Eif4h cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4h
Entrez Gene ID	22384
Full Name	eukaryotic translation initiation factor 4H
Synonyms	AU018978,D5Ertd355e,E430026L18Rik,Ef4h,Wbscr1,Wscr1,eIF-4H
General protein information	Preferred Names eukaryotic translation initiation factor 4H Names eukaryotic translation initiation factor 4H williams-Beuren syndrome chromosomal region 1 protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 G2\|5 74.71 cM
Summary	This gene encodes eukaryotic translation initiation factor 4H (eIF4H) that plays a critical role in the process of protein synthesis. The encoded protein is an RNA-binding protein that, in concert with other translation initiation factors, helps unwind the 5' cap-proximal region of mRNA to prepare it for ribosomal attachment. Mice lacking the encoded protein displayed growth retardation with a significant reduction of body weight, a smaller brain volume and altered brain morphology. Behaviorally, mice lacking the encoded protein exhibit severe impairments of fear-related associative learning and memory formation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312867.1	NP_001299796.1	eukaryotic translation initiation factor 4H isoform 2
NM_033561.2	NP_291039.1	eukaryotic translation initiation factor 4H isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EIF4H	XP_001082007.1
Canis lupus familiaris (dog)	EIF4H	XP_005621070.1
Bos taurus (cattle)	EIF4H	NP_001069220.1
Rattus norvegicus (Norway rat)	Eif4h	NP_001006958.1
Pan troglodytes (chimpanzee)	EIF4H	XP_003951096.1
Mus musculus (house mouse)	Eif4h	NP_291039.1
Danio rerio (zebrafish)	eif4h	NP_991258.2
Gallus gallus (chicken)	LOC100859865	XP_003642445.1
Xenopus tropicalis (tropical clawed frog)	eif4h	NP_001096677.1
Homo sapiens (human)	EIF4H	NP_071496.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice with endogenous TDP-43 mutations exhibit gain of splicing function and characteristics of amyotrophic lateral sclerosis.
Fratta P, Sivakumar P, Humphrey J, Lo K, Ricketts T, Oliveira H, Brito-Armas JM, Kalmar B, Ule A, Yu Y, Birsa N, Bodo C, Collins T, Conicella AE, Mejia Maza A, Marrero-Gagliardi A, Stewart M, Mianne J, Corrochano S, Emmett W, Codner G, Groves M, Fukumura R, Gondo Y, Lythgoe M, Pauws E, Peskett E, Stanier P, Teboul L, Hallegger M, Calvo A, Chiò A, Isaacs AM, Fawzi NL, Wang E, Housman DE, Baralle F, Greensmith L, Buratti E, Plagnol V, Fisher EM, Acevedo-Arozena A
The EMBO journal37(11)(2018 06)

The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse.
Collinson JM, Lindström NO, Neves C, Wallace K, Meharg C, Charles RH, Ross ZK, Fraser AM, Mbogo I, Oras K, Nakamoto M, Barker S, Duce S, Miedzybrodzka Z, Vargesson N
Development (Cambridge, England)145(3)(2018 02)

Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB.
Fiume G, Rossi A, de Laurentiis A, Falcone C, Pisano A, Vecchio E, Pontoriero M, Scala I, Scialdone A, Masci FF, Mimmi S, Palmieri C, Scala G, Quinto I
PloS one8(6)e66087(2013)

Growth defects and impaired cognitive-behavioral abilities in mice with knockout for Eif4h, a gene located in the mouse homolog of the Williams-Beuren syndrome critical region.
Capossela S, Muzio L, Bertolo A, Bianchi V, Dati G, Chaabane L, Godi C, Politi LS, Biffo S, D'Adamo P, Mallamaci A, Pannese M
The American journal of pathology180(3)1121-35(2012 Mar)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eif4h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08705	NM_033561.2 Latest version!	Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu69047	NM_001312867.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4h ( NM_033561.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291039.1 Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08705
Clone ID Related Accession (Same CDS sequence)	NM_033561.2
Accession Version	NM_033561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4H isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ158448.1, AC166938.5, AK045004.1 and CF750202.1. On Aug 19, 2015 this sequence version replaced NM_033561.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.215713.1, SRR1660815.100678.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACT TCGATACCTA CGACGATCGG GCCTACAGCA GCTTCGGCGG CGGTAGAGGG 
TCCCGAGGCA GTGCTGGTGG CCATGGGTCC CGGAGCCAGA AGGAGCTACC CACAGAGCCC 
CCCTACACAG CTTACGTAGG AAATCTCCCT TTTAACACAG TTCAGGGTGA CATAGATGCT 
ATCTTTAAGG ATCTCAGCAT ACGGAGTGTG CGGCTAGTCA GAGACAAAGA CACAGACAAA 
TTTAAAGGGT TCTGCTATGT AGAATTTGAT GAGGTGGATT CCCTGAAGGA GGCTCTGACT 
TATGACGGTG CACTCTTGGG TGACCGGTCA CTTCGTGTGG ACATTGCAGA AGGCAGAAAA 
CAAGATAAAG GTGGCTTTGG ATTCAGGAAA GGTGGACCTG ATGACAGAGG AATGGGTGGC 
TCTCGAGAAT CTAGAGGTGG ATGGGATTCC CGGGATGACT TCAACTCTGG CTACAGGGAT 
GACTTCTTAG GGGGTAGGGG GGGCAGTCGC CCTGGGGACC GGCGAGCAGG CCCTCCAATG 
GGGAGTCGCT TTCGAGATGG CCCTCCTCTG CGTGGCTCCA ACATGGACTT CAGAGAACCC 
ACAGAAGAGG AACGAGCACA GAGACCTCGG CTGCAGCTTA AACCTCGGAC AGTGGCAACG 
CCCCTCAATC AAGTAGCCAA CCCCAACTCA GCCATCTTTG GGGGAGCCAG GCCCAGAGAG 
GAAGTGGTTC AGAAGGAGCA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291039.1
CDS	111..857
Translation	MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGMGGSRESRGGWDSRDDFNSGYRDDFLGGRGGSRPGDRRAGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNSAIFGGARPREEVVQKEQE

Target ORF information:

RefSeq Version	NM_033561.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033561.2

ORF Insert Sequence:

ATGGCGGACT TCGATACCTA CGACGATCGG GCCTACAGCA GCTTCGGCGG CGGTAGAGGG 
TCCCGAGGCA GTGCTGGTGG CCATGGGTCC CGGAGCCAGA AGGAGCTACC CACAGAGCCC 
CCCTACACAG CTTACGTAGG AAATCTCCCT TTTAACACAG TTCAGGGTGA CATAGATGCT 
ATCTTTAAGG ATCTCAGCAT ACGGAGTGTG CGGCTAGTCA GAGACAAAGA CACAGACAAA 
TTTAAAGGGT TCTGCTATGT AGAATTTGAT GAGGTGGATT CCCTGAAGGA GGCTCTGACT 
TATGACGGTG CACTCTTGGG TGACCGGTCA CTTCGTGTGG ACATTGCAGA AGGCAGAAAA 
CAAGATAAAG GTGGCTTTGG ATTCAGGAAA GGTGGACCTG ATGACAGAGG AATGGGTGGC 
TCTCGAGAAT CTAGAGGTGG ATGGGATTCC CGGGATGACT TCAACTCTGG CTACAGGGAT 
GACTTCTTAG GGGGTAGGGG GGGCAGTCGC CCTGGGGACC GGCGAGCAGG CCCTCCAATG 
GGGAGTCGCT TTCGAGATGG CCCTCCTCTG CGTGGCTCCA ACATGGACTT CAGAGAACCC 
ACAGAAGAGG AACGAGCACA GAGACCTCGG CTGCAGCTTA AACCTCGGAC AGTGGCAACG 
CCCCTCAATC AAGTAGCCAA CCCCAACTCA GCCATCTTTG GGGGAGCCAG GCCCAGAGAG 
GAAGTGGTTC AGAAGGAGCA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif4h ( NM_001312867.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299796.1 Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69047
Clone ID Related Accession (Same CDS sequence)	NM_001312867.1
Accession Version	NM_001312867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569254400000
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 4H isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ158448.1, AK169407.1, AC166938.5 and CF750202.1. On Aug 19, 2015 this sequence version replaced XM_006504419.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.293533.1, BC014796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACT TCGATACCTA CGACGATCGG GCCTACAGCA GCTTCGGCGG CGGTAGAGGG 
TCCCGAGGCA GTGCTGGTGG CCATGGGTCC CGGAGCCAGA AGGAGCTACC CACAGAGCCC 
CCCTACACAG CTTACGTAGG AAATCTCCCT TTTAACACAG TTCAGGGTGA CATAGATGCT 
ATCTTTAAGG ATCTCAGCAT ACGGAGTGTG CGGCTAGTCA GAGACAAAGA CACAGACAAA 
TTTAAAGGGT TCTGCTATGT AGAATTTGAT GAGGTGGATT CCCTGAAGGA GGCTCTGACT 
TATGACGGTG CACTCTTGGG TGACCGGTCA CTTCGTGTGG ACATTGCAGA AGGCAGAAAA 
CAAGATAAAG GTGGCTTTGG ATTCAGGAAA GGTGGACCTG ATGACAGAGG CTACAGGGAT 
GACTTCTTAG GGGGTAGGGG GGGCAGTCGC CCTGGGGACC GGCGAGCAGG CCCTCCAATG 
GGGAGTCGCT TTCGAGATGG CCCTCCTCTG CGTGGCTCCA ACATGGACTT CAGAGAACCC 
ACAGAAGAGG AACGAGCACA GAGACCTCGG CTGCAGCTTA AACCTCGGAC AGTGGCAACG 
CCCCTCAATC AAGTAGCCAA CCCCAACTCA GCCATCTTTG GGGGAGCCAG GCCCAGAGAG 
GAAGTGGTTC AGAAGGAGCA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299796.1
CDS	111..797
Translation	MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGYRDDFLGGRGGSRPGDRRAGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNSAIFGGARPREEVVQKEQE

Target ORF information:

RefSeq Version	NM_001312867.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4H (Eif4h), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312867.1

ORF Insert Sequence:

ATGGCGGACT TCGATACCTA CGACGATCGG GCCTACAGCA GCTTCGGCGG CGGTAGAGGG 
TCCCGAGGCA GTGCTGGTGG CCATGGGTCC CGGAGCCAGA AGGAGCTACC CACAGAGCCC 
CCCTACACAG CTTACGTAGG AAATCTCCCT TTTAACACAG TTCAGGGTGA CATAGATGCT 
ATCTTTAAGG ATCTCAGCAT ACGGAGTGTG CGGCTAGTCA GAGACAAAGA CACAGACAAA 
TTTAAAGGGT TCTGCTATGT AGAATTTGAT GAGGTGGATT CCCTGAAGGA GGCTCTGACT 
TATGACGGTG CACTCTTGGG TGACCGGTCA CTTCGTGTGG ACATTGCAGA AGGCAGAAAA 
CAAGATAAAG GTGGCTTTGG ATTCAGGAAA GGTGGACCTG ATGACAGAGG CTACAGGGAT 
GACTTCTTAG GGGGTAGGGG GGGCAGTCGC CCTGGGGACC GGCGAGCAGG CCCTCCAATG 
GGGAGTCGCT TTCGAGATGG CCCTCCTCTG CGTGGCTCCA ACATGGACTT CAGAGAACCC 
ACAGAAGAGG AACGAGCACA GAGACCTCGG CTGCAGCTTA AACCTCGGAC AGTGGCAACG 
CCCCTCAATC AAGTAGCCAA CCCCAACTCA GCCATCTTTG GGGGAGCCAG GCCCAGAGAG 
GAAGTGGTTC AGAAGGAGCA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice with endogenous TDP-43 mutations exhibit gain of splicing function and characteristics of amyotrophic lateral sclerosis.
The EMBO journal37(11)(2018 06)
Fratta P,Sivakumar P,Humphrey J,Lo K,Ricketts T,Oliveira H,Brito-Armas JM,Kalmar B,Ule A,Yu Y,Birsa N,Bodo C,Collins T,Conicella AE,Mejia Maza A,Marrero-Gagliardi A,Stewart M,Mianne J,Corrochano S,Emmett W,Codner G,Groves M,Fukumura R,Gondo Y,Lythgoe M,Pauws E,Peskett E,Stanier P,Teboul L,Hallegger M,Calvo A,Chiò A,Isaacs AM,Fawzi NL,Wang E,Housman DE,Baralle F,Greensmith L,Buratti E,Plagnol V,Fisher EM,Acevedo-Arozena A

The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse.
Development (Cambridge, England)145(3)(2018 02)
Collinson JM,Lindström NO,Neves C,Wallace K,Meharg C,Charles RH,Ross ZK,Fraser AM,Mbogo I,Oras K,Nakamoto M,Barker S,Duce S,Miedzybrodzka Z,Vargesson N

Eukaryotic Initiation Factor 4H Is under Transcriptional Control of p65/NF-κB.
PloS one8(6)e66087(2013)
Fiume G,Rossi A,de Laurentiis A,Falcone C,Pisano A,Vecchio E,Pontoriero M,Scala I,Scialdone A,Masci FF,Mimmi S,Palmieri C,Scala G,Quinto I

Growth defects and impaired cognitive-behavioral abilities in mice with knockout for Eif4h, a gene located in the mouse homolog of the Williams-Beuren syndrome critical region.
The American journal of pathology180(3)1121-35(2012 Mar)
Capossela S,Muzio L,Bertolo A,Bianchi V,Dati G,Chaabane L,Godi C,Politi LS,Biffo S,D'Adamo P,Mallamaci A,Pannese M

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

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Eif4h cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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