Ube2h cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2h
Entrez Gene ID	22214
Full Name	ubiquitin-conjugating enzyme E2H
Synonyms	1500009C23Rik,AI181839,AI326965,AI462483,AW227540,E2-20K,Gid3,N28148,Ubc8
General protein information	Preferred Names ubiquitin-conjugating enzyme E2H Names ubiquitin-conjugating enzyme E2 H (E3-independent) E2 ubiquitin-conjugating enzyme H E2 ubiquitin-conjugating enzyme H UBCH2 ubiquitin carrier protein H ubiquitin-conjugating enzyme E2-20K ubiquitin-protein ligase H
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A3.3\|6 12.52 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321519.1	XP_017177008.1	ubiquitin-conjugating enzyme E2 H isoform X1
NM_009459.3	NP_033485.1	ubiquitin-conjugating enzyme E2 H isoform 1
NM_001169576.1	NP_001163047.1	ubiquitin-conjugating enzyme E2 H isoform 2
NM_001169577.1	NP_001163048.1	ubiquitin-conjugating enzyme E2 H isoform 3
XM_017321519.2	XP_017177008.1	ubiquitin-conjugating enzyme E2 H isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2H	NP_003335.1
Pan troglodytes (chimpanzee)	UBE2H	XP_001144807.2
Gallus gallus (chicken)	UBE2H	NP_001025818.1
Danio rerio (zebrafish)	ube2h	NP_958897.1
Drosophila melanogaster (fruit fly)	Ubc-E2H	NP_727234.1
Caenorhabditis elegans (roundworm)	ubc-8	NP_500245.2
Schizosaccharomyces pombe (fission yeast)	ubc8	NP_596617.1
Neurospora crassa	NCU10046	XP_957953.1
Bos taurus (cattle)	UBE2H	NP_001032703.1
Rattus norvegicus (Norway rat)	Ube2h	NP_001165598.1
Arabidopsis thaliana (thale cress)	UBC5	NP_564817.2
Arabidopsis thaliana (thale cress)	UBC4	NP_568589.1
Magnaporthe oryzae (rice blast fungus)	MGG_00600	XP_003718439.1
Xenopus tropicalis (tropical clawed frog)	ube2h	NP_001004909.1
Eremothecium gossypii	AGOS_AEL045W	NP_984816.1
Macaca mulatta (Rhesus monkey)	UBE2H	XP_001093822.1
Canis lupus familiaris (dog)	UBE2H	XP_857419.1
Arabidopsis thaliana (thale cress)	UBC6	NP_566062.1
Oryza sativa (rice)	Os10g0447100	NP_001064719.1
Kluyveromyces lactis	KLLA0A11198g	XP_451487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001068	XP_318292.1
Mus musculus (house mouse)	Ube2h	NP_033485.1
Saccharomyces cerevisiae (baker's yeast)	UBC8	NP_010904.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

MG53-induced IRS-1 ubiquitination negatively regulates skeletal myogenesis and insulin signalling.
Yi JS, Park JS, Ham YM, Nguyen N, Lee NR, Hong J, Kim BW, Lee H, Lee CS, Jeong BC, Song HK, Cho H, Kim YK, Lee JS, Park KS, Shin H, Choi I, Lee SH, Park WJ, Park SY, Choi CS, Lin P, Karunasiri M, Tan T, Duann P, Zhu H, Ma J, Ko YG
Nature communications42354(2013)

Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation.
Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE
Molecular and cellular biology24(21)9592-600(2004 Nov)

Human-mouse comparative mapping of the genomic region containing CDK6: localization of an evolutionary breakpoint.
Thomas JW, Lee-Lin SQ, Green ED
Mammalian genome : official journal of the International Mammalian Genome Society10(7)764-7(1999 Jul)

Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis.
Brady KP, Rowe LB, Her H, Stevens TJ, Eppig J, Sussman DJ, Sikela J, Beier DR
Genome research7(11)1085-93(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17799	NM_001169577.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu17836	NM_001169576.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu17799	XM_017321519.2 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu17747	NM_009459.3 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OMu17799	XM_017321519.1	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2h ( NM_001169577.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001163048.1 Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17799
Clone ID Related Accession (Same CDS sequence)	XM_017321519.1 , NM_001169577.1 , XM_017321519.2
Accession Version	NM_001169577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 H isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161538.7. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BI150827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163048.1
CDS	129..470
Translation	MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_001169577.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169577.1

ORF Insert Sequence:

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2h ( NM_001169576.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001163047.1 Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17836
Clone ID Related Accession (Same CDS sequence)	NM_001169576.1
Accession Version	NM_001169576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 H isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF539343.1, AK161414.1, AK131688.1 and AC161538.7. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK161414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCCC CCAGTCCCGG CAAGAGGCGG ATGGACACGG ACGTAGTCAA GCTCATTGAA 
AGCAAACATG AGGTTACAAT CCTAGGAGGA CTCAATGAAT TCGTTGTCAA GTTTTACGGA 
CCACAAGGAA CACCATATGA AGGCGGGGTG TGGAAAGTGA GAGTGGACCT TCCTGATAAA 
TACCCTTTCA AGTCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCCCAG 
TTGCTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAGTACAA GCAGAAAATT AAAGAGTACA TCCAGAAGTA CGCAACGGAA 
GAGGCCCTGA AGGAACAGGA AGAGGGCACT GGCGACAGCT CATCGGAGAG TTCTATGTCT 
GACTTCTCAG AAGATGAGGC CCAGGACATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163047.1
CDS	436..894
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_001169576.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169576.1

ORF Insert Sequence:

ATGTCATCCC CCAGTCCCGG CAAGAGGCGG ATGGACACGG ACGTAGTCAA GCTCATTGAA 
AGCAAACATG AGGTTACAAT CCTAGGAGGA CTCAATGAAT TCGTTGTCAA GTTTTACGGA 
CCACAAGGAA CACCATATGA AGGCGGGGTG TGGAAAGTGA GAGTGGACCT TCCTGATAAA 
TACCCTTTCA AGTCTCCATC TATAGATCTT ACCAATATAT TTGAGTCCTT CCTGCCCCAG 
TTGCTGGCCT ATCCTAACCC CATAGATCCT CTCAATGGTG ACGCTGCAGC CATGTACCTC 
CACCGACCAG AAGAGTACAA GCAGAAAATT AAAGAGTACA TCCAGAAGTA CGCAACGGAA 
GAGGCCCTGA AGGAACAGGA AGAGGGCACT GGCGACAGCT CATCGGAGAG TTCTATGTCT 
GACTTCTCAG AAGATGAGGC CCAGGACATG GAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2h ( XM_017321519.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177008.1 Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17799
Clone ID Related Accession (Same CDS sequence)	XM_017321519.1 , NM_001169577.1 , XM_017321519.2
Accession Version	XM_017321519.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177008.1
CDS	1223..1564
Translation	MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	XM_017321519.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321519.1

ORF Insert Sequence:

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2h ( XM_017321519.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177008.1 Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17799
Clone ID Related Accession (Same CDS sequence)	XM_017321519.1 , NM_001169577.1 , XM_017321519.2
Accession Version	XM_017321519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177008.1
CDS	1222..1563
Translation	MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	XM_017321519.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321519.2

ORF Insert Sequence:

ATGAATAAAA TTTTCCATCC CAACATTGAT GAAGCGTCAG GAACTGTGTG TCTAGATGTA 
ATTAATCAAA CTTGGACAGC TCTCTATGAT CTTACCAATA TATTTGAGTC CTTCCTGCCC 
CAGTTGCTGG CCTATCCTAA CCCCATAGAT CCTCTCAATG GTGACGCTGC AGCCATGTAC 
CTCCACCGAC CAGAAGAGTA CAAGCAGAAA ATTAAAGAGT ACATCCAGAA GTACGCAACG 
GAAGAGGCCC TGAAGGAACA GGAAGAGGGC ACTGGCGACA GCTCATCGGA GAGTTCTATG 
TCTGACTTCT CAGAAGATGA GGCCCAGGAC ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2h ( NM_009459.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033485.1 Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17747
Clone ID Related Accession (Same CDS sequence)	NM_009459.3
Accession Version	NM_009459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 H isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF539343.1, AK131688.1 and AC161538.7. On Dec 17, 2009 this sequence version replaced NM_009459.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.36562.1, SRR1660819.31356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCCC CCAGTCCCGG CAAGAGGCGG ATGGACACGG ACGTAGTCAA GCTCATTGAA 
AGCAAACATG AGGTTACAAT CCTAGGAGGA CTCAATGAAT TCGTTGTCAA GTTTTACGGA 
CCACAAGGAA CACCATATGA AGGCGGGGTG TGGAAAGTGA GAGTGGACCT TCCTGATAAA 
TACCCTTTCA AGTCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCC CAGTTGCTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAGTA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA GTACGCAACG GAAGAGGCCC TGAAGGAACA GGAAGAGGGC 
ACTGGCGACA GCTCATCGGA GAGTTCTATG TCTGACTTCT CAGAAGATGA GGCCCAGGAC 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033485.1
CDS	436..987
Translation	MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Target ORF information:

RefSeq Version	NM_009459.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009459.3

ORF Insert Sequence:

ATGTCATCCC CCAGTCCCGG CAAGAGGCGG ATGGACACGG ACGTAGTCAA GCTCATTGAA 
AGCAAACATG AGGTTACAAT CCTAGGAGGA CTCAATGAAT TCGTTGTCAA GTTTTACGGA 
CCACAAGGAA CACCATATGA AGGCGGGGTG TGGAAAGTGA GAGTGGACCT TCCTGATAAA 
TACCCTTTCA AGTCTCCATC TATAGGATTC ATGAATAAAA TTTTCCATCC CAACATTGAT 
GAAGCGTCAG GAACTGTGTG TCTAGATGTA ATTAATCAAA CTTGGACAGC TCTCTATGAT 
CTTACCAATA TATTTGAGTC CTTCCTGCCC CAGTTGCTGG CCTATCCTAA CCCCATAGAT 
CCTCTCAATG GTGACGCTGC AGCCATGTAC CTCCACCGAC CAGAAGAGTA CAAGCAGAAA 
ATTAAAGAGT ACATCCAGAA GTACGCAACG GAAGAGGCCC TGAAGGAACA GGAAGAGGGC 
ACTGGCGACA GCTCATCGGA GAGTTCTATG TCTGACTTCT CAGAAGATGA GGCCCAGGAC 
ATGGAGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

MG53-induced IRS-1 ubiquitination negatively regulates skeletal myogenesis and insulin signalling.
Nature communications42354(2013)
Yi JS,Park JS,Ham YM,Nguyen N,Lee NR,Hong J,Kim BW,Lee H,Lee CS,Jeong BC,Song HK,Cho H,Kim YK,Lee JS,Park KS,Shin H,Choi I,Lee SH,Park WJ,Park SY,Choi CS,Lin P,Karunasiri M,Tan T,Duann P,Zhu H,Ma J,Ko YG

Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation.
Molecular and cellular biology24(21)9592-600(2004 Nov)
Kim KI,Giannakopoulos NV,Virgin HW,Zhang DE

Human-mouse comparative mapping of the genomic region containing CDK6: localization of an evolutionary breakpoint.
Mammalian genome : official journal of the International Mammalian Genome Society10(7)764-7(1999 Jul)
Thomas JW,Lee-Lin SQ,Green ED

Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis.
Genome research7(11)1085-93(1997 Nov)
Brady KP,Rowe LB,Her H,Stevens TJ,Eppig J,Sussman DJ,Sikela J,Beier DR

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Ube2h cDNA ORF clone, Mus musculus(house mouse)

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