAG ACCTCTGGCT 
AGGCCCATCA AGCCCAATGA AATCCTCCCC CCAGAGAAGG GTCGGGAAGT CGCCAAAGAG 
CTGGGGATCC CTTACTACGA GACCAGTGTG GTGGCCCAGT TTGGTATCAA GGACGTCTTT 
GACAATGCCA TTCGGGCTGC GCTCATCTCC CGCCGCCACC TGCAGTTCTG GAAATCACAT 
CTGCGCAATG TGCAGCGGCC TCTACTGCAA GCACCCTTCC TCCCCCCCAA GCCACCGCCC 
CCCATCATCG TGGTGCCCGA TCCCCCCTCC AGCAGCGAGG AGTGCCCCGC CCACCTCCTG 
GAAGACCCGC TCTGTGCGGA TGTCATCCTG GTGCTGCAGG AGCGCGTGCG CATCTTTGCC 
CACAAAATCT ACCTCTCCAC CTCCTCCTCC AAATTCTATG ACTTATTCCT CATGGACCTG 
AGTGAGGGGG AGCTGGGGGG CCCCTCAGGG TCAGGGGGGC CCCGCCCAGA GGACCACCGG 
AGCCACCCTG AACAACACCA CCATCATCAC CATCACCACC ATGGGCGGGA CTTCCTGCTT 
CGGGCAGCCA GCTTTGACGT ATGTGAGAGT GTGGATGAGG CCGGCGGCTC TGGTCCGGCT 
GGCCTAAGGG CTTCAACCAG TGACGGGATC TTACGGGGTA ATGGAACAGG GTACCTGCCA 
GGCAGAGGAC GTGTGCTGTC TTCCTGGAGC CGAGCTTTTG TGAGTATCCA GGAAGAGATG 
GCAGAGGATC CTCTGACCTT CAAATCCCGG CTGATGGTGG TGGTGAAGAT GGATAACTCC 
ATTCAGCCGG GGCCCTTCCG GGCTGTTCTC AAGTACCTGT ACACAGGGGA GCTAGGTGAG 
AATGAGCGGG ACCTCATGCA CATTGCTCAC ATTGCCGAGC TGCTAGAGGT CTTTGATCTA 
CGAATGATGG TGGCCAACAT TCTCAACAAC GAAGCCTTCA TGAATCAGGA GATCACCAAG 
GCCTTCCATG TACGCCGGAC CAACCGGGTT AAGGAGTGTT TGGCAAAGGG CACCTTTTCA 
GATGTGACCT TCATCCTGGA TGATGGGACC ATCAGCGCCC ATAAGCCCCT GCTGATTTCC 
AGCTGTGACT GGATGGCTGC CATGTTTGGG GGGCCATTTG TGGAGAGTTC TACCAGGGAG 
GTGGTGTTTC CATACACAAG CAAGAGCTGC ATGAGAGCTG TGTTGGAGTA CCTCTATACA 
GGCATGTTCA CCTCCAGCCC AGACCTGGAT GATATGAAGC TCATTGTCTT GGCTAACCGC 
CTCTGCCTGC CGCACTTGGT TGCCCTCACA GAGCAGTACA CAGTGACTGG ACTGATGGAG 
GCGACCCAGA TGATGGTGGA CATCGATGGG GATGTCCTTG TATTCTTGGA ATTGGCACAG 
TTCCACTGCG CGTACCAGTT GGCCGACTGG TGTCTTCACC ATATCTGCAC CAACTACAAC 
AACGTGTGCC GCAAGTTCCC CAGAGACATG AAGGCTATGT CTCCAGAAAA CCAGGAGTAC 
TTTGAGAAAC ACCGGTGGCC GCCAGTCTGG TACCTGAAAG AAGAGGACCA CTATCAGCGT 
GCGCGGAAGG AGCGAGAGAA AGAGGACTAT TTACACCTGC GGAGGCAGCC CAAGCGGCGG 
TGGCTGTTTT GGAACAGTCC CTCATCACCG TCCTCCTCAG CAGCGGGCTC AGCATCCCCC 
TCCTCCTCCT CCTCGGCTGT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103678.1
CDS	192..2378
Translation	MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSGSGGPRPEDHRSHPEQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTFKSRLMVVVKMDNSIQPGPFRAVLKYLYTGELGENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIVLANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLRRQPKRRWLFWNSPSSPSSSAAGSASPSSSSSAVV

Target ORF information:

RefSeq Version	XM_030247818.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-related BTB domain containing 2 (Rhobtb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247818.1

ORF Insert Sequence:

ATGGATTCTG ACATGGATTA TGAAAGGCCA AACGTAGAGA CCATCAAGTG CGTGGTGGTG 
GGGGACAACG CCGTGGGCAA GACTAGGCTC ATCTGTGCCC GGGCCTGCAA TGCCACCCTC 
ACCCAGTACC AGTTGCTTGC CACCCATGTG CCCACAGTGT GGGCCATTGA CCAGTATCGT 
GTGTGCCAGG AGGTGCTAGA ACGCTCCCGA GATGTGGTAG ATGACGTCAG CGTCTCCCTG 
CGCCTCTGGG ACACCTTCGG AGACCACCAC AAAGACCGGC GTTTTGCTTA TGGAAGATCC 
GACGTGGTGG TTTTGTGTTT CTCCATCGCC AACCCCAATT CCCTCCACCA TGTCAAGACC 
ATGTGGTACC CAGAAATCAA GCATTTCTGC CCCAGAGCTC CTGTCATTCT GGTGGGCTGC 
CAGCTGGACC TGCGCTATGC TGACCTGGAA GCCGTTAACA GGGCCAGGAG ACCTCTGGCT 
AGGCCCATCA AGCCCAATGA AATCCTCCCC CCAGAGAAGG GTCGGGAAGT CGCCAAAGAG 
CTGGGGATCC CTTACTACGA GACCAGTGTG GTGGCCCAGT TTGGTATCAA GGACGTCTTT 
GACAATGCCA TTCGGGCTGC GCTCATCTCC CGCCGCCACC TGCAGTTCTG GAAATCACAT 
CTGCGCAATG TGCAGCGGCC TCTACTGCAA GCACCCTTCC TCCCCCCCAA GCCACCGCCC 
CCCATCATCG TGGTGCCCGA TCCCCCCTCC AGCAGCGAGG AGTGCCCCGC CCACCTCCTG 
GAAGACCCGC TCTGTGCGGA TGTCATCCTG GTGCTGCAGG AGCGCGTGCG CATCTTTGCC 
CACAAAATCT ACCTCTCCAC CTCCTCCTCC AAATTCTATG ACTTATTCCT CATGGACCTG 
AGTGAGGGGG AGCTGGGGGG CCCCTCAGGG TCAGGGGGGC CCCGCCCAGA GGACCACCGG 
AGCCACCCTG AACAACACCA CCATCATCAC CATCACCACC ATGGGCGGGA CTTCCTGCTT 
CGGGCAGCCA GCTTTGACGT ATGTGAGAGT GTGGATGAGG CCGGCGGCTC TGGTCCGGCT 
GGCCTAAGGG CTTCAACCAG TGACGGGATC TTACGGGGTA ATGGAACAGG GTACCTGCCA 
GGCAGAGGAC GTGTGCTGTC TTCCTGGAGC CGAGCTTTTG TGAGTATCCA GGAAGAGATG 
GCAGAGGATC CTCTGACCTT CAAATCCCGG CTGATGGTGG TGGTGAAGAT GGATAACTCC 
ATTCAGCCGG GGCCCTTCCG GGCTGTTCTC AAGTACCTGT ACACAGGGGA GCTAGGTGAG 
AATGAGCGGG ACCTCATGCA CATTGCTCAC ATTGCCGAGC TGCTAGAGGT CTTTGATCTA 
CGAATGATGG TGGCCAACAT TCTCAACAAC GAAGCCTTCA TGAATCAGGA GATCACCAAG 
GCCTTCCATG TACGCCGGAC CAACCGGGTT AAGGAGTGTT TGGCAAAGGG CACCTTTTCA 
GATGTGACCT TCATCCTGGA TGATGGGACC ATCAGCGCCC ATAAGCCCCT GCTGATTTCC 
AGCTGTGACT GGATGGCTGC CATGTTTGGG GGGCCATTTG TGGAGAGTTC TACCAGGGAG 
GTGGTGTTTC CATACACAAG CAAGAGCTGC ATGAGAGCTG TGTTGGAGTA CCTCTATACA 
GGCATGTTCA CCTCCAGCCC AGACCTGGAT GATATGAAGC TCATTGTCTT GGCTAACCGC 
CTCTGCCTGC CGCACTTGGT TGCCCTCACA GAGCAGTACA CAGTGACTGG ACTGATGGAG 
GCGACCCAGA TGATGGTGGA CATCGATGGG GATGTCCTTG TATTCTTGGA ATTGGCACAG 
TTCCACTGCG CGTACCAGTT GGCCGACTGG TGTCTTCACC ATATCTGCAC CAACTACAAC 
AACGTGTGCC GCAAGTTCCC CAGAGACATG AAGGCTATGT CTCCAGAAAA CCAGGAGTAC 
TTTGAGAAAC ACCGGTGGCC GCCAGTCTGG TACCTGAAAG AAGAGGACCA CTATCAGCGT 
GCGCGGAAGG AGCGAGAGAA AGAGGACTAT TTACACCTGC GGAGGCAGCC CAAGCGGCGG 
TGGCTGTTTT GGAACAGTCC CTCATCACCG TCCTCCTCAG CAGCGGGCTC AGCATCCCCC 
TCCTCCTCCT CCTCGGCTGT GGTCTGA