Sema5b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema5b
Entrez Gene ID	20357
Full Name	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Synonyms	AI893641,SemG,Semag,mKIAA1445
General protein information	Preferred Names sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B Names semaphorin-5B sema G semaphorin-G
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011245845.2	XP_011244147.1	semaphorin-5B isoform X2
XM_011245847.2	XP_011244149.1	semaphorin-5B isoform X3
NM_013661.2	NP_038689.2	semaphorin-5B precursor
XM_011245844.1	XP_011244146.1	semaphorin-5B isoform X1
XM_011245847.3	XP_011244149.1	semaphorin-5B isoform X3
XM_030249006.1	XP_030104866.1	semaphorin-5B isoform X2
XM_006521869.1	XP_006521932.1	semaphorin-5B isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema5b	NP_001100561.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002424	XP_312521.5
Bos taurus (cattle)	SEMA5B	XP_002684855.1
Gallus gallus (chicken)	SEMA5B	XP_422096.2
Canis lupus familiaris (dog)	SEMA5B	XP_005639706.1
Homo sapiens (human)	SEMA5B	NP_001026872.2
Pan troglodytes (chimpanzee)	SEMA5B	XP_516697.4
Mus musculus (house mouse)	Sema5b	NP_038689.2
Macaca mulatta (Rhesus monkey)	SEMA5B	XP_002802721.1
Danio rerio (zebrafish)	sema5ba	XP_684379.3
Drosophila melanogaster (fruit fly)	Sema-5c	NP_611293.3

Gene Ontology
Bibliography

Related articles in PubMed

Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development.
Jung JS, Zhang KD, Wang Z, McMurray M, Tkaczuk A, Ogawa Y, Hertzano R, Coate TM
The Journal of neuroscience : the official journal of the Society for Neuroscience39(33)6425-6438(2019 Aug)

Balance between BDNF and Semaphorins gates the innervation of the mammary gland.
Sar Shalom H, Goldner R, Golan-Vaishenker Y, Yaron A
eLife8(2019 01)

The ciliogenic transcription factor Rfx3 is required for the formation of the thalamocortical tract by regulating the patterning of prethalamus and ventral telencephalon.
Magnani D, Morlé L, Hasenpusch-Theil K, Paschaki M, Jacoby M, Schurmans S, Durand B, Theil T
Human molecular genetics24(9)2578-93(2015 May)

Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells.
Duan Y, Wang SH, Song J, Mironova Y, Ming GL, Kolodkin AL, Giger RJ
eLife3(2014 Oct)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sema5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68667	XM_011245845.2 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu68668	XM_011245847.3 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu51938	XM_006521869.1 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu51938	XM_011245844.1 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu09754	NM_013661.2 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00
OMu09754	XM_030249006.1 Latest version!	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00
View Old Accession Versions >>
OMu68668	XM_011245847.2	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema5b ( XM_011245845.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244147.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu68667
Clone ID Related Accession (Same CDS sequence)	XM_011245845.2
Accession Version	XM_011245845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGGGCCC GGCCCTATCA CTGTGTCCCT GGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244147.1
CDS	500..2347
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPWKPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRGPALSLCPWP

Target ORF information:

RefSeq Version	XM_011245845.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245845.2

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGGGCCC GGCCCTATCA CTGTGTCCCT GGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( XM_011245847.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244149.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu68668
Clone ID Related Accession (Same CDS sequence)	XM_011245847.2 , XM_011245847.3
Accession Version	XM_011245847.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTTCTGCAGG TACGAAATGT GACACGGGAT GGGGGCTTCG GCCCATGGTC 
ACCATGGAAA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244149.1
CDS	500..2197
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPSAGTKCDTGWGLRPMVTMETV

Target ORF information:

RefSeq Version	XM_011245847.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245847.2

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTTCTGCAGG TACGAAATGT GACACGGGAT GGGGGCTTCG GCCCATGGTC 
ACCATGGAAA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( XM_011245847.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244149.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu68668
Clone ID Related Accession (Same CDS sequence)	XM_011245847.2 , XM_011245847.3
Accession Version	XM_011245847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTTCTGCAGG TACGAAATGT GACACGGGAT GGGGGCTTCG GCCCATGGTC 
ACCATGGAAA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244149.1
CDS	500..2197
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPSAGTKCDTGWGLRPMVTMETV

Target ORF information:

RefSeq Version	XM_011245847.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245847.3

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTTCTGCAGG TACGAAATGT GACACGGGAT GGGGGCTTCG GCCCATGGTC 
ACCATGGAAA CCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( XM_011245844.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244146.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X2, mRNA.

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CloneID	OMu51938
Clone ID Related Accession (Same CDS sequence)	XM_011245844.1 , XM_006521869.1
Accession Version	XM_011245844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGTGA GGTCGCGGGG CTGGGGCGCG GGGAGCGGGG AGACCTACAG TCCAGGCGGG 
GTTGGGGGGT CTGAAGCCAC CCTCACCCCG AAGGAGTTCA AGACCTGCAA CCCCGAGGCT 
TGCCCGGAAG TGCGACGCAA CACACCCTGG ACGCCCTGGC TGCCCGTGAA CGTGACCCAG 
GGTGGAGCGC GCCAGGAGCA GCGATTCCGC TTCACCTGCC GCGCGCCGCT GCCGGACCCG 
CACGGTCTGC AGTTCGGCAA GAGGAGGACA GAGACCAGGA CTTGCCCGGC AGACGGCACC 
GGAGCCTGCG ACACCGACGC CCTGGTGGAG GATCTCCTGC GCAGCGGGAG CACGTCACCA 
CACACTCTGA ACGGAGGCTG GGCCACCTGG GGCCCGTGGT CATCCTGCTC CCGGGACTGC 
GAGCTGGGCT TCCGCGTCCG CAAGAGAACT TGTACCAACC CGGAGCCTCG CAACGGGGGC 
TTGCCCTGCG TGGGAGACGC TGCGGAGTAC CAAGACTGCA ACCCACAGGC TTGCCCAGTG 
CGGGGTGCTT GGTCCTGCTG GACCGCATGG TCCCAGTGCT CAGCATCCTG CGGTGGTGGC 
CACTATCAAC GCACCCGTTC CTGCACCAGC CCCGCCCCAT CCCCAGGTGA GGACATCTGC 
CTCGGCCTGC ACACGGAGGA GGCCCTATGT TCAACACAGG CCTGCCCAGA AGGCTGGTCA 
CTGTGGTCTG AGTGGGGTGT CTGCACTGAG GATGGGGCCC AGAGCCGGAG CCGGAGCTGT 
GAGGAGCTTC TCCCAGGACC AGGTGCCTGT GTTGGCAACA GCAGCCAGAG CCGGCCCTGC 
CCCTACAGTG AGATTCCTGT CATCCTACCT GCTTCCAGTG TGGAGGAGAC CACCAGCTGT 
GGAGGGTTCA ATCTCATCCA CCTGATAGTC ACTGGTGTGT CCTGCTTCCT GGTTTCTGGG 
CTCTTGACCT TGGCAGTGTA CCTGTCTTGC CAGCACTGCC AGCGCCAGTC TCAGGAGTCC 
ACGCTTGTCC ATCCTGCCAC ACCTAACCAC TTGCACTACA AGGGTGGGGG CACCCCCAAG 
AATGAGAAGT ACACCCCTAT GGAATTCAAG ACACTGAACA AGAACAACTT AATCCCTGAT 
GACAGAGCCA ACTTCTACCC ACTGCAGCAG ACCAATGTGT ATACAACCAC GTACTACCCC 
AGCCCACTGA ACAAGCCCAG CTTCCGGCCT GAGGCCTCAC CTGGACAGCG CTGTTTCCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244146.1
CDS	587..3955
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPWKPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGCGVVRSRGWGAGSGETYSPGGVGGSEATLTPKEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPCPYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_011245844.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245844.1

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGTGA GGTCGCGGGG CTGGGGCGCG GGGAGCGGGG AGACCTACAG TCCAGGCGGG 
GTTGGGGGGT CTGAAGCCAC CCTCACCCCG AAGGAGTTCA AGACCTGCAA CCCCGAGGCT 
TGCCCGGAAG TGCGACGCAA CACACCCTGG ACGCCCTGGC TGCCCGTGAA CGTGACCCAG 
GGTGGAGCGC GCCAGGAGCA GCGATTCCGC TTCACCTGCC GCGCGCCGCT GCCGGACCCG 
CACGGTCTGC AGTTCGGCAA GAGGAGGACA GAGACCAGGA CTTGCCCGGC AGACGGCACC 
GGAGCCTGCG ACACCGACGC CCTGGTGGAG GATCTCCTGC GCAGCGGGAG CACGTCACCA 
CACACTCTGA ACGGAGGCTG GGCCACCTGG GGCCCGTGGT CATCCTGCTC CCGGGACTGC 
GAGCTGGGCT TCCGCGTCCG CAAGAGAACT TGTACCAACC CGGAGCCTCG CAACGGGGGC 
TTGCCCTGCG TGGGAGACGC TGCGGAGTAC CAAGACTGCA ACCCACAGGC TTGCCCAGTG 
CGGGGTGCTT GGTCCTGCTG GACCGCATGG TCCCAGTGCT CAGCATCCTG CGGTGGTGGC 
CACTATCAAC GCACCCGTTC CTGCACCAGC CCCGCCCCAT CCCCAGGTGA GGACATCTGC 
CTCGGCCTGC ACACGGAGGA GGCCCTATGT TCAACACAGG CCTGCCCAGA AGGCTGGTCA 
CTGTGGTCTG AGTGGGGTGT CTGCACTGAG GATGGGGCCC AGAGCCGGAG CCGGAGCTGT 
GAGGAGCTTC TCCCAGGACC AGGTGCCTGT GTTGGCAACA GCAGCCAGAG CCGGCCCTGC 
CCCTACAGTG AGATTCCTGT CATCCTACCT GCTTCCAGTG TGGAGGAGAC CACCAGCTGT 
GGAGGGTTCA ATCTCATCCA CCTGATAGTC ACTGGTGTGT CCTGCTTCCT GGTTTCTGGG 
CTCTTGACCT TGGCAGTGTA CCTGTCTTGC CAGCACTGCC AGCGCCAGTC TCAGGAGTCC 
ACGCTTGTCC ATCCTGCCAC ACCTAACCAC TTGCACTACA AGGGTGGGGG CACCCCCAAG 
AATGAGAAGT ACACCCCTAT GGAATTCAAG ACACTGAACA AGAACAACTT AATCCCTGAT 
GACAGAGCCA ACTTCTACCC ACTGCAGCAG ACCAATGTGT ATACAACCAC GTACTACCCC 
AGCCCACTGA ACAAGCCCAG CTTCCGGCCT GAGGCCTCAC CTGGACAGCG CTGTTTCCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( XM_006521869.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521932.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51938
Clone ID Related Accession (Same CDS sequence)	XM_011245844.1 , XM_006521869.1
Accession Version	XM_006521869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGTGA GGTCGCGGGG CTGGGGCGCG GGGAGCGGGG AGACCTACAG TCCAGGCGGG 
GTTGGGGGGT CTGAAGCCAC CCTCACCCCG AAGGAGTTCA AGACCTGCAA CCCCGAGGCT 
TGCCCGGAAG TGCGACGCAA CACACCCTGG ACGCCCTGGC TGCCCGTGAA CGTGACCCAG 
GGTGGAGCGC GCCAGGAGCA GCGATTCCGC TTCACCTGCC GCGCGCCGCT GCCGGACCCG 
CACGGTCTGC AGTTCGGCAA GAGGAGGACA GAGACCAGGA CTTGCCCGGC AGACGGCACC 
GGAGCCTGCG ACACCGACGC CCTGGTGGAG GATCTCCTGC GCAGCGGGAG CACGTCACCA 
CACACTCTGA ACGGAGGCTG GGCCACCTGG GGCCCGTGGT CATCCTGCTC CCGGGACTGC 
GAGCTGGGCT TCCGCGTCCG CAAGAGAACT TGTACCAACC CGGAGCCTCG CAACGGGGGC 
TTGCCCTGCG TGGGAGACGC TGCGGAGTAC CAAGACTGCA ACCCACAGGC TTGCCCAGTG 
CGGGGTGCTT GGTCCTGCTG GACCGCATGG TCCCAGTGCT CAGCATCCTG CGGTGGTGGC 
CACTATCAAC GCACCCGTTC CTGCACCAGC CCCGCCCCAT CCCCAGGTGA GGACATCTGC 
CTCGGCCTGC ACACGGAGGA GGCCCTATGT TCAACACAGG CCTGCCCAGA AGGCTGGTCA 
CTGTGGTCTG AGTGGGGTGT CTGCACTGAG GATGGGGCCC AGAGCCGGAG CCGGAGCTGT 
GAGGAGCTTC TCCCAGGACC AGGTGCCTGT GTTGGCAACA GCAGCCAGAG CCGGCCCTGC 
CCCTACAGTG AGATTCCTGT CATCCTACCT GCTTCCAGTG TGGAGGAGAC CACCAGCTGT 
GGAGGGTTCA ATCTCATCCA CCTGATAGTC ACTGGTGTGT CCTGCTTCCT GGTTTCTGGG 
CTCTTGACCT TGGCAGTGTA CCTGTCTTGC CAGCACTGCC AGCGCCAGTC TCAGGAGTCC 
ACGCTTGTCC ATCCTGCCAC ACCTAACCAC TTGCACTACA AGGGTGGGGG CACCCCCAAG 
AATGAGAAGT ACACCCCTAT GGAATTCAAG ACACTGAACA AGAACAACTT AATCCCTGAT 
GACAGAGCCA ACTTCTACCC ACTGCAGCAG ACCAATGTGT ATACAACCAC GTACTACCCC 
AGCCCACTGA ACAAGCCCAG CTTCCGGCCT GAGGCCTCAC CTGGACAGCG CTGTTTCCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521932.1
CDS	500..3868
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPWKPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGCGVVRSRGWGAGSGETYSPGGVGGSEATLTPKEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPCPYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_006521869.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521869.1

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGTGA GGTCGCGGGG CTGGGGCGCG GGGAGCGGGG AGACCTACAG TCCAGGCGGG 
GTTGGGGGGT CTGAAGCCAC CCTCACCCCG AAGGAGTTCA AGACCTGCAA CCCCGAGGCT 
TGCCCGGAAG TGCGACGCAA CACACCCTGG ACGCCCTGGC TGCCCGTGAA CGTGACCCAG 
GGTGGAGCGC GCCAGGAGCA GCGATTCCGC TTCACCTGCC GCGCGCCGCT GCCGGACCCG 
CACGGTCTGC AGTTCGGCAA GAGGAGGACA GAGACCAGGA CTTGCCCGGC AGACGGCACC 
GGAGCCTGCG ACACCGACGC CCTGGTGGAG GATCTCCTGC GCAGCGGGAG CACGTCACCA 
CACACTCTGA ACGGAGGCTG GGCCACCTGG GGCCCGTGGT CATCCTGCTC CCGGGACTGC 
GAGCTGGGCT TCCGCGTCCG CAAGAGAACT TGTACCAACC CGGAGCCTCG CAACGGGGGC 
TTGCCCTGCG TGGGAGACGC TGCGGAGTAC CAAGACTGCA ACCCACAGGC TTGCCCAGTG 
CGGGGTGCTT GGTCCTGCTG GACCGCATGG TCCCAGTGCT CAGCATCCTG CGGTGGTGGC 
CACTATCAAC GCACCCGTTC CTGCACCAGC CCCGCCCCAT CCCCAGGTGA GGACATCTGC 
CTCGGCCTGC ACACGGAGGA GGCCCTATGT TCAACACAGG CCTGCCCAGA AGGCTGGTCA 
CTGTGGTCTG AGTGGGGTGT CTGCACTGAG GATGGGGCCC AGAGCCGGAG CCGGAGCTGT 
GAGGAGCTTC TCCCAGGACC AGGTGCCTGT GTTGGCAACA GCAGCCAGAG CCGGCCCTGC 
CCCTACAGTG AGATTCCTGT CATCCTACCT GCTTCCAGTG TGGAGGAGAC CACCAGCTGT 
GGAGGGTTCA ATCTCATCCA CCTGATAGTC ACTGGTGTGT CCTGCTTCCT GGTTTCTGGG 
CTCTTGACCT TGGCAGTGTA CCTGTCTTGC CAGCACTGCC AGCGCCAGTC TCAGGAGTCC 
ACGCTTGTCC ATCCTGCCAC ACCTAACCAC TTGCACTACA AGGGTGGGGG CACCCCCAAG 
AATGAGAAGT ACACCCCTAT GGAATTCAAG ACACTGAACA AGAACAACTT AATCCCTGAT 
GACAGAGCCA ACTTCTACCC ACTGCAGCAG ACCAATGTGT ATACAACCAC GTACTACCCC 
AGCCCACTGA ACAAGCCCAG CTTCCGGCCT GAGGCCTCAC CTGGACAGCG CTGTTTCCCC 
AACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( NM_013661.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038689.2 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), mRNA.

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CloneID	OMu09754
Clone ID Related Accession (Same CDS sequence)	NM_013661.2 , XM_030249006.1
Accession Version	NM_013661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052397.1 and AK129362.1. On Jan 15, 2008 this sequence version replaced NM_013661.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X97818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGAGT TCAAGACCTG CAACCCCGAG GCTTGCCCGG AAGTGCGACG CAACACACCC 
TGGACGCCCT GGCTGCCCGT GAACGTGACC CAGGGTGGAG CGCGCCAGGA GCAGCGATTC 
CGCTTCACCT GCCGCGCGCC GCTGCCGGAC CCGCACGGTC TGCAGTTCGG CAAGAGGAGG 
ACAGAGACCA GGACTTGCCC GGCAGACGGC ACCGGAGCCT GCGACACCGA CGCCCTGGTG 
GAGGATCTCC TGCGCAGCGG GAGCACGTCA CCACACACTC TGAACGGAGG CTGGGCCACC 
TGGGGCCCGT GGTCATCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACTTGTACCA ACCCGGAGCC TCGCAACGGG GGCTTGCCCT GCGTGGGAGA CGCTGCGGAG 
TACCAAGACT GCAACCCACA GGCTTGCCCA GTGCGGGGTG CTTGGTCCTG CTGGACCGCA 
TGGTCCCAGT GCTCAGCATC CTGCGGTGGT GGCCACTATC AACGCACCCG TTCCTGCACC 
AGCCCCGCCC CATCCCCAGG TGAGGACATC TGCCTCGGCC TGCACACGGA GGAGGCCCTA 
TGTTCAACAC AGGCCTGCCC AGAAGGCTGG TCACTGTGGT CTGAGTGGGG TGTCTGCACT 
GAGGATGGGG CCCAGAGCCG GAGCCGGAGC TGTGAGGAGC TTCTCCCAGG ACCAGGTGCC 
TGTGTTGGCA ACAGCAGCCA GAGCCGGCCC TGCCCCTACA GTGAGATTCC TGTCATCCTA 
CCTGCTTCCA GTGTGGAGGA GACCACCAGC TGTGGAGGGT TCAATCTCAT CCACCTGATA 
GTCACTGGTG TGTCCTGCTT CCTGGTTTCT GGGCTCTTGA CCTTGGCAGT GTACCTGTCT 
TGCCAGCACT GCCAGCGCCA GTCTCAGGAG TCCACGCTTG TCCATCCTGC CACACCTAAC 
CACTTGCACT ACAAGGGTGG GGGCACCCCC AAGAATGAGA AGTACACCCC TATGGAATTC 
AAGACACTGA ACAAGAACAA CTTAATCCCT GATGACAGAG CCAACTTCTA CCCACTGCAG 
CAGACCAATG TGTATACAAC CACGTACTAC CCCAGCCCAC TGAACAAGCC CAGCTTCCGG 
CCTGAGGCCT CACCTGGACA GCGCTGTTTC CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038689.2
CDS	292..3573
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPWKPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPCPYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_013661.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013661.2

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGAGT TCAAGACCTG CAACCCCGAG GCTTGCCCGG AAGTGCGACG CAACACACCC 
TGGACGCCCT GGCTGCCCGT GAACGTGACC CAGGGTGGAG CGCGCCAGGA GCAGCGATTC 
CGCTTCACCT GCCGCGCGCC GCTGCCGGAC CCGCACGGTC TGCAGTTCGG CAAGAGGAGG 
ACAGAGACCA GGACTTGCCC GGCAGACGGC ACCGGAGCCT GCGACACCGA CGCCCTGGTG 
GAGGATCTCC TGCGCAGCGG GAGCACGTCA CCACACACTC TGAACGGAGG CTGGGCCACC 
TGGGGCCCGT GGTCATCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACTTGTACCA ACCCGGAGCC TCGCAACGGG GGCTTGCCCT GCGTGGGAGA CGCTGCGGAG 
TACCAAGACT GCAACCCACA GGCTTGCCCA GTGCGGGGTG CTTGGTCCTG CTGGACCGCA 
TGGTCCCAGT GCTCAGCATC CTGCGGTGGT GGCCACTATC AACGCACCCG TTCCTGCACC 
AGCCCCGCCC CATCCCCAGG TGAGGACATC TGCCTCGGCC TGCACACGGA GGAGGCCCTA 
TGTTCAACAC AGGCCTGCCC AGAAGGCTGG TCACTGTGGT CTGAGTGGGG TGTCTGCACT 
GAGGATGGGG CCCAGAGCCG GAGCCGGAGC TGTGAGGAGC TTCTCCCAGG ACCAGGTGCC 
TGTGTTGGCA ACAGCAGCCA GAGCCGGCCC TGCCCCTACA GTGAGATTCC TGTCATCCTA 
CCTGCTTCCA GTGTGGAGGA GACCACCAGC TGTGGAGGGT TCAATCTCAT CCACCTGATA 
GTCACTGGTG TGTCCTGCTT CCTGGTTTCT GGGCTCTTGA CCTTGGCAGT GTACCTGTCT 
TGCCAGCACT GCCAGCGCCA GTCTCAGGAG TCCACGCTTG TCCATCCTGC CACACCTAAC 
CACTTGCACT ACAAGGGTGG GGGCACCCCC AAGAATGAGA AGTACACCCC TATGGAATTC 
AAGACACTGA ACAAGAACAA CTTAATCCCT GATGACAGAG CCAACTTCTA CCCACTGCAG 
CAGACCAATG TGTATACAAC CACGTACTAC CCCAGCCCAC TGAACAAGCC CAGCTTCCGG 
CCTGAGGCCT CACCTGGACA GCGCTGTTTC CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5b ( XM_030249006.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104866.1 Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X3, mRNA.

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CloneID	OMu09754
Clone ID Related Accession (Same CDS sequence)	NM_013661.2 , XM_030249006.1
Accession Version	XM_030249006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGAGT TCAAGACCTG CAACCCCGAG GCTTGCCCGG AAGTGCGACG CAACACACCC 
TGGACGCCCT GGCTGCCCGT GAACGTGACC CAGGGTGGAG CGCGCCAGGA GCAGCGATTC 
CGCTTCACCT GCCGCGCGCC GCTGCCGGAC CCGCACGGTC TGCAGTTCGG CAAGAGGAGG 
ACAGAGACCA GGACTTGCCC GGCAGACGGC ACCGGAGCCT GCGACACCGA CGCCCTGGTG 
GAGGATCTCC TGCGCAGCGG GAGCACGTCA CCACACACTC TGAACGGAGG CTGGGCCACC 
TGGGGCCCGT GGTCATCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACTTGTACCA ACCCGGAGCC TCGCAACGGG GGCTTGCCCT GCGTGGGAGA CGCTGCGGAG 
TACCAAGACT GCAACCCACA GGCTTGCCCA GTGCGGGGTG CTTGGTCCTG CTGGACCGCA 
TGGTCCCAGT GCTCAGCATC CTGCGGTGGT GGCCACTATC AACGCACCCG TTCCTGCACC 
AGCCCCGCCC CATCCCCAGG TGAGGACATC TGCCTCGGCC TGCACACGGA GGAGGCCCTA 
TGTTCAACAC AGGCCTGCCC AGAAGGCTGG TCACTGTGGT CTGAGTGGGG TGTCTGCACT 
GAGGATGGGG CCCAGAGCCG GAGCCGGAGC TGTGAGGAGC TTCTCCCAGG ACCAGGTGCC 
TGTGTTGGCA ACAGCAGCCA GAGCCGGCCC TGCCCCTACA GTGAGATTCC TGTCATCCTA 
CCTGCTTCCA GTGTGGAGGA GACCACCAGC TGTGGAGGGT TCAATCTCAT CCACCTGATA 
GTCACTGGTG TGTCCTGCTT CCTGGTTTCT GGGCTCTTGA CCTTGGCAGT GTACCTGTCT 
TGCCAGCACT GCCAGCGCCA GTCTCAGGAG TCCACGCTTG TCCATCCTGC CACACCTAAC 
CACTTGCACT ACAAGGGTGG GGGCACCCCC AAGAATGAGA AGTACACCCC TATGGAATTC 
AAGACACTGA ACAAGAACAA CTTAATCCCT GATGACAGAG CCAACTTCTA CCCACTGCAG 
CAGACCAATG TGTATACAAC CACGTACTAC CCCAGCCCAC TGAACAAGCC CAGCTTCCGG 
CCTGAGGCCT CACCTGGACA GCGCTGTTTC CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104866.1
CDS	587..3868
Translation	MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFMCGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRVLRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPWKPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPCPYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_030249006.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249006.1

ORF Insert Sequence:

ATGGTAGTCC CAGGACCCCT GGCTCTCTCG CTGTTGCTGT CCAGCCTCAC CCTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGACATTGCC AGTGAGTCCA GCAGTGAGCA ACAGATGTGC 
ACGCGGAGGG AGCACCCCAT CGTGGCCTTT GAAGATCTGA AGCCGTGGGT CTTTAACTTC 
ACCTACCCTG GAGTCCGGGA TTTCTCCCAG CTTGCTCTTG ATCCCTCGAG GAATCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTCG CCAATGTCTC CCTCCTTCAG 
GCTACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAGAGCAA AGGGAAAACG 
GAGGAGGAGT GTCAGAACTA TGTACGAGTC CTGATTGTTT CCGGCCGGAA GGTGTTCATG 
TGCGGTACCA ATGCCTTTTC CCCAGTGTGC TCCAGCAGAC AGGTAGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAACGG TGTGGCCCGG TGCCCCTATG ACCCACGCCA CAACTCTACA 
GCCGTCATCT CCTCTCAGGG GGAGCTCTAT GCAGCCACAG TCATTGACTT CTCCGGTCGG 
GACCCAGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAGTATAAC 
TCCAAATGGC TCAATGAGCC AAATTTTGTG GCAGCCTTTG ACATCGGGCT GTTTGCGTAT 
TTCTTCCTTC GGGAGAATGC CGTGGAGCAT GATTGTGGGC GCACTGTGTA CTCTCGGGTG 
GCCCGGGTGT GCAAGAATGA TGTAGGTGGC CGTTTCCTGC TGGAGGACAC GTGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGAGAGG TCCCCTTCTA CTATAATGAG 
CTGCAGAGTG CCTTCCATCT GCCCGAGCAG GACCTCATCT ATGGCGTCTT CACCACTAAC 
GTAAACAGCA TTGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCCAT CTCCAAGGCT 
TTCAATGGCC CATTTCGTTA CCAGGAAAAC CCCAGGGCTG CCTGGCTCCC AATCGCCAAT 
CCCATCCCCA ATTTCCAGTG TGGCACTCTG CCGGAGACTG GCCCCAACGA GAACCTCACG 
GAGCGCAGCC TGCAGGACGC ACAGCGGCTC TTCCTGATGA GCGAAGCTGT GCAGCCAGTG 
ACACCAGAGC CCTGTGTCAC CCAGGACAGC GTCCGCTTCT CACATCTCGT GGTAGACCTT 
GTGCAAGCTA AGGACACGCT CTATCACGTA CTCTACATAG GCACGGAGTC GGGTACCATC 
CTGAAAGCGC TGTCCACGGC CAGCCGCAGC CTCCGTGGCT GCTACCTGGA GGAGCTACAT 
GTGCTGCCTC CTGGGCGCCT TGAACCTCTG CGGAGCCTGC GCATCCTGCA CAGCGCGCGT 
GCGCTCTTCG TGGGATTGAG CGACAGGGTG CTGCGGGTCC CACTGGAGAG GTGTTCGGCC 
TATCATAGCC AGGGGGCATG CCTGGGAGCA CGGGACCCAT ACTGCGGCTG GGATGGGAAG 
CGGCAACTTT GCAGCACGCT TGAAGACAGT TCCAACATGA GCCTGTGGAT CCAGAACATC 
ACAACCTGTC CTGTACGAAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
AAACCGTGTG AGCACTTAGA TGGAGACAAC TCGGGTTCTT GCCTGTGCCG GGCCAGATCC 
TGTGACTCCC CAAGGCCTCG CTGTGGGGGC CTCGAGTGCC TGGGGCCATC CATCCATATT 
GCCAATTGCT CCAGGAATGG GGCGTGGACC GCATGGTCAT CGTGGGCTCA GTGCAGCACG 
TCCTGTGGGA TCGGCTTCCA GGTCCGTCAG CGAAGTTGCA GCAACCCGGC GCCCCGCCAT 
GGGGGCCGCA TTTGCGTGGG CAAGAGCCGG GAGGAGCGGT TCTGTAATGA AAATACACCT 
TGCCCAGTGC CCATCTTCTG GGCTTCCTGG GGTTCCTGGA GCAAGTGCAG CAACAACTGT 
GGAGGCGGCG TGCAGTCGCG ACGCCGTTCT TGCGAGAATG GCAACTCGTG CCCGGGTTGC 
GGCGTGGAGT TCAAGACCTG CAACCCCGAG GCTTGCCCGG AAGTGCGACG CAACACACCC 
TGGACGCCCT GGCTGCCCGT GAACGTGACC CAGGGTGGAG CGCGCCAGGA GCAGCGATTC 
CGCTTCACCT GCCGCGCGCC GCTGCCGGAC CCGCACGGTC TGCAGTTCGG CAAGAGGAGG 
ACAGAGACCA GGACTTGCCC GGCAGACGGC ACCGGAGCCT GCGACACCGA CGCCCTGGTG 
GAGGATCTCC TGCGCAGCGG GAGCACGTCA CCACACACTC TGAACGGAGG CTGGGCCACC 
TGGGGCCCGT GGTCATCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACTTGTACCA ACCCGGAGCC TCGCAACGGG GGCTTGCCCT GCGTGGGAGA CGCTGCGGAG 
TACCAAGACT GCAACCCACA GGCTTGCCCA GTGCGGGGTG CTTGGTCCTG CTGGACCGCA 
TGGTCCCAGT GCTCAGCATC CTGCGGTGGT GGCCACTATC AACGCACCCG TTCCTGCACC 
AGCCCCGCCC CATCCCCAGG TGAGGACATC TGCCTCGGCC TGCACACGGA GGAGGCCCTA 
TGTTCAACAC AGGCCTGCCC AGAAGGCTGG TCACTGTGGT CTGAGTGGGG TGTCTGCACT 
GAGGATGGGG CCCAGAGCCG GAGCCGGAGC TGTGAGGAGC TTCTCCCAGG ACCAGGTGCC 
TGTGTTGGCA ACAGCAGCCA GAGCCGGCCC TGCCCCTACA GTGAGATTCC TGTCATCCTA 
CCTGCTTCCA GTGTGGAGGA GACCACCAGC TGTGGAGGGT TCAATCTCAT CCACCTGATA 
GTCACTGGTG TGTCCTGCTT CCTGGTTTCT GGGCTCTTGA CCTTGGCAGT GTACCTGTCT 
TGCCAGCACT GCCAGCGCCA GTCTCAGGAG TCCACGCTTG TCCATCCTGC CACACCTAAC 
CACTTGCACT ACAAGGGTGG GGGCACCCCC AAGAATGAGA AGTACACCCC TATGGAATTC 
AAGACACTGA ACAAGAACAA CTTAATCCCT GATGACAGAG CCAACTTCTA CCCACTGCAG 
CAGACCAATG TGTATACAAC CACGTACTAC CCCAGCCCAC TGAACAAGCC CAGCTTCCGG 
CCTGAGGCCT CACCTGGACA GCGCTGTTTC CCCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience39(33)6425-6438(2019 Aug)
Jung JS,Zhang KD,Wang Z,McMurray M,Tkaczuk A,Ogawa Y,Hertzano R,Coate TM

Balance between BDNF and Semaphorins gates the innervation of the mammary gland.
eLife8(2019 01)
Sar Shalom H,Goldner R,Golan-Vaishenker Y,Yaron A

The ciliogenic transcription factor Rfx3 is required for the formation of the thalamocortical tract by regulating the patterning of prethalamus and ventral telencephalon.
Human molecular genetics24(9)2578-93(2015 May)
Magnani D,Morlé L,Hasenpusch-Theil K,Paschaki M,Jacoby M,Schurmans S,Durand B,Theil T

Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells.
eLife3(2014 Oct)
Duan Y,Wang SH,Song J,Mironova Y,Ming GL,Kolodkin AL,Giger RJ

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

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Sema5b cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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