Rasgrp3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrp3
Entrez Gene ID	240168
Full Name	RAS, guanyl releasing protein 3
Synonyms	BC066069,Gm327
General protein information	Preferred Names RAS, guanyl releasing protein 3 Names ras guanyl-releasing protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001360103.1	NP_001347032.1	ras guanyl-releasing protein 3
XM_006524273.3	XP_006524336.1	ras guanyl-releasing protein 3 isoform X2
NM_207246.4	NP_997129.1	ras guanyl-releasing protein 3
NM_001166493.1	NP_001159965.1	ras guanyl-releasing protein 3
XM_006524271.3	XP_006524334.1	ras guanyl-releasing protein 3 isoform X1
XM_006524272.3	XP_006524335.1	ras guanyl-releasing protein 3 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASGRP3	XP_005630385.1
Mus musculus (house mouse)	Rasgrp3	NP_001159965.1
Danio rerio (zebrafish)	rasgrp3	NP_001103934.1
Pan troglodytes (chimpanzee)	RASGRP3	XP_001165459.1
Caenorhabditis elegans (roundworm)	rgef-1	NP_505469.3
Rattus norvegicus (Norway rat)	Rasgrp3	NP_001101479.1
Gallus gallus (chicken)	RASGRP3	NP_001006401.1
Homo sapiens (human)	RASGRP3	NP_001132960.1
Macaca mulatta (Rhesus monkey)	RASGRP3	XP_001106737.1
Bos taurus (cattle)	RASGRP3	NP_001069316.1
Xenopus tropicalis (tropical clawed frog)	rasgrp3	XP_002934496.2

Gene Ontology
Bibliography

Related articles in PubMed

GNA11 Q209L Mouse Model Reveals RasGRP3 as an Essential Signaling Node in Uveal Melanoma.
Moore AR, Ran L, Guan Y, Sher JJ, Hitchman TD, Zhang JQ, Hwang C, Walzak EG, Shoushtari AN, Monette S, Murali R, Wiesner T, Griewank KG, Chi P, Chen Y
Cell reports22(9)2455-2468(2018 02)

RasGRP1 and RasGRP3 Are Required for Efficient Generation of Early Thymic Progenitors.
Golec DP, Henao Caviedes LM, Baldwin TA
Journal of immunology (Baltimore, Md. : 1950)197(5)1743-53(2016 09)

RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase.
Tang S, Chen T, Yu Z, Zhu X, Yang M, Xie B, Li N, Cao X, Wang J
Nature communications54657(2014 Aug)

RasGRP1, but not RasGRP3, is required for efficient thymic β-selection and ERK activation downstream of CXCR4.
Golec DP, Dower NA, Stone JC, Baldwin TA
PloS one8(1)e53300(2013)

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.
Costello I, Pimeisl IM, Dräger S, Bikoff EK, Robertson EJ, Arnold SJ
Nature cell biology13(9)1084-91(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasgrp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09904	NM_001360103.1 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09904	NM_207246.4 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09904	NM_001166493.1 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09904	XM_006524272.3 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09904	XM_006524271.3 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu53584	XM_006524273.3 Latest version!	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrp3 ( NM_001360103.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347032.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu09904
Clone ID Related Accession (Same CDS sequence)	NM_001360103.1 , NM_207246.4 , NM_001166493.1 , XM_006524271.3 , XM_006524272.3
Accession Version	NM_001360103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571846400000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010501.6. On Jan 27, 2018 this sequence version replaced XM_006524272.3. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.68840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347032.1
CDS	257..2332
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	NM_001360103.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360103.1

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrp3 ( NM_207246.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997129.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09904
Clone ID Related Accession (Same CDS sequence)	NM_001360103.1 , NM_207246.4 , NM_001166493.1 , XM_006524271.3 , XM_006524272.3
Accession Version	NM_207246.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561392000000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ131259.1, BY123705.1 and BC066069.1. On Oct 27, 2009 this sequence version replaced NM_207246.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997129.1
CDS	390..2465
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	NM_207246.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207246.4

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrp3 ( NM_001166493.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159965.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09904
Clone ID Related Accession (Same CDS sequence)	NM_001360103.1 , NM_207246.4 , NM_001166493.1 , XM_006524271.3 , XM_006524272.3
Accession Version	NM_001166493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561392000000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ064681.1, CN695386.1, CD348455.1 and BC066069.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173041.1, SRR1660821.162043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159965.1
CDS	398..2473
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	NM_001166493.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166493.1

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrp3 ( XM_006524271.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524334.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09904
Clone ID Related Accession (Same CDS sequence)	NM_001360103.1 , NM_207246.4 , NM_001166493.1 , XM_006524271.3 , XM_006524272.3
Accession Version	XM_006524271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524334.1
CDS	565..2640
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	XM_006524271.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524271.3

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrp3 ( XM_006524272.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524335.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu09904
Clone ID Related Accession (Same CDS sequence)	NM_001360103.1 , NM_207246.4 , NM_001166493.1 , XM_006524271.3 , XM_006524272.3
Accession Version	XM_006524272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524272.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524335.1
CDS	463..2538
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	XM_006524272.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524272.3

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCAGCCAACC TCCCCAACGA CACCTAATAA GCCTGTGGTA 
CCTCTGGAGT GGGCTTCAGA AGTGGTACCA AAGCCAGACC CCACAATCAT CAACAAACAC 
ATAAGGAAGT TGGTTGAGTC TGTGTTTCGA AACTACGATC ATGACCACGA TGGTTACATC 
TCCCAAGAGG ACTTTGAGAG CATAGCTGCC AATTTCCCCT TCCTGGATTC CTTCTGCGTA 
CTAGACAAAG ATCAGGACGG CCTAATTAGC AAAGACGAAA TGATGGCTTA TTTCCTGAGA 
GCTAAATCTC AGCTACACTG CAAAATGGGA CCAGGCTTCA TCCATAATTT TCAGGAGATG 
AACTATCTCA AGCCGACCTT CTGCGAACAC TGTGCGGGAT TTCTCTGGGG CATAATCAAA 
CAAGGCTACA AGTGCAAAGA CTGTGGAGCC AACTGCCACA AGCAGTGCAA AGACCTCTTG 
GTTCTGGCCT GCAGGAGACT TGCCAGGGCA CCCTCCTTGA GCAGCAACCC CGGGTCACTG 
CCTGGAAGCC CAGCCTTGCC CCCAGTGCAG GATGAGGTAT TTGAGTTTCC TGGGGTCACG 
GCTGGACACC GAGACCTGGA CAGCAGAGCC ATTACCCTGG TTACAGGATC TTCCCGGAAG 
ATCTCCGTGA GGCTGCAGAG AGCCACCACT AGTCAAGCCA CCCAGACGGA ACCTGTGTGG 
TCAGAGGCTG TCTGGGGAGA CTCAGGGTCC CATACCTTCC CCAAAATGAA ATCCAAGTTC 
CATGACAAAG CAGCAAAGGA CAAGGGCTTT GCCAAGTGGG AAAACGAGAA GCCCACGGTA 
CAAGCTGGTG TGGATGTTGT AGATCGAGGC ACTGCATTTG AACCTGACCA GGATGATGGG 
CAAGATGAAG CCAAGCAGGG TGGTGAGGAT GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrp3 ( XM_006524273.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524336.1 Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.

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CloneID	OMu53584
Clone ID Related Accession (Same CDS sequence)	XM_006524273.3
Accession Version	XM_006524273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ras guanyl-releasing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCCAACCTCC CCAACGACAC CTAATAAGCC TGTGGTACCT 
CTGGAGTGGG CTTCAGAAGT GGTACCAAAG CCAGACCCCA CAATCATCAA CAAACACATA 
AGGAAGTTGG TTGAGTCTGT GTTTCGAAAC TACGATCATG ACCACGATGG TTACATCTCC 
CAAGAGGACT TTGAGAGCAT AGCTGCCAAT TTCCCCTTCC TGGATTCCTT CTGCGTACTA 
GACAAAGATC AGGACGGCCT AATTAGCAAA GACGAAATGA TGGCTTATTT CCTGAGAGCT 
AAATCTCAGC TACACTGCAA AATGGGACCA GGCTTCATCC ATAATTTTCA GGAGATGAAC 
TATCTCAAGC CGACCTTCTG CGAACACTGT GCGGGATTTC TCTGGGGCAT AATCAAACAA 
GGCTACAAGT GCAAAGACTG TGGAGCCAAC TGCCACAAGC AGTGCAAAGA CCTCTTGGTT 
CTGGCCTGCA GGAGACTTGC CAGGGCACCC TCCTTGAGCA GCAACCCCGG GTCACTGCCT 
GGAAGCCCAG CCTTGCCCCC AGTGCAGGAT GAGGTATTTG AGTTTCCTGG GGTCACGGCT 
GGACACCGAG ACCTGGACAG CAGAGCCATT ACCCTGGTTA CAGGATCTTC CCGGAAGATC 
TCCGTGAGGC TGCAGAGAGC CACCACTAGT CAAGCCACCC AGACGGAACC TGTGTGGTCA 
GAGGCTGTCT GGGGAGACTC AGGGTCCCAT ACCTTCCCCA AAATGAAATC CAAGTTCCAT 
GACAAAGCAG CAAAGGACAA GGGCTTTGCC AAGTGGGAAA ACGAGAAGCC CACGGTACAA 
GCTGGTGTGG ATGTTGTAGA TCGAGGCACT GCATTTGAAC CTGACCAGGA TGATGGGCAA 
GATGAAGCCA AGCAGGGTGG TGAGGATGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524336.1
CDS	566..2638
Translation	MGSNGLGKAATLDELLSTCIEMFDDNGDLNDSYLPRIVLLMHRWYLSSTELAGKLLCMYRNASGESCDEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGHEKHISLMDISSVPSYDWMRRVTQRKKVSKRGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSPTSPTTPNKPVVPLEWASEVVPKPDPTIINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMNYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPGSLPGSPALPPVQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPTVQAGVDVVDRGTAFEPDQDDGQDEAKQGGEDG

Target ORF information:

RefSeq Version	XM_006524273.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS, guanyl releasing protein 3 (Rasgrp3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524273.3

ORF Insert Sequence:

ATGGGGTCCA ACGGGCTCGG GAAAGCGGCA ACACTAGATG AACTTCTCAG CACATGCATT 
GAGATGTTTG ATGACAATGG AGACCTGAAC GACAGTTACT TGCCCAGAAT CGTGTTACTG 
ATGCACCGAT GGTATTTATC CTCCACTGAA CTGGCAGGAA AACTCCTCTG CATGTATCGG 
AATGCCAGTG GAGAAAGCTG TGATGAGTTC CGACTGAAGA TCTGCTATTT CATGAGGTAC 
TGGATTCTGA AATTTCCTGC AGAGTTTAAT TTGGATCTTG GTTTGATTCG CATGACTGAG 
GAGTTCCGGG AAGTAGCTAG TCAACTAGGA CATGAAAAAC ACATCAGCCT CATGGACATA 
TCTAGCGTTC CTTCCTATGA CTGGATGAGG AGAGTCACAC AGAGGAAAAA AGTATCCAAA 
AGGGGAAAGG CCTGCCTCTT ATTTGACCAT CTGGAGCCCA TTGAGTTGGC GGAGCACCTC 
ACTTTTCTGG AGCATAAATC TTTTAGAAGG ATCTCATTCA CAGATTATCA GAGCTATGTC 
ATCCATGGCT GTCTGGAGAA TAACCCGACA TTGGAGAGAT CTATTGCTTT ATTTAATGGA 
ATCTCTAAGT GGGTCCAGTT GATGGTTCTT AGCAAACCAA GTGCGCAACA AAGGGCAGAA 
GTCATCACAA AGTTCATCAA TGTAGCCCAG AAACTCCTTC AACTAAAAAA TTTTAACACC 
TTGATGGCAG TGGTTGGTGG CCTTAGTCAC AGCTCCATTT CCCGACTCAA AGACACCCAT 
TCTCACCTCT CTTCCGAAGT TACAAAGAAC TGGAATGAAA TGACAGAGTT GGTCTCCTCC 
AATGGCAATT ACTGCAATTA CCGCAAAGCC TTTGCTGACT GTGATGGCTT CAAAATCCCC 
ATCCTTGGCG TGCACTTGAA AGACTTGATA GCAGTCCATG TCATTTTCCC AGACTGGATG 
GAGGAGAACA AAGTGAACGT TGTGAAAATG CACCAGCTTT CTGTTACCCT GAGTGAACTG 
GTCTCCCTGC AGAATGCCTC TCACCACTTG GAACCTAACA TGGATTTGAT CAACCTACTC 
ACTCTCTCTC TGGACCTCTA CCACACAGAG GATGATATTT ACAAACTGTC ACTGGTGTTG 
GAGCCTAGAA ATTCCAAATC GCCAACCTCC CCAACGACAC CTAATAAGCC TGTGGTACCT 
CTGGAGTGGG CTTCAGAAGT GGTACCAAAG CCAGACCCCA CAATCATCAA CAAACACATA 
AGGAAGTTGG TTGAGTCTGT GTTTCGAAAC TACGATCATG ACCACGATGG TTACATCTCC 
CAAGAGGACT TTGAGAGCAT AGCTGCCAAT TTCCCCTTCC TGGATTCCTT CTGCGTACTA 
GACAAAGATC AGGACGGCCT AATTAGCAAA GACGAAATGA TGGCTTATTT CCTGAGAGCT 
AAATCTCAGC TACACTGCAA AATGGGACCA GGCTTCATCC ATAATTTTCA GGAGATGAAC 
TATCTCAAGC CGACCTTCTG CGAACACTGT GCGGGATTTC TCTGGGGCAT AATCAAACAA 
GGCTACAAGT GCAAAGACTG TGGAGCCAAC TGCCACAAGC AGTGCAAAGA CCTCTTGGTT 
CTGGCCTGCA GGAGACTTGC CAGGGCACCC TCCTTGAGCA GCAACCCCGG GTCACTGCCT 
GGAAGCCCAG CCTTGCCCCC AGTGCAGGAT GAGGTATTTG AGTTTCCTGG GGTCACGGCT 
GGACACCGAG ACCTGGACAG CAGAGCCATT ACCCTGGTTA CAGGATCTTC CCGGAAGATC 
TCCGTGAGGC TGCAGAGAGC CACCACTAGT CAAGCCACCC AGACGGAACC TGTGTGGTCA 
GAGGCTGTCT GGGGAGACTC AGGGTCCCAT ACCTTCCCCA AAATGAAATC CAAGTTCCAT 
GACAAAGCAG CAAAGGACAA GGGCTTTGCC AAGTGGGAAA ACGAGAAGCC CACGGTACAA 
GCTGGTGTGG ATGTTGTAGA TCGAGGCACT GCATTTGAAC CTGACCAGGA TGATGGGCAA 
GATGAAGCCA AGCAGGGTGG TGAGGATGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GNA11 Q209L Mouse Model Reveals RasGRP3 as an Essential Signaling Node in Uveal Melanoma.
Cell reports22(9)2455-2468(2018 02)
Moore AR,Ran L,Guan Y,Sher JJ,Hitchman TD,Zhang JQ,Hwang C,Walzak EG,Shoushtari AN,Monette S,Murali R,Wiesner T,Griewank KG,Chi P,Chen Y

RasGRP1 and RasGRP3 Are Required for Efficient Generation of Early Thymic Progenitors.
Journal of immunology (Baltimore, Md. : 1950)197(5)1743-53(2016 09)
Golec DP,Henao Caviedes LM,Baldwin TA

RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase.
Nature communications54657(2014 Aug)
Tang S,Chen T,Yu Z,Zhu X,Yang M,Xie B,Li N,Cao X,Wang J

RasGRP1, but not RasGRP3, is required for efficient thymic β-selection and ERK activation downstream of CXCR4.
PloS one8(1)e53300(2013)
Golec DP,Dower NA,Stone JC,Baldwin TA

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.
Nature cell biology13(9)1084-91(2011 Aug)
Costello I,Pimeisl IM,Dräger S,Bikoff EK,Robertson EJ,Arnold SJ

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Rasgrp3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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