Rapsn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rapsn
Entrez Gene ID	19400
Full Name	receptor-associated protein of the synapse
Synonyms	43kDa,Nraps,Raps,rapsyn
General protein information	Preferred Names receptor-associated protein of the synapse Names 43 kDa receptor-associated protein of the synapse 43 kDa postsynaptic protein 43kDa acetylcholine receptor-associated protein acetylcholine receptor-associated 43 kDa protein receptor-associated protein of the synapse, 43 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E1\|2 50.44 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009023.3	NP_033049.2	43 kDa receptor-associated protein of the synapse
XM_006499017.3	XP_006499080.1	43 kDa receptor-associated protein of the synapse isoform X1
XM_006499017.4	XP_006499080.1	43 kDa receptor-associated protein of the synapse isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAPSN	NP_005046.2
Pan troglodytes (chimpanzee)	RAPSN	XP_001168947.1
Caenorhabditis elegans (roundworm)	rpy-1	NP_495365.1
Gallus gallus (chicken)	RAPSN	NP_990428.1
Danio rerio (zebrafish)	rapsn	NP_840090.1
Xenopus tropicalis (tropical clawed frog)	rapsn	NP_001005667.1
Bos taurus (cattle)	RAPSN	NP_001178350.1
Rattus norvegicus (Norway rat)	Rapsn	NP_001102054.1
Drosophila melanogaster (fruit fly)	CG1909	NP_726586.1
Canis lupus familiaris (dog)	RAPSN	XP_850095.1
Mus musculus (house mouse)	Rapsn	NP_033049.2

Gene Ontology
Bibliography

Related articles in PubMed

MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.
Oury J, Liu Y, Töpf A, Todorovic S, Hoedt E, Preethish-Kumar V, Neubert TA, Lin W, Lochmüller H, Burden SJ
The Journal of cell biology218(5)1686-1705(2019 May)

Gene expression profiling in the developing secondary palate in the absence of Tbx1 function.
Zoupa M, Xavier GM, Bryan S, Theologidis I, Arno M, Cobourne MT
BMC genomics19(1)429(2018 Jun)

Enzymatic Activity of the Scaffold Protein Rapsyn for Synapse Formation.
Li L, Cao Y, Wu H, Ye X, Zhu Z, Xing G, Shen C, Barik A, Zhang B, Xie X, Zhi W, Gan L, Su H, Xiong WC, Mei L
Neuron92(5)1007-1019(2016 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

AChRs Are Essential for the Targeting of Rapsyn to the Postsynaptic Membrane of NMJs in Living Mice.
Chen PJ, Martinez-Pena Y Valenzuela I, Aittaleb M, Akaaboune M
The Journal of neuroscience : the official journal of the Society for Neuroscience36(21)5680-5(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rapsn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapsn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03336	NM_009023.3 Latest version!	Mus musculus receptor-associated protein of the synapse (Rapsn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu30167	XM_006499017.4 Latest version!	Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu30167	XM_006499017.3	Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rapsn ( NM_009023.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033049.2 Mus musculus receptor-associated protein of the synapse (Rapsn), mRNA.

Price & Availability↑

CloneID	OMu03336
Clone ID Related Accession (Same CDS sequence)	NM_009023.3
Accession Version	NM_009023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Mus musculus(house mouse)
Product	43 kDa receptor-associated protein of the synapse
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY709325.1 and J03962.1. On Mar 13, 2009 this sequence version replaced NM_009023.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15788.1, BC111863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGGCTTTGGA TGCCATTGAG AAAGCCCAGG ACTTAGCTGA AGAGGTTGGC 
AATAAGCTGA GCCAGCTCAA GCTGCATTGC CTGAGTGAGA GCATCTACCG CAGCAAAGGG 
CTGCAGCGTG AGCTGCGCAC GCACGTAGTG AGGTTCCACG AGTGCGTGGA GGAGACTGAG 
CTCTACTGCG GCCTCTGTGG TGAGTCCATC GGGGAGAGGA ACAGCCGGCT GCAGGCCCTG 
CCCTGCTCCC ACATCTTTCA TCTCAGATGC CTGCAAAACA ATGGCACTAG GAGCTGCCCC 
AACTGCCGCC GCTCCTCCAT GAAGCCGGGC TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033049.2
CDS	171..1409
Translation	MGQDQTKQQIEKGLQLYQSNQTEKALQVWMKVLEKGSDLVGRFRVLGCLVTAHSEMGRYKEMLKFAVVQIDTARGLEDADFLLESYLNLARSNEKLCEFHKTISYCKTCLGLPGTRAGAQLGGQVSLSMGNAFLGLSLFQKALESFEKALRYAHNNDDTMLECRVCCSLGSFYAQVKDYEKALFFPCKAAELVNDYGKGWSLKYRAMSQYHMAVAYRLLGHLGSAMECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIGNRLGQVHVLLGVAKCWMARKVQDKALDAIEKAQDLAEEVGNKLSQLKLHCLSESIYRSKGLQRELRTHVVRFHECVEETELYCGLCGESIGERNSRLQALPCSHIFHLRCLQNNGTRSCPNCRRSSMKPGFV

Target ORF information:

RefSeq Version	NM_009023.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus receptor-associated protein of the synapse (Rapsn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009023.3

ORF Insert Sequence:

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGGCTTTGGA TGCCATTGAG AAAGCCCAGG ACTTAGCTGA AGAGGTTGGC 
AATAAGCTGA GCCAGCTCAA GCTGCATTGC CTGAGTGAGA GCATCTACCG CAGCAAAGGG 
CTGCAGCGTG AGCTGCGCAC GCACGTAGTG AGGTTCCACG AGTGCGTGGA GGAGACTGAG 
CTCTACTGCG GCCTCTGTGG TGAGTCCATC GGGGAGAGGA ACAGCCGGCT GCAGGCCCTG 
CCCTGCTCCC ACATCTTTCA TCTCAGATGC CTGCAAAACA ATGGCACTAG GAGCTGCCCC 
AACTGCCGCC GCTCCTCCAT GAAGCCGGGC TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapsn ( XM_006499017.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499080.1 Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30167
Clone ID Related Accession (Same CDS sequence)	XM_006499017.3 , XM_006499017.4
Accession Version	XM_006499017.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	43 kDa receptor-associated protein of the synapse isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGCTGAGCCA GCTCAAGCTG CATTGCCTGA GTGAGAGCAT CTACCGCAGC 
AAAGGGCTGC AGCGTGAGCT GCGCACGCAC GTAGTGAGGT TCCACGAGTG CGTGGAGGAG 
ACTGAGCTCT ACTGCGGCCT CTGTGGTGAG TCCATCGGGG AGAGGAACAG CCGGCTGCAG 
GCCCTGCCCT GCTCCCACAT CTTTCATCTC AGATGCCTGC AAAACAATGG CACTAGGAGC 
TGCCCCAACT GCCGCCGCTC CTCCATGAAG CCGGGCTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499080.1
CDS	339..1523
Translation	MGQDQTKQQIEKGLQLYQSNQTEKALQVWMKVLEKGSDLVGRFRVLGCLVTAHSEMGRYKEMLKFAVVQIDTARGLEDADFLLESYLNLARSNEKLCEFHKTISYCKTCLGLPGTRAGAQLGGQVSLSMGNAFLGLSLFQKALESFEKALRYAHNNDDTMLECRVCCSLGSFYAQVKDYEKALFFPCKAAELVNDYGKGWSLKYRAMSQYHMAVAYRLLGHLGSAMECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIGNRLGQVHVLLGVAKCWMARKVQDKLSQLKLHCLSESIYRSKGLQRELRTHVVRFHECVEETELYCGLCGESIGERNSRLQALPCSHIFHLRCLQNNGTRSCPNCRRSSMKPGFV

Target ORF information:

RefSeq Version	XM_006499017.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499017.3

ORF Insert Sequence:

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGCTGAGCCA GCTCAAGCTG CATTGCCTGA GTGAGAGCAT CTACCGCAGC 
AAAGGGCTGC AGCGTGAGCT GCGCACGCAC GTAGTGAGGT TCCACGAGTG CGTGGAGGAG 
ACTGAGCTCT ACTGCGGCCT CTGTGGTGAG TCCATCGGGG AGAGGAACAG CCGGCTGCAG 
GCCCTGCCCT GCTCCCACAT CTTTCATCTC AGATGCCTGC AAAACAATGG CACTAGGAGC 
TGCCCCAACT GCCGCCGCTC CTCCATGAAG CCGGGCTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapsn ( XM_006499017.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499080.1 Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30167
Clone ID Related Accession (Same CDS sequence)	XM_006499017.3 , XM_006499017.4
Accession Version	XM_006499017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	43 kDa receptor-associated protein of the synapse isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499017.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGCTGAGCCA GCTCAAGCTG CATTGCCTGA GTGAGAGCAT CTACCGCAGC 
AAAGGGCTGC AGCGTGAGCT GCGCACGCAC GTAGTGAGGT TCCACGAGTG CGTGGAGGAG 
ACTGAGCTCT ACTGCGGCCT CTGTGGTGAG TCCATCGGGG AGAGGAACAG CCGGCTGCAG 
GCCCTGCCCT GCTCCCACAT CTTTCATCTC AGATGCCTGC AAAACAATGG CACTAGGAGC 
TGCCCCAACT GCCGCCGCTC CTCCATGAAG CCGGGCTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499080.1
CDS	326..1510
Translation	MGQDQTKQQIEKGLQLYQSNQTEKALQVWMKVLEKGSDLVGRFRVLGCLVTAHSEMGRYKEMLKFAVVQIDTARGLEDADFLLESYLNLARSNEKLCEFHKTISYCKTCLGLPGTRAGAQLGGQVSLSMGNAFLGLSLFQKALESFEKALRYAHNNDDTMLECRVCCSLGSFYAQVKDYEKALFFPCKAAELVNDYGKGWSLKYRAMSQYHMAVAYRLLGHLGSAMECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIGNRLGQVHVLLGVAKCWMARKVQDKLSQLKLHCLSESIYRSKGLQRELRTHVVRFHECVEETELYCGLCGESIGERNSRLQALPCSHIFHLRCLQNNGTRSCPNCRRSSMKPGFV

Target ORF information:

RefSeq Version	XM_006499017.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus receptor-associated protein of the synapse (Rapsn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499017.4

ORF Insert Sequence:

ATGGGGCAGG ACCAGACAAA GCAACAGATT GAAAAAGGAC TGCAGCTGTA CCAGTCCAAC 
CAGACAGAGA AGGCACTGCA GGTGTGGATG AAGGTGCTGG AGAAGGGCTC TGACCTCGTG 
GGCCGCTTCC GGGTACTGGG CTGCTTGGTA ACAGCTCACT CGGAGATGGG CCGCTACAAA 
GAGATGCTGA AGTTTGCCGT GGTCCAGATT GATACTGCTC GGGGACTGGA GGATGCTGAC 
TTCCTGCTCG AAAGCTACCT GAACCTGGCG CGCAGCAATG AGAAGCTATG TGAGTTCCAC 
AAAACCATCT CCTACTGCAA GACCTGCCTC GGCCTGCCTG GCACCAGGGC TGGTGCCCAG 
CTTGGGGGTC AGGTCAGCCT GAGCATGGGC AATGCTTTCC TGGGCCTCAG CCTCTTCCAG 
AAGGCCCTGG AGAGCTTTGA GAAGGCCCTG CGCTATGCCC ACAACAACGA TGACACCATG 
CTGGAGTGCC GTGTCTGCTG CAGCCTGGGC AGTTTCTACG CCCAGGTCAA GGACTATGAG 
AAAGCCCTGT TCTTTCCCTG CAAGGCTGCA GAGCTTGTCA ACGACTATGG CAAAGGCTGG 
AGCCTCAAAT ATCGGGCCAT GAGCCAGTAC CACATGGCTG TGGCCTACCG CCTGCTGGGC 
CACCTGGGCA GTGCCATGGA GTGTTGTGAG GAGTCCATGA AGATTGCCCT GCAGCACGGT 
GACCGCCCGC TACAGGCACT CTGTCTGCTC TGCTTTGCCG ATATCCATCG GAGCCGAGGG 
GACCTGGAGA CAGCCTTCCC TCGGTACGAC TCCGCTATGA GCATCATGAC TGAGATCGGA 
AACCGCCTCG GGCAGGTGCA CGTGCTGCTG GGTGTGGCCA AGTGCTGGAT GGCCCGGAAG 
GTGCAAGACA AGCTGAGCCA GCTCAAGCTG CATTGCCTGA GTGAGAGCAT CTACCGCAGC 
AAAGGGCTGC AGCGTGAGCT GCGCACGCAC GTAGTGAGGT TCCACGAGTG CGTGGAGGAG 
ACTGAGCTCT ACTGCGGCCT CTGTGGTGAG TCCATCGGGG AGAGGAACAG CCGGCTGCAG 
GCCCTGCCCT GCTCCCACAT CTTTCATCTC AGATGCCTGC AAAACAATGG CACTAGGAGC 
TGCCCCAACT GCCGCCGCTC CTCCATGAAG CCGGGCTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.
The Journal of cell biology218(5)1686-1705(2019 May)
Oury J,Liu Y,Töpf A,Todorovic S,Hoedt E,Preethish-Kumar V,Neubert TA,Lin W,Lochmüller H,Burden SJ

Gene expression profiling in the developing secondary palate in the absence of Tbx1 function.
BMC genomics19(1)429(2018 Jun)
Zoupa M,Xavier GM,Bryan S,Theologidis I,Arno M,Cobourne MT

Enzymatic Activity of the Scaffold Protein Rapsyn for Synapse Formation.
Neuron92(5)1007-1019(2016 Dec)
Li L,Cao Y,Wu H,Ye X,Zhu Z,Xing G,Shen C,Barik A,Zhang B,Xie X,Zhi W,Gan L,Su H,Xiong WC,Mei L

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

AChRs Are Essential for the Targeting of Rapsyn to the Postsynaptic Membrane of NMJs in Living Mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(21)5680-5(2016 05)
Chen PJ,Martinez-Pena Y Valenzuela I,Aittaleb M,Akaaboune M

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Rapsn cDNA ORF clone, Mus musculus(house mouse)

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