Scn5a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scn5a
Entrez Gene ID	20271
Full Name	sodium channel, voltage-gated, type V, alpha
Synonyms	Nav1.5,Nav1.5c,SkM1,SkM2,mH1
General protein information	Preferred Names sodium channel, voltage-gated, type V, alpha Names sodium channel protein type 5 subunit alpha sodium channel protein cardiac muscle subunit alpha sodium channel protein type V subunit alpha sodium channel, voltage-gated, type V, alpha polypeptide voltage-gated sodium channel Nav1.5 variant a voltage-gated sodium channel Nav1.5 variant b voltage-gated sodium channel subunit alpha Nav1.5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F3\|9 71.33 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511997.1	XP_006512060.1	sodium channel protein type 5 subunit alpha isoform X4
XM_006511993.1	XP_006512056.1	sodium channel protein type 5 subunit alpha isoform X1
XM_006511995.1	XP_006512058.1	sodium channel protein type 5 subunit alpha isoform X2
XM_006511996.1	XP_006512059.1	sodium channel protein type 5 subunit alpha isoform X3
NM_021544.4	NP_067519.2	sodium channel protein type 5 subunit alpha isoform 1
NM_001253860.1	NP_001240789.1	sodium channel protein type 5 subunit alpha isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	scn12ab	NP_001038588.1
Homo sapiens (human)	SCN5A	NP_932173.1
Pan troglodytes (chimpanzee)	SCN5A	XP_001171891.2
Macaca mulatta (Rhesus monkey)	LOC695085	XP_002808375.1
Canis lupus familiaris (dog)	SCN5A	NP_001002994.1
Danio rerio (zebrafish)	scn12aa	NP_001038387.1
Rattus norvegicus (Norway rat)	Scn5a	NP_037257.1
Bos taurus (cattle)	SCN5A	NP_776883.1
Mus musculus (house mouse)	Scn5a	NP_067519.2
Gallus gallus (chicken)	SCN5A	XP_001232818.3
Xenopus tropicalis (tropical clawed frog)	scn5a	XP_002932534.2

Gene Ontology
Bibliography

Related articles in PubMed

An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo.
Man JCK, Mohan RA, Boogaard MVD, Hilvering CRE, Jenkins C, Wakker V, Bianchi V, Laat W, Barnett P, Boukens BJ, Christoffels VM
Nature communications10(1)4943(2019 10)

Autonomic modulation of the electrical substrate in mice haploinsufficient for cardiac sodium channels: a model of the Brugada syndrome.
Finlay M, Bhar-Amato J, Ng KE, Santos D, Orini M, Vyas V, Taggart P, Grace AA, Huang CL, Lambiase PD, Tinker A
American journal of physiology. Cell physiology317(3)C576-C583(2019 Sep)

Protein Phosphatase 2A Regulates Cardiac Na
El Refaey M, Musa H, Murphy NP, Lubbers ER, Skaf M, Han M, Cavus O, Koenig SN, Wallace MJ, Gratz D, Bradley E, Alsina KM, Wehrens XHT, Hund TJ, Mohler PJ
Circulation research124(5)737-746(2019 03)

Gene-environment interaction impacts on heart development and embryo survival.
Moreau JLM, Kesteven S, Martin EMMA, Lau KS, Yam MX, O'Reilly VC, Del Monte-Nieto G, Baldini A, Feneley MP, Moon AM, Harvey RP, Sparrow DB, Chapman G, Dunwoodie SL
Development (Cambridge, England)146(4)(2019 02)

CRISPR -Mediated Expression of the Fetal Scn5a Isoform in Adult Mice Causes Conduction Defects and Arrhythmias.
Pang PD, Alsina KM, Cao S, Koushik AB, Wehrens XHT, Cooper TA
Journal of the American Heart Association7(19)e010393(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Scn5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scn5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19312	NM_001253860.1 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu19341	NM_021544.4 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu19312	XM_006511997.1 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu43337	XM_006511993.1 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu43339	XM_006511995.1 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu43340	XM_006511996.1 Latest version!	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scn5a ( NM_001253860.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001240789.1 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19312
Clone ID Related Accession (Same CDS sequence)	XM_006511997.1 , NM_001253860.1
Accession Version	NM_001253860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147254.1, AC121922.3 and AK085822.1. Transcript Variant: This variant (2) switches an exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is the same length as isoform 1 yet it has a different sequence. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CATACACAAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC CTTACGCACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCGGTCATTT CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240789.1
CDS	55..6117
Translation	MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	NM_001253860.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253860.1

ORF Insert Sequence:

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CATACACAAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC CTTACGCACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCGGTCATTT CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( NM_021544.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067519.2 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 1, mRNA.

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CloneID	OMu19341
Clone ID Related Accession (Same CDS sequence)	NM_021544.4
Accession Version	NM_021544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577030400000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147254.1 and AK085822.1. On Dec 8, 2011 this sequence version replaced NM_021544.3. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147254.1, AK147517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CGTATGTATC AGAGAATATA AAGCTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT GAAAACGATT TCAGTTATTC CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067519.2
CDS	223..6285
Translation	MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	NM_021544.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021544.4

ORF Insert Sequence:

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CGTATGTATC AGAGAATATA AAGCTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT GAAAACGATT TCAGTTATTC CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_006511997.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512060.1 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X4, mRNA.

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CloneID	OMu19312
Clone ID Related Accession (Same CDS sequence)	XM_006511997.1 , NM_001253860.1
Accession Version	XM_006511997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CATACACAAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC CTTACGCACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCGGTCATTT CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512060.1
CDS	237..6299
Translation	MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_006511997.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511997.1

ORF Insert Sequence:

ATGGCAAACT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG CATGGCTGAA AAACAAGCCC GTGGTTCGGC CACCTCACAG 
GAGAGCCGTG AGGGCCTGCC AGAGGAGGAG GCTCCCCGGC CCCAGCTGGA CCTACAGGCC 
TCCAAAAAGC TGCCAGATCT CTATGGCAAC CCACCCCGAG AGCTCATTGG GGAGCCCCTG 
GAAGACCTGG ACCCTTTCTA TAGTACCCAG AAGACCTTCA TCGTGCTGAA TAAGGGCAAA 
ACCATCTTCC GGTTCAGTGC CACCAATGCC TTGTACGTCC TCAGCCCCTT CCACCCGGTG 
CGCAGAGCGG CTGTGAAGAT TTTGGTACAT TCGCTCTTCA GCATGCTCAT CATGTGCACC 
ATCCTAACCA ACTGTGTGTT CATGGCACAA CACGACCCTC CGCCTTGGAC CAAATATGTT 
GAGTACACCT TCACCGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT AGCTCGAGGC 
TTCTGCCTGC ATGCGTTCAC CTTCCTCCGG GACCCGTGGA ACTGGCTAGA CTTCAGTGTG 
ATCGTCATGG CATACACAAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC CTTACGCACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCGGTCATTT CAGGCCTGAA GACAATCGTG 
GGAGCCCTAA TCCAGTCTGT GAAGAAGCTA GCCGATGTGA TGGTCCTTAC TGTCTTCTGC 
CTCAGTGTCT TTGCCCTCAT TGGCCTGCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTGCGTAACT TTACTGAGCT CAATGGCACC AATGGTTCCG TGGAGGCCGA TGGCATAGTC 
TGGAACTCTC TGGACGTCTA CCTCAATGAC CCAGCCAATT ACCTACTCAA GAATGGCACC 
ACGGATGTGT TGCTGTGTGG GAACAGCTCT GATGCCGGGA CATGTCCTGA AGGCTACCGG 
TGCCTGAAGG CAGGTGAGAA CCCTGACCAC GGTTACACCA GCTTCGACTC CTTCGCCTGG 
GCTTTCCTTG CGCTCTTCCG CCTGATGACA CAGGACTGCT GGGAACGTCT ATACCAGCAG 
ACCCTGAGGT CCGCAGGAAA GATCTACATG ATCTTCTTCA TGCTTGTCAT ATTCCTGGGC 
TCCTTCTACC TTGTGAACTT GATCCTGGCT GTGGTGGCCA TGGCTTACGA GGAGCAAAAC 
CAAGCCACCA TCGCCGAGAC GGAAGAGAAG GAGAAGCGTT TCCAGGAGGC CATGGAGATG 
CTCAAGAAGG AACACGAGGC TCTCACCATC AGGGGCGTGG ATACTGTGTC CCGTAGCTCT 
CTGGAGATGT CTCCTTTGGC CCCAGTAACC AACCATGAGA GAAGGAGCAA GAGGAGGAAA 
CGACTGTCTT CAGGGACAGA GGATGGCGGA GATGACAGGC TCCCCAAGTC GGACTCAGAA 
GACGGTCCCA GAGCATTGAA TCAGCTCAGC CTCACCCATG GGCTCAGCCG GACATCCATG 
AGGCCCCGCT CCAGCCGAGG GAGCATTTTC ACATTCCGGC GACGGGACCA AGGCTCCGAG 
GCAGATTTTG CAGATGACGA GAACAGTACA GCAGGAGAGA GCGAGAGCCA CCGCACATCG 
CTGCTGGTGC CCTGGCCCCT GCGCCGTCCC AGCACCCAAG GGCAGCCCGG CTTTGGAACC 
TCAGCTCCTG GTCATGTCCT CAATGGCAAA AGAAACAGCA CCGTGGACTG CAATGGGGTG 
GTTTCCTTGC TGGGGGCAGG GGACGCAGAG GCCACCTCTC CAGGGAGCCA CCTTCTCCGC 
CCTATAGTGC TGGACAGACC CCCAGACACG ACCACGCCGT CAGAGGAGCC GGGTGGGCCC 
CAGATGCTGA CACCGCAGGC TCCATGTGCA GATGGCTTTG AGGAGCCCGG GGCACGGCAA 
CGGGCACTCA GCGCCGTCAG TGTCCTCACC AGCGCTCTGG AAGAGTTGGA GGAGTCCCAC 
CGTAAGTGTC CACCATGCTG GAATCGCTTT GCCCAGCATT ACCTCATCTG GGAGTGCTGC 
CCACTCTGGA TGTCCATCAA GCAGAAGGTG AAGTTCGTGG TCATGGACCC GTTTGCTGAC 
CTCACCATCA CCATGTGCAT CGTGCTCAAC ACGCTCTTCA TGGCTCTGGA GCACTACAAC 
ATGACAGCCG AGTTTGAGGA GATGCTGCAG GTCGGAAACT TGGTCTTCAC GGGAATCTTC 
ACGGCAGAGA TGACCTTCAA GATCATCGCC CTTGACCCAT ACTACTACTT CCAGCAGGGC 
TGGAATATCT TCGACAGCAT CATCGTCATC CTCAGCCTCA TGGAGCTGGG CCTGTCTCGC 
ATGGGCAACC TGTCTGTGCT GCGTTCCTTC CGTCTGCTGC GGGTCTTCAA GCTGGCCAAA 
TCATGGCCCA CCCTGAACAC ACTCATCAAG ATCATCGGGA ACTCTGTTGG TGCCCTGGGG 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATCTTCG CCGTGGTGGG TATGCAGCTC 
TTCGGCAAGA ACTACTCAGA GCTGAGGCAC CGAATCAGCG ACTCTGGCCT GCTGCCCCGC 
TGGCACATGA TGGACTTCTT CCACGCCTTC CTCATCATCT TCCGCATCCT CTGTGGAGAG 
TGGATCGAGA CCATGTGGGA CTGCATGGAG GTGTCTGGGC AGTCGCTGTG CTTGCTGGTC 
TTCCTGCTGG TCATGGTCAT TGGCAACCTT GTGGTCCTGA ATCTCTTCTT GGCCTTGCTG 
CTCAGCTCCT TCAGCGCAGA CAACCTCACA GCCCCTGACG AGGATGGGGA GATGAACAAC 
CTCCAGCTGG CCCTGGCTCG CATCCAAAGG GGCCTTCGCT TTGTCAAGCG GACCACCTGG 
GACTTCTGCT GCGGGCTCCT GCGGCGGCGA CCTAAGAAGC CCGCGGCTCT TGCCACCCAC 
AGCCAGCTGC CCAGCTGTAT CGCCGCTCCC AGGTCCCCAC CACCCCCAGA GGTGGAGAAG 
GCGCCCCCAG CCCGCAAGGA AACACGGTTC GAGGAAGACA AGCGGCCGGG CCAGGGCACC 
CCTGGGGATA CCGAGCCTGT GTGTGTTCCC ATCGCAGTGG CTGAGTCAGA CACTGATGAC 
CAGGAAGAGG ATGAGGAGAA CAGCCTTGGC ACGGAGGAGG AAGAGTCCAG CAAACAGCAG 
GAATCCCAAG TTGTGTCTGG TGGCCACGAG CCCCCCCAGG AGCCCAGGGC CTGGAGCCAG 
GTGTCAGAGA CCACATCCTC TGAAGCCGAG GCCAGTACGT CTCAGGCAGA CTGGCAGCAA 
GAGCGGGAAG CGGAGCCCCG GGCCCCGGGG TGCGGTGAGA CTCCCGAGGA CAGTTACTCC 
GAGGGTAGCA CCGCTGACAT GACCAACACC GCAGACCTCC TGGAGCAAAT CCCAGACCTC 
GGGGAGGACG TCAAGGACCC AGAGGACTGC TTCACTGAAG GCTGCGTCCG ACGTTGTCCT 
TGCTGCATGG TGGACACGAC GCAGGCCCCA GGGAAGGTCT GGTGGAGACT GCGCAAGACC 
TGCTACCGCA TAGTGGAGCA CAGCTGGTTC GAGACTTTCA TCATCTTCAT GATCCTGCTC 
AGCAGCGGAG CGCTGGCCTT CGAGGACATC TACCTGGAGG AGCGGAAGAC CATCAAGGTT 
CTGCTGGAGT ACGCCGACAA GATGTTCACC TATGTCTTTG TGTTGGAGAT GCTGCTCAAG 
TGGGTGGCCT ACGGCTTCAA GAAGTACTTC ACCAACGCCT GGTGCTGGCT GGACTTCCTA 
ATTGTGGACG TCTCACTGGT CAGCCTCGTG GCCAACACCT TAGGCTTTGC CGAGATGGGT 
CCCATCAAGT CCCTGAGGAC ACTGCGTGCA CTTCGACCCC TGAGGGCTTT GTCACGATTC 
GAGGGCATGA GGGTGGTGGT CAACGCGCTG GTGGGCGCCA TCCCCTCCAT CATGAATGTC 
CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTGAA CCTCTTCGCC 
GGGAAGTTCG GTAGGTGCAT CAACCAGACC GAAGGGGACC TGCCTCTGAA CTACACCATT 
GTGAACAACA AGAGCGAGTG TGAGTCCTTC AACGTGACTG GAGAGTTGTA CTGGACCAAG 
GTGAAAGTCA ACTTTGACAA CGTGGGGGCT GGGTACCTGG CCCTGCTGCA GGTGGCGACA 
TTTAAAGGCT GGATGGACAT CATGTATGCG GCTGTGGACT CCAGAGGGTA TGAAGAGCAG 
CCGCAGTGGG AGGACAACCT CTACATGTAC ATCTACTTCG TCGTCTTCAT CATCTTCGGC 
TCCTTCTTCA CCCTCAACCT GTTTATTGGT GTCATCATTG ATAACTTCAA CCAGCAGAAG 
AAAAAGTTAG GGGGCCAGGA CATCTTCATG ACGGAGGAGC AGAAGAAGTA CTACAATGCC 
ATGAAGAAGC TGGGCTCCAA GAAACCCCAG AAGCCCATCC CACGGCCTCT GAACAAGTAC 
CAGGGTTTCA TATTCGACAT TGTGACCAAG CAGGCCTTCG ATGTTACCAT CATGTTCCTC 
ATCTGTTTAA ACATGGTGAC CATGATGGTA GAGACAGATG ATCAGAGCCC TGAAAAAGTC 
AACATCTTGG CCAAGATCAA CCTGCTCTTC GTGGCCATCT TCACAGGAGA GTGTATTGTC 
AAGATGGCTG CCCTGCGCCA CTATTACTTC ACCAACAGCT GGAACATCTT CGATTTCGTG 
GTTGTCATCC TCTCCATCGT TGGCACTGTC CTCTCCGACA TCATCCAGAA GTACTTCTTC 
TCCCCAACGC TCTTCCGTGT CATCCGTCTG GCCAGGATCG GCCGCATCCT CAGGCTGATC 
CGAGGAGCCA AGGGGATTCG CACGTTGCTC TTCGCCCTCA TGATGTCCCT GCCCGCCCTC 
TTCAACATCG GCCTCCTCCT CTTCCTCGTC ATGTTCATCT ACTCCATCTT CGGCATGGCC 
AACTTTGCCT ACGTCAAGTG GGAGGCTGGC ATCGACGACA TGTTCAACTT CCAGACCTTT 
GCCAACAGCA TGCTGTGCCT TTTCCAGATC ACCACGTCGG CTGGCTGGGA TGGCCTCCTC 
AGCCCCATCC TCAACACAGG ACCCCCCTAC TGTGACCCCA ACCTGCCCAA CAGCAATGGC 
TCCAGGGGGA ACTGTGGGAG CCCAGCAGTG GGCATCCTCT TCTTCACCAC CTACATCATC 
ATCTCCTTCC TCATCGTGGT CAACATGTAC ATTGCCATCA TCCTGGAGAA CTTCAGTGTG 
GCCACAGAAG AGAGCACAGA GCCCCTGAGC GAGGATGACT TCGACATGTT CTATGAGATC 
TGGGAGAAGT TCGACCCGGA GGCCACCCAG TTCATTGAGT ATTTGGCCCT GTCCGACTTT 
GCCGATGCCC TGTCTGAGCC ACTCCGCATC GCCAAGCCCA ACCAGATAAG CCTCATCAAT 
ATGGACCTGC CCATGGTGAG CGGGGACCGC ATCCACTGTA TGGACATCCT GTTCGCCTTC 
ACCAAGAGGG TGCTCGGGGA GTCTGGGGAG ATGGATGCCC TGAAGATCCA GATGGAGGAG 
AAATTCATGG CGGCCAATCC TTCCAAGATC TCCTATGAGC CCATCACCAC CACCCTGCGG 
CGAAAACATG AGGAGGTGTC GGCCACGGTC ATCCAGCGGG CCTTCCGGAG GCACCTCCTG 
CAGCGCTCGG TGAAGCATGC TTCCTTCCTC TTCCGCCAGC AAGCGGGCAG CAGTGGCCTC 
TCTGATGAGG ATGCCCCCGA GCGAGAGGGA CTCATTGCCT ACATGATGAA TGAGAACTTC 
TCCCGGCGCA GCGGTCCCCT CTCCAGCTCT TCCATCTCCT CCACGTCCTT CCCACCGTCC 
TATGACAGCG TCACGAGGGC CACCAGTGAT AACCTCCCAG TGCGTGCGTC GGACTACAGC 
CGCAGCGAAG ATCTTGCAGA CTTCCCTCCA TCTCCAGATA GGGACCGAGA GTCTATAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_006511993.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512056.1 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X1, mRNA.

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CloneID	OMu43337
Clone ID Related Accession (Same CDS sequence)	XM_006511993.1
Accession Version	XM_006511993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGCAGGAATC CCAAGTTGTG TCTGGTGGCC ACGAGCCCCC CCAGGAGCCC 
AGGGCCTGGA GCCAGGTGTC AGAGACCACA TCCTCTGAAG CCGAGGCCAG TACGTCTCAG 
GCAGACTGGC AGCAAGAGCG GGAAGCGGAG CCCCGGGCCC CGGGGTGCGG TGAGACTCCC 
GAGGACAGTT ACTCCGAGGG TAGCACCGCT GACATGACCA ACACCGCAGA CCTCCTGGAG 
CAAATCCCAG ACCTCGGGGA GGACGTCAAG GACCCAGAGG ACTGCTTCAC TGAAGGCTGC 
GTCCGACGTT GTCCTTGCTG CATGGTGGAC ACGACGCAGG CCCCAGGGAA GGTCTGGTGG 
AGACTGCGCA AGACCTGCTA CCGCATAGTG GAGCACAGCT GGTTCGAGAC TTTCATCATC 
TTCATGATCC TGCTCAGCAG CGGAGCGCTG GCCTTCGAGG ACATCTACCT GGAGGAGCGG 
AAGACCATCA AGGTTCTGCT GGAGTACGCC GACAAGATGT TCACCTATGT CTTTGTGTTG 
GAGATGCTGC TCAAGTGGGT GGCCTACGGC TTCAAGAAGT ACTTCACCAA CGCCTGGTGC 
TGGCTGGACT TCCTAATTGT GGACGTCTCA CTGGTCAGCC TCGTGGCCAA CACCTTAGGC 
TTTGCCGAGA TGGGTCCCAT CAAGTCCCTG AGGACACTGC GTGCACTTCG ACCCCTGAGG 
GCTTTGTCAC GATTCGAGGG CATGAGGGTG GTGGTCAACG CGCTGGTGGG CGCCATCCCC 
TCCATCATGA ATGTCCTCCT CGTCTGCCTC ATCTTCTGGC TCATCTTCAG CATCATGGGC 
GTGAACCTCT TCGCCGGGAA GTTCGGTAGG TGCATCAACC AGACCGAAGG GGACCTGCCT 
CTGAACTACA CCATTGTGAA CAACAAGAGC GAGTGTGAGT CCTTCAACGT GACTGGAGAG 
TTGTACTGGA CCAAGGTGAA AGTCAACTTT GACAACGTGG GGGCTGGGTA CCTGGCCCTG 
CTGCAGGTGG CGACATTTAA AGGCTGGATG GACATCATGT ATGCGGCTGT GGACTCCAGA 
GGGTATGAAG AGCAGCCGCA GTGGGAGGAC AACCTCTACA TGTACATCTA CTTCGTCGTC 
TTCATCATCT TCGGCTCCTT CTTCACCCTC AACCTGTTTA TTGGTGTCAT CATTGATAAC 
TTCAACCAGC AGAAGAAAAA GTTAGGGGGC CAGGACATCT TCATGACGGA GGAGCAGAAG 
AAGTACTACA ATGCCATGAA GAAGCTGGGC TCCAAGAAAC CCCAGAAGCC CATCCCACGG 
CCTCTGAACA AGTACCAGGG TTTCATATTC GACATTGTGA CCAAGCAGGC CTTCGATGTT 
ACCATCATGT TCCTCATCTG TTTAAACATG GTGACCATGA TGGTAGAGAC AGATGATCAG 
AGCCCTGAAA AAGTCAACAT CTTGGCCAAG ATCAACCTGC TCTTCGTGGC CATCTTCACA 
GGAGAGTGTA TTGTCAAGAT GGCTGCCCTG CGCCACTATT ACTTCACCAA CAGCTGGAAC 
ATCTTCGATT TCGTGGTTGT CATCCTCTCC ATCGTTGGCA CTGTCCTCTC CGACATCATC 
CAGAAGTACT TCTTCTCCCC AACGCTCTTC CGTGTCATCC GTCTGGCCAG GATCGGCCGC 
ATCCTCAGGC TGATCCGAGG AGCCAAGGGG ATTCGCACGT TGCTCTTCGC CCTCATGATG 
TCCCTGCCCG CCCTCTTCAA CATCGGCCTC CTCCTCTTCC TCGTCATGTT CATCTACTCC 
ATCTTCGGCA TGGCCAACTT TGCCTACGTC AAGTGGGAGG CTGGCATCGA CGACATGTTC 
AACTTCCAGA CCTTTGCCAA CAGCATGCTG TGCCTTTTCC AGATCACCAC GTCGGCTGGC 
TGGGATGGCC TCCTCAGCCC CATCCTCAAC ACAGGACCCC CCTACTGTGA CCCCAACCTG 
CCCAACAGCA ATGGCTCCAG GGGGAACTGT GGGAGCCCAG CAGTGGGCAT CCTCTTCTTC 
ACCACCTACA TCATCATCTC CTTCCTCATC GTGGTCAACA TGTACATTGC CATCATCCTG 
GAGAACTTCA GTGTGGCCAC AGAAGAGAGC ACAGAGCCCC TGAGCGAGGA TGACTTCGAC 
ATGTTCTATG AGATCTGGGA GAAGTTCGAC CCGGAGGCCA CCCAGTTCAT TGAGTATTTG 
GCCCTGTCCG ACTTTGCCGA TGCCCTGTCT GAGCCACTCC GCATCGCCAA GCCCAACCAG 
ATAAGCCTCA TCAATATGGA CCTGCCCATG GTGAGCGGGG ACCGCATCCA CTGTATGGAC 
ATCCTGTTCG CCTTCACCAA GAGGGTGCTC GGGGAGTCTG GGGAGATGGA TGCCCTGAAG 
ATCCAGATGG AGGAGAAATT CATGGCGGCC AATCCTTCCA AGATCTCCTA TGAGCCCATC 
ACCACCACCC TGCGGCGAAA ACATGAGGAG GTGTCGGCCA CGGTCATCCA GCGGGCCTTC 
CGGAGGCACC TCCTGCAGCG CTCGGTGAAG CATGCTTCCT TCCTCTTCCG CCAGCAAGCG 
GGCAGCAGTG GCCTCTCTGA TGAGGATGCC CCCGAGCGAG AGGGACTCAT TGCCTACATG 
ATGAATGAGA ACTTCTCCCG GCGCAGCGGT CCCCTCTCCA GCTCTTCCAT CTCCTCCACG 
TCCTTCCCAC CGTCCTATGA CAGCGTCACG AGGGCCACCA GTGATAACCT CCCAGTGCGT 
GCGTCGGACT ACAGCCGCAG CGAAGATCTT GCAGACTTCC CTCCATCTCC AGATAGGGAC 
CGAGAGTCTA TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512056.1
CDS	72..6329
Translation	MEVYTVGHTRCQHLDASSCVPVRPAVAENPGSLGGQMLPPVAASMPQLPSRQPEESLCPQKQDEKMANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_006511993.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511993.1

ORF Insert Sequence:

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGCAGGAATC CCAAGTTGTG TCTGGTGGCC ACGAGCCCCC CCAGGAGCCC 
AGGGCCTGGA GCCAGGTGTC AGAGACCACA TCCTCTGAAG CCGAGGCCAG TACGTCTCAG 
GCAGACTGGC AGCAAGAGCG GGAAGCGGAG CCCCGGGCCC CGGGGTGCGG TGAGACTCCC 
GAGGACAGTT ACTCCGAGGG TAGCACCGCT GACATGACCA ACACCGCAGA CCTCCTGGAG 
CAAATCCCAG ACCTCGGGGA GGACGTCAAG GACCCAGAGG ACTGCTTCAC TGAAGGCTGC 
GTCCGACGTT GTCCTTGCTG CATGGTGGAC ACGACGCAGG CCCCAGGGAA GGTCTGGTGG 
AGACTGCGCA AGACCTGCTA CCGCATAGTG GAGCACAGCT GGTTCGAGAC TTTCATCATC 
TTCATGATCC TGCTCAGCAG CGGAGCGCTG GCCTTCGAGG ACATCTACCT GGAGGAGCGG 
AAGACCATCA AGGTTCTGCT GGAGTACGCC GACAAGATGT TCACCTATGT CTTTGTGTTG 
GAGATGCTGC TCAAGTGGGT GGCCTACGGC TTCAAGAAGT ACTTCACCAA CGCCTGGTGC 
TGGCTGGACT TCCTAATTGT GGACGTCTCA CTGGTCAGCC TCGTGGCCAA CACCTTAGGC 
TTTGCCGAGA TGGGTCCCAT CAAGTCCCTG AGGACACTGC GTGCACTTCG ACCCCTGAGG 
GCTTTGTCAC GATTCGAGGG CATGAGGGTG GTGGTCAACG CGCTGGTGGG CGCCATCCCC 
TCCATCATGA ATGTCCTCCT CGTCTGCCTC ATCTTCTGGC TCATCTTCAG CATCATGGGC 
GTGAACCTCT TCGCCGGGAA GTTCGGTAGG TGCATCAACC AGACCGAAGG GGACCTGCCT 
CTGAACTACA CCATTGTGAA CAACAAGAGC GAGTGTGAGT CCTTCAACGT GACTGGAGAG 
TTGTACTGGA CCAAGGTGAA AGTCAACTTT GACAACGTGG GGGCTGGGTA CCTGGCCCTG 
CTGCAGGTGG CGACATTTAA AGGCTGGATG GACATCATGT ATGCGGCTGT GGACTCCAGA 
GGGTATGAAG AGCAGCCGCA GTGGGAGGAC AACCTCTACA TGTACATCTA CTTCGTCGTC 
TTCATCATCT TCGGCTCCTT CTTCACCCTC AACCTGTTTA TTGGTGTCAT CATTGATAAC 
TTCAACCAGC AGAAGAAAAA GTTAGGGGGC CAGGACATCT TCATGACGGA GGAGCAGAAG 
AAGTACTACA ATGCCATGAA GAAGCTGGGC TCCAAGAAAC CCCAGAAGCC CATCCCACGG 
CCTCTGAACA AGTACCAGGG TTTCATATTC GACATTGTGA CCAAGCAGGC CTTCGATGTT 
ACCATCATGT TCCTCATCTG TTTAAACATG GTGACCATGA TGGTAGAGAC AGATGATCAG 
AGCCCTGAAA AAGTCAACAT CTTGGCCAAG ATCAACCTGC TCTTCGTGGC CATCTTCACA 
GGAGAGTGTA TTGTCAAGAT GGCTGCCCTG CGCCACTATT ACTTCACCAA CAGCTGGAAC 
ATCTTCGATT TCGTGGTTGT CATCCTCTCC ATCGTTGGCA CTGTCCTCTC CGACATCATC 
CAGAAGTACT TCTTCTCCCC AACGCTCTTC CGTGTCATCC GTCTGGCCAG GATCGGCCGC 
ATCCTCAGGC TGATCCGAGG AGCCAAGGGG ATTCGCACGT TGCTCTTCGC CCTCATGATG 
TCCCTGCCCG CCCTCTTCAA CATCGGCCTC CTCCTCTTCC TCGTCATGTT CATCTACTCC 
ATCTTCGGCA TGGCCAACTT TGCCTACGTC AAGTGGGAGG CTGGCATCGA CGACATGTTC 
AACTTCCAGA CCTTTGCCAA CAGCATGCTG TGCCTTTTCC AGATCACCAC GTCGGCTGGC 
TGGGATGGCC TCCTCAGCCC CATCCTCAAC ACAGGACCCC CCTACTGTGA CCCCAACCTG 
CCCAACAGCA ATGGCTCCAG GGGGAACTGT GGGAGCCCAG CAGTGGGCAT CCTCTTCTTC 
ACCACCTACA TCATCATCTC CTTCCTCATC GTGGTCAACA TGTACATTGC CATCATCCTG 
GAGAACTTCA GTGTGGCCAC AGAAGAGAGC ACAGAGCCCC TGAGCGAGGA TGACTTCGAC 
ATGTTCTATG AGATCTGGGA GAAGTTCGAC CCGGAGGCCA CCCAGTTCAT TGAGTATTTG 
GCCCTGTCCG ACTTTGCCGA TGCCCTGTCT GAGCCACTCC GCATCGCCAA GCCCAACCAG 
ATAAGCCTCA TCAATATGGA CCTGCCCATG GTGAGCGGGG ACCGCATCCA CTGTATGGAC 
ATCCTGTTCG CCTTCACCAA GAGGGTGCTC GGGGAGTCTG GGGAGATGGA TGCCCTGAAG 
ATCCAGATGG AGGAGAAATT CATGGCGGCC AATCCTTCCA AGATCTCCTA TGAGCCCATC 
ACCACCACCC TGCGGCGAAA ACATGAGGAG GTGTCGGCCA CGGTCATCCA GCGGGCCTTC 
CGGAGGCACC TCCTGCAGCG CTCGGTGAAG CATGCTTCCT TCCTCTTCCG CCAGCAAGCG 
GGCAGCAGTG GCCTCTCTGA TGAGGATGCC CCCGAGCGAG AGGGACTCAT TGCCTACATG 
ATGAATGAGA ACTTCTCCCG GCGCAGCGGT CCCCTCTCCA GCTCTTCCAT CTCCTCCACG 
TCCTTCCCAC CGTCCTATGA CAGCGTCACG AGGGCCACCA GTGATAACCT CCCAGTGCGT 
GCGTCGGACT ACAGCCGCAG CGAAGATCTT GCAGACTTCC CTCCATCTCC AGATAGGGAC 
CGAGAGTCTA TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_006511995.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512058.1 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X2, mRNA.

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CloneID	OMu43339
Clone ID Related Accession (Same CDS sequence)	XM_006511995.1
Accession Version	XM_006511995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGGAATCCCA AGTTGTGTCT GGTGGCCACG AGCCCCCCCA GGAGCCCAGG 
GCCTGGAGCC AGGTGTCAGA GACCACATCC TCTGAAGCCG AGGCCAGTAC GTCTCAGGCA 
GACTGGCAGC AAGAGCGGGA AGCGGAGCCC CGGGCCCCGG GGTGCGGTGA GACTCCCGAG 
GACAGTTACT CCGAGGGTAG CACCGCTGAC ATGACCAACA CCGCAGACCT CCTGGAGCAA 
ATCCCAGACC TCGGGGAGGA CGTCAAGGAC CCAGAGGACT GCTTCACTGA AGGCTGCGTC 
CGACGTTGTC CTTGCTGCAT GGTGGACACG ACGCAGGCCC CAGGGAAGGT CTGGTGGAGA 
CTGCGCAAGA CCTGCTACCG CATAGTGGAG CACAGCTGGT TCGAGACTTT CATCATCTTC 
ATGATCCTGC TCAGCAGCGG AGCGCTGGCC TTCGAGGACA TCTACCTGGA GGAGCGGAAG 
ACCATCAAGG TTCTGCTGGA GTACGCCGAC AAGATGTTCA CCTATGTCTT TGTGTTGGAG 
ATGCTGCTCA AGTGGGTGGC CTACGGCTTC AAGAAGTACT TCACCAACGC CTGGTGCTGG 
CTGGACTTCC TAATTGTGGA CGTCTCACTG GTCAGCCTCG TGGCCAACAC CTTAGGCTTT 
GCCGAGATGG GTCCCATCAA GTCCCTGAGG ACACTGCGTG CACTTCGACC CCTGAGGGCT 
TTGTCACGAT TCGAGGGCAT GAGGGTGGTG GTCAACGCGC TGGTGGGCGC CATCCCCTCC 
ATCATGAATG TCCTCCTCGT CTGCCTCATC TTCTGGCTCA TCTTCAGCAT CATGGGCGTG 
AACCTCTTCG CCGGGAAGTT CGGTAGGTGC ATCAACCAGA CCGAAGGGGA CCTGCCTCTG 
AACTACACCA TTGTGAACAA CAAGAGCGAG TGTGAGTCCT TCAACGTGAC TGGAGAGTTG 
TACTGGACCA AGGTGAAAGT CAACTTTGAC AACGTGGGGG CTGGGTACCT GGCCCTGCTG 
CAGGTGGCGA CATTTAAAGG CTGGATGGAC ATCATGTATG CGGCTGTGGA CTCCAGAGGG 
TATGAAGAGC AGCCGCAGTG GGAGGACAAC CTCTACATGT ACATCTACTT CGTCGTCTTC 
ATCATCTTCG GCTCCTTCTT CACCCTCAAC CTGTTTATTG GTGTCATCAT TGATAACTTC 
AACCAGCAGA AGAAAAAGTT AGGGGGCCAG GACATCTTCA TGACGGAGGA GCAGAAGAAG 
TACTACAATG CCATGAAGAA GCTGGGCTCC AAGAAACCCC AGAAGCCCAT CCCACGGCCT 
CTGAACAAGT ACCAGGGTTT CATATTCGAC ATTGTGACCA AGCAGGCCTT CGATGTTACC 
ATCATGTTCC TCATCTGTTT AAACATGGTG ACCATGATGG TAGAGACAGA TGATCAGAGC 
CCTGAAAAAG TCAACATCTT GGCCAAGATC AACCTGCTCT TCGTGGCCAT CTTCACAGGA 
GAGTGTATTG TCAAGATGGC TGCCCTGCGC CACTATTACT TCACCAACAG CTGGAACATC 
TTCGATTTCG TGGTTGTCAT CCTCTCCATC GTTGGCACTG TCCTCTCCGA CATCATCCAG 
AAGTACTTCT TCTCCCCAAC GCTCTTCCGT GTCATCCGTC TGGCCAGGAT CGGCCGCATC 
CTCAGGCTGA TCCGAGGAGC CAAGGGGATT CGCACGTTGC TCTTCGCCCT CATGATGTCC 
CTGCCCGCCC TCTTCAACAT CGGCCTCCTC CTCTTCCTCG TCATGTTCAT CTACTCCATC 
TTCGGCATGG CCAACTTTGC CTACGTCAAG TGGGAGGCTG GCATCGACGA CATGTTCAAC 
TTCCAGACCT TTGCCAACAG CATGCTGTGC CTTTTCCAGA TCACCACGTC GGCTGGCTGG 
GATGGCCTCC TCAGCCCCAT CCTCAACACA GGACCCCCCT ACTGTGACCC CAACCTGCCC 
AACAGCAATG GCTCCAGGGG GAACTGTGGG AGCCCAGCAG TGGGCATCCT CTTCTTCACC 
ACCTACATCA TCATCTCCTT CCTCATCGTG GTCAACATGT ACATTGCCAT CATCCTGGAG 
AACTTCAGTG TGGCCACAGA AGAGAGCACA GAGCCCCTGA GCGAGGATGA CTTCGACATG 
TTCTATGAGA TCTGGGAGAA GTTCGACCCG GAGGCCACCC AGTTCATTGA GTATTTGGCC 
CTGTCCGACT TTGCCGATGC CCTGTCTGAG CCACTCCGCA TCGCCAAGCC CAACCAGATA 
AGCCTCATCA ATATGGACCT GCCCATGGTG AGCGGGGACC GCATCCACTG TATGGACATC 
CTGTTCGCCT TCACCAAGAG GGTGCTCGGG GAGTCTGGGG AGATGGATGC CCTGAAGATC 
CAGATGGAGG AGAAATTCAT GGCGGCCAAT CCTTCCAAGA TCTCCTATGA GCCCATCACC 
ACCACCCTGC GGCGAAAACA TGAGGAGGTG TCGGCCACGG TCATCCAGCG GGCCTTCCGG 
AGGCACCTCC TGCAGCGCTC GGTGAAGCAT GCTTCCTTCC TCTTCCGCCA GCAAGCGGGC 
AGCAGTGGCC TCTCTGATGA GGATGCCCCC GAGCGAGAGG GACTCATTGC CTACATGATG 
AATGAGAACT TCTCCCGGCG CAGCGGTCCC CTCTCCAGCT CTTCCATCTC CTCCACGTCC 
TTCCCACCGT CCTATGACAG CGTCACGAGG GCCACCAGTG ATAACCTCCC AGTGCGTGCG 
TCGGACTACA GCCGCAGCGA AGATCTTGCA GACTTCCCTC CATCTCCAGA TAGGGACCGA 
GAGTCTATAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512058.1
CDS	72..6326
Translation	MEVYTVGHTRCQHLDASSCVPVRPAVAENPGSLGGQMLPPVAASMPQLPSRQPEESLCPQKQDEKMANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQEREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_006511995.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511995.1

ORF Insert Sequence:

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGGAATCCCA AGTTGTGTCT GGTGGCCACG AGCCCCCCCA GGAGCCCAGG 
GCCTGGAGCC AGGTGTCAGA GACCACATCC TCTGAAGCCG AGGCCAGTAC GTCTCAGGCA 
GACTGGCAGC AAGAGCGGGA AGCGGAGCCC CGGGCCCCGG GGTGCGGTGA GACTCCCGAG 
GACAGTTACT CCGAGGGTAG CACCGCTGAC ATGACCAACA CCGCAGACCT CCTGGAGCAA 
ATCCCAGACC TCGGGGAGGA CGTCAAGGAC CCAGAGGACT GCTTCACTGA AGGCTGCGTC 
CGACGTTGTC CTTGCTGCAT GGTGGACACG ACGCAGGCCC CAGGGAAGGT CTGGTGGAGA 
CTGCGCAAGA CCTGCTACCG CATAGTGGAG CACAGCTGGT TCGAGACTTT CATCATCTTC 
ATGATCCTGC TCAGCAGCGG AGCGCTGGCC TTCGAGGACA TCTACCTGGA GGAGCGGAAG 
ACCATCAAGG TTCTGCTGGA GTACGCCGAC AAGATGTTCA CCTATGTCTT TGTGTTGGAG 
ATGCTGCTCA AGTGGGTGGC CTACGGCTTC AAGAAGTACT TCACCAACGC CTGGTGCTGG 
CTGGACTTCC TAATTGTGGA CGTCTCACTG GTCAGCCTCG TGGCCAACAC CTTAGGCTTT 
GCCGAGATGG GTCCCATCAA GTCCCTGAGG ACACTGCGTG CACTTCGACC CCTGAGGGCT 
TTGTCACGAT TCGAGGGCAT GAGGGTGGTG GTCAACGCGC TGGTGGGCGC CATCCCCTCC 
ATCATGAATG TCCTCCTCGT CTGCCTCATC TTCTGGCTCA TCTTCAGCAT CATGGGCGTG 
AACCTCTTCG CCGGGAAGTT CGGTAGGTGC ATCAACCAGA CCGAAGGGGA CCTGCCTCTG 
AACTACACCA TTGTGAACAA CAAGAGCGAG TGTGAGTCCT TCAACGTGAC TGGAGAGTTG 
TACTGGACCA AGGTGAAAGT CAACTTTGAC AACGTGGGGG CTGGGTACCT GGCCCTGCTG 
CAGGTGGCGA CATTTAAAGG CTGGATGGAC ATCATGTATG CGGCTGTGGA CTCCAGAGGG 
TATGAAGAGC AGCCGCAGTG GGAGGACAAC CTCTACATGT ACATCTACTT CGTCGTCTTC 
ATCATCTTCG GCTCCTTCTT CACCCTCAAC CTGTTTATTG GTGTCATCAT TGATAACTTC 
AACCAGCAGA AGAAAAAGTT AGGGGGCCAG GACATCTTCA TGACGGAGGA GCAGAAGAAG 
TACTACAATG CCATGAAGAA GCTGGGCTCC AAGAAACCCC AGAAGCCCAT CCCACGGCCT 
CTGAACAAGT ACCAGGGTTT CATATTCGAC ATTGTGACCA AGCAGGCCTT CGATGTTACC 
ATCATGTTCC TCATCTGTTT AAACATGGTG ACCATGATGG TAGAGACAGA TGATCAGAGC 
CCTGAAAAAG TCAACATCTT GGCCAAGATC AACCTGCTCT TCGTGGCCAT CTTCACAGGA 
GAGTGTATTG TCAAGATGGC TGCCCTGCGC CACTATTACT TCACCAACAG CTGGAACATC 
TTCGATTTCG TGGTTGTCAT CCTCTCCATC GTTGGCACTG TCCTCTCCGA CATCATCCAG 
AAGTACTTCT TCTCCCCAAC GCTCTTCCGT GTCATCCGTC TGGCCAGGAT CGGCCGCATC 
CTCAGGCTGA TCCGAGGAGC CAAGGGGATT CGCACGTTGC TCTTCGCCCT CATGATGTCC 
CTGCCCGCCC TCTTCAACAT CGGCCTCCTC CTCTTCCTCG TCATGTTCAT CTACTCCATC 
TTCGGCATGG CCAACTTTGC CTACGTCAAG TGGGAGGCTG GCATCGACGA CATGTTCAAC 
TTCCAGACCT TTGCCAACAG CATGCTGTGC CTTTTCCAGA TCACCACGTC GGCTGGCTGG 
GATGGCCTCC TCAGCCCCAT CCTCAACACA GGACCCCCCT ACTGTGACCC CAACCTGCCC 
AACAGCAATG GCTCCAGGGG GAACTGTGGG AGCCCAGCAG TGGGCATCCT CTTCTTCACC 
ACCTACATCA TCATCTCCTT CCTCATCGTG GTCAACATGT ACATTGCCAT CATCCTGGAG 
AACTTCAGTG TGGCCACAGA AGAGAGCACA GAGCCCCTGA GCGAGGATGA CTTCGACATG 
TTCTATGAGA TCTGGGAGAA GTTCGACCCG GAGGCCACCC AGTTCATTGA GTATTTGGCC 
CTGTCCGACT TTGCCGATGC CCTGTCTGAG CCACTCCGCA TCGCCAAGCC CAACCAGATA 
AGCCTCATCA ATATGGACCT GCCCATGGTG AGCGGGGACC GCATCCACTG TATGGACATC 
CTGTTCGCCT TCACCAAGAG GGTGCTCGGG GAGTCTGGGG AGATGGATGC CCTGAAGATC 
CAGATGGAGG AGAAATTCAT GGCGGCCAAT CCTTCCAAGA TCTCCTATGA GCCCATCACC 
ACCACCCTGC GGCGAAAACA TGAGGAGGTG TCGGCCACGG TCATCCAGCG GGCCTTCCGG 
AGGCACCTCC TGCAGCGCTC GGTGAAGCAT GCTTCCTTCC TCTTCCGCCA GCAAGCGGGC 
AGCAGTGGCC TCTCTGATGA GGATGCCCCC GAGCGAGAGG GACTCATTGC CTACATGATG 
AATGAGAACT TCTCCCGGCG CAGCGGTCCC CTCTCCAGCT CTTCCATCTC CTCCACGTCC 
TTCCCACCGT CCTATGACAG CGTCACGAGG GCCACCAGTG ATAACCTCCC AGTGCGTGCG 
TCGGACTACA GCCGCAGCGA AGATCTTGCA GACTTCCCTC CATCTCCAGA TAGGGACCGA 
GAGTCTATAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_006511996.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512059.1 Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X3, mRNA.

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CloneID	OMu43340
Clone ID Related Accession (Same CDS sequence)	XM_006511996.1
Accession Version	XM_006511996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 5 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGACTCCCGA GGACAGTTAC TCCGAGGGTA GCACCGCTGA CATGACCAAC 
ACCGCAGACC TCCTGGAGCA AATCCCAGAC CTCGGGGAGG ACGTCAAGGA CCCAGAGGAC 
TGCTTCACTG AAGGCTGCGT CCGACGTTGT CCTTGCTGCA TGGTGGACAC GACGCAGGCC 
CCAGGGAAGG TCTGGTGGAG ACTGCGCAAG ACCTGCTACC GCATAGTGGA GCACAGCTGG 
TTCGAGACTT TCATCATCTT CATGATCCTG CTCAGCAGCG GAGCGCTGGC CTTCGAGGAC 
ATCTACCTGG AGGAGCGGAA GACCATCAAG GTTCTGCTGG AGTACGCCGA CAAGATGTTC 
ACCTATGTCT TTGTGTTGGA GATGCTGCTC AAGTGGGTGG CCTACGGCTT CAAGAAGTAC 
TTCACCAACG CCTGGTGCTG GCTGGACTTC CTAATTGTGG ACGTCTCACT GGTCAGCCTC 
GTGGCCAACA CCTTAGGCTT TGCCGAGATG GGTCCCATCA AGTCCCTGAG GACACTGCGT 
GCACTTCGAC CCCTGAGGGC TTTGTCACGA TTCGAGGGCA TGAGGGTGGT GGTCAACGCG 
CTGGTGGGCG CCATCCCCTC CATCATGAAT GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC 
ATCTTCAGCA TCATGGGCGT GAACCTCTTC GCCGGGAAGT TCGGTAGGTG CATCAACCAG 
ACCGAAGGGG ACCTGCCTCT GAACTACACC ATTGTGAACA ACAAGAGCGA GTGTGAGTCC 
TTCAACGTGA CTGGAGAGTT GTACTGGACC AAGGTGAAAG TCAACTTTGA CAACGTGGGG 
GCTGGGTACC TGGCCCTGCT GCAGGTGGCG ACATTTAAAG GCTGGATGGA CATCATGTAT 
GCGGCTGTGG ACTCCAGAGG GTATGAAGAG CAGCCGCAGT GGGAGGACAA CCTCTACATG 
TACATCTACT TCGTCGTCTT CATCATCTTC GGCTCCTTCT TCACCCTCAA CCTGTTTATT 
GGTGTCATCA TTGATAACTT CAACCAGCAG AAGAAAAAGT TAGGGGGCCA GGACATCTTC 
ATGACGGAGG AGCAGAAGAA GTACTACAAT GCCATGAAGA AGCTGGGCTC CAAGAAACCC 
CAGAAGCCCA TCCCACGGCC TCTGAACAAG TACCAGGGTT TCATATTCGA CATTGTGACC 
AAGCAGGCCT TCGATGTTAC CATCATGTTC CTCATCTGTT TAAACATGGT GACCATGATG 
GTAGAGACAG ATGATCAGAG CCCTGAAAAA GTCAACATCT TGGCCAAGAT CAACCTGCTC 
TTCGTGGCCA TCTTCACAGG AGAGTGTATT GTCAAGATGG CTGCCCTGCG CCACTATTAC 
TTCACCAACA GCTGGAACAT CTTCGATTTC GTGGTTGTCA TCCTCTCCAT CGTTGGCACT 
GTCCTCTCCG ACATCATCCA GAAGTACTTC TTCTCCCCAA CGCTCTTCCG TGTCATCCGT 
CTGGCCAGGA TCGGCCGCAT CCTCAGGCTG ATCCGAGGAG CCAAGGGGAT TCGCACGTTG 
CTCTTCGCCC TCATGATGTC CCTGCCCGCC CTCTTCAACA TCGGCCTCCT CCTCTTCCTC 
GTCATGTTCA TCTACTCCAT CTTCGGCATG GCCAACTTTG CCTACGTCAA GTGGGAGGCT 
GGCATCGACG ACATGTTCAA CTTCCAGACC TTTGCCAACA GCATGCTGTG CCTTTTCCAG 
ATCACCACGT CGGCTGGCTG GGATGGCCTC CTCAGCCCCA TCCTCAACAC AGGACCCCCC 
TACTGTGACC CCAACCTGCC CAACAGCAAT GGCTCCAGGG GGAACTGTGG GAGCCCAGCA 
GTGGGCATCC TCTTCTTCAC CACCTACATC ATCATCTCCT TCCTCATCGT GGTCAACATG 
TACATTGCCA TCATCCTGGA GAACTTCAGT GTGGCCACAG AAGAGAGCAC AGAGCCCCTG 
AGCGAGGATG ACTTCGACAT GTTCTATGAG ATCTGGGAGA AGTTCGACCC GGAGGCCACC 
CAGTTCATTG AGTATTTGGC CCTGTCCGAC TTTGCCGATG CCCTGTCTGA GCCACTCCGC 
ATCGCCAAGC CCAACCAGAT AAGCCTCATC AATATGGACC TGCCCATGGT GAGCGGGGAC 
CGCATCCACT GTATGGACAT CCTGTTCGCC TTCACCAAGA GGGTGCTCGG GGAGTCTGGG 
GAGATGGATG CCCTGAAGAT CCAGATGGAG GAGAAATTCA TGGCGGCCAA TCCTTCCAAG 
ATCTCCTATG AGCCCATCAC CACCACCCTG CGGCGAAAAC ATGAGGAGGT GTCGGCCACG 
GTCATCCAGC GGGCCTTCCG GAGGCACCTC CTGCAGCGCT CGGTGAAGCA TGCTTCCTTC 
CTCTTCCGCC AGCAAGCGGG CAGCAGTGGC CTCTCTGATG AGGATGCCCC CGAGCGAGAG 
GGACTCATTG CCTACATGAT GAATGAGAAC TTCTCCCGGC GCAGCGGTCC CCTCTCCAGC 
TCTTCCATCT CCTCCACGTC CTTCCCACCG TCCTATGACA GCGTCACGAG GGCCACCAGT 
GATAACCTCC CAGTGCGTGC GTCGGACTAC AGCCGCAGCG AAGATCTTGC AGACTTCCCT 
CCATCTCCAG ATAGGGACCG AGAGTCTATA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512059.1
CDS	72..6167
Translation	MEVYTVGHTRCQHLDASSCVPVRPAVAENPGSLGGQMLPPVAASMPQLPSRQPEESLCPQKQDEKMANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVPIAVAESDTDDQEEDEENSLGTEEEESSKQTPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENFSRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_006511996.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type V, alpha (Scn5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511996.1

ORF Insert Sequence:

ATGGAGGTCT ACACAGTTGG GCACACCAGA TGCCAACATC TGGATGCTAG TTCTTGTGTT 
CCTGTCCGGC CGGCCGTTGC TGAAAACCCT GGATCCCTTG GGGGGCAAAT GCTGCCTCCA 
GTTGCTGCCT CTATGCCTCA GCTTCCTTCC AGGCAGCCTG AGGAGAGCCT GTGCCCCCAG 
AAGCAGGATG AGAAGATGGC AAACTTCCTG TTACCTCGGG GCACCAGCAG CTTCCGTAGG 
TTCACCCGGG AGTCACTGGC GGCCATCGAG AAGCGCATGG CTGAAAAACA AGCCCGTGGT 
TCGGCCACCT CACAGGAGAG CCGTGAGGGC CTGCCAGAGG AGGAGGCTCC CCGGCCCCAG 
CTGGACCTAC AGGCCTCCAA AAAGCTGCCA GATCTCTATG GCAACCCACC CCGAGAGCTC 
ATTGGGGAGC CCCTGGAAGA CCTGGACCCT TTCTATAGTA CCCAGAAGAC CTTCATCGTG 
CTGAATAAGG GCAAAACCAT CTTCCGGTTC AGTGCCACCA ATGCCTTGTA CGTCCTCAGC 
CCCTTCCACC CGGTGCGCAG AGCGGCTGTG AAGATTTTGG TACATTCGCT CTTCAGCATG 
CTCATCATGT GCACCATCCT AACCAACTGT GTGTTCATGG CACAACACGA CCCTCCGCCT 
TGGACCAAAT ATGTTGAGTA CACCTTCACC GCCATCTACA CCTTTGAGTC TCTGGTCAAG 
ATTCTAGCTC GAGGCTTCTG CCTGCATGCG TTCACCTTCC TCCGGGACCC GTGGAACTGG 
CTAGACTTCA GTGTGATCGT CATGGCATAC ACAACTGAAT TTGTGGACCT GGGCAATGTC 
TCAGCCTTAC GCACCTTCCG AGTCCTCCGG GCCCTGAAAA CTATATCGGT CATTTCAGGC 
CTGAAGACAA TCGTGGGAGC CCTAATCCAG TCTGTGAAGA AGCTAGCCGA TGTGATGGTC 
CTTACTGTCT TCTGCCTCAG TGTCTTTGCC CTCATTGGCC TGCAGCTCTT CATGGGCAAC 
CTGAGGCACA AGTGCGTGCG TAACTTTACT GAGCTCAATG GCACCAATGG TTCCGTGGAG 
GCCGATGGCA TAGTCTGGAA CTCTCTGGAC GTCTACCTCA ATGACCCAGC CAATTACCTA 
CTCAAGAATG GCACCACGGA TGTGTTGCTG TGTGGGAACA GCTCTGATGC CGGGACATGT 
CCTGAAGGCT ACCGGTGCCT GAAGGCAGGT GAGAACCCTG ACCACGGTTA CACCAGCTTC 
GACTCCTTCG CCTGGGCTTT CCTTGCGCTC TTCCGCCTGA TGACACAGGA CTGCTGGGAA 
CGTCTATACC AGCAGACCCT GAGGTCCGCA GGAAAGATCT ACATGATCTT CTTCATGCTT 
GTCATATTCC TGGGCTCCTT CTACCTTGTG AACTTGATCC TGGCTGTGGT GGCCATGGCT 
TACGAGGAGC AAAACCAAGC CACCATCGCC GAGACGGAAG AGAAGGAGAA GCGTTTCCAG 
GAGGCCATGG AGATGCTCAA GAAGGAACAC GAGGCTCTCA CCATCAGGGG CGTGGATACT 
GTGTCCCGTA GCTCTCTGGA GATGTCTCCT TTGGCCCCAG TAACCAACCA TGAGAGAAGG 
AGCAAGAGGA GGAAACGACT GTCTTCAGGG ACAGAGGATG GCGGAGATGA CAGGCTCCCC 
AAGTCGGACT CAGAAGACGG TCCCAGAGCA TTGAATCAGC TCAGCCTCAC CCATGGGCTC 
AGCCGGACAT CCATGAGGCC CCGCTCCAGC CGAGGGAGCA TTTTCACATT CCGGCGACGG 
GACCAAGGCT CCGAGGCAGA TTTTGCAGAT GACGAGAACA GTACAGCAGG AGAGAGCGAG 
AGCCACCGCA CATCGCTGCT GGTGCCCTGG CCCCTGCGCC GTCCCAGCAC CCAAGGGCAG 
CCCGGCTTTG GAACCTCAGC TCCTGGTCAT GTCCTCAATG GCAAAAGAAA CAGCACCGTG 
GACTGCAATG GGGTGGTTTC CTTGCTGGGG GCAGGGGACG CAGAGGCCAC CTCTCCAGGG 
AGCCACCTTC TCCGCCCTAT AGTGCTGGAC AGACCCCCAG ACACGACCAC GCCGTCAGAG 
GAGCCGGGTG GGCCCCAGAT GCTGACACCG CAGGCTCCAT GTGCAGATGG CTTTGAGGAG 
CCCGGGGCAC GGCAACGGGC ACTCAGCGCC GTCAGTGTCC TCACCAGCGC TCTGGAAGAG 
TTGGAGGAGT CCCACCGTAA GTGTCCACCA TGCTGGAATC GCTTTGCCCA GCATTACCTC 
ATCTGGGAGT GCTGCCCACT CTGGATGTCC ATCAAGCAGA AGGTGAAGTT CGTGGTCATG 
GACCCGTTTG CTGACCTCAC CATCACCATG TGCATCGTGC TCAACACGCT CTTCATGGCT 
CTGGAGCACT ACAACATGAC AGCCGAGTTT GAGGAGATGC TGCAGGTCGG AAACTTGGTC 
TTCACGGGAA TCTTCACGGC AGAGATGACC TTCAAGATCA TCGCCCTTGA CCCATACTAC 
TACTTCCAGC AGGGCTGGAA TATCTTCGAC AGCATCATCG TCATCCTCAG CCTCATGGAG 
CTGGGCCTGT CTCGCATGGG CAACCTGTCT GTGCTGCGTT CCTTCCGTCT GCTGCGGGTC 
TTCAAGCTGG CCAAATCATG GCCCACCCTG AACACACTCA TCAAGATCAT CGGGAACTCT 
GTTGGTGCCC TGGGGAACCT GACCCTGGTG CTGGCCATCA TCGTCTTCAT CTTCGCCGTG 
GTGGGTATGC AGCTCTTCGG CAAGAACTAC TCAGAGCTGA GGCACCGAAT CAGCGACTCT 
GGCCTGCTGC CCCGCTGGCA CATGATGGAC TTCTTCCACG CCTTCCTCAT CATCTTCCGC 
ATCCTCTGTG GAGAGTGGAT CGAGACCATG TGGGACTGCA TGGAGGTGTC TGGGCAGTCG 
CTGTGCTTGC TGGTCTTCCT GCTGGTCATG GTCATTGGCA ACCTTGTGGT CCTGAATCTC 
TTCTTGGCCT TGCTGCTCAG CTCCTTCAGC GCAGACAACC TCACAGCCCC TGACGAGGAT 
GGGGAGATGA ACAACCTCCA GCTGGCCCTG GCTCGCATCC AAAGGGGCCT TCGCTTTGTC 
AAGCGGACCA CCTGGGACTT CTGCTGCGGG CTCCTGCGGC GGCGACCTAA GAAGCCCGCG 
GCTCTTGCCA CCCACAGCCA GCTGCCCAGC TGTATCGCCG CTCCCAGGTC CCCACCACCC 
CCAGAGGTGG AGAAGGCGCC CCCAGCCCGC AAGGAAACAC GGTTCGAGGA AGACAAGCGG 
CCGGGCCAGG GCACCCCTGG GGATACCGAG CCTGTGTGTG TTCCCATCGC AGTGGCTGAG 
TCAGACACTG ATGACCAGGA AGAGGATGAG GAGAACAGCC TTGGCACGGA GGAGGAAGAG 
TCCAGCAAAC AGACTCCCGA GGACAGTTAC TCCGAGGGTA GCACCGCTGA CATGACCAAC 
ACCGCAGACC TCCTGGAGCA AATCCCAGAC CTCGGGGAGG ACGTCAAGGA CCCAGAGGAC 
TGCTTCACTG AAGGCTGCGT CCGACGTTGT CCTTGCTGCA TGGTGGACAC GACGCAGGCC 
CCAGGGAAGG TCTGGTGGAG ACTGCGCAAG ACCTGCTACC GCATAGTGGA GCACAGCTGG 
TTCGAGACTT TCATCATCTT CATGATCCTG CTCAGCAGCG GAGCGCTGGC CTTCGAGGAC 
ATCTACCTGG AGGAGCGGAA GACCATCAAG GTTCTGCTGG AGTACGCCGA CAAGATGTTC 
ACCTATGTCT TTGTGTTGGA GATGCTGCTC AAGTGGGTGG CCTACGGCTT CAAGAAGTAC 
TTCACCAACG CCTGGTGCTG GCTGGACTTC CTAATTGTGG ACGTCTCACT GGTCAGCCTC 
GTGGCCAACA CCTTAGGCTT TGCCGAGATG GGTCCCATCA AGTCCCTGAG GACACTGCGT 
GCACTTCGAC CCCTGAGGGC TTTGTCACGA TTCGAGGGCA TGAGGGTGGT GGTCAACGCG 
CTGGTGGGCG CCATCCCCTC CATCATGAAT GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC 
ATCTTCAGCA TCATGGGCGT GAACCTCTTC GCCGGGAAGT TCGGTAGGTG CATCAACCAG 
ACCGAAGGGG ACCTGCCTCT GAACTACACC ATTGTGAACA ACAAGAGCGA GTGTGAGTCC 
TTCAACGTGA CTGGAGAGTT GTACTGGACC AAGGTGAAAG TCAACTTTGA CAACGTGGGG 
GCTGGGTACC TGGCCCTGCT GCAGGTGGCG ACATTTAAAG GCTGGATGGA CATCATGTAT 
GCGGCTGTGG ACTCCAGAGG GTATGAAGAG CAGCCGCAGT GGGAGGACAA CCTCTACATG 
TACATCTACT TCGTCGTCTT CATCATCTTC GGCTCCTTCT TCACCCTCAA CCTGTTTATT 
GGTGTCATCA TTGATAACTT CAACCAGCAG AAGAAAAAGT TAGGGGGCCA GGACATCTTC 
ATGACGGAGG AGCAGAAGAA GTACTACAAT GCCATGAAGA AGCTGGGCTC CAAGAAACCC 
CAGAAGCCCA TCCCACGGCC TCTGAACAAG TACCAGGGTT TCATATTCGA CATTGTGACC 
AAGCAGGCCT TCGATGTTAC CATCATGTTC CTCATCTGTT TAAACATGGT GACCATGATG 
GTAGAGACAG ATGATCAGAG CCCTGAAAAA GTCAACATCT TGGCCAAGAT CAACCTGCTC 
TTCGTGGCCA TCTTCACAGG AGAGTGTATT GTCAAGATGG CTGCCCTGCG CCACTATTAC 
TTCACCAACA GCTGGAACAT CTTCGATTTC GTGGTTGTCA TCCTCTCCAT CGTTGGCACT 
GTCCTCTCCG ACATCATCCA GAAGTACTTC TTCTCCCCAA CGCTCTTCCG TGTCATCCGT 
CTGGCCAGGA TCGGCCGCAT CCTCAGGCTG ATCCGAGGAG CCAAGGGGAT TCGCACGTTG 
CTCTTCGCCC TCATGATGTC CCTGCCCGCC CTCTTCAACA TCGGCCTCCT CCTCTTCCTC 
GTCATGTTCA TCTACTCCAT CTTCGGCATG GCCAACTTTG CCTACGTCAA GTGGGAGGCT 
GGCATCGACG ACATGTTCAA CTTCCAGACC TTTGCCAACA GCATGCTGTG CCTTTTCCAG 
ATCACCACGT CGGCTGGCTG GGATGGCCTC CTCAGCCCCA TCCTCAACAC AGGACCCCCC 
TACTGTGACC CCAACCTGCC CAACAGCAAT GGCTCCAGGG GGAACTGTGG GAGCCCAGCA 
GTGGGCATCC TCTTCTTCAC CACCTACATC ATCATCTCCT TCCTCATCGT GGTCAACATG 
TACATTGCCA TCATCCTGGA GAACTTCAGT GTGGCCACAG AAGAGAGCAC AGAGCCCCTG 
AGCGAGGATG ACTTCGACAT GTTCTATGAG ATCTGGGAGA AGTTCGACCC GGAGGCCACC 
CAGTTCATTG AGTATTTGGC CCTGTCCGAC TTTGCCGATG CCCTGTCTGA GCCACTCCGC 
ATCGCCAAGC CCAACCAGAT AAGCCTCATC AATATGGACC TGCCCATGGT GAGCGGGGAC 
CGCATCCACT GTATGGACAT CCTGTTCGCC TTCACCAAGA GGGTGCTCGG GGAGTCTGGG 
GAGATGGATG CCCTGAAGAT CCAGATGGAG GAGAAATTCA TGGCGGCCAA TCCTTCCAAG 
ATCTCCTATG AGCCCATCAC CACCACCCTG CGGCGAAAAC ATGAGGAGGT GTCGGCCACG 
GTCATCCAGC GGGCCTTCCG GAGGCACCTC CTGCAGCGCT CGGTGAAGCA TGCTTCCTTC 
CTCTTCCGCC AGCAAGCGGG CAGCAGTGGC CTCTCTGATG AGGATGCCCC CGAGCGAGAG 
GGACTCATTG CCTACATGAT GAATGAGAAC TTCTCCCGGC GCAGCGGTCC CCTCTCCAGC 
TCTTCCATCT CCTCCACGTC CTTCCCACCG TCCTATGACA GCGTCACGAG GGCCACCAGT 
GATAACCTCC CAGTGCGTGC GTCGGACTAC AGCCGCAGCG AAGATCTTGC AGACTTCCCT 
CCATCTCCAG ATAGGGACCG AGAGTCTATA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo.
Nature communications10(1)4943(2019 10)
Man JCK,Mohan RA,Boogaard MVD,Hilvering CRE,Jenkins C,Wakker V,Bianchi V,Laat W,Barnett P,Boukens BJ,Christoffels VM

Autonomic modulation of the electrical substrate in mice haploinsufficient for cardiac sodium channels: a model of the Brugada syndrome.
American journal of physiology. Cell physiology317(3)C576-C583(2019 Sep)
Finlay M,Bhar-Amato J,Ng KE,Santos D,Orini M,Vyas V,Taggart P,Grace AA,Huang CL,Lambiase PD,Tinker A

Protein Phosphatase 2A Regulates Cardiac Na
Circulation research124(5)737-746(2019 03)
El Refaey M,Musa H,Murphy NP,Lubbers ER,Skaf M,Han M,Cavus O,Koenig SN,Wallace MJ,Gratz D,Bradley E,Alsina KM,Wehrens XHT,Hund TJ,Mohler PJ

Gene-environment interaction impacts on heart development and embryo survival.
Development (Cambridge, England)146(4)(2019 02)
Moreau JLM,Kesteven S,Martin EMMA,Lau KS,Yam MX,O'Reilly VC,Del Monte-Nieto G,Baldini A,Feneley MP,Moon AM,Harvey RP,Sparrow DB,Chapman G,Dunwoodie SL

CRISPR -Mediated Expression of the Fetal Scn5a Isoform in Adult Mice Causes Conduction Defects and Arrhythmias.
Journal of the American Heart Association7(19)e010393(2018 10)
Pang PD,Alsina KM,Cao S,Koushik AB,Wehrens XHT,Cooper TA

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Scn5a cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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