Rufy1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rufy1
Entrez Gene ID	216724
Full Name	RUN and FYVE domain containing 1
Synonyms	3000002E04Rik,Rabip4,ZFYVE12
General protein information	Preferred Names RUN and FYVE domain containing 1 Names RUN and FYVE domain-containing protein 1 FYVE-finger containing protein Rab4a interacting protein rab4-interacting protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314443.1	XP_017169932.1	RUN and FYVE domain-containing protein 1 isoform X3
XM_017314442.2	XP_017169931.1	RUN and FYVE domain-containing protein 1 isoform X2
XM_017314442.1	XP_017169931.1	RUN and FYVE domain-containing protein 1 isoform X2
NM_172557.2	NP_766145.1	RUN and FYVE domain-containing protein 1
NM_172557.3	NP_766145.1	RUN and FYVE domain-containing protein 1
XM_006532841.3	XP_006532904.1	RUN and FYVE domain-containing protein 1 isoform X1
XM_006532841.4	XP_006532904.1	RUN and FYVE domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RUFY1	XP_848829.3
Gallus gallus (chicken)	RUFY1	NP_001244198.1
Homo sapiens (human)	RUFY1	NP_079434.3
Pan troglodytes (chimpanzee)	LOC462328	XP_003950742.1
Rattus norvegicus (Norway rat)	Rufy1	NP_001094197.1
Bos taurus (cattle)	RUFY1	XP_002688531.1
Danio rerio (zebrafish)	LOC100330077	XP_002667361.2
Mus musculus (house mouse)	Rufy1	NP_766145.1
Xenopus tropicalis (tropical clawed frog)	rufy1	XP_004912873.1

Gene Ontology
Bibliography

Related articles in PubMed

Hnrnph1 Is A Quantitative Trait Gene for Methamphetamine Sensitivity.
Yazdani N, Parker CC, Shen Y, Reed ER, Guido MA, Kole LA, Kirkpatrick SL, Lim JE, Sokoloff G, Cheng R, Johnson WE, Palmer AA, Bryant CD
PLoS genetics11(12)e1005713(2015 Dec)

Rufy3, a protein specifically expressed in neurons, interacts with actin-bundling protein Fascin to control the growth of axons.
Wei Z, Sun M, Liu X, Zhang J, Jin Y
Journal of neurochemistry130(5)678-92(2014 Sep)

The Rab4A effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts.
Vukmirica J, Monzo P, Le Marchand-Brustel Y, Cormont M
The Journal of biological chemistry281(47)36360-8(2006 Nov)

The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes.
Mari M, Monzo P, Kaddai V, Keslair F, Gonzalez T, Le Marchand-Brustel Y, Cormont M
Journal of cell science119(Pt 7)1297-306(2006 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rufy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rufy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77841	XM_017314442.2 Latest version!	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu77842	XM_017314443.1 Latest version!	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu03677	NM_172557.3 Latest version!	Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45987	XM_006532841.4 Latest version!	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu77841	XM_017314442.1	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu03677	NM_172557.2	Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45987	XM_006532841.3	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rufy1 ( XM_017314442.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169931.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu77841
Clone ID Related Accession (Same CDS sequence)	XM_017314442.2 , XM_017314442.1
Accession Version	XM_017314442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGGA AGAGAGAGAG ACTGAAAGGA CAGGAAGAAG GAGGGCTGAG 
GCTGAGGAAG AAGGCTTGCT GCCCGCTGCC CGGCAATCTC CCTGCTGGAT ACATCTTGTC 
TCCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169931.1
CDS	170..1318
Translation	MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSASRPRVADSWSAPILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEEEERETERTGRRRAEAEEEGLLPAARQSPCWIHLVSC

Target ORF information:

RefSeq Version	XM_017314442.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314442.2

ORF Insert Sequence:

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGGA AGAGAGAGAG ACTGAAAGGA CAGGAAGAAG GAGGGCTGAG 
GCTGAGGAAG AAGGCTTGCT GCCCGCTGCC CGGCAATCTC CCTGCTGGAT ACATCTTGTC 
TCCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( XM_017314442.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169931.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu77841
Clone ID Related Accession (Same CDS sequence)	XM_017314442.2 , XM_017314442.1
Accession Version	XM_017314442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGGA AGAGAGAGAG ACTGAAAGGA CAGGAAGAAG GAGGGCTGAG 
GCTGAGGAAG AAGGCTTGCT GCCCGCTGCC CGGCAATCTC CCTGCTGGAT ACATCTTGTC 
TCCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169931.1
CDS	501..1649
Translation	MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSASRPRVADSWSAPILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEEEERETERTGRRRAEAEEEGLLPAARQSPCWIHLVSC

Target ORF information:

RefSeq Version	XM_017314442.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314442.1

ORF Insert Sequence:

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGGA AGAGAGAGAG ACTGAAAGGA CAGGAAGAAG GAGGGCTGAG 
GCTGAGGAAG AAGGCTTGCT GCCCGCTGCC CGGCAATCTC CCTGCTGGAT ACATCTTGTC 
TCCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( XM_017314443.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169932.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu77842
Clone ID Related Accession (Same CDS sequence)	XM_017314443.1
Accession Version	XM_017314443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCATG AAGTAACTGA TTTTACAATG TGGGCAAAAA ATTGGAGCGG GTACAGAAAG 
CCAGGATGGG GCGGGTGCAA CAGAGGTCTC ACAGACCAAC AGAGATGTCT CTGGGACTTT 
GAACCACACG TTCCCTATCT AAAGCAAGGA AACAAAACGA AACACAAGGC GCTGAGGCGC 
ATCATAAGCT GTGCAGGAGC TCAGTGTCAG CTCTCAATGT GGGCAGCAAG ATTAACTCTG 
ATGTTAAAAA ACATGTACGA AATCTTTCTT AATCAGCTTT CAGCTGCAAC AGACCGAATT 
TGTTCCCTAC AAAAAGAACA ACAGCAGCTG AGAGAACAGA ACGAAGTAAT TCGAGAAAGA 
AGCGAGAAGA GCGTGGAGAT AACAAAACAG GACACCAAGG TTGAACTGGA GACTTATAAG 
CAGACCCGGC AGGGCCTGGA TGAAATGTAC AGCGATGTGT GGAAACAGCT GAAGGAGGAG 
AAGAAAGTCC GACTGGAACT GGAAAAGGAG CTGGAGTTGC AGATTGGAAT GAAGACGGAG 
ATGGAGATCG CCATGAAGTT GCTGGAGAAG GACACCCATG AGAAGCAAGA CACGCTCGTT 
GCCCTCCGCC AGCAGCTGGA GGAAGTCAAA GCTATTAACT TACAGATGTT TCACAAAGTT 
CAGAGTGCAG AGAGCAGTTT ACAGCAGAAG AATGAAGCCA TCGCATCTTT TGAAGGAAAA 
ACCACTCAGG TGATGTCCAG CATGAAGCAA ATGGAGGAAA GGTTACAGCA GGCGGAACGG 
GCCAGGCAGG CGGCAGAAGA GCGAAGCCAC AAGTTGCAGC AGGAGCTCAG CGGGAGGGGC 
AGTGCCCTGC AGCTCCAGCT GTCCCAGCTG CGGGACCAAT GCTCAGGTCT AGAGAAAGAA 
CTGAAGTCAG AAAAAGAACA AAGACAAGCT CTCCAGAGAG AACTACAGCG TGAGAAAGAC 
ACCTCTTGTC TGCTCCAGAC GGAGCTGCAG CAAGTGGAAG GCCTGAAGAA GGAGCTGCGG 
GAGCTCCAAG ACGAGAAGGC AGAGTTACGG AAGGTCTGTG AGGAGCAGGA GCAAGCCCTC 
CAGGAAATGG GACTACACCT TAGCCAGTCC AAACTGAAGA TGGAAGACAT AAAGGAAGTA 
AATAAGGCAC TGAAGGGCCA CACATGGTTG AAAGATGACG AGGCAACACA TTGTAAGCAG 
TGTGAGAAGG ACTTCTCCAT TTCTCGGAGG AAGCACCACT GCCGAAACTG TGGCCACATT 
TTCTGCAACA CCTGCTCTAG CAACGAGCTG GCCCTGCCCT CCTACCCTAA GCCAGTGCGT 
GTGTGTGACA GCTGCCATAC CCTGCTACTG CAGCGCTGCT CCTCCACTGC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169932.1
CDS	1903..3279
Translation	MKHEVTDFTMWAKNWSGYRKPGWGGCNRGLTDQQRCLWDFEPHVPYLKQGNKTKHKALRRIISCAGAQCQLSMWAARLTLMLKNMYEIFLNQLSAATDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Target ORF information:

RefSeq Version	XM_017314443.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314443.1

ORF Insert Sequence:

ATGAAGCATG AAGTAACTGA TTTTACAATG TGGGCAAAAA ATTGGAGCGG GTACAGAAAG 
CCAGGATGGG GCGGGTGCAA CAGAGGTCTC ACAGACCAAC AGAGATGTCT CTGGGACTTT 
GAACCACACG TTCCCTATCT AAAGCAAGGA AACAAAACGA AACACAAGGC GCTGAGGCGC 
ATCATAAGCT GTGCAGGAGC TCAGTGTCAG CTCTCAATGT GGGCAGCAAG ATTAACTCTG 
ATGTTAAAAA ACATGTACGA AATCTTTCTT AATCAGCTTT CAGCTGCAAC AGACCGAATT 
TGTTCCCTAC AAAAAGAACA ACAGCAGCTG AGAGAACAGA ACGAAGTAAT TCGAGAAAGA 
AGCGAGAAGA GCGTGGAGAT AACAAAACAG GACACCAAGG TTGAACTGGA GACTTATAAG 
CAGACCCGGC AGGGCCTGGA TGAAATGTAC AGCGATGTGT GGAAACAGCT GAAGGAGGAG 
AAGAAAGTCC GACTGGAACT GGAAAAGGAG CTGGAGTTGC AGATTGGAAT GAAGACGGAG 
ATGGAGATCG CCATGAAGTT GCTGGAGAAG GACACCCATG AGAAGCAAGA CACGCTCGTT 
GCCCTCCGCC AGCAGCTGGA GGAAGTCAAA GCTATTAACT TACAGATGTT TCACAAAGTT 
CAGAGTGCAG AGAGCAGTTT ACAGCAGAAG AATGAAGCCA TCGCATCTTT TGAAGGAAAA 
ACCACTCAGG TGATGTCCAG CATGAAGCAA ATGGAGGAAA GGTTACAGCA GGCGGAACGG 
GCCAGGCAGG CGGCAGAAGA GCGAAGCCAC AAGTTGCAGC AGGAGCTCAG CGGGAGGGGC 
AGTGCCCTGC AGCTCCAGCT GTCCCAGCTG CGGGACCAAT GCTCAGGTCT AGAGAAAGAA 
CTGAAGTCAG AAAAAGAACA AAGACAAGCT CTCCAGAGAG AACTACAGCG TGAGAAAGAC 
ACCTCTTGTC TGCTCCAGAC GGAGCTGCAG CAAGTGGAAG GCCTGAAGAA GGAGCTGCGG 
GAGCTCCAAG ACGAGAAGGC AGAGTTACGG AAGGTCTGTG AGGAGCAGGA GCAAGCCCTC 
CAGGAAATGG GACTACACCT TAGCCAGTCC AAACTGAAGA TGGAAGACAT AAAGGAAGTA 
AATAAGGCAC TGAAGGGCCA CACATGGTTG AAAGATGACG AGGCAACACA TTGTAAGCAG 
TGTGAGAAGG ACTTCTCCAT TTCTCGGAGG AAGCACCACT GCCGAAACTG TGGCCACATT 
TTCTGCAACA CCTGCTCTAG CAACGAGCTG GCCCTGCCCT CCTACCCTAA GCCAGTGCGT 
GTGTGTGACA GCTGCCATAC CCTGCTACTG CAGCGCTGCT CCTCCACTGC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( NM_172557.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766145.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.

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CloneID	OMu03677
Clone ID Related Accession (Same CDS sequence)	NM_172557.2 , NM_172557.3
Accession Version	NM_172557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK046633.1. On Apr 6, 2007 this sequence version replaced NM_172557.1. ##Evidence-Data-START## Transcript exon combination :: AK046633.1, SRR1660819.37301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGCT TTCAGCTGCA ACAGACCGAA TTTGTTCCCT ACAAAAAGAA 
CAACAGCAGC TGAGAGAACA GAACGAAGTA ATTCGAGAAA GAAGCGAGAA GAGCGTGGAG 
ATAACAAAAC AGGACACCAA GGTTGAACTG GAGACTTATA AGCAGACCCG GCAGGGCCTG 
GATGAAATGT ACAGCGATGT GTGGAAACAG CTGAAGGAGG AGAAGAAAGT CCGACTGGAA 
CTGGAAAAGG AGCTGGAGTT GCAGATTGGA ATGAAGACGG AGATGGAGAT CGCCATGAAG 
TTGCTGGAGA AGGACACCCA TGAGAAGCAA GACACGCTCG TTGCCCTCCG CCAGCAGCTG 
GAGGAAGTCA AAGCTATTAA CTTACAGATG TTTCACAAAG TTCAGAGTGC AGAGAGCAGT 
TTACAGCAGA AGAATGAAGC CATCGCATCT TTTGAAGGAA AAACCACTCA GGTGATGTCC 
AGCATGAAGC AAATGGAGGA AAGGTTACAG CAGGCGGAAC GGGCCAGGCA GGCGGCAGAA 
GAGCGAAGCC ACAAGTTGCA GCAGGAGCTC AGCGGGAGGG GCAGTGCCCT GCAGCTCCAG 
CTGTCCCAGC TGCGGGACCA ATGCTCAGGT CTAGAGAAAG AACTGAAGTC AGAAAAAGAA 
CAAAGACAAG CTCTCCAGAG AGAACTACAG CGTGAGAAAG ACACCTCTTG TCTGCTCCAG 
ACGGAGCTGC AGCAAGTGGA AGGCCTGAAG AAGGAGCTGC GGGAGCTCCA AGACGAGAAG 
GCAGAGTTAC GGAAGGTCTG TGAGGAGCAG GAGCAAGCCC TCCAGGAAAT GGGACTACAC 
CTTAGCCAGT CCAAACTGAA GATGGAAGAC ATAAAGGAAG TAAATAAGGC ACTGAAGGGC 
CACACATGGT TGAAAGATGA CGAGGCAACA CATTGTAAGC AGTGTGAGAA GGACTTCTCC 
ATTTCTCGGA GGAAGCACCA CTGCCGAAAC TGTGGCCACA TTTTCTGCAA CACCTGCTCT 
AGCAACGAGC TGGCCCTGCC CTCCTACCCT AAGCCAGTGC GTGTGTGTGA CAGCTGCCAT 
ACCCTGCTAC TGCAGCGCTG CTCCTCCACT GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766145.1
CDS	7..2145
Translation	MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSASRPRVADSWSAPILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Target ORF information:

RefSeq Version	NM_172557.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172557.2

ORF Insert Sequence:

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGCT TTCAGCTGCA ACAGACCGAA TTTGTTCCCT ACAAAAAGAA 
CAACAGCAGC TGAGAGAACA GAACGAAGTA ATTCGAGAAA GAAGCGAGAA GAGCGTGGAG 
ATAACAAAAC AGGACACCAA GGTTGAACTG GAGACTTATA AGCAGACCCG GCAGGGCCTG 
GATGAAATGT ACAGCGATGT GTGGAAACAG CTGAAGGAGG AGAAGAAAGT CCGACTGGAA 
CTGGAAAAGG AGCTGGAGTT GCAGATTGGA ATGAAGACGG AGATGGAGAT CGCCATGAAG 
TTGCTGGAGA AGGACACCCA TGAGAAGCAA GACACGCTCG TTGCCCTCCG CCAGCAGCTG 
GAGGAAGTCA AAGCTATTAA CTTACAGATG TTTCACAAAG TTCAGAGTGC AGAGAGCAGT 
TTACAGCAGA AGAATGAAGC CATCGCATCT TTTGAAGGAA AAACCACTCA GGTGATGTCC 
AGCATGAAGC AAATGGAGGA AAGGTTACAG CAGGCGGAAC GGGCCAGGCA GGCGGCAGAA 
GAGCGAAGCC ACAAGTTGCA GCAGGAGCTC AGCGGGAGGG GCAGTGCCCT GCAGCTCCAG 
CTGTCCCAGC TGCGGGACCA ATGCTCAGGT CTAGAGAAAG AACTGAAGTC AGAAAAAGAA 
CAAAGACAAG CTCTCCAGAG AGAACTACAG CGTGAGAAAG ACACCTCTTG TCTGCTCCAG 
ACGGAGCTGC AGCAAGTGGA AGGCCTGAAG AAGGAGCTGC GGGAGCTCCA AGACGAGAAG 
GCAGAGTTAC GGAAGGTCTG TGAGGAGCAG GAGCAAGCCC TCCAGGAAAT GGGACTACAC 
CTTAGCCAGT CCAAACTGAA GATGGAAGAC ATAAAGGAAG TAAATAAGGC ACTGAAGGGC 
CACACATGGT TGAAAGATGA CGAGGCAACA CATTGTAAGC AGTGTGAGAA GGACTTCTCC 
ATTTCTCGGA GGAAGCACCA CTGCCGAAAC TGTGGCCACA TTTTCTGCAA CACCTGCTCT 
AGCAACGAGC TGGCCCTGCC CTCCTACCCT AAGCCAGTGC GTGTGTGTGA CAGCTGCCAT 
ACCCTGCTAC TGCAGCGCTG CTCCTCCACT GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( NM_172557.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766145.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.

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CloneID	OMu03677
Clone ID Related Accession (Same CDS sequence)	NM_172557.2 , NM_172557.3
Accession Version	NM_172557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551196800000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK046625.1, AK046633.1 and BQ176179.1. On Jul 6, 2018 this sequence version replaced NM_172557.2. ##Evidence-Data-START## Transcript exon combination :: AK046633.1, SRR1660819.37301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGCT TTCAGCTGCA ACAGACCGAA TTTGTTCCCT ACAAAAAGAA 
CAACAGCAGC TGAGAGAACA GAACGAAGTA ATTCGAGAAA GAAGCGAGAA GAGCGTGGAG 
ATAACAAAAC AGGACACCAA GGTTGAACTG GAGACTTATA AGCAGACCCG GCAGGGCCTG 
GATGAAATGT ACAGCGATGT GTGGAAACAG CTGAAGGAGG AGAAGAAAGT CCGACTGGAA 
CTGGAAAAGG AGCTGGAGTT GCAGATTGGA ATGAAGACGG AGATGGAGAT CGCCATGAAG 
TTGCTGGAGA AGGACACCCA TGAGAAGCAA GACACGCTCG TTGCCCTCCG CCAGCAGCTG 
GAGGAAGTCA AAGCTATTAA CTTACAGATG TTTCACAAAG TTCAGAGTGC AGAGAGCAGT 
TTACAGCAGA AGAATGAAGC CATCGCATCT TTTGAAGGAA AAACCACTCA GGTGATGTCC 
AGCATGAAGC AAATGGAGGA AAGGTTACAG CAGGCGGAAC GGGCCAGGCA GGCGGCAGAA 
GAGCGAAGCC ACAAGTTGCA GCAGGAGCTC AGCGGGAGGG GCAGTGCCCT GCAGCTCCAG 
CTGTCCCAGC TGCGGGACCA ATGCTCAGGT CTAGAGAAAG AACTGAAGTC AGAAAAAGAA 
CAAAGACAAG CTCTCCAGAG AGAACTACAG CGTGAGAAAG ACACCTCTTG TCTGCTCCAG 
ACGGAGCTGC AGCAAGTGGA AGGCCTGAAG AAGGAGCTGC GGGAGCTCCA AGACGAGAAG 
GCAGAGTTAC GGAAGGTCTG TGAGGAGCAG GAGCAAGCCC TCCAGGAAAT GGGACTACAC 
CTTAGCCAGT CCAAACTGAA GATGGAAGAC ATAAAGGAAG TAAATAAGGC ACTGAAGGGC 
CACACATGGT TGAAAGATGA CGAGGCAACA CATTGTAAGC AGTGTGAGAA GGACTTCTCC 
ATTTCTCGGA GGAAGCACCA CTGCCGAAAC TGTGGCCACA TTTTCTGCAA CACCTGCTCT 
AGCAACGAGC TGGCCCTGCC CTCCTACCCT AAGCCAGTGC GTGTGTGTGA CAGCTGCCAT 
ACCCTGCTAC TGCAGCGCTG CTCCTCCACT GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766145.1
CDS	21..2159
Translation	MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSASRPRVADSWSAPILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Target ORF information:

RefSeq Version	NM_172557.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172557.3

ORF Insert Sequence:

ATGGCCGCCC GCGGAGAATG CCGCCGGGCG GGGCAGGATT CGGAGCCGGA GCCGGAGCGG 
GAGCCGGAGT CCGAGCCGGG GCCGGAGCCT GAGCCCCAAG CAGGCCTAGA GTCCGGGGAA 
GCGTTCGAGA TCGTGGACCG GAGCCAGCTG CCGAGTCCCG GCGAGCTGCG GAGTGCGTCG 
CGGCCTCGAG TGGCCGACAG CTGGTCGGCG CCCATCCTGA CGCTGGCGCG CAGAGCCACC 
GGGAACCTGT CGGCGAGCTG CGGCAGCGCG CTGCGCGCGG CCGCGGGGCT GGGAGACGGC 
GGCGGCGGCG GGGAGCGCGC AGCTTCCAAA GGCCAGATGA TGGAGGAGCG TGCCAACCTA 
ATGCACATGA TGAAACTCAG CATTAAGGTC TTACTCCAGT CTGCCCTTAG CCTGGGACGC 
AGTCTGGATG CAGACTATGC CCCCTTGCAG CAGTTCTTTG TAGTGATGGA GCATTGCCTC 
AAACATGGGC TGAAAGTCAA GAAGAGTTTC ATTGGCCAAA ATAAGTCTTT CTTTGGTCCT 
TTGGAGCTGG TGGAAAAACT TTGTCCTGAA GCATCAGATA TAGCTACCAG TGTCAGGAAT 
CTTCCAGAAC TAAAGACAGC CGTGGGAAGA GGCAGAGCGT GGCTTTATCT TGCTCTCATG 
CAAAAGAAAC TGGCAGATTA TCTGAAAGTC CTCATAGACA ACAAGCAGCT CCTAAGTGAG 
TTCTATGAGC CCGAGGCCTT AATGATGGAG GAGGAAGGAA TGGTGATCGT GGGTCTGCTG 
GTGGGACTCA ACGTTCTGGA TGCCAACCTC TGCTTAAAGG GAGAAGACTT GGATTCTCAG 
GTCGGGGTGA TTGACTTCTC TCTCTGTCTG AAGGACGCAC AGGATCTTGA CAGCGGCAGA 
GAGCATGAAA GAATTACCGA TGTCCTTGAT CAGAAAAATT ACGTGGAGGA ACTTAACCGG 
CACCTAAGCT GCACAGTTGG GGATCTTCAG ACGAAGATAG ATGGCTTGGA AAAGACGAAC 
TCAAAGCTTC AAGAAGAGCT TTCAGCTGCA ACAGACCGAA TTTGTTCCCT ACAAAAAGAA 
CAACAGCAGC TGAGAGAACA GAACGAAGTA ATTCGAGAAA GAAGCGAGAA GAGCGTGGAG 
ATAACAAAAC AGGACACCAA GGTTGAACTG GAGACTTATA AGCAGACCCG GCAGGGCCTG 
GATGAAATGT ACAGCGATGT GTGGAAACAG CTGAAGGAGG AGAAGAAAGT CCGACTGGAA 
CTGGAAAAGG AGCTGGAGTT GCAGATTGGA ATGAAGACGG AGATGGAGAT CGCCATGAAG 
TTGCTGGAGA AGGACACCCA TGAGAAGCAA GACACGCTCG TTGCCCTCCG CCAGCAGCTG 
GAGGAAGTCA AAGCTATTAA CTTACAGATG TTTCACAAAG TTCAGAGTGC AGAGAGCAGT 
TTACAGCAGA AGAATGAAGC CATCGCATCT TTTGAAGGAA AAACCACTCA GGTGATGTCC 
AGCATGAAGC AAATGGAGGA AAGGTTACAG CAGGCGGAAC GGGCCAGGCA GGCGGCAGAA 
GAGCGAAGCC ACAAGTTGCA GCAGGAGCTC AGCGGGAGGG GCAGTGCCCT GCAGCTCCAG 
CTGTCCCAGC TGCGGGACCA ATGCTCAGGT CTAGAGAAAG AACTGAAGTC AGAAAAAGAA 
CAAAGACAAG CTCTCCAGAG AGAACTACAG CGTGAGAAAG ACACCTCTTG TCTGCTCCAG 
ACGGAGCTGC AGCAAGTGGA AGGCCTGAAG AAGGAGCTGC GGGAGCTCCA AGACGAGAAG 
GCAGAGTTAC GGAAGGTCTG TGAGGAGCAG GAGCAAGCCC TCCAGGAAAT GGGACTACAC 
CTTAGCCAGT CCAAACTGAA GATGGAAGAC ATAAAGGAAG TAAATAAGGC ACTGAAGGGC 
CACACATGGT TGAAAGATGA CGAGGCAACA CATTGTAAGC AGTGTGAGAA GGACTTCTCC 
ATTTCTCGGA GGAAGCACCA CTGCCGAAAC TGTGGCCACA TTTTCTGCAA CACCTGCTCT 
AGCAACGAGC TGGCCCTGCC CTCCTACCCT AAGCCAGTGC GTGTGTGTGA CAGCTGCCAT 
ACCCTGCTAC TGCAGCGCTG CTCCTCCACT GCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( XM_006532841.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532904.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.

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CloneID	OMu45987
Clone ID Related Accession (Same CDS sequence)	XM_006532841.3 , XM_006532841.4
Accession Version	XM_006532841.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGCG ACTTGTCTAC GAACCCCGGA TATCCTCCCG CTACTTCCAA AGGCCAGATG 
ATGGAGGAGC GTGCCAACCT AATGCACATG ATGAAACTCA GCATTAAGGT CTTACTCCAG 
TCTGCCCTTA GCCTGGGACG CAGTCTGGAT GCAGACTATG CCCCCTTGCA GCAGTTCTTT 
GTAGTGATGG AGCATTGCCT CAAACATGGG CTGAAAGTCA AGAAGAGTTT CATTGGCCAA 
AATAAGTCTT TCTTTGGTCC TTTGGAGCTG GTGGAAAAAC TTTGTCCTGA AGCATCAGAT 
ATAGCTACCA GTGTCAGGAA TCTTCCAGAA CTAAAGACAG CCGTGGGAAG AGGCAGAGCG 
TGGCTTTATC TTGCTCTCAT GCAAAAGAAA CTGGCAGATT ATCTGAAAGT CCTCATAGAC 
AACAAGCAGC TCCTAAGTGA GTTCTATGAG CCCGAGGCCT TAATGATGGA GGAGGAAGGA 
ATGGTGATCG TGGGTCTGCT GGTGGGACTC AACGTTCTGG ATGCCAACCT CTGCTTAAAG 
GGAGAAGACT TGGATTCTCA GGTCGGGGTG ATTGACTTCT CTCTCTGTCT GAAGGACGCA 
CAGGATCTTG ACAGCGGCAG AGAGCATGAA AGAATTACCG ATGTCCTTGA TCAGAAAAAT 
TACGTGGAGG AACTTAACCG GCACCTAAGC TGCACAGTTG GGGATCTTCA GACGAAGATA 
GATGGCTTGG AAAAGACGAA CTCAAAGCTT CAAGAAGAGC TTTCAGCTGC AACAGACCGA 
ATTTGTTCCC TACAAAAAGA ACAACAGCAG CTGAGAGAAC AGAACGAAGT AATTCGAGAA 
AGAAGCGAGA AGAGCGTGGA GATAACAAAA CAGGACACCA AGGTTGAACT GGAGACTTAT 
AAGCAGACCC GGCAGGGCCT GGATGAAATG TACAGCGATG TGTGGAAACA GCTGAAGGAG 
GAGAAGAAAG TCCGACTGGA ACTGGAAAAG GAGCTGGAGT TGCAGATTGG AATGAAGACG 
GAGATGGAGA TCGCCATGAA GTTGCTGGAG AAGGACACCC ATGAGAAGCA AGACACGCTC 
GTTGCCCTCC GCCAGCAGCT GGAGGAAGTC AAAGCTATTA ACTTACAGAT GTTTCACAAA 
GTTCAGAGTG CAGAGAGCAG TTTACAGCAG AAGAATGAAG CCATCGCATC TTTTGAAGGA 
AAAACCACTC AGGTGATGTC CAGCATGAAG CAAATGGAGG AAAGGTTACA GCAGGCGGAA 
CGGGCCAGGC AGGCGGCAGA AGAGCGAAGC CACAAGTTGC AGCAGGAGCT CAGCGGGAGG 
GGCAGTGCCC TGCAGCTCCA GCTGTCCCAG CTGCGGGACC AATGCTCAGG TCTAGAGAAA 
GAACTGAAGT CAGAAAAAGA ACAAAGACAA GCTCTCCAGA GAGAACTACA GCGTGAGAAA 
GACACCTCTT GTCTGCTCCA GACGGAGCTG CAGCAAGTGG AAGGCCTGAA GAAGGAGCTG 
CGGGAGCTCC AAGACGAGAA GGCAGAGTTA CGGAAGGTCT GTGAGGAGCA GGAGCAAGCC 
CTCCAGGAAA TGGGACTACA CCTTAGCCAG TCCAAACTGA AGATGGAAGA CATAAAGGAA 
GTAAATAAGG CACTGAAGGG CCACACATGG TTGAAAGATG ACGAGGCAAC ACATTGTAAG 
CAGTGTGAGA AGGACTTCTC CATTTCTCGG AGGAAGCACC ACTGCCGAAA CTGTGGCCAC 
ATTTTCTGCA ACACCTGCTC TAGCAACGAG CTGGCCCTGC CCTCCTACCC TAAGCCAGTG 
CGTGTGTGTG ACAGCTGCCA TACCCTGCTA CTGCAGCGCT GCTCCTCCAC TGCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532904.1
CDS	110..1969
Translation	MECDLSTNPGYPPATSKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Target ORF information:

RefSeq Version	XM_006532841.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532841.3

ORF Insert Sequence:

ATGGAATGCG ACTTGTCTAC GAACCCCGGA TATCCTCCCG CTACTTCCAA AGGCCAGATG 
ATGGAGGAGC GTGCCAACCT AATGCACATG ATGAAACTCA GCATTAAGGT CTTACTCCAG 
TCTGCCCTTA GCCTGGGACG CAGTCTGGAT GCAGACTATG CCCCCTTGCA GCAGTTCTTT 
GTAGTGATGG AGCATTGCCT CAAACATGGG CTGAAAGTCA AGAAGAGTTT CATTGGCCAA 
AATAAGTCTT TCTTTGGTCC TTTGGAGCTG GTGGAAAAAC TTTGTCCTGA AGCATCAGAT 
ATAGCTACCA GTGTCAGGAA TCTTCCAGAA CTAAAGACAG CCGTGGGAAG AGGCAGAGCG 
TGGCTTTATC TTGCTCTCAT GCAAAAGAAA CTGGCAGATT ATCTGAAAGT CCTCATAGAC 
AACAAGCAGC TCCTAAGTGA GTTCTATGAG CCCGAGGCCT TAATGATGGA GGAGGAAGGA 
ATGGTGATCG TGGGTCTGCT GGTGGGACTC AACGTTCTGG ATGCCAACCT CTGCTTAAAG 
GGAGAAGACT TGGATTCTCA GGTCGGGGTG ATTGACTTCT CTCTCTGTCT GAAGGACGCA 
CAGGATCTTG ACAGCGGCAG AGAGCATGAA AGAATTACCG ATGTCCTTGA TCAGAAAAAT 
TACGTGGAGG AACTTAACCG GCACCTAAGC TGCACAGTTG GGGATCTTCA GACGAAGATA 
GATGGCTTGG AAAAGACGAA CTCAAAGCTT CAAGAAGAGC TTTCAGCTGC AACAGACCGA 
ATTTGTTCCC TACAAAAAGA ACAACAGCAG CTGAGAGAAC AGAACGAAGT AATTCGAGAA 
AGAAGCGAGA AGAGCGTGGA GATAACAAAA CAGGACACCA AGGTTGAACT GGAGACTTAT 
AAGCAGACCC GGCAGGGCCT GGATGAAATG TACAGCGATG TGTGGAAACA GCTGAAGGAG 
GAGAAGAAAG TCCGACTGGA ACTGGAAAAG GAGCTGGAGT TGCAGATTGG AATGAAGACG 
GAGATGGAGA TCGCCATGAA GTTGCTGGAG AAGGACACCC ATGAGAAGCA AGACACGCTC 
GTTGCCCTCC GCCAGCAGCT GGAGGAAGTC AAAGCTATTA ACTTACAGAT GTTTCACAAA 
GTTCAGAGTG CAGAGAGCAG TTTACAGCAG AAGAATGAAG CCATCGCATC TTTTGAAGGA 
AAAACCACTC AGGTGATGTC CAGCATGAAG CAAATGGAGG AAAGGTTACA GCAGGCGGAA 
CGGGCCAGGC AGGCGGCAGA AGAGCGAAGC CACAAGTTGC AGCAGGAGCT CAGCGGGAGG 
GGCAGTGCCC TGCAGCTCCA GCTGTCCCAG CTGCGGGACC AATGCTCAGG TCTAGAGAAA 
GAACTGAAGT CAGAAAAAGA ACAAAGACAA GCTCTCCAGA GAGAACTACA GCGTGAGAAA 
GACACCTCTT GTCTGCTCCA GACGGAGCTG CAGCAAGTGG AAGGCCTGAA GAAGGAGCTG 
CGGGAGCTCC AAGACGAGAA GGCAGAGTTA CGGAAGGTCT GTGAGGAGCA GGAGCAAGCC 
CTCCAGGAAA TGGGACTACA CCTTAGCCAG TCCAAACTGA AGATGGAAGA CATAAAGGAA 
GTAAATAAGG CACTGAAGGG CCACACATGG TTGAAAGATG ACGAGGCAAC ACATTGTAAG 
CAGTGTGAGA AGGACTTCTC CATTTCTCGG AGGAAGCACC ACTGCCGAAA CTGTGGCCAC 
ATTTTCTGCA ACACCTGCTC TAGCAACGAG CTGGCCCTGC CCTCCTACCC TAAGCCAGTG 
CGTGTGTGTG ACAGCTGCCA TACCCTGCTA CTGCAGCGCT GCTCCTCCAC TGCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rufy1 ( XM_006532841.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532904.1 Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.

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CloneID	OMu45987
Clone ID Related Accession (Same CDS sequence)	XM_006532841.3 , XM_006532841.4
Accession Version	XM_006532841.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	RUN and FYVE domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532841.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGCG ACTTGTCTAC GAACCCCGGA TATCCTCCCG CTACTTCCAA AGGCCAGATG 
ATGGAGGAGC GTGCCAACCT AATGCACATG ATGAAACTCA GCATTAAGGT CTTACTCCAG 
TCTGCCCTTA GCCTGGGACG CAGTCTGGAT GCAGACTATG CCCCCTTGCA GCAGTTCTTT 
GTAGTGATGG AGCATTGCCT CAAACATGGG CTGAAAGTCA AGAAGAGTTT CATTGGCCAA 
AATAAGTCTT TCTTTGGTCC TTTGGAGCTG GTGGAAAAAC TTTGTCCTGA AGCATCAGAT 
ATAGCTACCA GTGTCAGGAA TCTTCCAGAA CTAAAGACAG CCGTGGGAAG AGGCAGAGCG 
TGGCTTTATC TTGCTCTCAT GCAAAAGAAA CTGGCAGATT ATCTGAAAGT CCTCATAGAC 
AACAAGCAGC TCCTAAGTGA GTTCTATGAG CCCGAGGCCT TAATGATGGA GGAGGAAGGA 
ATGGTGATCG TGGGTCTGCT GGTGGGACTC AACGTTCTGG ATGCCAACCT CTGCTTAAAG 
GGAGAAGACT TGGATTCTCA GGTCGGGGTG ATTGACTTCT CTCTCTGTCT GAAGGACGCA 
CAGGATCTTG ACAGCGGCAG AGAGCATGAA AGAATTACCG ATGTCCTTGA TCAGAAAAAT 
TACGTGGAGG AACTTAACCG GCACCTAAGC TGCACAGTTG GGGATCTTCA GACGAAGATA 
GATGGCTTGG AAAAGACGAA CTCAAAGCTT CAAGAAGAGC TTTCAGCTGC AACAGACCGA 
ATTTGTTCCC TACAAAAAGA ACAACAGCAG CTGAGAGAAC AGAACGAAGT AATTCGAGAA 
AGAAGCGAGA AGAGCGTGGA GATAACAAAA CAGGACACCA AGGTTGAACT GGAGACTTAT 
AAGCAGACCC GGCAGGGCCT GGATGAAATG TACAGCGATG TGTGGAAACA GCTGAAGGAG 
GAGAAGAAAG TCCGACTGGA ACTGGAAAAG GAGCTGGAGT TGCAGATTGG AATGAAGACG 
GAGATGGAGA TCGCCATGAA GTTGCTGGAG AAGGACACCC ATGAGAAGCA AGACACGCTC 
GTTGCCCTCC GCCAGCAGCT GGAGGAAGTC AAAGCTATTA ACTTACAGAT GTTTCACAAA 
GTTCAGAGTG CAGAGAGCAG TTTACAGCAG AAGAATGAAG CCATCGCATC TTTTGAAGGA 
AAAACCACTC AGGTGATGTC CAGCATGAAG CAAATGGAGG AAAGGTTACA GCAGGCGGAA 
CGGGCCAGGC AGGCGGCAGA AGAGCGAAGC CACAAGTTGC AGCAGGAGCT CAGCGGGAGG 
GGCAGTGCCC TGCAGCTCCA GCTGTCCCAG CTGCGGGACC AATGCTCAGG TCTAGAGAAA 
GAACTGAAGT CAGAAAAAGA ACAAAGACAA GCTCTCCAGA GAGAACTACA GCGTGAGAAA 
GACACCTCTT GTCTGCTCCA GACGGAGCTG CAGCAAGTGG AAGGCCTGAA GAAGGAGCTG 
CGGGAGCTCC AAGACGAGAA GGCAGAGTTA CGGAAGGTCT GTGAGGAGCA GGAGCAAGCC 
CTCCAGGAAA TGGGACTACA CCTTAGCCAG TCCAAACTGA AGATGGAAGA CATAAAGGAA 
GTAAATAAGG CACTGAAGGG CCACACATGG TTGAAAGATG ACGAGGCAAC ACATTGTAAG 
CAGTGTGAGA AGGACTTCTC CATTTCTCGG AGGAAGCACC ACTGCCGAAA CTGTGGCCAC 
ATTTTCTGCA ACACCTGCTC TAGCAACGAG CTGGCCCTGC CCTCCTACCC TAAGCCAGTG 
CGTGTGTGTG ACAGCTGCCA TACCCTGCTA CTGCAGCGCT GCTCCTCCAC TGCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532904.1
CDS	109..1968
Translation	MECDLSTNPGYPPATSKGQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIASFEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKEQRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMEDIKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

Target ORF information:

RefSeq Version	XM_006532841.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RUN and FYVE domain containing 1 (Rufy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532841.4

ORF Insert Sequence:

ATGGAATGCG ACTTGTCTAC GAACCCCGGA TATCCTCCCG CTACTTCCAA AGGCCAGATG 
ATGGAGGAGC GTGCCAACCT AATGCACATG ATGAAACTCA GCATTAAGGT CTTACTCCAG 
TCTGCCCTTA GCCTGGGACG CAGTCTGGAT GCAGACTATG CCCCCTTGCA GCAGTTCTTT 
GTAGTGATGG AGCATTGCCT CAAACATGGG CTGAAAGTCA AGAAGAGTTT CATTGGCCAA 
AATAAGTCTT TCTTTGGTCC TTTGGAGCTG GTGGAAAAAC TTTGTCCTGA AGCATCAGAT 
ATAGCTACCA GTGTCAGGAA TCTTCCAGAA CTAAAGACAG CCGTGGGAAG AGGCAGAGCG 
TGGCTTTATC TTGCTCTCAT GCAAAAGAAA CTGGCAGATT ATCTGAAAGT CCTCATAGAC 
AACAAGCAGC TCCTAAGTGA GTTCTATGAG CCCGAGGCCT TAATGATGGA GGAGGAAGGA 
ATGGTGATCG TGGGTCTGCT GGTGGGACTC AACGTTCTGG ATGCCAACCT CTGCTTAAAG 
GGAGAAGACT TGGATTCTCA GGTCGGGGTG ATTGACTTCT CTCTCTGTCT GAAGGACGCA 
CAGGATCTTG ACAGCGGCAG AGAGCATGAA AGAATTACCG ATGTCCTTGA TCAGAAAAAT 
TACGTGGAGG AACTTAACCG GCACCTAAGC TGCACAGTTG GGGATCTTCA GACGAAGATA 
GATGGCTTGG AAAAGACGAA CTCAAAGCTT CAAGAAGAGC TTTCAGCTGC AACAGACCGA 
ATTTGTTCCC TACAAAAAGA ACAACAGCAG CTGAGAGAAC AGAACGAAGT AATTCGAGAA 
AGAAGCGAGA AGAGCGTGGA GATAACAAAA CAGGACACCA AGGTTGAACT GGAGACTTAT 
AAGCAGACCC GGCAGGGCCT GGATGAAATG TACAGCGATG TGTGGAAACA GCTGAAGGAG 
GAGAAGAAAG TCCGACTGGA ACTGGAAAAG GAGCTGGAGT TGCAGATTGG AATGAAGACG 
GAGATGGAGA TCGCCATGAA GTTGCTGGAG AAGGACACCC ATGAGAAGCA AGACACGCTC 
GTTGCCCTCC GCCAGCAGCT GGAGGAAGTC AAAGCTATTA ACTTACAGAT GTTTCACAAA 
GTTCAGAGTG CAGAGAGCAG TTTACAGCAG AAGAATGAAG CCATCGCATC TTTTGAAGGA 
AAAACCACTC AGGTGATGTC CAGCATGAAG CAAATGGAGG AAAGGTTACA GCAGGCGGAA 
CGGGCCAGGC AGGCGGCAGA AGAGCGAAGC CACAAGTTGC AGCAGGAGCT CAGCGGGAGG 
GGCAGTGCCC TGCAGCTCCA GCTGTCCCAG CTGCGGGACC AATGCTCAGG TCTAGAGAAA 
GAACTGAAGT CAGAAAAAGA ACAAAGACAA GCTCTCCAGA GAGAACTACA GCGTGAGAAA 
GACACCTCTT GTCTGCTCCA GACGGAGCTG CAGCAAGTGG AAGGCCTGAA GAAGGAGCTG 
CGGGAGCTCC AAGACGAGAA GGCAGAGTTA CGGAAGGTCT GTGAGGAGCA GGAGCAAGCC 
CTCCAGGAAA TGGGACTACA CCTTAGCCAG TCCAAACTGA AGATGGAAGA CATAAAGGAA 
GTAAATAAGG CACTGAAGGG CCACACATGG TTGAAAGATG ACGAGGCAAC ACATTGTAAG 
CAGTGTGAGA AGGACTTCTC CATTTCTCGG AGGAAGCACC ACTGCCGAAA CTGTGGCCAC 
ATTTTCTGCA ACACCTGCTC TAGCAACGAG CTGGCCCTGC CCTCCTACCC TAAGCCAGTG 
CGTGTGTGTG ACAGCTGCCA TACCCTGCTA CTGCAGCGCT GCTCCTCCAC TGCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hnrnph1 Is A Quantitative Trait Gene for Methamphetamine Sensitivity.
PLoS genetics11(12)e1005713(2015 Dec)
Yazdani N,Parker CC,Shen Y,Reed ER,Guido MA,Kole LA,Kirkpatrick SL,Lim JE,Sokoloff G,Cheng R,Johnson WE,Palmer AA,Bryant CD

Rufy3, a protein specifically expressed in neurons, interacts with actin-bundling protein Fascin to control the growth of axons.
Journal of neurochemistry130(5)678-92(2014 Sep)
Wei Z,Sun M,Liu X,Zhang J,Jin Y

The Rab4A effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts.
The Journal of biological chemistry281(47)36360-8(2006 Nov)
Vukmirica J,Monzo P,Le Marchand-Brustel Y,Cormont M

The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes.
Journal of cell science119(Pt 7)1297-306(2006 Apr)
Mari M,Monzo P,Kaddai V,Keslair F,Gonzalez T,Le Marchand-Brustel Y,Cormont M

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Rufy1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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