Slit1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slit1
Entrez Gene ID	20562
Full Name	slit guidance ligand 1
Synonyms	Slil1,mKIAA0813
General protein information	Preferred Names slit guidance ligand 1 Names slit homolog 1 protein slit-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247200.2	XP_011245502.1	slit homolog 1 protein isoform X1
NM_015748.3	NP_056563.2	slit homolog 1 protein precursor
XM_030250829.1	XP_030106689.1	slit homolog 1 protein isoform X1
XM_030250830.1	XP_030106690.1	slit homolog 1 protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLIT1	XP_003312806.1
Homo sapiens (human)	SLIT1	NP_003052.2
Bos taurus (cattle)	SLIT1	XP_002698459.1
Xenopus tropicalis (tropical clawed frog)	slit1	NP_001090702.1
Macaca mulatta (Rhesus monkey)	SLIT1	XP_001094231.2
Canis lupus familiaris (dog)	SLIT1	XP_005637640.1
Mus musculus (house mouse)	Slit1	NP_056563.2
Rattus norvegicus (Norway rat)	Slit1	NP_075242.1
Gallus gallus (chicken)	SLIT1	NP_001264265.1
Danio rerio (zebrafish)	slit1a	NP_001030140.1

Gene Ontology
Bibliography

Related articles in PubMed

Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge.
Dun XP, Carr L, Woodley PK, Barry RW, Drake LK, Mindos T, Roberts SL, Lloyd AC, Parkinson DB
Cell reports26(6)1458-1472.e4(2019 02)

A refinement to the formalin test in mice.
Lopes DM, Cater HL, Thakur M, Wells S, McMahon SB
F1000Research8891(2019)

Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors.
Escot S, Willnow D, Naumann H, Di Francescantonio S, Spagnoli FM
Nature communications9(1)5082(2018 11)

ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-β signalling.
Pinho AV, Van Bulck M, Chantrill L, Arshi M, Sklyarova T, Herrmann D, Vennin C, Gallego-Ortega D, Mawson A, Giry-Laterriere M, Magenau A, Leuckx G, Baeyens L, Gill AJ, Phillips P, Timpson P, Biankin AV, Wu J, Rooman I
Nature communications9(1)5083(2018 11)

Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels.
Cárdenas A, Villalba A, de Juan Romero C, Picó E, Kyrousi C, Tzika AC, Tessier-Lavigne M, Ma L, Drukker M, Cappello S, Borrell V
Cell174(3)590-606.e21(2018 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Slit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19364	NM_015748.3 Latest version!	Mus musculus slit guidance ligand 1 (Slit1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19364	XM_011247200.2 Latest version!	Mus musculus slit homolog 1 (Drosophila) (Slit1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19364	XM_030250830.1 Latest version!	Mus musculus slit guidance ligand 1 (Slit1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19364	XM_030250829.1 Latest version!	Mus musculus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slit1 ( NM_015748.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056563.2 Mus musculus slit guidance ligand 1 (Slit1), mRNA.

Price & Availability↑

CloneID	OMu19364
Clone ID Related Accession (Same CDS sequence)	XM_011247200.2 , NM_015748.3 , XM_030250829.1 , XM_030250830.1
Accession Version	NM_015748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Mus musculus(house mouse)
Product	slit homolog 1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC145557.3 and BC062091.1. On Aug 21, 2009 this sequence version replaced NM_015748.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057131.1, AF144627.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056563.2
CDS	449..5044
Translation	MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPDMNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEIPPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA

Target ORF information:

RefSeq Version	NM_015748.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 1 (Slit1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015748.3

ORF Insert Sequence:

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit1 ( XM_011247200.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245502.1 Mus musculus slit homolog 1 (Drosophila) (Slit1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19364
Clone ID Related Accession (Same CDS sequence)	XM_011247200.2 , NM_015748.3 , XM_030250829.1 , XM_030250830.1
Accession Version	XM_011247200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	slit homolog 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245502.1
CDS	327..4922
Translation	MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPDMNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEIPPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA

Target ORF information:

RefSeq Version	XM_011247200.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit homolog 1 (Drosophila) (Slit1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247200.2

ORF Insert Sequence:

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit1 ( XM_030250829.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106689.1 Mus musculus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.

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CloneID	OMu19364
Clone ID Related Accession (Same CDS sequence)	XM_011247200.2 , NM_015748.3 , XM_030250829.1 , XM_030250830.1
Accession Version	XM_030250829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	slit homolog 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106689.1
CDS	311..4906
Translation	MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPDMNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEIPPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA

Target ORF information:

RefSeq Version	XM_030250829.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 1 (Slit1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250829.1

ORF Insert Sequence:

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit1 ( XM_030250830.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106690.1 Mus musculus slit guidance ligand 1 (Slit1), transcript variant X2, mRNA.

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CloneID	OMu19364
Clone ID Related Accession (Same CDS sequence)	XM_011247200.2 , NM_015748.3 , XM_030250829.1 , XM_030250830.1
Accession Version	XM_030250830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	slit homolog 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106690.1
CDS	418..5013
Translation	MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPDMNPCQHEAQCVGTPDGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEIPPAPRSSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQCQDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATKGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRGYAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAQCA

Target ORF information:

RefSeq Version	XM_030250830.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 1 (Slit1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250830.1

ORF Insert Sequence:

ATGGCGCTAA CGCCCCAGCG CGGGTCCTCT TCGGGGTTGA GCCGACCGGA GCTGTGGCTG 
CTGCTGTGGG CAGCCGCGTG GCGCCTGGGG GCCACGGCGT GCCCCGCCCT CTGCACCTGC 
ACCGGCACTA CGGTGGACTG CCACGGCACG GGTTTGCAGG CCATCCCCAA GAACATCCCA 
CGGAACACCG AGCGCCTGGA ACTCAACGGC AACAACATCA CTCGGATCCA CAAGAATGAC 
TTTGCTGGCC TCAAGCAGCT TCGAGTGTTG CAGCTGATGG AGAACCAGAT CGGAGCTGTG 
GAACGCGGAG CTTTTGATGA CATGAAGGAG CTAGAGCGCC TACGGCTGAA CCGCAACCAG 
CTACAAGTGT TGCCTGAACT ACTGTTCCAG AACAACCAGG CTCTATCCAG ACTGGACCTG 
AGTGAGAATT TTCTCCAGGC TGTGCCCAGG AAGGCTTTTC GGGGAGCCAC AGACCTCAAA 
AATCTACAGC TGGACAAGAA CCGGATCAGC TGCATCGAGG AAGGGGCCTT CCGTGCCCTA 
CGGGGACTGG AGGTTCTGAC CCTGAACAAC AACAACATCA CCACCATCCC TGTGTCCAGT 
TTCAACCATA TGCCCAAGCT TCGGACCTTC CGGCTGCACT CCAACCACCT GTTCTGTGAC 
TGCCACCTGG CCTGGCTCTC TCAGTGGCTG AGACAGAGGC CCACCATCGG ACTCTTCACC 
CAGTGCTCTG GGCCCGCCAG CCTTCGGGGC CTCAACGTGG CAGAGGTGCA AAAGGGCGAG 
TTCAGCTGCT CAGGCCAGGG GGAGGCTGCG GGAGCACCTG CCTGCACCCT CTCGTCCGGT 
TCCTGCCCAG CCATGTGCAG CTGTAGCAGC GGCATCGTGG ACTGCCGTGG CAAGGGCCTC 
ACTGCCATCC CTGCTAACCT TCCAGAGACC ATGACAGAGA TCCGCCTGGA GTTGAACGGT 
ATCAAATCCA TCCCACCCGG AGCCTTCTCT CCCTACAGAA AGCTGCGCAG GATAGACCTG 
AGCAACAACC AGATTGCAGA GATTGCACCC GACGCCTTCC AAGGCCTGCG CTCCTTGAAT 
TCCCTGGTCC TCTATGGCAA CAAGATTACA GACCTGCCCC GAGGTGTATT TGGAGGCCTG 
TACACACTCC AGCTGCTGCT TCTAAATGCC AACAAGATCA ACTGCATCCG GCCTGATGCC 
TTCCAGGACC TGCAGAACCT CTCTCTGCTC TCCCTGTATG ACAACAAGAT CCAGAGTCTC 
GCCAAGGGCA CCTTCACCTC CCTACGGGCC ATCCAGACAC TGCATCTGGC CCAGAATCCT 
TTCATCTGTG ACTGTAACCT CAAGTGGCTG GCAGACTTCT TACGTACCAA CCCCATCGAG 
ACAACTGGGG CCCGATGTGC CAGCCCCCGG CGCCTCGCCA ACAAGCGTAT TGGGCAGATC 
AAAAGCAAGA AGTTTCGGTG CTCAGCCAAA GAGCAGTACT TCATTCCAGG GACAGAGGAC 
TACCACCTGA ACAGCGAGTG TACCAGCGAC GTGGCCTGCC CACACAAGTG CCGTTGTGAG 
GCCAGTGTGG TGGAGTGCTC CAGCCTGAAA CTCTCTAAGA TCCCTGAGCG AATCCCCCAG 
TCCACGACTG AGCTACGACT GAACAACAAT GAGATTTCTA TCCTAGAGGC CACTGGGTTG 
TTTAAGAAGC TCTCACATCT GAAGAAAATA AACCTGAGCA ATAACAAGGT GTCCGAGATC 
GAAGACGGGA CGTTTGAGGG TGCCGCCTCT GTCAGTGAGC TGCACCTGAC TGCTAACCAG 
CTGGAGTCCA TTAGAAGTGG CATGTTCCGG GGTCTAGATG GCTTGCGGAC CCTGATGCTG 
AGGAACAACC GTATCAGCTG TATCCACAAC GACAGCTTCA CCGGGCTGCG CAACGTGCGG 
CTCCTGTCTC TCTATGACAA CCACATCACC ACCATCTCCC CTGGAGCCTT TGACACTCTC 
CAGGCCCTGT CCACACTAAA CCTCCTGGCC AACCCGTTCA ACTGCAACTG CCATCTGTCA 
TGGCTGGGCG ACTGGCTCCG GAAGAGGAAG ATTGTGACGG GGAACCCTCG CTGTCAGAAC 
CCAGACTTCC TGCGGCAGAT TCCTCTGCAG GATGTGGCCT TCCCTGACTT CAGGTGTGAG 
GAAGGCCAGG AGGAGGTGGG CTGCCTGCCC CGCCCACAGT GCCCCCAGGA GTGTGCCTGC 
CTGGACACAG TGGTCCGCTG CAGCAACAAG CACCTCCAGG CTCTGCCCAA GGGCATCCCC 
AAGAATGTCA CAGAACTCTA TTTGGATGGG AACCAGTTCA CACTCGTTCC GGGACAGCTG 
TCTACCTTCA AGTATCTGCA GCTCGTGGAC TTGAGCAACA ACAAGATCAG TTCCTTGAGC 
AATTCTTCCT TCACCAACAT GAGCCAGCTG ACCACGCTGA TCCTCAGCTA CAATGCCCTC 
CAGTGCATCC CTCCTCTGGC CTTTCAAGGA CTTCGTTCGC TGCGCCTGCT GTCTTTGCAT 
GGCAATGATG TCTCCACCCT CCAAGAGGGC ATCTTTGCAG ACGTGACGTC TCTGTCTCAC 
CTGGCCATTG GTGCCAACCC CCTGTACTGC GACTGCCGCC TCCGGTGGCT CTCCAGTTGG 
GTGAAGACTG GCTACAAGGA ACCTGGCATT GCCCGCTGTG CTGGACCCCC AGAGATGGAG 
GGCAAACTGT TACTCACCAC ACCTGCCAAG AAGTTTGAAT GCCAAGGTCC CCCCAGCCTG 
GCAGTTCAGG CCAAGTGTGA TCCCTGCTTG TCCAGCCCAT GCCAGAACCA GGGCACCTGC 
CACAATGACC CTCTTGAGGT ATACAGGTGT ACCTGCCCCA GTGGCTACAA GGGCCGACAC 
TGTGAGGTGT CTCTGGACGG CTGTTCCAGC AACCCATGTG GAAATGGGGG GACCTGCCAT 
GCCCAGGAGG GCGAGGATGC TGGCTTCACG TGCTCCTGTC CCTCCGGCTT TGAAGGGCCG 
ACCTGTGGAG TGGACACAGA TGACTGTGTT AAGCATGCCT GTGTCAACGG GGGTGTCTGT 
GTAGATGGCG TGGGGAATTA CACATGTCAG TGCCCCCTGC AGTACACAGG AAGGGCCTGT 
GAGCAGCTGG TGGACTTCTG CTCCCCGGAT ATGAACCCGT GTCAGCATGA GGCCCAATGT 
GTTGGCACCC CGGATGGGCC CAGGTGTGAG TGCATGCTAG GTTACACGGG TGACAACTGC 
AGCGAAAATC AGGATGACTG CAAGGACCAC AAGTGTCAGA ACGGGGCCCA GTGTGTGGAT 
GAAGTCAACA GCTACGCCTG CCTCTGTGTT GAGGGCTACA GTGGACAGCT CTGTGAGATC 
CCACCTGCCC CCAGGAGCTC CTGTGAGGGG ACGGAGTGCC AGAATGGAGC CAACTGCGTA 
GACCAGGGAA GCCGGCCCGT ATGCCAGTGC CTACCAGGCT TTGGTGGCCC TGAATGTGAG 
AAATTGCTCA GTGTCAACTT TGTGGACCGG GACACTTATC TGCAGTTCAC TGACCTGCAG 
AACTGGCCTC GGGCCAACAT CACTCTTCAG GTCTCCACAG CAGAGGACAA TGGGATTCTC 
CTATATAATG GGGACAATGA CCACATTGCA GTTGAGCTGT ACCAGGGCCA TGTCCGTGTT 
AGTTATGACC CAGGCAGCTA CCCCAGCTCT GCTATCTACA GCGCTGAGAC GATCAACGAT 
GGGCAGTTCC ACACCGTTGA GCTGGTCACC TTCGACCAGA TGGTGAACCT CTCCATCGAT 
GGCGGCAGCC CCATGACCAT GGACAACTTT GGCAAGCACT ACACCCTCAA CAGCGAGGCC 
CCCCTCTATG TGGGAGGGAT GCCTGTGGAC GTGAACTCAG CTGCCTTCCG CCTGTGGCAG 
ATCCTCAATG GTACCAGCTT CCACGGTTGC ATCCGGAACC TGTACATCAA CAATGAACTG 
CAGGACTTCA CCAAGACACA GATGAAGCCA GGCGTGGTGC CAGGCTGTGA GCCGTGCCGC 
AAACTCTACT GTCTACATGG CATTTGCCAG CCCAACGCCA CCCCAGGGCC TGTGTGCCAC 
TGTGAGGCTG GCTGGGGGGG CCTGCACTGT GACCAGCCAG TGGATGGCCC CTGCCATGGC 
CACAAGTGTG TCCATGGGAA ATGCGTGCCG CTCGACGCTC TTGCCTACAG CTGCCAGTGC 
CAGGATGGGT ACTCGGGGGC TCTGTGCAAC CAGGTCGGGG CTGTGGCAGA GCCCTGTGGG 
GGCTTGCAGT GCCTGCATGG TCACTGCCAG GCCTCTGCCA CCAAAGGGGC ACACTGTGTG 
TGCAGCCCAG GCTTTTCAGG CGAGCTGTGT GAGCAAGAGT CCGAGTGCCG GGGGGACCCT 
GTCCGGGACT TTCACCGGGT CCAGAGGGGC TATGCCATCT GCCAGACCAC ACGCCCGCTG 
TCGTGGGTGG AATGCCGGGG CGCGTGCCCG GGCCAGGGCT GCTGCCAGGG CCTGCGGCTG 
AAGCGGAGGA AGCTCACCTT CGAGTGCAGC GATGGGACTT CGTTTGCTGA GGAGGTGGAG 
AAGCCCACCA AGTGTGGCTG TGCCCAGTGC GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge.
Cell reports26(6)1458-1472.e4(2019 02)
Dun XP,Carr L,Woodley PK,Barry RW,Drake LK,Mindos T,Roberts SL,Lloyd AC,Parkinson DB

A refinement to the formalin test in mice.
F1000Research8891(2019)
Lopes DM,Cater HL,Thakur M,Wells S,McMahon SB

Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors.
Nature communications9(1)5082(2018 11)
Escot S,Willnow D,Naumann H,Di Francescantonio S,Spagnoli FM

ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-β signalling.
Nature communications9(1)5083(2018 11)
Pinho AV,Van Bulck M,Chantrill L,Arshi M,Sklyarova T,Herrmann D,Vennin C,Gallego-Ortega D,Mawson A,Giry-Laterriere M,Magenau A,Leuckx G,Baeyens L,Gill AJ,Phillips P,Timpson P,Biankin AV,Wu J,Rooman I

Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels.
Cell174(3)590-606.e21(2018 07)
Cárdenas A,Villalba A,de Juan Romero C,Picó E,Kyrousi C,Tzika AC,Tessier-Lavigne M,Ma L,Drukker M,Cappello S,Borrell V

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Slit1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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