Timp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timp2
Entrez Gene ID	21858
Full Name	tissue inhibitor of metalloproteinase 2
Synonyms	D11Bwg1104e,Timp-2
General protein information	Preferred Names tissue inhibitor of metalloproteinase 2 Names metalloproteinase inhibitor 2 tissue inhibitor of metalloproteinases 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E2\|11 83.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011594.3	NP_035724.2	metalloproteinase inhibitor 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMP2	NP_003246.1
Bos taurus (cattle)	TIMP2	NP_776897.2
Pan troglodytes (chimpanzee)	TIMP2	XP_001146829.2
Macaca mulatta (Rhesus monkey)	TIMP2	XP_001106657.2
Gallus gallus (chicken)	TIMP2	NP_989629.1
Danio rerio (zebrafish)	timp2a	NP_878294.1
Xenopus tropicalis (tropical clawed frog)	timp2	NP_001015760.1
Canis lupus familiaris (dog)	TIMP2	NP_001003082.1
Mus musculus (house mouse)	Timp2	NP_035724.2
Rattus norvegicus (Norway rat)	Timp2	NP_068824.1

Gene Ontology
Bibliography

Related articles in PubMed

Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth.
Saw S, Aiken A, Fang H, McKee TD, Bregant S, Sanchez O, Chen Y, Weiss A, Dickson BC, Czarny B, Sinha A, Fosang A, Dive V, Waterhouse PD, Kislinger T, Khokha R
The Journal of cell biology218(9)3134-3152(2019 Sep)

An in situ hybridization study of MMP-2, -9, -13, -14, TIMP-1, and -2 mRNA in fetal mouse mandibular condylar cartilage as compared with limb bud cartilage.
Takahashi M, Fujikawa K, Angammana R, Shibata S
Gene expression patterns : GEP321-11(2019 06)

The Forkhead box F1 transcription factor inhibits collagen deposition and accumulation of myofibroblasts during liver fibrosis.
Flood HM, Bolte C, Dasgupta N, Sharma A, Zhang Y, Gandhi CR, Kalin TV, Kalinichenko VV
Biology open8(2)(2019 Feb)

Anti-cancer effect of dung beetle glycosaminoglycans on melanoma.
Ahn MY, Kim BJ, Kim HJ, Jin JM, Yoon HJ, Hwang JS, Park KK
BMC cancer19(1)9(2019 Jan)

MicroRNA-410 inhibition of the TIMP2-dependent MAPK pathway confers neuroprotection against oxidative stress-induced apoptosis after ischemic stroke in mice.
Liu NN, Dong ZL, Han LL
Brain research bulletin14345-57(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22687	NM_011594.3 Latest version!	Mus musculus tissue inhibitor of metalloproteinase 2 (Timp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timp2 ( NM_011594.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035724.2 Mus musculus tissue inhibitor of metalloproteinase 2 (Timp2), mRNA.

Price & Availability↑

CloneID	OMu22687
Clone ID Related Accession (Same CDS sequence)	NM_011594.3
Accession Version	NM_011594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Mus musculus(house mouse)
Product	metalloproteinase inhibitor 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK081522.1, AK032818.1 and BQ030835.1. On Mar 25, 2005 this sequence version replaced NM_011594.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK081522.1, AK032818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCCG CGGCCCGCAG CCTCCGGCTG GCGCTCGGCC TCCTGCTGCT AGCCACGCTG 
CTGCGCCCGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACGTAG TGATCAGAGC CAAAGCAGTG AGCGAGAAGG AGGTGGATTC CGGGAATGAC 
ATCTATGGCA ACCCCATCAA GAGGATTCAG TATGAGATCA AGCAGATAAA GATGTTCAAA 
GGACCTGACA AAGACATCGA GTTTATCTAC ACGGCCCCCT CTTCAGCAGT GTGCGGGGTC 
TCGCTGGACG TTGGAGGAAA GAAGGAGTAT CTAATTGCAG GAAAGGCAGA AGGAGATGGC 
AAGATGCACA TTACCCTCTG TGACTTCATT GTGCCCTGGG ACACGCTTAG CATCACCCAG 
AAGAAGAGCC TGAACCACAG GTACCAGATG GGCTGTGAGT GCAAGATCAC TCGCTGTCCC 
ATGATCCCTT GCTACATCTC CTCCCCGGAT GAGTGCCTCT GGATGGACTG GGTCACAGAG 
AAGAGCATCA ATGGGCACCA GGCCAAGTTC TTCGCCTGCA TCAAGAGAAG TGATGGTTCT 
TGCGCGTGGT ACCGCGGGGC GGCACCCCCC AAGCAAGAGT TTCTTGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035724.2
CDS	322..984
Translation	MGAAARSLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPDKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSITQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKSINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Target ORF information:

RefSeq Version	NM_011594.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tissue inhibitor of metalloproteinase 2 (Timp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011594.3

ORF Insert Sequence:

ATGGGCGCCG CGGCCCGCAG CCTCCGGCTG GCGCTCGGCC TCCTGCTGCT AGCCACGCTG 
CTGCGCCCGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACGTAG TGATCAGAGC CAAAGCAGTG AGCGAGAAGG AGGTGGATTC CGGGAATGAC 
ATCTATGGCA ACCCCATCAA GAGGATTCAG TATGAGATCA AGCAGATAAA GATGTTCAAA 
GGACCTGACA AAGACATCGA GTTTATCTAC ACGGCCCCCT CTTCAGCAGT GTGCGGGGTC 
TCGCTGGACG TTGGAGGAAA GAAGGAGTAT CTAATTGCAG GAAAGGCAGA AGGAGATGGC 
AAGATGCACA TTACCCTCTG TGACTTCATT GTGCCCTGGG ACACGCTTAG CATCACCCAG 
AAGAAGAGCC TGAACCACAG GTACCAGATG GGCTGTGAGT GCAAGATCAC TCGCTGTCCC 
ATGATCCCTT GCTACATCTC CTCCCCGGAT GAGTGCCTCT GGATGGACTG GGTCACAGAG 
AAGAGCATCA ATGGGCACCA GGCCAAGTTC TTCGCCTGCA TCAAGAGAAG TGATGGTTCT 
TGCGCGTGGT ACCGCGGGGC GGCACCCCCC AAGCAAGAGT TTCTTGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth.
The Journal of cell biology218(9)3134-3152(2019 Sep)
Saw S,Aiken A,Fang H,McKee TD,Bregant S,Sanchez O,Chen Y,Weiss A,Dickson BC,Czarny B,Sinha A,Fosang A,Dive V,Waterhouse PD,Kislinger T,Khokha R

An in situ hybridization study of MMP-2, -9, -13, -14, TIMP-1, and -2 mRNA in fetal mouse mandibular condylar cartilage as compared with limb bud cartilage.
Gene expression patterns : GEP321-11(2019 06)
Takahashi M,Fujikawa K,Angammana R,Shibata S

The Forkhead box F1 transcription factor inhibits collagen deposition and accumulation of myofibroblasts during liver fibrosis.
Biology open8(2)(2019 Feb)
Flood HM,Bolte C,Dasgupta N,Sharma A,Zhang Y,Gandhi CR,Kalin TV,Kalinichenko VV

Anti-cancer effect of dung beetle glycosaminoglycans on melanoma.
BMC cancer19(1)9(2019 Jan)
Ahn MY,Kim BJ,Kim HJ,Jin JM,Yoon HJ,Hwang JS,Park KK

MicroRNA-410 inhibition of the TIMP2-dependent MAPK pathway confers neuroprotection against oxidative stress-induced apoptosis after ischemic stroke in mice.
Brain research bulletin14345-57(2018 10)
Liu NN,Dong ZL,Han LL