Washc5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Washc5
Entrez Gene ID	223593
Full Name	WASH complex subunit 5
Synonyms	AL022848,C76463,E430025E21Rik,Kiaa0196,mKIAA0196,strumpellin
General protein information	Preferred Names WASH complex subunit 5 Names WASH complex subunit 5 WASH complex subunit strumpellin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_153548.2	NP_705776.2	WASH complex subunit 5
NM_153548.3	NP_705776.2	WASH complex subunit 5
XM_006520759.3	XP_006520822.1	WASH complex subunit strumpellin isoform X1
XM_006520759.4	XP_006520822.1	WASH complex subunit 5 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KIAA0196	XP_418441.2
Mus musculus (house mouse)	E430025E21Rik	NP_705776.2
Rattus norvegicus (Norway rat)	RGD1564420	XP_002729862.2
Xenopus tropicalis (tropical clawed frog)	kiaa0196	NP_001004970.1
Oryza sativa (rice)	Os04g0398800	NP_001052675.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007019	XP_308754.3
Caenorhabditis elegans (roundworm)	T05E7.3	NP_491784.1
Bos taurus (cattle)	KIAA0196	XP_002692668.1
Homo sapiens (human)	KIAA0196	NP_055661.3
Pan troglodytes (chimpanzee)	KIAA0196	XP_519952.2
Macaca mulatta (Rhesus monkey)	LOC706857	XP_002805529.1
Canis lupus familiaris (dog)	KIAA0196	XP_532327.2
Danio rerio (zebrafish)	strumpellin	NP_956477.1
Drosophila melanogaster (fruit fly)	CG12272	NP_001163454.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of N471D strumpellin leads to defects in the endolysosomal system.
Song L, Rijal R, Karow M, Stumpf M, Hahn O, Park L, Insall R, Schröder R, Hofmann A, Clemen CS, Eichinger L
Disease models & mechanisms11(9)(2018 09)

Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.
Tyrrell BJ, Woodham EF, Spence HJ, Strathdee D, Insall RH, Machesky LM
Pigment cell & melanoma research29(5)559-71(2016 09)

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
Bartuzi P, Billadeau DD, Favier R, Rong S, Dekker D, Fedoseienko A, Fieten H, Wijers M, Levels JH, Huijkman N, Kloosterhuis N, van der Molen H, Brufau G, Groen AK, Elliott AM, Kuivenhoven JA, Plecko B, Grangl G, McGaughran J, Horton JD, Burstein E, Hofker MH, van de Sluis B
Nature communications710961(2016 Mar)

The spectrum of KIAA0196 variants, and characterization of a murine knockout: implications for the mutational mechanism in hereditary spastic paraplegia type SPG8.
Jahic A, Khundadze M, Jaenisch N, Schüle R, Klimpe S, Klebe S, Frahm C, Kassubek J, Stevanin G, Schöls L, Brice A, Hübner CA, Beetz C
Orphanet journal of rare diseases10147(2015 Nov)

WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes.
Wang F, Zhang L, Zhang GL, Wang ZB, Cui XS, Kim NH, Sun SC
Scientific reports45596(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Washc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Washc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02838	NM_153548.3 Latest version!	Mus musculus WASH complex subunit 5 (Washc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu51164	XM_006520759.4 Latest version!	Mus musculus WASH complex subunit 5 (Washc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu02838	NM_153548.2	Mus musculus WASH complex subunit 5 (Washc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu51164	XM_006520759.3	Mus musculus RIKEN cDNA E430025E21 gene (E430025E21Rik), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Washc5 ( NM_153548.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705776.2 Mus musculus WASH complex subunit 5 (Washc5), mRNA.

Price & Availability↑

CloneID	OMu02838
Clone ID Related Accession (Same CDS sequence)	NM_153548.2 , NM_153548.3
Accession Version	NM_153548.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1493913600000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067035.1. On Apr 2, 2004 this sequence version replaced NM_153548.1. ##Evidence-Data-START## Transcript exon combination :: BC067035.1, AK128970.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTTGGACT TCCTGGCTGA GAATAATCTC TGTGGCCAGG CCATCCTGAG GATCGTCTCC 
TGTGGCAATG CCATCATTGC CGAGGTGCTG CGGCTCTCTG AGTTCATTCC TGCTGTGTTC 
CTGCTGAAAG ACCGGGCTGA CCAGCAGAGA TATGGAGACA TCATCTTTGA CTTCAGCTAC 
TTTAAGGGTC CAGAGTTCTG GGAAAGCAAA CTGGAAGCCA AGCCTGAGCT GCAGGATTTA 
GATGAAGAAT TTCGGGAAAA CAACATAGAA ATCGTGACCA GATTTTATTT AGCCTTTCAA 
AGTGTGCATA AATACATTGT AGACTTAAAC AGATACCTCG ATGACCTCAA TGAAGGAGTT 
TATATTCAGC AAACCTTAGA AACCGTGCTT CTCAGTGAAG ATGGAAAACA ACTTCTATGT 
GAAGCCCTGT ACTTATATGG AGTTATGCTG CTTGTCATCG ACCAGAAGAT TGAAGGAGAA 
GTCAGAGAGA GAATGCTGGT TTCTTACTAT CGATACAGTG CTGCTCGGTC CTCTGCTGAC 
TCAAACATGG ATGATATCTG TAAGCTGCTA CGAAGCACAG GATATTCCAG CCAGCCAGGA 
GCCAAAAGAC CACCCAACTA TCCTGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAGACG 
TTCATCAGCA TGGTCATCGG ACGCCTGAGG TCTGATGACA TTTACAACCA GGTCTCTGCC 
TACCCGCTGC CGGAGCACCG CAGCACTGCC CTGGCCAATC AAGCCGCCAT GCTCTACGTG 
ATTCTCTATT TTGAACCTTC CATTCTTCAC ACCCACCAAG CAAAAATGAG AGAGATCGTG 
GATAAGTACT TTCCAGATAA CTGGGTAATC AGCATCTATA TGGGTATCAC TGTTAATCTA 
GCTGATGCCT GGGAACCTTA CAAAGCTGCG AAAACTGCTC TCAACAACAC CCTTGACCTC 
GCCAATGTCA AAGAACAGGC AAGCAGATAC GCTAGTGTCA GCGACAGAGT GCGCGCTCAG 
GTGCAGCAGT TTCTGAAAGA GGGCTACCTG AGGGAGGAGG TGCTTCTGGA CAACATCCCC 
AGGCTGCTGA ACTGCCTGAG AGACTGCAAT GTCGCCATCC GCTGGCTAAT GCTCCACACA 
GCCGACTCAG CCTGTGACCC AAACAACAAG CGCCTTCGTC AAATCAAGGA CCAGATTCTG 
GCAGACTCTA GGTACAATCC CAAGATCCTC TTCCAGTTGC TATTGGACAC TGCACAATTC 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCCG AGAAACAGTC CAAGTGGGAG 
CATTACAAGA AGGAGGGCTC TGAGCGCATG ACCGAGCTTG CTGACGTCTT TTCAGGGGTG 
AAACCACTAA CCCGAGTGGA GAAAAACGAA AATCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAC TGTCTCTAAA TTATGATGAT TCTACTGCTG CAGGCAGAAA AACTGTGCAA 
CTAATACAAG CTCTGGAAGA GGTTCAGGAG TTTCACCAGT TGGAATCAAA TCTACAAGTT 
TGCCAGTTTC TAGCTGACAC CCGAAAGTTT CTCCATCAAA TGATCAGAAC CATCAACATC 
AAGGAGGAAG TCCTGATCAC AGTGCAGATC ATCGGGGACC TTTCCTTTGC CTGGCAGTTA 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAACCCATC CATGGTTACC 
AAACTCAGAG CCACGTTCCT AAAGCTTGCC TCTGCCCTTG ACCTTCCCCT GCTACGTATT 
AATCAAGCAA ATAGCCCTGA TCTCCTCAGT GTGTCTCAGT ATTATTCAGG AGAATTGGTG 
TCATACGTGA GAAAGGTTTT GCAGATCATT CCAGAAAGCA TGTTCACATC TCTCCTGAAG 
ATCATAAAGC TTCAGACCCA TGACATCATG GAAGTGCCTA CACGCCTGGA CAAGGACAAG 
CTGCGGGACT ACGCTCAGCT TGGCCCGAGA TATGAGGTTG CCAAGCTCAC TCACGCCATC 
TCCATCTTTA CTGAGGGCAT CTTGATGATG AAAACGACCC TGGTGGGCAT CATCAAGGTG 
GATCCCAAGC AGTTGCTGGA AGACGGAATA AGGAAAGAGC TGGTTAAACG CGTGGCTTTC 
GCCCTTCACC GGGGACTGAT ATTCAACCCT CGTGCCAAGC CAAGTGAGTT GATGCCCAAA 
CTGAAAGAGC TGGGGGCCAC CATGGATGGG TTCCATCGCT CCTTTGAGTA TATACAGGAC 
TACGTCAGCA TTTACGGTCT GAAGATTTGG CAGGAGGAAG TCTCTCGTAT TATAAATTAT 
AACGTGGAAC AGGAGTGTAA TAACTTCTTA AGAACAAAGA TTCAAGACTG GCAGAGTATG 
TACCAATCCA CTCATATACC AATCCCGAAG TTTGCACCTG TGGATGAGTC TATAACATTT 
ATTGGTCGAC TCTGCAGAGA AATCTTGCGG ATCACAGACC CAAAAATGAC ATGTTACATA 
GACCAGCTGA ACACTTGGTA TGACGTGAAA ACTCATCAAG AAGTGACCAG CAGTCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGGACT TTTGGTCTGA ATGGATTGGA CAGGCTTCTG 
TGCTTCATGA TTGTGAAAGA GCTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AAAGAGAGAA CTGTTCAAGA AACTTTAAAG ATGCTCATGA GTGCTGTTAA CCCTCTAAAG 
AGCATAGTGG CAAATTCAAG TAAAGTTTAT CTTTCTGCCA TCACCAAAAC ACAGAAGATC 
TGGAGTGCTT ACCTGGAGGC TATAATGAAG GTTGGACAGA TGCAGATCCT GAGACAACAG 
ATCGCCAATG AGCTAAACTC TTCCTGTCGA TTTGATTCCA GACATCTGGC AGCTGCCCTG 
GATAATCTCA ATAAGGCTCT CCTGGCAGAC ATCGAAGCCC ACTACCGGGA CCCTTCGCTT 
CCTTACCCCA AAGAAGATAA CACACTCTTA TATGAAATCA CAGCCTATCT GGAGGCGGCT 
GGCATTCACA ACCCCCTGAA TAAGATTTAC ATAACAACGA AGCGCTTACC CTATTTCCCG 
ATTGTCAACT TTCTGTTTTT GATCGCGCAG TTGCCAAAAC TTCAGTATAA CAAAAATCTA 
GGCATGGTCT GCCGGAAGCC TGCCGACCCT GTTGACTGGC CACCGCTCGT CCTAGGTCTG 
CTCACGCTGC TGAAGCAGTT CCACTCCCGG TACACCGAGC AATTCCTGGC ACTGATTGGC 
CAGTTTATTC GCTCCACAAT GGAGCAGTGC ACAAGCCAGA AGATGCCTGA AATGCCTGCA 
GATGCTGTGG GCGCCCTTCT CTTCCTGGAG GATTATGTTC GGTATACAAA ACTCCCCCGA 
AGGGTGGCTG AAGCCCATGT GCCTAACTTC ATTTTTGATG AATTCCGAAC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705776.2
CDS	233..3712
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAEVLRLSEFIPAVFLLKDRADQQRYGDIIFDFSYFKGPEFWESKLEAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLSEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPPNYPESYFQRVPINETFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLANVKEQASRYASVSDRVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMSAVNPLKSIVANSSKVYLSAITKTQKIWSAYLEAIMKVGQMQILRQQIANELNSSCRFDSRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	NM_153548.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 5 (Washc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153548.2

ORF Insert Sequence:

ATGTTGGACT TCCTGGCTGA GAATAATCTC TGTGGCCAGG CCATCCTGAG GATCGTCTCC 
TGTGGCAATG CCATCATTGC CGAGGTGCTG CGGCTCTCTG AGTTCATTCC TGCTGTGTTC 
CTGCTGAAAG ACCGGGCTGA CCAGCAGAGA TATGGAGACA TCATCTTTGA CTTCAGCTAC 
TTTAAGGGTC CAGAGTTCTG GGAAAGCAAA CTGGAAGCCA AGCCTGAGCT GCAGGATTTA 
GATGAAGAAT TTCGGGAAAA CAACATAGAA ATCGTGACCA GATTTTATTT AGCCTTTCAA 
AGTGTGCATA AATACATTGT AGACTTAAAC AGATACCTCG ATGACCTCAA TGAAGGAGTT 
TATATTCAGC AAACCTTAGA AACCGTGCTT CTCAGTGAAG ATGGAAAACA ACTTCTATGT 
GAAGCCCTGT ACTTATATGG AGTTATGCTG CTTGTCATCG ACCAGAAGAT TGAAGGAGAA 
GTCAGAGAGA GAATGCTGGT TTCTTACTAT CGATACAGTG CTGCTCGGTC CTCTGCTGAC 
TCAAACATGG ATGATATCTG TAAGCTGCTA CGAAGCACAG GATATTCCAG CCAGCCAGGA 
GCCAAAAGAC CACCCAACTA TCCTGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAGACG 
TTCATCAGCA TGGTCATCGG ACGCCTGAGG TCTGATGACA TTTACAACCA GGTCTCTGCC 
TACCCGCTGC CGGAGCACCG CAGCACTGCC CTGGCCAATC AAGCCGCCAT GCTCTACGTG 
ATTCTCTATT TTGAACCTTC CATTCTTCAC ACCCACCAAG CAAAAATGAG AGAGATCGTG 
GATAAGTACT TTCCAGATAA CTGGGTAATC AGCATCTATA TGGGTATCAC TGTTAATCTA 
GCTGATGCCT GGGAACCTTA CAAAGCTGCG AAAACTGCTC TCAACAACAC CCTTGACCTC 
GCCAATGTCA AAGAACAGGC AAGCAGATAC GCTAGTGTCA GCGACAGAGT GCGCGCTCAG 
GTGCAGCAGT TTCTGAAAGA GGGCTACCTG AGGGAGGAGG TGCTTCTGGA CAACATCCCC 
AGGCTGCTGA ACTGCCTGAG AGACTGCAAT GTCGCCATCC GCTGGCTAAT GCTCCACACA 
GCCGACTCAG CCTGTGACCC AAACAACAAG CGCCTTCGTC AAATCAAGGA CCAGATTCTG 
GCAGACTCTA GGTACAATCC CAAGATCCTC TTCCAGTTGC TATTGGACAC TGCACAATTC 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCCG AGAAACAGTC CAAGTGGGAG 
CATTACAAGA AGGAGGGCTC TGAGCGCATG ACCGAGCTTG CTGACGTCTT TTCAGGGGTG 
AAACCACTAA CCCGAGTGGA GAAAAACGAA AATCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAC TGTCTCTAAA TTATGATGAT TCTACTGCTG CAGGCAGAAA AACTGTGCAA 
CTAATACAAG CTCTGGAAGA GGTTCAGGAG TTTCACCAGT TGGAATCAAA TCTACAAGTT 
TGCCAGTTTC TAGCTGACAC CCGAAAGTTT CTCCATCAAA TGATCAGAAC CATCAACATC 
AAGGAGGAAG TCCTGATCAC AGTGCAGATC ATCGGGGACC TTTCCTTTGC CTGGCAGTTA 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAACCCATC CATGGTTACC 
AAACTCAGAG CCACGTTCCT AAAGCTTGCC TCTGCCCTTG ACCTTCCCCT GCTACGTATT 
AATCAAGCAA ATAGCCCTGA TCTCCTCAGT GTGTCTCAGT ATTATTCAGG AGAATTGGTG 
TCATACGTGA GAAAGGTTTT GCAGATCATT CCAGAAAGCA TGTTCACATC TCTCCTGAAG 
ATCATAAAGC TTCAGACCCA TGACATCATG GAAGTGCCTA CACGCCTGGA CAAGGACAAG 
CTGCGGGACT ACGCTCAGCT TGGCCCGAGA TATGAGGTTG CCAAGCTCAC TCACGCCATC 
TCCATCTTTA CTGAGGGCAT CTTGATGATG AAAACGACCC TGGTGGGCAT CATCAAGGTG 
GATCCCAAGC AGTTGCTGGA AGACGGAATA AGGAAAGAGC TGGTTAAACG CGTGGCTTTC 
GCCCTTCACC GGGGACTGAT ATTCAACCCT CGTGCCAAGC CAAGTGAGTT GATGCCCAAA 
CTGAAAGAGC TGGGGGCCAC CATGGATGGG TTCCATCGCT CCTTTGAGTA TATACAGGAC 
TACGTCAGCA TTTACGGTCT GAAGATTTGG CAGGAGGAAG TCTCTCGTAT TATAAATTAT 
AACGTGGAAC AGGAGTGTAA TAACTTCTTA AGAACAAAGA TTCAAGACTG GCAGAGTATG 
TACCAATCCA CTCATATACC AATCCCGAAG TTTGCACCTG TGGATGAGTC TATAACATTT 
ATTGGTCGAC TCTGCAGAGA AATCTTGCGG ATCACAGACC CAAAAATGAC ATGTTACATA 
GACCAGCTGA ACACTTGGTA TGACGTGAAA ACTCATCAAG AAGTGACCAG CAGTCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGGACT TTTGGTCTGA ATGGATTGGA CAGGCTTCTG 
TGCTTCATGA TTGTGAAAGA GCTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AAAGAGAGAA CTGTTCAAGA AACTTTAAAG ATGCTCATGA GTGCTGTTAA CCCTCTAAAG 
AGCATAGTGG CAAATTCAAG TAAAGTTTAT CTTTCTGCCA TCACCAAAAC ACAGAAGATC 
TGGAGTGCTT ACCTGGAGGC TATAATGAAG GTTGGACAGA TGCAGATCCT GAGACAACAG 
ATCGCCAATG AGCTAAACTC TTCCTGTCGA TTTGATTCCA GACATCTGGC AGCTGCCCTG 
GATAATCTCA ATAAGGCTCT CCTGGCAGAC ATCGAAGCCC ACTACCGGGA CCCTTCGCTT 
CCTTACCCCA AAGAAGATAA CACACTCTTA TATGAAATCA CAGCCTATCT GGAGGCGGCT 
GGCATTCACA ACCCCCTGAA TAAGATTTAC ATAACAACGA AGCGCTTACC CTATTTCCCG 
ATTGTCAACT TTCTGTTTTT GATCGCGCAG TTGCCAAAAC TTCAGTATAA CAAAAATCTA 
GGCATGGTCT GCCGGAAGCC TGCCGACCCT GTTGACTGGC CACCGCTCGT CCTAGGTCTG 
CTCACGCTGC TGAAGCAGTT CCACTCCCGG TACACCGAGC AATTCCTGGC ACTGATTGGC 
CAGTTTATTC GCTCCACAAT GGAGCAGTGC ACAAGCCAGA AGATGCCTGA AATGCCTGCA 
GATGCTGTGG GCGCCCTTCT CTTCCTGGAG GATTATGTTC GGTATACAAA ACTCCCCCGA 
AGGGTGGCTG AAGCCCATGT GCCTAACTTC ATTTTTGATG AATTCCGAAC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc5 ( NM_153548.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705776.2 Mus musculus WASH complex subunit 5 (Washc5), mRNA.

Price & Availability↑

CloneID	OMu02838
Clone ID Related Accession (Same CDS sequence)	NM_153548.2 , NM_153548.3
Accession Version	NM_153548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553270400000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113269.13 and BC067035.1. On Jul 12, 2018 this sequence version replaced NM_153548.2. ##Evidence-Data-START## Transcript exon combination :: BC067035.1, AK128970.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGACT TCCTGGCTGA GAATAATCTC TGTGGCCAGG CCATCCTGAG GATCGTCTCC 
TGTGGCAATG CCATCATTGC CGAGGTGCTG CGGCTCTCTG AGTTCATTCC TGCTGTGTTC 
CTGCTGAAAG ACCGGGCTGA CCAGCAGAGA TATGGAGACA TCATCTTTGA CTTCAGCTAC 
TTTAAGGGTC CAGAGTTCTG GGAAAGCAAA CTGGAAGCCA AGCCTGAGCT GCAGGATTTA 
GATGAAGAAT TTCGGGAAAA CAACATAGAA ATCGTGACCA GATTTTATTT AGCCTTTCAA 
AGTGTGCATA AATACATTGT AGACTTAAAC AGATACCTCG ATGACCTCAA TGAAGGAGTT 
TATATTCAGC AAACCTTAGA AACCGTGCTT CTCAGTGAAG ATGGAAAACA ACTTCTATGT 
GAAGCCCTGT ACTTATATGG AGTTATGCTG CTTGTCATCG ACCAGAAGAT TGAAGGAGAA 
GTCAGAGAGA GAATGCTGGT TTCTTACTAT CGATACAGTG CTGCTCGGTC CTCTGCTGAC 
TCAAACATGG ATGATATCTG TAAGCTGCTA CGAAGCACAG GATATTCCAG CCAGCCAGGA 
GCCAAAAGAC CACCCAACTA TCCTGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAGACG 
TTCATCAGCA TGGTCATCGG ACGCCTGAGG TCTGATGACA TTTACAACCA GGTCTCTGCC 
TACCCGCTGC CGGAGCACCG CAGCACTGCC CTGGCCAATC AAGCCGCCAT GCTCTACGTG 
ATTCTCTATT TTGAACCTTC CATTCTTCAC ACCCACCAAG CAAAAATGAG AGAGATCGTG 
GATAAGTACT TTCCAGATAA CTGGGTAATC AGCATCTATA TGGGTATCAC TGTTAATCTA 
GCTGATGCCT GGGAACCTTA CAAAGCTGCG AAAACTGCTC TCAACAACAC CCTTGACCTC 
GCCAATGTCA AAGAACAGGC AAGCAGATAC GCTAGTGTCA GCGACAGAGT GCGCGCTCAG 
GTGCAGCAGT TTCTGAAAGA GGGCTACCTG AGGGAGGAGG TGCTTCTGGA CAACATCCCC 
AGGCTGCTGA ACTGCCTGAG AGACTGCAAT GTCGCCATCC GCTGGCTAAT GCTCCACACA 
GCCGACTCAG CCTGTGACCC AAACAACAAG CGCCTTCGTC AAATCAAGGA CCAGATTCTG 
GCAGACTCTA GGTACAATCC CAAGATCCTC TTCCAGTTGC TATTGGACAC TGCACAATTC 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCCG AGAAACAGTC CAAGTGGGAG 
CATTACAAGA AGGAGGGCTC TGAGCGCATG ACCGAGCTTG CTGACGTCTT TTCAGGGGTG 
AAACCACTAA CCCGAGTGGA GAAAAACGAA AATCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAC TGTCTCTAAA TTATGATGAT TCTACTGCTG CAGGCAGAAA AACTGTGCAA 
CTAATACAAG CTCTGGAAGA GGTTCAGGAG TTTCACCAGT TGGAATCAAA TCTACAAGTT 
TGCCAGTTTC TAGCTGACAC CCGAAAGTTT CTCCATCAAA TGATCAGAAC CATCAACATC 
AAGGAGGAAG TCCTGATCAC AGTGCAGATC ATCGGGGACC TTTCCTTTGC CTGGCAGTTA 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAACCCATC CATGGTTACC 
AAACTCAGAG CCACGTTCCT AAAGCTTGCC TCTGCCCTTG ACCTTCCCCT GCTACGTATT 
AATCAAGCAA ATAGCCCTGA TCTCCTCAGT GTGTCTCAGT ATTATTCAGG AGAATTGGTG 
TCATACGTGA GAAAGGTTTT GCAGATCATT CCAGAAAGCA TGTTCACATC TCTCCTGAAG 
ATCATAAAGC TTCAGACCCA TGACATCATG GAAGTGCCTA CACGCCTGGA CAAGGACAAG 
CTGCGGGACT ACGCTCAGCT TGGCCCGAGA TATGAGGTTG CCAAGCTCAC TCACGCCATC 
TCCATCTTTA CTGAGGGCAT CTTGATGATG AAAACGACCC TGGTGGGCAT CATCAAGGTG 
GATCCCAAGC AGTTGCTGGA AGACGGAATA AGGAAAGAGC TGGTTAAACG CGTGGCTTTC 
GCCCTTCACC GGGGACTGAT ATTCAACCCT CGTGCCAAGC CAAGTGAGTT GATGCCCAAA 
CTGAAAGAGC TGGGGGCCAC CATGGATGGG TTCCATCGCT CCTTTGAGTA TATACAGGAC 
TACGTCAGCA TTTACGGTCT GAAGATTTGG CAGGAGGAAG TCTCTCGTAT TATAAATTAT 
AACGTGGAAC AGGAGTGTAA TAACTTCTTA AGAACAAAGA TTCAAGACTG GCAGAGTATG 
TACCAATCCA CTCATATACC AATCCCGAAG TTTGCACCTG TGGATGAGTC TATAACATTT 
ATTGGTCGAC TCTGCAGAGA AATCTTGCGG ATCACAGACC CAAAAATGAC ATGTTACATA 
GACCAGCTGA ACACTTGGTA TGACGTGAAA ACTCATCAAG AAGTGACCAG CAGTCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGGACT TTTGGTCTGA ATGGATTGGA CAGGCTTCTG 
TGCTTCATGA TTGTGAAAGA GCTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AAAGAGAGAA CTGTTCAAGA AACTTTAAAG ATGCTCATGA GTGCTGTTAA CCCTCTAAAG 
AGCATAGTGG CAAATTCAAG TAAAGTTTAT CTTTCTGCCA TCACCAAAAC ACAGAAGATC 
TGGAGTGCTT ACCTGGAGGC TATAATGAAG GTTGGACAGA TGCAGATCCT GAGACAACAG 
ATCGCCAATG AGCTAAACTC TTCCTGTCGA TTTGATTCCA GACATCTGGC AGCTGCCCTG 
GATAATCTCA ATAAGGCTCT CCTGGCAGAC ATCGAAGCCC ACTACCGGGA CCCTTCGCTT 
CCTTACCCCA AAGAAGATAA CACACTCTTA TATGAAATCA CAGCCTATCT GGAGGCGGCT 
GGCATTCACA ACCCCCTGAA TAAGATTTAC ATAACAACGA AGCGCTTACC CTATTTCCCG 
ATTGTCAACT TTCTGTTTTT GATCGCGCAG TTGCCAAAAC TTCAGTATAA CAAAAATCTA 
GGCATGGTCT GCCGGAAGCC TGCCGACCCT GTTGACTGGC CACCGCTCGT CCTAGGTCTG 
CTCACGCTGC TGAAGCAGTT CCACTCCCGG TACACCGAGC AATTCCTGGC ACTGATTGGC 
CAGTTTATTC GCTCCACAAT GGAGCAGTGC ACAAGCCAGA AGATGCCTGA AATGCCTGCA 
GATGCTGTGG GCGCCCTTCT CTTCCTGGAG GATTATGTTC GGTATACAAA ACTCCCCCGA 
AGGGTGGCTG AAGCCCATGT GCCTAACTTC ATTTTTGATG AATTCCGAAC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705776.2
CDS	268..3747
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAEVLRLSEFIPAVFLLKDRADQQRYGDIIFDFSYFKGPEFWESKLEAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLSEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPPNYPESYFQRVPINETFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLANVKEQASRYASVSDRVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMSAVNPLKSIVANSSKVYLSAITKTQKIWSAYLEAIMKVGQMQILRQQIANELNSSCRFDSRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	NM_153548.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 5 (Washc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153548.3

ORF Insert Sequence:

ATGTTGGACT TCCTGGCTGA GAATAATCTC TGTGGCCAGG CCATCCTGAG GATCGTCTCC 
TGTGGCAATG CCATCATTGC CGAGGTGCTG CGGCTCTCTG AGTTCATTCC TGCTGTGTTC 
CTGCTGAAAG ACCGGGCTGA CCAGCAGAGA TATGGAGACA TCATCTTTGA CTTCAGCTAC 
TTTAAGGGTC CAGAGTTCTG GGAAAGCAAA CTGGAAGCCA AGCCTGAGCT GCAGGATTTA 
GATGAAGAAT TTCGGGAAAA CAACATAGAA ATCGTGACCA GATTTTATTT AGCCTTTCAA 
AGTGTGCATA AATACATTGT AGACTTAAAC AGATACCTCG ATGACCTCAA TGAAGGAGTT 
TATATTCAGC AAACCTTAGA AACCGTGCTT CTCAGTGAAG ATGGAAAACA ACTTCTATGT 
GAAGCCCTGT ACTTATATGG AGTTATGCTG CTTGTCATCG ACCAGAAGAT TGAAGGAGAA 
GTCAGAGAGA GAATGCTGGT TTCTTACTAT CGATACAGTG CTGCTCGGTC CTCTGCTGAC 
TCAAACATGG ATGATATCTG TAAGCTGCTA CGAAGCACAG GATATTCCAG CCAGCCAGGA 
GCCAAAAGAC CACCCAACTA TCCTGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAGACG 
TTCATCAGCA TGGTCATCGG ACGCCTGAGG TCTGATGACA TTTACAACCA GGTCTCTGCC 
TACCCGCTGC CGGAGCACCG CAGCACTGCC CTGGCCAATC AAGCCGCCAT GCTCTACGTG 
ATTCTCTATT TTGAACCTTC CATTCTTCAC ACCCACCAAG CAAAAATGAG AGAGATCGTG 
GATAAGTACT TTCCAGATAA CTGGGTAATC AGCATCTATA TGGGTATCAC TGTTAATCTA 
GCTGATGCCT GGGAACCTTA CAAAGCTGCG AAAACTGCTC TCAACAACAC CCTTGACCTC 
GCCAATGTCA AAGAACAGGC AAGCAGATAC GCTAGTGTCA GCGACAGAGT GCGCGCTCAG 
GTGCAGCAGT TTCTGAAAGA GGGCTACCTG AGGGAGGAGG TGCTTCTGGA CAACATCCCC 
AGGCTGCTGA ACTGCCTGAG AGACTGCAAT GTCGCCATCC GCTGGCTAAT GCTCCACACA 
GCCGACTCAG CCTGTGACCC AAACAACAAG CGCCTTCGTC AAATCAAGGA CCAGATTCTG 
GCAGACTCTA GGTACAATCC CAAGATCCTC TTCCAGTTGC TATTGGACAC TGCACAATTC 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCCG AGAAACAGTC CAAGTGGGAG 
CATTACAAGA AGGAGGGCTC TGAGCGCATG ACCGAGCTTG CTGACGTCTT TTCAGGGGTG 
AAACCACTAA CCCGAGTGGA GAAAAACGAA AATCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAC TGTCTCTAAA TTATGATGAT TCTACTGCTG CAGGCAGAAA AACTGTGCAA 
CTAATACAAG CTCTGGAAGA GGTTCAGGAG TTTCACCAGT TGGAATCAAA TCTACAAGTT 
TGCCAGTTTC TAGCTGACAC CCGAAAGTTT CTCCATCAAA TGATCAGAAC CATCAACATC 
AAGGAGGAAG TCCTGATCAC AGTGCAGATC ATCGGGGACC TTTCCTTTGC CTGGCAGTTA 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAACCCATC CATGGTTACC 
AAACTCAGAG CCACGTTCCT AAAGCTTGCC TCTGCCCTTG ACCTTCCCCT GCTACGTATT 
AATCAAGCAA ATAGCCCTGA TCTCCTCAGT GTGTCTCAGT ATTATTCAGG AGAATTGGTG 
TCATACGTGA GAAAGGTTTT GCAGATCATT CCAGAAAGCA TGTTCACATC TCTCCTGAAG 
ATCATAAAGC TTCAGACCCA TGACATCATG GAAGTGCCTA CACGCCTGGA CAAGGACAAG 
CTGCGGGACT ACGCTCAGCT TGGCCCGAGA TATGAGGTTG CCAAGCTCAC TCACGCCATC 
TCCATCTTTA CTGAGGGCAT CTTGATGATG AAAACGACCC TGGTGGGCAT CATCAAGGTG 
GATCCCAAGC AGTTGCTGGA AGACGGAATA AGGAAAGAGC TGGTTAAACG CGTGGCTTTC 
GCCCTTCACC GGGGACTGAT ATTCAACCCT CGTGCCAAGC CAAGTGAGTT GATGCCCAAA 
CTGAAAGAGC TGGGGGCCAC CATGGATGGG TTCCATCGCT CCTTTGAGTA TATACAGGAC 
TACGTCAGCA TTTACGGTCT GAAGATTTGG CAGGAGGAAG TCTCTCGTAT TATAAATTAT 
AACGTGGAAC AGGAGTGTAA TAACTTCTTA AGAACAAAGA TTCAAGACTG GCAGAGTATG 
TACCAATCCA CTCATATACC AATCCCGAAG TTTGCACCTG TGGATGAGTC TATAACATTT 
ATTGGTCGAC TCTGCAGAGA AATCTTGCGG ATCACAGACC CAAAAATGAC ATGTTACATA 
GACCAGCTGA ACACTTGGTA TGACGTGAAA ACTCATCAAG AAGTGACCAG CAGTCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGGACT TTTGGTCTGA ATGGATTGGA CAGGCTTCTG 
TGCTTCATGA TTGTGAAAGA GCTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AAAGAGAGAA CTGTTCAAGA AACTTTAAAG ATGCTCATGA GTGCTGTTAA CCCTCTAAAG 
AGCATAGTGG CAAATTCAAG TAAAGTTTAT CTTTCTGCCA TCACCAAAAC ACAGAAGATC 
TGGAGTGCTT ACCTGGAGGC TATAATGAAG GTTGGACAGA TGCAGATCCT GAGACAACAG 
ATCGCCAATG AGCTAAACTC TTCCTGTCGA TTTGATTCCA GACATCTGGC AGCTGCCCTG 
GATAATCTCA ATAAGGCTCT CCTGGCAGAC ATCGAAGCCC ACTACCGGGA CCCTTCGCTT 
CCTTACCCCA AAGAAGATAA CACACTCTTA TATGAAATCA CAGCCTATCT GGAGGCGGCT 
GGCATTCACA ACCCCCTGAA TAAGATTTAC ATAACAACGA AGCGCTTACC CTATTTCCCG 
ATTGTCAACT TTCTGTTTTT GATCGCGCAG TTGCCAAAAC TTCAGTATAA CAAAAATCTA 
GGCATGGTCT GCCGGAAGCC TGCCGACCCT GTTGACTGGC CACCGCTCGT CCTAGGTCTG 
CTCACGCTGC TGAAGCAGTT CCACTCCCGG TACACCGAGC AATTCCTGGC ACTGATTGGC 
CAGTTTATTC GCTCCACAAT GGAGCAGTGC ACAAGCCAGA AGATGCCTGA AATGCCTGCA 
GATGCTGTGG GCGCCCTTCT CTTCCTGGAG GATTATGTTC GGTATACAAA ACTCCCCCGA 
AGGGTGGCTG AAGCCCATGT GCCTAACTTC ATTTTTGATG AATTCCGAAC AGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc5 ( XM_006520759.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520822.1 Mus musculus RIKEN cDNA E430025E21 gene (E430025E21Rik), transcript variant X1, mRNA.

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CloneID	OMu51164
Clone ID Related Accession (Same CDS sequence)	XM_006520759.3 , XM_006520759.4
Accession Version	XM_006520759.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit strumpellin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT CATCTTTGAC TTCAGCTACT TTAAGATACC TCGATGACCT CAATGAAGGA 
GTTTATATTC AGCAAACCTT AGAAACCGTG CTTCTCAGTG AAGATGGAAA ACAACTTCTA 
TGTGAAGCCC TGTACTTATA TGGAGTTATG CTGCTTGTCA TCGACCAGAA GATTGAAGGA 
GAAGTCAGAG AGAGAATGCT GGTTTCTTAC TATCGATACA GTGCTGCTCG GTCCTCTGCT 
GACTCAAACA TGGATGATAT CTGTAAGCTG CTACGAAGCA CAGGATATTC CAGCCAGCCA 
GGAGCCAAAA GACCACCCAA CTATCCTGAG AGCTATTTCC AGAGAGTGCC TATCAACGAG 
ACGTTCATCA GCATGGTCAT CGGACGCCTG AGGTCTGATG ACATTTACAA CCAGGTCTCT 
GCCTACCCGC TGCCGGAGCA CCGCAGCACT GCCCTGGCCA ATCAAGCCGC CATGCTCTAC 
GTGATTCTCT ATTTTGAACC TTCCATTCTT CACACCCACC AAGCAAAAAT GAGAGAGATC 
GTGGATAAGT ACTTTCCAGA TAACTGGGTA ATCAGCATCT ATATGGGTAT CACTGTTAAT 
CTAGCTGATG CCTGGGAACC TTACAAAGCT GCGAAAACTG CTCTCAACAA CACCCTTGAC 
CTCGCCAATG TCAAAGAACA GGCAAGCAGA TACGCTAGTG TCAGCGACAG AGTGCGCGCT 
CAGGTGCAGC AGTTTCTGAA AGAGGGCTAC CTGAGGGAGG AGGTGCTTCT GGACAACATC 
CCCAGGCTGC TGAACTGCCT GAGAGACTGC AATGTCGCCA TCCGCTGGCT AATGCTCCAC 
ACAGCCGACT CAGCCTGTGA CCCAAACAAC AAGCGCCTTC GTCAAATCAA GGACCAGATT 
CTGGCAGACT CTAGGTACAA TCCCAAGATC CTCTTCCAGT TGCTATTGGA CACTGCACAA 
TTCGAGTTTA TACTCAAAGA GATGTTCAAG CAAATGCTTT CCGAGAAACA GTCCAAGTGG 
GAGCATTACA AGAAGGAGGG CTCTGAGCGC ATGACCGAGC TTGCTGACGT CTTTTCAGGG 
GTGAAACCAC TAACCCGAGT GGAGAAAAAC GAAAATCTTC AAGCTTGGTT CAGAGAGATC 
TCAAAACAAA TACTGTCTCT AAATTATGAT GATTCTACTG CTGCAGGCAG AAAAACTGTG 
CAACTAATAC AAGCTCTGGA AGAGGTTCAG GAGTTTCACC AGTTGGAATC AAATCTACAA 
GTTTGCCAGT TTCTAGCTGA CACCCGAAAG TTTCTCCATC AAATGATCAG AACCATCAAC 
ATCAAGGAGG AAGTCCTGAT CACAGTGCAG ATCATCGGGG ACCTTTCCTT TGCCTGGCAG 
TTAATTGACA GTTTCACATC CATCATGCAA GAAAGCATAA GGGTAAACCC ATCCATGGTT 
ACCAAACTCA GAGCCACGTT CCTAAAGCTT GCCTCTGCCC TTGACCTTCC CCTGCTACGT 
ATTAATCAAG CAAATAGCCC TGATCTCCTC AGTGTGTCTC AGTATTATTC AGGAGAATTG 
GTGTCATACG TGAGAAAGGT TTTGCAGATC ATTCCAGAAA GCATGTTCAC ATCTCTCCTG 
AAGATCATAA AGCTTCAGAC CCATGACATC ATGGAAGTGC CTACACGCCT GGACAAGGAC 
AAGCTGCGGG ACTACGCTCA GCTTGGCCCG AGATATGAGG TTGCCAAGCT CACTCACGCC 
ATCTCCATCT TTACTGAGGG CATCTTGATG ATGAAAACGA CCCTGGTGGG CATCATCAAG 
GTGGATCCCA AGCAGTTGCT GGAAGACGGA ATAAGGAAAG AGCTGGTTAA ACGCGTGGCT 
TTCGCCCTTC ACCGGGGACT GATATTCAAC CCTCGTGCCA AGCCAAGTGA GTTGATGCCC 
AAACTGAAAG AGCTGGGGGC CACCATGGAT GGGTTCCATC GCTCCTTTGA GTATATACAG 
GACTACGTCA GCATTTACGG TCTGAAGATT TGGCAGGAGG AAGTCTCTCG TATTATAAAT 
TATAACGTGG AACAGGAGTG TAATAACTTC TTAAGAACAA AGATTCAAGA CTGGCAGAGT 
ATGTACCAAT CCACTCATAT ACCAATCCCG AAGTTTGCAC CTGTGGATGA GTCTATAACA 
TTTATTGGTC GACTCTGCAG AGAAATCTTG CGGATCACAG ACCCAAAAAT GACATGTTAC 
ATAGACCAGC TGAACACTTG GTATGACGTG AAAACTCATC AAGAAGTGAC CAGCAGTCGC 
CTCTTCTCAG AAATCCAGAC CACCTTGGGG ACTTTTGGTC TGAATGGATT GGACAGGCTT 
CTGTGCTTCA TGATTGTGAA AGAGCTACAG AATTTCCTCA GTATGTTTCA GAAAATTATC 
CTGAAAGAGA GAACTGTTCA AGAAACTTTA AAGATGCTCA TGAGTGCTGT TAACCCTCTA 
AAGAGCATAG TGGCAAATTC AAGTAAAGTT TATCTTTCTG CCATCACCAA AACACAGAAG 
ATCTGGAGTG CTTACCTGGA GGCTATAATG AAGGTTGGAC AGATGCAGAT CCTGAGACAA 
CAGATCGCCA ATGAGCTAAA CTCTTCCTGT CGATTTGATT CCAGACATCT GGCAGCTGCC 
CTGGATAATC TCAATAAGGC TCTCCTGGCA GACATCGAAG CCCACTACCG GGACCCTTCG 
CTTCCTTACC CCAAAGAAGA TAACACACTC TTATATGAAA TCACAGCCTA TCTGGAGGCG 
GCTGGCATTC ACAACCCCCT GAATAAGATT TACATAACAA CGAAGCGCTT ACCCTATTTC 
CCGATTGTCA ACTTTCTGTT TTTGATCGCG CAGTTGCCAA AACTTCAGTA TAACAAAAAT 
CTAGGCATGG TCTGCCGGAA GCCTGCCGAC CCTGTTGACT GGCCACCGCT CGTCCTAGGT 
CTGCTCACGC TGCTGAAGCA GTTCCACTCC CGGTACACCG AGCAATTCCT GGCACTGATT 
GGCCAGTTTA TTCGCTCCAC AATGGAGCAG TGCACAAGCC AGAAGATGCC TGAAATGCCT 
GCAGATGCTG TGGGCGCCCT TCTCTTCCTG GAGGATTATG TTCGGTATAC AAAACTCCCC 
CGAAGGGTGG CTGAAGCCCA TGTGCCTAAC TTCATTTTTG ATGAATTCCG AACAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520822.1
CDS	428..3610
Translation	METSSLTSATLRYLDDLNEGVYIQQTLETVLLSEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPPNYPESYFQRVPINETFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLANVKEQASRYASVSDRVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMSAVNPLKSIVANSSKVYLSAITKTQKIWSAYLEAIMKVGQMQILRQQIANELNSSCRFDSRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	XM_006520759.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIKEN cDNA E430025E21 gene (E430025E21Rik), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520759.3

ORF Insert Sequence:

ATGGAGACAT CATCTTTGAC TTCAGCTACT TTAAGATACC TCGATGACCT CAATGAAGGA 
GTTTATATTC AGCAAACCTT AGAAACCGTG CTTCTCAGTG AAGATGGAAA ACAACTTCTA 
TGTGAAGCCC TGTACTTATA TGGAGTTATG CTGCTTGTCA TCGACCAGAA GATTGAAGGA 
GAAGTCAGAG AGAGAATGCT GGTTTCTTAC TATCGATACA GTGCTGCTCG GTCCTCTGCT 
GACTCAAACA TGGATGATAT CTGTAAGCTG CTACGAAGCA CAGGATATTC CAGCCAGCCA 
GGAGCCAAAA GACCACCCAA CTATCCTGAG AGCTATTTCC AGAGAGTGCC TATCAACGAG 
ACGTTCATCA GCATGGTCAT CGGACGCCTG AGGTCTGATG ACATTTACAA CCAGGTCTCT 
GCCTACCCGC TGCCGGAGCA CCGCAGCACT GCCCTGGCCA ATCAAGCCGC CATGCTCTAC 
GTGATTCTCT ATTTTGAACC TTCCATTCTT CACACCCACC AAGCAAAAAT GAGAGAGATC 
GTGGATAAGT ACTTTCCAGA TAACTGGGTA ATCAGCATCT ATATGGGTAT CACTGTTAAT 
CTAGCTGATG CCTGGGAACC TTACAAAGCT GCGAAAACTG CTCTCAACAA CACCCTTGAC 
CTCGCCAATG TCAAAGAACA GGCAAGCAGA TACGCTAGTG TCAGCGACAG AGTGCGCGCT 
CAGGTGCAGC AGTTTCTGAA AGAGGGCTAC CTGAGGGAGG AGGTGCTTCT GGACAACATC 
CCCAGGCTGC TGAACTGCCT GAGAGACTGC AATGTCGCCA TCCGCTGGCT AATGCTCCAC 
ACAGCCGACT CAGCCTGTGA CCCAAACAAC AAGCGCCTTC GTCAAATCAA GGACCAGATT 
CTGGCAGACT CTAGGTACAA TCCCAAGATC CTCTTCCAGT TGCTATTGGA CACTGCACAA 
TTCGAGTTTA TACTCAAAGA GATGTTCAAG CAAATGCTTT CCGAGAAACA GTCCAAGTGG 
GAGCATTACA AGAAGGAGGG CTCTGAGCGC ATGACCGAGC TTGCTGACGT CTTTTCAGGG 
GTGAAACCAC TAACCCGAGT GGAGAAAAAC GAAAATCTTC AAGCTTGGTT CAGAGAGATC 
TCAAAACAAA TACTGTCTCT AAATTATGAT GATTCTACTG CTGCAGGCAG AAAAACTGTG 
CAACTAATAC AAGCTCTGGA AGAGGTTCAG GAGTTTCACC AGTTGGAATC AAATCTACAA 
GTTTGCCAGT TTCTAGCTGA CACCCGAAAG TTTCTCCATC AAATGATCAG AACCATCAAC 
ATCAAGGAGG AAGTCCTGAT CACAGTGCAG ATCATCGGGG ACCTTTCCTT TGCCTGGCAG 
TTAATTGACA GTTTCACATC CATCATGCAA GAAAGCATAA GGGTAAACCC ATCCATGGTT 
ACCAAACTCA GAGCCACGTT CCTAAAGCTT GCCTCTGCCC TTGACCTTCC CCTGCTACGT 
ATTAATCAAG CAAATAGCCC TGATCTCCTC AGTGTGTCTC AGTATTATTC AGGAGAATTG 
GTGTCATACG TGAGAAAGGT TTTGCAGATC ATTCCAGAAA GCATGTTCAC ATCTCTCCTG 
AAGATCATAA AGCTTCAGAC CCATGACATC ATGGAAGTGC CTACACGCCT GGACAAGGAC 
AAGCTGCGGG ACTACGCTCA GCTTGGCCCG AGATATGAGG TTGCCAAGCT CACTCACGCC 
ATCTCCATCT TTACTGAGGG CATCTTGATG ATGAAAACGA CCCTGGTGGG CATCATCAAG 
GTGGATCCCA AGCAGTTGCT GGAAGACGGA ATAAGGAAAG AGCTGGTTAA ACGCGTGGCT 
TTCGCCCTTC ACCGGGGACT GATATTCAAC CCTCGTGCCA AGCCAAGTGA GTTGATGCCC 
AAACTGAAAG AGCTGGGGGC CACCATGGAT GGGTTCCATC GCTCCTTTGA GTATATACAG 
GACTACGTCA GCATTTACGG TCTGAAGATT TGGCAGGAGG AAGTCTCTCG TATTATAAAT 
TATAACGTGG AACAGGAGTG TAATAACTTC TTAAGAACAA AGATTCAAGA CTGGCAGAGT 
ATGTACCAAT CCACTCATAT ACCAATCCCG AAGTTTGCAC CTGTGGATGA GTCTATAACA 
TTTATTGGTC GACTCTGCAG AGAAATCTTG CGGATCACAG ACCCAAAAAT GACATGTTAC 
ATAGACCAGC TGAACACTTG GTATGACGTG AAAACTCATC AAGAAGTGAC CAGCAGTCGC 
CTCTTCTCAG AAATCCAGAC CACCTTGGGG ACTTTTGGTC TGAATGGATT GGACAGGCTT 
CTGTGCTTCA TGATTGTGAA AGAGCTACAG AATTTCCTCA GTATGTTTCA GAAAATTATC 
CTGAAAGAGA GAACTGTTCA AGAAACTTTA AAGATGCTCA TGAGTGCTGT TAACCCTCTA 
AAGAGCATAG TGGCAAATTC AAGTAAAGTT TATCTTTCTG CCATCACCAA AACACAGAAG 
ATCTGGAGTG CTTACCTGGA GGCTATAATG AAGGTTGGAC AGATGCAGAT CCTGAGACAA 
CAGATCGCCA ATGAGCTAAA CTCTTCCTGT CGATTTGATT CCAGACATCT GGCAGCTGCC 
CTGGATAATC TCAATAAGGC TCTCCTGGCA GACATCGAAG CCCACTACCG GGACCCTTCG 
CTTCCTTACC CCAAAGAAGA TAACACACTC TTATATGAAA TCACAGCCTA TCTGGAGGCG 
GCTGGCATTC ACAACCCCCT GAATAAGATT TACATAACAA CGAAGCGCTT ACCCTATTTC 
CCGATTGTCA ACTTTCTGTT TTTGATCGCG CAGTTGCCAA AACTTCAGTA TAACAAAAAT 
CTAGGCATGG TCTGCCGGAA GCCTGCCGAC CCTGTTGACT GGCCACCGCT CGTCCTAGGT 
CTGCTCACGC TGCTGAAGCA GTTCCACTCC CGGTACACCG AGCAATTCCT GGCACTGATT 
GGCCAGTTTA TTCGCTCCAC AATGGAGCAG TGCACAAGCC AGAAGATGCC TGAAATGCCT 
GCAGATGCTG TGGGCGCCCT TCTCTTCCTG GAGGATTATG TTCGGTATAC AAAACTCCCC 
CGAAGGGTGG CTGAAGCCCA TGTGCCTAAC TTCATTTTTG ATGAATTCCG AACAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc5 ( XM_006520759.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520822.1 Mus musculus WASH complex subunit 5 (Washc5), transcript variant X1, mRNA.

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CloneID	OMu51164
Clone ID Related Accession (Same CDS sequence)	XM_006520759.3 , XM_006520759.4
Accession Version	XM_006520759.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520759.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT CATCTTTGAC TTCAGCTACT TTAAGATACC TCGATGACCT CAATGAAGGA 
GTTTATATTC AGCAAACCTT AGAAACCGTG CTTCTCAGTG AAGATGGAAA ACAACTTCTA 
TGTGAAGCCC TGTACTTATA TGGAGTTATG CTGCTTGTCA TCGACCAGAA GATTGAAGGA 
GAAGTCAGAG AGAGAATGCT GGTTTCTTAC TATCGATACA GTGCTGCTCG GTCCTCTGCT 
GACTCAAACA TGGATGATAT CTGTAAGCTG CTACGAAGCA CAGGATATTC CAGCCAGCCA 
GGAGCCAAAA GACCACCCAA CTATCCTGAG AGCTATTTCC AGAGAGTGCC TATCAACGAG 
ACGTTCATCA GCATGGTCAT CGGACGCCTG AGGTCTGATG ACATTTACAA CCAGGTCTCT 
GCCTACCCGC TGCCGGAGCA CCGCAGCACT GCCCTGGCCA ATCAAGCCGC CATGCTCTAC 
GTGATTCTCT ATTTTGAACC TTCCATTCTT CACACCCACC AAGCAAAAAT GAGAGAGATC 
GTGGATAAGT ACTTTCCAGA TAACTGGGTA ATCAGCATCT ATATGGGTAT CACTGTTAAT 
CTAGCTGATG CCTGGGAACC TTACAAAGCT GCGAAAACTG CTCTCAACAA CACCCTTGAC 
CTCGCCAATG TCAAAGAACA GGCAAGCAGA TACGCTAGTG TCAGCGACAG AGTGCGCGCT 
CAGGTGCAGC AGTTTCTGAA AGAGGGCTAC CTGAGGGAGG AGGTGCTTCT GGACAACATC 
CCCAGGCTGC TGAACTGCCT GAGAGACTGC AATGTCGCCA TCCGCTGGCT AATGCTCCAC 
ACAGCCGACT CAGCCTGTGA CCCAAACAAC AAGCGCCTTC GTCAAATCAA GGACCAGATT 
CTGGCAGACT CTAGGTACAA TCCCAAGATC CTCTTCCAGT TGCTATTGGA CACTGCACAA 
TTCGAGTTTA TACTCAAAGA GATGTTCAAG CAAATGCTTT CCGAGAAACA GTCCAAGTGG 
GAGCATTACA AGAAGGAGGG CTCTGAGCGC ATGACCGAGC TTGCTGACGT CTTTTCAGGG 
GTGAAACCAC TAACCCGAGT GGAGAAAAAC GAAAATCTTC AAGCTTGGTT CAGAGAGATC 
TCAAAACAAA TACTGTCTCT AAATTATGAT GATTCTACTG CTGCAGGCAG AAAAACTGTG 
CAACTAATAC AAGCTCTGGA AGAGGTTCAG GAGTTTCACC AGTTGGAATC AAATCTACAA 
GTTTGCCAGT TTCTAGCTGA CACCCGAAAG TTTCTCCATC AAATGATCAG AACCATCAAC 
ATCAAGGAGG AAGTCCTGAT CACAGTGCAG ATCATCGGGG ACCTTTCCTT TGCCTGGCAG 
TTAATTGACA GTTTCACATC CATCATGCAA GAAAGCATAA GGGTAAACCC ATCCATGGTT 
ACCAAACTCA GAGCCACGTT CCTAAAGCTT GCCTCTGCCC TTGACCTTCC CCTGCTACGT 
ATTAATCAAG CAAATAGCCC TGATCTCCTC AGTGTGTCTC AGTATTATTC AGGAGAATTG 
GTGTCATACG TGAGAAAGGT TTTGCAGATC ATTCCAGAAA GCATGTTCAC ATCTCTCCTG 
AAGATCATAA AGCTTCAGAC CCATGACATC ATGGAAGTGC CTACACGCCT GGACAAGGAC 
AAGCTGCGGG ACTACGCTCA GCTTGGCCCG AGATATGAGG TTGCCAAGCT CACTCACGCC 
ATCTCCATCT TTACTGAGGG CATCTTGATG ATGAAAACGA CCCTGGTGGG CATCATCAAG 
GTGGATCCCA AGCAGTTGCT GGAAGACGGA ATAAGGAAAG AGCTGGTTAA ACGCGTGGCT 
TTCGCCCTTC ACCGGGGACT GATATTCAAC CCTCGTGCCA AGCCAAGTGA GTTGATGCCC 
AAACTGAAAG AGCTGGGGGC CACCATGGAT GGGTTCCATC GCTCCTTTGA GTATATACAG 
GACTACGTCA GCATTTACGG TCTGAAGATT TGGCAGGAGG AAGTCTCTCG TATTATAAAT 
TATAACGTGG AACAGGAGTG TAATAACTTC TTAAGAACAA AGATTCAAGA CTGGCAGAGT 
ATGTACCAAT CCACTCATAT ACCAATCCCG AAGTTTGCAC CTGTGGATGA GTCTATAACA 
TTTATTGGTC GACTCTGCAG AGAAATCTTG CGGATCACAG ACCCAAAAAT GACATGTTAC 
ATAGACCAGC TGAACACTTG GTATGACGTG AAAACTCATC AAGAAGTGAC CAGCAGTCGC 
CTCTTCTCAG AAATCCAGAC CACCTTGGGG ACTTTTGGTC TGAATGGATT GGACAGGCTT 
CTGTGCTTCA TGATTGTGAA AGAGCTACAG AATTTCCTCA GTATGTTTCA GAAAATTATC 
CTGAAAGAGA GAACTGTTCA AGAAACTTTA AAGATGCTCA TGAGTGCTGT TAACCCTCTA 
AAGAGCATAG TGGCAAATTC AAGTAAAGTT TATCTTTCTG CCATCACCAA AACACAGAAG 
ATCTGGAGTG CTTACCTGGA GGCTATAATG AAGGTTGGAC AGATGCAGAT CCTGAGACAA 
CAGATCGCCA ATGAGCTAAA CTCTTCCTGT CGATTTGATT CCAGACATCT GGCAGCTGCC 
CTGGATAATC TCAATAAGGC TCTCCTGGCA GACATCGAAG CCCACTACCG GGACCCTTCG 
CTTCCTTACC CCAAAGAAGA TAACACACTC TTATATGAAA TCACAGCCTA TCTGGAGGCG 
GCTGGCATTC ACAACCCCCT GAATAAGATT TACATAACAA CGAAGCGCTT ACCCTATTTC 
CCGATTGTCA ACTTTCTGTT TTTGATCGCG CAGTTGCCAA AACTTCAGTA TAACAAAAAT 
CTAGGCATGG TCTGCCGGAA GCCTGCCGAC CCTGTTGACT GGCCACCGCT CGTCCTAGGT 
CTGCTCACGC TGCTGAAGCA GTTCCACTCC CGGTACACCG AGCAATTCCT GGCACTGATT 
GGCCAGTTTA TTCGCTCCAC AATGGAGCAG TGCACAAGCC AGAAGATGCC TGAAATGCCT 
GCAGATGCTG TGGGCGCCCT TCTCTTCCTG GAGGATTATG TTCGGTATAC AAAACTCCCC 
CGAAGGGTGG CTGAAGCCCA TGTGCCTAAC TTCATTTTTG ATGAATTCCG AACAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520822.1
CDS	426..3608
Translation	METSSLTSATLRYLDDLNEGVYIQQTLETVLLSEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPPNYPESYFQRVPINETFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLANVKEQASRYASVSDRVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMSAVNPLKSIVANSSKVYLSAITKTQKIWSAYLEAIMKVGQMQILRQQIANELNSSCRFDSRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	XM_006520759.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 5 (Washc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520759.4

ORF Insert Sequence:

ATGGAGACAT CATCTTTGAC TTCAGCTACT TTAAGATACC TCGATGACCT CAATGAAGGA 
GTTTATATTC AGCAAACCTT AGAAACCGTG CTTCTCAGTG AAGATGGAAA ACAACTTCTA 
TGTGAAGCCC TGTACTTATA TGGAGTTATG CTGCTTGTCA TCGACCAGAA GATTGAAGGA 
GAAGTCAGAG AGAGAATGCT GGTTTCTTAC TATCGATACA GTGCTGCTCG GTCCTCTGCT 
GACTCAAACA TGGATGATAT CTGTAAGCTG CTACGAAGCA CAGGATATTC CAGCCAGCCA 
GGAGCCAAAA GACCACCCAA CTATCCTGAG AGCTATTTCC AGAGAGTGCC TATCAACGAG 
ACGTTCATCA GCATGGTCAT CGGACGCCTG AGGTCTGATG ACATTTACAA CCAGGTCTCT 
GCCTACCCGC TGCCGGAGCA CCGCAGCACT GCCCTGGCCA ATCAAGCCGC CATGCTCTAC 
GTGATTCTCT ATTTTGAACC TTCCATTCTT CACACCCACC AAGCAAAAAT GAGAGAGATC 
GTGGATAAGT ACTTTCCAGA TAACTGGGTA ATCAGCATCT ATATGGGTAT CACTGTTAAT 
CTAGCTGATG CCTGGGAACC TTACAAAGCT GCGAAAACTG CTCTCAACAA CACCCTTGAC 
CTCGCCAATG TCAAAGAACA GGCAAGCAGA TACGCTAGTG TCAGCGACAG AGTGCGCGCT 
CAGGTGCAGC AGTTTCTGAA AGAGGGCTAC CTGAGGGAGG AGGTGCTTCT GGACAACATC 
CCCAGGCTGC TGAACTGCCT GAGAGACTGC AATGTCGCCA TCCGCTGGCT AATGCTCCAC 
ACAGCCGACT CAGCCTGTGA CCCAAACAAC AAGCGCCTTC GTCAAATCAA GGACCAGATT 
CTGGCAGACT CTAGGTACAA TCCCAAGATC CTCTTCCAGT TGCTATTGGA CACTGCACAA 
TTCGAGTTTA TACTCAAAGA GATGTTCAAG CAAATGCTTT CCGAGAAACA GTCCAAGTGG 
GAGCATTACA AGAAGGAGGG CTCTGAGCGC ATGACCGAGC TTGCTGACGT CTTTTCAGGG 
GTGAAACCAC TAACCCGAGT GGAGAAAAAC GAAAATCTTC AAGCTTGGTT CAGAGAGATC 
TCAAAACAAA TACTGTCTCT AAATTATGAT GATTCTACTG CTGCAGGCAG AAAAACTGTG 
CAACTAATAC AAGCTCTGGA AGAGGTTCAG GAGTTTCACC AGTTGGAATC AAATCTACAA 
GTTTGCCAGT TTCTAGCTGA CACCCGAAAG TTTCTCCATC AAATGATCAG AACCATCAAC 
ATCAAGGAGG AAGTCCTGAT CACAGTGCAG ATCATCGGGG ACCTTTCCTT TGCCTGGCAG 
TTAATTGACA GTTTCACATC CATCATGCAA GAAAGCATAA GGGTAAACCC ATCCATGGTT 
ACCAAACTCA GAGCCACGTT CCTAAAGCTT GCCTCTGCCC TTGACCTTCC CCTGCTACGT 
ATTAATCAAG CAAATAGCCC TGATCTCCTC AGTGTGTCTC AGTATTATTC AGGAGAATTG 
GTGTCATACG TGAGAAAGGT TTTGCAGATC ATTCCAGAAA GCATGTTCAC ATCTCTCCTG 
AAGATCATAA AGCTTCAGAC CCATGACATC ATGGAAGTGC CTACACGCCT GGACAAGGAC 
AAGCTGCGGG ACTACGCTCA GCTTGGCCCG AGATATGAGG TTGCCAAGCT CACTCACGCC 
ATCTCCATCT TTACTGAGGG CATCTTGATG ATGAAAACGA CCCTGGTGGG CATCATCAAG 
GTGGATCCCA AGCAGTTGCT GGAAGACGGA ATAAGGAAAG AGCTGGTTAA ACGCGTGGCT 
TTCGCCCTTC ACCGGGGACT GATATTCAAC CCTCGTGCCA AGCCAAGTGA GTTGATGCCC 
AAACTGAAAG AGCTGGGGGC CACCATGGAT GGGTTCCATC GCTCCTTTGA GTATATACAG 
GACTACGTCA GCATTTACGG TCTGAAGATT TGGCAGGAGG AAGTCTCTCG TATTATAAAT 
TATAACGTGG AACAGGAGTG TAATAACTTC TTAAGAACAA AGATTCAAGA CTGGCAGAGT 
ATGTACCAAT CCACTCATAT ACCAATCCCG AAGTTTGCAC CTGTGGATGA GTCTATAACA 
TTTATTGGTC GACTCTGCAG AGAAATCTTG CGGATCACAG ACCCAAAAAT GACATGTTAC 
ATAGACCAGC TGAACACTTG GTATGACGTG AAAACTCATC AAGAAGTGAC CAGCAGTCGC 
CTCTTCTCAG AAATCCAGAC CACCTTGGGG ACTTTTGGTC TGAATGGATT GGACAGGCTT 
CTGTGCTTCA TGATTGTGAA AGAGCTACAG AATTTCCTCA GTATGTTTCA GAAAATTATC 
CTGAAAGAGA GAACTGTTCA AGAAACTTTA AAGATGCTCA TGAGTGCTGT TAACCCTCTA 
AAGAGCATAG TGGCAAATTC AAGTAAAGTT TATCTTTCTG CCATCACCAA AACACAGAAG 
ATCTGGAGTG CTTACCTGGA GGCTATAATG AAGGTTGGAC AGATGCAGAT CCTGAGACAA 
CAGATCGCCA ATGAGCTAAA CTCTTCCTGT CGATTTGATT CCAGACATCT GGCAGCTGCC 
CTGGATAATC TCAATAAGGC TCTCCTGGCA GACATCGAAG CCCACTACCG GGACCCTTCG 
CTTCCTTACC CCAAAGAAGA TAACACACTC TTATATGAAA TCACAGCCTA TCTGGAGGCG 
GCTGGCATTC ACAACCCCCT GAATAAGATT TACATAACAA CGAAGCGCTT ACCCTATTTC 
CCGATTGTCA ACTTTCTGTT TTTGATCGCG CAGTTGCCAA AACTTCAGTA TAACAAAAAT 
CTAGGCATGG TCTGCCGGAA GCCTGCCGAC CCTGTTGACT GGCCACCGCT CGTCCTAGGT 
CTGCTCACGC TGCTGAAGCA GTTCCACTCC CGGTACACCG AGCAATTCCT GGCACTGATT 
GGCCAGTTTA TTCGCTCCAC AATGGAGCAG TGCACAAGCC AGAAGATGCC TGAAATGCCT 
GCAGATGCTG TGGGCGCCCT TCTCTTCCTG GAGGATTATG TTCGGTATAC AAAACTCCCC 
CGAAGGGTGG CTGAAGCCCA TGTGCCTAAC TTCATTTTTG ATGAATTCCG AACAGTCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of N471D strumpellin leads to defects in the endolysosomal system.
Disease models & mechanisms11(9)(2018 09)
Song L,Rijal R,Karow M,Stumpf M,Hahn O,Park L,Insall R,Schröder R,Hofmann A,Clemen CS,Eichinger L

Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.
Pigment cell & melanoma research29(5)559-71(2016 09)
Tyrrell BJ,Woodham EF,Spence HJ,Strathdee D,Insall RH,Machesky LM

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
Nature communications710961(2016 Mar)
Bartuzi P,Billadeau DD,Favier R,Rong S,Dekker D,Fedoseienko A,Fieten H,Wijers M,Levels JH,Huijkman N,Kloosterhuis N,van der Molen H,Brufau G,Groen AK,Elliott AM,Kuivenhoven JA,Plecko B,Grangl G,McGaughran J,Horton JD,Burstein E,Hofker MH,van de Sluis B

The spectrum of KIAA0196 variants, and characterization of a murine knockout: implications for the mutational mechanism in hereditary spastic paraplegia type SPG8.
Orphanet journal of rare diseases10147(2015 Nov)
Jahic A,Khundadze M,Jaenisch N,Schüle R,Klimpe S,Klebe S,Frahm C,Kassubek J,Stevanin G,Schöls L,Brice A,Hübner CA,Beetz C

WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes.
Scientific reports45596(2014 Jul)
Wang F,Zhang L,Zhang GL,Wang ZB,Cui XS,Kim NH,Sun SC

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Washc5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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