Kif18a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif18a
Entrez Gene ID	228421
Full Name	kinesin family member 18A
Synonyms	AU024633,B130001M12Rik,gcd2
General protein information	Preferred Names kinesin family member 18A Names kinesin-like protein KIF18A N-8 kinesin kinesin superfamily protein 18A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499259.1	XP_006499322.1	kinesin-like protein KIF18A isoform X1
XM_006499259.2	XP_006499322.1	kinesin-like protein KIF18A isoform X1
NM_139303.1	NP_647464.1	kinesin-like protein KIF18A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Kif18a	NP_647464.1
Xenopus tropicalis (tropical clawed frog)	kif18a	NP_001016245.2
Homo sapiens (human)	KIF18A	NP_112494.3
Canis lupus familiaris (dog)	KIF18A	XP_005633821.1
Bos taurus (cattle)	KIF18A	NP_001179838.1
Danio rerio (zebrafish)	kif18a	NP_956533.1
Pan troglodytes (chimpanzee)	KIF18A	XP_001138250.1
Macaca mulatta (Rhesus monkey)	KIF18A	XP_001089262.1
Rattus norvegicus (Norway rat)	Kif18a	NP_001131114.1
Gallus gallus (chicken)	KIF18A	NP_001186355.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase.
Fonseca CL, Malaby HLH, Sepaniac LA, Martin W, Byers C, Czechanski A, Messinger D, Tang M, Ohi R, Reinholdt LG, Stumpff J
The Journal of cell biology218(4)1148-1163(2019 Apr)

Kif18a is specifically required for mitotic progression during germ line development.
Czechanski A, Kim H, Byers C, Greenstein I, Stumpff J, Reinholdt LG
Developmental biology402(2)253-262(2015 Jun)

Targeted deletion of Kif18a protects from colitis-associated colorectal (CAC) tumors in mice through impairing Akt phosphorylation.
Zhu H, Xu W, Zhang H, Liu J, Xu H, Lu S, Dang S, Kuang Y, Jin X, Wang Z
Biochemical and biophysical research communications438(1)97-102(2013 Aug)

Germinal Cell Aplasia in Kif18a Mutant Male Mice Due to Impaired Chromosome Congression and Dysregulated BubR1 and CENP-E.
Liu XS, Zhao XD, Wang X, Yao YX, Zhang LL, Shu RZ, Ren WH, Huang Y, Huang L, Gu MM, Kuang Y, Wang L, Lu SY, Chi J, Fen JS, Wang YF, Fei J, Dai W, Wang ZG
Genes & cancer1(1)26-39(2010 Jan)

Analysis of Pax6 contiguous gene deletions in the mouse, Mus musculus, identifies regions distinct from Pax6 responsible for extreme small-eye and belly-spotting phenotypes.
Favor J, Bradley A, Conte N, Janik D, Pretsch W, Reitmeir P, Rosemann M, Schmahl W, Wienberg J, Zaus I
Genetics182(4)1077-88(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kif18a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif18a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30304	XM_006499259.2 Latest version!	Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu11017	NM_139303.1 Latest version!	Mus musculus kinesin family member 18A (Kif18a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$517.30 $739.00
View Old Accession Versions >>
OMu30304	XM_006499259.1	Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif18a ( XM_006499259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499322.1 Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30304
Clone ID Related Accession (Same CDS sequence)	XM_006499259.1 , XM_006499259.2
Accession Version	XM_006499259.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF18A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGCTATA 
AAAGAAATAC CAACAAAACA AATTCAAACA TGCTTAGAAA ATTTAGAAGA AACACTTCAA 
AAGAAAATGT ACAATAAGAA GAATCAATGT CCTTGTGTGG TATTTATGCC TTTAGAAATG 
TATTTCAAGT CTGGGAAAGA AGGCCATCTT TTGCTCAAAT ATTTTCAGCA AGCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499322.1
CDS	195..2954
Translation	MSGTEEDLCHRMKVVVRVRPENTKEKAVQFCKVVHVVDKHILSFDPKQEEISFFHRKKTTNFDITKRQNKDLKFVFDAVFDETSTQMEVFEHTTKPILHSFLNGYNCTVFAYGATGSGKTHTMLGSAAEPGVMYLTMLDLFKCIDEIKEEKECSTAVSYLEVYNEQIRDLLTNSGPLAVREDSQKGVVVQGLTLHQPKSSEEILQLLDNGNKNRTQHPTDVNAVSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASVSGAKGSRFVEGTNINKSLLALGNVINALANTKRRNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSLFYDDTYNTLKYANRAKEIKSSLKSNVLNLNSHISQYVKICNMQKAEILMLKEKLKAYEEQKALSDRNDCAKLVHSNPEDRETERFQEILNCLFQNREGIRQEYLKLEMLLKANALKSSYHQQCHKQIEMMCSEDKVEKATCKRDHRLEKLKTNSCFLEKKKEEVSKQFDENTNWLHRVENEMRLLGQNGDIPEALNKELHCHHLHLQNKELKTQMAHMTALACLQEQQHKQTEAVLNALLPVLRKQYWKLKETGLSNAAFDSDFKDIEHLVERKKVVAWADQTNEHSNRNDLPGISLLMTFPQLEPIQSISCCTSVSDPNVLKLTPQRRTRRKIIPSPLKVQHTQKSALSESTQLNDSFSKELQPIVYTPEDCKKAQDLFPSLTRTSSQSANVMNDNSQKALCRIESPLSRTECKQGLYSTSTLCDSIRGLKNKWPEQEPLASSKSSVHRIESSSFSTKDSMPESAGVPSYMAMTTAAKRKWKQMSSTSNASIKSDESCGFAKRIRRDNSSVKPMQENRLKAIKEIPTKQIQTCLENLEETLQKKMYNKKNQCPCVVFMPLEMYFKSGKEGHLLLKYFQQAK

Target ORF information:

RefSeq Version	XM_006499259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499259.1

ORF Insert Sequence:

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGCTATA 
AAAGAAATAC CAACAAAACA AATTCAAACA TGCTTAGAAA ATTTAGAAGA AACACTTCAA 
AAGAAAATGT ACAATAAGAA GAATCAATGT CCTTGTGTGG TATTTATGCC TTTAGAAATG 
TATTTCAAGT CTGGGAAAGA AGGCCATCTT TTGCTCAAAT ATTTTCAGCA AGCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif18a ( XM_006499259.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499322.1 Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30304
Clone ID Related Accession (Same CDS sequence)	XM_006499259.1 , XM_006499259.2
Accession Version	XM_006499259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF18A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGCTATA 
AAAGAAATAC CAACAAAACA AATTCAAACA TGCTTAGAAA ATTTAGAAGA AACACTTCAA 
AAGAAAATGT ACAATAAGAA GAATCAATGT CCTTGTGTGG TATTTATGCC TTTAGAAATG 
TATTTCAAGT CTGGGAAAGA AGGCCATCTT TTGCTCAAAT ATTTTCAGCA AGCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499322.1
CDS	150..2909
Translation	MSGTEEDLCHRMKVVVRVRPENTKEKAVQFCKVVHVVDKHILSFDPKQEEISFFHRKKTTNFDITKRQNKDLKFVFDAVFDETSTQMEVFEHTTKPILHSFLNGYNCTVFAYGATGSGKTHTMLGSAAEPGVMYLTMLDLFKCIDEIKEEKECSTAVSYLEVYNEQIRDLLTNSGPLAVREDSQKGVVVQGLTLHQPKSSEEILQLLDNGNKNRTQHPTDVNAVSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASVSGAKGSRFVEGTNINKSLLALGNVINALANTKRRNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSLFYDDTYNTLKYANRAKEIKSSLKSNVLNLNSHISQYVKICNMQKAEILMLKEKLKAYEEQKALSDRNDCAKLVHSNPEDRETERFQEILNCLFQNREGIRQEYLKLEMLLKANALKSSYHQQCHKQIEMMCSEDKVEKATCKRDHRLEKLKTNSCFLEKKKEEVSKQFDENTNWLHRVENEMRLLGQNGDIPEALNKELHCHHLHLQNKELKTQMAHMTALACLQEQQHKQTEAVLNALLPVLRKQYWKLKETGLSNAAFDSDFKDIEHLVERKKVVAWADQTNEHSNRNDLPGISLLMTFPQLEPIQSISCCTSVSDPNVLKLTPQRRTRRKIIPSPLKVQHTQKSALSESTQLNDSFSKELQPIVYTPEDCKKAQDLFPSLTRTSSQSANVMNDNSQKALCRIESPLSRTECKQGLYSTSTLCDSIRGLKNKWPEQEPLASSKSSVHRIESSSFSTKDSMPESAGVPSYMAMTTAAKRKWKQMSSTSNASIKSDESCGFAKRIRRDNSSVKPMQENRLKAIKEIPTKQIQTCLENLEETLQKKMYNKKNQCPCVVFMPLEMYFKSGKEGHLLLKYFQQAK

Target ORF information:

RefSeq Version	XM_006499259.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 18A (Kif18a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499259.2

ORF Insert Sequence:

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGCTATA 
AAAGAAATAC CAACAAAACA AATTCAAACA TGCTTAGAAA ATTTAGAAGA AACACTTCAA 
AAGAAAATGT ACAATAAGAA GAATCAATGT CCTTGTGTGG TATTTATGCC TTTAGAAATG 
TATTTCAAGT CTGGGAAAGA AGGCCATCTT TTGCTCAAAT ATTTTCAGCA AGCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif18a ( NM_139303.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_647464.1 Mus musculus kinesin family member 18A (Kif18a), mRNA.

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CloneID	OMu11017
Clone ID Related Accession (Same CDS sequence)	NM_139303.1
Accession Version	NM_139303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF18A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016095.1. ##Evidence-Data-START## Transcript exon combination :: BC016095.1, AK049197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGTGGGC 
TATAAAAGAA ATACCAACAA AACAAATTCA AACATGCTTA GAAAATTTAG AAGAAACACT 
TCAAAAGAAA ATGTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647464.1
CDS	202..2862
Translation	MSGTEEDLCHRMKVVVRVRPENTKEKAVQFCKVVHVVDKHILSFDPKQEEISFFHRKKTTNFDITKRQNKDLKFVFDAVFDETSTQMEVFEHTTKPILHSFLNGYNCTVFAYGATGSGKTHTMLGSAAEPGVMYLTMLDLFKCIDEIKEEKECSTAVSYLEVYNEQIRDLLTNSGPLAVREDSQKGVVVQGLTLHQPKSSEEILQLLDNGNKNRTQHPTDVNAVSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASVSGAKGSRFVEGTNINKSLLALGNVINALANTKRRNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSLFYDDTYNTLKYANRAKEIKSSLKSNVLNLNSHISQYVKICNMQKAEILMLKEKLKAYEEQKALSDRNDCAKLVHSNPEDRETERFQEILNCLFQNREGIRQEYLKLEMLLKANALKSSYHQQCHKQIEMMCSEDKVEKATCKRDHRLEKLKTNSCFLEKKKEEVSKQFDENTNWLHRVENEMRLLGQNGDIPEALNKELHCHHLHLQNKELKTQMAHMTALACLQEQQHKQTEAVLNALLPVLRKQYWKLKETGLSNAAFDSDFKDIEHLVERKKVVAWADQTNEHSNRNDLPGISLLMTFPQLEPIQSISCCTSVSDPNVLKLTPQRRTRRKIIPSPLKVQHTQKSALSESTQLNDSFSKELQPIVYTPEDCKKAQDLFPSLTRTSSQSANVMNDNSQKALCRIESPLSRTECKQGLYSTSTLCDSIRGLKNKWPEQEPLASSKSSVHRIESSSFSTKDSMPESAGVPSYMAMTTAAKRKWKQMSSTSNASIKSDESCGFAKRIRRDNSSVKPMQENRLKVGYKRNTNKTNSNMLRKFRRNTSKENVQ

Target ORF information:

RefSeq Version	NM_139303.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 18A (Kif18a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139303.1

ORF Insert Sequence:

ATGTCTGGCA CTGAAGAAGA CTTATGTCAC CGCATGAAAG TAGTTGTCCG TGTACGTCCT 
GAGAACACAA AAGAAAAGGC GGTGCAGTTC TGTAAAGTGG TTCATGTAGT GGATAAACAT 
ATACTCAGTT TTGATCCGAA ACAAGAAGAA ATCAGTTTTT TTCACAGAAA GAAAACTACA 
AATTTTGATA TTACTAAAAG GCAAAATAAA GATCTGAAGT TTGTATTTGA TGCTGTCTTT 
GATGAAACTT CAACTCAAAT GGAAGTTTTT GAACACACAA CCAAGCCAAT CCTTCATAGT 
TTTCTGAATG GATACAATTG CACAGTATTT GCATATGGTG CCACTGGATC TGGGAAAACT 
CACACAATGC TAGGATCAGC TGCTGAACCT GGGGTCATGT ACCTGACAAT GCTGGACCTT 
TTCAAATGCA TCGATGAGAT TAAAGAAGAG AAAGAGTGTA GCACTGCCGT GTCATACCTG 
GAGGTATATA ATGAACAGAT CCGTGACCTC TTGACAAACT CAGGACCACT TGCTGTCCGG 
GAAGATTCCC AAAAAGGAGT GGTTGTTCAG GGTCTTACTC TACACCAGCC TAAATCTTCA 
GAGGAAATAT TACAGCTACT AGACAATGGA AACAAAAACA GGACCCAGCA TCCCACTGAC 
GTGAATGCAG TGTCTTCTCG TTCCCATGCT GTTTTCCAGA TTTATTTGCG ACAACAAGAC 
AAAACAGCGA GCATCAATCA AAATGTCCGT ATAGCCAAAA TGTCACTCAT CGACCTCGCA 
GGATCTGAGA GGGCCAGTGT TTCGGGTGCT AAAGGGTCAC GATTTGTAGA AGGCACAAAT 
ATTAACAAAT CTCTTTTAGC TCTTGGGAAT GTCATCAATG CCTTAGCAAA TACAAAGAGA 
AGAAATCAGC ATATTCCTTA CAGAAATAGT AAGCTTACTC GTTTGCTGAA GGATTCTCTT 
GGAGGAAACT GTCAAACTAT AATGATAGCT GCTGTTAGTC CTTCCTCTTT ATTTTATGAT 
GACACATATA ACACTCTTAA GTATGCTAAT CGTGCAAAGG AAATTAAATC TTCTTTGAAG 
AGCAATGTTC TTAATCTCAA CAGTCACATA TCTCAGTATG TAAAGATATG TAATATGCAG 
AAAGCAGAGA TTTTAATGTT GAAAGAAAAA CTAAAGGCCT ATGAAGAACA GAAAGCCCTG 
TCTGACAGAA ATGACTGTGC TAAATTAGTA CATTCAAACC CAGAAGACAG AGAAACTGAA 
AGATTTCAAG AAATTCTGAA CTGCTTGTTC CAGAATCGAG AAGGAATCAG GCAAGAGTAT 
CTGAAGTTAG AAATGTTACT TAAGGCAAAT GCGCTAAAGT CATCCTATCA CCAACAGTGC 
CATAAACAGA TAGAGATGAT GTGTTCTGAA GACAAAGTGG AAAAGGCCAC TTGCAAACGA 
GATCACAGAC TTGAAAAGTT GAAAACCAAC AGCTGTTTTC TAGAGAAAAA GAAAGAAGAG 
GTGTCGAAAC AATTTGATGA AAATACTAAT TGGCTACATC GTGTTGAAAA CGAAATGCGA 
CTCTTGGGTC AAAATGGTGA TATTCCAGAG GCACTCAATA AAGAGCTTCA CTGTCACCAT 
TTACACCTCC AGAACAAAGA ACTGAAAACA CAAATGGCAC ACATGACAGC TCTAGCTTGT 
CTCCAGGAAC AGCAGCACAA GCAAACTGAG GCAGTATTGA ATGCTTTGCT TCCAGTACTA 
AGAAAACAGT ATTGGAAATT GAAAGAAACT GGCCTGTCAA ATGCTGCTTT TGATTCTGAC 
TTCAAAGATA TTGAACATTT AGTAGAGAGA AAAAAGGTGG TGGCTTGGGC TGACCAAACT 
AATGAACATT CAAACCGAAA TGATCTTCCA GGGATTTCTC TTCTTATGAC CTTCCCACAG 
CTTGAACCAA TTCAGTCTAT TTCTTGTTGC ACATCTGTGA GTGATCCTAA CGTGCTTAAA 
CTTACTCCAC AACGAAGAAC CAGAAGAAAA ATAATTCCTT CTCCCTTGAA AGTACAACAT 
ACTCAGAAGT CTGCACTGTC TGAAAGTACA CAGCTCAATG ATTCTTTCAG CAAAGAACTT 
CAGCCTATTG TTTATACGCC AGAGGACTGC AAAAAAGCTC AAGACCTGTT TCCATCCTTA 
ACGAGAACTT CATCGCAGTC TGCAAATGTA ATGAATGACA ATTCCCAGAA AGCACTGTGT 
AGAATAGAGT CTCCTCTCAG CAGAACTGAG TGCAAACAGG GTTTGTACTC TACATCTACT 
CTGTGTGATA GCATCAGAGG CTTGAAGAAT AAATGGCCTG AACAAGAACC ACTAGCAAGC 
AGCAAAAGCA GTGTGCACAG GATAGAGTCT TCTTCATTTT CAACAAAGGA TTCTATGCCT 
GAATCAGCAG GTGTGCCGTC CTACATGGCA ATGACTACTG CTGCAAAGAG GAAATGGAAA 
CAAATGAGTT CTACATCAAA TGCTTCAATC AAATCTGATG AAAGTTGTGG ATTTGCCAAA 
CGCATTCGAC GGGATAATTC AAGCGTTAAG CCTATGCAAG AAAACAGACT GAAAGTGGGC 
TATAAAAGAA ATACCAACAA AACAAATTCA AACATGCTTA GAAAATTTAG AAGAAACACT 
TCAAAAGAAA ATGTACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase.
The Journal of cell biology218(4)1148-1163(2019 Apr)
Fonseca CL,Malaby HLH,Sepaniac LA,Martin W,Byers C,Czechanski A,Messinger D,Tang M,Ohi R,Reinholdt LG,Stumpff J

Kif18a is specifically required for mitotic progression during germ line development.
Developmental biology402(2)253-262(2015 Jun)
Czechanski A,Kim H,Byers C,Greenstein I,Stumpff J,Reinholdt LG

Targeted deletion of Kif18a protects from colitis-associated colorectal (CAC) tumors in mice through impairing Akt phosphorylation.
Biochemical and biophysical research communications438(1)97-102(2013 Aug)
Zhu H,Xu W,Zhang H,Liu J,Xu H,Lu S,Dang S,Kuang Y,Jin X,Wang Z

Germinal Cell Aplasia in Kif18a Mutant Male Mice Due to Impaired Chromosome Congression and Dysregulated BubR1 and CENP-E.
Genes & cancer1(1)26-39(2010 Jan)
Liu XS,Zhao XD,Wang X,Yao YX,Zhang LL,Shu RZ,Ren WH,Huang Y,Huang L,Gu MM,Kuang Y,Wang L,Lu SY,Chi J,Fen JS,Wang YF,Fei J,Dai W,Wang ZG

Analysis of Pax6 contiguous gene deletions in the mouse, Mus musculus, identifies regions distinct from Pax6 responsible for extreme small-eye and belly-spotting phenotypes.
Genetics182(4)1077-88(2009 Aug)
Favor J,Bradley A,Conte N,Janik D,Pretsch W,Reitmeir P,Rosemann M,Schmahl W,Wienberg J,Zaus I

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Kif18a cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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