Chmp1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chmp1a
Entrez Gene ID	234852
Full Name	charged multivesicular body protein 1A
Synonyms	2900018H07Rik,Pcoln3
General protein information	Preferred Names charged multivesicular body protein 1A Names charged multivesicular body protein 1a chromatin modifying protein 1A chromatin-modifying protein 1a procollagen (type III) N-endopeptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145606.3	NP_663581.1	charged multivesicular body protein 1a

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CHMP1A	NP_001078827.1
Bos taurus (cattle)	CHMP1A	NP_001178189.1
Homo sapiens (human)	CHMP1A	NP_002759.2
Canis lupus familiaris (dog)	CHMP1A	XP_546776.2
Danio rerio (zebrafish)	chmp1a	NP_956857.1
Gallus gallus (chicken)	CHMP1A	NP_001020611.1
Xenopus tropicalis (tropical clawed frog)	chmp1a	NP_001135608.1
Rattus norvegicus (Norway rat)	Chmp1a	NP_001076782.1
Mus musculus (house mouse)	Chmp1a	NP_663581.1

Gene Ontology
Bibliography

Related articles in PubMed

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Cell reports24(4)973-986.e8(2018 07)

Germline Chd8 haploinsufficiency alters brain development in mouse.
Gompers AL, Su-Feher L, Ellegood J, Copping NA, Riyadh MA, Stradleigh TW, Pride MC, Schaffler MD, Wade AA, Catta-Preta R, Zdilar I, Louis S, Kaushik G, Mannion BJ, Plajzer-Frick I, Afzal V, Visel A, Pennacchio LA, Dickel DE, Lerch JP, Crawley JN, Zarbalis KS, Silverman JL, Nord AS
Nature neuroscience20(8)1062-1073(2017 Aug)

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chmp1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chmp1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11877	NM_145606.3 Latest version!	Mus musculus charged multivesicular body protein 1A (Chmp1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chmp1a ( NM_145606.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663581.1 Mus musculus charged multivesicular body protein 1A (Chmp1a), mRNA.

Price & Availability↑

CloneID	OMu11877
Clone ID Related Accession (Same CDS sequence)	NM_145606.3
Accession Version	NM_145606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Mus musculus(house mouse)
Product	charged multivesicular body protein 1a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY112937.1, AK159830.1 and CJ325786.1. On Apr 16, 2009 this sequence version replaced NM_145606.2. ##Evidence-Data-START## Transcript exon combination :: BC036152.1, SRR1660819.8669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374 [ECO:0000348] ##Evidence-Data-END##

ATGGACGATA CCCTGTTCCA GTTGAAGTTC ACAGCGAAGC AGCTGGAGAA ACTGGCCAAG 
AAGGCCGAGA AGGACTCCAA GGCTGAGCAG GCCAAAGTGA AGAAGGCCCT TCAGCAGAAA 
AATGTGGAGT GTGCCCGGGT GTATGCTGAA AATGCCATCC GCAAGAAGAA TGAAGGTGTA 
AATTGGCTCC GAATGGCCTC CCGTGTGGAC GCAGTGGCCT CCAAGGTGCA GACAGCTGTG 
ACCATGAAGG GGGTGACCAA GAATATGGCC CAGGTGACCA AGGCTCTGGA CAAGGCCCTG 
AGCGCCATGG ACCTTCAGAA GGTGTCTGCA GTGATGGACA GGTTTGAGCA GCAGGTGCAG 
AACCTGGATG TGCACACATC GGTAATGGAG GATTCCGTGA GCTCTGCCAC CACGCTGACC 
ACGCCTCAGG AGCAGGTCGA CAGCCTCATT GTCCAGATAG CTGAGGAAAA CGGCCTGGAG 
GTCCTAGACC AGCTCAGCCA GCTGCCAGAG GGAGCGTCTG CTGTGGGCGA GAGCTCTGTG 
CGCAGCCAGG AGGACCAGCT GTCCCGGAGG TTGGCTGCCC TGAGGAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663581.1
CDS	117..707
Translation	MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALQQKNVECARVYAENAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDKALSAMDLQKVSAVMDRFEQQVQNLDVHTSVMEDSVSSATTLTTPQEQVDSLIVQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRLAALRN

Target ORF information:

RefSeq Version	NM_145606.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus charged multivesicular body protein 1A (Chmp1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145606.3

ORF Insert Sequence:

ATGGACGATA CCCTGTTCCA GTTGAAGTTC ACAGCGAAGC AGCTGGAGAA ACTGGCCAAG 
AAGGCCGAGA AGGACTCCAA GGCTGAGCAG GCCAAAGTGA AGAAGGCCCT TCAGCAGAAA 
AATGTGGAGT GTGCCCGGGT GTATGCTGAA AATGCCATCC GCAAGAAGAA TGAAGGTGTA 
AATTGGCTCC GAATGGCCTC CCGTGTGGAC GCAGTGGCCT CCAAGGTGCA GACAGCTGTG 
ACCATGAAGG GGGTGACCAA GAATATGGCC CAGGTGACCA AGGCTCTGGA CAAGGCCCTG 
AGCGCCATGG ACCTTCAGAA GGTGTCTGCA GTGATGGACA GGTTTGAGCA GCAGGTGCAG 
AACCTGGATG TGCACACATC GGTAATGGAG GATTCCGTGA GCTCTGCCAC CACGCTGACC 
ACGCCTCAGG AGCAGGTCGA CAGCCTCATT GTCCAGATAG CTGAGGAAAA CGGCCTGGAG 
GTCCTAGACC AGCTCAGCCA GCTGCCAGAG GGAGCGTCTG CTGTGGGCGA GAGCTCTGTG 
CGCAGCCAGG AGGACCAGCT GTCCCGGAGG TTGGCTGCCC TGAGGAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
Cell reports24(4)973-986.e8(2018 07)
Coulter ME,Dorobantu CM,Lodewijk GA,Delalande F,Cianferani S,Ganesh VS,Smith RS,Lim ET,Xu CS,Pang S,Wong ET,Lidov HGW,Calicchio ML,Yang E,Gonzalez DM,Schlaeger TM,Mochida GH,Hess H,Lee WA,Lehtinen MK,Kirchhausen T,Haussler D,Jacobs FMJ,Gaudin R,Walsh CA

Germline Chd8 haploinsufficiency alters brain development in mouse.
Nature neuroscience20(8)1062-1073(2017 Aug)
Gompers AL,Su-Feher L,Ellegood J,Copping NA,Riyadh MA,Stradleigh TW,Pride MC,Schaffler MD,Wade AA,Catta-Preta R,Zdilar I,Louis S,Kaushik G,Mannion BJ,Plajzer-Frick I,Afzal V,Visel A,Pennacchio LA,Dickel DE,Lerch JP,Crawley JN,Zarbalis KS,Silverman JL,Nord AS

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Chmp1a cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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